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Reproduction: the Official Journal of the Society for the Study of Fertility

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https://www.readbyqxmd.com/read/28630101/oviduct-extracellular-vesicles-protein-content-and-their-role-during-oviduct-embryo-cross-talk
#1
Carmen Alminana, Emilie Corbin, Guillaume Tsikis, Agostinho S Alcantara-Neto, Valérie Labas, Karine Reynaud, Laurent Galio, Rustem Uzbekov, Anastasiia S Garanina, Xavier Druart, Pascal Mermillod
Successful pregnancy requires an appropriate communication between the mother and the embryo. Recently, exosomes and microvesicles, both membrane-bound extracellular vesicles (EVs) present in the oviduct fluid have been proposed as key modulators of this unique cross-talk. However, little is known about their content and their role during oviduct-embryo dialogue. Given the known differences in secretions by in vivo and in vitro oviduct epithelial cells (OEC), we aimed at deciphering the oviduct EVs protein content from both sources...
June 19, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28630100/nlrp2-and-faf1-deficiency-blocks-early-embryogenesis-in-the-mouse
#2
Hui Peng, Hai-Jun Liu, Fang Liu, Yuyun Gao, Jing Chen, Jianchao Huo, Jinglin Han, Tianfang Xiao, Wenchang Zhang
Nlrp2 is a maternal effect gene specifically expressed by mouse ovaries; deletion of this gene from zygotes is known to result in early embryonic arrest. In the present study, we identified FAF1 protein as a specific binding partner of the NLRP2 protein in both mouse oocytes and preimplantation embryos. In addition to early embryos, both Faf1 mRNA and protein were detected in multiple tissues. NLRP2 and FAF1 proteins were co-localized to both the cytoplasm and nucleus during the development of oocytes and preimplantation embryos...
June 19, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28630099/kndy-neurone-activation-prior-to-the-lh-surge-of-the-ewe-is-disrupted-by-lps
#3
Chrysanthi Fergani, Jean Routly, David Jones, Lucy Pickavance, Robert Frank Smith, Hilary Dobson
In the ewe, steroid hormones act on the hypothalamic arcuate nucleus (ARC) to initiate the GnRH/LH surge. Within the ARC, steroid signal transduction may be mediated by dopamine, β-endorphin or neuropeptide Y (NPY) expressing cells, as well as those co-localising kisspeptin, neurokinin B (NKB), and dynorphin (termed KNDy). We investigated the time during the follicular phase when these cells become activated (i.e., co-localise c-Fos) relative to the timing of the LH surge onset and may, therefore, be involved in the surge generating mechanism...
June 19, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28630098/comparative-micrornaome-analysis-of-the-testis-and-ovary-of-the-chinese-giant-salamander
#4
Rui Chen, Jian Du, Lin Ma, Li-Qing Wang, Sheng-Song Xie, Chang-Ming Yang, Xian-Yong Lan, Chuan-Ying Pan, Wu-Zi Dong
MicroRNAs (miRNAs) are 18-24 nucleotides non-coding RNAs that regulate gene expression by post-transcriptional suppression of mRNA. The Chinese giant salamander (CGS, Andrias davidianus), which is an endangered species, has become one of important models of animal evolution; however, no miRNA studies on this species have been conducted. In this study, two small RNA libraries of CGS ovary and testis were constructed using deep sequencing technology. A bioinformatics pipeline was developed to distinguish miRNA sequences from other classes of small RNAs represented in the sequencing data...
June 19, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28611113/active-paraoxonase-1-is-synthesised-throughout-the-internal-boar-genital-organs
#5
Isabel Barranco, Cristina Perez-Patiño, Asta Tvarijonaviciute, Inmaculada Parrilla, Alejandro Vicente-Carrillo, Manuel Alvarez-Rodriguez, Jose J Ceron, Emilio Martinez, Heriberto Rodriguez-Martinez, Jordi Roca
The paraoxonase type 1 (PON1) is an enzyme with antioxidant properties recently identified in the seminal plasma (SP) of several species, including the porcine. The aims of the present study were to (1) describe the inmunohistochemical localization of PON1 in the genital organs of fertile boars and (2) evaluate the relationship among PON1 activity and high-density lipoprotein cholesterol (HDL-C) concentration in fluids of the boar genital organs. Immunohistochemical analysis demonstrated that PON1 was present in testis (specifically in Leydig cells, blood vessels, spermatogonia and elongated spermatids), epididymis (specifically in the cytoplasm of the principal epithelial cells, luminal secretion and in the surrounding smooth muscle) and the lining epithelia of the accessory sexual glands (cytoplasmic location in the prostate and membranous in the seminal vesicle and bulbourethral glands)...
