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Journal of Veterinary Science

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https://www.readbyqxmd.com/read/29695146/isolation-and-characterization-of-a-korean-porcine-deltacoronavirus-strain-knu16-07
#1
Guehwan Jang, Seong-Hee Kim, Yoo Jin Lee, Seungjoon Kim, Du Sik Lee, Kyoung-Ki Lee, Changhee Lee
Porcine deltacoronavirus (PDCoV) has emerged in several pig-raising countries and has also been a causative pathogen associated with diarrheal diseases in South Korea since 2014. In the present study, we were able to isolate and cultivate one Korean PDCoV strain in cell culture and further investigated its pathogenicity. PDCoV-inoculated piglets showed watery diarrhea accompanied by acute enteritis in the natural host. Sequencing analysis demonstrated genetic stability of KNU16-07 for at least thirty serial passages...
April 26, 2018: Journal of Veterinary Science
https://www.readbyqxmd.com/read/29695145/sciatic-nerve-leachate-of-cattle-causes-neuronal-differentiation-of-pc12-cells-via-erk1-2-signaling-pathway
#2
Ziqiang Zhang, Yumei Liu, Xuemin Zhu, Lan Wei, Jiamin Zhu, Ke Shi, Guotao Wang, Li Pan
Previous studies have shown that sciatic nerve has neurotrophic activity, and nerve regeneration, differentiation, axon outgrowth can be modulated by different sciatic nerve preparations. However, a number of animals have to be sacrificed to get sciatic nerves for making sciatic nerve preparations. Some studies have demonstrated that the role of sciatic nerve preparations in neural differentiation depends on the neurotrophins that schwann cells secrete, and these factors are highly conserved between different species...
April 26, 2018: Journal of Veterinary Science
https://www.readbyqxmd.com/read/29695144/speckle-tracking-analysis-of-myocardial-deformation-in-correlation-to-age-in-healthy-horses
#3
Heidrun Gehlen, Lisa-Marie Bildheim
An effect of aging on cardiac morphology and function has been shown in humans. In horses, cardiac wall-motion analysis has not been performed using two-dimensional speckle tracking (2D-ST) so far. Our study included 57 horses of different warmblood breeds between 3 and 30 years of age. Age had a significant influence on left ventricular (LVFW) systolic strain rate (P ≤ 0.05) and early diastolic relaxation (P ≤ 0.01). In the interventricular septum (IVS), systolic (P ≤ 0.01) and late diastolic (P ≤ 0...
April 26, 2018: Journal of Veterinary Science
https://www.readbyqxmd.com/read/29695143/transient-forebrain-ischemia-induces-impairment-in-cognitive-performance-prior-to-extensive-neuronal-cell-death-in-the-mongolian-gerbil-meriones-unguiculatus
#4
Tomohiro Kondo, Suguru Yoshida, Hiroaki Nagai, Ai Takeshita, Masaki Mino, Hiroshi Morioka, Takayuki Nakajima, Ken Takeshi Kusakabe, Toshiya Okada
In Mongolian gerbils, bilateral common carotid artery occlusion (BCCAO) for several minutes induces ischemia and delayed neuronal cell death in the Cornet d'Ammon 1 (CA1) region of the hippocampus by their incomplete circle of Willis. Neuronal cell death in the hippocapus and changes in behavior were examined after BCCAO was performed for 5 minutes in the gerbils. One day after BCCAO, the pyramidal neurons of the CA1 region of the hippocampus showed degenerative changes (clumped chromatin of the nuclei). Five days and 10 days after BCCAO, extensive neuronal cell death was observed in the hippocampal CA1 region...
