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Journal of Veterinary Science

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https://www.readbyqxmd.com/read/30173499/pre-surgical-assessment-of-splenic-tumors-in-dogs-a-retrospective-study-of-57-cases-2012-2017
#1
Mokhyeon Lee, Jiyoung Park, Hojung Choi, Haebeom Lee, Seong Mok Jeong
The purpose of this study was to evaluate the clinical and imaging characteristics of splenic tumors and to establish guidelines for the pre-surgical assessment of splenic tumors. Fifty seven dogs that received total splenectomy for the treatment of splenic tumors were evaluated by medical records, hematologic results, diagnostic imaging results and histopathologic results. The maximum lesion size from ultrasonography was significantly different between malignant and benign tumors ( p = 0.002). There was a correlation between margination and type of splenic tumors ( p = 0...
August 30, 2018: Journal of Veterinary Science
https://www.readbyqxmd.com/read/30173498/novel-reassortants-of-clade-2-3-4-4-h5n6-highly-pathogenic-avian-influenza-viruses-possessing-genetic-heterogeneity-in-republic-of-korea-in-late-2017
#2
Yu-Na Lee, Sun-Ha Cheon, Soo-Jeong Kye, Eun-Kyoung Lee, Mingeun Sagong, Gyeong-Beom Heo, Yong-Myung Kang, Hyun-Kyu Cho, Yong-Joo Kim, Hyun-Mi Kang, Myoung-Heon Lee, Youn-Jeong Lee
Novel H5N6 highly pathogenic avian influenza viruses (HPAIVs) were isolated from duck farms and migratory bird habitats in South Korea in November-December 2017. Genetic analysis demonstrated that at least two genotypes of H5N6 were generated through reassortment between clade 2.3.4.4 H5N8 HPAIVs and Eurasian low pathogenic avian influenza virus in migratory birds in late 2017, suggesting frequent reassortment of clade 2.3.4.4 H5 HPAIVs, and highlighting the need for systematic surveillance in Eurasian breeding grounds...
August 30, 2018: Journal of Veterinary Science
https://www.readbyqxmd.com/read/30173497/comparison-of-immunoadjuvant-activities-of-four-bursal-peptides-combined-with-h9n2-avian-influenza-virus-vaccine
#3
Cong Zhang, Jiangfei Zhou, Zhixin Liu, Yongqing Liu, Kairui Cai, Tengfei Shen, Chengshui Liao, Chen Wang
The bursa of Fabricius (BF) is the central humoral immune organ unique to birds. The bursal peptide BPs (BP-I, BP-II, BP-III, BP-IV) were isolated and identified from BF. In this study, the immunoadjuvant activities of BP-(I-IV) were examined in mice immunized with H9N2 avian influenza virus (AIV) vaccines. The results suggested that BP-I effectively enhanced cell-mediated immune responses, increased the secretion of Th1 (gamma interferon [IFN-γ]) and Th2 (IL-4)-type cytokines, and induced a better cytotoxic T-lymphocyte (CTL) response to the H9N2 virus...
August 30, 2018: Journal of Veterinary Science
https://www.readbyqxmd.com/read/30173496/comparison-of-two-techniques-for-transpharyngeal-endoscopic-auditory-tube-diverticulotomy-in-the-horse
#4
Drew W Koch, Jeremiah T Easley, Brad B Nelson, Jeremy J Delcambre, Erin G McCready, Eileen S Hackett
Auditory tube diverticula, also known as guttural pouches, are naturally occurring dilations of the auditory tube in horses that communicate with the nasopharynx through a small ostium. Infection and select other conditions can result in inflammation and narrowing of the nasopharyngeal ostium, which prevents drainage of fluid or egress of air and can lead to persistent infection or guttural pouch tympany. Auditory tube diverticulotomy allows continuous egress from the auditory tube diverticula and is a feature of disease treatment in horses, in which medical treatment alone is not successful...
