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Cell and Tissue Banking

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https://www.readbyqxmd.com/read/29330637/assessment-of-the-permeability-properties-of-cryopreservation-outer-bags-used-in-nhsbt
#1
P Hogg, P Rooney, R Lomas, J N Kearney
This study was carried out to investigate leakage/transport across the bag material of six outer cryopreservation bags in common use within NHS Blood and Transplant. In order to do this two different leak testing procedures; coloured dye and hydrogen tracer gas, were used. The data obtained show that a coloured dye cannot permeate through the materials both at room temperature and following storage at liquid nitrogen temperature (- 196 °C). In addition, when filled with the smallest elemental molecule, hydrogen, in the form of a tracer gas, all of the bags only allowed trace amounts of hydrogen to escape, either through the seal or the bag material...
January 12, 2018: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29313189/supportive-properties-of-basement-membrane-layer-of-human-amniotic-membrane-enable-development-of-tissue-engineering-applications
#2
Sonia Iranpour, Nasser Mahdavi-Shahri, Raheleh Miri, Halimeh Hasanzadeh, Hamid Reza Bidkhori, Hojjat Naderi-Meshkin, Ehsan Zahabi, Maryam M Matin
Human amniotic membrane (HAM) has been widely used as a natural scaffold in tissue engineering due to many of its unique biological properties such as providing growth factors, cytokines and tissue inhibitors of metalloproteinases. This study aimed at finding the most suitable and supportive layer of HAM as a delivery system for autologous or allogeneic cell transplantation. Three different layers of HAM were examined including basement membrane, epithelial and stromal layers. In order to prepare the basement membrane, de-epithelialization was performed using 0...
January 8, 2018: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29270824/correction-to-comparison-of-the-effect-of-activated-or-non-activated-prp-in-various-concentrations-on-osteoblast-and-fibroblast-cell-line-proliferation
#3
Surena Vahabi, Zahra Yadegari, Hossein Mohammad-Rahimi
In the original publication of this article, the affiliation of the corresponding author has been published incorrectly. Now the correct affiliation has been provided in this erratum.
December 21, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29264694/successful-disinfection-of-femoral-head-bone-graft-using-high-hydrostatic-pressure
#4
Michiel A J van de Sande, Judith V M G Bovée, Mark van Domselaar, Marja J van Wijk, Ingrid Sanders, Ed Kuijper
The current standard for sterilization of potentially infected bone graft by gamma irradiation and thermal or chemical inactivation potentially deteriorates the biomechanical properties of the graft. We performed an in vitro experiment to evaluate the use of high hydrostatic pressure (HHP); which is widely used as a disinfection process in the food processing industry, to sterilize bone grafts. Four femoral heads were divided into five parts each, of which 16 were contaminated (in duplicate) with 105-107 CFU/ml of Staphylococcus epidermidis, Bacillus cereus, or Pseudomonas aeruginosa or Candida albicans, respectively...
December 20, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29264693/the-effects-of-gelatin-fibrin-platelet-glue-and-their-combination-on-healing-of-the-experimental-critical-bone-defect-in-a-rat-model-radiological-histological-scanning-ultrastructural-and-biomechanical-evaluation
#5
Hojjat Gholipour, Abdolhamid Meimandi-Parizi, Ahmad Oryan, Amin Bigham Sadegh
Fibrin-platelet glue (FPG) is a blood derivative, in which platelets and fibrinogen are concentrated in a small plasma volume, by differential centrifugation and precipitation. It can form a three-dimensional and biocompatible fibrin scaffold with a myriad of growth factors and proteins that are released progressively to the local environment and contribute to the accelerated postoperative bone healing. Gelatin (Gel) is a derivative of collagen and can promote cell adhesion and proliferation due to its unique sequence of amino acids, so it is suitable for bone tissue applications...
