journal
https://read.qxmd.com/read/37839014/the-effect-of-caffeine-intake-and-passive-smoking-on-umbilical-cord-blood-unit-s-quality-parameters
#21
JOURNAL ARTICLE
Walid Almashaqbeh, Husam Ardah, Amal Alasmari, Anadel Hakeem, Shahad Alsaigh, Amairah Aloushan, Dunia Jawdat
Today cord blood (CB) is a valuable source of hematopoietic stem cells to treat many hematological disorders. One of the limitations of CB utilization is the reduced number of nucleated cells including stem cells. Therefore, CB banks around the world have developed strategies in an attempt to improve donor selection and the quality of the CB inventory. This study aimed to determine the impact of passive smoking and caffeine consumption on CB quality. CBs were obtained from mothers who gave birth at King Abdulaziz Medical City...
October 15, 2023: Cell and Tissue Banking
https://read.qxmd.com/read/37812368/an-overview-of-the-production-of-tissue-extracellular-matrix-and-decellularization-process
#22
REVIEW
Shima Dehghani, Zahra Aghaee, Safoura Soleymani, Maryam Tafazoli, Yasin Ghabool, Amin Tavassoli
Thousands of patients need an organ transplant yearly, while only a tiny percentage have this chance to receive a tissue/organ transplant. Nowadays, decellularized animal tissue is one of the most widely used methods to produce engineered scaffolds for transplantation. Decellularization is defined as physically or chemically removing cellular components from tissues while retaining structural and functional extracellular matrix (ECM) components and creating an ECM-derived scaffold. Then, decellularized scaffolds could be reseeded with different cells to fabricate an autologous graft...
October 9, 2023: Cell and Tissue Banking
https://read.qxmd.com/read/37804396/retraction-note-approaches-to-reducing-the-toxic-exposure-hazard-on-the-sheep-population
#23
Mynbay Umitzhanov, Asiya K Musaeva, Abdikalyk A Abishov, Toktar M Zhamansarin, Urzhan Zh Omarbekova, Sholpan Zh Turyspayeva, Sarsenbek T Siyabekov
No abstract text is available yet for this article.
October 7, 2023: Cell and Tissue Banking
https://read.qxmd.com/read/37792171/using-cryopreserved-allogeneic-pericardium-to-repair-congenital-heart-defects-in-children
#24
JOURNAL ARTICLE
Mariusz Birbach, Maciej Fedorowicz, Ewa M Gałkowska, Agnieszka Powirska, Michał Kozłowski, Krzysztof Mozol, Aleksandra Wasiak, Bohdan Maruszewski, Andrzej Kansy
Patches prepared from autologous, allogeneic, or xenogeneic tissues are widely used in the repair of congenital heart defects in children. Since 2002, cryopreserved allogeneic pericardial patches have been prepared in our institution as an alternative to commercially available patches. This study retrospectively reviewed donor and patient data concerning cryopreservation time and the clinical use of the pericardium in 382 children who were operated on at a single center between 2004 and 2021. There were 177 donors: 98 males and 79 females...
October 4, 2023: Cell and Tissue Banking
https://read.qxmd.com/read/37776436/effect-of-crocin-and-quercetin-supplementation-in-cryopreservation-medium-on-post-thaw-human-sperm-quality
#25
JOURNAL ARTICLE
Ensieh Salehi, Amir Shadboorestan, Afshin Mohammadi-Bardbori, Abedeh Mousavi, Elias Kargar-Abargouei, Parisa Sarkoohi, Mahmoud Omidi
Biochemical and physical changes during the cryopreservation process adversely affect sperm function required for fertilization. Recently, many studies have been conducted to find effective pre-freezing treatments to limit these damages. The present study aimed to investigate the effects of pre-freezing treatment with quercetin and crocin, individually or in combination, on sperm parameters after thawing procedure. For this, semen samples from 20 normozoospermic men were collected and then each sample was divided into five equal parts: 1...
September 30, 2023: Cell and Tissue Banking
https://read.qxmd.com/read/37728671/validation-of-orthopedic-allograft-preparation-process-a-new-application-of-media-fill-test
#26
JOURNAL ARTICLE
Andrea Ossato, Carlo Gasperoni, Michela Stella, Giulia Montagner, Diletta Trojan, Giuseppe Giovagnoni, Michele Giannini, Francesca Realdon, Valeria Mezzadrelli, Nicola Realdon, Teresa Zuppini, Roberto Tessari
Musculoskeletal allografts represent an important practice in orthopedic surgeries and the demand for them has been growing. For this reason, in order to reduce clinical risk and to more efficiently manage the increase of allograft usage and also to optimize timing of the surgeries, the thawing and washing processes with aseptic technique were centralized in the department of Hospital Pharmacy. This study describe the design and execution of an adapted Media Fill Test (MFT) to demonstrate aseptic thawing and washing of allografts...
