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Cell and Tissue Banking

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https://www.readbyqxmd.com/read/28275875/rapid-preparation-of-a-noncultured-skin-cell-suspension-that-promotes-wound-healing
#1
Cheonjae Yoon, Jungsuk Lee, Hyosun Jeong, Sungjun Lee, Taesik Sohn, Sungphil Chung
Autologous skin cell suspensions have been used for wound healing in patients with burns and against normal pigmentation in vitiligo. To separate cells and the extracellular matrix from skin tissue, most researchers use enzymatic digestion. Therefore, this process is difficult to perform during a routine surgical procedure. We aimed to prepare a suspension of noncultured autologous skin cells (NCSCs) using a tissue homogenizer as a new method instead of harsh biochemical reagents. The potential clinical applicability of NCSCs was analyzed using a nude-rat model of burn healing...
March 8, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28258397/multicenter-fresh-frozen-tissue-sampling-in-colorectal-cancer-does-the-quality-meet-the-standards-for-state-of-the-art-biomarker-research
#2
Z S Lalmahomed, R R J Coebergh van den Braak, M H A Oomen, S P Arshad, P H J Riegman, J N M IJzermans
The growing interest in the molecular subclassification of colorectal cancers is increasingly facilitated by large multicenter biobanking initiatives. The quality of tissue sampling is pivotal for successful translational research. This study shows the quality of fresh frozen tissue sampling within a multicenter cohort study for colorectal cancer (CRC) patients. Each of the seven participating hospitals randomly contributed ten tissue samples, which were collected following Standard Operating Procedures (SOP) using established techniques...
March 3, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28255773/cell-lines-authentication-and-mycoplasma-detection-as-minimun-quality-control-of-cell-lines-in-biobanking
#3
C Corral-Vázquez, R Aguilar-Quesada, P Catalina, G Lucena-Aguilar, G Ligero, B Miranda, J A Carrillo-Ávila
Establishment of continuous cell lines from human normal and tumor tissues is an extended and useful methodology for molecular characterization of cancer pathophysiology and drug development in research laboratories. The exchange of these cell lines between different labs is a common practice that can compromise assays reliability due to contamination with microorganism such as mycoplasma or cells from different flasks that compromise experiment reproducibility and reliability. Great proportions of cell lines are contaminated with mycoplasma and/or are replaced by cells derived for a different origin during processing or distribution process...
March 2, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28255772/isolation-culture-and-biological-characteristics-of-multipotent-porcine-skeletal-muscle-satellite-cells
#4
Jinjuan Yang, Hao Liu, Kunfu Wang, Lu Li, Hongyi Yuan, Xueting Liu, Yingjie Liu, Weijun Guan
Skeletal muscle has a huge regenerative potential for postnatal muscle growth and repair, which mainly depends on a kind of muscle progenitor cell population, called satellite cell. Nowadays, the majority of satellite cells were obtained from human, mouse, rat and other animals but rarely from pig. In this article, the porcine skeletal muscle satellite cells were isolated and cultured in vitro. The expression of surface markers of satellite cells was detected by immunofluorescence and RT-PCR assays. The differentiation capacity was assessed by inducing satellite cells into adipocytes, myoblasts and osteoblasts...
March 2, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28255771/amniotic-membrane-in-ophthalmology-properties-preparation-storage-and-indications-for-grafting-a-review
#5
REVIEW
Katerina Jirsova, Gary L A Jones
The use of amniotic membrane in ophthalmic surgery and other surgical procedures in the fields of dermatology, plastic surgery, genitourinary medicine and otolaryngology is on the increase. Furthermore, amniotic membrane and its epithelial and mesenchymal cells have broad use in regenerative medicine and hold great promise in anticancer treatment. Amniotic membrane is a rich source of biologically active factors and as such, promotes healing and acts as an effective material for wound dressing. Amniotic membrane supports epithelialization and exhibits anti-fibrotic, anti-inflammatory, anti-angiogenic and anti-microbial features...
