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Journal of Veterinary Science

Seol-Hwa Yim, Tae-Wook Hahn, Hong-Gu Joo
We previously demonstrated that Bordetella bronchiseptica (B. bronchiseptica) antigen (Ag) showed high immunostimulatory effects on mouse bone marrow cells (BMs) while Mycoplasma hyopneumoniae (M. hyopneumoniae) Ag showed low. The focus of this study was if B. bronchiseptica Ag may enhance M. hyopneumoniae Ag-specific immune response, and also the host immune system can recognize both Ags. MTT assay revealed that each or both Ags did not significantly change the cell metabolic activity of BMs. Flow cytometry analysis using carboxyfluorescein succinimidyl ester presented that B...
September 23, 2016: Journal of Veterinary Science
Kiman Bang, Jae-Uk An, Woohyun Kim, Hee-Jin Dong, Junhyung Kim, Seongbeom Cho
Enterococcus spp. are normally present in the gastrointestinal tracts of animals and humans, but can cause opportunistic infections that can be transmitted to other animals or humans with integrated antibiotic resistance. To investigate whether this is a potential risk in military working dogs (MWDs), we analyzed antibiotic resistance patterns and genetic relatedness of Enterococcus spp. Isolated from fecal samples of MWDs of four different age groups. The isolation rates of Enterococcus spp., and specifically of E...
September 1, 2016: Journal of Veterinary Science
Hyeon-Joong Kim, Joon Young Kim, Byung-Hwan Lee, Sun-Hye Choi, Hyewon Rhim, Hyoung-Chun Kim, Seoung-Yob Ahn, Soon-Wuk Jeong, Minhee Jang, Ik-Hyun Cho, Seung-Yeol Nah
Ginseng gintonin is an exogenous ligand of lysophosphatidic acid (LPA) receptor. Accumulating evidence shows LPA helps a rapid recovery of corneal damage. The aim of this study was to evaluate the therapeutic efficacy of gintonin in a rabbit model of corneal damage. We investigated the signal transduction pathway of gintonin in human corneal epithelium (HCE) cells to elucidate the underlying molecular mechanism. We next evaluated the therapeutic effects of gintonin, using a rabbit model of corneal damage, by histochemical analysis...
September 1, 2016: Journal of Veterinary Science
Seung Hoon Lee, Yongsun Kim, Daeun Rhew, Ahyoung Kim, Kwang Rae Jo, Yongseok Yoon, Kyeung Uk Choi, Taeseong Jung, Wan Hee Kim, Oh-Kyeong Kweon
Heme oxygenase-1 (HO-1) is a stress-responsive enzyme that modulate immune response and oxidative stress associated with spinal cord injury (SCI). This study aimed to investigate neuronal regeneration by transplantation of mesenchymal stromal cells (MSCs) overexpressing HO-1. Canine MSCs overexpressing HO-1 were generated using a lentivirus packaging protocol. Eight beagle dogs with experimentally-induced SCI were GFP-labeled MSCs (MSC-GFP) and HO-1 overexpressed MSCs (MSC-HO-1) groups. MSCs (1 × 107 cells) were transplanted at 1 week after SCI...
September 1, 2016: Journal of Veterinary Science
Yeotaek Cheong, Changin Oh, Kunkyu Lee, Ki-Hyun Cho
Oral fluid analysis for monitoring herd is an interest in commercial pig farms in Korea. The aim of this study was to investigate the positive rates and correlation of eight pathogens associated with porcine respiratory disease complex (PRDC) by analyzing oral fluid samples from 214 pig groups from 56 commercial farms. The samples collected by rope-chewing method were analyzed with reverse-transcription polymerase chain reaction (RT-PCR) or standard PCR depending on microorganisms. The pathogens were divided into virus group and bacteria group...
