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Biochemistry and Molecular Biology Education

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https://www.readbyqxmd.com/read/28205321/an-investigative-graduate-laboratory-course-for-teaching-modern-dna-techniques
#1
Alexandre de Lencastre, A Thomas Torello, Lani C Keller
This graduate-level DNA methods laboratory course is designed to model a discovery-based research project and engages students in both traditional DNA analysis methods and modern recombinant DNA cloning techniques. In the first part of the course, students clone the Drosophila ortholog of a human disease gene of their choosing using Gateway(®) cloning. In the second part of the course, students examine the expression of their gene of interest in human cell lines by reverse transcription PCR and learn how to analyze data from quantitative reverse transcription PCR (qRT-PCR) experiments...
February 16, 2017: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/28145107/a-comprehensive-experiment-for-molecular-biology-determination-of-single-nucleotide-polymorphism-in-human-rev3-gene-using-pcr-rflp
#2
Xu Zhang, Meng Shao, Lu Gao, Yuanyuan Zhao, Zixuan Sun, Liping Zhou, Yongmin Yan, Qixiang Shao, Wenrong Xu, Hui Qian
Laboratory exercise is helpful for medical students to understand the basic principles of molecular biology and to learn about the practical applications of molecular biology. We have designed a lab course on molecular biology about the determination of single nucleotide polymorphism (SNP) in human REV3 gene, the product of which is a subunit of DNA polymerase ζ and SNPs in this gene are associated with altered susceptibility to cancer. This newly designed experiment is composed of three parts, including genomic DNA extraction, gene amplification by PCR, and genotyping by RFLP...
February 1, 2017: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/28145077/using-molecular-visualization-to-explore-protein-structure-and-function-and-enhance-student-facility-with-computational-tools
#3
Cassidy R Terrell, Laura L Listenberger
Recognizing that undergraduate students can benefit from analysis of 3D protein structure and function, we have developed a multiweek, inquiry-based molecular visualization project for Biochemistry I students. This project uses a virtual model of cyclooxygenase-1 (COX-1) to guide students through multiple levels of protein structure analysis. The first assignment explores primary structure by generating and examining a protein sequence alignment. Subsequent assignments introduce 3D visualization software to explore secondary, tertiary, and quaternary structure...
February 1, 2017: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/28145074/assessment-of-knowledge-of-participants-on-basic-molecular-biology-techniques-after-5-day-intensive-molecular-biology-training-workshops-in-nigeria
#4
J I Yisau, A O Adagbada, T Bamidele, M Fowora, B I C Brai, O Adebesin, M Bamidele, T Fesobi, F O Nwaokorie, A Ajayi, S I Smith
The deployment of molecular biology techniques for diagnosis and research in Nigeria is faced with a number of challenges, including the cost of equipment and reagents coupled with the dearth of personnel skilled in the procedures and handling of equipment. Short molecular biology training workshops were conducted at the Nigerian Institute of Medical Research (NIMR), to improve the knowledge and skills of laboratory personnel and academics in health, research, and educational facilities. Five-day molecular biology workshops were conducted annually between 2011 and 2014, with participants drawn from health, research facilities, and the academia...
February 1, 2017: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/28059472/promoting-active-learning-of-graduate-student-by-deep-reading-in-biochemistry-and-microbiology-pharmacy-curriculum
#5
Ren Peng
To promote graduate students' active learning, deep reading of high quality papers was done by graduate students enrolled in biochemistry and microbiology pharmacy curriculum offered by college of life science, Jiangxi Normal University from 2013 to 2015. The number of graduate students, who participated in the course in 2013, 2014, and 2015 were eleven, thirteen and fifteen, respectively. Through deep reading of papers, presentation, and group discussion in the lecture, these graduate students have improved their academic performances effectively, such as literature search, PPT document production, presentation management, specialty document reading, academic inquiry, and analytical and comprehensive ability...
January 6, 2017: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/28032413/variation-in-external-representations-as-part-of-the-classroom-lecture-an-investigation-of-virtual-cell-animations-in-introductory-photosynthesis-instruction
#6
Eric E Goff, Katie M Reindl, Christina Johnson, Phillip McClean, Erika G Offerdahl, Noah L Schroeder, Alan R White
The use of external representations (ERs) to introduce concepts in undergraduate biology has become increasingly common. Two of the most prevalent are static images and dynamic animations. While previous studies comparing static images and dynamic animations have resulted in somewhat conflicting findings in regards to learning outcomes, the benefits of each have been shown individually. Using ERs developed by the Virtual Cell Animation project, we aim to further investigate student learning using different ERs as part of an introductory biology lecture...
