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BMC Immunology

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https://www.readbyqxmd.com/read/27887569/immune-modulation-of-cd4-cd25-regulatory-t-cells-by-zoledronic-acid
#1
Hsien Liu, Shih-Han Wang, Shin-Cheh Chen, Ching-Ying Chen, Jo-Lin Lo, Tsun-Mei Lin
BACKGROUND: CD4(+)CD25(+) regulatory T (Treg) cells suppress tumor immunity by inhibiting immune cells. Manipulation of Treg cells represents a new strategy for cancer treatment. Zoledronic acid (ZA), a nitrogen-containing bisphosphonate, inhibits the expression of receptor activator of nuclear factor kappa-B ligand (RANKL) on osteoblasts to inhibit osteoclastogenesis. In a mouse model of bisphosphonate-related osteonecrosis of the jaw, administration of ZA suppressed Treg-cell activity and activated inflammatory Th17 cells...
November 25, 2016: BMC Immunology
https://www.readbyqxmd.com/read/27835944/optimization-and-evaluation-of-luminex-performance-with-supernatants-of-antigen-stimulated-peripheral-blood-mononuclear-cells
#2
Mathieu Surenaud, Céline Manier, Laura Richert, Rodolphe Thiébaut, Yves Levy, Sophie Hue, Christine Lacabaratz
BACKGROUND: The Luminex bead-based multiplex assay is useful for quantifying immune mediators such as cytokines and chemokines. Cross-comparisons of reagents for this technique from different suppliers have already been performed using serum or plasma but rarely with supernatants collected from antigen-stimulated peripheral blood mononuclear cells (PBMC). Here, we first describe an optimization protocol for cell culture including quantity of cells and culture duration to obtain reproducible cytokine and chemokine quantifications...
November 11, 2016: BMC Immunology
https://www.readbyqxmd.com/read/27825298/the-ut-family-of-mhc-class-i-loci-unique-to-non-eutherian-mammals-has-limited-polymorphism-and-tissue-specific-patterns-of-expression-in-the-opossum
#3
Katina V Krasnec, Anthony T Papenfuss, Robert D Miller
BACKGROUND: The Major Histocompatibility Complex (MHC) class I family of genes encode for molecules that have well-conserved structures, but have evolved to perform diverse functions. The availability of the gray, short-tailed opossum, Monodelphis domestica whole genome sequence has allowed for analysis of MHC class I gene content in this marsupial. Utilization of a novel method to search for MHC related domain structures revealed a previously unknown family of MHC class I-related genes...
November 8, 2016: BMC Immunology
https://www.readbyqxmd.com/read/27793087/lipoproteins-attenuate-tlr2-and-tlr4-activation-by-bacteria-and-bacterial-ligands-with-differences-in-affinity-and-kinetics
#4
Jeroen van Bergenhenegouwen, Aletta D Kraneveld, Lieke Rutten, Johan Garssen, Arjan P Vos, Anita Hartog
BACKGROUND: The small intestine is a specialized compartment were close interactions take place between host, microbes, food antigens and dietary fatty acids. Dietary fats get absorbed by epithelial cells and processed into a range of lipoprotein particles after which they are basolaterally secreted and collected in the lymphatics. In contrast to the colon, the small intestine is covered only by a thin mucus coat that allows for intimate interactions between host-cells and microbes. Lipoproteins have long been recognized as protective factors in infectious diseases via the neutralization of bacterial toxins like lipopolysaccharides...
October 28, 2016: BMC Immunology
https://www.readbyqxmd.com/read/27784285/inhibition-of-histone-methyltransferase-ezh2-ameliorates-early-acute-renal-allograft-rejection-in-rats
#5
Long Li, Yi Zhang, Ming Xu, Ruiming Rong, Jina Wang, Tongyu Zhu
BACKGROUND: Although histone methyltransferases EZH2 has been proved to have significant regulatory effect on the immune rejection after hematopoietic stem cell transplantation, its role in solid-organ transplantation remains uncovered. In this study, we investigate whether histone methylation regulation can impact renal allograft rejection in rat models. RESULTS: Allogeneic rat renal transplantation model (Wistar to Lewis) was established, and the recipients were administrated with EZH2 inhibitor DZNep after transplantation...
