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Journal of Veterinary Science

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https://www.readbyqxmd.com/read/28057911/guanosine-5-monophosphate-chelated-calcium-and-iron-feed-additives-maintains-egg-production-and-prevents-salmonella-gallinarum-in-experimentally-infected-layers
#1
Hye-Ji Noh, HeeKyong Kim, Su Jeong Heo, Hyang Hyun Cho, Hong Bum Koh
We evaluated the effects of guanosine 5'-monophosphate (GMP) -chelated calcium and iron (CaFe-GMP) on the health and egg quality in layers experimentally infected with Salmonella Gallinarum. In this study, CaFe-GMP feed additives was fed with commercial layer feed to layer over four weeks and all were inoculated with Salmonella Gallinarum. Body weight, mortality, clinical symptoms, and poultry production including feed intake, egg production, egg loss, and feed conversion rate were observed and Salmonella Gallinarum was re-isolated from the liver, spleen, and cecum of the layers...
January 4, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28057910/arthroscopic-detection-of-medial-meniscal-injury-in-small-breed-dogs-with-the-use-of-a-joint-distractor
#2
Joo-Ho Kim, Su-Young Heo, Hae-Beom Lee
The goal of the present study was to evaluate the diagnostic value of a joint distractor in arthroscopy in small-breed dogs. Sixty stifle joints, which were collected from thirty cadavers, were used in this study. To simulate different injuries, no medial meniscal tear, a full-thickness vertical longitudinal tear, a partial-thickness vertical longitudinal tear, full- and partial-thickness vertical longitudinal tears, or a peripheral detachment were made on the caudal horn of the medial meniscus of each stifle joint along with cranial cruciate ligament (CrCL) rupture...
January 4, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28057909/fluoroscopic-characteristics-of-tracheal-collapse-and-cervical-lung-herniation-in-dogs-222-cases-2012-2015
#3
Jeosoon Lee, Sookyung Yun, Inhyung Lee, Mincheol Choi, Junghee Yoon
This retrospective study was performed in 222 dogs suspected to have tracheal collapse (TC) from January 1, 2012 to December 31, 2015. Fluoroscopy and radiographic images with medical records were evaluated to investigate the fluoroscopic characteristics of TC and cervical lung herniation (CLH), and determine their relationship to various influencing factors. Fluoroscopically, grade of TC, existence of tracheal kinking, location of CLH (right or left side), and grade of CLH were evaluated. One hundred ninety nine dogs (89...
January 4, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28057908/clinical-application-of-quantitative-computed-tomography-in-osteogenesis-imperfecta-suspected-cat
#4
Sungjun Won, Woo-Jo Chung, Junghee Yoon
One year-old male Persian cat was presented with multiple fractures without known traumatic history. Marked decrease of bone radiopacity and thin cortices of all long bones were identified in radiography. Tentative diagnosis was osteogenesis imperfecta, which is congenital disorder characterized with fragile bone. To identify bone material density (BMD), quantitative computed tomography (QCT) was performed. With QCT, mean trabecular BMD of vertebral bodies was 149.9 ± 86.5 mg/cm³. After bisphosphonate therapy, BMD of same site was 218...
January 4, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28057907/molecular-cloning-and-characterization-of-the-porcine-ribosomal-protein-l21
#5
Wu-Sheng Sun, Ju-Lan Chun, Dong-Hwan Kim, Jin-Seop Ahn, Min-Kyu Kim, In-Sul Hwang, Dae-Jin Kwon, Seong-Soo Hwang, Jeong-Woong Lee
Ribosomal protein L21 (RPL21) is a structural component of the 60S subunit of the eukaryotic ribosome. This protein plays an important role in protein synthesis and the occurrence of hereditary diseases. Pig is a common laboratory model, however, to the best of our knowledge, its RPL21 gene has not been cloned to date. In this study, we cloned and identified the full-length sequence of the pig RPL21 gene for the first time. Then we studied its expression pattern and function by overexpression or knockdown approach...
January 4, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28057906/analysis-of-porcine-rotavirus-a-enterotoxin-gene-for-structure-function-relationship
#6
Sharad Saurabh, Shubhankar Sircar, Jobin Jose Kattoor, Souvik Ghosh, N Kobayashi, Krisztian Banyai, O R Vinod Kumar, Ujjawal De, Nihar Ranjan Sahoo, Kuldeep Dhama, Yashpal Singh Malik
Rotavirus (RV) infected piglets are presumed to be the latent sources of heterologous RV infection to humans and other animals. In RVs, non-structural protein 4 (NSP4) is the major virulence factor with pleiotropic properties. In this study, we analyzed nsp4 gene from porcine RVs, isolated from diarrheic and non-diarrheic cases, at different levels of protein folding to explore any correlation with their diarrhea inducing capabilities and evolution of nsp4 in porcine population. The full length nsp4 genes were amplified, cloned, sequenced and analyzed for antigenic epitopes, RotaC classification, homology, genetic relationship, modeling of NSP4 protein and prediction for post translational modification...
