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Cell and Tissue Banking

Letícia Kraft, Victoria Stadler Tasca Ribeiro, Luciana Cristina Wollmann, Paula Hansen Suss, Felipe Francisco Tuon
All cardiac allograft tissues are under potential contamination, requiring a validated terminal sterilization process or a minimal bioburden. The bioburden calculation is important to determine the bacterial burden and further decontamination and disinfection strategies for the valve processing. The aim of this study was to determine the bioburden from transport solution (TS) of heart valves obtained from non-heart-beating and heart-beating donors in different culture methods. The bioburden from TS was determined in 20 hearts donated for valve allograft tissue using membrane filter (MF) and direct inoculation...
March 19, 2018: Cell and Tissue Banking
Noëmi M C De Roo, Laurent A M Thierens, Liesbeth Temmerman, Guy A M De Pauw
Prior to cryopreservation, a tooth is transported from a contaminated oral environment to the tooth bank. Our objective was to identify all studies reporting or investigating a transport protocol prior to the cryopreservation of teeth, in terms of decontamination of the subjects. The systematic literature search (1970-2017) was based on MEDLINE via PubMed, Web of Science and the Cochrane Library. The reference lists of the included studies and the Science Citation Index were used for hand searching (snowballing)...
March 7, 2018: Cell and Tissue Banking
Jeevitaa Kshersagar, Ravi Kshirsagar, Shashikant Desai, Raghvendra Bohara, Meghnad Joshi
Direct application of amnion has greater risk of immunological rejection and infection. Decellularization is an effective method to lower the risk of immune complications and infections. The bioreactor assembly with multiple cassettes was designed for decellurization of multiple amnions with different cell types simultaneously in single run. A detergent-based protocol was modified to remove all cellular components from amnion and diminish the DNA content to render it non-immunogenic. Amnion (n = 10) were treated with 2% sodium dodecyl sulphate (SDS), 5% dimethyl sulfoxide (DMSO) and 2% sodium deoxycholeate (SD)...
March 5, 2018: Cell and Tissue Banking
Miroslav Špaček, Pavel Měřička, Libor Janoušek, Petr Štádler, Miloš Adamec, Robert Vlachovský, Igor Guňka, Pavel Navrátil, Filip Thieme, Petr Mitáš, Rudolf Špunda, Jaroslav Špatenka, Robert Staffa, Petr Němec, Jaroslav Lindner
The transplantation of fresh or cryopreserved vascular allografts in patients with a prosthetic graft infection or critical limb ischemia is necessary for their limb salvage and, in many cases, represents a lifesaving procedure. While transplantation of fresh allografts has a long history in the Czech Republic, the standard use of cryopreserved vascular allografts was introduced into the clinical practice in 2011 as a result of the implementation of EU Directive 2004/23/EC into national legislation (Human Cell and Tissue Act No...
March 3, 2018: Cell and Tissue Banking
Harish C Chandramoorthy, Abdulmajeed Mohammed Bajunaid, Hussian Nasser Kariri, Ahmed Al-Hakami, Abdullah Abu Sham, Misfer Bin Safer Al-Shahrani, Suliman M Al-Humayed, Prasanna Rajagopalan
We explored the possibility of the cryo-storage of cord blood hematopoietic stem cells (CBHPSC) with respect to the quantity, quality and biologic efficacy of high altitude (HA) region Abha against sea level (SL) region. The results of the post-processed total nucleated cell count was 8.03 ± 0.31 × 107 and 8.44 ± 0.23 × 107 cells in the HA and SL regions respectively. The mean post processing viability of the nucleated cells was about 87.03 ± 1.39 (HA) and 88.33 ± 1.55% (SL) while post thaw cells were 85...
February 19, 2018: Cell and Tissue Banking
Jianhong Qi, Famin Cao, Yunning Han, Di Xie, Hongqiang Song, Bin Chen, Lu Zhou
The purpose of this study is to evaluate the reliability of cartilage digestion and fluorescein diacetate-ethidium bromide (FDA-EB) fluorescence staining for the detection of chondrocyte viability in osteochondral grafts. Sixteen fresh osteochondral grafts were harvested from pig knee condyles, and the articular cartilage tissue was preserved. Each cartilage graft was cut into two 70-µm thick pieces and randomly allocated to Group A or Group B. The cell viability of Group A was detected using FDA-EB fluorescence staining of the digested cartilage, and the viability of Group B was detected with FDA-EB fluorescence staining of cartilage sections...
