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Cell and Tissue Banking

Daniel Trujillo Diaz, Nora C Hernandez, Etty P Cortes, Phyllis L Faust, Jean Paul G Vonsattel, Elan D Louis
A review of the brain banking literature reveals a primary focus either on the factors that influence the decision to become a future donor or on the brain tissue processing that takes place after the individual has died (i.e., the front-end or back-end processes). What has not been sufficiently detailed, however, is the complex and involved process that takes place after this decision to become a future donor is made yet before post-mortem processing occurs (i.e., the large middle-ground). This generally represents a period of many years during which the brain bank is actively engaged with donors to ensure that valuable clinical information is prospectively collected and that their donation is eventually completed...
September 15, 2018: Cell and Tissue Banking
Giulia Montagner, Diletta Trojan, Elisa Cogliati, Flavio Manea, Andrea Vantini, Adolfo Paolin
Although careful donor selection reduces tissue contamination, close microbiological control of harvested allografts remains a key task of tissue banks. To guarantee the safety of human tissues for allograft transplantation, a decontamination regimen must be adopted which, as recommended by European guidelines, is active against the majority of microorganisms isolated in tissues. Antibiotic decontamination methods differ from one tissue bank to another in terms of antimicrobial agents, temperature and length of exposure...
September 11, 2018: Cell and Tissue Banking
M J Eagle, J Man, P Rooney, J N Kearney
NHS Blood and Transplant Tissue and Eye Services banks and issues, cut, shaped and washed bone from deceased donors. The bone is cut/shaped prior to washing and then processed to remove up to 99.9% of blood, bone marrow and associated cells. The processed bone is then sterilised by gamma irradiation with or without a freeze-drying step. Removal of donor blood and bone marrow has been reported to aid incorporation of allograft bone without affecting the biomechanical properties of the bone. However, cut and shaped bone is not suitable for some orthopaedic procedures and some orthopaedic surgeons do not wish to use irradiated bone...
September 11, 2018: Cell and Tissue Banking
Abhinav Golla, Li Wang, Caleb Morris, Mitchell P Weikert, Sumitra S Khandelwal
To evaluate the repeatability of central corneal thickness (CCT) measurements in donor corneas using optical coherence tomography (OCT, RTVue-Optovue, Inc., Fremont, CA). Consecutive corneas were measured by a single observer using the RTVue. All corneas were preserved in the Transend chamber and Life4 °C media (Numedis, Inc., Isanti, MN/USA) and stored at 4 °C. The repeatability was evaluated using a pooled within-subject standard deviation (SD), coefficient of variation (CoV), and intraclass correlation coefficient (ICC)...
September 4, 2018: Cell and Tissue Banking
Zhiguo He, Julie Gueudry, David Toubeau, Philippe Gain, Gilles Thuret, Marc Muraine
Our aim was to measure the endothelial quality of prestripped Descemet membrane endothelial keratoplasty (DMEK) 48 h after preparation in an eye bank with the Muraine technique and shipping in a distant center. Ten pairs of human corneas with similar eye bank endothelial cell density (ebECD) were stored in organ-culture (OC) for 25 days (20, 28) [median (10-90 percentiles)]. One cornea was then randomized to DMEK preparation using the Moria Muraine trephine, the other served as control. The grafts were left attached to the center of the cornea, immersed in the OC medium (without Dextran) and shipped to a distant center...
August 31, 2018: Cell and Tissue Banking
Noëmi M C De Roo, Laurent A M Thierens, Liesbeth Temmerman, Elise Dekeyser, Jerina Boelens, Guy A M De Pauw
Autotransplantation is a versatile technique for the replacement of a missing tooth and cryopreservation can expand its scope. The aim of this in vitro study is to compare the antimicrobial effect of different transport protocols on procured teeth prior to cryopreservation. Streptococcus oralis biofilms were grown on ten sterile premolars, incubated for 48 h and subjected to the following transport procedures: an untreated (contaminated) control group, a group rinsed with phosphate buffered saline (PBS), a group transported in PBS, a group transported in Dulbecco's Modified Eagle's medium (DMEM) supplemented with fetal calf serum (FCS), and a group transported in DMEM supplemented with FCS and antibiotics (AB)...
August 29, 2018: Cell and Tissue Banking
Elizaveta Padalko, Katrien Lagrou, Marie-Luce Delforge, Hilde Jansens, Nadine Ectors, Jean-Paul Pirnay, Johan Klykens, Etienne Sokal, Ludo Muylle, Agnes Libois, Alain Vanderkelen, Gilbert Verbeken, Conny Matthys, Dominique Goossens, Geert Hanssens, Muriel Baltes, Hilde Beele
This paper on the biological tests carried out on serum/plasma samples from donors of human body material (HBM) is the result of a project of the working Group of Superior Health Council of Belgium formed with experts in the field of HBM and infectious serology. Indeed, uncertainty about the interpretation of biological test results currently leads to the sometimes unjustified cancelling of planned donations or the rejection of harvested HBM, whilst more sophisticated diagnostic algorithms would still allow the use of organs or HBM that would otherwise have been rejected...