January 4, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28057905/development-of-an-immunochromatographic-strip-for-the-detection-of-antibodies-against-porcine-reproductive-and-respiratory-syndrome-virus
#7
Huawei Li, Jifei Yang, Dengke Bao, Jie Hou, Yubao Zhi, Yanyan Yang, Pengchao Ji, Enmin Zhou, Songlin Qiao, Gaiping Zhang
A simple and rapid immunochromatographic test strip using a colloidal gold-labeled recombinant Nsp7 antigen probe was successfully developed for the detection of anti-PRRSV antibodies in swine. Recombinant Nsp7 protein of PRRSV labeled with colloidal gold was dispensed on a conjugate pad as the detector. Staphylococcal protein A (SPA) and purified porcine anti-Nsp7 antibodies were blotted on a nitrocellulose membrane for the test and control lines, respectively. A comparison between the new assay and standard diagnostic tests enzyme-linked immunosorbent assays (ELISA) and immunoperoxidase monolayer assay (IPMA) was also performed...
January 4, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28057904/a-serine-threonine-phosphatase-1-of-streptococcus-suis-type-2-is-an-important-virulence-factor
#8
Lihua Fang, Pengcheng Fan, Yunkai Yang, Jingjing Zhou, Hongxia Shen, Weihuan Fang
Streptococcus suis is regarded as one of the major pathogens of pigs while S. suis type 2 (SS2) is considered to be a zoonotic bacterium for its ability to cause meningitis and streptococcal toxic shock-like syndrome in humans. Many bacterial species contain genes encoding serine/threonine protein phosphatases (STP) responsible for dephosphorylation of their substrates in a single reaction step. This study was aimed to investigate the role of stp1 in pathogenesis of SS2. An isogenic stp1 mutant (Δstp1) was constructed from the SS2 strain ZJ081101...
January 4, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28057903/reactive-oxygen-species-mediated-unfolded-protein-response-pathways-in-pre-implantation-embryo
#9
REVIEW
Ihsan Ali, Syed Zahid Ali Shah, Jin Yi, Li Zhong-Shu, Obaidullah, Fang Nan-Zhu
The excessive production of reactive oxygen species (ROS) and endoplasmic reticulum (ER) stress mediated responses are critical to embryonic development in the challenging in-vitro environment. ROS production increases during early embryonic development with the increased protein requirement for cell survival and growth. The endoplasmic reticulum (ER) is a multifunctional cellular organelle responsible for protein folding, modification, and cellular homeostasis. ER stress is activated by a variety of factors including ROS, leading to activation of the adaptive unfolded protein response (UPR), which restores homeostasis...
January 4, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28057902/are-pulmonary-haemostasis-and-fibrinolysis-out-of-balance-in-equine-chronic-pneumopathies
#10
Ann Kristin Barton, Caroline Wirth, Angelika Bondzio, Ralf Einspanier, Heidrun Gehlen
Results of clinical examination, bronchoalveolar lavage fluid (BALF) cytology, acute phase proteins and markers of pulmonary haemostasisand fibrinolysis (fibrinogen, serum amyloid A, D-dimers) were compared between controls and horses affected by respiratory disease. Using a clinical scoring system, 58 horses were classified as free of respiratory disease (controls, n=15), recurrent airway obstruction (RAO, n=18), inflammatory airway disease (IAD, n=14) or chronic interstitial pneumopathy (CIP, n=11). No significant differences were found in fibrinogen concentration, but descriptive data analysis showed a trend towards lower values for controls (median 0...
January 4, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28057901/genetic-diversity-and-phylogenetic-analysis-of-porcine-reproductive-and-respiratory-syndrome-virus-in-southern-china-from-2007-to-2014
#11
Qiwen Wu, Zhili Li, Guanqun Zhang, Jianqiang Niu, Xiduo Zeng, Baoli Sun, Jingyun Ma
Porcine reproductive and respiratory syndrome virus (PRRSV) has a high degree of genetic variation. Here, we characterized the genetic variation and evolutionary relationships among circulating PRRSV strains in Southern China. In this study, we analyzed 29 NSP2 strains and 150 ORF5 strains from clinical samples collected in Southern China during 2007-2014. The alignment results showed that the nucleotide identities of the two genes among these strains were 80.5-99.7% and 80.9-100%, respectively. Phylogenetic analysis based on NSP2 gene showed that high pathogenic PRRSV were still the dominant virus in Southern China from 2013 to 2014...
