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Journal of Veterinary Science

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https://www.readbyqxmd.com/read/28927257/differential-and-correlated-expression-of-p16-p21-p27-p38-in-mammary-gland-tumors-of-aged-dogs
#1
Hyun-Woo Kim, Jong-Il Shin, Byung-Joon Seung, Jung-Hyung Ju, Jung-Hyang Sur
The inhibitory effect of neutering on mammary gland tumor development in dogs is well known. However, we found that the effect of neutering on tumor malignancy may be altered by aging. Therefore, we aimed to characterize mammary tumors in aged dogs by analyzing the expression of cellular senescence markers, from the viewpoint of senescence. The expression of p16, p38, p21, and p27 antibodies, which are senescence-associated markers, was detected in canine mammary tumors of aged dogs via immunohistochemistry...
September 20, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28927256/comparison-of-as-pcr-crs-pcr-and-pcr-pira-methods-used-for-screening-cvm-carriers-in-holstein-cattle
#2
Kozet Avanus, Ahmet Altinel
Complex vertebral malformation (CVM) is an inherited and autosomal recessive disorder of Holstein cattle. The aim of this study was comparing sensitivity, specificity, positive and negative predictive values, accuracy and rapidity of three methods; allele specific PCR (AS-PCR), created restriction site PCR (CRS-PCR) and PCR with primer introduced restriction analysis (PCR-PIRA) that were used in identification of CVM carriers in Holstein cattle population. In order to screen G>T mutation in solute carrier family 35 member A3 (SLC35A3) gene DNA sequencing was used as gold standard method...
September 20, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28927255/innate-immune-response-of-bovine-mammary-epithelial-cells-to-mycoplasma-bovis
#3
Satoshi Gondaira, Hidetoshi Higuchi, Hidetomo Iwano, Koji Nishi, Takanori Nebu, Keiichi Nakajima, Hajime Nagahata
Mycoplasma species are contagious bacteria and Mycoplasma bovis mastitis is a serious productivity problem on dairy farms. Bovine mammary epithelial cells (bMEC) play an important role in the elimination of pathogens, but the effect of M. bovis on bMEC has not been fully clarified. To better understand the immune response against intramammary infection by M. bovis, we used microarray analysis to examine and profile mRNA expression in bMEC after stimulation with M. bovis. We also compared the effects of M. bovis, Staphylococcus aureus and Escherichia coli on immune-related mRNA expression in bMEC...
September 20, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28927254/integrated-analysis-of-microrna-and-mrna-expression-in-peripheral-blood-leukocytes-of-warmblood-horses-before-and-after-exercise
#4
Hang-Ah Kim, Myung-Chul Kim, Na-Yon Kim, Doug-Young Ryu, Hong-Seok Lee, Yongbaek Kim
Exercise capacity is a valuable trait in horses, and it has been used as one of their selection criteria. Although exercise affects molecular homeostasis and adaptation in horses, the mechanisms underlying these effects are not fully understood. This study was carried out to identify changes in the blood profiles of microRNAs (miRNAs) and mRNAs induced by exercise in horse leukocytes. Total RNAs isolated from the peripheral blood leukocytes of four Warmblood horses before and after exercise were subjected to next-generation sequencing (NGS) and microarray analyses to determine the miRNA and mRNA expression profiles, respectively...
September 20, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28927253/comparative-aspects-of-microrna-expression-in-canine-and-human-cancer
#5
REVIEW
Kabiru Sahabi, Gayathri Thevi Selvarajah, Rasedee Abdullah, Cheah Yoke Kqueen, Tan Geok Chin
MicroRNAs (miRNAs) are important players in all biological pathways in multicellular organisms. Over 1,400 human miRNAs have been identified, and many are conserved among vertebrates and invertebrates. MicroRNA regulation is the most abundant mode of post-transcriptional gene regulation. MicroRNAs that are involved in the initiation and progression of cancers are termed oncomiRs, several of which have been identified in canine and human cancer. Similarly, several miRNAs have been reported to be down-regulated in cancers of the two species...