December 20, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29230596/distribution-of-potential-eye-and-tissue-donors-within-an-australian-teaching-hospital
#6
Martin J Dutch, Anthony F Denahy
Eye and Tissue donation has the capacity to transform lives, yet the vast majority of potential in-hospital donors are not recognised. Studies which describe the relative importance of specific units or wards in determining the size of the donor pool are limited. The aim of this study was to map the distribution of potential Eye and Tissue donors within the study hospital. A 12-month retrospective analysis of all patient deaths at the study hospital was undertaken. The ability to donate corneal, heart valve, bone and skin tissue was investigated...
December 11, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29222694/preparation-and-evaluation-of-decellularized-porcine-carotid-arteries-cross-linked-by-genipin-the-preliminary-results
#7
Yongquan Gu, Fei Wang, Rong Wang, Jianxin Li, Cong Wang, Liqiang Li, Zeqin Xu, Jian Zhang
Decellularized arteries have been considered as promising scaffolds for small-diameter vascular substitutes. However, weakened mechanical properties, immunological rejection and rapid degradation after transplantation still exist after decellularization. Previous studies indicated that genipin cross-linking can solve these problems. Therefore, genipin was selected as the cross-linking agent for the pre-treatment of decellularized arteries in our study. Histological analysis, scanning electron microscopy, mechanical properties analysis and subcutaneous embedding experiment were adopted to investigate the efficiency of decellularization and the effect of genipin cross-linking on improving mechanical, structural and biological properties of decellularized arteries...
December 8, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29218427/the-role-of-nonautologous-and-autologous-adipose-derived-mesenchymal-stem-cell-in-acute-pyelonephritis
#8
Shabnam Sabetkish, Nastaran Sabetkish, Mahba Ataei Talebi, Shahnaz Halimi, Abdol-Mohammad Kajbafzadeh
We compared the therapeutic effects of autologous and nonautologous adipose-derived mesenchymal stem cell (ADMSC), in ameliorating the renal function in a rabbit model of acute pyelonephritis. The difference of perirenal and neck subcutaneous ADMSCs were also evaluated. Twenty female rabbits were apportioned to 5 groups. In group I (n = 4), the rabbits were injected direct inoculation of Escherichia coli (E. coli) into the right kidney. In group II (n = 4), autologous ADMSCs obtained from nape adipose tissue were injected into the subcapsular space 1 week after E...
December 7, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29188402/development-and-characterisation-of-a-large-diameter-decellularised-vascular-allograft
#9
A Aldridge, A Desai, H Owston, L M Jennings, J Fisher, P Rooney, J N Kearney, E Ingham, S P Wilshaw
The aims of this study were to develop a biological large diameter vascular graft by decellularisation of native human aorta to remove the immunogenic cells whilst retaining the essential biomechanical, and biochemical properties for the ultimate benefit of patients with infected synthetic grafts. Donor aortas (n = 6) were subjected to an adaptation of a propriety decellularisation process to remove the cells and acellularity assessed by histological analysis and extraction and quantification of total DNA...
November 29, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29159500/comparative-analysis-of-molecular-activity-in-dermal-mesenchymal-stem-cells-from-different-passages
#10
Xinhua Li, Junqin Li, Xincheng Zhao, Qiang Wang, Xiaohong Yang, Yueai Cheng, Min Zhou, Gang Wang, Erle Dang, Xiaoli Yang, Ruixia Hou, Peng An, Guohua Yin, Kaiming Zhang
Mesenchymal stem cells (MSCs) are used for tissue regeneration in several pathological conditions, including autoimmune diseases. However, the optimal sources and culture requirements for these cells are still under investigation. Here, we compared mRNA expression in dermal MSCs (DMSCs) at passage (P) 3 and P5 to provide a reference for future studies related to DMSCs expansion. In normal DMSCs, the expression of three of eight genes associated with basic cellular activity were different at P5 compared to that at P3: PLCB4 and SYTL2 were upregulated by 4...
