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Biochemistry and Molecular Biology Education

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https://www.readbyqxmd.com/read/29732711/biochemistry-cure-lab-survey
#1
LETTER
Paul A Craig
No abstract text is available yet for this article.
May 6, 2018: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/29732677/creation-and-implementation-of-a-flipped-jigsaw-activity-to-stimulate-interest-in-biochemistry-among-medical-students
#2
Charlene Williams, Susan Perlis, John Gaughan, Sangita Phadtare
Learner-centered pedagogical methods that are based on clinical application of basic science concepts through active learning and problem solving are shown to be effective for improving knowledge retention. As the clinical relevance of biochemistry is not always apparent to health-profession students, effective teaching of medical biochemistry should highlight the implications of biochemical concepts in pathology, minimize memorization, and make the concepts memorable for long-term retention. Here, we report the creation and successful implementation of a flipped jigsaw activity that was developed to stimulate interest in learning biochemistry among medical students...
May 6, 2018: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/29717551/how-to-calculate-thermostability-of-enzymes-using-a-simple-approach
#3
Abdul A N Saqib, Khawar S Siddiqui
Determination of thermostability of enzymes is of prime importance for their successful industrial applications and, yet, the published data has often been incompletely analyzed to assess the suitability of enzymes. It is possible to determine meaningful thermostability parameters from the routinely acquired data through a straightforward method that is not only more informative but also provides a means to compare thermostability of enzymes from different sources. Here, we describe a simple, effective, and economical way to determine enzyme thermostability...
May 2, 2018: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/29717550/an-integrated-course-based-on-two-dimensional-electrophoresis-and-mass-spectrometry-analysis-to-teach-proteomic-techniques-for-undergraduate-students
#4
Shanshan Sha, Liqiu Jia, Yi Xin, Weili Yao, Yufang Ma
Here, we developed an integrated course based on two dimensional-electrophoresis and spectrometry mass (2DE-MS) technique for undergraduate students to help them learn proteomic techniques. The soluble proteins in wild type and gene knockout bacteria were separated by 2DE and the differently expressed proteins were identified by MS analysis. The proteomic data was finally confirmed by RT-PCR detection. The separated experiments of 2DE, MS, RNA isolation, RT-PCR, as well as essential bioinformatic analysis, were integrated into a one-week course, which provided students an opportunity to systematically understand the proteomic techniques and their applications in current scientific research...
May 2, 2018: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/29694708/aqueous-biphasic-systems-in-the-separation-of-food-colorants
#5
João H P M Santos, Emanuel V Capela, Isabel Boal-Palheiros, João A P Coutinho, Mara G Freire, Sónia P M Ventura
Aqueous biphasic systems (ABS) composed of polypropylene glycol and carbohydrates, two benign substances are proposed to separate two food colorants (E122 and E133). ABS are promising extractive platforms, particularly for biomolecules, due to their aqueous and mild nature (pH and temperature), reduced environmental impact and processing costs. Another major aspect considered, particularly useful in downstream processing, is the "tuning" ability for the extraction and purification of these systems by a proper choice of the ABS components...
April 25, 2018: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/29668075/a-comparison-of-debate-and-role-play-in-enhancing-critical-thinking-and-communication-skills-of-medical-students-during-problem-based-learning
#6
Rabia Latif, Sadaf Mumtaz, Rafia Mumtaz, Aamir Hussain
Debate and role play for learning critical thinking and communication skills are being increasingly used in various undergraduate medical schools worldwide. We aim to compare students' views about effectiveness of two teaching strategies; debate and role play to exercise critical thinking and communication skills during problem-based learning (PBL). This is a comparative, cross-sectional, and questionnaire-based study. Our subjects were second year undergraduate female medical students enrolled in Imam Abdulrahman Bin Faisal University (IAU), College of Medicine from September 2014-2016, divided into 10 small PBL groups (10-13 students/group/year)...
April 18, 2018: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/29528559/improving-large-class-performance-and-engagement-through-student-generated-question-banks
#7
Dale Hancock, Nicole Hare, Paul Denny, Gareth Denyer
Disciplines such as Biochemistry and Molecular Biology, which involve concepts not included in the high-school curriculum, are very challenging for many first year university students. These subjects are particularly difficult for students accustomed to surface learning strategies involving memorization and recall of facts, as a deeper understanding of the relationship between concepts is needed for successful transfer to related areas and subsequent study. In this article, we explore an activity in a very large first year Molecular Biology course, in which students create multiple-choice questions related to targeted learning outcomes, and then answer and evaluate one another's questions...