April 26, 2018: Journal of Veterinary Science
https://www.readbyqxmd.com/read/29649860/identification-of-determinants-that-mediate-binding-between-tembusu-virus-and-the-cellular-receptor-heat-shock-protein-a9
#5
Dongmin Zhao, Qingtao Liu, Xinmei Huang, Huili Wang, Kaikai Han, Jing Yang, Keran Bi, Yuzhuo Liu, Lijiao Zhang, Yin Li
Heat shock protein A9 (HSPA9), a member of the heat shock protein family, is a putative receptor for tembusu virus (TMUV). Using Western blot and co-immunoprecipitation assay, E protein domains I and II were identified as the functional domains that are to facilitate HSPA9 binding. Twenty five over-lapping peptides covering the domains I and II sequence were synthesized and analyzed by HSPA9 binding assay. Two peptides showed the capability of binding to HSPA9. Dot blot assay of truncated peptides indicated that amino acid residues 19 to 22 and 245 to 252 of E protein constitute the minimal motifs required for TMUV binding to HSPA9...
April 12, 2018: Journal of Veterinary Science
https://www.readbyqxmd.com/read/29649859/comparison-of-electrophysiological-properties-of-two-types-of-pre-sympathetic-neurons-present-intermingled-in-hypothalamic-paraventricular-nucleus
#6
Yiming Shen, Seong Kyu Han, Pan Dong Ryu
The hypothalamic paraventricular nucleus (PVN) contains two types of neurons projecting to either the rostral ventrolateral medulla (PVNRVLM ) or the intermediolateral horn (IML) of the spinal cord (PVNIML ). These two neuron groups are present intermingled in the same subdivisions of the PVN, and differentially regulate sympathetic outflow. However, the electrophysiological evidence supporting such functional differences is largely lacking. Here, we compared the electrophysiological properties of these neurons by using patch clamp and retrograde tracing techniques...
April 12, 2018: Journal of Veterinary Science
https://www.readbyqxmd.com/read/29649858/pharmacokinetics-of-enrofloxacin-hcl-2h-2-o-enro-c-in-dogs-and-pk-pd-monte-carlo-simulations-against-leptospira-sp
#7
Hector Sumano, Luis Ocampo, Graciela Tapia, C de Jesus Mendoza, Lilia Gutierrez
Pharmacokinetics/pharmacodynamics (PK/PD) ratios of reference enrofloxacin (Enro-R) and enrofloxacin as HCl-2H2 O (Enro-C), as well as Monte Carlo simulations based on composite MIC50 and MIC90 vs. Leptospira sp., were carried out in dogs after their IM and oral administration (10 mg/kg). Plasma determination of enrofloxacin was achieved by means of high performance liquid chromatography (HPLC). Maximum plasma concentration values after oral administration were 1.47 ± 0.19 µg/mL and 5.3 ± 0.84 µg/mL for Enro-R and Enro-C, respectively, and 1...
April 12, 2018: Journal of Veterinary Science
https://www.readbyqxmd.com/read/29649857/oxidative-stress-and-hepatic-injury-induced-in-mice-fed-with-sarcocystis-hirsuta-cyst-extract
#8
Maryam Sasani, Reza Nabavi, Mohammad Reza Hajinezhad, Parisa Hasanein
We studies toxic effects of Sarcocystis hirsuta cyst extract in mice. Degenerative changes were found in the mice received fresh, frozen and heated S. hirsuta cyst extract. There were increases in the levels of serum aspartate aminotransferase and alanine aminotransferase (AST and ALT) as well as hepatic and brain malondialdehyde (MDA) levels with a concomitant decrease in catalase (CAT) and superoxide dismutase (SOD) activities of mice received fresh and frozen S. hirsuta extracts. Gavage feeding of heated S...
