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Reproduction: the Official Journal of the Society for the Study of Fertility

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https://www.readbyqxmd.com/read/28188160/endogenous-hydrogen-sulfide-contributes-to-uterine-quiescence-during-pregnancy
#1
Xingji You, Zixi Chen, Huina Zhao, Chen Xu, Weina Liu, Qianqian Sun, Ping He, Hang Gu, Xin Ni
Recent evidence suggests that uterine activation for labor is associated with inflammation within uterine tissues. Hydrogen sulfide (H2S) plays a critical role in inflammatory responses in various tissues. Our previous study has shown that human myometrium produces H2S via its generating enzymes cystathionine-γ-lyase (CSE) and cystathionine-β-synthetase (CBS) during pregnancy. We therefore explored whether H2S plays a role in the maintenance of uterine quiescence during pregnancy. Human myometrial biopsies were obtained from pregnant women at term...
February 10, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28174321/time-course-effect-of-lps-on-tlrs-expression-and-steroidogenesis-in-the-goose-ovary
#2
ShiJia Ying, JiaJia Guo, ZiChun Dai, HuanXi Zhu, JianNing Yu, WeiMing Ma, JiaYI Li, Muhammad Faheem Akhtar, ZhenDan Shi
The ovary of Chinese goose is easily infected by microorganisms because of mating behaviour on water, which causes decreased laying performance. This study investigated the time course effect of lipopolysaccharide (LPS) on the steroidogenesis and mRNA expression of Toll-like receptors (TLRs), a class of key pattern recognition receptor, in the breeding goose ovary. The laying geese were treated intravenously with LPS for 0, 6, 12, 24 and 36 h, and all birds were slaughtered approximately 8 h after oviposition...
February 7, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28174320/isolation-of-luteal-endothelial-cells-and-functional-interactions-with-t-lymphocytes
#3
Sadhat S Walusimbi, Lindy Wetzel, David Townson, Joy Pate
The objectives of this study were to optimize the isolation of luteal endothelial cells (LEC) and examine their functional interactions with autologous T lymphocytes. Analysis by flow cytometry showed that the purity of LEC isolated by filtration was nearly 90% as indicated by Bandeiraea simplicifolia (BS)-1 lectin binding. LEC expressed mRNA for progesterone receptor (PGR), prostaglandin receptors (PTGFR, PTGER2 and 4, and PTGIR), tumor necrosis factor receptors (TNFRSF1A&B) and interleukin (IL) 1B receptors (IL1R1&2)...
February 7, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28154111/molecular-evidence-that-follicle-development-is-accelerated-in-vitro-compared-to-in-vivo
#4
Véronique Cadoret, Cynthia Frapsauce, Peggy Jarrier, Virginie Maillard, Agnès Bonnet, Yann Locatelli, Dominique Royère, Danielle Monniaux, Fabrice Guérif, Philippe Monget
In this study we systematically compared the morphological, functional and molecular characteristics of granulosa cells and oocytes obtained by a three-dimensional in vitro model of ovine ovarian follicular growth with those of follicles recovered in vivo Preantral follicles of 200 µm diameter were recovered and cultured up to 950 µm over a 20-day period. Compared with in vivo follicles, the in vitro culture conditions maintained follicle survival, with no difference in the rate of atresia. However, the in vitro conditions induced a slight decrease in oocyte growth rate, delayed antrum formation and increased granulosa cell proliferation rate, accompanied by an increase and decrease in CCND2 and CDKN1A mRNA expression, respectively...
February 2, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28137896/vitamin-d-deficiency-and-impaired-placental-function-potential-regulation-by-glucocorticoids
#5
Nathanael Yates, Rachael Christina Crew, Caitlin Wyrwoll
Maternal vitamin D deficiency has been implicated in a range of pregnancy complications including preeclampsia, preterm birth and intrauterine growth restriction. Some of these adverse outcomes arise from alterations in placental function. Indeed, vitamin D appears critical for implantation, inflammation, immune function and angiogenesis of the placenta. Despite these associations, absence of placental vitamin D receptor in mice provokes little effect. Thus, interactions between maternal and fetal compartments are likely crucial for instigating adverse placental changes...
