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Journal of Veterinary Science

Mwense Leya, Won Kyong Kim, Jeong Sang Cho, Eun-Chae Yu, Young-Jee Kim, Yoonhwan Yeo, Kwang-Soo Lyoo, Myeon-Sik Yang, Sang-Seop Han, John Hwa Lee, Dongseob Tark, Jin Hur, Bumseok Kim
Salmonella is an intracellular pathogen exhibiting a cellular infection mechanism similar to that of Brucella , making it a suitable choice for an anti- Brucella immune boost system. This study explores the efficacy of a Salmonella Typhimurium delivery-based combination vaccine for heterologous Brucella antigens (rBs) including Brucella lumazine synthase (BLS), proline racemase subunit A (PrpA), outer-membrane protein 19 (Omp19), and Cu/Zn superoxide dismutase (SOD) targeting brucellosis in goats. We inoculated the attenuated Salmonella delivery-based vaccine combination at two different subcutaneous inoculations; 5 × 109 CFU/ml (group B) and 5 × 10¹⁰ CFU/ml (group C) and challenged it with virulent Brucella abortus at 6 weeks post-immunization...
June 21, 2018: Journal of Veterinary Science
Hong-Tae Park, Hyun-Eui Park, Woo Bin Park, Suji Kim, Young-Hoon Jung, Han Sang Yoo
The aim of this study was to describe the genetic diversity of Mycobacterium avium subsp. paratuberculosis (MAP) obtained from individual cows in Korea. Twelve MAP-positive fecal DNA samples and 19 MAP isolates were obtained from 10 cattle herds located in 5 provinces in Korea. In addition to this, 5 MAP isolates were obtained from the Czech Republic and Slovakia, and 3 were from Australia were used for typing to compare with the domestic isolates. The most prevalent strains in Korea were the "bison-type" genotype (23 out of the 31 fecal DNA/isolates) and distributed nationwide...
June 21, 2018: Journal of Veterinary Science
Tomasz Grenda, Magdalena Grabczak, Zbigniew Sieradzki, Krzysztof Kwiatek, Krystyna Pohorecka, Marta Skubida, Andrzej Bober
The aim of this study was examination of 240 multifloral honey samples collected from Polish apiaries for C. botulinum occurrence. Honey was collected directly from apiaries after the extraction process. Samples were inoculated by using the dilution and centrifugation (DC) method. Suspected isolates were examined by using mouse bioassay, PCR and real-time PCR methods. Clostridium botulinum type A and B strains were detected in 5 (2%) of 240 examined honey samples. Strains were also detected which were phenotypically similar to C...
June 21, 2018: Journal of Veterinary Science
Michal Kyllar, Petr Čížek
Cranial cruciate ligament (CCL) rupture is one of the most common orthopaedic conditions in dogs. The pathogenesis of CCL rupture is not fully understood and remains to be elucidated. Several hypotheses have been proposed to explain the aetiology of these changes. The objective of this study was to investigate structural changes in the cranial cruciate ligament (CCL) in relation to the tibial plateau angle (TPA) and intercondylar notch (ICN) width in dogs. Fifty-five skeletally mature dogs were included in this study...
June 21, 2018: Journal of Veterinary Science
Seonghun Heo, Tae-Sung Hwang, Hee Chun Lee
The purpose of this study was to propose a standard of differentiation between normal dogs and patients with hyperadrenocorticism (HAC) by measuring the skin thickness using ultrasonography in small dogs. Significant changes in skin thickness of patients treated with prednisolone (PDS) or patients with HAC treated with trilostane were also evaluated. Skin thickness was retrospectively measured using three abdominal digital images obtained from small dog breeds weighing less than 15 kg undergoing abdominal ultrasonography...