August 30, 2018: Journal of Veterinary Science
https://www.readbyqxmd.com/read/30173495/neonatal-influenza-virus-infection-affects-myelination-in-the-recovered-mouse-brain
#5
Jin Hee Kim, Ji Eun Yu, Byung-Joon Chang, Sang-Soep Nahm
Influenza virus infection is a zoonosis that has great socioeconomic impact worldwide. Influenza infection induces respiratory symptoms, while influenza virus can infect brain and leave CNS sequelae. As children are more vulnerable to infection, they are at risk of long-term neurological effects once their brains are infected. We previously demonstrated that functional changes in hippocampal neurons were observed in mice recovered from neonatal influenza infection. In this study, we investigated the changes in myelination properties that could affect neural dysfunction...
August 30, 2018: Journal of Veterinary Science
https://www.readbyqxmd.com/read/30173494/molecular-prophage-typing-of-staphylococcus-aureus-isolates-from-bovine-mastitis
#6
Dae-Sung Ko, Won-Jin Seong, Danil Kim, Eun-Kyung Kim, Nam-Hyung Kim, Chung-Young Lee, Jae-Hong Kim, Hyuk-Joon Kwon
Staphylococcus aureus is one of the major pathogens causing bovine mastitis and foodborne disease associated with dairy products. To determine the genetic relationships between human and bovine or bovine isolates of S. aureus , various molecular methods have been used. Previously we developed an rpoB sequence typing (RSTing) method for molecular differentiation of S. aureus isolates and identification of RpoB -related antibiotic resistance. In this study, we performed spa typing and RSTing with 84 isolates from mastitic cows (22 farms, 72 cows, and 84 udders) and developed a molecular prophage typing (mPPTing) method for molecular epidemiological analysis of bovine mastitis...
August 30, 2018: Journal of Veterinary Science
https://www.readbyqxmd.com/read/30173493/molecular-phylogenetic-studies-on-clinical-bovine-piroplasmosis-caused-by-benign-theileria-in-shaanxi-province-china
#7
Jing Wang, Jiyu Zhang, Zhen Zhu, Xuzheng Zhou, Bing Li
A group of benign Theileria species, which is often referred to as Theileria orientalis/sergenti/buffeli , shows low pathogenicity in cattle. Here, we report the species of Theileria in cattle on a farm from China. We detected 6 genotypes that were categorized as types 1, 2, 3, 4, and 5 as well as an additional type 9 from the phylogenetic analysis of MPSP gene sequences. The new epidemiological features of the T . orientalis/sergenti/buffeli parasites in China reveal a greater diversity in the genetics of these species than had been previously thought...
August 30, 2018: Journal of Veterinary Science
https://www.readbyqxmd.com/read/30173492/correlation-of-spink1-gene-polymorphisms-in-canine-pancreatitis
#8
Ming-Huei Fan, Shih-Jen Chou, Zhi-Jia Zheng, Jui-Te Wu, Kun-Wei Chan
Pancreatitis is an exocrine pancreatic disease in dogs caused by inflammatory injury. The congenital factors and pathogenesis of pancreatitis is not clearly understood. We screened the canine PRSS1 gene in 34 dogs (27 dogs clinically diagnosed with pancreatitis and 7 dogs without pancreatitis as controls), and screened SPINK1 gene in 46 dogs (29 case dogs and 17 control dogs). Serum triglyceride (TG) concentrations were examined between two groups to study the correlation among gene polymorphisms, hypertriglyceridemia (HTG) and canine pancreatitis...
