journal
MENU ▼
Read by QxMD icon Read
search

Reproduction: the Official Journal of the Society for the Study of Fertility

journal
https://www.readbyqxmd.com/read/29930176/cftr-is-required-for-the-migration-of-primordial-germ-cells-during-zebrafish-early-embryogenesis
#1
Huijuan Liao, Yan Chen, Li Yulong, Shaolong Xue, Mingfeng Liu, Ziyuan Lin, Yanyan Liu, Hsiao Chang Chan, Xiaohu Zhang, Huaqin Sun
Mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene affect fertility in both sexes. However, the involvement of CFTR in regulating germ cell development remains largely unknown. Here, we used zebrafish model to investigate the role of CFTR in primordial germ cells (PGCs) development. We generated a cftr frameshift mutant zebrafish line using CRISPR/Cas9 technique and investigated the migration of PGCs during early embryo development. Our results showed that loss of Cftr impairs the migration of PGCs from dome stages onwards...
June 21, 2018: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/29921626/activated-farnesoid-x-receptor-fxr-expressed-in-human-sperm-alters-its-fertilizing-ability
#2
Rocco Malivindi, Marta Santoro, Daniela De Rose, Salvatore Panza, Serena Gervasi, Vittoria Rago, Saveria Aquila
The farnesoid X receptor alpha (FXR) is a bile acid sensor activated by binding to endogenous bile acids including chenodeoxycholic acid (CDCA). Although, FXR is expressed in male reproductive tissue, the relevance of the receptor on reproduction is scarcely known. Here we demonstrated the FXR presence and its action on several human sperm features. Western blot and Immunofluorescence assays evidenced the FXR expression in human spermatozoa and the localization in the middle piece. CDCA increasing concentrations and GW4064, synthetic ligand of FXR, were used to study the FXR influence on sperm motility, survival, capacitation, acrosome reaction and on glucose as well as lipid metabolism...
June 19, 2018: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/29921625/genomic-integrity-in-the-male-germ-line-evidence-in-support-of-the-disposable-soma-hypothesis
#3
Miguel J Xavier, Lisa A Mitchell, Kristen E McEwan, Rodney Scott, Robert John Aitken
The Big Blue® λSelect-cII selection system has been employed along with whole-exome sequencing, to examine the susceptibility of the male germ line to mutation in two challenging situations: (i) exposure to a chemotherapeutic regime including bleomycin, etoposide and cis-platinum (BEP) and (ii) the ageing process. A 3-week exposure to BEP induced complete azoospermia associated with a loss of developing germ cells and extensive vacuolization of Sertoli cell cytoplasm. Following cessation of treatment, spermatozoa first appeared in the caput epididymis after 6 weeks and by 12 weeks motile spermatozoa could be recovered from the cauda, although the count (P < 0...
June 19, 2018: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/29907662/expression-of-atresia-biomarkers-in-granulosa-cells-after-ovarian-stimulation-in-heifers
#4
David A Landry, Lia Rossi-Perazza, Simon Lafontaine, Marc-Andre Sirard
The use of younger gamete donors in dairy cattle genetic selection programs significantly accelerates genetic gains by decreasing the interval between generations. Ovarian stimulation and the practice of follicle-stimulating hormone (FSH) withdrawal, also known as coasting, are intensively used in pre-pubertal heifers without detrimental effects on subsequent reproductive performance but generally with lower embryo yields. However, recent data from embryo transfer programs showed similar embryo yields in younger and sexually mature animals but with a significant difference in the coasting period...
June 15, 2018: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/29907661/nlrp3-in-somatic-non-immune-cells-of-rodent-and-primate-testes
#5
Lena Walenta, Nina Schmid, J U Schwarzer, Frank-Michael Köhn, Henryk Urbanski, Ruediger Behr, Leena Strauss, Matti Poutanen, Artur Mayerhofer
NLRP3 is part of the NLRP3 inflammasome and a global sensor of cellular damage. It was recently discovered in rodent Sertoli cells. We investigated NLRP3 in mouse, human and non-human primate (marmoset and rhesus macaque) testes, employing immunohistochemistry. Sertoli cells of all species expressed NLRP3, and the expression preceded puberty. In addition, peritubular cells of the adult human testes expressed NLRP3. NLRP3 and associated genes (ASC, CASP1, IL1B) were also found in isolated human testicular peritubular cells (HTPCs) and the mouse Sertoli cell line TM4...
