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Biochemistry and Molecular Biology Education

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https://www.readbyqxmd.com/read/30242954/application-of-mini-clinical-evaluation-exercise-for-assessing-the-integrated-based-learning-during-physical-diagnostic-course
#1
Jingjing Da, Yan Ran, Mingjing Pi, Jing Wu, Rong Dong, Qian Li, Qian Zhang, Xiangyan Zhang, Yan Zha
Medical education paradigm has been questioned for the requirements of improving the quality and quantity of medical students. This study was to explore the efficiency of integrated-based learning (IBL) used mini-clinical evaluation exercise (mini-CEX) during physical diagnostics course. One hundred and eleven volunteered students were randomly divided into three groups: lecture-based learning (LBL), case-based learning (CBL), and IBL. Nephrotic syndrome was the teaching content. In the IBL group, students were provided the guideline and additional interpretation from the instructor about the basic knowledge related to disease as vertical integration curriculum...
September 22, 2018: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/30226652/periodic-expression-of-cell-cycle-regulators-a-laboratory-experiment-proposal-for-students-in-molecular-and-cell-biology
#2
Inma Quilis, J Carlos Igual
This article describes a laboratory exercise designed for undergraduate students in the subject of "Regulation of cell proliferation" which allows the students to carry out a research experiment in an important field such as cell cycle control, and to be introduced to a widely used technique in molecular biology laboratories such as the western blot. The cell cycle is regulated by the succession of cyclin-CDK kinase activities. Activation and inactivation of different cyclin-CDK complexes depend on the control of their positive and negative regulators, cyclins and CDK inhibitors (CKIs), respectively...
September 18, 2018: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/30221447/illustration-of-sexual-differentiation-to-undergraduates-with-a-conditional-gene-expression-system
#3
Jie Shen
This work describes the development and evaluation of a two-session laboratory class based on Tet-on technology and sex determination in the fruit fly Drosophila melanogaster. The Tet-on system allows conditional control of gene expression, when doxycycline is applied. A laboratory exercise has been developed to illustrate how the Tet-on technology conditionally over-expresses the key sex determining gene transformer (tra) during development and how to inhibit sex differentiation in males, results in a lack of external genitalia and sex comb size reduction...
September 16, 2018: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/30204283/visualizing-cellular-stress-a-hypothesis-driven-confocal-laboratory-exercise-to-identify-compounds-that-activate-heat-shock-factor-binding-at-hsp70-loci
#4
Annette Choi, Mengqi Wang, Stacy Hrizo, Martin S Buckley
Exposure of organisms to high temperatures and various chemical and physical stressors can cause protein misfolding and aggregation. In turn, this can disrupt the functions of proteins, threatening both development and homeostasis. To overcome this, cells can initiate the highly conserved heat shock (HS) stress response pathway. In eukaryotes, this is a coordinated cellular response, in which the master HS activator, heat shock factor (HSF), is rapidly recruited to the HS protein genes, and triggers the recruitment of additional coactivator proteins that facilitate gene expression...
September 11, 2018: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/30066985/overexpression-immunodetection-and-site-directed-mutagenesis-of-anabaena-sp-pcc-7120-flavodoxin-a-comprehensive-laboratory-practice-on-molecular-biology
#5
Andrés González, María F Fillat
Recombinant protein expression and site-directed mutagenesis of target genes have demonstrated an increasing importance in the fields of molecular biology, biochemistry, biotechnology, and medicine. By using the flavodoxin of the model cyanobacterium Anabaena sp. PCC 7120 as a laboratory tool, we designed a comprehensive laboratory practice encompassing several well-established molecular biology techniques and procedures in order to fulfill two main objectives: (1) overexpression and immunodetection of Anabaena flavodoxin in recombinant Escherichia coli cell extracts, and (2) site-directed mutagenesis of the Anabaena flavodoxin gene isiB...
August 1, 2018: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/29989352/identification-of-an-unknown-glycoprotein-from-whole-cell-lysate-using-cona-and-mass-spectrometry
#6
Jianghong Yan, Shupeng Hu, Xiudan Xu, Dezhang Zhao, Mingjun Wu, Suxin Luo, Yong Xia
Medical laboratory technology major was set up to meet rapid development of science and medical research technology in 2013. Students majoring in medical laboratory had learnt a lot of techniques distributed among different specialized courses. But, they did not understand why they had to learn these techniques and how they were applied in a real-world research setting. In a one-month innovation experimental practice described herein, students had learnt to induce, purify and identify an unknown glycoprotein from whole cell lysate using conA-based affinity chromatography and mass spectrometry...
