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Cell and Tissue Banking

M L de Boom van Wonderen, J Blok Singerling, M Huijzer den Toom, D C Grootendorst, M Franken de Koster, C Brumsen
The number of tissue donations decreased in The Netherlands over recent years. The aim of this project was to determine the number of missed tissue donors in the Haaglanden Medical Centre and to develop a strategy to improve the number of tissue donors. We retrospectively analyzed patient files of all deceased patients in 2014 for their potential as tissue donors. Our objectives were to determine the number of missed tissue donors and the percentage of correctly identified tissue donors among all physicians and hospitalists in training...
November 16, 2018: Cell and Tissue Banking
Mohsen Eslami, Elham Zadeh Hashem, Abolfazl Ghaniei, Hossein Sayyah-Atashbeig
Sustainable reduction in semen quality due to detrimental effects of primary and secondary peroxidative products was occurred during liquid storage. The objective of the current experiment was to explore the influence of bovine serum albumin conjugated linoleic acid (LA) on the rooster spermatozoa routine tests and lipid peroxidative/antioxidative levels stored at 4 °C over 48 h. For this purpose, collected ejaculates (≥ 80% progressive motile spermatozoa) pooled and extended with the phosphate buffer medium without (control) or enriched with different amounts of LA (0...
November 16, 2018: Cell and Tissue Banking
Noor Rahman, Rafiullah Khan, Saeed Badshah
The bone auto grafting, isografting, allografting and xenografting are used for defective bone replacement or treatment in almost all living species. The X-ray and Gamma (electromagnetic radiation) sterilization performed on the donor bone graft to prevent toxicity or migration of virus/bacterial infections from donors to reciver. Conversely, X-ray and Gamma radiation deteriorates the bone mechanical properties and bone become more susceptible to fracture. Fracture toughness as well as other mechanical properties of bone change with these radiations...
November 13, 2018: Cell and Tissue Banking
Sharun Rajendran, Arthi Kirubhakaran, Rakshana Alaudheen, Kaavya Jayaramayya, Mahalakshmi Santhanakalai, Sanjeevagandhan Jayaraman, Sukumar Chinnaraju, Janardhana Kumar Reddy, Balachandar Vellingiri
Umbilical cord blood (UCB) is an important source of stem cells, the heart of regenerative medicine. As the globalization and population of the world continues to increase, we are faced with an inundation of new diseases, affecting millions of people. Research work considering stem cells is essential for developing therapy for various conditions. Reduced availability of UCB serves as a hindrance to promote further research. Hence, India being one of the most densely populated countries in the world, can be considered a potential UCB repository...
November 12, 2018: Cell and Tissue Banking
Sohrab Iranpour, Sajjad Rahimi Pordanjani, Siamak Sabour
No abstract text is available yet for this article.
November 12, 2018: Cell and Tissue Banking
Martha Holland, Rachel Cunningham, Lake Seymour, Katja Kleinsteuber, Amy Cunningham, Tara Patel, Michael Manos, Ryan Brennick, Jun Zhou, F Stephen Hodi, Mariano Severgnini
Peripheral blood mononuclear cells (PBMCs) are essential to the study of autoimmune, infectious, parasitic diseases, and cancer. In the rapidly growing field of cancer immunology, cellular phenotyping provides critical information about patient responses to treatments and treatment efficacies. Notably, the evaluation of T cell based therapies relies on the isolation of highly viable CD3+ T cell, CD4+ Helper T cell, and CD8+ Cytotoxic T cell populations before and during patient treatments. Cryopreservation of PBMC populations allows researchers to thaw and characterize clinical samples by flow cytometry, mass cytometry, sequencing, etc...
October 30, 2018: Cell and Tissue Banking
Seyed Ali Behruz Khaghani, Gunay Akbarova, Chin Fhong Soon, Gulrukh Dilbazi
Cytokines are extremely potent biomolecules that regulate cellular functions and play multiple roles in initiation and inhibition of disease. These highly specialised macromolecules are actively involved in control of cellular proliferation, apoptosis, cell migration and adhesion. This work, investigates the effect of transforming growth factor-beta2 (TGF-β2) on the biological regulation of chondrocyte and the repair of a created model wound on a multilayer culture system. Also the effect of this cytokine on cell length, proliferation, and cell adhesion has been investigated...
