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Journal of Veterinary Science

Preeti Kumari Chaudhary, Soochong Kim
Platelet activation plays a major role in hemostasis and thrombosis. Various agonists including ADP and thrombin interact with GPCRs which transduce signals from the cell surface to intracellular effectors through various G proteins. Recent studies have elucidated the role of these receptors and their corresponding G proteins in the regulation of events involved in platelet activation. However, these agonists-induced platelet activation in companion animals is poorly understood. This study was designed to characterize the platelet response to various agonists in dog platelets...
December 4, 2018: Journal of Veterinary Science
Yo-Han Kim, Doo Kim
A total of 123 Coxiella burnetii strains detected in cattle from a nationwide survey in Korea were classified into the genomic groups I (0.8%), II (13.8%), III (11.5%), IV (63.1%), and V (5.4%). Acute Q-fever strain (groups I, II, and III) was 27.6%, and chronic strain (groups IV and V) was 72.4% of the total. A dairy farm, both types were detected, showed 6.7 times higher detection rate than another farm where only chronic Q-fever strains were detected. Both acute and chronic Q-fever strains are widespread in Korea, and it could represent an active transmission source to livestock and humans...
December 4, 2018: Journal of Veterinary Science
Viviana Manzulli, Antonio Fasanella, Antonio Parisi, Luigina Serrecchia, Adelia Donatiello, Valeria Rondinone, Marta Caruso, Sabine Zange, Alina Tscherne, Nicola Decaro, Carmine Pedarra, Domenico Galante
Anthrax, caused by Bacillus anthracis , is a non-contagious infectious disease that affects a wide range of animal species (primarily ruminants), as well as humans. Due to the often fatal outcome in human cases, the quick administration of definitely effective antimicrobials is crucial either as prophylaxis or for the therapy of clinical cases. In this study, a total of 110 B. anthracis strains, temporally, geographically and genetically different, isolated during anthrax outbreaks in Italy from 1984 to 2017, were screened for their susceptibility towards 16 clinically relevant antimicrobial agents using the broth microdilution method...
December 4, 2018: Journal of Veterinary Science
Chang-Min Lee, Doo-Won Song, Woong-Bin Ro, Min-Hee Kang, Hee-Myung Park
Genome-wide association study (GWAS) is a powerful tool for identifying the genetic causes of various diseases. This study conducted to identify genomic variation in Maltese dog genomes associated with degenerative mitral valve disease (DMVD) development and evaluate the association of each biological condition with DMVD in Maltese dogs. DNA was extracted from blood samples obtained from 48 Maltese dogs (32 with DMVD and 16 controls). Genome-wide single nucleotide polymorphism (SNP) genotyping was performed...
December 4, 2018: Journal of Veterinary Science
Hayoung Yang, Woosun Kim, Junwoo Bae, Hyunwoo Kim, Sangki Kim, Jihye Choi, Jinho Park, Dong-In Jung, Hongbum Koh, DoHyeon Yu
White blood cells (WBCs) and storage period are the main factors of transfusion reactions. In the present study, cytokine/chemokine concentrations after leukoreduction (LR) and irradiation (IR) in stored canine whole blood were measured. Red blood cell (RBC) storage lesion caused by IR and LR were also compared. Blood samples from 10 healthy Beagles were divided into four groups (no treatment, LR, IR, and LR + IR). Leukocytes were removed by filtration in the LR group and gamma radiation (25Gy) was applied in the IR group...
December 4, 2018: Journal of Veterinary Science
Stefan Vilcek, Slavomira Salamunova, Anna Jackova
Astroviruses were widely detected in pigs but their detection in wild boars is rather sporadic. In this study astroviruses were detected in organ homogenates of wild boars by a nested RT-PCR and the typing was carried out by phylogenetic analysis. Overall, 30/200 (15%) homogenates were positive for astroviruses. Genetic typing revealed that of 13 amplicons analysed, 8 were typed as porcine astrovirus lineage 2 (PAstV-2), and 2 as lineage 4 (PAstV-4). Two identical sequences were grouped with chicken astrovirus, and 1 sequence belonged to bat astrovirus...
November 26, 2018: Journal of Veterinary Science
Hyo-Kyung Bae, Bae-Dong Jung, Seunghyung Lee, Choon-Keun Park, Boo-Keun Yang, Hee-Tae Cheong
The objective of this study was to examine effects of spontaneous adipocyte generation on osteogenic differentiation of porcine skin-derived stem cells (pSSCs). Correlation between osteogenic differentiation and adipocyte differentiation induced by osteocyte induction culture was determined using different cell lines. Osteogenic differentiation efficiency of pSSCs was then analyzed by controlling the expression of adipocyte-specific transcription factors during osteogenic induction culture. Among four cell lines, pSSCs-II had the lowest level of lipid droplet but the highest calcium content ( P < 0...