March 2, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28238108/enhanced-vascular-biocompatibility-of-decellularized-xeno-allogeneic-matrices-in-a-rodent-model
#6
M van Steenberghe, T Schubert, Y Guiot, C Bouzin, X Bollen, P Gianello
Glutaraldehyde preservation is the gold standard for cardiovascular biological prosthesis. However, secondary calcifications and the absence of tissue growth remain major limitations. Our study assessed in vitro and in vivo the biocompatibility of human (fascia lata, pericardium) and porcine tissues (pericardium, peritoneum) treated with a physicochemical procedure for decellularization and non-conventional pathogens inactivation. Biopsies were performed before and after treatment to assess decellularization (HE/Dapi staining/DNA quantification/MHC I/alpha gal immunostaining) and mechanical integrity...
February 25, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28236164/evaluation-of-the-effects-of-the-combination-of-autologous-mesenchymal-stem-cells-and-platelet-rich-plasma-on-structural-bone-allograft-healing
#7
Chang-Goo Park, Min Wook Joo, Jinyoung Jeong, Yong-Koo Kang, Da-Reum Lee
In this study, we evaluated the efficacy of the combination of autologous mesenchymal stem cells (MSCs) and platelet-rich plasma (PRP) for enhancing structural allogenic bone graft healing in rabbits. For these experiments segmental bone defects (1.5-2 cm) were generated on femoral diaphysis of New Zealand white rabbits and reconstructed via structural allogenic bone grafting and additional intramedullary nail fixation. The structural allografts were subsequently wrapped with Gelfoam containing autologous MSCs and PRP, or PRP alone (control)...
February 24, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28233169/can-stem-cells-enhance-bone-formation-in-the-human-edentulous-alveolar-ridge-a-systematic-review-and-meta-analysis
#8
REVIEW
Lucyene Miguita, Andrea Mantesso, Claudio Mendes Pannuti, Maria Cristina Zindel Deboni
Several non-biological materials are currently being used to increase the alveolar bone volume to support dental implants. Recently, stem cell therapy has emerged as a promising biological substitute or adjuvant to enhance bone healing. In order to determine if stem cell therapy has enough clinical evidence to bone ridge augmentation in humans, a systematic review and meta-analysis were conducted. Two independent investigators searched the Entrez PubMed, SCOPUS and Web of Science databases for eligible randomized clinical trials that describe stem cell therapies for alveolar bone formation...
February 23, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28138805/umbilical-cord-derived-mesenchymal-stem-cell-transplantation-for-treating-elderly-vascular-dementia
#9
Yan He, Xingfang Jin, Jun Wang, Mingyao Meng, Zongliu Hou, Weimeng Tian, You Li, Wenju Wang, Yunhong Wei, Yipeng Wang, Hongqiong Meng, Xuyang Lu, Zhuo Chen, Linyao Fu
This study aimed to determine the efficacy and safety of human umbilical cord-derived mesenchymal stem cell (HUC-MSC) transplantation for treating elderly vascular dementia (VaD). Ten VaD patients (average age, 73.88 years old) were treated. HUC-MSCs were isolated, cultured, stem cell-marked, and qualified and administered as a 3-course intravenous infusion to these patients. The Mini-Mental State Exam (MMSE) and the Activities of Daily Living Index (Barthel Index scoring system) were used to assess the cognitive function and daily living activity improvements in these patients before transplantation (T0), 3 months after transplantation (T1), and 6 months after transplantation (T2)...
January 30, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28058525/the-visual-assessment-of-broth-cultures-for-tissue-bank-samples
#10
Kerry Varettas
The bioburden screening process of allograft musculoskeletal tissue samples received at the South Eastern Area Laboratory Services includes the routine use of solid agar and cooked meat (CM) broth media. CM has been routinely sub-cultured onto solid agar plates after aerobic incubation at 35 °C. This study will evaluate whether a visual assessment of CM can replace sub-culture by an in vitro inoculation and a prospective study. Eight challenge organisms were serially diluted and inoculated into CM. The average inoculum of 0...