September 20, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28693313/comparative-proteomic-analysis-of-the-outer-membrane-protein-43-omp43-deficient-bartonella-henselae
#6
Jun-Gu Kang, Hee-Woo Lee, Sungjin Ko, Joon-Seok Chae
Outer membrane proteins (OMPs) of gram-negative bacteria constitute the first line of defense that protects the cells against environmental stresses including chemical, biophysical, and biological attacks. Although 43-kDa outer membrane protein (OMP43) is major porin protein among the Bartonella henselae-derived OMPs, its function remains unknown. In this study, OMP43-deficient mutant B. henselae (Δomp43) was generated to investigate function of OMP43. Interestingly, Δomp43 showed a weaker proliferative ability compared to wild type (WT) of B...
July 10, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28693312/in-vitro-treatment-of-lipopolysaccharide-increases-the-invasion-of-pasteurella-multocida-serotype-b-2-into-bovine-aortic-endothelial-cell
#7
Sengkar Yap, Zunita Zakaria, Siti Sarah Othman, Abdul Rahman Omar
Pasteurella multocida serotype B:2 causes haemorrhagic septicaemia (HS) in cattle and buffaloes. The invasion mechanism of the bacterium to invade the bloodstream is unclear. This study aims to characterize the effects of immunomodulatory molecules, namely dexamethasone and lipopolysaccharide treatments, on the invasion efficiency of P. multocida serotype B:2 towards bovine aortic endothelial cells (BAECs) and the involvement of actin microfilament in the invasion mechanism. The results imply that treatment of BAECs with lipopolysaccharide at 100 ng/mL for 24 h significantly increases the intracellular bacteria number per cell (P<0...
July 10, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28693311/piglet-colibacillosis-diagnosis-by-multiplex-pcr-and-immunohistochemistry-of-paraffin-embedded-tissues
#8
Cíntia De Lorenzo, Caroline P de Andrade, Verônica S L Machado, Matheus V Bianchi, Veronica M Rolim, Raquel A S Cruz, David Driemeier
Enterotoxigenic Escherichia coli (ETEC) causes diarrhea in pigs, a condition termed colibacillosis, in which these bacteria should adhere and produce enterotoxins. The aim of this study was to optimize multiplex polymerase chain reaction (PCR) and immunohistochemistry (IHC) analyses of paraffin-embedded material to detect pathogenic strains of E. coli causing colibacillosis in pigs, which requires the expression of at least one fimbria and one toxin of the agent. Multiplex PCR was optimized for fimbriae (F18, F4, F6, F5, and F41) and toxins [types A and B heat-stable toxins (STaP and STb), heat-labile toxin (LT), and type 2 Shiga toxin (STx2e)], and IHC was optimized for an anti-E...
July 10, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28693310/tibial-dyschondroplasia-is-highly-related-to-suppression-of-the-hypoxia-inducible-factor-hif-1%C3%A9-2%C3%A9-and-3%C3%A9-expressions-in-chickens
#9
Shucheng Huang, Mujeeb Ur Rehman, Gang Qiu, Houqiang Luo, Muhammad Kashif Iqbal, Hui Zhang, Khalid Mehmood, Jiakui Li
Tibetan chickens (TBCs) live at high altitude hypoxic environment. None of the Tibial dyschondroplasia (TD) cases had been reported in TBCs, which is commonly seen in commercial broilers characterized by lameness. The underlying mechanism remains unclear. Hypoxia inducible factors (HIFs) are important regulators of cellular adaptation to hypoxic conditions. Here we investigated the role of HIF-1ɑ, -2ɑ, and -3ɑ in hypoxia and thiram-induced TD and their effect on tibial growth plate development in Acres chickens (AACs) and TBCs...
July 10, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28693309/variation-of-canine-vertebral-bone-architecture-in-computed-tomography
#10
Byunggyu Cheon, Seungjo Park, Sang-Kwon Lee, Jun-Gyu Park, Kyoung-Oh Cho, Jihye Choi
Focal vertebral bone density changes were assessed in vertebral CT images from the clinically healthy dogs, without diseases that affect bone density. The number, location, and density of the lesion were determined. A total of 429 vertebral CT images from 20 dogs were reviewed, and 99 focal vertebral changes were identified in 14 dogs. Focal vertebral bone density changes were mainly found in thoracic vertebra (29.5%) as hyperattenuating (86.9%) lesions. All focal vertebral changes were observed at the vertebral body, except for a single hyperattenuating change in one thoracic transverse process...