January 30, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28123059/overactivation-of-hedgehog-signaling-interferes-with-regression-of-the-m%C3%A3-llerian-duct
#6
Fernando F Migone, Pei-Hsuan Hung, Robert G Cowan, Vimal Selvaraj, Susan Suarez, Susan M Quirk
The influence of the hedgehog signaling pathway on reproduction was studied in transgenic mice in which a dominant active allele of the hedgehog signal transducer, smoothened (SMOM2), was conditionally expressed in the developing Müllerian duct and gonads through recombination mediated by anti-Müllerian hormone receptor 2-cre (Amhr2cre). Previous studies showed that development of the oviduct and uterus are abnormal in female Amhr2cre/+SmoM2 mice. In the current study, focusing on mutant males, litter size was reduced 53% in crosses with wild-type females...
January 25, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28115581/differential-expression-of-cart-in-ewes-with-differing-ovulation-rates
#7
Jennifer L Juengel, Michelle C French, Laurel D Quirke, Alexia Kauff, George W Smith, Peter D Johnstone
We hypothesised that cocaine- and amphetamine-regulated transcript (CARTPT) would be differentially expressed in ewes with differing ovulation rates. Expression of mRNA for CARTPT, as well as LHCGR, FSHR, CYP19A1 and CYP17A1 was determined in antral follicles >1 mm in diameter collected during the follicular phase in ewes heterozygous for the Booroola and Inverdale genes (I+B+; average ovulation rate 4) and ++ contemporaries (++; average ovulation rate 1.8). In ++ ewes (n=6), CARTPT was expressed in small follicles (1-<3 mm diameter), where 18...
January 23, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28115580/role-of-the-testis-interstitial-compartment-in-spermatogonial-stem-cell-function
#8
Sarah J Potter, Tony DeFalco
Male fertility is maintained through intricate cellular and molecular interactions that ensure spermatogonial stem cells (SSCs) proceed in a step-wise differentiation process through spermatogenesis and spermiogenesis to produce sperm. SSCs lie within the seminiferous tubule compartment, which provides a nurturing environment for the development of sperm. Cells outside of the tubules, such as interstitial and peritubular cells, also help direct SSC activity. This review focuses on interstitial (interstitial macrophages, Leydig cells, and vasculature) and peritubular (peritubular macrophages, peritubular myoid cells) cells and their role in regulating SSC self-renewal and differentiation in mammals...
January 23, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28115579/the-role-of-sex-steroids-in-white-adipose-tissue-adipocyte-function
#9
Annie E Newell-Fugate
With the increasing knowledge that gender impacts normal physiology, much biomedical research has begun to focus on the differential effects of sex on tissue function. Sexual dimorphism in mammals is due to the combined effects of both genetic and hormonal factors. Hormonal factors are mutable particularly in females where the estrous cycle dominates the hormonal milieu. Given the severity of the obesity epidemic and the fact that there are differences in obesity rates in men and women, the role of sex in white adipose tissue function is being recognized as increasingly important...
January 23, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28104825/effect-of-bovine-oviductal-extracellular-vesicles-on-embryo-development-and-quality-in-vitro
#10
Ricaurte Lopera-Vasquez, Meriem Hamdi, Veronica Maillo, Alfonso Gutierrez-Adan, Pablo Bermejo-Alvarez, Miguel Angel Ramirez, Maria Yañez-Mo, Dimitrios Rizos
The aim of this study was to evaluate the effect of extracellular vesicles (EV) from oviductal fluid (OF), either from the ampulla or isthmus, on the development and quality of in vitro cultured bovine embryos. Zygotes were cultured in synthetic oviduct fluid (SOF+3mg/ml BSA) without calf serum (C- group), in the presence of 3x105 EV/ml from ampullary or isthmic OF at either 1x104xg (10k) and 1x105xg (100k), and compared with SOF+5% FCS (C+ group). OF-EV size and concentration were assessed by electron microscopy and nanotracking analysis system...