September 20, 2023: Cell and Tissue Banking
https://read.qxmd.com/read/37676366/hyaluronic-acid-silicon-nanoparticle-scaffold-induces-proliferation-and-differentiation-of-mouse-spermatogonial-stem-cells-transplanted-to-epididymal-adipose-tissue
#27
JOURNAL ARTICLE
Saber Saharkhiz, Zohreh Abdolmaleki, Mohammad Amin Eslampour
Spermatogonia stem cells (SSCs) are a unique cell population maintaining male spermatogenesis during life, through their potential for proliferation and differentiation. The application of silicon nanoparticles (SNs) and hyaluronic acid (HA) to induce the differentiation of SSCs seems promising. Herein, we investigate the effect of SN and HA scaffolds on the progression of SSCs spermatogenesis in mice. Initially SSCs were isolated from healthy immature mice and cultured on prepared scaffolds (HA, SN, and HA/SN) in a 3D culture system...
September 7, 2023: Cell and Tissue Banking
https://read.qxmd.com/read/37660321/characterization-and-biocompatibility-evaluation-of-acellular-rat-skin-scaffolds-for-skin-tissue-engineering-applications
#28
JOURNAL ARTICLE
Leila Taghizadeh Momen, Arash Abdolmaleki, Asadollah Asadi, Saber Zahri
Utilization of acellular scaffolds, extracellular matrix (ECM) without cell content, is growing in tissue engineering, due to their high biocompatibility, bioactivity ad mechanical support. Hence, the purpose of this research was to study the characteristics and biocompatibility of decellularized rat skin scaffolds using the osmotic shock method. First, the skin of male Wistar rats was harvested and cut into 1 × 1 cm2 pieces. Then, some of the harvested parts were subjected to the decellularization process by applying osmotic shock...
September 3, 2023: Cell and Tissue Banking
https://read.qxmd.com/read/37624485/preanalytical-variables-influencing-the-interpretation-and-reporting-of-biological-tests-on-blood-samples-of-living-and-deceased-donors-for-human-body-materials
#29
JOURNAL ARTICLE
Elizaveta Padalko, Luc Colenbie, Alain Delforge, Nadine Ectors, Johan Guns, Romain Imbert, Hilde Jansens, Jean-Paul Pirnay, Marie-Pierre Rodenbach, Ivan Van Riet, Anne Vansteenbrugge, Gilbert Verbeken, Muriel Baltes, Hilde Beele
With the present paper, the Working Group on Cells, Tissues and Organs and other experts of the Superior Health Council of Belgium aimed to provide stakeholders in material of human origin with advice on critical aspects of serological and nucleic acid test (NAT) testing, to improve virological safety of cell- and tissue and organ donation. The current paper focusses on a number of preanalytical variables which can be critical for any medical biology examination: (1) sampling related variables (type of samples, collection of the samples, volume of the sample, choice of specific tubes, identification of tubes), (2) variables related to transport, storage and processing of blood samples (transport, centrifugation and haemolysis, storage before and after centrifugation, use of serum versus plasma), (3) variables related to dilution (haemodilution, pooling of samples), and (4) test dependent variables (available tests and validation)...
August 25, 2023: Cell and Tissue Banking
https://read.qxmd.com/read/37615839/retraction-note-investigation-of-the-mechanism-of-gamma-irradiation-effect-on-bovine-bone
#30
Noor Rahman, Rafiullah Khan, Tanvir Hussain, Nabi Ahmed
No abstract text is available yet for this article.
August 24, 2023: Cell and Tissue Banking
https://read.qxmd.com/read/37578672/effect-of-gamma-and-ultraviolet-c-sterilization-on-bmp-7-level-of-indigenously-prepared-demineralized-freeze-dried-bone-allograft
#31
JOURNAL ARTICLE
Zainab Kamal, Arundeep Kaur Lamba, Farrukh Faraz, Shruti Tandon, Archita Datta, Nasreen Ansari, Zaid Kamal Madni, Jaya Pandey
The presence of bone morphogenetic proteins in demineralized freeze-dried bone allograft (DFDBA) are responsible for developing hard tissues in intraosseous defects. The most common mode of sterilization of bone allografts, i.e., Gamma rays, have dramatic effects on the structural and biological properties of DFDBA, leading to loss of BMPs. Ultraviolet-C radiation is a newer approach to sterilize biodegradable scaffolds, which is simple to use and ensures efficient sterilization. However, UV-C radiation has not yet been effectively studied to sterilize bone allografts...