June 15, 2018: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/29898906/-designer-babies-almost-thirty-years-on
#6
Alan H Handyside
The first pregnancies and live births following IVF and preimplantation genetic testing (PGT), formerly known as preimplantation genetic diagnosis (PGD), were reported in 1990, almost 30 years ago, in several couples at risk of X-linked inherited conditions, which typically only affect boys inheriting the X chromosome with the affected gene from their carrier mothers. At that time, it was only possible to identify the sex of the embryo by amplifying a Y-linked repeat sequence in single cells biopsied at cleavage stages and avoid the transfer of males, half of which would be affected...
June 13, 2018: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/29891616/contractility-of-the-epididymal-duct-function-regulation-and-potential-drug-effects
#7
Vera Elfgen, Andrea Mietens, Marco Mewe, Thorben Hau, Ralf Middendorff
During their transit through the epididymis, spermatozoa mature and acquire motility and fertilizing capacity. The smooth muscle cells (SMCs) of the epididymal duct are thought to be responsible for the adequate transport of spermatozoa. Thus, precise regulation of SMC function also represents a prerequisite for sperm maturation thereby contributing to male fertility. In this review we would like to highlight various aspects of epididymal SMC function and discuss several angles with respect to regulation of contraction and relaxation...
June 11, 2018: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/29880719/spen-protein-expression-and-interactions-with-chromatin-in-mouse-testicular-cells
#8
Joanna Korfanty, Tomasz Stokowy, Marek Chadalski, Agnieszka Toma-Jonik, Natalia Vydra, Piotr Widlak, Bartosz Wojtaś, Bartłomiej Gielniewski, Wieslawa Widlak
SPEN (spen family transcription repressor) is a nucleic acid-binding protein putatively involved in repression of gene expression. We hypothesized that SPEN could be involved in general down-regulation of the transcription during the heat shock response in mouse spermatogenic cells through its interactions with chromatin. We documented predominant nuclear localization of the SPEN protein in spermatocytes and round spermatids, which was retained after heat shock. Moreover, the protein was excluded from the highly condensed chromatin...
June 7, 2018: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/29880718/regulation-of-gnrh-pulsatility-in-sheep
#9
Casey C Nestor, Michelle N Bedenbaugh, Stanley M Hileman, Lique M Coolen, Michael N Lehman, Robert L Goodman
Early work in ewes provided a wealth of information on the physiological regulation of pulsatile GnRH secretion by internal and external inputs. Identification of the neural systems involved, however, was limited by the lack of information on neural mechanisms underlying generation of GnRH pulses. Over the last decade considerable evidence supported the hypothesis that a group of neurons in the arcuate nucleus that contain kisspeptin, neurokinin B, and dynorphin (KNDy neurons) are responsible for synchronizing secretion of GnRH during each pulse in ewes...
June 7, 2018: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/29875308/impact-of-exercise-on-oocyte-quality-in-the-polg-mitochondrial-dna-mutator-mouse
#10
Christine Faraci, Sofia Annis, Joyce Jin, Housaiyin Li, Konstantin Khrapko, Dori Woods
The mitochondrial DNA (mtDNA) 'mutator' mouse, also called the 'POLG' mouse, is a well-characterized model frequently used for studies of progeroid aging. Harboring a mutation in the proofreading domain of the mitochondrial polymerase, polymerase-γ (POLG), POLG mice acquire mtDNA mutations at an accelerated rate. This results in premature mitochondrial dysfunction and a systemic aging phenotype. Previous work has demonstrated that the progeroid phenotype in POLG is attenuated following endurance exercise, the only reported intervention to extend healthspan and lifespan of these mice...
