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Journal of Veterinary Science

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https://www.readbyqxmd.com/read/27659847/bordetella-bronchiseptica-antigen-enhances-the-production-of-mycoplasma-hyopneumoniae-antigen-specific-immunoglobulin-g-in-mice
#1
Seol-Hwa Yim, Tae-Wook Hahn, Hong-Gu Joo
We previously demonstrated that Bordetella bronchiseptica (B. bronchiseptica) antigen (Ag) showed high immunostimulatory effects on mouse bone marrow cells (BMs) while Mycoplasma hyopneumoniae (M. hyopneumoniae) Ag showed low. The focus of this study was if B. bronchiseptica Ag may enhance M. hyopneumoniae Ag-specific immune response, and also the host immune system can recognize both Ags. MTT assay revealed that each or both Ags did not significantly change the cell metabolic activity of BMs. Flow cytometry analysis using carboxyfluorescein succinimidyl ester presented that B...
September 23, 2016: Journal of Veterinary Science
https://www.readbyqxmd.com/read/27030199/transgenesis-for-pig-models
#2
Soo-Young Yum, Ki-Young Yoon, Choong-Il Lee, Byeong-Chun Lee, Goo Jang
Animal models, particularly pigs, have come to play an important role in translational biomedical research. There have been many pig models with genetically modifications via somatic cell nuclear transfer (SCNT). However, because most transgenic pigs have been produced by random integration to date, the necessity for more exact gene-mutated models using recombinase based conditional gene expression like mice has been raised. Currently, advanced genome-editing technologies enable us to generate specific gene-deleted and -inserted pig models...
September 30, 2016: Journal of Veterinary Science
https://www.readbyqxmd.com/read/26726030/aristolochia-manshuriensis-kom-ethyl-acetate-extract-protects-against-high-fat-diet-induced-non-alcoholic-steatohepatitis-by-regulating-kinase-phosphorylation-in-mouse
#3
Dong Hoon Kwak, Ji-Su Kim, Kyu-Tae Chang, Young-Kug Choo
Aristolochia manshuriensis Kom (AMK) is an herb used as a traditional medicine; however, it causes side effects such as nephrotoxicity and carcinogenicity. Nevertheless, AMK can be applied in specific ways medicinally, including via ingestion of low doses for short periods of time. Non-alcoholic steatohepatitis (NASH) induced the hepatocyte injury and inflammation. The protective effects of AMK against NASH are unclear; therefore, in this study, the protective effects of AMK ethyl acetate extract were investigated in a high-fat diet (HFD)-induced NASH model...
September 30, 2016: Journal of Veterinary Science
https://www.readbyqxmd.com/read/26726029/molecular-characterization-and-phylogenetic-analysis-of-pseudorabies-virus-variants-isolated-from-guangdong-province-of-southern-china-during-2013-2014
#4
Jindai Fan, Xiduo Zeng, Guanqun Zhang, Qiwen Wu, Jianqiang Niu, Baoli Sun, Qingmei Xie, Jingyun Ma
Outbreaks of pseudorabies (PR) have occurred in southern China since late 2011, resulting in significant economic impacts on the swine industry. To identify the cause of PR outbreaks, especially among vaccinated pigs, 11 pseudorabies virus (PRV) field strains were isolated from Guangdong province during 2013-2014. Their major viral genes (gE, TK, gI, PK, gD, 11K, and 28K) were analyzed in this study. Insertions or deletions were observed in gD, gE, gI and PK genes compared with other PRV isolates from all over the world...
September 30, 2016: Journal of Veterinary Science
https://www.readbyqxmd.com/read/26726028/immunologic-properties-of-differentiated-and-undifferentiated-mesenchymal-stem-cells-derived-from-umbilical-cord-blood
#5
Hyo-Jong Lee, Kyung-Sun Kang, Sun-Young Kang, Hyung-Sik Kim, Se-Jin Park, Seung-Yong Lee, Kwang-Dong Kim, Hee-Chun Lee, Ji-Kwon Park, Won-Young Paik, Lyon Lee, Seong-Chan Yeon
The expression of immunogenic markers after differentiation of umbilical cord blood (UCB)-derived mesenchymal stem cells (MSC) has been poorly investigated and requires extensive in vitro and in vivo testing for clinical application. The expression of human leukocyte antigen (HLA) classes on UCB-derived MSC was tested by Fluorescence-activated cell sorting analysis and immunocytochemical staining. The undifferentiated MSC were moderately positive for HLA-ABC, but almost completely negative for HLA-DR. The MSC differentiated to chondrocytes expressed neither HLA-ABC nor HLA-DR...