September 1, 2016: Journal of Veterinary Science
Songsak Srisanga, Sunpetch Angkititrakul, Patcharee Sringam, Phuong T Le Ho, An T T Vo, Rungtip Chuanchuen
A total of 122 Salmonella isolates from dogs (n=113) and cats (n=9) comprising 32 serotypes were obtained from household dogs (n=250) and cats (n=50) during 2012-2015. The isolates were characterized by serotyping, examining phenotype and genotype of antimicrobial resistance and screening virulence genes. Serovars Weltevreden (15.6%) and Typhimurium (13.9%) were the most common serotypes. The majority of the isolates were multidrug resistant (43%). The dog isolates (12.3%) harbored class 1 integrons, of which the dfrA12-aadA2 cassette was most frequently identified (66...
September 1, 2016: Journal of Veterinary Science
Fang Yu, Roberta Rasotto, Hong Zhang, Shimin Pei, Bin Zhou, Xu Yang, Yipeng Jin, Di Zhang, Degui Lin
The Wnt signaling pathway and its key component β-catenin play critical roles in the development of diseases such as tumors in mammals. However, little is known about involvement of the Wnt/β-catenin signaling pathway in canine mammary tumors (CMTs). The present study detected the expression of 30 Wnt signaling pathway-related genes in CMTs, which are potentially useful for molecular diagnosis of CMTs and the development of new targeted therapies. Significant upregulation of DKK1, SFRP1, FZD3, β-catenin and LEF1 was detected in highly malignant CMTs compared to those in normal mammary gland tissues; while extremely significant upregulation of WNT5A was found in low malignant CMTs...
September 1, 2016: Journal of Veterinary Science
Jae-Gyun Choi, Jae-Min Kim, Dong-Wook Kang, Jung-Wan Choi, Jin Bong Park, Seong-Hun Ahn, Yeon Hee Ryu, Hyun-Woo Kim
The incidence of lung cancer has been rapidly increased and cancer patients at a later stage frequently suffer from the unbearable cancer-associated pain. However, pathophysiology of lung cancer pain has not been fully demonstrated due to lack of proper animal models. This study was designed to determine the effect of Lewis lung carcinoma (LLC) cell inoculation on formalin-induced pain behavior and spinal Fos expression in C57BL/6 mice. LLC cell (1.5X10⁵, 2.5X10⁵, 3.0X10⁵ or 5.0X10⁵) was inoculated into back or peri-sciatic nerve area...
September 1, 2016: Journal of Veterinary Science
Masahiro Yasuda, Jyunya Kawabata, Sayaka Akieda-Asai, Tetsuo Nasu, Yukari Date
Guanylyl cyclase C (GC-C) is a member of a family of enzymes that metabolize GTP to cGMP and was first identified as a receptor for heat-stable enterotoxin. Guanylin (GNY) has since been identified as an endogenous ligand for GC-C in the intestine of several species in mammals. The GNY/GC-C system regulates ion transportation and pH in the mucosa. Recently, it was reported that GC-C and GNY are involved in lipid metabolism in rat mesenteric adipose tissue macrophages. To examine the role of GC-C and GNY in lipid metabolism in cattle, we used a bovine mesenteric adipocyte primary culture system and a coculture system for bovine adipocytes and GNY-/GC-C-expressing macrophages...
September 1, 2016: Journal of Veterinary Science
Ziwei Zhang, Mingyu Bi, Jie Yang, Haidong Yao, Zhonghua Liu, Shiwen Xu
The aim of this study was to evaluate the influence of P deficiency on the morphological and functional characteristics of erythrocytes in cows. Forty Holstein-Friesian dairy cows in mid-lactation were randomly divided into two groups of 20 each and were fed either a low-P diet (0.03% P/kg DM) or a control diet (0.36% P/kg DM). Red blood cell indices results showed RBC and MCH decreased while MVC increased significantly (P<0.05) with P deficiency in cows; erythrocyte morphology showed erythrocyte destruction had occurred in P deficiency cows...
