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Journal of Veterinary Science

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https://www.readbyqxmd.com/read/28385014/mechanisms-of-quinolone-resistance-in-escherichia-coli-isolated-from-companion-animals-pet-owners-and-non-pet-owners
#1
Yeon Soo Chung, Yoon Sung Hu, Sook Shin, Suk Kyung Lim, Soo Jin Yang, Yong Ho Park, Kun Taek Park
The present study investigated the prevalence and the mechanisms of fluoroquinolone (FQ)/quinolone (Q) resistance in Escherichia coli isolates from companion animals, pet-owners, and non-pet owners. A total of 63 E. coli isolates were collected from 104 anal swab samples; 27 nalidixic acid (NA)-resistant isolates were identified. Of these, 10 showed ciprofloxacin (CIP) resistance. Plasmid-mediated Q resistance gene was detected in an isolate. Increased efflux pump activity, as measured by organic solvent tolerance assay, was detected in 18 isolates (66...
April 6, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28385013/quantitative-evaluation-of-renal-parenchymal-perfusion-using-contrast-enhanced-ultrasonography-in-renal-ischemia-reperfusion-injury-in-dogs
#2
Gahyun Lee, Sunghoon Jeon, Sang-Kwon Lee, Byunggyu Cheon, Sohyeon Moon, Jun-Gyu Park, Kyoung-Oh Cho, Jihye Choi
This study evaluated whether renal perfusion changes can be noninvasively estimated using contrast-enhanced ultrasonography (CEUS) in renal ischemia-reperfusion injury and investigated the correlation between renal perfusion measured by CEUS and necrosis and apoptosis of renal tubular epithelial cell. In six dogs with experimentally induced renal ischemia-reperfusion injury, changes in time to peak intensity, peak intensity and area under the curve were measured on CEUS. Peak intensity and area under the curve of the renal cortex began to decrease by about 20% compared to baseline from day 1, and reached its lowest level, about 50% to baseline, on day 4...
April 6, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28385012/eosinophilic-encephalomyelitis-in-horses-caused-by-protostrongylid-parasites
#3
Eun-Jung Bak, Young-Hwa Jean, Gye-Hyeong Woo
Four thoroughbred horses showing lameness, ataxia, circling, depression, recumbency, and seizures, were submitted. The horses had grossly weak to dark red appearances and foci in the central nervous system. Multifocal to coalescing eosinophilic necrotizing encephalomyelitis was observed histologically in the CNS as well as intact or degenerated nematodes. Nematodes had polymyarian-coelomyarian musculature, a smooth thin cuticle, and intestines lined by multinucleated cells with microvilli. These traits suggested the nematodes belonged to the family Protostrongylidae, which includes P...
April 6, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28385011/pharmacokinetics-of-tilmicosin-in-healthy-pigs-and-in-pigs-experimentally-infected-with-haemophilus-parasuis
#4
Ling Zhang, Li Zhao, Yonghong Liu, Junfeng Liu, Xianqiang Li
A comparative in vivo pharmacokinetic (PK) study of tilmicosin (TIL) was conducted in 6 crossbred healthy pigs and 6 crossbred pigs infected with Haemophilus parasuis (H. parasuis) following oral administration of a single dose of 40 mg/kg. The infected model was established by intranasal inoculation and confirmed by clinical signs, blood biochemical and microscopic examination. TIL concentrations in plasma were determined by a validated high-performance liquid chromatography (HPLC) method with ultra violet detection at a wavelength of 285 nm and PK parameters were calculated by using the Winnonlin software...
April 6, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28385010/coactosin-like-protein-1-inhibits-the-neuronal-migration-during-mouse-corticogenesis
#5
Guohong Li, Yupeng Yin, Jiong Chen, Yanle Fan, Juhong Ma, Yingxue Huang, Chen Chen, Pengxiu Dai, Shulin Chen, Shanting Zhao
Coactosin-like protein-1 (Cotl1), a member of the actin-depolymerizing factor (ADF)/cofilin family, was first purified from the soluble fraction of Dictyostelium discoideum cells. Neuronal migration requires cytoskeletal remodeling and actin regulation. Although Cotl1 strongly binds to F-actin, the role of Cotl1 in neuronal migration remains unknown. Here, we revealed that Cotl1 overexpression impaired the migration of both early- and late-born neurons during mouse corticogenesis. Moreover, Cotl1 overexpression delayed, rather than blocked, neuronal migration in late-born neurons...