March 12, 2018: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/29528558/preparing-graduate-student-teaching-assistants-in-the-sciences-an-intensive-workshop-focused-on-active-learning
#8
Julie A Roden, Susanne Jakob, Casey Roehrig, Tamara J Brenner
In the past ten years, increasing evidence has demonstrated that scientific teaching and active learning improve student retention and learning gains in the sciences. Graduate teaching assistants (GTAs), who play an important role in undergraduate education at many universities, require training in these methods to encourage implementation, long-term adoption, and advocacy. Here, we describe the design and evaluation of a two-day training workshop for first-year GTAs in the life sciences. This workshop combines instruction in current research and theory supporting teaching science through active learning as well as opportunities for participants to practice teaching and receive feedback from peers and mentors...
March 12, 2018: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/29528553/design-of-a-drug-discovery-course-for-non-science-majors
#9
Danielle H Dube
"Drug Discovery" is a 13-week lecture and laboratory-based course that was developed to introduce non-science majors to foundational chemistry and biochemistry concepts as they relate to the unifying theme of drug discovery. The first part of this course strives to build students' understanding of molecules, their properties, the differences that enable them to be separated from one another, and their abilities to bind to biological receptors and elicit physiological effects. After building students' molecular worldview, the course then focuses on four classes of drugs: antimicrobials, drugs that affect the mind, steroid-based drugs, and anti-cancer drugs...
March 12, 2018: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/29512279/a-course-based-undergraduate-research-experience-investigating-the-consequences-of-nonconserved-mutations-in-lactate-dehydrogenase
#10
Allan Ayella, Moriah R Beck
There is a growing movement to involve undergraduate students in authentic research experiences. A variety of studies have indicated the strength of this approach in developing scientific aptitude, confidence, critical thinking skills, and increasing the likelihood to become career scientists. Course-based undergraduate research experiences (CUREs) foster opportunities for students to carry out authentic research at both primarily undergraduate and large research institutions. Here, we describe a novel CURE-based biochemistry laboratory course to explore the consequences of mutagenesis of non-conserved amino acid sites in the structure and function of the lactate dehydrogenase enzyme and demonstrate how collaborations between institutions can facilitate real research experiences at any type of institution...
March 7, 2018: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/29512269/from-gene-to-structure-lactobacillus-bulgaricus-d-lactate-dehydrogenase-from-yogurt-as-an-integrated-curriculum-model-for-undergraduate-molecular-biology-and-biochemistry-laboratory-courses
#11
Jeffrey A Lawton, Noelle A Prescott, Ping X Lawton
We have developed an integrated, project-oriented curriculum for undergraduate molecular biology and biochemistry laboratory courses spanning two semesters that is organized around the ldhA gene from the yogurt-fermenting bacterium Lactobacillus bulgaricus, which encodes the enzyme d-lactate dehydrogenase. The molecular biology module, which consists of nine experiments carried out over eleven sessions, begins with the isolation of genomic DNA from L. bulgaricus in yogurt and guides students through the process of cloning the ldhA gene into a prokaryotic expression vector, followed by mRNA isolation and characterization of recombinant gene expression levels using RT-PCR...
March 7, 2018: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/29488323/augmented-reality-experimentation-on-oxygen-gas-generation-from-hydrogen-peroxide-and-bleach-reaction
#12
Hong Seng Gan, Nicholas Yee Kwang Tee, Mohammad Raziun Bin Mamtaz, Kevin Xiao, Brandon Huey-Ping Cheong, Oi Wah Liew, Tuck Wah Ng
The appreciation and understanding of gas generation through processes is vital in biochemical education. In this work, an augmented reality tool is reported to depict the redox reaction between hydrogen peroxide and sodium hypochlorite solutions, two ubiquitous oxidizing agents, to create oxygen, a combustible gas. As it operates out of smartphones or tablets, students are able to conduct the exercise collaboratively, respond in a manner similar to an actual physical experiment, and able to depict the oxygen volume changes in relation to the volume of hydrogen peroxide of different concentrations used...
February 28, 2018: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/29488320/simple-and-rapid-system-for-two-dimensional-gel-electrophoresis-technique-a-laboratory-exercise-for-high-school-students
#13
Praveen Maurye, Arpita Basu, Jayanta Kumar Biswas, Tapas Kumar Bandyopadhyay, Malay Naskar
Polyacrylamide gel electrophoresis (PAGE) is the most classical technique favored worldwide for resolution of macromolecules in many biochemistry laboratories due to its incessant advanced developments and wide modifications. These ever-growing advancements in the basic laboratory equipments lead to emergence of many expensive, complex, and tricky laboratory equipments. Practical courses of biochemistry at high school or undergraduate levels are often affected by these complications. Two dimensional gel electrophoresis technique (2D-PAGE) used for resolving thousands of proteins in a gel is a combination of isoelectric focusing (first dimension gel electrophoresis technique) and sodium-dodecylsulphate PAGE (second dimension gel electrophoresis technique or SDS-PAGE)...