September 30, 2016: Journal of Veterinary Science
https://www.readbyqxmd.com/read/26726027/uracil-dna-glycosylase-treated-reverse-transcription-loop-mediated-isothermal-amplification-for-rapid-detection-of-avian-influenza-virus-preventing-carry-over-contamination
#6
Eun-Mi Kim, Hyo-Sung Jeon, Ji-Jung Kim, Yeun-Kyung Shin, Youn-Jeong Lee, Sang-Geon Yeo, Choi-Kyu Park
Here, we describe a uracil-DNA glycosylase (UNG)-treated reverse transcription loop-mediated isothermal amplification (uRT-LAMP) for the visual detection of all subtypes of avian influenza A virus (AIV). The uRT-LAMP assay can prevent unwanted amplification by carryover contamination of the previously amplified DNA, although the detection limit of the uRT-LAMP assay is 10-fold lower than that of the RT-LAMP without a UNG treatment. To the best of our knowledge, this is the first successful application of deoxyuridine triphosphate/UNG strategy in RT-LAMP for AIV detection, and the assay can be applied for the rapid, and reliable diagnosis of AIVs, even in contaminated samples...
September 30, 2016: Journal of Veterinary Science
https://www.readbyqxmd.com/read/26726026/mycobacterium-bovis-infection-in-a-wild-sow-sus-scrofa-first-case-in-korea
#7
Bok Kyung Ku, Bo-Young Jeon, Jae Myung Kim, Young-Boo Jang, Yunho Jang, So Yoon Yu, Jiro Kim, Oun Kyung Moon, Suk Chan Jung, Min Kwon Lee, Tae Nam Jeong
Mycobacterium (M.) bovis causes tuberculosis and has a broad host range, including humans, livestock, and wild animals. M. bovis infection of wild boar has been reported in several European countries. We report here the first case of M. bovis infection in a domesticated wild sow in Korea. Granulomatous and necrotizing lesions with small numbers of acid-fast bacilli were observed in nodules of the lung of wild sow. Furthermore, the M. bovis isolate from the wild sow had spoligotype SB0140 and a novel MIRU-VNTR allelic profile, which is not found in cattle and deer in Korea...
September 30, 2016: Journal of Veterinary Science
https://www.readbyqxmd.com/read/26726025/hematologic-and-serum-biochemical-reference-intervals-of-the-oriental-white-stork-ciconia-boyciana-and-the-application-of-an-automatic-hematologic-analyzer
#8
Jae-Ik Han, Hye-Jin Jang, Ki-Jeong Na
This study was conducted to establish accurate baseline values of clinical laboratory data with regard to age-related changes in the Oriental white stork (Ciconia boyciana). In addition, the availability of an automated hematological cell counter was evaluated. A total of 94 clinically normal storks, including 64 young storks (<1 year old; 30 male and 34 female) and 30 adults (> 1 year old; 17 male and 13 female) were included. Hematological assays were performed using manual and automated cell counters and serum biochemistry profiles were examined using an automated analyzer...
September 30, 2016: Journal of Veterinary Science
https://www.readbyqxmd.com/read/26726024/establishment-of-a-canine-mammary-gland-tumor-cell-line-and-characterization-of-its-mirna-expression
#9
Tomohiro Osaki, Yuji Sunden, Akihiko Sugiyama, Kazuo Azuma, Yusuke Murahata, Takeshi Tsuka, Norihiko Ito, Tomohiro Imagawa, Yoshiharu Okamoto
Canine mammary gland tumors (CMGTs), which are the most common neoplasms in sexually intact female dogs, have been suggested as a model for studying human breast cancer because of several similarities, including relative age of onset, risk factors, incidence, histological and molecular features, biological behavior, metastatic pattern, and responses to therapy. In the present study, we established a new cell line, the SNP cell line, from a CMGT. A tumor formed in each NOD.CB17-Prkdc(scid)/J mouse at the site of subcutaneous SNP cell injection...
September 30, 2016: Journal of Veterinary Science
https://www.readbyqxmd.com/read/26726023/hyperammonemic-hepatic-encephalopathy-management-through-l-ornithin-l-aspartate-administration-in-dogs
#10
Jin-Ok Ahn, Qiang Li, Young-Heun Lee, Sei-Myoung Han, Cheol-Yong Hwang, Hwa-Young Youn, Jin-Young Chung
Seventeen dogs were treated with L-ornithin-L-aspartate (LOLA; experimental group). Three dogs were treated with lactulose recognized therapy (control group). Following LOLA administration, 15 dogs experienced a significant decrease in ammonia level (p < 0.05) and showed clinical signs of improvement. However, there were no clinical signs of improvement in two dogs, even though the ammonia level decreased. Conversely, the clinical signs of the control group also improved and the ammonia level decreased, although these changes were not significant (p > 0...