April 6, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28385009/inhibitory-effects-of-resveratrol-on-hepatitis-b-virus-x-protein-hbx-induced-hepatocellular-carcinoma-hcc
#6
Seungmo Park, Jihae Lim, Jong Rhan Kim, Seongbeom Cho
Liver cancer occurs very frequently worldwide and hepatocellular carcinoma (HCC) accounts for more than 80% of total primary liver cancer cases. Here, the anticarcinogenic effects of resveratrol against hepatitis B virus (HBV)-induced HCC were investigated using HBV X-protein-overexpressing Huh7 (Huh7-HBx) human hepatoma cells. MTT assay showed that resveratrol decreased cell viability. Fluorescence-activated cell sorter analysis showed that it induced G1 cell cycle arrest without increasing the sub-G1 phase cell population...
April 6, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28385008/use-of-stem-cell-sheets-expressing-bone-morphogenetic-protein-7-in-the-management-of-a-nonunion-radial-fracture-in-a-toy-poodle
#7
Jaeyong Song, Yongsun Kim, Oh-Kyeong Kweon, Byung-Jae Kang
A 12-year-old castrated Toy Poodle was referred to Kangwon National University Animal Hospital with an oligotrophic nonunion fracture in the left distal 1/3 of the radius and an intact ulna. After fixation using a locking plate and screws, adipose-derived mesenchymal stem cell sheets expressing bone morphogenetic protein 7 (BMP-7) were transplanted to the fracture site to enhance the healing activity. The fracture healed at 9 weeks after surgery. In the present case, the mesenchymal stem cell sheets expressing BMP-7 promoted bone regeneration and healing in a nonunion fracture...
April 6, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28385007/experimental-infection-of-dogs-with-highly-pathogenic-avian-influenza-virus-h5n8
#8
Seong-Su Yuk, Dong-Hun Lee, Jae-Keun Park, Eredene-Ochir To, Jung-Hoon Kwon, Jin-Yong Noh, Chang-Seon Song
During the highly pathogenic avian influenza (HPAI) H5N8 outbreak in South Korea, a dog reared in layer farm contaminated by the virus was reported seropositive for HPAI H5N8. In order to investigate the possibility of the adaptation and transmission of HPAI H5N8 to dogs, we inoculated the H5N8 to dogs in experimental condition. Weak viral genes in nasal swabs and seroconversions were detected even in contact dogs. Although the H5N8 virus doesn't seem to adapt to canine species fully, these results suggest that surveillance on farm dogs should continue as a species in which avian influenza virus may acquire infectivity to mammals through frequent contact with the virus...
April 6, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28385006/intra-abdominal-necrotic-lipoma-diagnosed-as-a-paraprostatic-cyst-by-ct-scan
#9
Hye-Jin Kim, Su-Yeon Kim, Seong-Soo Kim, Soo-Kyung Yun, Jeo-Soon Lee, Sung-Kyun Hong, Wan-Hee Kim, Yong-Baek Kim, Jung-Hee Yoon, Min-Cheol Choi
A dog with a history of diarrhea, dyschezia showed an oval shaped, soft tissue opacity mass was identified in the abdomen on radiographs. CT examination revealed a large fluid-filled structure which displaced the UB, prostate and colon. The mass has continuity with the prostate. Therefore it was tentatively diagnosed as a paraprostatic cyst. Cytologic examination was performed and it was considered as a non-inflammatory cyst. After surgery, histopathologic examination revealed a necrotic, inflamed cystic lipoma...
April 6, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28385005/kcho-1-mecasin-a-novel-herbal-anti-inflammatory-compound-attenuates-oxidative-stress-in-an-animal-model-of-amyotrophic-lateral-sclerosis
#10
Myung Geun Kook, Soon Won Choi, Yoojin Seo, Dong Woung Kim, Bong Keun Song, Ilhong Son, Sungchul Kim, Kyung-Sun Kang
Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease characterized by selective death of motor neurons in the central nervous system. The main cause of the disease is still elusive but several mutations have been associated with the disease process. In particular, mutant SOD1 protein causes oxidative stress by activating glia cells and contributes to the motor neuron degeneration. KCHO-1, a novel herbal combination compound, contains 30% ethanol and the extracts of nine herbs that have been commonly used in traditional medicine to prevent fatigue or inflammation...
