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Cell and Tissue Banking

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https://www.readbyqxmd.com/read/28238108/enhanced-vascular-biocompatibility-of-decellularized-xeno-allogeneic-matrices-in-a-rodent-model
#1
M van Steenberghe, T Schubert, Y Guiot, C Bouzin, X Bollen, P Gianello
Glutaraldehyde preservation is the gold standard for cardiovascular biological prosthesis. However, secondary calcifications and the absence of tissue growth remain major limitations. Our study assessed in vitro and in vivo the biocompatibility of human (fascia lata, pericardium) and porcine tissues (pericardium, peritoneum) treated with a physicochemical procedure for decellularization and non-conventional pathogens inactivation. Biopsies were performed before and after treatment to assess decellularization (HE/Dapi staining/DNA quantification/MHC I/alpha gal immunostaining) and mechanical integrity...
February 25, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28236164/evaluation-of-the-effects-of-the-combination-of-autologous-mesenchymal-stem-cells-and-platelet-rich-plasma-on-structural-bone-allograft-healing
#2
Chang-Goo Park, Min Wook Joo, Jinyoung Jeong, Yong-Koo Kang, Da-Reum Lee
In this study, we evaluated the efficacy of the combination of autologous mesenchymal stem cells (MSCs) and platelet-rich plasma (PRP) for enhancing structural allogenic bone graft healing in rabbits. For these experiments segmental bone defects (1.5-2 cm) were generated on femoral diaphysis of New Zealand white rabbits and reconstructed via structural allogenic bone grafting and additional intramedullary nail fixation. The structural allografts were subsequently wrapped with Gelfoam containing autologous MSCs and PRP, or PRP alone (control)...
February 24, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28233169/can-stem-cells-enhance-bone-formation-in-the-human-edentulous-alveolar-ridge-a-systematic-review-and-meta-analysis
#3
REVIEW
Lucyene Miguita, Andrea Mantesso, Claudio Mendes Pannuti, Maria Cristina Zindel Deboni
Several non-biological materials are currently being used to increase the alveolar bone volume to support dental implants. Recently, stem cell therapy has emerged as a promising biological substitute or adjuvant to enhance bone healing. In order to determine if stem cell therapy has enough clinical evidence to bone ridge augmentation in humans, a systematic review and meta-analysis were conducted. Two independent investigators searched the Entrez PubMed, SCOPUS and Web of Science databases for eligible randomized clinical trials that describe stem cell therapies for alveolar bone formation...
February 23, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28138805/umbilical-cord-derived-mesenchymal-stem-cell-transplantation-for-treating-elderly-vascular-dementia
#4
Yan He, Xingfang Jin, Jun Wang, Mingyao Meng, Zongliu Hou, Weimeng Tian, You Li, Wenju Wang, Yunhong Wei, Yipeng Wang, Hongqiong Meng, Xuyang Lu, Zhuo Chen, Linyao Fu
This study aimed to determine the efficacy and safety of human umbilical cord-derived mesenchymal stem cell (HUC-MSC) transplantation for treating elderly vascular dementia (VaD). Ten VaD patients (average age, 73.88 years old) were treated. HUC-MSCs were isolated, cultured, stem cell-marked, and qualified and administered as a 3-course intravenous infusion to these patients. The Mini-Mental State Exam (MMSE) and the Activities of Daily Living Index (Barthel Index scoring system) were used to assess the cognitive function and daily living activity improvements in these patients before transplantation (T0), 3 months after transplantation (T1), and 6 months after transplantation (T2)...
January 30, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28116630/corneal-transplantation-study-of-the-data-of-a-regional-eye-bank-for-the-year-2013-and-analysis-of-the-evolution-of-the-adverse-events-reported-in-france-since-2010
#5
Anne-Sophie Gauthier, Marie Castelbou, Mélanie Bidaut Garnier, Joëlle Pizzuto, Stephan Roux, Philippe Gain, Fabienne Pouthier, Bernard Delbosc
To analyze the data of the adverse events collected in a single major eye bank (EFS Bourgogne Franche Comté, Besançon, France) for the year 2013 and to report the French data of biovigilance provided by the French National Agency for Medicines and Health Products Safety (ANSM) between 2010 and 2013. we have set up a study of adverse events in 2013, in collaboration with a single eye bank (EFS Bourgogne Franche Comté, Besançon, France). A survey was sent to the surgeon for each delivered corneal button by the eye bank in 2013...