December 28, 2016: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/28032403/a-unique-large-scale-undergraduate-research-experience-in-molecular-systems-biology-for-non-mathematics-majors
#7
Ulrike Kappler, Susan L Rowland, Rhianna K Pedwell
Systems biology is frequently taught with an emphasis on mathematical modeling approaches. This focus effectively excludes most biology, biochemistry, and molecular biology students, who are not mathematics majors. The mathematical focus can also present a misleading picture of systems biology, which is a multi-disciplinary pursuit requiring collaboration between biochemists, bioinformaticians, and mathematicians. This article describes an authentic large-scale undergraduate research experience (ALURE) in systems biology that incorporates proteomics, bacterial genomics, and bioinformatics in the one exercise...
December 28, 2016: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/27925371/effective-visual-design-and-communication-practices-for-research-posters-exemplars-based-on-the-theory-and-practice-of-multimedia-learning-and-rhetoric
#8
Rhianna K Pedwell, James A Hardy, Susan L Rowland
Evidence shows that science graduates often do not have the communication skills they need to meet workplace standards and expectations. One common mode of science communication is the poster. In a review of the literature we show that poster design is historically problematic, and that the guidance provided to students as they create posters for assessment is frequently inconsistent. To address this inconsistency we provide some guiding design principles for posters that are grounded in communication theory and the fundamentals of rhetoric...
December 7, 2016: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/27910203/designing-easy-dna-extraction-teaching-creativity-through-laboratory-practice
#9
Endang Susantini, Lisa Lisdiana, Isnawati, Aushia Tanzih Al Haq, Guntur Trimulyono
Subject material concerning Deoxyribose Nucleic Acid (DNA) structure in the format of creativity-driven laboratory practice offers meaningful learning experience to the students. Therefore, a laboratory practice in which utilizes simple procedures and easy-safe-affordable household materials should be promoted to students to develop their creativity. This study aimed to examine whether designing and conducting DNA extraction with household materials could foster students' creative thinking. We also described how this laboratory practice affected students' knowledge and views...
December 2, 2016: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/27910201/meal-insulin-cycle-a-visual-summary-of-the-biochemical-events-between-meals
#10
Stavros Kalogiannis
In the present article, a scheme that summarizes the biochemical events occurring in the human body after the consumption of a meal is proposed. The scheme illustrates the metabolic sequence as a series of counteracting components occupying opposite positions in a cycle, indicating their opposite actions or physiological states, such as meal versus energy consumption, increase versus decrease of blood glucose levels, and excretion of insulin versus excretion of glucagon. Furthermore, the cycle is complemented by a table indicating the acceleration or inhibition of selected metabolic pathways and actions...
December 2, 2016: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/28101912/meetings-calendar
#11
(no author information available yet)
No abstract text is available yet for this article.
January 2, 2017: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/27486685/an-expanded-framework-for-biomolecular-visualization-in-the-classroom-learning-goals-and-competencies
#12
Daniel R Dries, Diane M Dean, Laura L Listenberger, Walter R P Novak, Margaret A Franzen, Paul A Craig
A thorough understanding of the molecular biosciences requires the ability to visualize and manipulate molecules in order to interpret results or to generate hypotheses. While many instructors in biochemistry and molecular biology use visual representations, few indicate that they explicitly teach visual literacy. One reason is the need for a list of core content and competencies to guide a more deliberate instruction in visual literacy. We offer here the second stage in the development of one such resource for biomolecular three-dimensional visual literacy...
January 2, 2017: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/27357493/embedding-responsible-conduct-in-learning-and-research-into-an-australian-undergraduate-curriculum
#13
Lynette B Fernandes
Responsible conduct in learning and research (RCLR) was progressively introduced into the pharmacology curriculum for undergraduate science students at The University of Western Australia. In the second year of this undergraduate curriculum, a lecture introduces students to issues such as the use of animals in teaching and responsible conduct of research. Third year student groups deliver presentations on topics including scientific integrity and the use of human subjects in research. Academic and research staff attending these presentations provide feedback and participate in discussions...
