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Journal of Veterinary Science

Sayed Samim Rahpaya, Shinobu Tsuchiaka, Mai Kishimoto, Mami Oba, Yukie Katayama, Yuki Nunomura, Saki Kokawa, Takashi Kimura, Atsushi Kobayashi, Yumi Kirino, Tamaki Okabayashi, Nariaki Nonaka, Hirohisa Mekata, Hiroshi Aoki, Mai Shiokawa, Moeko Umetsu, Tatsushi Morita, Ayako Hasebe, Keiko Otsu, Tetsuo Asai, Tomohiro Yamaguchi, Shinji Makino, Yoshiteru Murata, Ahmad Jan Abi, Tsutomu Omatsu, Tetsuya Mizutani
The bovine abortion, diarrhea, and respiratory disease complexes, caused by infectious agents, result in high and significant economic losses for the cattle industry. These pathogens are likely transmitted by various vectors and reservoirs including insects, birds and rodents. However, experimental data that supports this possibility are scarce. We collected total 117 samples and screened for 44 bovine abortive, diarrheal and respiratory disease complex pathogens by using Dembo-PCR, based on TaqMan real-time PCR...
December 28, 2017: Journal of Veterinary Science
Seok Hee Lee, Erif Maha Nugraha Setyawan, Yoo Bin Choi, Jeong Chan Ra, Sung Keun Kang, Geon A Kim, Byeong Chun Lee
Adipose stem cells (ASCs) are an attractive source of stem cells with therapeutic applicability in various fields for regenerating damaged tissues because they show stemness characteristics. However, little investigation has been done to evaluate adverse responses caused by human ASCs therapy. Therefore, in the present study, a clinical assessment after human ASCs transplantation in dogs was investigated. A total of 12 healthy male dogs were selected and divided into 4 groups: 1) saline infusion and bolus groups, and 2) ASCs infusion and bolus groups...
December 28, 2017: Journal of Veterinary Science
Yong-Hyun Kim, Byung-Joo Park, Hee-Seop Ahn, Sang-Hoon Han, Hyeon-Jeong Go, Dong-Hwi Kim, Joong-Bok Lee, Seung-Yong Park, Chang-Seon Song, Sang-Won Lee, In-Soo Choi
The zoonotic transmission of hepatitis E virus (HEV) is mostly mediated by HEV-3 and HEV-4, and domestic pigs are an important reservoir for these genotypes. A survey of 14 pig farms in Korea revealed HEV RNA in 30 of 148 (20.3%) fecal samples. HEV-3a and HEV-4c subtypes were identified from five (36%) and two (14%) pig farms, respectively. Phylogenetic analysis indicated that the isolated HEV strains were closely related with previously reported zoonotic strains in Korea. This genetic analysis can partially explain the possible source of the zoonotic transmission of HEV to humans in Korea...
December 28, 2017: Journal of Veterinary Science
Sang-Ik Oh, Jong Wan Kim, Ji-Youl Jung, Myeongju Chae, Yu-Ran Lee, Jong Ho Kim, ByungJae So, Ha-Young Kim
Streptococcus dysgalactiae subspecies equisimilis (SDSE) is an emerging pathogen in animals and humans. Here, we describe two clinical swine cases of SDSE infection presenting with lameness, neurological signs, or sudden death. Pathological examination indicated suppurative arthritis, encephalitis, and multifocal abscesses in the kidney and heart. β-hemolytic colonies from the joint samples of each case were identified as SDSE. The isolates showed low minimum inhibitory concentration values for β-lactams and presented the same virulence gene profile (slo-/sagA+/pSTKP8+)...
December 28, 2017: Journal of Veterinary Science
Hanqin Shen, Xiangcong Liu, Pengfei Zhang, Lei Wang, Yanling Liu, Leyi Zhang, Pengshuai Liang, Changxu Song
Recently, a novel atypical porcine pestivirus (APPV) was discovered in pig. In this study, two APPV strains, APPV-China/GZ01/2016 (GZ01) and APPV-China/GD-SD/2016 (GD-SD), were identified in two newborn piglet herds with congenital tremor (CT) from China. The open reading frame of these two strains shared 83.5% nucleotide identity. Phylogenetically, the APPV strains were divided into two groups and GZ01 belonged to Group I, while GD-SD belonged to Group II. High viral load was detected in the cerebellum (Cq〈26)...