November 20, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29079991/establishment-of-a-porcine-corneal-endothelial-organ-culture-model-for-research-purposes
#11
Berenike C Kunzmann, Olaf J C Hellwinkel, Christian Klameth, Daniel Wenzel, Karl U Bartz-Schmidt, Martin S Spitzer, Maximilian Schultheiss
Human corneas usually are not available for research, as they are used for transplantation only. At the same time, scientific studies on cultured human endothelial cells can produce misleading results due to inevitable dedifferentiation. Therefore, an organ-culture model of porcine corneas-displaying endothelial cell death rates comparable to those of cultured human corneas-would be very desirable. Fresh pig eyes were prepared under sterile conditions to obtain corneoscleral buttons, corneal buttons and so called "split corneal buttons" (new preparation method) and cultivated for 15 days...
October 27, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29075945/use-of-freeze-dried-bone-allografts-in-osteoporotic-patients-undergoing-median-sternotomy
#12
Mei Liang, Jianzhou Liu, Qi Miao, Guotao Ma, Xingrong Liu, Xiaofeng Li, Chaoji Zhang
Osteoporosis is a major risk factor for deep sternal wound infection, which is a rare but serious complication after median sternotomy. We investigated the incidence of deep sternal wound infection and the protective effect of bone allografts in osteoporotic patients after sternal approximation. Data were collected retrospectively from consecutive osteoporotic patients who underwent cardiac surgery via median sternotomy. Sternal approximation in the historical control group was performed with conventional steel wire sutures...
October 26, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29071453/donor-cornea-tissue-in-cases-of-drowning-or-water-submersion-eye-banks-practice-patterns-and-tissue-outcomes
#13
Nithya P Vijayakumar, Purak Parikh, Shahzad I Mian, Brad Tennant, Gregory H Grossman, Bob Albrecht, Leslie M Niziol, Maria A Woodward
Surgical use of donor corneal tissue from victims of water submersion (drowning or submersion secondary to death) remains controversial due to limited evidence about the quality of these tissues. To assess the safety of donor corneal tissue from victims of water submersion, an investigation of eye banks' practice patterns and tissue outcomes was conducted. All 79 Eye Bank Association of America accredited eye banks were contacted for a phone interview of practices regarding tissue from victims of water submersion...
October 25, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29043523/analysis-of-potential-factors-affecting-allografts-contamination-at-retrieval
#14
A Paolin, C Romualdi, L Romagnoli, D Trojan
The microbiological contamination of retrieved tissues has become a very important topic and it is a critical aspect in the safety of allografts, especially from multi-tissue donors whose tissues are frequently contaminated as a consequence of retrieval. We analysed a total of 10,107 tissues, 8178 musculoskeletal and 1929 cardiovascular tissues, retrieved from 978 multi-tissue donors. Of these, 159 heart-beating donors (HBD) were also organ donors, while the remaining 819 non-heart-beating donors (NHBD) were tissue donors only...
December 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28799106/chemical-sterilization-of-allograft-dermal-tissues
#15
Abigail Phipps, Edward Vaynshteyn, John B Kowalski, Manh-Dan Ngo, Karen Merritt, Joel Osborne, Evangelia Chnari
Common terminal sterilization methods are known to alter the natural structure and properties of soft tissues. One approach to providing safe grafts with preserved biological properties is the combination of a validated chemical sterilization process followed by an aseptic packaging process. This combination of processes is an accepted method for production of sterile healthcare products as described in ANSI/AAMI ST67:2011. This article describes the validation of the peracetic acid and ethanol-based (PAAE) chemical sterilization process for allograft dermal tissues at the Musculoskeletal Transplant Foundation (MTF, Edison, NJ)...