January 19, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28104824/gnrh-mrna-expression-in-the-brain-of-cooperatively-breeding-female-damaraland-mole-rats
#11
Cornelia Voigt, Nc Bennett
The Damaraland mole-rat (Fukomys damarensis) is a eusocial, subterranean rodent, in which breeding is limited to a single reproductive pair within each colony. Non-reproductive females, while in the confines of the colony, exhibit socially-induced infertility. Anovulation is thought to be caused by a disruption in the normal gonadotropin-releasing hormone (GnRH) secretion from the hypothalamus. In order to assess whether social suppression is associated with altered GnRH mRNA expression in the brain we investigated the distribution and gene expression levels by means of in situ hybridization in female breeders and non-breeders from field captured colonies of the Damaraland mole-rat...
January 19, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28073824/a-drosophila-toolkit-for-defining-gene-function-in-spermatogenesis
#12
Nicole A Siddall, Gary R Hime
Expression profiling and genomic sequencing methods enable the accumulation of vast quantities of data that relate to expression of genes during the maturation of male germ cells from primordial germ cells to spermatozoa, and potential mutations that underlie male infertility. However, determination of gene function in specific aspects of spermatogenesis or linking abnormal gene function with infertility remain rate limiting, as even in an era of CRISPR analysis of gene function in mammalian models, this still requires considerable resources and time...
January 10, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28069904/detection-and-activity-of-11-beta-hydroxylase-cyp11b1-in-the-bovine-ovary
#13
Ayelen N Amweg, Fernanda Mariel Rodriguez, Emilia Huber, Belkis E Marelli, Natalia Gareis, Eduardo M Belotti, Florencia Rey, Natalia Raquel Salvetti, Hugo H Ortega
Glucocorticoids (GCs) such as cortisol and corticosterone are important steroid hormones with different functions in intermediate metabolism, development, cell differentiation, immune response and reproduction. In response to physiological and immunological stress, adrenocorticotropic hormone (ACTH) acts on the adrenal gland by stimulating synthesis and secretion of GCs. However, there is increasing evidence that GCs may also be synthesized by extra-adrenal tissues. Here, we examined the gene and protein expression of the enzyme 11β-hydroxylase P450c11 (CYP11B1), involved in the conversion of 11-deoxycortisol to cortisol, in the different components of the bovine ovary, and determined the functionality of CYP11B1 in vitro...
January 9, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28069903/improvement-of-in-vitro-produced-bovine-embryo-treated-with-coa-a-under-heat-stressed-condition
#14
Imran Khan, Kyeong-Lim Lee, Lianguang Xu, Ayman Mesalam, Mmr Chowdhury, Myeong-Don Joo, Ihsan-Ul- Haq, Bushra Mirza, Il-Keun Kong
Heat stress has large effects on reproduction including conception rate in cattle. In this study, we examined the effects of coagulansin-A (coa-A), a steroidal lactone, on acquired thermo tolerance during in vitro production of bovine embryos. Oocytes were incubated in in vitro maturation (IVM) media with or without coa-A at two different temperatures, 40.5 °C and 42 °C, for 20 h. The treatment of coa-A significantly improved blastocyst development only at 40.5 °C (P < 0.05). Interestingly, immunofluorescence analysis demonstrated that coa-A induced heat shock protein 70 (HSP70) and phosphatidylinositol-3-kinase (PI3K), but significantly attenuated nuclear factor kappa B (NF-kB) and cyclooxygenase-2 (COX2)...
January 9, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28069902/wnt-regulation-of-embryonic-development-likely-involves-pathways-independent-of-nuclear-ctnnb1
#15
Paula Tribulo, James I Moss, Manabu Ozawa, Zongliang Jiang, Cindy Tian, Peter J Hansen
The bovine was used to examine potential for WNT signaling to affect the preimplantation embryo. Expression of seven key genes involved in canonical WNT signaling declined to a nadir at the morula or blastocyst stage. Expression of 80 genes associated with WNT signaling in the morula and inner cell mass (ICM) and trophectoderm (TE) of the blastocyst was also evaluated. Many genes associated with WNT signaling were characterized by low transcript abundance. Seven genes were different between ICM and TE, and all of them were overexpressed in TE as compared to ICM, including WNT6, FZD1, FZD7, LRP6, PORCN, APC, and SFRP1...