April 6, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28385004/molecular-and-genomic-features-of-mycobacterium-bovis-strain-1595-isolated-from-korean-cattle
#11
Narae Kim, Yunho Jang, Jin Kyoung Kim, Soyoon Ryoo, Ka Hee Kwon, Miso Kim, Shin Seok Kang, Hyeon Seop Byeon, Hee Soo Lee, Young-Hee Lim, Jae-Myung Kim
The aim of this study was to investigate the molecular characteristics and conduct a comparative genomic analysis for Mycobacterium bovis strain 1595 isolated from a native Korean cow. Molecular typing showed that M. bovis 1595 has spoligotype SB0140 with mycobacterial interspersed repetitive units-variable number of tandem repeats typing of 4-2-5-3-2-7-5-5-4-3-4-3-4-3, representing the most common type of M. bovis in Korea. The complete genome sequence of strain 1595 was determined using Illumina MiSeq and PacBio single-molecule real-time technology, which showed a genome of 4,351,712 bp in size with a 65...
April 6, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28385003/infection-to-pathogens-with-different-transmission-modes-in-feral-cats-from-urban-and-rural-areas-of-korea
#12
Jusun Hwang, Nicole Gottdenker, Dae-Hyun Oh, Hang Lee, Myung-Sun Chun
In this study, we examine the prevalence of three infectious pathogens with different transmission modes (Bartonella henselae, hemoplasma and Toxoplasma gondii) in feral cats (Felis catus) from two unique habitat types, urban and rural. We determined the infection status of three pathogens in blood samples (total N=117) through molecular or serological diagnostic methods. Overall prevalence of hemoplasma, T. gondii and B. henselae was 47.9%, 50% and 35.7%, respectively. Only seroprevalence of B. henselae was significantly higher in urban cats...
April 6, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28385002/generation-characterization-and-application-in-serodiagnosis-of-recombinant-swine-vesicular-disease-viral-like-particles
#13
Wanhong Xu, Melissa Goolia, Tim Salo, Zhidong Zhang, Ming Yang
Swine vesicular disease virus (SVDV) is a highly contagious disease that causes vesicular disease in pigs. The importance of the disease is due to its indistinguishable clinical signs from foot-and-mouth disease that prevents international trade of swine and related products. SVD-specific antibody detection using the enzyme-linked immunosorbent assay (ELISA) is the most versatile and commonly used method for SVD surveillance and export certification. The inactivated SVD virus is the commonly used antigen in ELISAs...
April 6, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28385001/comparative-kinematic-gait-analysis-in-young-and-old-beagle-dogs
#14
Malin Lorke, Maray Willen, Karin Lucas, Martin Beyerbach, Patrick Wefstaedt, Hugo Murua Escobar, Ingo Nolte
Age-related involution in dogs involves loss of muscle mass and changes in connective tissue and articular cartilage. The aim of this study was to examine whether an age-related influence on joint mobility can be detected in the absence of diseases. Five young (Ø 2.0 years) and five old (Ø 10.4 years) healthy and sound Beagle dogs were measured during locomotion on a treadmill by computer-assisted gait analysis. Angles of the shoulder, elbow, carpal, hip, stifle and tarsal joints were analyzed, including joint angle progression curves, minimum and maximum joint angles and range of motion (ROM)...
April 6, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28385000/potential-biomarkers-as-the-indicator-of-vertical-transmission-of-johne-s-disease-in-a-korean-native-cattle-farm
#15
Hong-Tae Park, Hyun-Eui Park, Yong-Il Cho, Eui-Hyung Kim, Myunghwan Jung, Seung Won Shin, Su-Hyung Lee, Dae-Yong Kim, Han Sang Yoo
Paratuberculosis (PTB) is caused by Mycobacterium avium subsp. paratuberculosis (MAP) which is one of the most widespread and economically important disease in cattle. Calves are raising with natural breast feeding without separation from their mothers after birth in most Korean native cattle farms (Hanwoo breed). Vertical transmission of the disease has been known even though exact infection route of PTB has not been revealed in Korean native cattle farm. Therefore, calves from MAP sero-positive cows were tested by detection of MAP in the feces and tissues, and antibody against MAP...