December 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28717878/the-isolation-of-primary-hepatocytes-from-human-tissue-optimising-the-use-of-small-non-encapsulated-liver-resection-surplus
#16
Charlotte J Green, Catriona A Charlton, Lai-Mun Wang, Michael Silva, Karl J Morten, Leanne Hodson
Two-step perfusion is considered the gold standard method for isolating hepatocytes from human liver tissue. As perfusion may require a large tissue specimen, which is encapsulated and has accessible vessels for cannulation, only a limited number of tissue samples may be suitable. Therefore, the aim of this work was to develop an alternative method to isolate hepatocytes from non-encapsulated and small samples of human liver tissue. Healthy tissue from 44 human liver resections were graded for steatosis and tissue weights between 7...
December 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29043524/improving-the-success-rate-of-human-corneal-endothelial-cell-cultures-from-single-donor-corneas-with-stabilization-medium
#17
D Spinozzi, A Miron, M Bruinsma, J T Lie, I Dapena, S Oellerich, G R J Melles
Main objective of this study was to improve the success rate of human corneal endothelial cell (hCEC) cultures from single donor corneas. We could show that the use of stabilization medium prior to cell isolation may have a positive effect on the success rate of hCEC cultures from single research-grade donor corneas by allowing growth of otherwise possibly not successful cultures and by improving their proliferative rate. hCEC were obtained from corneo-scleral rims of 7 discarded human research-grade cornea pairs...
October 17, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29043522/cryovial-monolayer-vitrification-for-ovarian-tissue-cryopreservation
#18
Hongxia Li, Haixia Cao, Xingping Guo, Huaixiu Wang
The cryopreservation of ovarian tissue has been proved to be effective for fertility preservation. To find a better cryopreservation method, we tested the efficacy of cryovial monolayer vitrification method in comparison with that of needle immersed vitrification (NIV). Ovaries from 10 female kunming mice aged 6-8 weeks were cut into pieces and allocated into group A (cryovial monolayer vitrification method), group B (NIV method) and group C (fresh control). In group A, pieces of ovarian tissue were layered around the inner wall of cryovial as a monolayer; and in group B, pieces of ovarian tissue were pierced with a needle...
October 17, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29039070/the-evaluation-of-xenotransplantation-of-feline-ovarian-tissue-vitrified-by-needle-immersed-vitrification-technique-into-male-immunodeficient-mice
#19
Mürşide Ayşe Demirel, Duygu Baki Acar, Burcu Ekim, Ferda Topal Çelikkan, Kübra Karakaş Alkan, Seçkin Salar, Esra Atabenli Erdemli, Sinan Özkavukçu, Seda Sağlam Yar, Halit Kanca, Ayhan Baştan
In this study, the efficiency of the "Needle Immersed Vitrification" technique was tested on cryopreserved feline ovarian tissue. For vitrification, ovarian fragments (0.5-1.5 mm(2)) from each ovary were collected; the grafts were exposed to 7.5-15% ethylene glycol and 7.5-15% dimethyl sulfoxide at room temperature and stored in liquid nitrogen at least 1 week. Morphologic examinations, expression of genes such as B cell lymphoma 2, B-cell lymphoma-2-associated X protein, Bone morphogenetic protein 15, zone of polarizing activity, zona pellucida C protein and DNA (cytosine-5)-methyltransferase 1, ultrastructural analysis and viability tests were carried out from collected grafts...
October 16, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29032461/the-effect-of-ribose-pre-treatment-of-cortical-bone-on-%C3%AE-irradiation-sterilization-effectiveness
#20
Tarik Attia, Jindra Tupy, Dalal Asker, Benjamin Hatton, Marc Grynpas, Thomas Willett
Reconstruction of large skeletal defects is a significant and challenging issue. Tissue banks often use γ-irradiation (15-35 kGy) to sterilize bone allografts, which, however, drastically impairs the post-yield mechanical properties. In previous studies, we reported the development of a method that protects human bone collagen connectivity through ribose crosslinking while still undergoing γ-irradiation. Given these promising results, the next step was to determine if the presence of ribose within the bone tissue would interfere with the effectiveness of the γ-irradiation sterilization against bacteria...
October 14, 2017: Cell and Tissue Banking
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