April 12, 2018: Journal of Veterinary Science
https://www.readbyqxmd.com/read/29649856/kilovoltage-radiotherapy-for-companion-animals-dosimetric-comparison-of-300-kv-450-kv-and-6-mv-x-ray-beams
#9
Jaehyeon Seo, Jaeman Son, Yeona Cho, Nohwon Park, Dong Wook Kim, Jinsung Kim, Myonggeun Yoon
Radiotherapy for the treatment of cancer in companion animals is currently administered using megavoltage X-ray machines. Because these machines are expensive, most animal hospitals do not perform radiotherapy. This study evaluated the ability of relatively inexpensive kilovoltage X-ray machines to treat companion animals. A simulation study based on a treatment planning system was performed for tumors of the brain (non-infectious meningoencephalitis), nasal cavity (malignant nasal tumors), forefoot (malignant muscular tumors), and abdomen (malignant intestinal tumors)...
April 12, 2018: Journal of Veterinary Science
https://www.readbyqxmd.com/read/29649855/a-salmonella-typhi-ghost-induced-by-the-e-gene-of-phage-%C3%AF-x174-stimulates-dendritic-cells-and-efficiently-activates-the-adaptive-immune-response
#10
Gayeon Won, Seong Kug Eo, Sang-Youel Park, Jin Hur, John Hwa Lee
We previously genetically engineered a Salmonella Typhi bacterial ghost (STG) as a novel inactivated vaccine candidate against typhoid fever. The underlying mechanism employed by the ghost to stimulate the adaptive immune response remains to be investigated. In this study, we aimed to evaluate the immunostimulatory effect of STG on mouse bone marrow-derived dendritic cells (BMDCs) and its activation of the adaptive immune response in vitro . Immature BMDCs were stimulated with STG, which efficiently stimulated maturation events in BMDCs, as indicated by up-regulated expression of CD40, CD80, and MHC-II molecules on CD11+ BMDCs...
April 12, 2018: Journal of Veterinary Science
https://www.readbyqxmd.com/read/29649854/effect-of-superoxide-dismutase-catalase-and-glutathione-peroxidase-supplementation-in-the-extender-on-chilled-semen-of-fertile-and-hypofertile-dogs
#11
Chiara Del Prete, Francesca Ciani, Simona Tafuri, Maria Pia Pasolini, Giovanni Della Valle, Veronica Palumbo, Lucia Abbondante, Antonio Calamo, Vincenza Barbato, Roberto Gualtieri, Riccardo Talevi, Natascia Cocchia
This study investigated the correlation between oxidative stress status and main canine sperm parameters and the effect of addition of Superoxide Dismutase (SOD), Catalase (CAT) and Glutathione Peroxidase (GPx) combination in egg yolk tris-citrate glucose (EYT-G) extender on semen during 10 days of storage at 4° C. Ten Boxer dogs were included in this study and divided in two groups, fertile (F) and hypofertile (H), depending on pregnancy and live birth rates in the previous year. The semen evaluation was performed at the day of collection (D0) and after 5 (D5) and 10 (D10) days of cooled storage at 5° C...
April 12, 2018: Journal of Veterinary Science
https://www.readbyqxmd.com/read/29510474/towards-the-development-of-a-one-dose-classical-swine-fever-subunit-vaccine-antigen-titration-onset-and-duration-of-immunity
#12
Rachel Flores Madera, Lihua Wang, Wenjie Gong, Yulia Burakova, Sterling Buist, Jerome Nietfeld, Jamie Henningson, Ada G Cino Ozuna, Changchun Tu, Jishu Shi
The highly contagious classical swine fever (CSF) remains a major trade and health problem in the pig industry, causing large economic losses worldwide. Modified live vaccines (MLV), commonly derived from the attenuated CSF virus (CSFV) C-strain, have been routinely used to control the disease in CSF-endemic countries. However, to completely eradicate the disease, a potent, safe and non-infectious CSF vaccine should be easily accessible and available. This study aims to develop a cost-effective, non infectious CSF subunit vaccine that can elicit rapid and long lasting immunity...