June 13, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28611112/microrna-150-regulates-steroidogenesis-of-mouse-testicular-leydig-cells-by-targeting-star
#6
Xu-Jing Geng, Dong-Mei Zhao, Gen-Hong Mao, Li Tan
Leydig cells are essential for male reproductive development throughout life. Production of androgens as well as intermediate steroids is tightly regulated. Although microRNAs (miRNAs) are suggested to play important roles in spermatogenesis, little is currently known regarding the regulation of steroidogenesis by miRNAs in Leydig cells. Here, we found that miR-150 was predominantly expressed in Leydig cells within mouse testis. Therefore, we determined steroidogenesis of the Leydig cells in which miR-150 was knocked down or overexpressed using miR-150 antagomir and agomir, respectively...
June 13, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28592665/signs-of-embryo-maternal-communication-mirnas-in-the-maternal-serum-of-pregnant-pigs
#7
Zaneta Paulina Reliszko, Zdzislaw Gajewski, Monika Marzena Kaczmarek
Circulating miRNAs were proposed to be indicators of normal or complicated pregnancies. Based on this knowledge and our recent transcriptomic approach showing expression of miRNAs in the porcine endometrium, conceptuses and uterine extracellular vesicles during pregnancy, we have hypothesized that signs of ongoing local embryo-maternal crosstalk involving miRNAs can be detected in the circulation of pregnant gilts as early as a few days after maternal recognition of pregnancy. By applying several molecular biology techniques that differ in dynamic range and precision in maternal serum of Day 16 pregnant pigs, we were able to show for the first time increased levels of several miRNAs, previously reported to be expressed in either conceptuses and extracellular vesicles (miR-26a and miR-125b) or pregnant endometrium (miR-23b)...
June 7, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28592664/altered-retinoid-signaling-compromises-decidualization-in-endometriotic-stromal-cells
#8
Mary Ellen Pavone, Saurabh Malpani, Matthew Dyson, Serdar Bulun
Decidualization alters multiple molecular pathways in endometrium to permit successful embryo implantation. We have reported that paracrine factors, including retinoids, secreted from progesterone treated endometrial stromal cells act on nearby epithelial cells to induce the estradiol metabolizing enzyme HSD17B2. This same induction is not seen in endometriotic stromal cells. We have also shown significant differences in retinoid uptake, metabolism and action in endometriotic tissue and stromal cells compared to normal endometrium...
June 7, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28576919/trivial-role-for-nsmce2-during-in-vitro-proliferation-and-differentiation-of-gs-cells
#9
Yi Zheng, Aldo Jongejan, Callista L Mulder, Sebastiaan Mastenbroek, Sjoerd Repping, Yinghua Wang, Jinsong Li, Geert Hamer
Spermatogenesis, starting with spermatogonial differentiation, is characterized by ongoing and dramatic alterations in composition and function of chromatin. Failure to maintain proper chromatin dynamics during spermatogenesis may lead to mutations, chromosomal aberrations or aneuploidies. When transmitted to the offspring, these can cause infertility or congenital malformations. The structural maintenance of chromosomes (SMC) 5/6 protein complex has recently been described to function in chromatin modeling and genomic integrity maintenance during spermatogonial differentiation and meiosis...
June 2, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28559473/paracetamol-acetaminophen-impairs-brain-masculinisation
#10
Anders Hay-Schmidt, Olivia Finkielman, Benjamin Jensen, Christine Høgsbro, Jacob Bak Holm, Kristoffer Haurum Johansen, Tina Kold Jensen, Anderson Martino Andrade, Shanna Swan, Carl-Gustaf Bornehag, Søren Brunak, Bernard Jegou, Karsten Kristiansen, David Møbjerg Kristensen
Paracetamol/acetaminophen (N-Acetyl-p-Aminophenol; APAP) is the preferred analgesic for pain relief and fever during pregnancy. It has therefore caused concern that several studies have reported that prenatal exposure to APAP results in developmental alterations in both the reproductive tract and the brain. Genitals and nervous system of male mammals are actively masculinized during foetal development and early postnatal life by the combined actions of prostaglandins and androgens, resulting in the male-typical reproductive behaviour seen in adulthood...