December 28, 2017: Journal of Veterinary Science
Artur N Carreiro, João A R Alves Diniz, Joyce G de Souza, Débora V Fernandes de Araújo, Rômulo F Francelino Dias, Liliane M Dos Santos Azerêdo, Ediane F Rocha, Ana Yasha F de La Salles, Carlos E Peña Alfaro, Maria A Martins de Carvalho, Maria J Illera, Danilo J Ayres de Menezes
The objective of the present study was to follow and characterize the morphological alterations in ovaries of agouti (Dasyprocta prymnolopha), reared in captivity, by abdominal ultrasonography. All the animals were submitted daily to vaginal cytology to identify the current cycle phases. For each phase, ultrasound examinations were carried out to identify and describe the morphology of both the ovaries regarding each phase of the estrus cycle, establishing topographic parameters in an ultrasound window to locate them...
December 28, 2017: Journal of Veterinary Science
Xiukun Sui, Ting Xin, Xiaoyu Guo, Hong Jia, Ming Li, Xintao Gao, Jing Wu, Yitong Jiang, Willems L, Hongfei Zhu, Shaohua Hou
Two strains of PRRSVs were isolated in 2006 and 2016, respectively, and designated as FZ06A and FZ16A. Through the inoculation experiments, it was shown that FZ06A caused 100% morbidity and 60% mortality, while FZ16A caused 100% morbidity without death. By using genomic sequence and phylogenetic analyses, close relation of Chinese highly pathogenic PRRSV strain with FZ06A and FZ16A strains was achieved. Based on the achieved results, multiple genomic variations in Nsp2, a unique N-glycosylation site (N³³→K³³), and a K151 AA substitution for virulence in the GP5 of FZ16A were detected except of 30 amino acids (AA) deletion in the NSP2-coding region...
December 28, 2017: Journal of Veterinary Science
Xiuying Zhao, Yihan Xian, Chao Wang, Liren Ding, Xianglong Meng, Weiyun Zhu, Suqin Hang
This study aimed to understand the effect of Tryptophan (Trp) on gut hormones secretion as well as the roles of the CaSR and its downstream signaling pathway in gut hormones secretion using swine duodenal perfusion in vitro. Swine duodenum was perfused with Krebs-Henseleit buffers (KHB) as a basal solution. Various concentrations (0, 10 mM and 20 mM) of Trp were applied to investigate the effect of Trp on gut hormones secretion. A CaSR antagonist was used to detect the involvement of CaSR and its signal molecules...
December 28, 2017: Journal of Veterinary Science
Francesca Perondi, Claudio Brovida, Gianila Ceccherini, Grazia Guidi, Ilaria Lippi
Three dogs were evaluated for severe hyperproteinemia and hyperglobulinemia secondary to Leishmania infantum. Double filtration plasmapheresis (DFPP) was performed in two dogs at day 1, 2 and 6 after presentation (T0). The third dog received two treatments of DFPP at day 1 and 3, and eleven hemodialysis treatments to support renal function. A significant reduction in serum total protein (p〈0.0001), alpha-1 (p〈0.023), alpha-2 (p〈0.018), gamma globulins (p=0.0105), and a significant increase in albumin/globulin ratio (p=0...
December 28, 2017: Journal of Veterinary Science
Seon-Ung Hwang, Kiyoung Eun, Junchul David Yoon, Hyunggee Kim, Sang-Hwan Hyun
Transgenic (TG) pigs are an important model for biomedical research, including disease modeling, pharmaceutical toxicity testing, and regenerative medicine. Here, we constructed 2 vector systems using the promoter of the pig glial fibrillary acidic protein (pGFAP) gene, which is an astrocyte cell marker. We established donor TG fibroblasts with pGFAP-CreERT2; LCMV-EGFPLoxP. We evaluated the effect of the transgenes on TG-SCNT embryo development. The cleavage rates were not significantly different among control and transgene-donor group...