March 6, 2018: Journal of Veterinary Science
https://www.readbyqxmd.com/read/29510473/unilateral-rostral-mandibulectomy-for-gingival-vascular-hamartoma-in-two-calves
#13
Takeshi Tsuka, Yoshiharu Okamoto, Naoki Yamamoto, Keiji Hayashi, Takehito Morita, Yuji Sunden, Yusuke Murahata, Kazuo Azuma, Tomohiro Osaki, Norihiko Ito, Tomohiro Imagawa
A 2-month-old female Holstein calf and a 5-month-old female Japanese black calf presented with a gingival vascular hamartoma located in the interdental space between the second and third mandibular incisors in the right and left mandibles, respectively. Osteolytic changes appeared within the mandibular bones adjacent to the masses on radiographic or computed tomographic images. The masses were removed along with affected mandibular bone by unilateral rostral mandibulectomy. Two cases exhibited a normal appetite and grew normally, with no cosmetic changes and recurrences...
March 6, 2018: Journal of Veterinary Science
https://www.readbyqxmd.com/read/29510472/an-enhanced-immunochromatographic-strip-test-using-colloidal-gold-nanoparticles-labled-dual-type-n-proteins-for-detection-of-antibodies-to-porcine-reproductive-and-respiratory-syndrome-virus
#14
Ji Eun Yu, In-Ohk Ouh, Hyeonjeong Kang, Hye-Young Lee, Kwang-Myun Cheong, In-Soo Cho, Sang-Ho Cha
Porcine reproductive and respiratory syndrome (PRRS) has been proposed to be one of the most important infectious diseases causing serious economic loss in swine industry worldwide. Due to its increasing genetic diversity, a rapid and accurate diagnosis is critical in PRRS control. The immunochromatographic strip test (ICST) has been recognized as a type of immunoassay with speed and convenience. In this study, the on-site diagnostic method with immunochromatographic assay was developed for detection of PRRSV-specific antibodies based on colloidal gold nanoparticles-labeled dual-type nucleocapsid (N) proteins encoded by open reading frame (ORF) 7, and evaluated using 991 field samples from pig farms and 66 serum samples from experimentally PRRSV-inoculated pigs...
March 6, 2018: Journal of Veterinary Science
https://www.readbyqxmd.com/read/29486539/changes-in-hoof-kinetics-and-kinematics-at-walk-in-response-to-hoof-trimming-using-a-pressure-plate
#15
Babak Faramarzi, An Nguyen, Fanglong Dong
Appropriate hoof preparation and symmetry are linked to the wellbeing of the horse. Previous studies have shown the efficacy of the pressure plates (PP) in delivering objective biomechanical analysis. We aimed to assess the effect of hoof trimming on hoof biomechanics using a PP. Nine clinically sound Arabian horses were walked across the PP while foot strike was recorded by a digital camera. Kinetic and kinematic parameters were recorded before and after trimming. P values ≤ 0.05 were considered significant...
February 27, 2018: Journal of Veterinary Science
https://www.readbyqxmd.com/read/29486538/hepatobiliary-diseases-in-buffaloes-bubalus-bubalis-clinical-laboratory-and-ultrasonographic-findings
#16
Arafat Khalphallah, Nasr-Eldin M Aref, Ashraf M Abu-Seida, Enas Elmeligy, Sara A Bayoumi, Al-Lethie A Al-Lethie, Doaa Salman
This study describes the ultrasonographic findings of five hepatobiliary diseases in buffaloes. Fifty buffaloes including; 20 clinically normal and 30 hepatobiliary diseased buffaloes were enrolled in this study. Complete clinical, radiographic and ultrasonographic examinations and laboratory analyses were conducted. RESULTS: Focal parenchymal lesions including liver abscess (N=12) and hepatic cyst (N=6), diffuse parenchymal lesion (hepatobiliary cirrhosis, N=5) and obstruction of hepatobiliary passages including cholestasis (N=4) and hepatocholelithiasis (N=3) were successfully imaged by ultrasound...
