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Cell and Tissue Banking

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https://www.readbyqxmd.com/read/29728941/regulatory-aspects-of-tissue-donation-banking-and-transplantation-in-india
#1
Astrid Lobo Gajiwala
Amendments to India's Transplantation of Human Organs Act, 1994, have established the legality of tissue donation and transplantation from deceased donors and the conditions under which they are permitted. The amended Act, now known as The Transplantation of Human Organs and Tissues Act, 1994, seeks to prevent the commercialization of tissue donation and to guarantee the safety of indigenous allografts. Registration of tissue banks, compliance with national standards and the appointment of transplant co-ordinators in hospitals registered under the Act are now mandatory...
May 4, 2018: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29700649/the-addition-of-albumin-improves-schwann-cells-viability-in-nerve-cryopreservation
#2
Sara Alicia González Porto, Nieves Domenech, Alba González Rodríguez, Edgar Mauricio Avellaneda Oviedo, Francisco J Blanco, María C Arufe Gonda, Ángel Álvarez Jorge, Jacinto Sánchez Ibañez, Esther Rendal Vázquez
The purpose of the current study was to establish a valid protocol for nerve cryopreservation, and to evaluate if the addition of albumin supposed any advantage in the procedure. We compared a traditional cryopreservation method that uses dimethyl sulfoxide (DMSO) as cryoprotectant, to an alternative method that uses DMSO and albumin. Six Wistar Lewis rats were used to obtain twelve 20 mm fragments of sciatic nerve. In the first group, six fragments were cryopreserved in 199 media with 10% DMSO, with a temperature decreasing rate of 1 °C per minute...
April 26, 2018: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29696490/interaction-of-preservation-methods-and-radiation-sterilization-in-human-skin-processing-with-particular-insight-on-the-impact-of-the-final-water-content-and-collagen-disruption-part-i-process-validation-water-activity-and-collagen-changes-in-tissues-cryopreserved
#3
M R Herson, K Hamilton, J White, D Alexander, S Poniatowski, A J O'Connor, J A Werkmeister
Current regulatory requirements demand an in-depth understanding and validation of protocols used in tissue banking. The aim of this work was to characterize the quality of split thickness skin allografts cryopreserved or manufactured using highly concentrated solutions of glycerol (50, 85 or 98%), where tissue water activity (aw ), histology and birefringence changes were chosen as parameters. Consistent aw outcomes validated the proposed processing protocols. While no significant changes in tissue quality were observed under bright-field microscopy or in collagen birefringence, in-process findings can be harnessed to fine-tune and optimize manufacturing outcomes in particular when further radiation sterilization is considered...
April 25, 2018: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29696489/tissue-banking-in-asia-pacific-region-past-present-and-future
#4
Aziz Nather, Foong Shi Yun Mandy, Tan Ning, Wang Kaiying
Tissue banking in the Asia Pacific regions is driven by two main forces-firstly the International Atomic Energy Agency (IAEA) via Regional Co-operative Agreement projects and secondly by the Asia Pacific Association of Surgical Tissue Banking (APASTB). This overview is written in three sections: (1) History of tissue banking in individual country in the region. (2) History of APASTB. (3) History of IAEA programme in Asia Pacific region. The current status and future of the tissue banking programme in the region will be discussed...
April 25, 2018: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29693226/effect-of-freezing-and-storage-temperature-on-stability-and-antimicrobial-activity-of-an-antibiotic-mixture-used-for-decontamination-of-tissue-allografts
#5
Vicente Mirabet, Ana Melero, M Dolores Ocete, Dimitra Bompou, Miriam Torrecillas, Juan J Carreras, Isabel Valero, Ana I Marqués, Rafael Medina, Luis R Larrea, Cristina Arbona, Teresa M Garrigues, Concepción Gimeno
One of the most important risks to be controlled in tissue banking is the infection associated with the clinical use of auto- and allografts. Thus, tissue disinfection protocols are used, in addition to processing in controlled environments. For this purpose, combinations of antibiotics are designed to ensure a broad spectrum of antimicrobial activity. This type of protocol is usually validated by testing its antimicrobial efficacy. In this work, we have studied the effect of several factors on the potential of an antibiotic mixture: container, freezing, storage at 4 °C, storage at - 30 °C and storage at - 80 °C...