August 30, 2018: Journal of Veterinary Science
https://www.readbyqxmd.com/read/30173491/hexon-and-fiber-gene-changes-in-an-attenuated-fowl-adenovirus-isolate-of-malaysia-in-chickens-embryonated-eggs-and-its-infectivity-in-chickens
#9
M S Norfitriah, M Hair-Bejo, A R Omar, I Aini, M I Nurulfiza
Fowl adenovirus (FAdV) is distributed worldwide and caused economic losses in poultry industry. The objectives of this study were to determine the hexon and fiber gene changes in an attenuated FAdV isolate of Malaysia in specific pathogen free (SPF) chicken embryonated eggs (CEE) and its infectivity in commercial broiler chickens. SPF CEE was inoculated with 0.1mL FAdV inoculum via chorioallantoic membrane (CAM) for 20 consecutive passages. The isolate at passage 20 (E20) with virus titer of 108.7 TCID50 /mL was inoculated (0...
August 30, 2018: Journal of Veterinary Science
https://www.readbyqxmd.com/read/30041292/transforming-growth-factor-beta-1-upregulates-the-expression-of-hyaluronan-synthase-2-and-hyaluronan-synthesis-in-culture-models-of-equine-articular-chondrocytes
#10
Siriwan Ongchai, Oraphan Somnoo, Patiwat Kongdang, Siriporn Peansukmanee, Siriwan Tangyuenyong
We investigated the effect of transforming growth factor beta 1 (TGF-β1) on equine hyaluronan synthase 2 ( HAS2 ) gene expression and hyaluronan (HA) synthesis in culture models of articular chondrocytes. Equine chondrocytes were treated with TGF-β1 at different concentrations and time points in monolayer cultures. In three-dimensional cultures, chondrocyte-seeded gelatin scaffolds were cultured in chondrogenic media containing 10 ng/mL of TGF-β1. The amount of HA in conditioned media and in scaffolds was determined using ELISA...
July 20, 2018: Journal of Veterinary Science
https://www.readbyqxmd.com/read/30041291/evaluation-of-an-arthroscopic-stifle-lever-for-stifle-joint-distraction-in-toy-breed-dogs
#11
Ji Yong Park, Bum Soo Jeong, Yoon Seok Roh, Seong Mok Jeong, Hae Beom Lee
Arthroscopy has become an attractive modality in diagnoses and treatment of joint diseases in toy breed dogs. However, application of arthroscopy is limited by small joint space. Our objective was to evaluate the efficacy of a stifle lever for joint distraction during stifle arthroscopy in toy breed dogs. Paired stifles (n = 32 each) collected from 16 cadavers of toy breed dogs were randomly divided in two groups: the stifle lever group or the external manipulation group. All stifles underwent arthroscopic cranial cruciate ligament (CrCL) transection, and the visualization of the medial meniscus was evaluated...
July 20, 2018: Journal of Veterinary Science
https://www.readbyqxmd.com/read/30041290/evaluation-of-renal-injury-caused-by-acute-volume-replacement-with-hydroxyethyl-starch-130-0-4-or-ringer-s-lactate-solution-in-pigs
#12
David A Ferreira, Rita Cruz, Carlos Venâncio, Ana I Faustino-Rocha, Aura Silva, João R Mesquita, Ana Liza Ortiz, Helena Vala
This work aimed to evaluate the effects on renal tissue integrity after Hydroxyethyl starch (HES) 130/0.4 and Ringer's lactate (RL) administration in pigs under general anesthesia after acute bleeding. A total of 30 mL/kg of blood were passively removed from the femoral artery in two groups of Large White pigs, under total intravenous anesthesia with propofol and remifentanil. After bleeding, Group 1 ( n =11) received RL solution (25 mL/kg) and Group 2 ( n =11) received HES 130/0.4 solution (20 mL/kg). An additional Group 3 ( n =6) was not submitted to bleeding or volume replacement...