April 6, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28384999/pathogenesis-of-coxsackievirus-b2-in-mice-characterisation-of-clinical-isolates-of-the-coxsackievirus-b2-from-patients-with-myocarditis-and-aseptic-meningitis-in-korea
#16
JiYoung Hong, BungHak Kang, SangGu Yeo, Youngmee Jee, Jae-Hak Park
Group B coxsackieviruses (CVBs) are a group of common human pathogens causing various clinical symptoms. Although the virology of CVB is well known, there is limited information on viral pathogenesis and the relationship between clinical symptoms and viral phenotype, particularly for CVB type 2 (CVB2). In this study, two CVB2 strains were isolated: CB2/04/279 from a stool of an acute myocarditis patient with heart failure; and CB2/04/243 from an aseptic meningitis patient in 2004 in Korea. A high degree of homology was observed between the CB2/04/279 and CB2/04/243 full genome sequences...
April 6, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/27659717/antibiotic-resistance-patterns-and-genetic-relatedness-of-enterococcus-faecalis-and-enterococcus-faecium-isolated-from-military-working-dogs-in-korea
#17
Kiman Bang, Jae-Uk An, Woohyun Kim, Hee-Jin Dong, Junhyung Kim, Seongbeom Cho
Enterococcus spp. are normally present in the gastrointestinal tracts of animals and humans, but can cause opportunistic infections that can be transmitted to other animals or humans with integrated antibiotic resistance. To investigate if this is a potential risk in military working dogs (MWDs), we analyzed antibiotic resistance patterns and genetic relatedness of Enterococcus spp. isolated from fecal samples of MWDs of four different age groups. Isolation rates of Enterococcus spp., Enterococcus (E.) faecalis, and E...
June 30, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/27586462/efficacy-evaluation-of-commercial-disinfectants-by-using-salmonella-enterica-serovar-typhimurium-as-a-test-organism
#18
Yangho Jang, Kwangjick Lee, Seonjong Yun, Myoungheon Lee, Jaeyoung Song, Byungjoon Chang, Nong-Hoon Choe
The efficacies of six commercial disinfectants were evaluated by using Salmonella enterica serovar Typhimurium under simulated natural conditions such as sub-zero temperature, short disinfecting time, and surface type (uneven or smooth). We used a suspensionmodel test to determine the disinfecting efficacy under varying contact times (1, 5, 10, and 30 min) and temperatures (25°C, 4°C, 0°C, and -10°C). The bactericidal effect according to surface structure was measured by using a carriermodel test at 25°C and -10°C...
June 30, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/27586461/bovine-adenovirus-type-3-virions-cannot-be-rescued-in-vivo-after-full-length-viral-genome-transfection-in-the-absence-of-detectable-polypeptide-ix
#19
Peng Zhang, Qinghong Xue, Jing Ma, Jingjing Ren, Shuili Xia, Lu Zhang, Wenbin Wang, Suresh K Tikoo, Enqi Du
Bovine adenovirus type 3 (BAdV3) is being used in the development of potential vehicles for gene therapy and vectored vaccine. To that end, a more comprehensive description of BAdV3 biology is essential. In this study, we focused on the role of pIX in BAdV3 virion rescue after full-length BAdV3 genome transfection. Initially, pIX deletion or initiation codon mutation abolished the production of progeny virions, which suggested that pIX was essential for the rescue of BAdV3 containing a full-length genome. Moreover, through transfection of a panel of pIX mutant BAdV3 genomes, we observed that the conserved N-terminus and the putative leucine zipper element (PLZP) were essential for virion rescue, whereas the C-terminus following the coiled-coil domain was non-essential...
June 30, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/27515273/effects-of-neural-stem-cells-and-5-fluorocytosine-in-canine-metastatic-lung-tumor
#20
Yawon Hwang, Dongwook Kim, Dongwoo Chang, Byeongwoo Ahn, Yun-Bae Kim, Gonhyung Kim
This is the first case report to describe the tumor regressive effect of systemic human neural stem cell (NSC)/5-fluorocytosine (5-FC) therapy on canine metastatic lung tumor. The therapeutic effects appeared approximately two weeks after 5-FC administration. Thoracic radiographs revealed a reduced number of lung nodules and decreased nodule size. However, there were no significant antitumor effects on primary lesions in abdominal organs. In conclusion, human NSC/5-FC prodrug therapy can secure patient quality of life with the same or more therapeutic effects and fewer side effects than other recommended chemotherapies...
June 30, 2017: Journal of Veterinary Science
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