April 24, 2018: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29687168/comparison-of-automated-and-conventional-microbiological-examination-of-donated-human-cardiac-tissue-in-heart-valve-banking
#6
Letícia Kraft, Felipe Francisco Tuon, Victoria Stadler Tasca Ribeiro, Luciana Cristina Wollmann, Paula Hansen Suss, Marcelo Pilonetto, Francisco Diniz Affonso da Costa
Most tissue banks use the conventional method; however, the automated method has advantages over the conventional method. The aim of this study was to compare the conventional and automated methods of culture in human cardiac tissue using an artificial contamination model. Myocardial samples were contaminated with sequential concentration (104 to 10-1  CFU/mL) with Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and Candida albicans. Cultures were obtained from solution were the fragment was immersed and minced tissue, before and after the routine decellularization solution, with automated and conventional culture methods...
April 23, 2018: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29556882/bioburden-in-transport-solutions-of-human-cardiovascular-tissues-a-comparative-evaluation-of-direct-inoculation-and-membrane-filter-technique
#7
Letícia Kraft, Victoria Stadler Tasca Ribeiro, Luciana Cristina Wollmann, Paula Hansen Suss, Felipe Francisco Tuon
All cardiac allograft tissues are under potential contamination, requiring a validated terminal sterilization process or a minimal bioburden. The bioburden calculation is important to determine the bacterial burden and further decontamination and disinfection strategies for the valve processing. The aim of this study was to determine the bioburden from transport solution (TS) of heart valves obtained from non-heart-beating and heart-beating donors in different culture methods. The bioburden from TS was determined in 20 hearts donated for valve allograft tissue using membrane filter (MF) and direct inoculation...
March 19, 2018: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29511942/the-evaluation-of-the-transport-medium-for-extracted-premolars-prior-to-cryopreservation-a-systematic-literature-review
#8
REVIEW
Noëmi M C De Roo, Laurent A M Thierens, Liesbeth Temmerman, Guy A M De Pauw
Prior to cryopreservation, a tooth is transported from a contaminated oral environment to the tooth bank. Our objective was to identify all studies reporting or investigating a transport protocol prior to the cryopreservation of teeth, in terms of decontamination of the subjects. The systematic literature search (1970-2017) was based on MEDLINE via PubMed, Web of Science and the Cochrane Library. The reference lists of the included studies and the Science Citation Index were used for hand searching (snowballing)...
March 7, 2018: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29508105/decellularized-amnion-scaffold-with-activated-prp-a-new-paradigm-dressing-material-for-burn-wound-healing
#9
Jeevitaa Kshersagar, Ravi Kshirsagar, Shashikant Desai, Raghvendra Bohara, Meghnad Joshi
Direct application of amnion has greater risk of immunological rejection and infection. Decellularization is an effective method to lower the risk of immune complications and infections. The bioreactor assembly with multiple cassettes was designed for decellurization of multiple amnions with different cell types simultaneously in single run. A detergent-based protocol was modified to remove all cellular components from amnion and diminish the DNA content to render it non-immunogenic. Amnion (n = 10) were treated with 2% sodium dodecyl sulphate (SDS), 5% dimethyl sulfoxide (DMSO) and 2% sodium deoxycholeate (SD)...
March 5, 2018: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29502254/organization-model-for-allotransplantations-of-cryopreserved-vascular-grafts-in-czech-republic
#10
Miroslav Špaček, Pavel Měřička, Libor Janoušek, Petr Štádler, Miloš Adamec, Robert Vlachovský, Igor Guňka, Pavel Navrátil, Filip Thieme, Petr Mitáš, Rudolf Špunda, Jaroslav Špatenka, Robert Staffa, Petr Němec, Jaroslav Lindner
The transplantation of fresh or cryopreserved vascular allografts in patients with a prosthetic graft infection or critical limb ischemia is necessary for their limb salvage and, in many cases, represents a lifesaving procedure. While transplantation of fresh allografts has a long history in the Czech Republic, the standard use of cryopreserved vascular allografts was introduced into the clinical practice in 2011 as a result of the implementation of EU Directive 2004/23/EC into national legislation (Human Cell and Tissue Act No...