January 23, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28058525/the-visual-assessment-of-broth-cultures-for-tissue-bank-samples
#6
Kerry Varettas
The bioburden screening process of allograft musculoskeletal tissue samples received at the South Eastern Area Laboratory Services includes the routine use of solid agar and cooked meat (CM) broth media. CM has been routinely sub-cultured onto solid agar plates after aerobic incubation at 35 °C. This study will evaluate whether a visual assessment of CM can replace sub-culture by an in vitro inoculation and a prospective study. Eight challenge organisms were serially diluted and inoculated into CM. The average inoculum of 0...
January 5, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28058524/determination-of-residual-dimethylsulfoxide-in-cryopreserved-cardiovascular-allografts
#7
R Díaz Rodríguez, B Van Hoeck, S De Gelas, F Blancke, R Ngakam, K Bogaerts, R Jashari
Dimethylsulfoxide (DMSO) is a solvent which protects the structure of allografts during the cryopreservation and thawing process. However, several toxic effects of DMSO in patients after transplantation of cryopreserved allografts have been described. The aim of this study is to determine the residual DMSO in the cardiovascular allografts after thawing and preparation of cryopreserved allografts for clinical application following guidelines of the European Pharmacopoeia for DMSO detection. Four types of EHB allografts (aortic valve-AV, pulmonary valve-PV, descending thoracic aorta-DA, and femoral artery-FA) are cryopreserved using as cryoprotecting solution a 10% of DMSO in medium 199...
January 5, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28004288/in-vivo-quantification-of-the-revascularization-of-a-human-acellular-dermis-seeded-with-epcs-and-mscs-in-co-culture-with-fibroblasts-and-pericytes-in-the-dorsal-chamber-model-in-pre-irradiated-tissue
#8
M Vitacolonna, D Belharazem, P Hohenberger, E D Roessner
INTRODUCTION: Transplantation of a cell-seeded graft may improve wound healing after radiotherapy. However, the survival of the seeded cells depends on a rapid vascularization of the graft. Co-culturing of adult stem cells may be a promising strategy to accelerate the vessel formation inside the graft. Thus, we compared the in vivo angiogenic potency of mesenchymal stem cells (MSC) and endothelial progenitor cells (EPC) using dorsal skinfold chambers and intravital microscopy. MATERIALS AND METHODS: Cells were isolated from rat bone marrow and adipose tissue and characterized by immunostaining and flow cytometry...
December 21, 2016: Cell and Tissue Banking
https://www.readbyqxmd.com/read/27999996/fresh-and-in-vitro-osteodifferentiated-human-amniotic-membrane-alone-or-associated-with-an-additional-scaffold-does-not-induce-ectopic-bone-formation-in-balb-c-mice
#9
Romain Laurent, Aurélie Nallet, Benoit de Billy, Laurent Obert, Laurence Nicod, Christophe Meyer, Pierre Layrolle, Narcisse Zwetyenga, Florelle Gindraux
The human amniotic membrane (hAM) has been successfully used as a natural carrier containing amniotic mesenchymal stromal cells, epithelial cells and growth factors. It has a little or no immunogenicity, and possesses useful anti-microbial, anti-inflammatory, anti-fibrotic and analgesic properties. It has been used for many years in several indications for soft tissue repair. We previously reported that hAM represents a natural and preformed sheet containing highly potent stem cells, and could thus be used for bone repair...
December 20, 2016: Cell and Tissue Banking
https://www.readbyqxmd.com/read/27928705/semi-quantitative-assessments-of-dextran-toxicity-on-corneal-endothelium-conceptual-design-of-a-predictive-algorithm
#10
Filip Filev, Ceprail Oezcan, Jana Feuerstacke, Stephan J Linke, Birgit Wulff, Olaf J C Hellwinkel
Dextran is added to corneal culture medium for at least 8 h prior to transplantation to ensure that the cornea is osmotically dehydrated. It is presumed that dextran has a certain toxic effect on corneal endothelium but the degree and the kinetics of this effect have not been quantified so far. We consider that such data regarding the toxicity of dextran on the corneal endothelium could have an impact on scheduling and logistics of corneal preparation in eye banking. In retrospective statistic analyses, we compared the progress of corneal endothelium (endothelium cell loss per day) of 1334 organ-cultured corneal explants in media with and without dextran...