August 29, 2018: Cell and Tissue Banking
Luz María Ruíz-Godoy, Fabiola Berenice Baez-Revueltas, María de Lourdes Suarez-Roa, Héctor Maldonado-Martínez, Virginia Enriquez-Carcamo, Ana Laura Colín-González, Abelardo Meneses-García
In this work we estimated the budgetary impact of the samples produced by the biobank of the "Instituto Nacional de Cancerología" (BT-INCan) to set a recuperation fee from the perspective of the Health Ministry of Mexico. The study is an observational retrospective review of the direct medical costs (DMCs) of the processes involved in cryopreservation of the samples collected, on a per sample basis, including materials, laboratory tests, personnel, and administrative costs. Materials and labor costs were determined by information collected from the BT-INCan...
August 28, 2018: Cell and Tissue Banking
Halimeh Hassanzadeh, Maryam M Matin, Hojjat Naderi-Meshkin, Hamid Reza Bidkhori, Mahdi Mirahmadi, Mahmood Raeesolmohaddeseen, Nasser Sanjar-Moussavi, Ahmad Reza Bahrami
Recent advances in wound healing have made cell therapy a potential approach for the treatment of various types of skin defects such as trauma, burns, scars and diabetic leg ulcers. Cultured keratinocytes have been applied to burn patients since 1981. Patients with acute and chronic wounds can be treated with autologous/allograft cultured keratinocytes. There are various methods for cultivation of epidermal keratinocytes used in cell therapy. One of the important properties of an efficient cell therapy is the preservation of epidermal stem cells...
August 13, 2018: Cell and Tissue Banking
Senem Maral, Murat Albayrak, Cigdem Pala, Abdulkerim Yildiz, Osman Sahin, Hacer Berna Ozturk
Various side effects associated with dimethyl sulfoxide (DMSO) which is used for cryopreservation of bone marrow or peripheral blood progenitor cells (PBPCs) have been reported. Among the central nervous system side effects the epileptic seizures, stroke, transient and temporary leucoencephalopathy, and global amnesia are well known. Herein we report a 52-year-old man who experienced tonic-clonic seizure within minutes after the initiation of DMSO cryopreserved autologous PBPC infusion. Unfortunately, he also developed cardiac arrest and required intubation for ventilation after the seizure...
August 11, 2018: Cell and Tissue Banking
Guilherme G Naves, André F Silva, Uri Antebi, Priscila C Cristovam, Emerson K Honda, Rodrigo P Guimarães
Tissue Banks have become the main source for bone grafts, due to preference for homologous tissues. Notwithstanding the use of aseptic techniques for procurement of tissues and judicious selection of donors, microorganisms are frequently found in procured bones. Purpose of this study is to evaluate the factors that increase safety of procurement and minimize discard of procured tissues. Microbiological contamination was analyzed in 1271 musculoskeletal tissues removed from 138 multi-organ donors over a period extending from 2006 to 2016...
August 1, 2018: Cell and Tissue Banking
Ying Tian, Haifeng Zhu, Jie Wu, Shuangyong Wang
To observe the effect of DMEM/F12 pegylated with methoxy polyethylene glycol succinimidyl propionate (mPEG-SPA) on the biophysical and immune characteristics of the rat cornea preserved in it. Corneal grafts were harvested from Wistar rat and preserved in the DMEM/F12 plus mPEG-SPA, DMEM/F12 without mPEG-SPA, and standard Optisol-GS solution at 4 °C for 14 days, referred as plus-PEG, minus-PEG, and Optisol grafts, respectively. The biophysical properties of those grafts, including transmittance, thickness, water content, and biomechanics were investigated...
August 1, 2018: Cell and Tissue Banking
Young Hwan Choi, Young Soon Cho, Jong Ho Lee, Yanghwan Choi, Soo Young Noh, Seungchoon Park, Changmin Sung, Jin Kyu Lim, Jongdae Kim, Jae Jun Shin, Banseok Yang, Jiyun Jeong, Huan Chun, Kwang Jo Kim
Cadaver skin is used for temporary wound covering, but there is insufficient evidence regarding its clinical usefulness in patients with major burns. We aimed to analyze the effect of cadaveric skin allograft on mortality rates in patients with burns involving > 30% of total body surface area (TBSA). Our study included 1282 patients with > 30% of TBSA burned admitted to four hospitals in Korea between June 1, 2008 and December 31, 2016. Of these, 698 patients underwent cadaver skin allograft (cadaver group), and 584 were treated with conventional treatment (non-cadaver group)...