June 21, 2018: Journal of Veterinary Science
Hoon-Seong Choi, Suk-Yun Kang, Dae-Hyun Roh, Sheu-Ran Choi, Yeonhee Ryu, Jang-Hern Lee
Respiratory inflammation is frequent and fatal pathologic state encountered in veterinary medicine. Although diluted bee venom (dBV) has potent anti-inflammatory effects, the clinical use of dBV is limited to several chronic inflammatory diseases. The present study was designed to propose the acupoint treatment of dBV as a novel therapeutic strategy for respiratory inflammatory disease. Experimental pleurisy was induced by injection of carrageenan into left pleural space in mouse. dBV was injected into a specific lung meridian acupoint (LU-5) or into arbitrary non-acupoint located near the midline of the back in mouse...
June 21, 2018: Journal of Veterinary Science
Hyun-Young Park, Yu-Gyeong Cho, Young-Won Lee, Ho-Jung Choi
The feasibility of computed tomography (CT) to identify common bile duct (CBD) and comparison with ultrasonography results were evaluated in normal beagle dogs and dogs without hepatobiliary and pancreatic diseases. In addition, CBD diameters obtained from CT at the level of porta hepatis and duodenal papilla level in dogs with underlying diseases which may cause cholestasis. US is useful modality to estimate gallbladder volume, because ejection fraction and CBD diameter were not significantly different with the results of CT...
June 21, 2018: Journal of Veterinary Science
Min Jung Kim, Hyun Ju Oh, Sun Young Hwang, Tai Young Hur, Byeong Chun Lee
Dogs can serve human society in various ways by working at tasks based on their superior olfactory sensitivity. However, it has been reported that only about half of all trained dogs qualified as working dogs through conventional breeding management, because proper temperament and health are needed in addition to their innate scent detection ability. To overcome this low efficiency of producing working dogs and to reduce the enormous costs of maintaining unqualified dogs, somatic cell nuclear transfer has been applied to propagate working dogs...
June 21, 2018: Journal of Veterinary Science
Huimin Zhao, Junyu Nie, Xiangxing Zhu, Yangqing Lu, Xingwei Liang, Huiyan Xu, Xiaogan Yang, Yunkai Zhang, Kehuan Lu, Shengsheng Lu
In this study, we attempted to establish a culture system for in vitro spermatogenesis from spermatogonial stem cells (SSCs) of Bama Mini-pig. The dissociated testicular cells from one-month pig were co-cultured to mimic in vivo spermatogenesis. The testicular cells were seeded in Minimum Essential Media alpha (α-MEM) supplemented with KnockoutTM Serum Replacement (KSR). Three-dimensional (3D) colonies were formed after 10 days culture. The colonies showed positive staining for SSC-associated markers such as UCHL1, PLZF, THY1, OCT4, Dolichos biflorus agglutinin (DBA) and alkaline phosphatase (AP)...
June 21, 2018: Journal of Veterinary Science
Guehwan Jang, Seong-Hee Kim, Yoo Jin Lee, Seungjoon Kim, Du Sik Lee, Kyoung-Ki Lee, Changhee Lee
Porcine deltacoronavirus (PDCoV) has emerged in several pig-raising countries and has also been a causative pathogen associated with diarrheal diseases in South Korea since 2014. In the present study, we were able to isolate and cultivate one Korean PDCoV strain in cell culture and further investigated its pathogenicity. PDCoV-inoculated piglets showed watery diarrhea accompanied by acute enteritis in the natural host. Sequencing analysis demonstrated genetic stability of KNU16-07 for at least thirty serial passages...
April 26, 2018: Journal of Veterinary Science
Ziqiang Zhang, Yumei Liu, Xuemin Zhu, Lan Wei, Jiamin Zhu, Ke Shi, Guotao Wang, Li Pan
Previous studies have shown that sciatic nerve has neurotrophic activity, and nerve regeneration, differentiation, axon outgrowth can be modulated by different sciatic nerve preparations. However, a number of animals have to be sacrificed to get sciatic nerves for making sciatic nerve preparations. Some studies have demonstrated that the role of sciatic nerve preparations in neural differentiation depends on the neurotrophins that schwann cells secrete, and these factors are highly conserved between different species...