November 26, 2018: Journal of Veterinary Science
Dimas Arya Abdillah, Setyawan Emn, Min Jung Kim, Hyun Ju Oh, Seok Hee Lee, Kihae Ra, Byeong Chun Lee
The objectives of this study were to analyze the protective effects of OptiprepTM (iodixanol) on dog spermatozoa during cryopreservation. The optimal concentration of OptiprepTM , 2.5%, was determined using fresh spermatozoa, and applied for the following experiment. The 2.5% OptiprepTM group showed significantly increased sperm motility than the control (P < 0.05). Lower mitochondrial ROS modulator ( ROMO1 ) and pro-apoptotic gene ( BAX ), together with higher expression of protamine-2 ( PRM2 ), protamine-3 ( PRM3 ), anti-apoptotic gene ( BCL2 ) and sperm acrosome associated-3 ( SPACA3 ), were expressed in the iodixanol treated group...
November 26, 2018: Journal of Veterinary Science
Jun Bong Lee, Se-Kye Kim, Seon Mi Wi, Young-Jae Cho, Tae-Wook Hahn, Jae-Yon Yu, Sungsun Kim, Sahyun Hong, Jonghyun Kim, Jang Won Yoon
Sequence type (ST) 33 of STEC O91:H14 has been proposed as a potential domestic clone of Shiga toxin-producing Escherichia coli (STEC) in Korea because of its high prevalence among human patients with mild diarrhea or asymptomatic carriers. Here, the clonal diversity of 17 STEC O91:H14 isolates of ST33 during 2003-2014 was analyzed by pulsed-field gel electrophoresis, including 14 isolates from human patients and 3 from retail meats. Their virulence characteristics, acid resistance, and antimicrobial susceptibility were also determined...
November 26, 2018: Journal of Veterinary Science
Seonmi Kang, Manbok Jeong, Kangmoon Seo
An 8-year-old Shih Tzu, a 5-year-old Maltese, and a 10-year-old Maltese presented with conjunctival hyperemia and peripheral corneal edema. Severe conjunctival thickening with varying degrees of corneal extension were observed. Cytological examination showed many large lymphocytes with malignant changes in the conjunctiva and were consistent with findings in fine-needle aspiration samples taken from the regional lymph nodes. They were diagnosed as having stage V multicentric lymphoma. When conjunctival thickening is observed in canine patients with multicentric lymphoma, conjunctival metastasis with infiltration of neoplastic lymphoid cells should be included in the differential diagnosis...
November 26, 2018: Journal of Veterinary Science
Nga Thi Thu Tham, So-Ryeon Hwang, Ji-Hyun Bang, Hee Yi, Young-Il Park, Seok-Jin Kang, Hwan-Goo Kang, Yong-Sang Kim, Hyun-Ok Ku
In vitro prediction of hepatotoxicity can enhance the performance of non-clinical animal testing for identifying the hazards of chemicals. Here, we assessed high-content analysis (HCA) using multi-parameter cell-based assays as an in vitro hepatotoxicity testing model using various hepatotoxicants and human hepatocytes such as HepG2 cells and human primary hepatocytes (hPHs). Both types of hepatocyte were exposed to multiple doses of ten hepatotoxicants associated with liver injury whose mechanisms of action are known...
November 26, 2018: Journal of Veterinary Science
Kyung-Hyo Do, Jae-Won Byun, Wan-Kyu Lee
To diagnose the colibacillosis, the detection of O-serogroups and virulence genes has been recommended worldwide. The prevalence of the viruence factors can fluctuate over time. The objectives of this the present study were to determine the prevalence of O-serogroups, virulence genes, and F18 subtypes among pathogenic Escherichia ( E. ) coli isolated from weaned piglets with diarrhea in Korea. Between 2008 and 2016, 362 E. coli were isolated from weaned piglets with diarrhea. Hemolysis was determined in blood agar, and O-serogroups were identified using the slide agglutination technique...
November 26, 2018: Journal of Veterinary Science
Jih-Jong Lee, Han-You Lin, Chun-An Chen, Chen-Si Lin, Lih-Chiann Wang
Canine MDR1 gene mutations produce the translated P-glycoprotein, an active drug efflux transporter, dysfunction or over-expression. The 4-base deletion of MDR1 gene at nucleotide position 230 (nt230[del4]) in exon 4 makes P-glycoprotein lose function, leading to drug accumulation and toxicity. The G-allele of the c.-6-180T>G variation in intron 1 of MDR1 gene (SNP 180) causes P-glycoprotein over-expression, making epileptic dogs resistant to phenobarbital treatment. Both of these mutations were reported to concentrate in collies...