October 26, 2016: BMC Immunology
https://www.readbyqxmd.com/read/27769184/distinct-phenotypic-subpopulations-of-circulating-cd4-cxcr5-follicular-helper-t-cells-in-children-with-active-iga-vasculitis
#6
Deying Liu, Jinxiang Liu, Jinghua Wang, Congcong Liu, Sirui Yang, Yanfang Jiang
BACKGROUND: Circulating follicular helper T (Tfh) cells are a heterogeneous population of CD4(+) helper T cells that promotes pathogenic immune responses in autoimmune diseases. In this study, we examined the status of different subpopulations of Tfh cells in peripheral circulation and their associations with various clinical characteristics of IgA vasculitis (IgAV). METHODS: According to the phenotypic expressions of different molecules, focus was given on six subpopulations of Tfh cells: CD4(+)CXCR5(+), CD4(+)CXCR5(+)ICOS(+), CD4(+)CXCR5(+)ICOS(+)PD-1(+), CD4(+)CXCR5(+)ICOS(high)PD-1(high), CD4(+)CXCR5(+)ICOS(-)PD-1(+), and CXCR5(+)CD45RA(-)IL-21(+)...
October 21, 2016: BMC Immunology
https://www.readbyqxmd.com/read/27756214/chitosan-gel-vaccine-protects-against-tumour-growth-in-an-intracaecal-mouse-model-of-cancer-by-modulating-systemic-immune-responses
#7
Andrew J Highton, Adam Girardin, Georgia M Bell, Sarah M Hook, Roslyn A Kemp
BACKGROUND: Vaccination generating a robust memory population of CD8(+) T cells may provide protection against cancer. However, immune therapies for cancer are influenced by the local tumour immune microenvironment. An infiltrate of T cells into tumours of people with colorectal cancer has proven to be a significant indicator of good prognosis. METHODS: We used an intracaecal mouse model of cancer to determine whether a protective immune response against a mucosal gut tumour could be generated using a systemic intervention...
October 18, 2016: BMC Immunology
https://www.readbyqxmd.com/read/27729009/a-new-high-throughput-sequencing-method-for-determining-diversity-and-similarity-of-t-cell-receptor-tcr-%C3%AE-and-%C3%AE-repertoires-and-identifying-potential-new-invariant-tcr-%C3%AE-chains
#8
Kazutaka Kitaura, Tadasu Shini, Takaji Matsutani, Ryuji Suzuki
BACKGROUND: High-throughput sequencing of T cell receptor (TCR) genes is a powerful tool for analyses of antigen specificity, clonality and diversity of T lymphocytes. Here, we developed a new TCR repertoire analysis method using 454 DNA sequencing technology in combination with an adaptor-ligation mediated polymerase chain reaction (PCR). This method allows the amplification of all TCR genes without PCR bias. To compare gene usage, diversity and similarity of expressed TCR repertoires among individuals, we conducted next-generation sequencing (NGS) of TRA and TRB genes in peripheral blood mononuclear cells from 20 healthy human individuals...
