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Journal of Veterinary Science

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https://www.readbyqxmd.com/read/28385014/mechanisms-of-quinolone-resistance-in-escherichia-coli-isolated-from-companion-animals-pet-owners-and-non-pet-owners
#1
Yeon Soo Chung, Yoon Sung Hu, Sook Shin, Suk Kyung Lim, Soo Jin Yang, Yong Ho Park, Kun Taek Park
The present study investigated the prevalence and the mechanisms of fluoroquinolone (FQ)/quinolone (Q) resistance in Escherichia coli isolates from companion animals, pet-owners, and non-pet owners. A total of 63 E. coli isolates were collected from 104 anal swab samples; 27 nalidixic acid (NA)-resistant isolates were identified. Of these, 10 showed ciprofloxacin (CIP) resistance. Plasmid-mediated Q resistance gene was detected in an isolate. Increased efflux pump activity, as measured by organic solvent tolerance assay, was detected in 18 isolates (66...
April 6, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28385013/quantitative-evaluation-of-renal-parenchymal-perfusion-using-contrast-enhanced-ultrasonography-in-renal-ischemia-reperfusion-injury-in-dogs
#2
Gahyun Lee, Sunghoon Jeon, Sang-Kwon Lee, Byunggyu Cheon, Sohyeon Moon, Jun-Gyu Park, Kyoung-Oh Cho, Jihye Choi
This study evaluated whether renal perfusion changes can be noninvasively estimated using contrast-enhanced ultrasonography (CEUS) in renal ischemia-reperfusion injury and investigated the correlation between renal perfusion measured by CEUS and necrosis and apoptosis of renal tubular epithelial cell. In six dogs with experimentally induced renal ischemia-reperfusion injury, changes in time to peak intensity, peak intensity and area under the curve were measured on CEUS. Peak intensity and area under the curve of the renal cortex began to decrease by about 20% compared to baseline from day 1, and reached its lowest level, about 50% to baseline, on day 4...
April 6, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28385012/eosinophilic-encephalomyelitis-in-horses-caused-by-protostrongylid-parasites
#3
Eun-Jung Bak, Young-Hwa Jean, Gye-Hyeong Woo
Four thoroughbred horses showing lameness, ataxia, circling, depression, recumbency, and seizures, were submitted. The horses had grossly weak to dark red appearances and foci in the central nervous system. Multifocal to coalescing eosinophilic necrotizing encephalomyelitis was observed histologically in the CNS as well as intact or degenerated nematodes. Nematodes had polymyarian-coelomyarian musculature, a smooth thin cuticle, and intestines lined by multinucleated cells with microvilli. These traits suggested the nematodes belonged to the family Protostrongylidae, which includes P...
April 6, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28385011/pharmacokinetics-of-tilmicosin-in-healthy-pigs-and-in-pigs-experimentally-infected-with-haemophilus-parasuis
#4
Ling Zhang, Li Zhao, Yonghong Liu, Junfeng Liu, Xianqiang Li
A comparative in vivo pharmacokinetic (PK) study of tilmicosin (TIL) was conducted in 6 crossbred healthy pigs and 6 crossbred pigs infected with Haemophilus parasuis (H. parasuis) following oral administration of a single dose of 40 mg/kg. The infected model was established by intranasal inoculation and confirmed by clinical signs, blood biochemical and microscopic examination. TIL concentrations in plasma were determined by a validated high-performance liquid chromatography (HPLC) method with ultra violet detection at a wavelength of 285 nm and PK parameters were calculated by using the Winnonlin software...
April 6, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28385010/coactosin-like-protein-1-inhibits-the-neuronal-migration-during-mouse-corticogenesis
#5
Guohong Li, Yupeng Yin, Jiong Chen, Yanle Fan, Juhong Ma, Yingxue Huang, Chen Chen, Pengxiu Dai, Shulin Chen, Shanting Zhao
Coactosin-like protein-1 (Cotl1), a member of the actin-depolymerizing factor (ADF)/cofilin family, was first purified from the soluble fraction of Dictyostelium discoideum cells. Neuronal migration requires cytoskeletal remodeling and actin regulation. Although Cotl1 strongly binds to F-actin, the role of Cotl1 in neuronal migration remains unknown. Here, we revealed that Cotl1 overexpression impaired the migration of both early- and late-born neurons during mouse corticogenesis. Moreover, Cotl1 overexpression delayed, rather than blocked, neuronal migration in late-born neurons...