September 30, 2016: Journal of Veterinary Science
https://www.readbyqxmd.com/read/26726022/matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-based-identification-of-edwardsiella-ictaluri-isolated-from-vietnamese-striped-catfish-pangasius-hypothalamus
#11
Truong Quynh Nhu, Seong Bin Park, Si Won Kim, Jung Seok Lee, Se Pyeong Im, Jassy Mary S Lazarte, Jong Pyo Seo, Woo-Jai Lee, Jae Sung Kim, Tae Sung Jung
Edwardsiella (E.) ictaluri is a major bacterial pathogen that affects commercially farmed striped catfish (Pangasius hypothalamus) in Vietnam. In a previous study, 19 strains of E. ictaluri collected from striped catfish were biochemically identified with an API-20E system. Here, the same 19 strains were used to assess the ability of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS; applied using a MALDI Biotyper) to conduct rapid, easy and accurate identification of E...
September 30, 2016: Journal of Veterinary Science
https://www.readbyqxmd.com/read/26726021/seroprevalence-and-associated-risk-factors-of-pseudorabies-in-shandong-province-of-china
#12
Dongfang Hu, Lin Lv, Zhendong Zhang, Yihong Xiao, Sidang Liu
A cross-sectional serological study was conducted in Shandong province of China to determine the seroprevalence and risk factors associated with seropositivity due to pseudorabies virus (PRV) infection in small- and medium-sized farrow-to-finish herds following outbreaks of variant PRV strains. A total of 6,035 blood samples from 224 randomly selected herds were screened. The results showed that 25.0% of the herds and 56.7% of the serum samples were seropositive for field strains of PRV. Herds consisting of 50-100 breeding sows had higher herd seroprevalence and serum sample seroprevalence than larger herds...
September 30, 2016: Journal of Veterinary Science
https://www.readbyqxmd.com/read/26726020/establishment-of-hydrochloric-acid-lipopolysaccharide-induced-pelvic-inflammatory-disease-model
#13
Yeonsu Oh, Jaehun Lee, Hyeon-Cheol Kim, Tae-Wook Hahn, Byung-Il Yoon, Jeong-Hee Han, Yong-Soo Kwon, Joung Jun Park, Deog-Bon Koo, Ki-Jong Rhee, Bae Dong Jung
Pelvic inflammatory disease (PID), which is one of the most problematic complications experienced by women with sexually transmitted diseases, frequently causes secondary infections after reproductive abnormalities in veterinary animals. Although the uterus is self-protective, it becomes fragile during periods or pregnancy. To investigate PID, bacteria or lipopolysaccharide (LPS) extracted from gram negative bacteria has been used to induce the disease in several animal models. However, when LPS is applied to the peritoneum, it often causes systemic sepsis leading to death and the PID was not consistently demonstrated...
September 30, 2016: Journal of Veterinary Science
https://www.readbyqxmd.com/read/26726019/tick-borne-rickettsial-pathogens-in-questing-ticks-removed-from-humans-and-animals-in-mexico
#14
Carolina G Sosa-Gutierrez, Margarita Vargas-Sandoval, Javier Torres, Guadalupe Gordillo-Pérez
Tick-borne rickettsial diseases (TBRD) are commonly encountered in medical and veterinary clinical settings. The control of these diseases is difficult, requiring disruption of a complex transmission chain involving a vertebrate host and ticks. The geographical distribution of the diseases is related to distribution of the vector, which is an indicator of risk for the population. A total of 1107 were collected by tick drag from forests, ecotourism parks and hosts at 101 sites in 22 of the 32 states of Mexico...
September 30, 2016: Journal of Veterinary Science
https://www.readbyqxmd.com/read/26726018/the-current-epidemiological-status-of-infectious-coryza-and-efficacy-of-poulshot-coryza-in-specific-pathogen-free-chickens
#15
Moo-Sung Han, Jong-Nyeo Kim, Eun-Ok Jeon, Hae-Rim Lee, Bon-Sang Koo, Kyeong-Cheol Min, Seung-Baek Lee, Yeon-Ji Bae, Jong-Suk Mo, Sun-Hyung Cho, Hye-Sun Jang, In-Pil Mo
Infectious coryza (IC) is an infectious disease caused by Avibacterium (Av.) paragallinarum. IC is known to cause economic losses in the poultry industry via decreased egg production in layers. Between 2012 and 2013, Av. paragallinarum was isolated from seven chicken farms by Chungbuk National University. We identified Av. paragallinarum, the causative pathogen of IC by polymerase chain reaction (PCR) and serovar serotype A, by multiplex PCR. Antibiotic sensitivity tests indicated that a few field-isolated strains showed susceptibility to erythromycin, gentamicin, lincomycin, neomycin, oxytetracycline, spectinomycin, and tylosin...