August 14, 2023: Cell and Tissue Banking
https://read.qxmd.com/read/37550401/effect-of-storage-media-on-chondrocyte-viability-during-cold-storage-of-osteochondral-dowels
#32
JOURNAL ARTICLE
Noor Abdelwahab, Mohammadhamed Shahsavari, Kezhou Wu, Leila Laouar, Tamara D Skene-Arnold, Janet A W Elliott, Nadr M Jomha
Osteochondral allograft transplantation is a successfully proven method to repair articular cartilage defects and prevent the degenerative effects of osteoarthritis. The number of osteochondral transplantations that can be performed each year is limited by availability of donor cartilage tissue and storage time constraints. Osteochondral transplantation success has been linked to high chondrocyte viability of the donor cartilage tissue at the time of implantation. Determining optimal storage conditions for donor cartilage is essential for tissue banks to safely provide quality cartilage tissue...
August 8, 2023: Cell and Tissue Banking
https://read.qxmd.com/read/37542003/evaluation-of-bone-marrow-derived-mesenchymal-stem-cells-with-eggshell-membrane-for-full-thickness-wound-healing-in-a-rabbit-model
#33
JOURNAL ARTICLE
S Amitha Banu, Abhijit M Pawde, Khan Sharun, E Kalaiselvan, Shivaraju Shivaramu, Karikalan Mathesh, Vikas Chandra, Rohit Kumar, Swapan Kumar Maiti, Med Ram Verma, Karam Pal Singh, Amarpal
Biomaterials capable of managing wounds should have essential features like providing a natural microenvironment for wound healing and as support material for stimulating tissue growth. Eggshell membrane (ESM) is a highly produced global waste due to increased egg consumption. The unique and fascinating properties of ESM allow their potential application in tissue regeneration. The wound healing capacity of bone marrow-derived mesenchymal stem cells (BM-MSCs), ESM, and their combination in rabbits with full-thickness skin defect (2 × 2 cm2 ) was evaluated...
August 4, 2023: Cell and Tissue Banking
https://read.qxmd.com/read/37501011/platelet-rich-plasma-alleviates-knee-arthritis-in-rats-by-inhibiting-p65
#34
JOURNAL ARTICLE
Feng Zhuo, Xiaojing Jia, Zongru Wang, Yeyong Zhang, Xinfeng Yan
Knee osteoarthritis (KOA) is a chronic joint disease characterized by the degeneration of articular cartilage. In this study, we explored the potential therapeutic effects of platelet-rich plasma (PRP) and identified molecular targets for treating KOA. A rat model of KOA was established via the Hulth method and primary knee joint chondrocytes were isolated to evaluate the effects of PRP and shRNA targeting p65 (sh-p65). ELISA was used to detect inflammatory factors, including IL-6, IL-1β, and TNF-α...
July 27, 2023: Cell and Tissue Banking
https://read.qxmd.com/read/37468823/activated-platelet-rich-plasma-accelerate-endometrial-regeneration-and-improve-pregnancy-outcomes-in-murine-model-of-disturbed-endometrium
#35
JOURNAL ARTICLE
Jeevitaa Kshersagar, Akshay A Kawale, Kishor Tardalkar, Mrunal N Damle, Leena R Chaudhari, Rakesh Sharma, Meghnad G Joshi
Platelet Rich Plasma (PRP) contains high concentrations of growth factors, therefore, PRP activation results in their release, stimulating the process of healing and regeneration. The study was conducted to check whether activated platelet-rich plasma (aPRP) treatment can improve regeneration of the endometrium in an experimental model of ethanol-induced disturbed endometrium. Seventy-two female Wistar rats were randomly assigned into the control group, disturbed endometrium (DE) group and aPRP treated group...