January 2, 2017: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/27357379/cures-in-biochemistry-where-we-are-and-where-we-should-go
#14
EDITORIAL
Jessica K Bell, Todd T Eckdahl, David A Hecht, Patrick J Killion, Joachim Latzer, Tamara L Mans, Joseph J Provost, John F Rakus, Erica A Siebrasse, J Ellis Bell
Integration of research experience into classroom is an important and vital experience for all undergraduates. These course-based undergraduate research experiences (CUREs) have grown from independent instructor lead projects to large consortium driven experiences. The impact and importance of CUREs on students at all levels in biochemistry was the focus of a National Science Foundation funded think tank. The state of biochemistry CUREs and suggestions for moving biochemistry forward as well as a practical guide (supplementary material) are reported here...
January 2, 2017: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/27353527/arsenic-based-life-an-active-learning-assignment-for-teaching-scientific-discourse
#15
R Jeremy Johnson
Among recent high profile scientific debates was the proposal that life could exist with arsenic in place of phosphorous in its nucleic acids and other biomolecules. Soon after its initial publication, scientists across diverse disciplines began to question this extraordinary claim. Using the original article, its claims, its scientific support, and the ensuing counterarguments, a two-day, active learning classroom exercise was developed focusing on the presentation, evaluation, and discussion of scientific argumentation and discourse...
January 2, 2017: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/27322926/design-and-evaluation-of-a-digital-module-with-guided-peer-feedback-for-student-learning-biotechnology-and-molecular-life-sciences-attitudinal-change-and-satisfaction
#16
Omid Noroozi, Martin Mulder
This study aims to investigate the impacts of a digital learning module with guided peer feedback on students' domain-specific knowledge gain and their attitudinal change in the field of biotechnology and molecular life sciences. The extent to which the use of this module is appreciated by students is studied as well. A pre-test, post-test design was used with 203 students who were randomly assigned to groups of three. They were asked to work on the digital module with the aim of exploring various perspectives, and the "pros and cons" on the topic of "Genetically Modified Organisms (GMOs)...
January 2, 2017: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/27292873/of-responsible-research-exploring-the-science-society-dialogue-in-undergraduate-training-within-the-life-sciences
#17
Maria Strecht Almeida, Alexandre Quintanilha
We explore the integration of societal issues in undergraduate training within the life sciences. Skills in thinking about science, scientific knowledge production and the place of science in society are crucial in the context of the idea of responsible research and innovation. This idea became institutionalized and it is currently well-present in the scientific agenda. Developing abilities in this regard seems particularly relevant to training in the life sciences, as new developments in this area somehow evoke the involvement of all of us citizens, our engagement to debate and take part in processes of change...
January 2, 2017: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/27292587/pymol-mcontrol-manipulating-molecular-visualization-with-mobile-devices
#18
Wendy W T Lam, Shirley W I Siu
Viewing and manipulating three-dimensional (3D) structures in molecular graphics software are essential tasks for researchers and students to understand the functions of molecules. Currently, the way to manipulate a 3D molecular object is mainly based on mouse-and-keyboard control that is usually difficult and tedious to learn. While gesture-based and touch-based interactions are increasingly popular in interactive software systems, their suitability in handling molecular graphics has not yet been sufficiently explored...
January 2, 2017: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/27231204/the-translational-science-training-program-at-nih-introducing-early-career-researchers-to-the-science-and-operation-of-translation-of-basic-research-to-medical-interventions
#19
C Taylor Gilliland, G Sitta Sittampalam, Philip Y Wang, Philip E Ryan
Translational science is an emerging field that holds great promise to accelerate the development of novel medical interventions. As the field grows, so does the demand for highly trained biomedical scientists to fill the positions that are being created. Many graduate and postdoctorate training programs do not provide their trainees with sufficient education to take advantage of this growing employment sector. To help better prepare the trainees at the National Institutes of Health for possible careers in translation, we have created the Translational Science Training Program (TSTP)...
January 2, 2017: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/27229266/using-an-fplc-to-promote-active-learning-of-the-principles-of-protein-structure-and-purification
#20
Rebekah L Robinson, Amy E Neely, Wais Mojadedi, Katie N Threatt, Nicole Y Davis, Mitch H Weiland
The concepts of protein purification are often taught in undergraduate biology and biochemistry lectures and reinforced during laboratory exercises; however, very few reported activities allow students to directly gain experience using modern protein purification instruments, such as Fast Protein Liquid Chromatography (FPLC). This laboratory exercise uses size exclusion chromatography (SEC) and ion exchange (IEX) chromatography to separate a mixture of four different proteins. Students use an SEC chromatogram and corresponding SDS-PAGE gel to understand how protein conformations change under different conditions (i...
January 2, 2017: Biochemistry and Molecular Biology Education
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