September 30, 2016: Journal of Veterinary Science
https://www.readbyqxmd.com/read/26726017/inhibitory-effect-of-red-ginseng-acidic-polysaccharide-from-korean-red-ginseng-on-phagocytic-activity-and-intracellular-replication-of-brucella-abortus-in-raw-264-7-cells
#16
Alisha Wehdnesday Bernardo Reyes, Hannah Leah Tadeja Simborio, Huynh Tan Hop, Lauren Togonon Arayan, Won Gi Min, Hu Jang Lee, Man Hee Rhee, Hong Hee Chang, Suk Kim
Korean red ginseng (KRG) has long been used in traditional Korean and Oriental medicine. However, the anti-bacterial mechanism and therapeutic efficiency of KGR for intracellular Brucella infection are still unclear. In this study, the bactericidal activity of Korean red ginseng acidic polysaccharide (RGAP) on Brucella (B.) abortus and its cytotoxic effects on RAW 264.7 cells were evaluated. In addition, B. abortus internalization and intracellular replication in macrophages were investigated after RGAP treatment...
September 30, 2016: Journal of Veterinary Science
https://www.readbyqxmd.com/read/26645343/hydration-status-affects-osteopontin-expression-in-the-rat-kidney
#17
Su-Youn Lee, Sae-Jin Lee, Hong-Lin Piao, Suk-Young Yang, I David Weiner, Jin Kim, Ki-Hwan Han
Osteopontin (OPN) is a secretory protein that plays an important role in urinary stone formation. Hydration status is associated with the development of urolithiasis. This study was conducted to examine the effects of dehydration and hydration on OPN expression in the rat kidney. Animals were divided into three groups, control, dehydrated, and hydrated. Kidney tissues were processed for light and electron microscope immunocytochemistry, in situhybridization, and immunoblot analysis. Dehydration induced a significant increase in OPN protein expression, whereas increased fluid intake induced a decrease in protein expression...
September 30, 2016: Journal of Veterinary Science
https://www.readbyqxmd.com/read/26645338/diagnostic-imaging-features-of-normal-anal-sacs-in-dogs-and-cats
#18
Yechan Jung, Eunseok Jeong, Sangjun Park, Jimo Jeong, Ul Soo Choi, Min-Su Kim, Namsoo Kim, Kichang Lee
This study was conducted to provide normal reference features for canine and feline anal sacs using ultrasound, low-field magnetic resonance imaging (MRI) and radiograph contrast as diagnostic imaging tools. A total of ten clinically normal beagle dogs and eight clinically normally cats were included. General radiography with contrast, ultrasonography and low-field MRI scans were performed. The visualization of anal sacs, which are located at distinct sites in dogs and cats, is possible with a contrast study on radiography...
September 30, 2016: Journal of Veterinary Science
https://www.readbyqxmd.com/read/26645337/population-genetic-study-of-10-short-tandem-repeat-loci-from-600-domestic-dogs-in-korea
#19
Seo Hyun Moon, Yoon-Jeong Jang, Myun Soo Han, Myung-Haing Cho
Dogs have long shared close relationships with many humans. Due to the large number of dogs in human populations, they are often involved in crimes. Occasionally, canine biological evidence such as saliva, bloodstains and hairs can be found at crime scenes. Accordingly, canine DNA can be used as forensic evidence. The use of short tandem repeat (STR) loci from biological evidence is valuable for forensic investigations. In Korea, canine STR profiling-related crimes are being successfully analyzed, leading to diverse crimes such as animal cruelty, dog-attacks, murder, robbery, and missing and abandoned dogs being solved...
September 30, 2016: Journal of Veterinary Science
https://www.readbyqxmd.com/read/26645335/deep-anterior-lamellar-keratoplasty-of-dog-eyes-using-the-big-bubble-technique
#20
Soohyun Kim, Ji Yoon Kwak, Manbok Jeong, Kangmoon Seo
This study was conducted to establish the feasibility of corneal transplantation using the big-bubble technique (BBT) to perform deep anterior lamellar keratoplasty (DALK) in three dogs. After the cornea was trephined 750 µm, 4 mL of air was injected, and the blanched stroma was removed to expose Descemet's membrane (DM). The donor corneal button, which was gently stripped off the DM, was sutured onto the bare DM of the recipient cornea. The dogs received topical antibiotics every 6 h for 7 days and 2% cyclosporine ointment every 12 h for 1 month...
September 30, 2016: Journal of Veterinary Science
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