December 28, 2017: Journal of Veterinary Science
Qing Wang, Mianmian Chen, Wei Zhang
Similar to other bacterial pathogens, current studies of the pathogenesis of Riemerella anatipestifer (RA) are focused mainly on in vitro culture conditions. To provide a further understanding of the pathogenesis of RA in vivo, bacterial RNA was extracted from overnight tryptic soy broth cultures (in vitro) and from the blood of infected ducks (in vivo) for comparative RNA-seq analysis. In total, 682 upregulated genes were identified in vivo. Among these upregulated genes, a signal transduction response regulator (ArsR) and a signal transduction histidine kinase (SthK) were predicted located on the same operon...
December 28, 2017: Journal of Veterinary Science
June Bong Lee, Dalmuri Han, Hyung Tae Lee, Seon Mi Wi, Jeong Hoon Park, Jeong Woo Jo, Young-Jae Cho, Tae-Wook Hahn, Sunjin Lee, Byunghak Kang, Hyo Sun Kwak, Jonghyun Kim, Jang Won Yoon
Herein, we report the pathogenic and phylogenetic characteristics of seven Shiga toxin (Stx)-producing Escherichia coli (STEC) isolates from 434 retail meats in Korea during 2006-2012. The experimental analyses revealed that all the isolates (i) were identified as non-O157 STEC, including O91:H14 (3 isolates), O121:H10 (2), O91:H21 (1), and O18:H20 (1), (ii) carried diverse Stx subtype genes (stx1, stx2c, stx2e, or stx1+stx2b) whose expression levels were varied strain by strain, and (iii) lacked the locus of enterocyte effacement (LEE) pathogenicity island, a major virulence factor of STEC, but possessed one or more alternative virulence genes encoding cytotoxins (Cdt and SubAB) and/or adhesins (Saa, Iha, and EcpA)...
December 28, 2017: Journal of Veterinary Science
Zhao Guimin, Wang Hongmei, Hou Peili, He Chengqiang, He Hongbin
Paratuberculosis (Johne's disease) is a chronic debilitating disease of domestic and wild ruminants. Quick diagnosis could facilitate control; however widespread point-of-care testing is infrequently done due to the lack of robust method. Isothermal recombinase polymerase amplification (RPA) technique has emerged as a novel DNA amplify assay for use in rapid diagnosis. Here, an RPA combined with lateral flow dipstick (LFD) assay was developed to estimate DNA from M.paratuberculosis. First, the specificity and sensitivity of RPA-nfo primer and probe sets were assessed...
December 28, 2017: Journal of Veterinary Science
Jongsu Lim, Youngmin Yoon, Taesung Hwang, Hee Chun Lee
This study was carried out to derive reference value of normal spine indices, and to evaluate the feasibility of the indices for computed tomography (CT). CT images and magnetic resonance imaging were acquired from clinically normal 12 beagle dogs for normal group and 50 dogs with 56 spinal disorders for patient group. Image acquisition regions were cervical spine (C2 to T1), thoracic spine (T1-T13) and lumbar spine (L1-L7). The measured indices are as follows; the ratio of width to height of spinal cord include dura matter (CR) and vertebral foramen (FR), and the ratio of cross sectional area of spinal cord to vertebral foramen (CFAR)...
November 23, 2017: Journal of Veterinary Science
Jong-Hyeon Park, Dongseob Tark, Kwang-Nyeong Lee, Ji-Eun Chun, Hyang-Sim Lee, Young-Joon Ko, Soo-Jeong Kye, Yong-Joo Kim, Jae-Ku Oem, Soyoon Ryoo, Sung-Bin Lim, Seo-Yong Lee, Joo-Hyung Choi, Mi-Kyeong Ko, Su-Hwa You, Myoung-Heon Lee, Byounghan Kim
On December 3, 2014, a type O foot-and-mouth disease (FMD) outbreak occurred in South Korea. Although vaccinations were administered, cases of FMD increased steadily for five months and reached 185 cases by April 2015. Most of the affected animals were pigs, which are vulnerable to vaccinations. The FMD virus belonged to the South-East Asia (SEA) topotype that had been observed three times in Korea from April 2010 to July 2014. However, the virus in December 2014 had a unique difference in a partial deletion of the 5' noncoding region, which had not been seen in the previous SEA topotype isolates that were identified in Korea...