October 11, 2016: BMC Immunology
https://www.readbyqxmd.com/read/27716047/intranasal-immunization-with-recombinant-toxoplasma-gondii-actin-depolymerizing-factor-confers-protective-efficacy-against-toxoplasmosis-in-mice
#9
Zhuanzhuan Liu, Litian Yin, Yaqing Li, Fei Yuan, Xiaofan Zhang, Jiazhi Ma, Hongmei Liu, Yanjuan Wang, Kuiyang Zheng, Jianping Cao
BACKGROUND: Toxoplasma gondii is an opportunistic protozoan closely associated with AIDS and vertical transmission. T. gondii actin depolymerizing factor (TgADF) plays an important role in actin cytoskeleton remodeling, and it is required to invade host cells. TgADF was a promising vaccine candidate. To observe the immunological changes and protective efficacy of recombinant TgADF protein (rTgADF) against T. gondii infection, we optimized the intranasal immunization dose of rTgADF and analyzed the survival rate and tachyzoite loads in mouse tissues after oral challenge with T...
October 6, 2016: BMC Immunology
https://www.readbyqxmd.com/read/27716059/il-23-plasma-level-is-strongly-associated-with-cmv-status-and-reactivation-of-cmv-in-renal-transplant-recipients
#10
Mahmoud Sadeghi, Imad Lahdou, Gerhard Opelz, Arianeb Mehrabi, Martin Zeier, Paul Schnitzler, Volker Daniel
BACKGROUND: Cytomegalovirus seropositivity is an independent risk factor for atherosclerosis in patients with ESRD. Donor CMV seropositivity is associated with higher graft loss. Dendritic cells, macrophages and Th17 lymphocytes are defined as producers of IL-23. IL-23 is thought to be involved in the promotion of Th17 cell polarization. Latent CMV-induced Th17 might be involved in the pathogenesis of CMV infection in patients with ESRD. We aimed to evaluate associations of Th17-dependent cytokines with ESRD, CMV status and post-transplant outcome in kidney transplantation...
October 3, 2016: BMC Immunology
https://www.readbyqxmd.com/read/27716046/immune-analysis-of-expression-of-il-17-relative-ligands-and-their-receptors-in-bladder-cancer-comparison-with-polyp-and-cystitis
#11
Yanbo Liu, Wanguo Yang, Lijing Zhao, Zuowen Liang, Weigao Shen, Qinlong Hou, Zhenjiang Wang, Jing Jiang, Sun Ying
BACKGROUND: Bladder cancer, cystitis and bladder polyp are the most common urinary system diseases all over the world. Our former research results show that IL-17A and IL-17 F contribute to the pathogenesis of benign prostatic hyperplasia (BPH) and prostate cancer (Pca) while IL-17E interacting with IL-17RB might have an anti-tumor effect. RESULTS: Using imunohistochemistry, we systemically compared immunoreactivity of ligands (IL-17A, E and F) and receptors (IL-17RA, IL-17RB and IL-17RC) of IL-17 family, infiltration of inflammatory cells and changes of structural cells (fibroblast cells, smooth muscle and vascular endothelial cells) in sections of bladder tissues from subjects with bladder cancer, cystitis and bladder polyp...
October 3, 2016: BMC Immunology
https://www.readbyqxmd.com/read/27687879/treatment-with-hizentra-in-patients-with-primary-and-secondary-immunodeficiencies-a-real-life-non-interventional-trial
#12
J F Viallard, P Agape, V Barlogis, G Cozon, C Faure, F Fouyssac, C Gaud, M P Gourin, M Hamidou, C Hoarau, F Husseini, M Ojeda-Uribe, M Pavic, I Pellier, A Perlat, N Schleinitz, B Slama
BACKGROUND: Although Hizentra is indicated for immunoglobulin replacement therapy in patients with primary and secondary immunodeficiencies, phase III trials have focused on patients with primary immunodeficiencies. In this 9-month, real-life, prospective, non-interventional, longitudinal, multicenter study of patients with primary and secondary immunodeficiencies in France, treatment modalities (primary endpoint), efficacy, safety, tolerability, quality of life, and treatment satisfaction were evaluated using descriptive statistics...