July 31, 2018: Cell and Tissue Banking
Ingrida Smeringaiova, Otakar Nyc, Peter Trosan, Jaroslav Spatenka, Jan Burkert, Jan Bednar, Katerina Jirsova
Two decontamination solutions, commercially produced BASE•128 and laboratory decontamination solution (LDS), with analogous content of antibiotic and antimycotic agents, were compared in their antimicrobial efficiency and stability (pH and osmolarity). Both solutions were compared immediately after thawing aliquots frozen for 1, 3 or 6 months. Agar well diffusion method was used to test their antimicrobial efficiency against five human pathogens: Staphylococcus aureus, Pseudomonas aeruginosa, Proteus mirabilis, Escherichia coli and Enterococcus faecalis...
July 30, 2018: Cell and Tissue Banking
Peng Chen, Changqing Jiang, Li Shen, Wentao Zhang, Lixin Zhu
The aim of this study was to explore the biological effects of the amount of chemical extraction treatments performed on an allogeneic tendon through histomorphology, biological mechanics testing, and an immunogenicity assay. Sixteen New Zealand rabbits (body weight 2.5-3.0 kg) were randomly divided into four groups: group A (chemical extraction once), group B (chemical extraction twice), group C (chemical extraction three times), and group D (blank control group), with four rabbits in each group. The Achilles tendons of each rabbit were separated and subjected to a chemical extraction process with Triton X-100 and sodium deoxycholate, followed by hematoxylin and eosin staining, electron microscopy observation, biomechanical testing, and mixed lymphocyte culture...
July 30, 2018: Cell and Tissue Banking
S Mohd, M I Ghazali, N Yusof, S Sulaiman, S Ramalingam, T Kamarul, A Mansor
Air-dried and sterilized amnion has been widely used as a dressing to treat burn and partial thickness wounds. Sterilisation at the standard dose of 25 kGy was reported to cause changes in the morphological structure as observed under the scanning electron microscope. This study aimed to quantify the changes in the ultrastructure of the air-dried amnion after gamma-irradiated at several doses by using atomic force microscope. Human placentae were retrieved from mothers who had undergone cesarean elective surgery...
July 28, 2018: Cell and Tissue Banking
Apostolos Lazaridis, Dimitrios Brouzas, Walter Sekundo, Ilias Georgalas, George Kymionis, Klio Chatzistefanou, Chryssanthi Koutsandrea, Konstantinos Droutsas
To evaluate the efficacy and outcomes of tectonic epikeratoplasty with use of ethanol-preserved corneal grafts for the management of perforated corneal melts. The present retrospective case series includes 10 eyes which underwent tectonic epikeratoplasty for perforated corneal melts. The stromal remainders of Descemet membrane endothelial keratoplasty (DMEK) and Descemet stripping automated endothelial keratoplasty (DSAEK) graft preparation were stored in 95% ethanol and used as emergency tectonic grafts for restoring globe integrity after sterile and infectious perforated corneal melts...
July 28, 2018: Cell and Tissue Banking
Gholam Reza Dashti, Zohre Nateghian, Farhad Golshan Iranpour
Sperm motility is the result of transverse movements that exist along its tail. It plays an important role in male fertility. The aim of this study was to evaluate the influence of keeping washed normozoospermic semen samples at 4-6 and 25 °C on the motility of spermatozoa. 26 semen samples of normozoospermic were washed twice in modified Ham's F10 medium. Then, thirteen of the semen samples were kept in refrigerator (4-6 °C) and the remaining samples were stored in incubator (25 °C) for 12 days. On the 0 (immediately after sampling as control group), 1st, 2nd, 5th, 7th and the 12th days, the percentage of fast progressive (grade a), slow progressive (grade b), non-progressive (grade c) and immotile (grade d) sperm cells were calculated for each temperature...
July 27, 2018: Cell and Tissue Banking
Mozhgan Rezaei Kanavi, Tahereh Chamani, Bahareh Kheiri, Mohammad Ali Javadi
This study was conducted to analyze the preoperative thickness profile and endothelial rating of ultrathin Descemet's stripping automated endothelial keratoplasty (UT-DSAEK) tissues prepared with a single versus double microkeratome pass from donated whole eyes and corresponding eye bank postoperative results. Microkeratome-assisted UT-DSAEK tissues were prepared from freshly donated whole eyes with single-pass (SP) and double-pass (DP) technique in the Central Eye Bank of Iran. Preoperative thickness profiles and endothelial cell densities of UT-DSAEK tissues were obtained from optical coherence tomography and specular microscopy, respectively, and compared between groups...
July 14, 2018: Cell and Tissue Banking
Mazyar Irani, Vedran Lovric, William R Walsh
Tendon allografts, when autograft options are limited or when obtaining an autograft is not aligned with the patients' best interest, play an important role in tendon and ligament reconstruction. To minimize the risk of infectious disease transmission tissue banks perform screening tests and the allografts cleaned are sterilized. The current study examines and compares the initial mechanical properties and histological appearance of supercritical CO2 (SCCO2 )-treated and gamma-irradiated porcine extensor tendons...
July 13, 2018: Cell and Tissue Banking
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