June 6, 2018: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/29866768/involvement-of-fibroblast-growth-factor-2-fgf2-and-its-receptors-in-the-regulation-of-mouse-sperm-physiology
#11
Lucia Saucedo, Cristian Sobarzo, Nicolás Brukman, Hector Alejandro Guidobaldi, Livia Lustig, Laura Cecilia Giojalas, Mariano Gabriel Buffone, Monica Hebe Vazquez-Levin, Clara Marín-Briggiler
Fibroblast Growth Factor 2 (FGF2) and its receptors (FGFRs) have been described in several tissues, where they regulate cellular proliferation, differentiation, motility and apoptosis. Although FGF2/FGFRs expression in the male reproductive tract has been reported, there is scarce evidence on their presence in the female reproductive tract and their involvement in the modulation of sperm function. Therefore, the objective of this study was to determine the expression of FGF2 in the female reproductive tract and to assess the role of the FGF2/FGFRs system in the regulation of sperm physiology using the murine model...
June 4, 2018: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/29866767/progressive-methylation-of-pou5f1-regulatory-regions-during-blastocyst-development
#12
Eugénie Canon, Luc Jouneau, Thierry Blachère, Nathalie Peynot, Nathalie Daniel, Laurent Boulanger, Linda Maulny, Catherine Archilla, Sarah Voisin, Alice Jouneau, Murielle Godet, Veronique Duranthon
The POU5F1 gene encodes one of the "core" transcription factors necessary to establish and maintain pluripotency in mammals. Its function depends on its precise level of expression, so its transcription has to be tightly regulated. To date, few conserved functional elements have been identified in its 5' regulatory region: a distal and a proximal enhancer, and a minimal promoter, epigenetic modifications of which interfere with POU5F1 expression and function in in vitro-derived cell lines. Also, its permanent inactivation in differentiated cells depends on de novo methylation of its promoter...
June 4, 2018: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/29844226/peri-ovarian-adipose-tissue-contributes-to-intraovarian-control-during-folliculogenesis-in-mice
#13
Liuhong Yang, Lei Chen, Xiaosheng Lu, Anni Tan, Yao Chen, Yalan Li, Xuemei Peng, Shaochun Yuan, Dongqing Cai, Yanhong Yu
Peri-ovarian adipose tissue (POAT) is a kind of intra-abdominal white adipose tissue that is present surrounding the ovaries in rodents. Recent studies demonstrated that POAT-deficient mice displayed a phenotype of delayed antral follicular development, for which decreases in serum estrogen, serum FSH, and FSHR levels were responsible. However, folliculogenesis is regulated by endocrine signals and also modulated by a number of locally produced intraovarian factors whose acts are both autocrine and paracrine...
May 29, 2018: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/29844225/effects-of-micronutrients-on-placental-function-evidence-from-clinical-studies-to-animal-models
#14
Bernadette Carolyn Baker, Dexter Hayes, Rebecca Lee Jones
Micronutrient deficiencies are common in pregnant women due to low dietary intake and increased requirements for fetal development. Low maternal micronutrient status is associated with a range of pregnancy pathologies involving placental dysfunction, including fetal growth restriction (FGR), small for gestational age (SGA), pre-eclampsia and preterm birth. However, clinical trials commonly fail to convincingly demonstrate beneficial effects of supplementation of individual micronutrients, attributed to heterogeneity and insufficient power, potential interactions and lack of mechanistic knowledge of effects on the placenta...
May 29, 2018: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/29794024/reproductive-performance-of-male-mice-after-hypothalamic-ghrelin-administration
#15
Maria Belen Poretti, Camila Frautschi, Eugenia Mercedes Luque, Santiago Bianconi, Ana Carolina Martini, Graciela Stutz, Laura Maria Vincenti, María Emilia Santillán, Marina Flavia Ponzio, Helgi Schiöth, Marta Haydee Fiol De Cuneo, Valeria Paola Carlini
It has been demonstrated that food intake and reproductive physiology are both simultaneously modulated to optimize reproductive success under fluctuating metabolic conditions. Ghrelin (Ghr) is an orexigenic peptide identified as the endogenous ligand of the growth hormone secretagogue receptor that is being investigated for its potential role on reproduction. Considering that data available so far are still limited and characterization of Ghr action mechanism on the reproductive system has not been fully elucidated, we studied the hypothalamus participation in Ghr effects on sperm functional activity, plasma levels of gonodotropins and histological morphology in mice testes after hypothalamic infusion of 0...