April 26, 2018: Journal of Veterinary Science
Heidrun Gehlen, Lisa-Marie Bildheim
An effect of aging on cardiac morphology and function has been shown in humans. In horses, cardiac wall-motion analysis has not been performed using two-dimensional speckle tracking (2D-ST) so far. Our study included 57 horses of different warmblood breeds between 3 and 30 years of age. Age had a significant influence on left ventricular (LVFW) systolic strain rate (P ≤ 0.05) and early diastolic relaxation (P ≤ 0.01). In the interventricular septum (IVS), systolic (P ≤ 0.01) and late diastolic (P ≤ 0...
April 26, 2018: Journal of Veterinary Science
Tomohiro Kondo, Suguru Yoshida, Hiroaki Nagai, Ai Takeshita, Masaki Mino, Hiroshi Morioka, Takayuki Nakajima, Ken Takeshi Kusakabe, Toshiya Okada
In Mongolian gerbils, bilateral common carotid artery occlusion (BCCAO) for several minutes induces ischemia and delayed neuronal cell death in the Cornet d'Ammon 1 (CA1) region of the hippocampus by their incomplete circle of Willis. Neuronal cell death in the hippocapus and changes in behavior were examined after BCCAO was performed for 5 minutes in the gerbils. One day after BCCAO, the pyramidal neurons of the CA1 region of the hippocampus showed degenerative changes (clumped chromatin of the nuclei). Five days and 10 days after BCCAO, extensive neuronal cell death was observed in the hippocampal CA1 region...
April 26, 2018: Journal of Veterinary Science
Rachel F Madera, Lihua Wang, Wenjie Gong, Yulia Burakova, Sterling Buist, Jerome Nietfeld, Jamie Henningson, Ada G Cino-Ozuna, Changchun Tu, Jishu Shi
Highly contagious classical swine fever (CSF) remains a major trade and health problem in the pig industry, resulting in large economic losses worldwide. In CSF-endemic countries, attenuated CSF virus (CSFV) vaccines have been routinely used to control the disease. However, eradication of CSFV in a geographical area would require permanent reduction to zero presence of the virus. It is therefore of paramount importance to develop a safe, potent, and non-infectious CSF vaccine. We have previously reported on a cost-effective CSF E2 subunit vaccine, KNB-E2, which can protect against CSF symptoms in a single dose containing 75 µg of recombinant CSFV glycoprotein E2...
May 31, 2018: Journal of Veterinary Science
Seung-Chul Lee, Sunhee Lee, Gun-Woo Yoo, Hwan-Won Choi, Yun-Hee Noh, Chang Eon Park, Jae-Ho Shin, In-Joong Yoon, Shien-Young Kang, Changhee Lee
The porcine reproductive and respiratory syndrome virus (PRRSV) is a globally ubiquitous swine viral pathogen that causes major economic losses worldwide. We previously reported an over-attenuated phenotype of cell-adapted PRRSV strain CA-2-P100 in vivo . In the present study, CA-2-P100 was serially propagated in cultured porcine alveolar macrophage (PAM) cells for up to 20 passages to obtain the derivative strain CA-2-MP120. Animal inoculation studies revealed that both CA-2-P100 and CA-2-MP120 had decreased virulence, eliciting weight gains, body temperatures, and histopathologic lesions similar to those in the negative control group...