January 4, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28057900/evaluation-of-the-ureteral-jet-in-dogs-using-doppler-ultrasonography
#12
Hyunjung Oh, Seongsoo Kim, Suyeon Kim, Jeosoon Lee, Sookyung Yun, Junghee Yoon, Joohyun Jung, Mincheol Choi
Ureteral jets are caused by the forceful ejection of urine from the vesicoureteral junction into the urinary bladder. We aimed to identify distinct ureteral jets in dogs by using color Doppler ultrasonography (US), provide insight into ureteral obstruction, and facilitate the study of urodynamics and vesicoureteric sphincter function using pulsed Doppler US. Color Doppler US was applied to detect urinary flow from the right ureteral orifice in eight healthy beagles. Under anesthesia, 0.9% saline (2.5 ml/kg/h) and furosemide (0...
January 4, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28057899/retrospective-evaluation-of-circulating-thyroid-hormones-in-critically-ill-dogs-with-systemic-inflammatory-response-syndrome
#13
Massimo Giunti, Roberta Troia, Mara Battilani, Luciana Giardino, Francesco Dondi, Giulia Andreani, Federico Fracassi
Critical illness can be associated with transient alterations in circulating thyroid hormones concentration indicating Non-Thyroidal Illness (NTI). NTI is well described in humans; literature concerning its occurrence and prognostic significance in dogs remains limited. The aim of this retrospective study was to assess the occurrence of NTI in a population of dogs with Systemic Inflammatory Response Syndrome (SIRS), and to investigate its association with disease severity (Apple Fast Score). A total of 41 SIRS dogs (acute pancreatitis n=10; parvovirus n=22; septic peritonitis n=9) were included, and divided according to SIRS origin (non-septic SIRS: acute pancreatitis; septic-SIRS: parvovirus and septic peritonitis) and final outcome (survivors, n=37; non-survivors, n=4)...
January 4, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28057898/a-retrospective-study-of-degenerative-mitral-valve-disease-in-small-breed-dogs-survival-and-prognostic-variables
#14
Hyun-Tae Kim, Sei-Myoung Han, Woo-Jin Song, Boeun Kim, Mincheol Choi, Junghee Yoon, Hwa-Young Youn
Small-breed (n= 168 dogs; weight < 15 kg) diagnosed with MMVD based on a routine clinical examination, radiology, an electrocardiogram, and echocardiography at the Seoul National University Veterinary Medical Teaching Hospital were included. The survival periods were ascertained and a significant difference was observed in the survival rates between the International Small Animal Cardiac Health Council classes. The mean follow-up period was 14.3 ± 12.1 months. Univariate analysis revealed that dyspnea, pulmonary edema, and vertebral heart score were significantly associated with the survival times (P < 0...
January 4, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/27994210/mycobacterium-vaccae-induces-a-strong-th1-response-that-subsequently-declines-in-c57bl-6-mice
#15
Lijiao Zhang, Yanlong Jiang, Ziyin Cui, Wentao Yang, Limin Yue, Yingcong Ma, Shaohua Shi, Chunfang Wang, Chunfeng Wang, Aidong Qian
Mycobacterium (M.) vaccae is a fast-growing species of saprophytic bacteria that is widely distributed. To understand the host immune responses induced by M. vaccae isolated from bovine submaxillary lymph nodes, C57BL/6 mice were infected with reference strain M. bovis Bacillus Calmette-Guérin (BCG) and isolated M. vaccae using intraperitoneal injections. Comparison of the bacterial replication and organ pathology between M. vaccae and M. bovis BCG revealed that M. vaccae was more malignant than M. bovis in mice...
December 30, 2016: Journal of Veterinary Science
https://www.readbyqxmd.com/read/27456779/epizootiological-characteristics-of-viable-bacteria-and-fungi-in-indoor-air-from-porcine-chicken-or-bovine-husbandry-confinement-buildings
#16
Katharine Roque, Gyeong-Dong Lim, Ji-Hoon Jo, Kyung-Min Shin, Eun-Seob Song, Ravi Gautam, Chang-Yul Kim, Kyungsuk Lee, Seungwon Shin, Han-Sang Yoo, Yong Heo, Hyoung-Ah Kim
Microorganisms found in bioaerosols from animal confinement buildings not only foster the risk of spreading diseases among livestock buildings, but also pose health hazards to farm workers and nearby residents. This study identified the various microorganisms present in the air of swine, chicken, and cattle farms with different kinds of ventilation conditions in Korea. Microbial air samples were collected onto Petri dishes with bacterial or fungal growth media using a cascade impactor. Endotoxin levels in total dust were determined by the limulus amebocyte lysate kinetic QCL method...