March 3, 2018: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29075945/use-of-freeze-dried-bone-allografts-in-osteoporotic-patients-undergoing-median-sternotomy
#11
Mei Liang, Jianzhou Liu, Qi Miao, Guotao Ma, Xingrong Liu, Xiaofeng Li, Chaoji Zhang
Osteoporosis is a major risk factor for deep sternal wound infection, which is a rare but serious complication after median sternotomy. We investigated the incidence of deep sternal wound infection and the protective effect of bone allografts in osteoporotic patients after sternal approximation. Data were collected retrospectively from consecutive osteoporotic patients who underwent cardiac surgery via median sternotomy. Sternal approximation in the historical control group was performed with conventional steel wire sutures...
March 2018: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29071453/donor-cornea-tissue-in-cases-of-drowning-or-water-submersion-eye-banks-practice-patterns-and-tissue-outcomes
#12
Nithya P Vijayakumar, Purak Parikh, Shahzad I Mian, Brad Tennant, Gregory H Grossman, Bob Albrecht, Leslie M Niziol, Maria A Woodward
Surgical use of donor corneal tissue from victims of water submersion (drowning or submersion secondary to death) remains controversial due to limited evidence about the quality of these tissues. To assess the safety of donor corneal tissue from victims of water submersion, an investigation of eye banks' practice patterns and tissue outcomes was conducted. All 79 Eye Bank Association of America accredited eye banks were contacted for a phone interview of practices regarding tissue from victims of water submersion...
March 2018: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29043524/improving-the-success-rate-of-human-corneal-endothelial-cell-cultures-from-single-donor-corneas-with-stabilization-medium
#13
D Spinozzi, A Miron, M Bruinsma, J T Lie, I Dapena, S Oellerich, G R J Melles
Main objective of this study was to improve the success rate of human corneal endothelial cell (hCEC) cultures from single donor corneas. We could show that the use of stabilization medium prior to cell isolation may have a positive effect on the success rate of hCEC cultures from single research-grade donor corneas by allowing growth of otherwise possibly not successful cultures and by improving their proliferative rate. hCEC were obtained from corneo-scleral rims of 7 discarded human research-grade cornea pairs...
March 2018: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29043522/cryovial-monolayer-vitrification-for-ovarian-tissue-cryopreservation
#14
Hongxia Li, Haixia Cao, Xingping Guo, Huaixiu Wang
The cryopreservation of ovarian tissue has been proved to be effective for fertility preservation. To find a better cryopreservation method, we tested the efficacy of cryovial monolayer vitrification method in comparison with that of needle immersed vitrification (NIV). Ovaries from 10 female kunming mice aged 6-8 weeks were cut into pieces and allocated into group A (cryovial monolayer vitrification method), group B (NIV method) and group C (fresh control). In group A, pieces of ovarian tissue were layered around the inner wall of cryovial as a monolayer; and in group B, pieces of ovarian tissue were pierced with a needle...
March 2018: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29039070/the-evaluation-of-xenotransplantation-of-feline-ovarian-tissue-vitrified-by-needle-immersed-vitrification-technique-into-male-immunodeficient-mice
#15
Mürşide Ayşe Demirel, Duygu Baki Acar, Burcu Ekim, Ferda Topal Çelikkan, Kübra Karakaş Alkan, Seçkin Salar, Esra Atabenli Erdemli, Sinan Özkavukçu, Seda Sağlam Yar, Halit Kanca, Ayhan Baştan
In this study, the efficiency of the "Needle Immersed Vitrification" technique was tested on cryopreserved feline ovarian tissue. For vitrification, ovarian fragments (0.5-1.5 mm2 ) from each ovary were collected; the grafts were exposed to 7.5-15% ethylene glycol and 7.5-15% dimethyl sulfoxide at room temperature and stored in liquid nitrogen at least 1 week. Morphologic examinations, expression of genes such as B cell lymphoma 2, B-cell lymphoma-2-associated X protein, Bone morphogenetic protein 15, zone of polarizing activity, zona pellucida C protein and DNA (cytosine-5)-methyltransferase 1, ultrastructural analysis and viability tests were carried out from collected grafts...