September 1, 2016: Journal of Veterinary Science
Yangho Jang, Kwangjick Lee, Seonjong Yun, Myoungheon Lee, Jaeyoung Song, Byungjoon Chang, Nong-Hoon Choe
The efficacy of six commercial disinfectants were evaluated using Salmonella enterica serovar Typhimurium under simulated natural conditions such as sub-zero temperature, short disinfecting time, and types of surface structure (uneven or smooth). We used a suspension-model test to determine the disinfecting efficacy under varying contact times (1, 5, 10, and 30 min) and temperatures (25°C, 4°C, 0°C, and -10°C). The bactericidal effect according to the surface structure was measured using a carrier-model test at 25°C and -10°C...
September 1, 2016: Journal of Veterinary Science
Peng Zhang, Qinghong Xue, Jing Ma, Jingjing RenN, Shuili Xia, Lu Zhang, Wenbin Wang, Supesh K Tikoo, Enqi Du
Bovine adenovirus type 3 (BAdV-3) is being developed as potential vehicles for vaccination. To this end, a more comprehensive understanding of BAdV-3 biology is essential. Here we focused on the role of pIX in BAdV-3 virions rescue post BAdV-3 genome transfection. Initially, pIX deletion or initiation codon mutation abolished the production of progeny virions suggested that pIX was essential for the rescue of BAdV-3 containing full-length genome. Moreover, a panel of mutant pIX, we confirmed that the conserved N-terminus and putative leucine zipper element (PLZP) were the essential domains, while the C-terminus following coiled-coil domain was non-essential...
September 1, 2016: Journal of Veterinary Science
Yawon Hwang, Dongwook Kim, Dongwoo Chang, Byeongwoo Ahn, Yun-Bae Kim, Gonhyung Kim
This is the first case report to describe the tumor regressive effect of systemic human neural stem cells (NSCs)/5-fluorocytosine (5-FC) therapy on canine metastatic lung tumor. The therapeutic effects appeared about two weeks after the 5-FC administration. Thoracic radiographs revealed a reduced number of lung nodules and decreased nodule size. However, there were no significant antitumor effects on primary lesions in the abdominal organs. In conclusion, human NSCs/5-FC prodrug therapy can secure patient quality of life with the same or more therapeutic effects and less side effects than other recommended chemotherapies...
August 10, 2016: Journal of Veterinary Science
Hye Kyung Baek, Pan Soo Kim, Ji Ae Song, Dong-Hwa Choi, Do Eun Kim, Seung Il Oh, Sang-Kyu Park, Sung-Jo Kim, Ki-Duk Song, In Koo Hwang, Hyung Seok Seo, Sun Shin Yi
Recently, we reported that Artemisia annua (AA) has anti-adipogenic properties in vitro and in vivo. Reduction of adipogenesis by AA treatment may dampen systemic inflammation and protect neurons from cytokine-induced damage. Therefore, the present study was performed to assess whether AA increases neuronal maturation by reducing inflammatory responses, such as those mediated by COX-2. Mice were fed normal chow or a high-fat diet with or without chronic daily oral administration of AA extract (0.2g/10mL/kg) for 4 weeks; then, changes in their hippocampal dentate gyri were measured with immunohistochemistry/immunofluorescence staining for BrdU, DCX, and NeuN, markers of neuronal maturation, and a quantitative Western blot for COX-2 and Iba-1, in order to assess correlations between systemic inflammation (Il-6) and food type...
August 10, 2016: Journal of Veterinary Science
Hye Kwon Kim, Kwang Soo Lyoo, Thi My Le Huynh, Hyoung Joon Moon, Van Giap Nguyen, Bong Kyun Park
There are high coincidences of PRRSV and PCV2 in the tissue distribution. This study established a duplex nested RT- PCR targeting the genomic RNA of type 2 PRRSV and mRNA of PCV2 in infected tissues. The method amplified discriminative bands of 347bp and 265bp specific for type 2 PRRSV and PCV2, respectively. The limit detection of the duplex nested RT- PCR was 10(1.5) TCID50/ml of type 2 PRRSV and 10(2) PCV2-infected cells/ml. The kappa statistic, measuring agreement between the methods, was 0.867, indicating a good level of agreement...