February 28, 2018: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/29473287/a-laboratory-exercise-for-detecting-microbial-contaminants
#14
Jie Shen, Xiang Zhu, Kai Jin, Ya Li, YiTian Gu, Jiahong Huang, Yulan Qu, Xiaoyu Li
The development and evaluation of a 6-hours laboratory class, based on capillary electrophoresis (CE) and the detection of microbial contaminants, is described. It can be easily scaled up or down, to suit class sizes up to 188 and completed in a shorter time scale. CE uses narrow-bore fused-silica capillaries to separate a complex array of large and small molecules. A laboratory exercise has been devised to illustrate how CE-based genetic analysis system processes DNA fragment analysis to detect three microbial contaminants...
February 23, 2018: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/29381252/molecular-biology-at-the-cutting-edge-a-review-on-crispr-cas9-gene-editing-for-undergraduates
#15
REVIEW
Deborah M Thurtle-Schmidt, Te-Wen Lo
Disrupting a gene to determine its effect on an organism's phenotype is an indispensable tool in molecular biology. Such techniques are critical for understanding how a gene product contributes to the development and cellular identity of organisms. The explosion of genomic sequencing technologies combined with recent advances in genome-editing techniques has elevated the possibilities of genetic manipulations in numerous organisms in which these experiments were previously not readily accessible or possible...
March 2018: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/29285862/grade-distribution-digests-a-novel-tool-to-enhance-teaching-and-student-learning-in-laboratory-practicals
#16
Peter G Arthur, Reza Zareie, Paul Kirkwood, Martha Ludwig, Paul V Attwood
Assessment is a central component of course curriculums and is used to certify student learning, but it can also be used as a tool to improve teaching and learning. Many laboratory courses are structured such that there is only a grade for a particular laboratory, which limits the insights that can be gained in student learning. We developed a laboratory program that incorporates assessments designed to probe student understanding of different components of the individual modules making up the program. The challenge was to analyze and present grades from these assessment tasks in a format that was readily interpretable by academics...
March 2018: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/29285857/update-on-research-and-application-of-problem-based-learning-in-medical-science-education
#17
Chuifeng Fan, Biying Jiang, Xiuying Shi, Enhua Wang, Qingchang Li
Problem-based learning (PBL) is a unique form of pedagogy dedicated to developing students' self-learning and clinical practice skills. After several decades of development, although applications vary, PBL has been recognized all over the world and implemented by many medical schools. This review summarizes and updates the application and study of PBL in medical education through the literature published between 1993 and early 2017. It focuses on understanding real medical PBL courses and ways to improve PBL to achieve better learning outcomes...
March 2018: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/29274256/small-laccase-from-streptomyces-coelicolor-an-ideal-model-protein-enzyme-for-undergraduate-laboratory-experience
#18
Ryan Cook, Drew Hannon, Jonathan N Southard, Sudipta Majumdar
A one semester undergraduate biochemistry laboratory experience is described for an understanding of recombinant technology from gene cloning to protein characterization. An integrated experimental design includes three sequential modules: molecular cloning, protein expression and purification, and protein analysis and characterization. Students perform the tasks of cloning, expression, purification, analysis, and characterization of small laccase (SLAC) from Streptomyces coelicolor. SLAC is an extremely robust well-characterized protein/enzyme, which serves as an ideal model for undergraduate teaching laboratories...
March 2018: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/29274248/stem-cell-education-for-medical-students-at-tongji-university-primary-cell-culture-and-directional-differentiation-of-rat-bone-marrow-mesenchymal-stem-cells
#19
Caixia Jin, Haibin Tian, Jiao Li, Song Jia, Siguang Li, Guo-Tong Xu, Lei Xu, Lixia Lu
Stem cells are cells that can self-renew and differentiate into a variety of cell types under certain conditions. Stem cells have great potential in regenerative medicine and cell therapy for the treatment of certain diseases. To deliver knowledge about this frontier in science and technology to medical undergraduate students, we designed an innovative practical experiment for freshmen in their second semester. The lab exercise focused on rat bone marrow mesenchymal stem cell (BMSC) isolation, cell culture and differentiation, and it aimed to help students master the aseptic techniques for cell culture, the basic methods and procedures for the primary culture and passage of BMSCs, the basic procedure for the directional differentiation of BMSCs into adipocytes and their subsequent identification by oil-red-O staining...
March 2018: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/29215191/comparison-of-two-different-techniques-of-cooperative-learning-approach-undergraduates-conceptual-understanding-in-the-context-of-hormone-biochemistry
#20
Ayfer Mutlu
The purpose of the research was to compare the effects of two different techniques of the cooperative learning approach, namely Team-Game Tournament and Jigsaw, on undergraduates' conceptual understanding in a Hormone Biochemistry course. Undergraduates were randomly assigned to Group 1 (N = 23) and Group 2 (N = 29). Instructions were accomplished using Team-Game Tournament in Group 1 and Jigsaw in Group 2. Before the instructions, all groups were informed about cooperative learning and techniques, their responsibilities in the learning process and accessing of resources...
March 2018: Biochemistry and Molecular Biology Education
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