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Journal of Veterinary Science

Rachel Flores Madera, Lihua Wang, Wenjie Gong, Yulia Burakova, Sterling Buist, Jerome Nietfeld, Jamie Henningson, Ada G Cino Ozuna, Changchun Tu, Jishu Shi
The highly contagious classical swine fever (CSF) remains a major trade and health problem in the pig industry, causing large economic losses worldwide. Modified live vaccines (MLV), commonly derived from the attenuated CSF virus (CSFV) C-strain, have been routinely used to control the disease in CSF-endemic countries. However, to completely eradicate the disease, a potent, safe and non-infectious CSF vaccine should be easily accessible and available. This study aims to develop a cost-effective, non infectious CSF subunit vaccine that can elicit rapid and long lasting immunity...
March 6, 2018: Journal of Veterinary Science
Takeshi Tsuka, Yoshiharu Okamoto, Naoki Yamamoto, Keiji Hayashi, Takehito Morita, Yuji Sunden, Yusuke Murahata, Kazuo Azuma, Tomohiro Osaki, Norihiko Ito, Tomohiro Imagawa
A 2-month-old female Holstein calf and a 5-month-old female Japanese black calf presented with a gingival vascular hamartoma located in the interdental space between the second and third mandibular incisors in the right and left mandibles, respectively. Osteolytic changes appeared within the mandibular bones adjacent to the masses on radiographic or computed tomographic images. The masses were removed along with affected mandibular bone by unilateral rostral mandibulectomy. Two cases exhibited a normal appetite and grew normally, with no cosmetic changes and recurrences...
March 6, 2018: Journal of Veterinary Science
Ji Eun Yu, In-Ohk Ouh, Hyeonjeong Kang, Hye-Young Lee, Kwang-Myun Cheong, In-Soo Cho, Sang-Ho Cha
Porcine reproductive and respiratory syndrome (PRRS) has been proposed to be one of the most important infectious diseases causing serious economic loss in swine industry worldwide. Due to its increasing genetic diversity, a rapid and accurate diagnosis is critical in PRRS control. The immunochromatographic strip test (ICST) has been recognized as a type of immunoassay with speed and convenience. In this study, the on-site diagnostic method with immunochromatographic assay was developed for detection of PRRSV-specific antibodies based on colloidal gold nanoparticles-labeled dual-type nucleocapsid (N) proteins encoded by open reading frame (ORF) 7, and evaluated using 991 field samples from pig farms and 66 serum samples from experimentally PRRSV-inoculated pigs...
March 6, 2018: Journal of Veterinary Science
Babak Faramarzi, An Nguyen, Fanglong Dong
Appropriate hoof preparation and symmetry are linked to the wellbeing of the horse. Previous studies have shown the efficacy of the pressure plates (PP) in delivering objective biomechanical analysis. We aimed to assess the effect of hoof trimming on hoof biomechanics using a PP. Nine clinically sound Arabian horses were walked across the PP while foot strike was recorded by a digital camera. Kinetic and kinematic parameters were recorded before and after trimming. P values ≤ 0.05 were considered significant...
February 27, 2018: Journal of Veterinary Science
Arafat Khalphallah, Nasr-Eldin M Aref, Ashraf M Abu-Seida, Enas Elmeligy, Sara A Bayoumi, Al-Lethie A Al-Lethie, Doaa Salman
This study describes the ultrasonographic findings of five hepatobiliary diseases in buffaloes. Fifty buffaloes including; 20 clinically normal and 30 hepatobiliary diseased buffaloes were enrolled in this study. Complete clinical, radiographic and ultrasonographic examinations and laboratory analyses were conducted. RESULTS: Focal parenchymal lesions including liver abscess (N=12) and hepatic cyst (N=6), diffuse parenchymal lesion (hepatobiliary cirrhosis, N=5) and obstruction of hepatobiliary passages including cholestasis (N=4) and hepatocholelithiasis (N=3) were successfully imaged by ultrasound...