January 5, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28058524/determination-of-residual-dimethylsulfoxide-in-cryopreserved-cardiovascular-allografts
#11
R Díaz Rodríguez, B Van Hoeck, S De Gelas, F Blancke, R Ngakam, K Bogaerts, R Jashari
Dimethylsulfoxide (DMSO) is a solvent which protects the structure of allografts during the cryopreservation and thawing process. However, several toxic effects of DMSO in patients after transplantation of cryopreserved allografts have been described. The aim of this study is to determine the residual DMSO in the cardiovascular allografts after thawing and preparation of cryopreserved allografts for clinical application following guidelines of the European Pharmacopoeia for DMSO detection. Four types of EHB allografts (aortic valve-AV, pulmonary valve-PV, descending thoracic aorta-DA, and femoral artery-FA) are cryopreserved using as cryoprotecting solution a 10% of DMSO in medium 199...
January 5, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28116630/corneal-transplantation-study-of-the-data-of-a-regional-eye-bank-for-the-year-2013-and-analysis-of-the-evolution-of-the-adverse-events-reported-in-france-since-2010
#12
Anne-Sophie Gauthier, Marie Castelbou, Mélanie Bidaut Garnier, Joëlle Pizzuto, Stephan Roux, Philippe Gain, Fabienne Pouthier, Bernard Delbosc
To analyze the data of the adverse events collected in a single major eye bank (EFS Bourgogne Franche Comté, Besançon, France) for the year 2013 and to report the French data of biovigilance provided by the French National Agency for Medicines and Health Products Safety (ANSM) between 2010 and 2013. we have set up a study of adverse events in 2013, in collaboration with a single eye bank (EFS Bourgogne Franche Comté, Besançon, France). A survey was sent to the surgeon for each delivered corneal button by the eye bank in 2013...
March 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28004288/in-vivo-quantification-of-the-revascularization-of-a-human-acellular-dermis-seeded-with-epcs-and-mscs-in-co-culture-with-fibroblasts-and-pericytes-in-the-dorsal-chamber-model-in-pre-irradiated-tissue
#13
M Vitacolonna, D Belharazem, P Hohenberger, E D Roessner
INTRODUCTION: Transplantation of a cell-seeded graft may improve wound healing after radiotherapy. However, the survival of the seeded cells depends on a rapid vascularization of the graft. Co-culturing of adult stem cells may be a promising strategy to accelerate the vessel formation inside the graft. Thus, we compared the in vivo angiogenic potency of mesenchymal stem cells (MSC) and endothelial progenitor cells (EPC) using dorsal skinfold chambers and intravital microscopy. MATERIALS AND METHODS: Cells were isolated from rat bone marrow and adipose tissue and characterized by immunostaining and flow cytometry...
March 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/27999996/fresh-and-in-vitro-osteodifferentiated-human-amniotic-membrane-alone-or-associated-with-an-additional-scaffold-does-not-induce-ectopic-bone-formation-in-balb-c-mice
#14
Romain Laurent, Aurélie Nallet, Benoit de Billy, Laurent Obert, Laurence Nicod, Christophe Meyer, Pierre Layrolle, Narcisse Zwetyenga, Florelle Gindraux
The human amniotic membrane (hAM) has been successfully used as a natural carrier containing amniotic mesenchymal stromal cells, epithelial cells and growth factors. It has a little or no immunogenicity, and possesses useful anti-microbial, anti-inflammatory, anti-fibrotic and analgesic properties. It has been used for many years in several indications for soft tissue repair. We previously reported that hAM represents a natural and preformed sheet containing highly potent stem cells, and could thus be used for bone repair...
March 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/27928705/semi-quantitative-assessments-of-dextran-toxicity-on-corneal-endothelium-conceptual-design-of-a-predictive-algorithm
#15
Filip Filev, Ceprail Oezcan, Jana Feuerstacke, Stephan J Linke, Birgit Wulff, Olaf J C Hellwinkel
Dextran is added to corneal culture medium for at least 8 h prior to transplantation to ensure that the cornea is osmotically dehydrated. It is presumed that dextran has a certain toxic effect on corneal endothelium but the degree and the kinetics of this effect have not been quantified so far. We consider that such data regarding the toxicity of dextran on the corneal endothelium could have an impact on scheduling and logistics of corneal preparation in eye banking. In retrospective statistic analyses, we compared the progress of corneal endothelium (endothelium cell loss per day) of 1334 organ-cultured corneal explants in media with and without dextran...