September 29, 2016: BMC Immunology
https://www.readbyqxmd.com/read/27671862/plasma-fetuin-a-%C3%AE-2-hs-glycoprotein-correlates-negatively-with-inflammatory-cytokines-chemokines-and-activation-biomarkers-in-individuals-with-type-2-diabetes
#13
Sardar Sindhu, Nadeem Akhter, Steve Shenouda, Ajit Wilson, Rasheed Ahmad
BACKGROUND: Fetuin-A/AHSH is a novel hepatokine that acts as a vascular calcification inhibitor and as an endogenous TLR-4 ligand. Fetuin-A may act as a positive or negative acute phase protein (APP) in disease conditions. The relationship between circulatory fetuin-A and inflammatory biomarkers in type-2 diabetes (T2D) remains controversial. Therefore, the purpose of this study was to determine the plasma fetuin-A levels in 53 T2D (BMI = 29.7 ± 4.5 kg/m(2)) and 72 non-diabetic individuals (BMI = 28...
September 26, 2016: BMC Immunology
https://www.readbyqxmd.com/read/27671753/silencing-of-cd47-and-sirp%C3%AE-by-polypurine-reverse-hoogsteen-hairpins-to-promote-mcf-7-breast-cancer-cells-death-by-pma-differentiated-thp-1-cells
#14
Gizem Bener, Alex J Félix, Cristina Sánchez de Diego, Isabel Pascual Fabregat, Carlos J Ciudad, Véronique Noé
BACKGROUND: In the context of tumor immunology, tumor cells have been shown to overexpress CD47, an anti-phagocytic signal directed to macrophages to escape from phagocytosis by interacting with Signal Regulatory Protein α SIRPα. In the present work, we designed Polypurine reverse Hoogsteen hairpins, PPRHs, to silence the expression of CD47 in tumor cells and SIRPα in macrophages with the aim to eliminate tumor cells by macrophages in co-culture experiments. METHODS: THP-1 cells were differentiated to macrophages with PMA...
September 26, 2016: BMC Immunology
https://www.readbyqxmd.com/read/27671547/active-immunization-with-human-interleukin-15-induces-neutralizing-antibodies-in-non-human-primates
#15
Yunier Rodríguez-Álvarez, Yanelys Morera-Díaz, Haydee Gerónimo-Pérez, Jorge Castro-Velazco, Rafael Martínez-Castillo, Pedro Puente-Pérez, Vladimir Besada-Pérez, Eugenio Hardy-Rando, Araceli Chico-Capote, Klaudia Martínez-Cordovez, Alicia Santos-Savio
BACKGROUND: Interleukin-15 is an immunostimulatory cytokine overexpressed in several autoimmune and inflammatory diseases such as Rheumatoid Arthritis, psoriasis and ulcerative colitis; thus, inhibition of IL-15-induced signaling could be clinically beneficial in these disorders. Our approach to neutralize IL-15 consisted in active immunization with structurally modified human IL-15 (mhIL-15) with the aim to induce neutralizing antibodies against native IL-15. In the present study, we characterized the antibody response in Macaca fascicularis, non-human primates that were immunized with a vaccine candidate containing mhIL-15 in Aluminum hydroxide (Alum), Montanide and Incomplete Freund's Adjuvant...
September 26, 2016: BMC Immunology
https://www.readbyqxmd.com/read/27669687/an-effective-cytokine-adjuvant-vaccine-induces-autologous-t-cell-response-against-colon-cancer-in-an-animal-model
#16
Huanyu Ju, Wenjing Xing, Jinfeng Yang, Yang Zheng, Xiuzhi Jia, Benning Zhang, Huan Ren
BACKGROUND: Despite recent advances in early detection and improvements in chemotherapy for colon cancer, the patients still face poor prognosis of postoperative recurrence and metastasis, the median survival for patients with metastatic colorectal cancer is approximately 22-24 months. Some immunotherapeutic approaches had been attempted in colon cancer patients to significantly increase overall survival. A vaccine based approach has shown a novel direction for colon cancer prevention and therapy...