February 27, 2018: Journal of Veterinary Science
Chan Jin Park, Heehyen Kim, Jooyoung Jin, Radwa Barakat, Po-Ching Lin, Jeong Moon Choi, CheMyong Jay Ko
17β-estradiol is synthesized primarily in the gonads of both sexes and regulates the development and function of reproductive organs. Lately, we discovered that intestinal lymphocyte homeostasis is regulated by estradiol synthesized de novo in the endothelial cells of high endothelial venule of mesenteric lymph node and Peyer's patches in mice. This finding prompted us to hypothesize that the high endothelial venule of intestinal lymphoid tissues is the site of estradiol synthesis across the species. In this study, we determined if estradiol is synthesized in the porcine intestinal lymphoid tissues using adolescent piglets...
February 27, 2018: Journal of Veterinary Science
Francesca Del Chicca, Andrea Schwarz, Dieter Meier, Paula Grest, Annette Liesegang, Patrick Robert Kircher
Objective of the present study is to describe two non-invasive methods of fat quantification in the normal canine liver using magnetic resonance imaging and spectroscopy. Eleven adult beagle dogs were anesthetized and underwent magnetic resonance examination of the cranial abdomen including morphologic imaging, a modified Dixon (mDixon) dual gradient echo sequence and proton magnetic resonance spectroscopy (1 H MRS). In addition, ultrasonographic examination of the liver, fine needle aspirates and liver biopsies were performed, and hepatic triglyceride content was assayed...
February 27, 2018: Journal of Veterinary Science
Seung-Chul Lee, Sunhee Lee, Gun-Woo Yoo, Hwan-Won Choi, Yun-Hee Noh, Chang Eon Park, Jae-Ho Shin, In-Joong Yoon, Shien-Young Kang, Changhee Lee
Porcine reproductive and respiratory syndrome virus (PRRSV) is a globally ubiquitous swine viral pathogen that causes major economic losses worldwide. We previously reported an over-attenuated phenotype of a cell-adapted PRRSV strain CA-2-P100 in vivo . In the present study, CA-2-P100 was serially propagated in cultured porcine alveolar macrophage (PAM) cells for up to 20 passages to obtain the derivative strain CA-2-MP120. Animal inoculation studies revealed that both CA-2-P100 and CA-2-MP120 had decreased virulence, eliciting similar weight gain, body temperatures, and histopathologic lesions as in the negative control group...
February 27, 2018: Journal of Veterinary Science
Ann Kristin Barton, Thorben Schulze, Marcus G Doherr, Heidrun Gehlen
Bronchoalveolar lavage (BAL) and thoracic radiography are routinely performed diagnostic procedures. We hypothesized that the BAL increases the interstitial opacity of caudoventral and caudodorsal thoracic radiographs. 53 horses, including 12 clinic owned and 41 horses of a referral-hospital population, were classified as healthy controls (n=12), severe (RAO, n=12) or mild-to-moderate equine asthma (IAD, n=21) according to the results of a clinical scoring system. 4 blinded and randomized thoracic radiographs of each horse were scored by two observers, who were also asked to identify the image as obtained before or after the BAL procedure...
February 27, 2018: Journal of Veterinary Science
Jung-Whan Chon, Kun-Ho Seo, Dongryeoul Bae, Ji-Hee Park, Saeed Khan, Kidon Sung
Clostridium perfringens causes diarrhea and other diseases in animals and humans. We investigated the prevalence, toxin gene profiles, and antibiotic resistance of C. perfringens isolated from diarrheic and non-diarrheic dogs in two animal hospitals in Seoul, South Korea. Fecal samples were collected from clinically diarrheic (DD; n= 49) and non-diarrheic dogs (ND; n= 34). C. perfringens was isolated from 31 out of 49 DD (63%) and 21 out of 34 ND dogs (62%). All strains were positive for the α toxin gene, but not for the β , ε , and ι toxin genes; therefore, all the strains were identified as type A C...
February 27, 2018: Journal of Veterinary Science
Pedro Melendez, Veronica Gomez, Hans Bothe, Francisco Rodriguez, Juan Velez, Hernando Lopez, Julian Bartolome, Louis Archbald
The objective of this study was to compare ovulation rate, number of large ovarian follicles, plasma progesterone (P4) and non-esterified fatty acids (NEFA) concentrations between lame (n=10) and non-lame (n=10) lactating Holstein cows. The study was conducted in an organic dairy farm and cows were evaluated by ultrasonography and blood sampling every 3 days from 30 days postpartum during a period of 34 days. Cows which became lame during the first 30 days postpartum that experienced a lower ovulation rate were determined by the presence of a corpus luteum (50% for lame cows and 100% for non-lame cows, P ≤ 0...