February 16, 2018: Cell and Tissue Banking
Zohreh Ghalavand, Alireza Heidary Rouchi, Hassan Bahraminasab, Elham Ravanasa, Elnaz Sadat Mirsamadi, Narges Nodeh Farahani, Bahram Nikmanesh
Microbiological screening of tissue allografts is crucial to prevent the transmission of bacterial and fungal infections to transplant recipients. Klebsiella was the most prevalent and resistant contaminating microorganism observed in our setting in the Iranian Tissue Bank. This study was conducted to determine the presence of extended-spectrum β-lactamase (ESBL) genes, antimicrobial resistance patterns of Klebsiella pneumoniae isolates, and their clonal relationships in allograft materials. K. pneumoniae contaminating bone and other tissue allografts recovered from deceased donors were identified and ESBL isolates were detected using a phenotypic confirmatory method...
February 3, 2018: Cell and Tissue Banking
Artur Kamiński, Hanna Rozenek, Jolanta Banasiewicz, Stanisław Wójtowicz, Artur Błoński, Krzysztof Owczarek
Job Demands-Resources model proposes that the development of burnout follows excessive job demands and lack of job resources. Job demands are predictive of feeling of exhaustion, and lack of job resources-disengagement from work. This pilot study investigated professional burnout and its correlates in employees of Polish cell and tissue banks, many of whom were involved in procurement and processing of tissues from deceased donors, as it was hypothesized that job burnout in this population might influence the effectiveness of cell and tissue transplantation network in our country...
February 3, 2018: Cell and Tissue Banking
Ana Paula Negreiros Nunes Alves, Edmar Maciel Lima Júnior, Nelson Sarto Piccolo, Marcelo José Borges de Miranda, Maria Elisa Quezado Lima Verde, Antônio Ernando Carlos Ferreira Júnior, Paulo Goberlânio de Barros Silva, Victor Pinheiro Feitosa, Tereza Jesus Pinheiro Gomes de Bandeira, Monica Beatriz Mathor, Manoel Odorico de Moraes
Tissue bioengineering development is a global concern and different materials are studied and created to be safe, effective and with low cost. Nile Tilapia skin had shown its biological potential as covers for the burn wound. This study evaluates the tilapia skin histological, collagen properties and tensiometric resistance, after treatment by different sterilization methods. Tilapia skin samples were submitted to two sterilization processes: (1) chemical, which consisted in two 2% chlorhexidin baths, followed by sequential baths in increasing glycerol concentrations; and (2) radiation, when glycerolized skin samples were submitted to gamma radiation at 25, 30 and 50 kGy...
January 29, 2018: Cell and Tissue Banking
Peter Myint
Malaria is one of the exclusion criteria used in selecting tissue donors and the absence of this information can lead to rejection of tissues for transplant. The studies on the malaria parasite have been confined to low dose attenuation of parasites in blood for transfusion purposes. There is no published information relating to the inactivation of malaria parasites with irradiation for the sterilisation of tissues. A dose-surviving parasite population following radiation was replotted using D0 value from a published paper whereby D10 value of 41 Gy was obtained...
January 16, 2018: Cell and Tissue Banking
P Hogg, P Rooney, R Lomas, J N Kearney
This study was carried out to investigate leakage/transport across the bag material of six outer cryopreservation bags in common use within NHS Blood and Transplant. In order to do this two different leak testing procedures; coloured dye and hydrogen tracer gas, were used. The data obtained show that a coloured dye cannot permeate through the materials both at room temperature and following storage at liquid nitrogen temperature (- 196 °C). In addition, when filled with the smallest elemental molecule, hydrogen, in the form of a tracer gas, all of the bags only allowed trace amounts of hydrogen to escape, either through the seal or the bag material...
January 12, 2018: Cell and Tissue Banking
Sonia Iranpour, Nasser Mahdavi-Shahri, Raheleh Miri, Halimeh Hasanzadeh, Hamid Reza Bidkhori, Hojjat Naderi-Meshkin, Ehsan Zahabi, Maryam M Matin
Human amniotic membrane (HAM) has been widely used as a natural scaffold in tissue engineering due to many of its unique biological properties such as providing growth factors, cytokines and tissue inhibitors of metalloproteinases. This study aimed at finding the most suitable and supportive layer of HAM as a delivery system for autologous or allogeneic cell transplantation. Three different layers of HAM were examined including basement membrane, epithelial and stromal layers. In order to prepare the basement membrane, de-epithelialization was performed using 0...
January 8, 2018: Cell and Tissue Banking
Surena Vahabi, Zahra Yadegari, Hossein Mohammad-Rahimi
In the original publication of this article, the affiliation of the corresponding author has been published incorrectly. Now the correct affiliation has been provided in this erratum.