July 2018: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/29984554/protein-origami-a-program-for-the-creation-of-3d-paper-models-of-folded-peptides
#7
Sabine Reißer, Sebastian Prock, Hartmut Heinzmann, Anne S Ulrich
Protein ORIGAMI (http://ibg.kit.edu/protein_origami) is a browser-based web application that allows the user to create straightforward 3D paper models of folded peptides for research, teaching and presentations. An amino acid sequence can be turned into α-helices, β-strands and random coils that can be printed out and folded into properly scaled models, with a color code denoting the biophysical characteristics of each amino acid residue (hydrophobicity, charge, etc.). These models provide an intuitive visual and tactile understanding of peptide interactions with other partners, such as helix-helix assembly, oligomerization, membrane binding, or pore formation...
July 2018: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/29984537/developing-a-conversation-a-strategy-to-engage-faculty-in-pedagogical-change
#8
Allison M Mercer, Jennifer E Lewis, Susan Sutheimer, Adele J Wolfson
Personal interviews were conducted with biochemistry faculty during which they were presented with student performances on a content survey. From these interviews, four themes that reflect faculty responses to the surveys emerged: awareness of student understanding, self-reflection on teaching practice, planned collaboration with colleagues, and emotional reactions. Here, we discuss these themes and their implications for creating conversation designed to promote reflection on biochemistry teaching. © 2018 by The International Union of Biochemistry and Molecular Biology, 46:382-389, 2018...
July 2018: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/29984456/integrating-cell-and-molecular-biology-concepts-comparing-learning-gains-and-self-efficacy-in-corresponding-live-and-virtual-undergraduate-laboratory-experiences
#9
Lara K Goudsouzian, Patricia Riola, Karen Ruggles, Pranshu Gupta, Michelle A Mondoux
Multiple pedagogical approaches, such as experimental experiences or computer-based activities, have been shown to increase student learning and engagement. We have developed a laboratory module that includes both a traditional "live" experimental component and a student-designed "virtual" computer simulation component. This laboratory employs the mating pathway of Saccharomyces cerevisiae (yeast) to demonstrate four fundamental cell and molecular biology concepts: cell signaling, cytoskeleton, cell cycle, and cell cycle checkpoints...
July 2018: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/29732711/biochemistry-cure-lab-survey
#10
LETTER
Paul A Craig
No abstract text is available yet for this article.
July 2018: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/29732677/creation-and-implementation-of-a-flipped-jigsaw-activity-to-stimulate-interest-in-biochemistry-among-medical-students
#11
Charlene Williams, Susan Perlis, John Gaughan, Sangita Phadtare
Learner-centered pedagogical methods that are based on clinical application of basic science concepts through active learning and problem solving are shown to be effective for improving knowledge retention. As the clinical relevance of biochemistry is not always apparent to health-profession students, effective teaching of medical biochemistry should highlight the implications of biochemical concepts in pathology, minimize memorization, and make the concepts memorable for long-term retention. Here, we report the creation and successful implementation of a flipped jigsaw activity that was developed to stimulate interest in learning biochemistry among medical students...
July 2018: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/29717551/how-to-calculate-thermostability-of-enzymes-using-a-simple-approach
#12
Abdul A N Saqib, Khawar S Siddiqui
Determination of thermostability of enzymes is of prime importance for their successful industrial applications and, yet, the published data has often been incompletely analyzed to assess the suitability of enzymes. It is possible to determine meaningful thermostability parameters from the routinely acquired data through a straightforward method that is not only more informative but also provides a means to compare thermostability of enzymes from different sources. Here, we describe a simple, effective, and economical way to determine enzyme thermostability...
July 2018: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/29717550/an-integrated-course-based-on-two-dimensional-electrophoresis-and-mass-spectrometry-analysis-to-teach-proteomic-techniques-for-undergraduate-students
#13
Shanshan Sha, Liqiu Jia, Yi Xin, Weili Yao, Yufang Ma
Here, we developed an integrated course based on two dimensional-electrophoresis and spectrometry mass (2DE-MS) technique for undergraduate students to help them learn proteomic techniques. The soluble proteins in wild type and gene knockout bacteria were separated by 2DE and the differently expressed proteins were identified by MS analysis. The proteomic data was finally confirmed by RT-PCR detection. The separated experiments of 2DE, MS, RNA isolation, RT-PCR, as well as essential bioinformatic analysis, were integrated into a one-week course, which provided students an opportunity to systematically understand the proteomic techniques and their applications in current scientific research...
July 2018: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/29694708/aqueous-biphasic-systems-in-the-separation-of-food-colorants
#14
João H P M Santos, Emanuel V Capela, Isabel Boal-Palheiros, João A P Coutinho, Mara G Freire, Sónia P M Ventura
Aqueous biphasic systems (ABS) composed of polypropylene glycol and carbohydrates, two benign substances are proposed to separate two food colorants (E122 and E133). ABS are promising extractive platforms, particularly for biomolecules, due to their aqueous and mild nature (pH and temperature), reduced environmental impact and processing costs. Another major aspect considered, particularly useful in downstream processing, is the "tuning" ability for the extraction and purification of these systems by a proper choice of the ABS components...