December 7, 2016: Cell and Tissue Banking
https://www.readbyqxmd.com/read/27761677/tissue-recovery-practices-and-bioburden-a-systematic-review
#11
REVIEW
S Brubaker, K Lotherington, Jie Zhao, B Hamilton, G Rockl, A Duong, A Garibaldi, N Simunovic, D Alsop, D Dao, R Bessemer, O R Ayeni
For successful transplantation, allografts should be free of microorganisms that may cause harm to the allograft recipient. Before or during recovery and subsequent processing, tissues can become contaminated. Effective tissue recovery methods, such as minimizing recovery times (<24 h after death) and the number of experienced personnel performing recovery, are examples of factors that can affect the rate of tissue contamination at recovery. Additional factors, such as minimizing the time after asystole to recovery and the total time it takes to perform recovery, the type of recovery site, the efficacy of the skin prep performed immediately prior to recovery of tissue, and certain technical recovery procedures may also result in control of the rate of contamination...
December 2016: Cell and Tissue Banking
https://www.readbyqxmd.com/read/27757728/reduced-rates-of-non-union-with-modified-periacetabular-osteotomy-using-peracetic-acid-sterilized-cancellous-allografts
#12
Georgi I Wassilew, Viktor Janz, Lisa Renner, Carsten Perka, Axel Pruss
The objective of the present study was to analyze the clinical and radiological results of periacetabular osteotomies (PAO) using Kirschner wire fixation and an allogeneic cancellous bone graft. This retrospective cohort study included 73 patients (85 PAOs). The allografts were processed from distal femur of cadaveric donors, defatted, sterilized with a peracetic-acid ethanol solution and freeze-dried. The clinical outcome, as measured by the Harris Hip Scores (HHS), the complication rate and the acetabular correction, as measured by radiological parameters, were compared...
December 2016: Cell and Tissue Banking
https://www.readbyqxmd.com/read/27757727/analysis-of-the-effectiveness-of-sodium-hypochlorite-decontamination-of-cadaveric-human-tissues-at-retrieval
#13
Adolfo Paolin, Diletta Trojan, Antonio Carniato, Fabio Tasca, Ervino Massarin, Alessandro Tugnoli, Elisa Cogliati
Bacterial contamination of tissues retrieved from cadaveric donors is a common feature worldwide, and every tissue bank, albeit using different methods, conducts decontamination to guarantee safe tissues suitable for clinical use. The effectiveness of the methods used to eradicate pathogens differs. In order to reduce the tissue bioburden at retrieval, we have introduced a new method involving rinsing tissues in a sodium hypochlorite solution. To test its effectiveness we analyzed two comparable groups of tissues: Group A: 1881 tissues, all rinsed with isotonic saline solution after retrieval, and Group B: 1968 tissues immersed in an isotonic saline solution containing sodium hypochlorite (final concentration 0...
December 2016: Cell and Tissue Banking
https://www.readbyqxmd.com/read/27718130/tissue-reaction-of-deproteinized-bovine-bone-matrix-grafting-in-ectopic-site-histological-study-on-sheep
#14
João Ricardo Almeida Grossi, Rodrigo Bonacin, Viviane Rozeira Crivelaro, Allan Fernando Giovanini, João César Zielak, Tatiana Miranda Deliberador
The aim of this paper was to evaluate through histological analysis of the tissue reaction of deproteinized bovine bone matrix (DBBM) when inserted into the site of intramuscular ectopic sheep. In this study, 16 sheep received 3 groups and these were divided into 2 experimental times: Group 1-sham group, Group 2-particulate autogenous bone and Group 3-DBBM granules. All animals underwent surgical procedures for insertion of materials in an ectopic site (muscles of the lower back and after 3 and 6 months postoperatively, the samples were evaluated by histological analysis...
December 2016: Cell and Tissue Banking
https://www.readbyqxmd.com/read/27677903/cytotoxicity-testing-of-a-polyurethane-nanofiber-membrane-modified-with-chitosan-%C3%AE-cyclodextrin-berberine-suitable-for-wound-dressing-application-evaluation-of-biocompatibility
#15
Monika Klempaiová, Jana Dragúňová, Peter Kabát, Mária Hnátová, Ján Koller, Dušan Bakoš
In this study we evaluated the biocompatibility of a modified polyurethane nanofiber membrane on a polypropylene spunbond substrate. This material was treated with plasma using diffuse coplanar surface barrier discharge, and subsequent modification was done by continuous spraying of a biologically active chitosan solution (CHIT) containing an inclusion complex of β-cyclodextrin (β-CD) encapsulating berberine (BRB). Biocompatibility was evaluated using several in vitro assays. Human dermal fibroblasts (HDFs) and 3T3 murine fibroblasts were used as biological models...
