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Cell and Tissue Banking

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https://www.readbyqxmd.com/read/29079991/establishment-of-a-porcine-corneal-endothelial-organ-culture-model-for-research-purposes
#1
Berenike C Kunzmann, Olaf J C Hellwinkel, Christian Klameth, Daniel Wenzel, Karl U Bartz-Schmidt, Martin S Spitzer, Maximilian Schultheiss
Human corneas usually are not available for research, as they are used for transplantation only. At the same time, scientific studies on cultured human endothelial cells can produce misleading results due to inevitable dedifferentiation. Therefore, an organ-culture model of porcine corneas-displaying endothelial cell death rates comparable to those of cultured human corneas-would be very desirable. Fresh pig eyes were prepared under sterile conditions to obtain corneoscleral buttons, corneal buttons and so called "split corneal buttons" (new preparation method) and cultivated for 15 days...
October 27, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29075945/use-of-freeze-dried-bone-allografts-in-osteoporotic-patients-undergoing-median-sternotomy
#2
Mei Liang, Jianzhou Liu, Qi Miao, Guotao Ma, Xingrong Liu, Xiaofeng Li, Chaoji Zhang
Osteoporosis is a major risk factor for deep sternal wound infection, which is a rare but serious complication after median sternotomy. We investigated the incidence of deep sternal wound infection and the protective effect of bone allografts in osteoporotic patients after sternal approximation. Data were collected retrospectively from consecutive osteoporotic patients who underwent cardiac surgery via median sternotomy. Sternal approximation in the historical control group was performed with conventional steel wire sutures...
October 26, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29071453/donor-cornea-tissue-in-cases-of-drowning-or-water-submersion-eye-banks-practice-patterns-and-tissue-outcomes
#3
Nithya P Vijayakumar, Purak Parikh, Shahzad I Mian, Brad Tennant, Gregory H Grossman, Bob Albrecht, Leslie M Niziol, Maria A Woodward
Surgical use of donor corneal tissue from victims of water submersion (drowning or submersion secondary to death) remains controversial due to limited evidence about the quality of these tissues. To assess the safety of donor corneal tissue from victims of water submersion, an investigation of eye banks' practice patterns and tissue outcomes was conducted. All 79 Eye Bank Association of America accredited eye banks were contacted for a phone interview of practices regarding tissue from victims of water submersion...
October 25, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29043524/improving-the-success-rate-of-human-corneal-endothelial-cell-cultures-from-single-donor-corneas-with-stabilization-medium
#4
D Spinozzi, A Miron, M Bruinsma, J T Lie, I Dapena, S Oellerich, G R J Melles
Main objective of this study was to improve the success rate of human corneal endothelial cell (hCEC) cultures from single donor corneas. We could show that the use of stabilization medium prior to cell isolation may have a positive effect on the success rate of hCEC cultures from single research-grade donor corneas by allowing growth of otherwise possibly not successful cultures and by improving their proliferative rate. hCEC were obtained from corneo-scleral rims of 7 discarded human research-grade cornea pairs...
October 17, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29043523/analysis-of-potential-factors-affecting-allografts-contamination-at-retrieval
#5
A Paolin, C Romualdi, L Romagnoli, D Trojan
The microbiological contamination of retrieved tissues has become a very important topic and it is a critical aspect in the safety of allografts, especially from multi-tissue donors whose tissues are frequently contaminated as a consequence of retrieval. We analysed a total of 10,107 tissues, 8178 musculoskeletal and 1929 cardiovascular tissues, retrieved from 978 multi-tissue donors. Of these, 159 heart-beating donors (HBD) were also organ donors, while the remaining 819 non-heart-beating donors (NHBD) were tissue donors only...
October 17, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29043522/cryovial-monolayer-vitrification-for-ovarian-tissue-cryopreservation
#6
Hongxia Li, Haixia Cao, Xingping Guo, Huaixiu Wang
The cryopreservation of ovarian tissue has been proved to be effective for fertility preservation. To find a better cryopreservation method, we tested the efficacy of cryovial monolayer vitrification method in comparison with that of needle immersed vitrification (NIV). Ovaries from 10 female kunming mice aged 6-8 weeks were cut into pieces and allocated into group A (cryovial monolayer vitrification method), group B (NIV method) and group C (fresh control). In group A, pieces of ovarian tissue were layered around the inner wall of cryovial as a monolayer; and in group B, pieces of ovarian tissue were pierced with a needle...
October 17, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29039070/the-evaluation-of-xenotransplantation-of-feline-ovarian-tissue-vitrified-by-needle-immersed-vitrification-technique-into-male-immunodeficient-mice
#7
Mürşide Ayşe Demirel, Duygu Baki Acar, Burcu Ekim, Ferda Topal Çelikkan, Kübra Karakaş Alkan, Seçkin Salar, Esra Atabenli Erdemli, Sinan Özkavukçu, Seda Sağlam Yar, Halit Kanca, Ayhan Baştan
In this study, the efficiency of the "Needle Immersed Vitrification" technique was tested on cryopreserved feline ovarian tissue. For vitrification, ovarian fragments (0.5-1.5 mm(2)) from each ovary were collected; the grafts were exposed to 7.5-15% ethylene glycol and 7.5-15% dimethyl sulfoxide at room temperature and stored in liquid nitrogen at least 1 week. Morphologic examinations, expression of genes such as B cell lymphoma 2, B-cell lymphoma-2-associated X protein, Bone morphogenetic protein 15, zone of polarizing activity, zona pellucida C protein and DNA (cytosine-5)-methyltransferase 1, ultrastructural analysis and viability tests were carried out from collected grafts...