July 2018: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/29668075/a-comparison-of-debate-and-role-play-in-enhancing-critical-thinking-and-communication-skills-of-medical-students-during-problem-based-learning
#15
Rabia Latif, Sadaf Mumtaz, Rafia Mumtaz, Aamir Hussain
Debate and role play for learning critical thinking and communication skills are being increasingly used in various undergraduate medical schools worldwide. We aim to compare students' views about effectiveness of two teaching strategies; debate and role play to exercise critical thinking and communication skills during problem-based learning (PBL). This is a comparative, cross-sectional, and questionnaire-based study. Our subjects were second year undergraduate female medical students enrolled in Imam Abdulrahman Bin Faisal University (IAU), College of Medicine from September 2014-2016, divided into 10 small PBL groups (10-13 students/group/year)...
July 2018: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/29528559/improving-large-class-performance-and-engagement-through-student-generated-question-banks
#16
Dale Hancock, Nicole Hare, Paul Denny, Gareth Denyer
Disciplines such as Biochemistry and Molecular Biology, which involve concepts not included in the high-school curriculum, are very challenging for many first year university students. These subjects are particularly difficult for students accustomed to surface learning strategies involving memorization and recall of facts, as a deeper understanding of the relationship between concepts is needed for successful transfer to related areas and subsequent study. In this article, we explore an activity in a very large first year Molecular Biology course, in which students create multiple-choice questions related to targeted learning outcomes, and then answer and evaluate one another's questions...
July 2018: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/29528558/preparing-graduate-student-teaching-assistants-in-the-sciences-an-intensive-workshop-focused-on-active-learning
#17
Julie A Roden, Susanne Jakob, Casey Roehrig, Tamara J Brenner
In the past ten years, increasing evidence has demonstrated that scientific teaching and active learning improve student retention and learning gains in the sciences. Graduate teaching assistants (GTAs), who play an important role in undergraduate education at many universities, require training in these methods to encourage implementation, long-term adoption, and advocacy. Here, we describe the design and evaluation of a two-day training workshop for first-year GTAs in the life sciences. This workshop combines instruction in current research and theory supporting teaching science through active learning as well as opportunities for participants to practice teaching and receive feedback from peers and mentors...
July 2018: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/29528553/design-of-a-drug-discovery-course-for-non-science-majors
#18
Danielle H Dube
"Drug Discovery" is a 13-week lecture and laboratory-based course that was developed to introduce non-science majors to foundational chemistry and biochemistry concepts as they relate to the unifying theme of drug discovery. The first part of this course strives to build students' understanding of molecules, their properties, the differences that enable them to be separated from one another, and their abilities to bind to biological receptors and elicit physiological effects. After building students' molecular worldview, the course then focuses on four classes of drugs: antimicrobials, drugs that affect the mind, steroid-based drugs, and anti-cancer drugs...
July 2018: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/29512279/a-course-based-undergraduate-research-experience-investigating-the-consequences-of-nonconserved-mutations-in-lactate-dehydrogenase
#19
Allan Ayella, Moriah R Beck
There is a growing movement to involve undergraduate students in authentic research experiences. A variety of studies have indicated the strength of this approach in developing scientific aptitude, confidence, critical thinking skills, and increasing the likelihood to become career scientists. Course-based undergraduate research experiences (CUREs) foster opportunities for students to carry out authentic research at both primarily undergraduate and large research institutions. Here, we describe a novel CURE-based biochemistry laboratory course to explore the consequences of mutagenesis of non-conserved amino acid sites in the structure and function of the lactate dehydrogenase enzyme and demonstrate how collaborations between institutions can facilitate real research experiences at any type of institution...
May 2018: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/29512269/from-gene-to-structure-lactobacillus-bulgaricus-d-lactate-dehydrogenase-from-yogurt-as-an-integrated-curriculum-model-for-undergraduate-molecular-biology-and-biochemistry-laboratory-courses
#20
Jeffrey A Lawton, Noelle A Prescott, Ping X Lawton
We have developed an integrated, project-oriented curriculum for undergraduate molecular biology and biochemistry laboratory courses spanning two semesters that is organized around the ldhA gene from the yogurt-fermenting bacterium Lactobacillus bulgaricus, which encodes the enzyme d-lactate dehydrogenase. The molecular biology module, which consists of nine experiments carried out over eleven sessions, begins with the isolation of genomic DNA from L. bulgaricus in yogurt and guides students through the process of cloning the ldhA gene into a prokaryotic expression vector, followed by mRNA isolation and characterization of recombinant gene expression levels using RT-PCR...
May 2018: Biochemistry and Molecular Biology Education
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