December 2016: Cell and Tissue Banking
https://www.readbyqxmd.com/read/27677902/endemic-impact-of-human-t-cell-leukemia-virus-type-1-screening-in-bone-allografts
#16
Yasuhiro Ishidou, Kanehiro Matsuyama, Eiji Matsuura, Takao Setoguchi, Satoshi Nagano, Hironori Kakoi, Masataka Hirotsu, Ichiro Kawamura, Takuya Yamamoto, Setsuro Komiya
Allograft bone is a widely used as a convenient tool for reconstructing massive bone defects in orthopedic surgery. However, allografts are associated with the risk of viral disease transmission. One of the viruses transmitted in this manner is human T-lymphotropic virus type 1 (HTLV-1), which is found worldwide but is unevenly distributed. The southwestern parts of Japan are a highly endemic for HTLV-1. We investigated the HTLV-1 seroprevalence in candidate allograft donors at the regional bone bank in Kagoshima, Japan during its first 5 years of service...
December 2016: Cell and Tissue Banking
https://www.readbyqxmd.com/read/27665294/disinfection-of-human-musculoskeletal-allografts-in-tissue-banking-a-systematic-review
#17
REVIEW
J Mohr, M Germain, M Winters, S Fraser, A Duong, A Garibaldi, N Simunovic, D Alsop, D Dao, R Bessemer, O R Ayeni
Musculoskeletal allografts are typically disinfected using antibiotics, irradiation or chemical methods but protocols vary significantly between tissue banks. It is likely that different disinfection protocols will not have the same level of microorganism kill; they may also have varying effects on the structural integrity of the tissue, which could lead to significant differences in terms of clinical outcome in recipients. Ideally, a disinfection protocol should achieve the greatest bioburden reduction with the lowest possible impact on tissue integrity...
December 2016: Cell and Tissue Banking
https://www.readbyqxmd.com/read/27631324/allograft-selection-for-distal-femur-through-cutting-contour-registration
#18
Lei Qiu, Yu Zhang, Qing Zhang, Lihui Xu, Xiaohui Niu, Li Zhang
Allograft reconstruction is an acceptable procedure for the recovery of normal anatomy after the bone tumor resection. During the past few years, several automated methods have been proposed to select the best anatomically matching allograft from the virtual donor bone bank. The surface-based automated method uses the contralateral healthy bone to obtain the normal surface shape of the diseased bone, which could achieve good matching of the defect and the selected allograft. However, the surface-based method focuses on the matching of the whole bone so that the matching of the contact surface between the allograft and the recipient bone may not be optimal...
December 2016: Cell and Tissue Banking
https://www.readbyqxmd.com/read/27631323/lyophilized-allogeneic-bone-tissue-as-an-antibiotic-carrier
#19
Débora C Coraça-Huber, Christoph G Ammann, Michael Nogler, Manfred Fille, Lars Frommelt, Klaus-Dieter Kühn, Christian Fölsch
The rising number of primary joint replacements worldwide causes an increase of revision surgery of endoprostheses due bacterial infection. Revision surgery using non-cemented implants seems beneficial for the long-term outcome and the use of antibiotic-impregnated bone grafts might control the infection and give a good support for the implant. In this study we evaluated the release of antibiotics from fresh-frozen and lyophilized allogeneic bone grafts. Lyophilized bone chips and fresh frozen bone chips were mixed with gentamicin sulphate, gentamicin palmitate, vancomycin, calcium carbonate/calcium sulphate impregnated with gentamicin sulphate, and calcium carbonate/calcium sulphate bone substitute material impregnated with vancomycin...
December 2016: Cell and Tissue Banking
https://www.readbyqxmd.com/read/27604467/evaluation-of-new-antibiotic-cocktails-against-contaminating-bacteria-found-in-allograft-tissues
#20
Agnese Serafini, Erika Riello, Diletta Trojan, Elisa Cogliati, Giorgio Palù, Riccardo Manganelli, Adolfo Paolin
Contamination of retrieved tissues is a major problem for allograft safety. Consequently, tissue banks have implemented decontamination protocols to eliminate microorganisms from tissues. Despite the widespread adoption of these protocols, few comprehensive studies validating such methods have been published. In this manuscript we compare the bactericidal activity of different antibiotic cocktails at different temperatures against a panel of bacterial species frequently isolated in allograft tissues collected at the Treviso Tissue Bank Foundation, a reference organization of the Veneto Region in Italy that was instituted to select, recover, process, store and distribute human tissues...
December 2016: Cell and Tissue Banking
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