July 20, 2018: Journal of Veterinary Science
https://www.readbyqxmd.com/read/30041289/detection-and-genetic-characterization-of-porcine-circovirus-3-from-aborted-fetuses-and-pigs-with-respiratory-disease-in-korea
#13
Seong-Hee Kim, Ji-Young Park, Ji-Youl Jung, Ha-Young Kim, Yu-Ri Park, Kyoung-Ki Lee, Young-Soo Lyoo, Sang-Geon Yeo, Choi-Kyu Park
A novel porcine circovirus 3 (PCV3) was first detected in pigs showing porcine dermatitis and nephropathy-syndrome, reproductive failure, and multi-systemic inflammation in the US. This is a report of PCV3 as a potential etiological agent of clinical signs, reproductive failure and respiratory distress on Korean pig farms, based on in situ hybridization, pathological, and molecular findings. Confirmation of the presence of PCV3 may be increased coinfection patterns with other causative agents of disease in Korean pig herds, indicating the need for further systemic investigation of pathogenicity and of multiple infections with PCV2 genotypes and bacteria, and development of an effective PCV3 vaccine...
July 20, 2018: Journal of Veterinary Science
https://www.readbyqxmd.com/read/30041288/quantitative-assessment-of-both-systolic-and-diastolic-right-ventricular-morphology-and-function-by-conventional-echocardiography-and-speckle-tracking-imaging-in-dogs-a-prospective-study-in-104-healthy-dogs
#14
Valérie Chetboul, Cécile Damoiseaux, Hervé P Lefebvre, Didier Concordet, Loic Desquilbet, Vassiliki Gouni, Camille Poissonnier, Jean-Louis Pouchelon, Renaud Tissier
Our aim was (1) to determine the within-day and between-day variability of several indices of systolic and diastolic right ventricular (RV) function using conventional echocardiography and speckle tracking echocardiography (STE) (Study 1), (2) to quantify these variables in a large healthy canine population (n=104) with Doppler-derived estimated systolic pulmonary arterial pressure (SPAP) and left ventricular (LV) function, and (3) to establish the corresponding reference intervals (Study 2). For both studies, RV variables included tricuspid annular plane systolic excursion (TAPSE), fractional area change (RFAC), STE longitudinal systolic strain (StS) of the RV free wall (RVFW) and of the entire RV (i...
July 20, 2018: Journal of Veterinary Science
https://www.readbyqxmd.com/read/30041287/evidence-of-two-genetically-different-lymphotropic-herpesvirus-present-among-red-deer-cervus-elaphus-sambar-rusa-unicolor-and-milu-elaphurus-davidianus-herds-in-china
#15
Hongwei Zhu, Huitao Liu, Xin Yu, Jianlong Zhang, Linlin Jiang, Guozhong Chen, Zhibin Feng, Youzhi Li, Tao Feng, Xingxiao Zhang
Herpesvirus infections in Cervidae is a serious threat affecting some deer species worldwide. In our attempt to identify malignant catarrhal fever associated herpesviruses in deer herds, ten gammaherpesviral DNA fragments were identified in five species of deer herds in China by using a pan-herpesvirus PCR assay targeting viral DNA polymerase (DPOL). Notably, a novel gamma-2 herpesvirus in sambar ( Rusa unicolor ) was identified, which shows close relationship with Fallow deer-LHV, while the others are phylogenetically grouped together with Elk-LHV...
July 20, 2018: Journal of Veterinary Science
https://www.readbyqxmd.com/read/30041286/usefulness-of-transthoracic-lung-ultrasound-for-the-diagnosis-of-mild-pneumothorax
#16
Tae Sung Hwang, Young Min Yoon, Dong In Jung, Seong Chan Yeon, Hee Chun Lee
The aim of present study was to investigate the diagnostic accuracy of ultrasonography features for the detection of mild pneumothorax using CT in dogs. Nine adult healthy beagles were included in the study. The thoracic tube was inserted into pleural space at the left thoracic wall. All dogs underwent the examinations in the order of CT, lung ultrasound, and radiography examinations before the infusion of room air into the pleural space. Typically, 2, 3 (total 5 ml/kg), and 5 ml/kg (total 10 ml/kg) of room air was sequentially infused into the pleural space and CT, lung ultrasound, and radiography examinations were performed, respectively...