October 16, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29032461/the-effect-of-ribose-pre-treatment-of-cortical-bone-on-%C3%AE-irradiation-sterilization-effectiveness
#8
Tarik Attia, Jindra Tupy, Dalal Asker, Benjamin Hatton, Marc Grynpas, Thomas Willett
Reconstruction of large skeletal defects is a significant and challenging issue. Tissue banks often use γ-irradiation (15-35 kGy) to sterilize bone allografts, which, however, drastically impairs the post-yield mechanical properties. In previous studies, we reported the development of a method that protects human bone collagen connectivity through ribose crosslinking while still undergoing γ-irradiation. Given these promising results, the next step was to determine if the presence of ribose within the bone tissue would interfere with the effectiveness of the γ-irradiation sterilization against bacteria...
October 14, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29027064/light-scattering-measurements-in-electron-beam-sterilized-corneas-stored-in-recombinant-human-serum-albumin
#9
Khoa D Tran, Yan Li, Jeffrey D Holiman, Maolong Tang, David Huang, Michael D Straiko, Christopher G Stoeger
Irradiated corneal tissues have been used for a variety of ophthalmic procedures including glaucoma drainage device covers and lamellar grafts. The maintenance of corneal clarity is important, as light obstructions resulting from processing or long-term storage of irradiated corneas may negatively affect vision and postoperative cosmesis. It has been reported that corneal tissues can be preserved in human serum albumin (HSA), however, the clarity of corneas after long-term storage in HSA has not been well described...
October 12, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29022196/advances-of-radiation-sterilisation-in-tissue-banking
#10
Norimah Yusof
Under the auspices of the IAEA tissue banking programme on "Radiation Sterilisation of Tissue Graft" conducted from 1985 to 2004, many scientists and surgeons were involved in various regional research and development (R&D) projects mainly in dealing with radiation dose selection, radiation effects on human tissues and quality system in radiation sterilisation. New findings on radiation effects, tissue processing and preservation were shared during the regional and interregional meetings and workshops. Many tissue banks started to use radiation (25 kGy) to sterilize tissue grafts for tissue safety and efficacy and still continue to use it...
October 11, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29022123/assessment-of-a-closed-wash-system-developed-for-processing-living-donor-femoral-heads
#11
M J Eagle, J Man, P Rooney, T A McQuillan, G Galea, J N Kearney
NHS Blood and Transplant Tissue and Eye Services (TES) and Scottish National Blood Transfusion Services Tissues and Cells Directorate (TCD) currently bank whole, frozen femoral head bone from living donors who are undergoing primary hip replacement surgery. When required, the bone is issued to a surgeon still frozen on dry ice (- 79 °C). Consequently, the femoral head bone is not processed, is not sterilised and at the time of issue, it contains donor blood, bone marrow and associated cells. We have previously shown that, cut, shaped and washed bone from deceased donors can be processed to remove up to 99...
October 11, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28952000/the-efficacy-and-sterilisation-of-human-decellularised-dermal-allografts-with-combinations-of-cupric-ions-and-hydrogen-peroxide
#12
S F Leow-Dyke, P Rooney, J N Kearney
Decellularised tissue allografts have been used in reconstructive surgical applications and transplantation for many years. Some of the current methods of sterilisation have a detrimental effect on the tissue graft structure and function. The anti-microbial activity of cupric ions and hydrogen peroxide (H2O2) are well known however their combined application is not currently utilised as a decontamination agent in the tissue banking world sector. The aim of this study was to determine the combined concentrations of copper chloride (CuCl2) and H2O2 that have the optimal bactericidal and sporicidal activity on decellularised (dCELL) human dermis...
September 26, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28916910/encompassing-atp-dna-insulin-and-protein-content-for-quantification-and-assessment-of-human-pancreatic-islets
#13
Meirigeng Qi, Shiela Bilbao, Elena Forouhar, Fouad Kandeel, Ismail H Al-Abdullah
Islet transplantation has made major progress to treat patients with type 1 diabetes. Islet mass and quality are critically important to ensure successful transplantation. Currently, islet status is evaluated using insulin secretion, oxygen consumption rate, or adenosine triphosphate (ATP) measurement. These parameters are evaluated independently and do not effectively predict islet status post-transplant. Therefore, assessing human pancreatic islets by encompassing ATP, DNA, insulin, and protein content from a single tissue sample would serve as a better predictor for islet status...