February 27, 2018: Journal of Veterinary Science
Anukul Taweechaipaisankul, Geon A Kim, Jun-Xue Jin, Su Cheong Yeom, Byeong Chun Lee
Due to their close similarities with humans in anatomy, physiology and genetics and handling advantages, miniature pigs are becoming a very attractive model for biomedical research. The purpose of this study was to establish and evaluate blood type O cells derived from Korean native pig (KNP), a typical miniature pig breed in Korea. Total 10 cell lines derived from 8 KNP piglets, adult KNP female pig (kidney and ear tissues) were established. To confirm the blood type O, genomic DNA, fucosyltransferase (FUT) expression and immunofluorescence staining were examined...
February 27, 2018: Journal of Veterinary Science
Katharine Roque, KyungMin Shin, JiHoon Jo, GyeongDong Lim, EunSeob Song, SoJung Shin, Ravi Gautam, JaeHee Lee, YeonGyeong Kim, AhRang Cho, ChangYul Kim, HyunJi Kim, MyungSook Lee, Hyeong-Geu Oh, Byung-Chul Lee, JungHee Kim, Kwang-Ho Kim, HyunKyu Jeong, HyoungAh Kim, Yong Heo
The indoor environment in animal husbandry is inevitably contaminated with endotoxin. Endotoxin exposure associates with various inflammatory illnesses in animals. The present cross-sectional study evaluated the relationship between the degree of endotoxin exposure and the cellular and humoral immune profiles of fattening pigs. Blood samples were taken from the jugular vein of 47 pigs from ten different pig farms in Korea. Whole blood cell counts and plasma immunoglobulin classes were measured. The peripheral blood mononuclear cells were stimulated in vitro with Concanavalin A for 48 hours and the cytokines released into the culture supernatants were measured...
January 25, 2018: Journal of Veterinary Science
Jonathan Lalsiamthara, Gayeon Won, John Hwa Lee
An anti-Brucella vaccine candidate which comprises of purified Brucella lipopolysaccharide and cocktail of four Salmonella Typhimurium (ST)-Brucella vectors was reported earlier. Each vector constitutively express highly conserved Brucella antigens (rB), viz. lumazine synthase (BLS), proline racemase subunit A (PrpA), outer membrane protein-19 (Omp19), and Cu-Zn superoxide dismutase (SOD). The present study determined relative level of protection conferred by each single strain. Upon virulent challenge, the challenge strain was recovered most abundantly in non-immunized control mice and the ST-Omp19, ST-BLS, LPS, and ST-SOD immunized mice groups showed much less burden...
January 25, 2018: Journal of Veterinary Science
Dan Wang, Shuhong Sun, Mohammad Heidari
To provide insights into the role of innate immune responses in vaccine-mediated protection, we investigated the effect of Marek's disease (MD) vaccine, Rispens/CVI988, on the expression pattern of selected genes associated with activation of macrophages in MD-resistant and susceptible chicken lines. Upregulation of IFN-γ, IL-1β, IL-8, and IL-12 at different time points post inoculation (dpi) revealed activation of macrophages in both chicken lines. Strong immune response was induced in cecal tonsils of the susceptible line at 5 dpi...
January 25, 2018: Journal of Veterinary Science
Daria Matczyńska, Daniel Sypniewski, Sabina Gałka, Dagna Sołtysik, Tomasz Loch, Ewa Nowak, Zdzisław Smorąg, Ilona Bednarek
Molecular characterization of the SLA (Swine Leukocyte Antigen) genes is important for understanding the immune responses between swine-donor and human-recipient in course of xenotransplantation. Explanation of association between alleles of SLA class I genes, type of pigs genetic modification, the PERV viral titer, and its subtypes may shed light on the nature of xenograft acceptance or rejection and the safety of xenotransplantation. No significant differences in PERV gag RNA level between transgenic and non-transgenic pigs were noted, likewise the type of applied transgene had no impact on PERV viremia...