May 31, 2018: Journal of Veterinary Science
Jung-Whan Chon, Kun-Ho Seo, Dongryeoul Bae, Ji-Hee Park, Saeed Khan, Kidon Sung
Clostridium perfringens causes diarrhea and other diseases in animals and humans. We investigated the prevalence, toxin gene profiles, and antibiotic resistance of C. perfringens isolated from diarrheic dogs (DD) and non-diarrheic dogs (ND) in two animal hospitals in Seoul, Korea. Fecal samples were collected from clinically DD (n = 49) and ND (n = 34). C. perfringens was isolated from 31 of 49 DD (63.3%) and 21 of 34 ND dogs (61.8%). All C. perfringens strains were positive for the α toxin gene, but not for the β, ε, or ι toxin genes; therefore, all strains were identified as type A C...
May 31, 2018: Journal of Veterinary Science
Pedro Melendez, Veronica Gomez, Hans Bothe, Francisco Rodriguez, Juan Velez, Hernando Lopez, Julian Bartolome, Louis Archbald
The objective of this study was to compare ovulation rate, number of large ovarian follicles, and concentrations of plasma progesterone (P4) and non-esterified fatty acids (NEFA) between lame (n = 10) and non-lame (n = 10) lactating Holstein cows. The study was conducted in an organic dairy farm, and cows were evaluated by undertaking ultrasonography and blood sampling every 3 days from 30 days postpartum for a period of 34 days. Cows which became lame during the first 30 days postpartum experienced a lower ovulation rate determined by the presence of a corpus luteum (50% presence for lame cows and 100% for non-lame cows, p ≤ 0...
May 31, 2018: Journal of Veterinary Science
Katharine Roque, Kyung Min Shin, Ji Hoon Jo, Gyeong Dong Lim, Eun Seob Song, So Jung Shin, Ravi Gautam, Jae Hee Lee, Yeon Gyeong Kim, Ah Rang Cho, Chang Yul Kim, Hyun Ji Kim, Myung Sook Lee, Hyeong-Geu Oh, Byung-Chul Lee, Jung Hee Kim, Kwang-Ho Kim, Hyun Kyu Jeong, Hyoung Ah Kim, Yong Heo
Indoor animal husbandry environments are inevitably contaminated with endotoxins. Endotoxin exposure is associated with various inflammatory illnesses in animals. This cross-sectional study evaluated the relationship between the degree of endotoxin exposure and the cellular and humoral immune profiles of fattening pigs. Blood samples were taken from the jugular vein of 47 pigs from ten pig farms in Korea. Whole blood cell counts and plasma immunoglobulin (Ig) classes were determined. Peripheral-blood mononuclear cells were stimulated in vitro with concanavalin A for 48 h, and cytokines released into culture supernatants were measured...
May 31, 2018: Journal of Veterinary Science
Jonathan Lalsiamthara, Gayeon Won, John Hwa Lee
An anti- Brucella vaccine candidate comprised of purified Brucella lipopolysaccharide (LPS) and a cocktail of four Salmonella Typhimurium (ST)- Brucella vectors was reported previously. Each vector constitutively expressed highly conserved Brucella antigens (rB), viz. , lumazine synthase (BLS), proline racemase subunit A, outer membrane protein-19 (Omp19), and Cu-Zn superoxide dismutase (SOD). The present study determined a relative level of protection conferred by each single strain. Upon virulent challenge, the challenge strain was recovered most abundantly in non-immunized control mice, with the ST-Omp19-, ST-BLS-, LPS-, and ST-SOD-immunized mice showing much less burden...
May 31, 2018: Journal of Veterinary Science
Dan Wang, Shuhong Sun, Mohammad Heidari
To provide insights into the role of innate immune responses in vaccine-mediated protection, we investigated the effect of Marek's disease (MD) vaccine, CVI988/Rispens, on the expression patterns of selected genes associated with activation of macrophages in MD-resistant and MD-susceptible chicken lines. Upregulation of interferon γ, interleukin (IL)-1β, IL-8, and IL-12 at different days post-inoculation (dpi) revealed activation of macrophages in both chicken lines. A strong immune response was induced in cecal tonsils of the susceptible line at 5 dpi...
May 31, 2018: Journal of Veterinary Science
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