April 6, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28385009/inhibitory-effects-of-resveratrol-on-hepatitis-b-virus-x-protein-hbx-induced-hepatocellular-carcinoma-hcc
#6
Seungmo Park, Jihae Lim, Jong Rhan Kim, Seongbeom Cho
Liver cancer occurs very frequently worldwide and hepatocellular carcinoma (HCC) accounts for more than 80% of total primary liver cancer cases. Here, the anticarcinogenic effects of resveratrol against hepatitis B virus (HBV)-induced HCC were investigated using HBV X-protein-overexpressing Huh7 (Huh7-HBx) human hepatoma cells. MTT assay showed that resveratrol decreased cell viability. Fluorescence-activated cell sorter analysis showed that it induced G1 cell cycle arrest without increasing the sub-G1 phase cell population...
April 6, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28385008/use-of-stem-cell-sheets-expressing-bone-morphogenetic-protein-7-in-the-management-of-a-nonunion-radial-fracture-in-a-toy-poodle
#7
Jaeyong Song, Yongsun Kim, Oh-Kyeong Kweon, Byung-Jae Kang
A 12-year-old castrated Toy Poodle was referred to Kangwon National University Animal Hospital with an oligotrophic nonunion fracture in the left distal 1/3 of the radius and an intact ulna. After fixation using a locking plate and screws, adipose-derived mesenchymal stem cell sheets expressing bone morphogenetic protein 7 (BMP-7) were transplanted to the fracture site to enhance the healing activity. The fracture healed at 9 weeks after surgery. In the present case, the mesenchymal stem cell sheets expressing BMP-7 promoted bone regeneration and healing in a nonunion fracture...
April 6, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28385007/experimental-infection-of-dogs-with-highly-pathogenic-avian-influenza-virus-h5n8
#8
Seong-Su Yuk, Dong-Hun Lee, Jae-Keun Park, Eredene-Ochir To, Jung-Hoon Kwon, Jin-Yong Noh, Chang-Seon Song
During the highly pathogenic avian influenza (HPAI) H5N8 outbreak in South Korea, a dog reared in layer farm contaminated by the virus was reported seropositive for HPAI H5N8. In order to investigate the possibility of the adaptation and transmission of HPAI H5N8 to dogs, we inoculated the H5N8 to dogs in experimental condition. Weak viral genes in nasal swabs and seroconversions were detected even in contact dogs. Although the H5N8 virus doesn't seem to adapt to canine species fully, these results suggest that surveillance on farm dogs should continue as a species in which avian influenza virus may acquire infectivity to mammals through frequent contact with the virus...
April 6, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28385006/intra-abdominal-necrotic-lipoma-diagnosed-as-a-paraprostatic-cyst-by-ct-scan
#9
Hye-Jin Kim, Su-Yeon Kim, Seong-Soo Kim, Soo-Kyung Yun, Jeo-Soon Lee, Sung-Kyun Hong, Wan-Hee Kim, Yong-Baek Kim, Jung-Hee Yoon, Min-Cheol Choi
A dog with a history of diarrhea, dyschezia showed an oval shaped, soft tissue opacity mass was identified in the abdomen on radiographs. CT examination revealed a large fluid-filled structure which displaced the UB, prostate and colon. The mass has continuity with the prostate. Therefore it was tentatively diagnosed as a paraprostatic cyst. Cytologic examination was performed and it was considered as a non-inflammatory cyst. After surgery, histopathologic examination revealed a necrotic, inflamed cystic lipoma...
April 6, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28385005/kcho-1-mecasin-a-novel-herbal-anti-inflammatory-compound-attenuates-oxidative-stress-in-an-animal-model-of-amyotrophic-lateral-sclerosis
#10
Myung Geun Kook, Soon Won Choi, Yoojin Seo, Dong Woung Kim, Bong Keun Song, Ilhong Son, Sungchul Kim, Kyung-Sun Kang
Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease characterized by selective death of motor neurons in the central nervous system. The main cause of the disease is still elusive but several mutations have been associated with the disease process. In particular, mutant SOD1 protein causes oxidative stress by activating glia cells and contributes to the motor neuron degeneration. KCHO-1, a novel herbal combination compound, contains 30% ethanol and the extracts of nine herbs that have been commonly used in traditional medicine to prevent fatigue or inflammation...