October 30, 2018: Cell and Tissue Banking
Elizaveta Padalko, Katrien Lagrou, Marie-Luce Delforge, Hilde Jansens, Nadine Ectors, Jean-Paul Pirnay, Johan Klykens, Etienne Sokal, Ludo Muylle, Agnes Libois, Alain Vanderkelen, Gilbert Verbeken, Conny Matthys, Dominique Goossens, Geert Hanssens, Muriel Baltes, Hilde Beele
The article Biological tests carried out on serum/plasma samples from donors of human body material for transplantation: Belgian experience and practical recommendations.
October 26, 2018: Cell and Tissue Banking
Cecylia Jendyk, Agnieszka Drosdzol-Cop, Agnieszka Nowak-Brzezińska, Marcin Sadłocha, Rafał Stojko, Żaneta Jastrzȩbska-Stojko
The aim of the study was to analyze the influence of oxygene partial pressure (p02), base excess (BE) and buffer base (BB) parameters of cord blood obtained perinatally on quantity of obtained cells and focus on the perfect donor criteria. The study included 50 pregnant women aged between 18 and 38 years in which spontaneous labors and elective cesarean sections were performed. Umbilical cord blood was collected immediately after the women were delivered of newborns. The cells were analyzed in the Polish Stem Cells Bank in Warsaw...
October 19, 2018: Cell and Tissue Banking
Dreyer Bester, Lezelle Botes, Johannes Jacobus van den Heever, Harry Kotze, Pascal Dohmen, Jose Luis Pomar, Francis Edwin Smit
Cryopreserved pulmonary homograft (CPH) implantation remains the gold standard for reconstruction of the right ventricular outflow tract (RVOT). Harvesting homografts < 24-h post mortem is the international norm, thereby largely excluding cadaveric donors. This study examines the structural integrity and stability of ovine pulmonary homografts harvested after a 48-h post mortem period, cryopreserved and then implanted for up to 180 days. Fifteen ovine pulmonary homografts were harvested 48-h post mortem and cryopreserved...
October 11, 2018: Cell and Tissue Banking
Nashwa K Radwan, N F Ibrahim, A S Bashandy
Psoriasis is a chronic inflammatory autoimmune disease with undefined etiology. It affects 2-3% of the worldwide population. The unsatisfactory outcome of variable treatments of the disease is partially due to the poor compliance of the present therapies with more or less side effects. As known, the human amniotic membrane is a popular intervention for many diseases. Amniotic membrane has been found recently to possess antimicrobial, anti-inflammatory and immunosuppressive properties. The irradiated amniotic membrane was used in this study as a topical application for 4 weeks on localized lesions in cases of mild psoriasis...
September 25, 2018: Cell and Tissue Banking
Daniel Trujillo Diaz, Nora C Hernandez, Etty P Cortes, Phyllis L Faust, Jean Paul G Vonsattel, Elan D Louis
A review of the brain banking literature reveals a primary focus either on the factors that influence the decision to become a future donor or on the brain tissue processing that takes place after the individual has died (i.e., the front-end or back-end processes). What has not been sufficiently detailed, however, is the complex and involved process that takes place after this decision to become a future donor is made yet before post-mortem processing occurs (i.e., the large middle-ground). This generally represents a period of many years during which the brain bank is actively engaged with donors to ensure that valuable clinical information is prospectively collected and that their donation is eventually completed...
September 15, 2018: Cell and Tissue Banking
Giulia Montagner, Diletta Trojan, Elisa Cogliati, Flavio Manea, Andrea Vantini, Adolfo Paolin
Although careful donor selection reduces tissue contamination, close microbiological control of harvested allografts remains a key task of tissue banks. To guarantee the safety of human tissues for allograft transplantation, a decontamination regimen must be adopted which, as recommended by European guidelines, is active against the majority of microorganisms isolated in tissues. Antibiotic decontamination methods differ from one tissue bank to another in terms of antimicrobial agents, temperature and length of exposure...
September 11, 2018: Cell and Tissue Banking
M J Eagle, J Man, P Rooney, J N Kearney
NHS Blood and Transplant Tissue and Eye Services banks and issues, cut, shaped and washed bone from deceased donors. The bone is cut/shaped prior to washing and then processed to remove up to 99.9% of blood, bone marrow and associated cells. The processed bone is then sterilised by gamma irradiation with or without a freeze-drying step. Removal of donor blood and bone marrow has been reported to aid incorporation of allograft bone without affecting the biomechanical properties of the bone. However, cut and shaped bone is not suitable for some orthopaedic procedures and some orthopaedic surgeons do not wish to use irradiated bone...