July 19, 2023: Cell and Tissue Banking
https://read.qxmd.com/read/37368142/culture-of-cerebrospinal-fluid-contacting-neurons-from-neonatal-mouse-spinal-cord
#36
JOURNAL ARTICLE
Liang Cao, Hui-Qian Zhang, Yu-Qi He, Ping-Jiang An, Lei-Luo Yang, Wei Tan, Gang Liu, Chun-Qing Wang, Xiao-Wei Dou, Qing Li
Cerebrospinal fluid-contacting neurons (CSF-cNs) act crucial role in chemosensory and mechanosensory function in spinal cord. Recently, CSF-cNs were found to be an immature neuron and may be involved in spinal cord injury recovery. But how to culture it and explore its function in vitro are not reported in previous research. Here, we first reported culture and identification of CSF-cNs in vitro. We first established a protocol for in vitro culture of CSF-cNs from the cervical spinal cord of mice within 24 h after birth...
June 27, 2023: Cell and Tissue Banking
https://read.qxmd.com/read/37365484/the-role-of-oxygen-tension-in-cell-fate-and-regenerative-medicine-implications-of-hypoxia-hyperoxia-and-free-radicals
#37
REVIEW
Mehdi Rasouli, Roya Fattahi, Ghader Nuoroozi, Zeinab Zarei-Behjani, Maliheh Yaghoobi, Zeinab Hajmohammadi, Simzar Hosseinzadeh
Oxygen pressure plays an integral role in regulating various aspects of cellular biology. Cell metabolism, proliferation, morphology, senescence, metastasis, and angiogenesis are some instances that are affected by different tensions of oxygen. Hyperoxia or high oxygen concentration, enforces the production of reactive oxygen species (ROS) that disturbs physiological homeostasis, and consequently, in the absence of antioxidants, cells and tissues are directed to an undesired fate. On the other side, hypoxia or low oxygen concentration, impacts cell metabolism and fate strongly through inducing changes in the expression level of specific genes...
June 26, 2023: Cell and Tissue Banking
https://read.qxmd.com/read/37355504/the-trend-of-allogeneic-tendon-decellularization-literature-review
#38
REVIEW
Yangyang Jin, Qi Sun, Rongxing Ma, Ruifeng Li, Ruiqi Qiao, Jikai Li, Limin Wang, Yongcheng Hu
Tendon injuries repair is a significant burden for orthopaedic surgeons. Finding a proper graft material to repair tendon is one of the main challenges in orthopaedics, for which the requirement of substitute for tendon repair would be different for each clinical application. Among biological scaffolds, the use of decellularized tendon increasingly represents an interesting approach to treat tendon injuries and several articles have investigated the approaches of tendon decellularization. To understand the outcomes of the the approaches of tendon decellularization on effect of tendon transplantation, a literature review was performed...
June 24, 2023: Cell and Tissue Banking
https://read.qxmd.com/read/36074213/improvement-of-human-sperm-properties-with-platelet-rich-plasma-as-a-cryoprotectant-supplement
#39
JOURNAL ARTICLE
Maryam Sadat Nabavinia, Arash Yari, Saeed Ghasemi-Esmailabad, Aida Gholoobi, Lida Gholizadeh, Ali Nabi, Marzieh Lotfi, Mohammad Ali Khalili
PURPOSE: Platelet-rich plasma (PRP) is a remarkable substance, which involves the growth and proliferation of all cell types. As a source of growth factors, we evaluated whether sperm cryopreservation supplemented with PRP improves the rates of sperm motility, viability, and DNA integrity after vitrification compared with conventional cryo-medium. MATERIALS AND METHODS: 20 normal semen specimens were collected from healthy men. After swim-up preparation, each sample was divided into four aliquots...
June 2023: Cell and Tissue Banking
https://read.qxmd.com/read/37227562/inter-placental-variability-is-not-a-major-factor-affecting-the-healing-efficiency-of-amniotic-membrane-when-used-for-treating-chronic-non-healing-wounds
#40
JOURNAL ARTICLE
Vojtech Horvath, Alzbeta Svobodova, Joao Victor Cabral, Radovan Fiala, Jan Burkert, Petr Stadler, Jaroslav Lindner, Jan Bednar, Martina Zemlickova, Katerina Jirsova
This study aimed to evaluate the efficacy of cryopreserved amniotic membrane (AM) grafts in chronic wound healing, including the mean percentage of wound closure per one AM application, and to determine whether the healing efficiency differs between AM grafts obtained from different placentas. A retrospective study analyzing inter-placental differences in healing capacity and mean wound closure after the application of 96 AM grafts prepared from nine placentas. Only the placentas from which the AM grafts were applied to patients suffering from long-lasting non-healing wounds successfully healed by AM treatment were included...
May 25, 2023: Cell and Tissue Banking
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