April 6, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28385004/molecular-and-genomic-features-of-mycobacterium-bovis-strain-1595-isolated-from-korean-cattle
#11
Narae Kim, Yunho Jang, Jin Kyoung Kim, Soyoon Ryoo, Ka Hee Kwon, Miso Kim, Shin Seok Kang, Hyeon Seop Byeon, Hee Soo Lee, Young-Hee Lim, Jae-Myung Kim
The aim of this study was to investigate the molecular characteristics and conduct a comparative genomic analysis for Mycobacterium bovis strain 1595 isolated from a native Korean cow. Molecular typing showed that M. bovis 1595 has spoligotype SB0140 with mycobacterial interspersed repetitive units-variable number of tandem repeats typing of 4-2-5-3-2-7-5-5-4-3-4-3-4-3, representing the most common type of M. bovis in Korea. The complete genome sequence of strain 1595 was determined using Illumina MiSeq and PacBio single-molecule real-time technology, which showed a genome of 4,351,712 bp in size with a 65...
April 6, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28385003/infection-to-pathogens-with-different-transmission-modes-in-feral-cats-from-urban-and-rural-areas-of-korea
#12
Jusun Hwang, Nicole Gottdenker, Dae-Hyun Oh, Hang Lee, Myung-Sun Chun
In this study, we examine the prevalence of three infectious pathogens with different transmission modes (Bartonella henselae, hemoplasma and Toxoplasma gondii) in feral cats (Felis catus) from two unique habitat types, urban and rural. We determined the infection status of three pathogens in blood samples (total N=117) through molecular or serological diagnostic methods. Overall prevalence of hemoplasma, T. gondii and B. henselae was 47.9%, 50% and 35.7%, respectively. Only seroprevalence of B. henselae was significantly higher in urban cats...
April 6, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28385002/generation-characterization-and-application-in-serodiagnosis-of-recombinant-swine-vesicular-disease-viral-like-particles
#13
Wanhong Xu, Melissa Goolia, Tim Salo, Zhidong Zhang, Ming Yang
Swine vesicular disease virus (SVDV) is a highly contagious disease that causes vesicular disease in pigs. The importance of the disease is due to its indistinguishable clinical signs from foot-and-mouth disease that prevents international trade of swine and related products. SVD-specific antibody detection using the enzyme-linked immunosorbent assay (ELISA) is the most versatile and commonly used method for SVD surveillance and export certification. The inactivated SVD virus is the commonly used antigen in ELISAs...
April 6, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28385001/comparative-kinematic-gait-analysis-in-young-and-old-beagle-dogs
#14
Malin Lorke, Maray Willen, Karin Lucas, Martin Beyerbach, Patrick Wefstaedt, Hugo Murua Escobar, Ingo Nolte
Age-related involution in dogs involves loss of muscle mass and changes in connective tissue and articular cartilage. The aim of this study was to examine whether an age-related influence on joint mobility can be detected in the absence of diseases. Five young (Ø 2.0 years) and five old (Ø 10.4 years) healthy and sound Beagle dogs were measured during locomotion on a treadmill by computer-assisted gait analysis. Angles of the shoulder, elbow, carpal, hip, stifle and tarsal joints were analyzed, including joint angle progression curves, minimum and maximum joint angles and range of motion (ROM)...
April 6, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28385000/potential-biomarkers-as-the-indicator-of-vertical-transmission-of-johne-s-disease-in-a-korean-native-cattle-farm
#15
Hong-Tae Park, Hyun-Eui Park, Yong-Il Cho, Eui-Hyung Kim, Myunghwan Jung, Seung Won Shin, Su-Hyung Lee, Dae-Yong Kim, Han Sang Yoo
Paratuberculosis (PTB) is caused by Mycobacterium avium subsp. paratuberculosis (MAP) which is one of the most widespread and economically important disease in cattle. Calves are raising with natural breast feeding without separation from their mothers after birth in most Korean native cattle farms (Hanwoo breed). Vertical transmission of the disease has been known even though exact infection route of PTB has not been revealed in Korean native cattle farm. Therefore, calves from MAP sero-positive cows were tested by detection of MAP in the feces and tissues, and antibody against MAP...