July 10, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28693308/isolation-and-characterization-of-a-new-porcine-epidemic-diarrhea-virus-variant-that-occurred-in-korea-in-2014
#11
Dong-Kun Yang, Ha-Hyun Kim, Seong-Hun Lee, Soon-Seek Yoon, Joong-Won Park, In-Soo Cho
Porcine epidemic diarrhea (PED) has caused significant economic losses in the swine industry, and another epidemic occurred recently in Korea in 2014. Isolating and culturing the PED virus (PEDV) allow investigations into its pathogenesis and development of vaccines and diagnostic assays. In this study, we successfully isolated two PEDV isolates (QIAP1401 and QIAP1402) from naturally infected piglets in Jeju-do. Viral propagation was confirmed in Vero cells according to cytopathic effects, immunofluorescence assay, reverse transcription-polymerase chain reaction and electron microscopic analyses...
July 10, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28693307/polymorphisms-in-the-serotonin-transporter-protein-gene-in-maltese-dogs-with-degenerative-mitral-valve-disease
#12
Chang-Min Lee, Jae-Ik Han, Min-Hee Kang, Seung-Gon Kim, Hee-Myung Park
Degenerative mitral valve disease (DMVD) is the most commonly acquired cardiac disease in dogs. This study was conducted to evaluate the relationship between genetic variations in serotonin transporter (SERT) gene of Maltese dogs and DMVD. Twenty client-owned DMVD Maltese dogs and 10 healthy control dogs. Genomic DNA was extracted from blood samples collected from these dogs, and each exon of the SERT gene was amplified using polymerase chain reaction. The resulting genetic sequences were aligned and analyzed for variations by using the reference sequences; the predicted secondary structures of these variations were modeled and cross-verified by computational methods...
July 10, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28693306/tannic-acid-mediated-immune-activation-attenuates-brucella-abortus-infection-in-mice
#13
Alisha Wehdnesday Bernardo Reyes, Huynh Tan Hop, Lauren Togonon Arayan, Tran Xuan Ngoc Huy, Wongi Min, Hu Jang Lee, Hong Hee Chang, Suk Kim
Brucellosis is an emerging infectious disease affecting humans and animals. In this study, we investigated the in vitro and in vivo effects of tannic acid (TA) against Brucella (B.) abortus infection. Reduced F-actin polymerization and MAPKs (ERK 1/2 and p38α) phosphorylation were observed in TA-treated cells as compared with control cells after infection. The mice were infected via intraperitoneal route and were orally given with TA or PBS for 14 days. The weights of the spleens from TA-treated and control mice were not different, however the splenic proliferation of B...
July 10, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28693305/comparison-the-characteristics-of-canine-adipose-tissue-derived-mesenchymal-stem-cells-extracted-from-different-sites-and-at-different-passage-numbers
#14
Kevin M Yaneselli, Cristiana Palma Kuhl, Paula Barros Terraciano, Fernanda dos Santos de Oliveira, Sabrina Beal Pizzato, Kamila Pazza, Alessandra Bileski Magrisso, Vanessa Torman, Analía Rial, María Moreno, Silvia Llambí, Elizabeth Cirne Lima, Jacqueline Maisonnave
Mesenchymal Stem Cells (MSCs) have attractive characteristics for therapy in animal models and veterinary medicine, due to the capacity of inducing tissue regeneration and immunomodulation. The objective of this study was to evaluate the differences between canine adipose tissue-derived MSCs (AD-MSCs) extracted from subcutaneous (Sc) and visceral (Vs) sites. Surface antigenic markers, in vitro differentiation and mineralized matrix quantification of AD-MSCs in different passages (P4, P6 and P8) were studied...
July 10, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28693304/prepubertal-growth-and-single-nucleotide-polymorphism-analysis-of-the-growth-hormone-gene-of-holstein-calves-born-smaller
#15
Younghye Ro, Woojae Choi, Hoyung Kim, Hojin Jang, Hoseon Lee, Yoonseok Lee, Danil Kim
Holstein calves weighing less than 20 kg at birth have been born, but no studies on small Holstein calves have been reported in South Korea. We raised small calves with age-matched normal-birth weight Holstein calves and measured body weight before puberty. Three single nucleotide polymorphisms (SNPs) of the growth hormone gene were also analyzed. Up to 10 months of age, low-birth weight calves remained undersized compared to normal calves. In exon 5 of the growth hormone, the SNP was detected in some small calves; however, this did not appear to be the only factor inducing low-birth weight and slow growth...