January 9, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28069901/bmp15-regulates-the-inhibin-activin-system-independently-of-ovulation-rate-control-in-sheep
#16
Anthony Estienne, Belén Lahoz, Peggy Jarrier, Loys Bodin, José Folch, José Luis Alabart, Stephane Fabre, Danielle Monniaux
Polymorphisms in the gene encoding bone morphogenetic protein 15 (BMP15) have been associated with multiple ovulations in sheep. As BMP15 regulates inhibin expression in rodents, we assumed that the ovarian inhibin/activin system could mediate part of the effect of BMP15 mutations in the regulation of ovulation rate in sheep. To answer this question, we have studied the effects of two natural loss-of-function mutations of BMP15 on the expression of components of this system. The FecX(R) and the FecX(Gr) mutations, when present respectively in Rasa Aragonesa ewes at the heterozygous state and in Grivette ewes at the homozygous state, were associated with a two-fold increase in ovulation rate...
January 9, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28069900/impact-of-donor-and-recipient-adiposity-on-feto-placental-growth-in-adolescent-sheep
#17
Jacqueline M Wallace, John S Milne, Clare L Adam, Raymond P Aitken
The influence of maternal obesity during oocyte development and its putative interaction with nutrient reserves at conception on pregnancy outcome were examined in an adolescent sheep model. Donor ewes were nutritionally managed to achieve contrasting adiposity (control [CD]/obese [ObD]) for 6 weeks prior to superovulation, and inseminated by a non-obese sire. Morulae from 6 CD and 7 ObD were transferred in singleton into adolescent recipients of identical age but differing adiposity, classified as relatively fat or thin, respectively...
January 9, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/27998941/mir-361-3p-regulates-fsh-by-targeting-fshb-in-a-porcine-anterior-pituitary-cell-model
#18
Rui-Song Ye, Meng Li, Chao-Yun Li, Qi-En Qi, Ting Chen, Xiao Cheng, Songbo Wang, Gang Shu, Li-Na Wang, Xiao-Tong Zhu, Qingyan Jiang, Qian-Yun Xi, Yong-Liang Zhang
FSH plays an essential role in processes involved in human reproduction, including spermatogenesis and the ovarian cycle. While the transcriptional regulatory mechanisms underlying its synthesis and secretion have been extensively studied, little is known about its post-transcriptional regulation. A bioinformatics analysis from our group indicated that a microRNA (miRNA; miR-361-3p) could regulate FSH secretion by potentially targeting the FSHB subunit. Herein, we sought to confirm these findings by investigating the miR-361-3p-mediated regulation of FSH production in primary pig anterior pituitary cells...
December 20, 2016: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/27965401/bovine-pirna-like-rnas-are-associated-with-both-mobile-elements-and-mrnas
#19
Stewart J Russell, Mehool Patel, Graham Gilchrist, Leanne Stalker, Daniel Gillis, David Rosenkranz, Jonathan LaMarre
PIWI proteins and their associated piRNAs have been the focus of intensive research for the past decade, and their participation in the maintenance of genomic integrity during spermatogenesis has been well established. Recent studies have suggested important roles for the PIWI/piRNA system outside of gametogenesis, based on the presence of piRNAs and PIWI proteins in several somatic tissues, cancers, and the early embryo. Here we investigated the small-RNA complement present in bovine gonads, gametes, and embryos through next generation sequencing...
December 13, 2016: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/27965400/decreased-levels-of-srage-in-follicular-fluid-from-patients-with-pcos
#20
BiJun Wang, Jing Li, Qingling Yang, FuLi Zhang, MengMeng Hao, Yi-Hong Guo
This study aimed to explore the association between soluble receptor for advanced glycation endproducts (sRAGE) levels in follicular fluid and the number of oocytes retrieved and to evaluate the effect of sRAGE on vascular endothelial growth factor (VEGF) in granulosa cells in patients with polycystic ovarian syndrome (PCOS). Two sets of experiments were performed in this study. In part one, sRAGE and VEGF protein levels in follicular fluid samples from 39 patients with PCOS and 35 non-PCOS patients were measured by ELISA...
December 13, 2016: Reproduction: the Official Journal of the Society for the Study of Fertility
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