April 6, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28384999/pathogenesis-of-coxsackievirus-b2-in-mice-characterisation-of-clinical-isolates-of-the-coxsackievirus-b2-from-patients-with-myocarditis-and-aseptic-meningitis-in-korea
#16
JiYoung Hong, BungHak Kang, SangGu Yeo, Youngmee Jee, Jae-Hak Park
Group B coxsackieviruses (CVBs) are a group of common human pathogens causing various clinical symptoms. Although the virology of CVB is well known, there is limited information on viral pathogenesis and the relationship between clinical symptoms and viral phenotype, particularly for CVB type 2 (CVB2). In this study, two CVB2 strains were isolated: CB2/04/279 from a stool of an acute myocarditis patient with heart failure; and CB2/04/243 from an aseptic meningitis patient in 2004 in Korea. A high degree of homology was observed between the CB2/04/279 and CB2/04/243 full genome sequences...
April 6, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28316230/phylogeographical-characterization-of-h5n8-viruses-isolated-from-poultry-and-wild-birds-during-2014-2016-in-south-korea
#17
Byung-Min Song, Eun-Kyoung Lee, Yu-Na Lee, Gyeong-Beom Heo, Hee-Soo Lee, Youn-Jeong Lee
During 2014–2016 HPAI outbreak in South Korea, H5N8 viruses have been mostly isolated in western areas of the country, which provide wintering habitats for wild birds and have a high density of poultry. Analysis of a total of 101 Korean isolates revealed that primitive H5N8 viruses (C0 group) have evolved into multiple genetic subgroups appearing from various epidemiological sources, namely, the viruses circulating in poultry farms (C1 and C5) and those reintroduced by migratory birds in late 2014 (C2 and C4)...
March 30, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28057903/reactive-oxygen-species-mediated-unfolded-protein-response-pathways-in-preimplantation-embryos
#18
REVIEW
Ihsan Ali, Syed Zahid Ali Shah, Yi Jin, Zhong-Shu Li, Obaid Ullah, Nan-Zhu Fang
Excessive production of reactive oxygen species (ROS) and endoplasmic reticulum (ER) stress-mediated responses are critical to embryonic development in the challenging in vitro environment. ROS production increases during early embryonic development with the increase in protein requirements for cell survival and growth. The ER is a multifunctional cellular organelle responsible for protein folding, modification, and cellular homeostasis. ER stress is activated by a variety of factors including ROS. Such stress leads to activation of the adaptive unfolded protein response (UPR), which restores homeostasis...
March 30, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/27515264/pulsed-wave-doppler-ultrasonographic-evaluation-of-hepatic-vein-in-dogs-with-tricuspid-regurgitation
#19
Jaehwan Kim, Soyoung Kim, Kidong Eom
This study was performed to identify the relationships between hepatic vein (HV) measurements, including flow velocity and waveform, using pulsed-wave (PW) Doppler ultrasonography, and the severity of tricuspid regurgitation (TR) in dogs. The study included 22 dogs with TR and 7 healthy dogs. The TR group was subdivided into 3 groups according to TR jet profile obtained by echocardiography. The hepatic venous waveform was obtained and classified into 3 types. A variety of HV measurements, including the maximal velocities of the atrial systolic, systolic (S), end ventricular systolic, and diastolic (D) waves and the ratio of the S- and D- wave velocities (S/D ratio), were acquired...
March 30, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/27456777/probiotic-properties-and-adsorption-of-enterococcus-faecalis-psct3-7-to-vermiculite
#20
Jin-Yoon Kim, Elias Gebru Awji, Na-Hye Park, Ji-Yong Park, Jong-Choon Kim, Sam-Pin Lee, Joo-Won Suh, Seung-Chun Park
The probiotic properties of Enterococcus (E.) faecalis PSCT3-7, a new strain isolated from the intestines of pigs fed dietary fiber containing 50% sawdust, were investigated. E. faecalis PSCT3-7 tolerated a pH range of 3 to 8 and 0.3% bile salts, and it inhibited the growth of Salmonella Typhimurium in a concentration-dependent manner. In addition, E. faecalis showed resistance to several antibacterial agents. Vermiculite, a nutrient and microbial carrier, increased the bile tolerance of the strain. Scanning electron microscope images revealed good adsorption of E...
March 30, 2017: Journal of Veterinary Science
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