July 10, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28693303/a-new-recombined-prrsv-virulent-strain-in-china
#16
Jian-Guo Dong, Lin-Yang Yu, Pei-Pei Wang, Le-Yi Zhang, Yan-Ling Liu, Peng-Shuai Liang, Chang-Xu Song
Porcine reproductive and respiratory syndrome (PRRS) is one of the most important swine diseases worldwide. In the present study, a new virulent strain of PRRS virus(PRRSV) GDsg was isolated in Guangdong province of China, which caused high fever, high morbidity and high mortality for sow and piglets. The genome of this new strain is 15,413 nucleotides(nt) in length and comparative analysis revealed that GDsg shared 82.4% to 94% identity with type 2 PRRSV strains, but only 61.5% with type 1 PRRSV strain-Lelystad Virus, Phylogenetic analysis indicated that type 2 PRRSV isolates formed five subgenotypes (I, II, III, IV, V), which was represented by NADC30, VR-2332, GM2, CH-1a and HuN4, respectively...
July 10, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28693302/packaging-of-rvfv-pseudoviruses-and-establishment-of-a-neutralization-assay-method
#17
Yuetao Li, Yongkun Zhao, Cuiling Wang, Xuexing Zheng, Hualei Wang, Weiwei Gai, Hongli Jin, Feihu Yan, Boning Qiu, Yuwei Gao, Nan Li, Songtao Yang, Xianzhu Xia
Rift Valley fever (RVF) is an acute, febrile zoonotic disease that is caused by the RVF virus (RVFV). RVF is mainly prevalent on the Arabian Peninsula, the African continent, and several islands in the Indian Ocean near southeast Africa. RVFV has been classified by the World Organisation for Animal Health (OIE) as a category A pathogen. To avoid the biological safety concerns associated with use of the pathogen in RVFV neutralization assays, the present study explored and established an RVFV pseudovirus-based neutralization assay...
July 10, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28693301/serological-and-molecular-epidemiology-of-japanese-encephalitis-virus-infections-in-swine-herds-in-china-2006-2012
#18
Chunxia Chai, Qiao Wang, Sanjie Cao, Qin Zhao, Yiping Wen, Xiaobo Huang, Xintian Wen, Qiguai Yan, Xiaoping Ma, Rui Wu
Japanese encephalitis virus (JEV) is a mosquito-borne, zoonotic flavivirus causing viral encephalitis in humans and reproductive disorder in pig. JEV is widely prevalent throughout China in human, however, studies on JEV spatiotemporal analysis in Chinese swine herds have not been explored. Here, we present our serological and molecular epidemiological findings of the prevalence of JEV infections among swine herds in China, which suggest that JEV infections are extensively widespread and genotype I and III strains co-exist...
July 10, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28693300/investigation-of-bovine-tuberculosis-outbreaks-using-the-trace-back-system-and-molecular-typing-in-korean-hanwoo-beef-cattle
#19
Bok Kyung Ku, Bo-Young Jeon, Jae Myung Kim, Young-Boo Jang, Hyeyoung Lee, Jae Young Choi, Suk Chan Jung, Hyang-Mi Nam, Hun Park, Sang-Nae Cho
Bovine tuberculosis is a chronic contagious disease responsible for major agricultural economic losses. Abattoir monitoring and trace-back systems are an appropriate method to control bovine tuberculosis, particularly in beef cattle. In the present study, a trace-back system was applied to bovine tuberculosis outbreaks of Korean native Hanwoo beef cattle. Bovine tuberculosis was detected in three index beef cattle during abattoir monitoring in Jeonbuk Province, Korea, and the original herds were traced back from each index cattle...
July 10, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28693299/magnetic-resonance-imaging-characteristics-of-ischemic-brain-infarction-according-to-time-passage-in-a-canine-stroke-model
#20
Sooyoung Choi, Daji Noh, Youngwhan Kim, Inseong Jeong, Hojung Choi, Youngwon Lee, Kija Lee
This study describes magnetic resonance imaging findings and changes in lateral ventricular size according to time passage of canine ischemic stroke model. T1- and T2-weighted (T1W, T2W) imaging and fluid-attenuated inversion recovery (FLAIR) sequence were performed at 3 h and 3, 8, and 35 days after brain infarct induction. Diffusion-weighted imaging (DWI) and apparent diffusion coefficient mapping were obtained at 8 and 35 days. A total of 29 brain lesions were induced successfully in 12 of 14 beagle dogs...
July 10, 2017: Journal of Veterinary Science
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