July 20, 2018: Journal of Veterinary Science
https://www.readbyqxmd.com/read/30041285/the-detection-of-progressive-and-regressive-phase-and-line-1-retrotransposon-in-transfected-dogs-with-transmissible-venereal-tumor-during-chemotherapy
#17
Sevil Atalay Vural, Rifki Haziroglu, Mehmet Rifat Vural, Ibrahim Mert Polat, Arda Selin Tunc
Canine transmissible venereal tumor (CTVT) is a tumor that commonly occurs in genital and extragenital sites of both genders. Long Interspersed Nuclear Elements (LINE-1) retrotransposon has the pivotal role in allogenic transfection amongst uncontrolled dog population. In the study, it is aimed to evaluate pathomorphological, immunohistochemical and in-situ-PCR (Polymerase Chain Reaction) of CTVT (n:18) in transfected dogs during chemotherapy. Immunohistochemically, the phases of the tumor were investigated using specific markers (CD3, CD4, CD8, CD79, TGF-β) and amplified specific sequence of TVT LINE-1 retrotransposon by in-situ-PCR method...
July 20, 2018: Journal of Veterinary Science
https://www.readbyqxmd.com/read/29695146/isolation-and-characterization-of-korean-porcine-deltacoronavirus-strain-knu16-07
#18
Guehwan Jang, Seong-Hee Kim, Yoo Jin Lee, Seungjoon Kim, Du Sik Lee, Kyoung-Ki Lee, Changhee Lee
Porcine deltacoronavirus (PDCoV) has emerged in several pig-raising countries and has been a causative pathogen associated with diarrheal diseases in South Korea since 2014. In the present study, we were able to isolate and cultivate a Korean PDCoV strain (KNU16-07) in cell culture and investigate its pathogenicity. PDCoV-inoculated piglets showed watery diarrhea accompanied by acute enteritis in the natural host. Sequencing analysis demonstrated the genetic stability of KNU16-07 for at least thirty serial passages...
July 31, 2018: Journal of Veterinary Science
https://www.readbyqxmd.com/read/29695145/sciatic-nerve-leachate-of-cattle-causes-neuronal-differentiation-of-pc12-cells-via-erk1-2-signaling-pathway
#19
Ziqiang Zhang, Yumei Liu, Xuemin Zhu, Lan Wei, Jiamin Zhu, Ke Shi, Guotao Wang, Li Pan
Previous studies have shown that the sciatic nerve has neurotrophic activity, and nerve regeneration, differentiation, and axon outgrowth can be modulated by different sciatic nerve preparations. However, numerous animals may have to be sacrificed to obtain enough sciatic nerves to make a sciatic nerve preparation. Some studies have demonstrated that the role of sciatic nerve preparations in neural differentiation depends on the neurotrophins that Schwann cells secrete, and these factors are highly conserved among different species...
July 31, 2018: Journal of Veterinary Science
https://www.readbyqxmd.com/read/29695143/transient-forebrain-ischemia-induces-impairment-in-cognitive-performance-prior-to-extensive-neuronal-cell-death-in-mongolian-gerbil-meriones-unguiculatus
#20
Tomohiro Kondo, Suguru Yoshida, Hiroaki Nagai, Ai Takeshita, Masaki Mino, Hiroshi Morioka, Takayuki Nakajima, Ken Takeshi Kusakabe, Toshiya Okada
In Mongolian gerbils, bilateral common carotid artery occlusion (BCCAO) for several minutes induces ischemia, due to an incomplete circle of Willis, resulting in delayed neuronal cell death in the Cornet d'Ammon 1 (CA1) region of the hippocampus. Neuronal cell death in the hippocampus and changes in behavior were examined after BCCAO was performed for 5 min in the gerbils. One day after BCCAO, the pyramidal neurons of the CA1 region of the hippocampus showed degenerative changes (clumped chromatin in nuclei)...
July 31, 2018: Journal of Veterinary Science
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