March 2018: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29027064/light-scattering-measurements-in-electron-beam-sterilized-corneas-stored-in-recombinant-human-serum-albumin
#16
Khoa D Tran, Yan Li, Jeffrey D Holiman, Maolong Tang, David Huang, Michael D Straiko, Christopher G Stoeger
Irradiated corneal tissues have been used for a variety of ophthalmic procedures including glaucoma drainage device covers and lamellar grafts. The maintenance of corneal clarity is important, as light obstructions resulting from processing or long-term storage of irradiated corneas may negatively affect vision and postoperative cosmesis. It has been reported that corneal tissues can be preserved in human serum albumin (HSA), however, the clarity of corneas after long-term storage in HSA has not been well described...
March 2018: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28916910/encompassing-atp-dna-insulin-and-protein-content-for-quantification-and-assessment-of-human-pancreatic-islets
#17
Meirigeng Qi, Shiela Bilbao, Elena Forouhar, Fouad Kandeel, Ismail H Al-Abdullah
Islet transplantation has made major progress to treat patients with type 1 diabetes. Islet mass and quality are critically important to ensure successful transplantation. Currently, islet status is evaluated using insulin secretion, oxygen consumption rate, or adenosine triphosphate (ATP) measurement. These parameters are evaluated independently and do not effectively predict islet status post-transplant. Therefore, assessing human pancreatic islets by encompassing ATP, DNA, insulin, and protein content from a single tissue sample would serve as a better predictor for islet status...
March 2018: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28823040/maternal-predictors-and-quality-of-umbilical-cord-blood-units
#18
Beata Bielec-Berek, Żaneta Jastrzębska-Stojko, Agnieszka Drosdzol-Cop, Cecylia Jendyk, Dariusz Boruczkowski, Tomasz Ołdak, Agnieszka Nowak-Brzezińska, Rafał Stojko
The aim of the study was to determine the relationship between the maternal age at delivery and selected properties of the cord blood stem cells. The study included 50 pregnant women aged between 18 and 38 years in which spontaneous labors or elective cesarean sections were performed. Umbilical cord blood was collected immediately after the women were delivered of newborns. The samples were analyzed in the Polish Stem Cells Bank in Warsaw. The highest mean WBC level (p < 0.05) was observed in the umbilical blood collected from patients aged 35 years and more...
March 2018: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28815373/autologous-nasal-chondrocytes-delivered-by-injectable-hydrogel-for-in-vivo-articular-cartilage-regeneration
#19
Wenliang Chen, Changhua Li, Maoxiu Peng, Bingju Xie, Lei Zhang, Xiaojun Tang
Cell based tissue engineering serves as a promising strategy for articular cartilage repair, which remains a challenge both for researchers and clinicians. The aim of this research was to assess the potential of autologous nasal chondrocytes (NCs) combined with alginate hydrogel as injectable constructs for rabbit articular cartilage repair. Autologous nasal chondrocytes were isolated from rabbit nasal septum, expanded either on monolayer or in 3D alginate hydrogel. In vitro, DNA quantification revealed that NCs can proliferate stable in 3D alginate matrix, but slower than that cultured in monolayer...
March 2018: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28812172/delayed-post-mastectomy-breast-reconstructions-with-allogeneic-acellular-dermal-matrix-prepared-by-a-new-decellularizationmethod
#20
Martin Bohac, Ivan Varga, Stefan Polak, Jana Dragunova, Jozef Fedeles, Jan Koller
Acellular dermal matrix (ADM) is a tissue graft of allogeneic origin from post-mortem tissue donors prepared by an innovative decellularization process. The newly developed non-toxic and low cost decellularization process of cadaver origin dermis included ADM in breast reconstruction procedures proved to help coverage of the lower-pole of breast expanders or implants. As the results have shown, it did help to eliminate autologous dermis donor site morbidity along with shortening the operation time by avoiding elevation of additional muscle or fascia during the operation...
March 2018: Cell and Tissue Banking
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