September 15, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28871437/free-radical-studies-of-components-of-the-extracellular-matrix-contributions-to-protection-of-biomolecules-and-biomaterials-from-sterilising-doses-of-ionising-radiation
#14
Barry J Parsons
The purpose of the current review is show how the principles and techniques of radiation chemistry have enabled the direct reactions of free radicals with biomolecules and biomaterials to be investigated at the molecular level. In particular, the review focusses on the free radical-induced fragmentation of glycosaminoglycans. Glycosaminoglycans are large linear polysaccharides consisting of repeating disaccharide units and are important components of the extracellular matrix (ECM) either in free form (hyaluronan) or as a component of proteoglycans...
September 4, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28799106/chemical-sterilization-of-allograft-dermal-tissues
#15
Abigail Phipps, Edward Vaynshteyn, John B Kowalski, Manh-Dan Ngo, Karen Merritt, Joel Osborne, Evangelia Chnari
Common terminal sterilization methods are known to alter the natural structure and properties of soft tissues. One approach to providing safe grafts with preserved biological properties is the combination of a validated chemical sterilization process followed by an aseptic packaging process. This combination of processes is an accepted method for production of sterile healthcare products as described in ANSI/AAMI ST67:2011. This article describes the validation of the peracetic acid and ethanol-based (PAAE) chemical sterilization process for allograft dermal tissues at the Musculoskeletal Transplant Foundation (MTF, Edison, NJ)...
December 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28825171/professor-glyn-o-phillip-s-legacy-within-the-iaea-programme-on-radiation-and-tissue-banking
#16
Jorge Morales Pedraza
Professor Phillips began his involvement in the implementation of this important IAEA programme, insisting that there were advantages to be gained by using the ionizing radiation technique to sterilize human and animal tissues, based on the IAEA experience gained in the sterilization of medical products. The outcome of the implementation of the IAEA programme on radiation and tissue banking demonstrated that Professor Phillips was right in his opinion.
August 19, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28823040/maternal-predictors-and-quality-of-umbilical-cord-blood-units
#17
Beata Bielec-Berek, Żaneta Jastrzębska-Stojko, Agnieszka Drosdzol-Cop, Cecylia Jendyk, Dariusz Boruczkowski, Tomasz Ołdak, Agnieszka Nowak-Brzezińska, Rafał Stojko
The aim of the study was to determine the relationship between the maternal age at delivery and selected properties of the cord blood stem cells. The study included 50 pregnant women aged between 18 and 38 years in which spontaneous labors or elective cesarean sections were performed. Umbilical cord blood was collected immediately after the women were delivered of newborns. The samples were analyzed in the Polish Stem Cells Bank in Warsaw. The highest mean WBC level (p < 0.05) was observed in the umbilical blood collected from patients aged 35 years and more...
August 19, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28821996/mesenchymal-stromal-stem-cell-separation-methods-concise-review
#18
REVIEW
Andreas Nicodemou, Lubos Danisovic
Mesenchymal stem (stromal) cells (MSCs) possess unique biological characteristics such as plasticity, long term self-renewal, secretion of various bioactive molecules and ability of active migration to the diseased tissues that make them unique tool for regenerative medicine, nowadays. Until now MSCs were successfully derived from many tissue sources including bone marrow, umbilical cord, adipose tissue, dental pulp etc. The crucial step prior to their in vitro expansion, banking or potential clinical application is their separation...
August 18, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28815373/autologous-nasal-chondrocytes-delivered-by-injectable-hydrogel-for-in-vivo-articular-cartilage-regeneration
#19
Wenliang Chen, Changhua Li, Maoxiu Peng, Bingju Xie, Lei Zhang, Xiaojun Tang
Cell based tissue engineering serves as a promising strategy for articular cartilage repair, which remains a challenge both for researchers and clinicians. The aim of this research was to assess the potential of autologous nasal chondrocytes (NCs) combined with alginate hydrogel as injectable constructs for rabbit articular cartilage repair. Autologous nasal chondrocytes were isolated from rabbit nasal septum, expanded either on monolayer or in 3D alginate hydrogel. In vitro, DNA quantification revealed that NCs can proliferate stable in 3D alginate matrix, but slower than that cultured in monolayer...
August 16, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28815364/long-bone-mesenchymal-stem-cells-lb-mscs-clinically-reliable-cells-for-osteo-diseases
#20
Shirin Toosi, Hojjat Naderi-Meshkin, Fatemeh Kalalinia, Mohammad Taghi Pievandi, Hossein Hosseinkhani, Ahmad Reza Bahrami, Asieh Heirani-Tabasi, Mahdi Mirahmadi, Javad Behravan
Mesenchymal stem cells (MSCs) have been designated as the most reliable cells in clinics to treat osteo-diseases because of their versatile nature. MSCs, isolated from long bone (Lb-MSCs) are rarely reported and named as RIA-MSCs because of the reamer-irrigator-aspirator (RIA) device. The potential of these cells in the treatment of non-union bone fractures made them the ideal candidates to be studied for clinical practices. In this work, effect of cryopreservation on the proliferation and differentiation capabilities of long bone MSCs (Lb-MSCs) has been studied...
August 16, 2017: Cell and Tissue Banking
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