December 21, 2017: Cell and Tissue Banking
Michiel A J van de Sande, Judith V M G Bovée, Mark van Domselaar, Marja J van Wijk, Ingrid Sanders, Ed Kuijper
The current standard for sterilization of potentially infected bone graft by gamma irradiation and thermal or chemical inactivation potentially deteriorates the biomechanical properties of the graft. We performed an in vitro experiment to evaluate the use of high hydrostatic pressure (HHP); which is widely used as a disinfection process in the food processing industry, to sterilize bone grafts. Four femoral heads were divided into five parts each, of which 16 were contaminated (in duplicate) with 105-107 CFU/ml of Staphylococcus epidermidis, Bacillus cereus, or Pseudomonas aeruginosa or Candida albicans, respectively...
December 20, 2017: Cell and Tissue Banking
Hojjat Gholipour, Abdolhamid Meimandi-Parizi, Ahmad Oryan, Amin Bigham Sadegh
Fibrin-platelet glue (FPG) is a blood derivative, in which platelets and fibrinogen are concentrated in a small plasma volume, by differential centrifugation and precipitation. It can form a three-dimensional and biocompatible fibrin scaffold with a myriad of growth factors and proteins that are released progressively to the local environment and contribute to the accelerated postoperative bone healing. Gelatin (Gel) is a derivative of collagen and can promote cell adhesion and proliferation due to its unique sequence of amino acids, so it is suitable for bone tissue applications...
December 20, 2017: Cell and Tissue Banking
Martin J Dutch, Anthony F Denahy
Eye and Tissue donation has the capacity to transform lives, yet the vast majority of potential in-hospital donors are not recognised. Studies which describe the relative importance of specific units or wards in determining the size of the donor pool are limited. The aim of this study was to map the distribution of potential Eye and Tissue donors within the study hospital. A 12-month retrospective analysis of all patient deaths at the study hospital was undertaken. The ability to donate corneal, heart valve, bone and skin tissue was investigated...
December 11, 2017: Cell and Tissue Banking
Yongquan Gu, Fei Wang, Rong Wang, Jianxin Li, Cong Wang, Liqiang Li, Zeqin Xu, Jian Zhang
Decellularized arteries have been considered as promising scaffolds for small-diameter vascular substitutes. However, weakened mechanical properties, immunological rejection and rapid degradation after transplantation still exist after decellularization. Previous studies indicated that genipin cross-linking can solve these problems. Therefore, genipin was selected as the cross-linking agent for the pre-treatment of decellularized arteries in our study. Histological analysis, scanning electron microscopy, mechanical properties analysis and subcutaneous embedding experiment were adopted to investigate the efficiency of decellularization and the effect of genipin cross-linking on improving mechanical, structural and biological properties of decellularized arteries...
December 8, 2017: Cell and Tissue Banking
Shabnam Sabetkish, Nastaran Sabetkish, Mahba Ataei Talebi, Shahnaz Halimi, Abdol-Mohammad Kajbafzadeh
We compared the therapeutic effects of autologous and nonautologous adipose-derived mesenchymal stem cell (ADMSC), in ameliorating the renal function in a rabbit model of acute pyelonephritis. The difference of perirenal and neck subcutaneous ADMSCs were also evaluated. Twenty female rabbits were apportioned to 5 groups. In group I (n = 4), the rabbits were injected direct inoculation of Escherichia coli (E. coli) into the right kidney. In group II (n = 4), autologous ADMSCs obtained from nape adipose tissue were injected into the subcapsular space 1 week after E...
December 7, 2017: Cell and Tissue Banking
A Paolin, C Romualdi, L Romagnoli, D Trojan
The microbiological contamination of retrieved tissues has become a very important topic and it is a critical aspect in the safety of allografts, especially from multi-tissue donors whose tissues are frequently contaminated as a consequence of retrieval. We analysed a total of 10,107 tissues, 8178 musculoskeletal and 1929 cardiovascular tissues, retrieved from 978 multi-tissue donors. Of these, 159 heart-beating donors (HBD) were also organ donors, while the remaining 819 non-heart-beating donors (NHBD) were tissue donors only...
December 2017: Cell and Tissue Banking
Andreas Nicodemou, Lubos Danisovic
Mesenchymal stem (stromal) cells (MSCs) possess unique biological characteristics such as plasticity, long term self-renewal, secretion of various bioactive molecules and ability of active migration to the diseased tissues that make them unique tool for regenerative medicine, nowadays. Until now MSCs were successfully derived from many tissue sources including bone marrow, umbilical cord, adipose tissue, dental pulp etc. The crucial step prior to their in vitro expansion, banking or potential clinical application is their separation...
December 2017: Cell and Tissue Banking
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