February 27, 2018: Journal of Veterinary Science
https://www.readbyqxmd.com/read/29486537/porcine-intestinal-lymphoid-tissues-synthesize-estradiol
#17
Chan Jin Park, Heehyen Kim, Jooyoung Jin, Radwa Barakat, Po-Ching Lin, Jeong Moon Choi, CheMyong Jay Ko
17β-estradiol is synthesized primarily in the gonads of both sexes and regulates the development and function of reproductive organs. Lately, we discovered that intestinal lymphocyte homeostasis is regulated by estradiol synthesized de novo in the endothelial cells of high endothelial venule of mesenteric lymph node and Peyer's patches in mice. This finding prompted us to hypothesize that the high endothelial venule of intestinal lymphoid tissues is the site of estradiol synthesis across the species. In this study, we determined if estradiol is synthesized in the porcine intestinal lymphoid tissues using adolescent piglets...
February 27, 2018: Journal of Veterinary Science
https://www.readbyqxmd.com/read/29486536/non-invasive-quantification-of-hepatic-fat-content-in-healthy-dogs-using-proton-magnetic-resonance-spectroscopy-and-dual-gradient-echo-magnetic-resonance-imaging
#18
Francesca Del Chicca, Andrea Schwarz, Dieter Meier, Paula Grest, Annette Liesegang, Patrick Robert Kircher
Objective of the present study is to describe two non-invasive methods of fat quantification in the normal canine liver using magnetic resonance imaging and spectroscopy. Eleven adult beagle dogs were anesthetized and underwent magnetic resonance examination of the cranial abdomen including morphologic imaging, a modified Dixon (mDixon) dual gradient echo sequence and proton magnetic resonance spectroscopy (1 H MRS). In addition, ultrasonographic examination of the liver, fine needle aspirates and liver biopsies were performed, and hepatic triglyceride content was assayed...
February 27, 2018: Journal of Veterinary Science
https://www.readbyqxmd.com/read/29486535/phenotypic-and-genotypic-analyses-of-an-attenuated-porcine-reproductive-and-respiratory-syndrome-virus-strain-after-serial-passages-in-cultured-porcine-alveolar-macrophages
#19
Seung-Chul Lee, Sunhee Lee, Gun-Woo Yoo, Hwan-Won Choi, Yun-Hee Noh, Chang Eon Park, Jae-Ho Shin, In-Joong Yoon, Shien-Young Kang, Changhee Lee
Porcine reproductive and respiratory syndrome virus (PRRSV) is a globally ubiquitous swine viral pathogen that causes major economic losses worldwide. We previously reported an over-attenuated phenotype of a cell-adapted PRRSV strain CA-2-P100 in vivo . In the present study, CA-2-P100 was serially propagated in cultured porcine alveolar macrophage (PAM) cells for up to 20 passages to obtain the derivative strain CA-2-MP120. Animal inoculation studies revealed that both CA-2-P100 and CA-2-MP120 had decreased virulence, eliciting similar weight gain, body temperatures, and histopathologic lesions as in the negative control group...
February 27, 2018: Journal of Veterinary Science
https://www.readbyqxmd.com/read/29486534/the-influence-of-bronchoalveolar-lavage-on-thoracic-radiography-in-the-horse
#20
Ann Kristin Barton, Thorben Schulze, Marcus G Doherr, Heidrun Gehlen
Bronchoalveolar lavage (BAL) and thoracic radiography are routinely performed diagnostic procedures. We hypothesized that the BAL increases the interstitial opacity of caudoventral and caudodorsal thoracic radiographs. 53 horses, including 12 clinic owned and 41 horses of a referral-hospital population, were classified as healthy controls (n=12), severe (RAO, n=12) or mild-to-moderate equine asthma (IAD, n=21) according to the results of a clinical scoring system. 4 blinded and randomized thoracic radiographs of each horse were scored by two observers, who were also asked to identify the image as obtained before or after the BAL procedure...
February 27, 2018: Journal of Veterinary Science
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