May 23, 2018: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/29794023/regulation-of-the-porcine-corpus-luteum-during-pregnancy
#16
Adam Janusz Ziecik, Emilia Przygrodzka, Beenu Moza Jalali, Monika Marzena Kaczmarek
The new corpora lutea (CLs) in pigs are formed from the preovulatory follicles after the luteinizing hormone (LH) surge. However, total autonomy and independence of CLs from LH up to day 12 of cycle has recently been questioned. Transformation of estrous cycle CL to CL of pregnancy initiated by embryonic signals requires not only the cessation of prostaglandin F2 (PGF2) supply to the luteal tissue but also needs the CL to overcome luteolytic acquisition and/or changing its sensitivity to PGF2α during Days 12-14 of pregnancy...
May 23, 2018: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/29789442/ovine-prenatal-growth-restriction-impacts-glucose-metabolism-and-body-composition-throughout-life-in-both-sexes
#17
Jacqueline M Wallace, John S Milne, Raymond P Aitken, Graham W Horgan, Clare L Adam
Low birthweight is a risk factor for later adverse health. Here the impact of placentally-mediated prenatal growth-restriction followed by postnatal nutrient abundance on growth, glucose metabolism and body composition was assessed in both sexes at key stages from birth to mid-adult life. Singleton-bearing adolescent dams were fed control or high nutrient intakes to induce normal or growth-restricted pregnancies, respectively. Restricted lambs had ~40% reduced birthweight. Fractional growth rates were higher in restricted lambs of both sexes predominantly during suckling/juvenile phases...
May 22, 2018: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/29789441/metformin-counteracts-the-effects-of-fsh-on-rat-sertoli-cell-proliferation
#18
Gustavo Marcelo Rindone, Agostina Gorga, Mariana Regueira, Eliana Herminia Pellizzari, Selva Cigorraga, María Noel Galardo, Silvina Beatriz Meroni, María Fernanda Riera
Metformin is one of the most widely used anti-hyperglycemic agents for treating patients with type 2 diabetes and it has started to be used in pediatric population at ages when Sertoli cells are still proliferating. It is well known that follicle-stimulating hormone (FSH) is the major Sertoli cell mitogen. The aim of the study is to investigate a possible effect of Metformin, which has been shown to have anti-proliferative properties, on FSH regulation of postnatal Sertoli cell proliferation and on the molecular mechanisms involved in this regulation...
May 22, 2018: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/29789440/objective-evaluation-of-ram-and-buck-sperm-motility-by-using-novel-sperm-tracker-software
#19
Francisco Buchelly Imbachí, Lucia Zalazar, Juan Ignacio Pastore, Micaela Belén Greco, Maria Iniesta Cuerda, Jose Julian Garde, A J Soler, Virginia Ballarin, Andreina Cesari
This work offers researchers the first version of an open-source sperm tracker software (Sperm Motility Tracker V1.0) containing a novel suit of algorithms to analyze sperm motility using ram and buck sperm as models. The computer-assisted semen analysis (CASA) is used in several publications with increasing trend worldwide in the last years, showing the importance of objective methodologies to evaluate semen quality. However, commercial systems are costly and versatility is constrained. In the proposed method, segmentation is applied and the tracking stage is performed by using individual Kalman filters and a simplified occlusion handling method...
May 22, 2018: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/29752297/lactational-programming-of-glucose-homeostasis-a-window-of-opportunity
#20
Lindsay Ellsworth, Emma Harman, Vasantha Padmanabhan, Brigid Gregg
The window of lactation is a critical period during which nutritional and environmental exposures impact lifelong metabolic disease risk. Significant organ and tissue development, organ expansion, and maturation of cellular functions occur during the lactation period, making this a vulnerable time during which transient insults can have lasting effects. This review will cover current literature on factors influencing lactational programming such as milk composition, maternal health status, and environmental endocrine disruptors...
May 11, 2018: Reproduction: the Official Journal of the Society for the Study of Fertility
journal
journal
35194
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"