May 30, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28495852/high-throughput-mrna-sequencing-of-stromal-cells-from-endometriomas-and-endometrium
#11
Kadri Rekker, Merli Saare, Elo Eriste, Tõnis Tasa, Viktorija Kukuškina, Anne Mari Roost, Kristi Anderson, Külli Samuel, Helle Karro, Andres Salumets, Maire Peters
The aetiology of endometriosis is still unclear and to find mechanisms behind the disease development it is important to study each cell type from endometrium and ectopic lesions independently. The objective of this study was to uncover complete mRNA profiles in uncultured stromal cells from paired samples of endometriomas and eutopic endometrium. High-throughput mRNA sequencing revealed over 1,300 dysregulated genes in stromal cells from ectopic lesions, including several novel genes in the context of endometriosis...
May 11, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28495851/different-macrophages-equally-induce-emt-in-endometria-of-adenomyosis-and-normal
#12
Min An, Dong Li, Ming Yuan, Qiuju Li, Lu Zhang, Guo Yun Wang
Endometrial cells and microenvironment are two important factors in the pathogenesis of adenomyosis. Our previous study demonstrated that macrophages can induce eutopic epithelial cells of adenomyosis suffering from epithelial-mesenchymal transition (EMT). The aim of this study is to detect whether macrophages interacting with epithelial cells equally induce the EMT process in normal and eutopic endometria of healthy and adenomyotic patients; and macrophages parallelly polarize to M2. We investigated expressions of epithelial cadherin (E-cadherin), neural cadherin (N-cadherin), cytokeratin7 (CK7), vimentin, transforming growth factor-β1 (TGFB1), SMAD3 and pSMAD3 using Immunohistochemistry and Western Blot, then estimated genetic levels of CD163, IL10 and MMP12 using real-time quantitative polymerase chain reaction (RT-PCR) in macrophages...
May 11, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28468839/human-relaxins-rlnh1-rlnh2-their-receptor-rxfp1-and-fetoplacental-growth
#13
Kelly Yamasato, Pai-Jong Stacy Tsai, James Davis, Sandra Yamamoto, Gillian Bryant-Greenwood
Relaxin, a systemic and placental hormone, has potential roles in fetoplacental growth. Human placenta expresses two RLN genes, RLNH1 and RLNH2. Maternal obesity is common and is associated with abnormal fetal growth. Our aims were to relate systemic and cord blood RLNH2, placental RLNs and their receptor (RXFP1) with fetoplacental growth in context of maternal body mass index, and associations with insulin-like growth factor 2 (IGF2) and vascular endothelial growth factor A (VEGFA) in the same placentas. Systemic, cord blood and placental samples were collected prior to term labor, divided by prepregnancy body mass index: underweight/normal (N=25) and overweight/obese (N=44)...
May 3, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28450315/mirna-mrna-network-involved-in-folliculogenesis-interactome-systems-biology-approach
#14
Abolfazl Bahrami, Seyed Reza Miraie-Ashtiani, Mostafa Sadeghi, Ali Najafi
At later phases of folliculogenesis, the mammalian ovarian follicle contains layers of granulosa cells surrounding an antral cavity. To better understand the molecular basis of follicular growth and granulosa cell maturation, we study transcriptome profiling of granulosa cells from small (< 5 mm) and large (> 10 mm) bovine follicles using simultaneous method of Affymetrix microarrays (24,128 probe sets) and RNA-Seq data sets. In this study proposes a computational method to discover the functional miRNA-mRNA regulatory modules, that is, groups of miRNAs and their target mRNAs that are believed to take part cooperatively in post-transcriptional gene regulation under specific conditions...
April 27, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28432091/suppressive-effects-of-tnf%C3%AE-il6-and-macrophages-on-thecal-androgen-production
#15
Moafaq S Samir, Claire Glister, Dareen Mattar, Mhairi Laird, Philip G Knight
Pro-inflammatory cytokines secreted by macrophages and other cell-types are implicated as intra-ovarian factors affecting different aspects of ovarian function including follicle and corpus luteum 'turnover', steroidogenesis and angiogenesis. Here, we compared granulosal (GC) and thecal (TC) expression of TNF, IL6 and their receptors (TNFR1, TNFRSF1B, IL6R) during bovine antral follicle development; all five mRNA transcripts were detected in both GC and TC and statistically significant cell-type and follicle stage-related differences were evident...