September 11, 2018: Cell and Tissue Banking
Abhinav Golla, Li Wang, Caleb Morris, Mitchell P Weikert, Sumitra S Khandelwal
To evaluate the repeatability of central corneal thickness (CCT) measurements in donor corneas using optical coherence tomography (OCT, RTVue-Optovue, Inc., Fremont, CA). Consecutive corneas were measured by a single observer using the RTVue. All corneas were preserved in the Transend chamber and Life4 °C media (Numedis, Inc., Isanti, MN/USA) and stored at 4 °C. The repeatability was evaluated using a pooled within-subject standard deviation (SD), coefficient of variation (CoV), and intraclass correlation coefficient (ICC)...
September 4, 2018: Cell and Tissue Banking
Zhiguo He, Julie Gueudry, David Toubeau, Philippe Gain, Gilles Thuret, Marc Muraine
Our aim was to measure the endothelial quality of prestripped Descemet membrane endothelial keratoplasty (DMEK) 48 h after preparation in an eye bank with the Muraine technique and shipping in a distant center. Ten pairs of human corneas with similar eye bank endothelial cell density (ebECD) were stored in organ-culture (OC) for 25 days (20, 28) [median (10-90 percentiles)]. One cornea was then randomized to DMEK preparation using the Moria Muraine trephine, the other served as control. The grafts were left attached to the center of the cornea, immersed in the OC medium (without Dextran) and shipped to a distant center...
August 31, 2018: Cell and Tissue Banking
Noëmi M C De Roo, Laurent A M Thierens, Liesbeth Temmerman, Elise Dekeyser, Jerina Boelens, Guy A M De Pauw
Autotransplantation is a versatile technique for the replacement of a missing tooth and cryopreservation can expand its scope. The aim of this in vitro study is to compare the antimicrobial effect of different transport protocols on procured teeth prior to cryopreservation. Streptococcus oralis biofilms were grown on ten sterile premolars, incubated for 48 h and subjected to the following transport procedures: an untreated (contaminated) control group, a group rinsed with phosphate buffered saline (PBS), a group transported in PBS, a group transported in Dulbecco's Modified Eagle's medium (DMEM) supplemented with fetal calf serum (FCS), and a group transported in DMEM supplemented with FCS and antibiotics (AB)...
August 29, 2018: Cell and Tissue Banking
Elizaveta Padalko, Katrien Lagrou, Marie-Luce Delforge, Hilde Jansens, Nadine Ectors, Jean-Paul Pirnay, Johan Klykens, Etienne Sokal, Ludo Muylle, Agnes Libois, Alain Vanderkelen, Gilbert Verbeken, Conny Matthys, Dominique Goossens, Geert Hanssens, Muriel Baltes, Hilde Beele
This paper on the biological tests carried out on serum/plasma samples from donors of human body material (HBM) is the result of a project of the working Group of Superior Health Council of Belgium formed with experts in the field of HBM and infectious serology. Indeed, uncertainty about the interpretation of biological test results currently leads to the sometimes unjustified cancelling of planned donations or the rejection of harvested HBM, whilst more sophisticated diagnostic algorithms would still allow the use of organs or HBM that would otherwise have been rejected...
August 29, 2018: Cell and Tissue Banking
Luz María Ruíz-Godoy, Fabiola Berenice Baez-Revueltas, María de Lourdes Suarez-Roa, Héctor Maldonado-Martínez, Virginia Enriquez-Carcamo, Ana Laura Colín-González, Abelardo Meneses-García
In this work we estimated the budgetary impact of the samples produced by the biobank of the "Instituto Nacional de Cancerología" (BT-INCan) to set a recuperation fee from the perspective of the Health Ministry of Mexico. The study is an observational retrospective review of the direct medical costs (DMCs) of the processes involved in cryopreservation of the samples collected, on a per sample basis, including materials, laboratory tests, personnel, and administrative costs. Materials and labor costs were determined by information collected from the BT-INCan...
August 28, 2018: Cell and Tissue Banking
Letícia Kraft, Victoria Stadler Tasca Ribeiro, Luciana Cristina Wollmann, Paula Hansen Suss, Felipe Francisco Tuon
All cardiac allograft tissues are under potential contamination, requiring a validated terminal sterilization process or a minimal bioburden. The bioburden calculation is important to determine the bacterial burden and further decontamination and disinfection strategies for the valve processing. The aim of this study was to determine the bioburden from transport solution (TS) of heart valves obtained from non-heart-beating and heart-beating donors in different culture methods. The bioburden from TS was determined in 20 hearts donated for valve allograft tissue using membrane filter (MF) and direct inoculation...
September 2018: Cell and Tissue Banking
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