January 25, 2018: Journal of Veterinary Science
Rui Zhu, Xueqin Yang, Jianjun Zhang, Danwen Xu, Jiawen Fan, Huoying Shi, Shifeng Wang, Xiufan Liu
H9N2 subtype avian influenza virus is a great threat to the Chinese poultry industry, even with annual vaccination. Waterfowl can be asymptomatically infected with the H9N2 virus. In this study, three strains of the H9N2 avian influenza virus, designated A/Goose/Jiangsu/YZ527/2011 (H9N2, Gs/JS/YZ527/11), A/Goose/Jiangsu/SQ119/2012 (H9N2, Gs/JS/SQ119/12), and A/Goose/Jiangsu/JD564/2012 (H9N2, Gs/JS/JD564/12), were isolated from domestic geese. Molecular characterization of the three isolates showed that the Gs/JS/YZ527/11 virus is a double-reassortant virus, combining the genes of A/Quail/Hong Kong/G1/97 (H9N2, G1/97) - like and A/Chicken/Shanghai/F/98 (H9N2, F/98) - like; the Gs/JS/SQ119/12 virus is a triple-reassortant virus derived from G1/97 - like, F/98 - like, and A/Duck/Shantou/163/2004 (H9N2, ST/163/04) - like...
January 25, 2018: Journal of Veterinary Science
Sayed Samim Rahpaya, Shinobu Tsuchiaka, Mai Kishimoto, Mami Oba, Yukie Katayama, Yuki Nunomura, Saki Kokawa, Takashi Kimura, Atsushi Kobayashi, Yumi Kirino, Tamaki Okabayashi, Nariaki Nonaka, Hirohisa Mekata, Hiroshi Aoki, Mai Shiokawa, Moeko Umetsu, Tatsushi Morita, Ayako Hasebe, Keiko Otsu, Tetsuo Asai, Tomohiro Yamaguchi, Shinji Makino, Yoshiteru Murata, Ahmad Jan Abi, Tsutomu Omatsu, Tetsuya Mizutani
The bovine abortion, diarrhea, and respiratory disease complexes, caused by infectious agents, result in high and significant economic losses for the cattle industry. These pathogens are likely transmitted by various vectors and reservoirs including insects, birds and rodents. However, experimental data that supports this possibility are scarce. We collected total 117 samples and screened for 44 bovine abortive, diarrheal and respiratory disease complex pathogens by using Dembo-PCR, based on TaqMan real-time PCR...
December 28, 2017: Journal of Veterinary Science
Seok Hee Lee, Erif Maha Nugraha Setyawan, Yoo Bin Choi, Jeong Chan Ra, Sung Keun Kang, Geon A Kim, Byeong Chun Lee
Adipose stem cells (ASCs) are an attractive source of stem cells with therapeutic applicability in various fields for regenerating damaged tissues because they show stemness characteristics. However, little investigation has been done to evaluate adverse responses caused by human ASCs therapy. Therefore, in the present study, a clinical assessment after human ASCs transplantation in dogs was investigated. A total of 12 healthy male dogs were selected and divided into 4 groups: 1) saline infusion and bolus groups, and 2) ASCs infusion and bolus groups...
December 28, 2017: Journal of Veterinary Science
Yong-Hyun Kim, Byung-Joo Park, Hee-Seop Ahn, Sang-Hoon Han, Hyeon-Jeong Go, Dong-Hwi Kim, Joong-Bok Lee, Seung-Yong Park, Chang-Seon Song, Sang-Won Lee, In-Soo Choi
The zoonotic transmission of hepatitis E virus (HEV) is mostly mediated by HEV-3 and HEV-4, and domestic pigs are an important reservoir for these genotypes. A survey of 14 pig farms in Korea revealed HEV RNA in 30 of 148 (20.3%) fecal samples. HEV-3a and HEV-4c subtypes were identified from five (36%) and two (14%) pig farms, respectively. Phylogenetic analysis indicated that the isolated HEV strains were closely related with previously reported zoonotic strains in Korea. This genetic analysis can partially explain the possible source of the zoonotic transmission of HEV to humans in Korea...
December 28, 2017: Journal of Veterinary Science
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