December 30, 2016: Journal of Veterinary Science
https://www.readbyqxmd.com/read/27456778/genomic-diversity-of-the-avian-leukosis-virus-subgroup-j-gp85-gene-in-different-organs-of-an-infected-chicken
#17
Fanfeng Meng, Xue Li, Jian Fang, Yalong Gao, Lilong Zhu, Guiju Xing, Fu Tian, Yali Gao, Xuan Dong, Shuang Chang, Peng Zhao, Zhizhong Cui, Zhihao Liu
The genomic diversity of Avian leukosis virus subgroup J (ALV-J) was investigated in an experimentally infected chicken. ALV-J variants in tissues from four different organs of the same bird were re-isolated in DF-1 cells, and their gp85 gene was amplified and cloned. Ten clones from each organ were sequenced and compared with the original inoculum strain, NX0101. The minimum homology of each organ ranged from 96.7 to 97.6%, and the lowest homology between organs was only 94.9%, which was much lower than the 99...
December 30, 2016: Journal of Veterinary Science
https://www.readbyqxmd.com/read/27456776/molecular-and-phylogenetic-analysis-of-anaplasma-spp-in-sheep-and-goats-from-six-provinces-of-china
#18
Yan Zhang, Yali Lv, Feifei Zhang, Wenjing Zhang, Jinhong Wang, Yanyan Cui, Rongjun Wang, Fuchun Jian, Longxian Zhang, Changshen Ning
Members of the genus Anaplasma are important emerging tick-borne pathogens in both humans and animals in tropical and subtropical areas. Here, we investigated the presence of Anaplasma spp. in 621 sheep and 710 goats from six provinces of China. Polymerase chain reaction (PCR) and DNA sequencing were conducted to determine the prevalence of Anaplasma (A.) phagocytophilum, A. ovis and A. bovis targeting the 16S ribosomal RNA or the major surface protein 4 gene. PCR revealed Anaplasma in 39.0% (240/621) of sheep and 45...
December 30, 2016: Journal of Veterinary Science
https://www.readbyqxmd.com/read/27297425/canine-adipose-tissue-derived-mesenchymal-stem-cells-ameliorate-severe-acute-pancreatitis-by-regulating-t-cells-in-rats
#19
Hyun-Wook Kim, Woo-Jin Song, Qiang Li, Sei-Myoung Han, Kee-Ok Jeon, Sang-Chul Park, Min-Ok Ryu, Hyung-Kyu Chae, Kweon Kyeong, Hwa-Young Youn
Severe acute pancreatitis (SAP) is associated with systemic complications and high mortality rate in dogs. Mesenchymal stem cells (MSCs) have been investigated for their therapeutic potential in several inflammation models. In the present study, the effects of canine adipose tissue-derived (cAT)-MSCs in a rat model of SAP induced by retrograde injection of 3% sodium taurocholate solution into the pancreatic duct were investigated. cAT-MSCs labeled with dioctadecyl-3,3,3'-tetramethylindo-carbocyanine perchlorate (1 × 10⁷ cells/kg) were systemically administered to rats and pancreatic tissue was collected three days later for histopathological, quantitative real-time polymerase chain reaction, and immunocytochemical analyses...
December 30, 2016: Journal of Veterinary Science
https://www.readbyqxmd.com/read/27297422/toxicity-of-crude-and-detoxified-tityus-serrulatus-venom-in-anti-venom-producing-sheep
#20
Marina G Ferreira, Clara G Duarte, Maira S Oliveira, Karen L P Castro, Maílson S Teixeira, Lílian P G Reis, José A Zambrano, Evanguedes Kalapothakis, Ana Flávia R M Michel, Benito Soto-Blanco, Carlos Chávez-Olórtegui, Marília M Melo
Specific anti-venom used to treat scorpion envenomation is usually obtained from horses after hyperimmunization with crude scorpion venom. However, immunized animals often become ill because of the toxic effects of the immunogens used. This study was conducted to evaluate the toxic and immunogenic activities of crude and detoxified Tityus serrulatus (Ts) venom in sheep during the production of anti-scorpionic anti-venom. Sheep were categorized into three groups: G1, control, immunized with buffer only; G2, immunized with crude Ts venom; and G3, immunized with glutaraldehyde-detoxified Ts venom...
December 30, 2016: Journal of Veterinary Science
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