November 23, 2017: Journal of Veterinary Science
Yiming Shen, Janardhan P Bhattarai, Soo Joung Park, Gyu Seung Lee, Pan Dong Ryu, Seong Kyu Han
It has been reported that Korean red ginseng (KRG) as a valuable and important traditional medicine has various effects on central nervous system suggesting its complicated actions. Paraventricular nucleus (PVN) neurons of hypothalamus play a critical role for stress responses and hormone secretions. Although many studies have reported the action mechanisms of KRG on various cell and systems, the direct membrane effects of KRG on the PVN neurons which are involved in stress response are still poorly understood...
November 23, 2017: Journal of Veterinary Science
Soo Chan Lee, Jae Kwan Jeong, In Soo Choi, Hyun Gu Kang, Young Hun Jung, Soo Bong Park, Ill Hwa Kim
We determined the threshold proportion of polymorphonuclear leukocytes (PMNs) for the diagnosis of cytological endometritis (CEM), the risk factors for this condition, and its impact on reproductive performance in dairy cows. Uterine cytology was performed on 407 Holstein cows 4 weeks postpartum to determine the proportions of endometrial cells and PMNs. A receiver operator characteristics curve was used to determine the threshold above which the PMN proportion affected the likelihood of cows conceiving by 200 days postpartum...
November 23, 2017: Journal of Veterinary Science
Jiyoung Park, Hae-Beom Lee, Seong Mok Jeong
This study compared effects of isoflurane inhalation (ISO) and propofol-remifentanil combined total intravenous anesthesia (TIVA) on oxygenation during thoracoscopic lung lobectomy with 30-minute one-lung ventilation (1LV). Thoracoscopic right middle lung lobectomy was performed in ten dogs divided into ISO and TIVA group and cardiopulmonary parameters were measured with blood gas analysis. Throughout the study, isoflurane was inhaled up to 1.5%, and the infusion rates of propofol and remifentanil were 0.2~0...
November 23, 2017: Journal of Veterinary Science
Ke Ding, Ke Shang, Zu-Hua Yu, Chuan Yu, Yan-Yan Jia, Lei He, Cheng-Shui Liao, Jing Li, Chun-Jie Zhang, Yin-Ju Li, Ting-Cai Wu, Xiang-Chao Cheng
Newcastle disease virus (NDV) and Salmonella Pullorum (S. Pullorum) have great damage to the poultry industry, but no perfect vaccine can protect them effectively until now. In this study, a recombinant-attenuated S. Pullorum strain secreting Newcastle disease virus hemagglutinin-neuraminidase (HN) protein, C79-13ΔcrpΔasd (pYA-HN), was constructed as a new bivalent vaccine candidate against Newcastle disease and S. Pullorum disease (PD) by the method of suicide plasmid pREasd-mediated bacteria homologous recombination...
October 13, 2017: Journal of Veterinary Science
Biruk Tesfaye Birhanu, Seung-Jin Lee, Na-Hye Park, Ju-Beom Song, Seung-Chun Park
Actinobacillus pleuropneumoniae is a gram-negative bacterium residing in the respiratory tract of pigs that causes porcine respiratory disease complex, which leads to significant losses in the pig industry worldwide. The incidence of drug resistance in this bacterium is increasing; thus, identifying new protein/gene targets for drug and vaccine development is critical. In this study, we used an in silico approach, utilizing several databases, including Kyoto Encyclopedia of Genes and Genomes (KEGG), the database of essential genes (DEG), DrugBank, and Swiss-Prot, to identify non-homologous essential genes and prioritize these proteins for their druggability...
October 13, 2017: Journal of Veterinary Science
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