September 26, 2016: BMC Immunology
https://www.readbyqxmd.com/read/26979060/the-effects-of-storage-temperature-on-pbmc-gene-expression
#17
Jun Yang, Norma Diaz, Joseph Adelsberger, Xueyuan Zhou, Randy Stevens, Adam Rupert, Julia A Metcalf, Mike Baseler, Christine Barbon, Tomozumi Imamichi, Richard Lempicki, Louis M Cosentino
BACKGROUND: Cryopreservation of peripheral blood mononuclear cells (PBMCs) is a common and essential practice in conducting research. There are different reports in the literature as to whether cryopreserved PBMCs need to only be stored ≤ -150 °C or can be stored for a specified time at -80 °C. Therefore, we performed gene expression analysis on cryopreserved PBMCs stored at both temperatures for 14 months and PBMCs that underwent temperature cycling 104 times between these 2 storage temperatures...
March 15, 2016: BMC Immunology
https://www.readbyqxmd.com/read/26970734/tirf-imaging-of-fc-gamma-receptor-microclusters-dynamics-and-signaling-on-macrophages-during-frustrated-phagocytosis
#18
Jia Lin, Svetlana Kurilova, Brandon L Scott, Elizabeth Bosworth, Bradley E Iverson, Elizabeth M Bailey, Adam D Hoppe
BACKGROUND: Recent evidence indicates that in addition to the T-cell receptor, microclustering is an important mechanism for the activation of the B-cell receptor and the mast cell Fcε-receptor. In macrophages and neutrophils, particles opsonized with immunoglobulin G (IgG) antibodies activate the phagocytic Fcγ-receptor (FcγR) leading to rearrangements of the actin cytoskeleton. The purpose of this study was to establish a system for high-resolution imaging of FcγR microclustering dynamics and the recruitment of the downstream signaling machinery to these microclusters...
March 12, 2016: BMC Immunology
https://www.readbyqxmd.com/read/27645787/repeated-cycles-of-5-fluorouracil-chemotherapy-impaired-anti-tumor-functions-of-cytotoxic-t-cells-in-a-ct26-tumor-bearing-mouse-model
#19
Yanhong Wu, Zhenling Deng, Huiru Wang, Wenbo Ma, Chunxia Zhou, Shuren Zhang
BACKGROUND: Recently, the immunostimulatory roles of chemotherapeutics have been increasingly revealed, although bone marrow suppression is still a common toxicity of chemotherapy. While the numbers and ratios of different immune subpopulations are analyzed after chemotherapy, changes to immune status after each cycle of treatment are less studied and remain unclear. RESULTS: To determine the tumor-specific immune status and functions after different cycles of chemotherapy, we treated CT26 tumor-bearing mice with one to four cycles of 5-fluorouracil (5-FU)...
2016: BMC Immunology
https://www.readbyqxmd.com/read/27577610/multi-layered-gag-specific-immunodominant-responses-contribute-to-improved-viral-control-in-the-crf01_ae-subtype-of-hiv-1-infected-msm-subjects
#20
Fanming Jiang, Xiaoxu Han, Hui Zhang, Bin Zhao, Minghui An, Junjie Xu, Zhenxing Chu, Tao Dong, Hong Shang
BACKGROUND: The purpose of this study was to characterize specific cytotoxic T-cell (CTL) responses in men who have sex with men (MSM) subjects infected with the human immunodeficiency virus type 1 (HIV-1) CRF01_AE subtype during the first year of infection and impacts on viral control and evolution. RESULTS: Fifteen HIV-1 primary infected cases were recruited from Liaoning MSM prospective cohort. CTL responses to Gag, Pol and Nef proteins at 3 month and 1 year post infection were detected with Gamma interferon enzyme-linked immunospot (ELISPOT) assay using optimized consensus overlapping peptides, as well as the viral quasispecies sequences from the synchronous plasma...
2016: BMC Immunology
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