March 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/27900507/-why-throw-away-something-useful-attitudes-and-opinions-of-people-treated-for-bipolar-disorder-and-their-relatives-on-organ-and-tissue-donation
#16
Carolina S Padoan, Lucas F Garcia, Aline A Rodrigues, Lucas M Patusco, Mariana V Atz, Flavio Kapczinski, José R Goldim, Pedro V S Magalhães
In regard to mental illness, brain donation is essential for the biological investigation of central pathology. Nevertheless, little is known about the thoughts of people with mental disorders on tissue donation for research. Here, our objective was to understand the attitudes and opinions of people treated for bipolar disorder and their relatives regarding donation in general, and particularly donation for research. This is a qualitative study that used in-depth interviews to determine the thoughts of participants regarding tissue donation for research...
March 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/27878655/the-use-of-donor-scleral-patch-in-ophthalmic-surgery
#17
REVIEW
Christopher Hodge, Gerard Sutton, Raj Devasahayam, Pierre Georges, Jane Treloggen, Simon Cooper, Con Petsoglou
Scleral tissue has been in use in ophthalmology for many years although indications for use have varied. We retrospectively reviewed scleral transplant tissue requests over a 12 month period at a local eye bank and confirmed a small but significant demand for the use of scleral tissue. Iatrogenic surgical complications are the primary indication for use. Our understanding of the indications and outcomes of scleral graft procedures is derived from case reports and small cohort series. We reviewed the current literature on existing indications for its use and discuss the relative outcomes...
March 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/27873029/induction-of-pluripotency-in-long-term-cryopreserved-human-neonatal-fibroblasts-in-feeder-free-condition
#18
Maria Csobonyeiova, Lubica Krajciova, Andreas Nicodemou, Stefan Polak, Lubos Danisovic
A novel approach for stem cell generation is the attempt to induce conversion of the adult somatic cells into pluripotent stem cells so called induced pluripotent stem cells (iPSCs) by introducing specific transcription factors. iPSCs have two essential cell characteristics, they are pluripotent and posses long term cell-renewal capacity. Additionally, iPSCs can be derived from patient-specific somatic cells, thus bypassing ethical and immunological issues. The aim of our study was to reprogram long-term cryopreserved human neonatal fibroblasts by new method using lipid nano-particle technology (Lipofectamine 3000 reagent transfection system) in combination with Epi 5 reprogramming vectors...
March 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/27838813/measurements-of-donor-endothelial-keratoplasty-lenticules-prepared-from-fresh-donated-whole-eyes-by-using-ultrasound-and-optical-coherence-tomography
#19
Mozhgan Rezaei Kanavi, Farzan Nemati, Tahereh Chamani, Bahar Kheiri, Mohammad Ali Javadi
This study was conducted to analyze the profile and thickness of endothelial keratoplasty lenticules prepared from fresh donated whole eyes with Visante optical coherence tomography (V-OCT) compared to measurements obtained from ultrasound pachymetry (USP) at the Central Eye Bank of Iran. Microkeratome-assisted precut corneas were prepared for Descemet stripping automated endothelial keratoplasty by using standard eye bank protocol. Central posterior lenticule thickness (CPLT) on fresh whole eye, before excising corneoscleral disc and transferring to Optisol-GS, was measured by USP...
March 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/27837287/status-quo-of-management-of-the-human-tissue-banks-in-taiwan
#20
REVIEW
Ching-Pang Chou, Szu-Cheng Chou, Ying-Hua Chen, Yu-Hsuan Chen, Ming-Shin Lee
As the technologies associated with transplantation and biological tissue engineering continue to advance, human cells and tissues form an integral part to the practice of regenerative medicine. The patient's use of tissues entails the risk of introducing, transmitting and spreading communicable diseases. To prevent such risk and to ensure that the human organs, tissues and cells remain intact and functional after being handled and processed, the transplanted tissues must be subject to good management standards through all stages of collection, screening, processing, storage and distribution as the safety of the users is of the utmost importance...
March 2017: Cell and Tissue Banking
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