August 10, 2016: Journal of Veterinary Science
Fan Yang, Yiming Liu, Zhili Li, Yuqin Wang, Baobao Liu, Zhensheng Zhao, Bianhua Zhou, Guoyong Wang
Tissue distribution of marbofloxacin was studied in pigs after a single intramuscular injection at 2.5 mg/kg body weight. Samples of plasma, muscle, liver, kidney, heart, lung, and muscle at the injection site were randomly collected from five pigs at 2, 6, 10, 24, 48, 72, and 96 h after administration. Marbofloxacin concentrations were determined by high-performance liquid chromatography with ultraviolet detection, and then were subjected to non-compartmental analysis to get the kinetic parameters. The elimination half-live (t1/2λz) of marbofloxacin at injection site was 22...
August 10, 2016: Journal of Veterinary Science
Seong-In Lim, Yong Kwan Kim, Ji-Ae Lim, Song-Hee Han, Hee-Suk Hyun, Ki-Sun Kim, Bang-Hun Hyun, Jae-Jo Kim, In-Soo Cho, Jae-Young Song, Sung-Hyun Choi, Seung-Hoe Kim, Dong-Jun An
Classical swine fever (CSF), a highly contagious disease that affects domestic pigs and wild boar, has serious economic implications. The present study examined the virulence and transmission of strain YC11WB (isolated from a wild boar in 2011) in breeding wild boar. Virulence in domestic pigs was also examined. Based on the severe clinical signs and high mortality observed among breeding wild boar, the pathogenicity of strain YC11WB resembled that of typical acute CSF. Surprisingly, in contrast to strain SW03 (isolated from breeding pigs in 2003), strain YC11WB also showed both acute and strong virulence in breeding pigs...
August 10, 2016: Journal of Veterinary Science
Farruk M Lutful Kabir, Patricia DeInnocentes, Payal Agarwal, Christopher P Mill, David J Riese Ii, R Curtis Bird
Well characterized, stable, p16-defective canine mammary cancer (CMT) cell lines and normal canine mammary epithelial cells were used to investigate expression of the major breast cancer-specific hormone receptors estrogen receptor alpha (ER1) and progesterone receptor (PR) as well as luminal epithelial-specific proto-oncogenes encoding c-erbB-1 (epidermal growth factor receptor/EGFr), c-erbB-2/HER2, c-erbB-3 and c-erbB-4 receptors. This investigation developed and validated QrtPCR assays for each transcript to provide rapid assessment of breast cancer phenotype for canine cancers, based on ER1, PR and c-erbB-2/HER2 expression, similar to human disease...
August 10, 2016: Journal of Veterinary Science
Se Eun Kim, Tae Hyun Kim, Shin Ae Park, Won Tae Kim, Young Woo Park, Jae Sang Ahn, Manbok Jeong, Min-Young Kim, Kangmoon Seo
Matrix metalloproteinases (MMPs) are the main proteinase associated with periodontal tissue destruction and remodeling. Therefore, inhibition of the host-derived MMPs is accounted to play a key role in the prevention and reduction of the periodontitis progression. Horse chestnut (Aesculus hippocastanum L.) extracts had been used as a remedy for inflammatory disease, traditionally. This study was performed to assess the clinical effect of ALH-L1005 on the periodontitis as MMPs inhibitor. ALH-L1005 was obtained from horse chestnut leaf and the MMPs inhibitory activities were estimated...
August 10, 2016: Journal of Veterinary Science
Prangtip Phuwapattanachart, Naris Thengchaisri
The post-operative analgesic effects of firocoxib in ovariohysterectomized cats were observed. Twenty-four cats were divided into 3 groups: control (no medicine), firocoxib-1 (1 mg/kg/day) and firocoxib-3 (3 mg/kg/day). Colorado pain scale scores (CPSS), composite pain scores (CPS), and buccal mucosal bleeding times (BMBT) were recorded in blinded fashion before induction and 2, 5, 8, 24, 30, and 48 hours post-operation. The average post-operative CPSS (mean±SEM) from 2 to 48 hours post-operation in firocoxib-3 (0...
August 10, 2016: Journal of Veterinary Science
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