April 21, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28420801/the-impact-of-laparoscopic-ovarian-drilling-on-amh-ovarian-reserve-a-meta-analysis
#16
Saad Aks Amer, Tarek T El Shamy, Cathryn James, Ali H Yosef, Ahmed Aboelfadle Mohamed
Laparoscopic ovarian drilling (LOD) has been widely utilised as an effective treatment in anovulatory women with polycystic ovarian syndrome (PCOS). However, there has been a growing concern over a possible damaging effect of this procedure on ovarian reserve. The objective of this study was to investigate the hypothesis that LOD compromises ovarian reserve as measured by post-operative changes in circulating anti-Müllerian hormone (AMH). This meta-analysis included all cohort studies as well as randomised controlled trials investigating serum AMH concentrations and other ovarian reserve markers in PCOS women undergoing LOD...
April 18, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28420799/neonatal-hypothyroidism-does-not-increase-sertoli-cell-proliferation-in-inos-mice
#17
Nathalia de Lima E Martins Lara, Luiz Renato de França
Sertoli cell (SC) proliferation in mice occurs until two weeks after birth and is mainly regulated by FSH and thyroid hormones. Previous studies have shown that transient neonatal hypothyroidism in laboratory rodents is able to extend SC mitotic activity, leading ultimately to higher testis size and daily sperm production (DSP) in adult animals. Moreover, we have shown that due to higher SC proliferation and lower germ cell apoptosis, iNOS deficiency in mice also results in higher testis size and DSP. Although the cell size was smaller, the Leydig cells (LC) number per testis also significantly increased in iNOS(-/-) mice...
April 18, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28408706/embryo-culture-in-presence-of-oviductal-fluid-induces-dna-methylation-changes-in-bovine-blastocysts
#18
Antonio Daniel Barrera, Elina Vanesa García, Meriem Hamdi, Maria-Jesús Sánchez Calabuig, Angela Patricia Lopez-Cardona, Noelia Fonseca Balvís, Dimitrios Rizos, Alfonso Gutierrez-Adan
During the transit through the oviduct, the early embryo initiates an extensive DNA methylation reprogramming of its genome. Given that these epigenetic modifications are susceptible to environmental factors, components present in the oviductal milieu could affect the DNA methylation marks of the developing embryo. The aim of this study was to examine if culture of bovine embryos with oviductal fluid (OF) can induce DNA methylation changes at specific genomic regions in the resulting blastocysts. In vitro produced zygotes were cultured in medium with 3 mg/mL bovine serum albumin (BSA) or 1...
April 13, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28652254/oviductal-microvesicles-and-their-effect-on-in-vitro-maturation-of-canine-oocytes
#19
Anna Lange-Consiglio, Claudia Perrini, Giulia Albini, Silvia Modina, Valentina Lodde, Eleonora Orsini, Paola Esposti, Fausto Cremonesi
The effect of conditioned medium (CM) or microvesicles (MVs), secreted by multicellular spheroids of oviductal cells, and the involvement of some microRNAs (miRNAs) were investigated in canine oocyte maturation. To generate CM, spheroids were cultured for 3 days. MVs were obtained by ultracentrifugation of CM at 100,000 g and measured for size and concentration by NanoSight instrument. Cumulus-oocyte complexes (COCs) were matured at 38.5°C with 5% CO2 and 5% of O2 in synthetic oviductal fluid (SOF) in biphasic systems: for 24 h, with 5...
August 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28624768/a-revised-asingle-model-to-explain-stem-cell-dynamics-in-the-mouse-male-germline
#20
REVIEW
Tessa Lord, Jon M Oatley
Spermatogonial stem cells (SSCs) and progenitor spermatogonia encompass the undifferentiated spermatogonial pool in mammalian testes. In rodents, this population is comprised of Asingle, Apaired and chains of 4-16 Aaligned spermatogonia. Although traditional models propose that the entire Asingle pool represents SSCs, and formation of an Apaired syncytium symbolizes irreversible entry to a progenitor state destined for differentiation; recent models have emerged that suggest that the Asingle pool is heterogeneous, and Apaired/Aaligned can fragment to produce new SSCs...
August 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
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