November 26, 2018: Journal of Veterinary Science
Jeonghyeon Moon, Sangho Roh
Somatic cell nuclear transfer (SCNT) has various abilities of application in research, as well as the medical field and industry. However, the efficiency of SCNT is very low because the accurate mechanism of SCNT murine embryo development is still unknown. In previous studies, Polo-like kinase 1 (Plk1) has been found to be a crucial element in cell division including centrosome maturation, cytokinesis and spindle formation, and, in general, the developmental rate of SCNT murine embryos is lower than in vivo counterpart...
November 26, 2018: Journal of Veterinary Science
Yang-Chang Tu, Kuang-Yu Chen, Chung-Kung Chen, Ming-Chu Cheng, Shu-Hwae Lee, Ivan-Chen Cheng
Monoclonal antibody (MAb) is widely applied in disease diagnosis. Here we report a MAb, 1 WF-4, against Influenza A virus nucleoprotein (NP), its broad response with Influenza A virus , and its application in immunohistochemistry (IHC) assay. WF-4 was screened by immunofluorescence assay (IFA). The results showed that its reactivity with baculovirus expressed full-length recombinant NP (rNP) in western blot (WB) and applicability in IHC. Fifteen Influenza A virus reference subtypes, H1 to H15, infected chicken embryonated chorioallantoic membranes (CAM) fixed by formalin, were all detectable in WF-4 based IHC...
November 26, 2018: Journal of Veterinary Science
Chenxi Shi, Minmin Li, Ishfaq Muhammad, Xin Ma, Yicong Chang, Rui Li, Changwen Li, Jingshan He, Fangping Liu
Bacterial biofilms have been demonstrated to be closely related to clinical infections and contribute to drug resistance. Berberine, which is the main component of Coptis chinensis , has been reported to have efficient antibacterial activity. This study aimed to investigate the potential effect of a combination of berberine with ciprofloxacin (CIP) to inhibit Salmonella biofilm formation and its effect on expressions of related genes ( rpoE , luxS , and ompR ). The fractional inhibitory concentration (FIC) index of the combination of berberine with CIP is 0...
November 30, 2018: Journal of Veterinary Science
Inês Tenreiro Tavares, Ramón R Barreno, José P Sales-Luís, Carlo G Vaudano
An elective laparoscopic ovariectomy on a healthy dog revealed a cystic structure in the left ovary. The surgical procedure was successful. Histopathological examination showed the presence of a teratoma adjacent to the ovary. To the best of the authors' knowledge, this is the first reported case of an ovarian teratoma removed by laparoscopic ovariectomy in a dog using a multiport laparoscopic ovariectomy technique.
October 8, 2018: Journal of Veterinary Science
In-Ohk Ouh, Seyeon Park, Ju-Yeon Lee, Jae Young Song, In-Soo Cho, Hye-Ryung Kim, Choi-Kyu Park
Porcine parvovirus 7 (PPV7) was first detected in Korean pig farms in 2017. The detection rate of PPV7 DNA was 24.0% (30/125) in aborted pig fetuses and 74.9% (262/350) in finishing pigs, suggesting that PPV7 is circulated in Korean domestic pig farms. Phylogenetic analysis based on VP amino acid sequences demonstrated that the nine Korean strains (PPV-KA1-3 and PPV-KF1-6) were closely related to that of previously reported US and Chinese PPV7 strains. In addition, the Korean strains have genetic diversity with insertion or deletion mutations...
October 8, 2018: Journal of Veterinary Science
Tingting Zhao, Xiaojuan Shen, Chang Dai, Li Cui
Several studies have reported the effect of absorption of procyanidins and their contribution to small intestine. However, the differences of dietary interventions of procyanidins compared with antibiotic feeding in pigs are rare reported. After 16S rRNA gene Illumina MiSeq sequencing, we found that procyanidin administration for 2 months (procyanidin 1 group) or continuously antibiotics feeding for 1 month and then procyanidin for 1 month (procyanidin 2 group) could increase the number of OTUs, Chao 1 and ACE index compared with antibiotic administration for 2 months (antibiotic group)...
October 8, 2018: Journal of Veterinary Science
Joo-Hyung Choi, Kwiwan Jeong, Su-Mi Kim, Mi-Kyeong Ko, Su-Hwa You, Young S Lyoo, Byounghan Kim, Jin-Mo Ku, Jong-Hyeon Park
Foot-and-mouth disease (FMD) is a highly contagious viral vesicular disease of cloven hoofed animals caused by the foot-and-mouth disease virus (FMDV), which is one of the highly variable RNA viruses. In many countries, vaccines are used for the prevention of FMD. However, because there is no protection against FMD immediately after vaccination, research and development on antiviral agents is being conducted to induce protection until immunological competence is produced. Until now, no cases of the use of an instantly effective antiviral agent in the field for treatment or prevention have been reported...
October 8, 2018: Journal of Veterinary Science
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