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Annals of Anatomy, Anatomischer Anzeiger: Official Organ of the Anatomische Gesellschaft

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https://www.readbyqxmd.com/read/29196235/morphological-features-of-the-anterior-talofibular-ligament-by-the-number-of-fiber-bundles
#1
Mutsuaki Edama, Ikuo Kageyama, Takanori Kikumoto, Masatoshi Nakamura, Wataru Ito, Emi Nakamura, Ryo Hirabayashi, Tomoya Takabayashi, Takuma Inai, Hideaki Onishi
The aims of this study have been to clarify differences in morphological features based on the number of fiber bundles in the anterior talofibular ligament (ATFL), and to investigate the relationship between the ATFL and the calcaneofibular ligament (CFL). This study used 81 legs from 43 cadavers. The ATFL was classified according to differences in the number of fiber bundles as: Type I, with one fiber bundle; Type II-a, with two fiber bundles that were incompletely separated; Type II-b, with two fiber bundles that were completely separated; and Type III, with three fiber bundles...
November 28, 2017: Annals of Anatomy, Anatomischer Anzeiger: Official Organ of the Anatomische Gesellschaft
https://www.readbyqxmd.com/read/29175126/immunolocalization-of-connective-tissue-growth-factor-transforming-growth-factor-beta1-and-phosphorylated-smad2-3-during-the-postnatal-tooth-development-and-formation-of-junctional-epithelium
#2
Shubo Li, Yihuai Pan
Connective tissue growth factor (CTGF) is a downstream mediator of transforming growth factor-beta 1 (TGF-β1) and TGF-β1-induced CTGF expression is regulated through SMAD pathway. However, there is no literature showing the expression of TGF-β1-SMAD2/3-CTGF signaling pathway during postnatal tooth development and the formation of junctional epithelium (JE). Hence, we aimed to analyze the localization of TGF-β1, CTGF and phosphorylated SMAD2/3 (p-SMAD2/3) in the developing postnatal rat molars. Wistar rats were killed at postnatal (PN) 0...
November 21, 2017: Annals of Anatomy, Anatomischer Anzeiger: Official Organ of the Anatomische Gesellschaft
https://www.readbyqxmd.com/read/29169842/anatomic-characterization-of-the-radial-and-ulnar-nutrient-arteries-in-humans
#3
Shota Kinose, Yosuke Kanaya, Yuto Kawasaki, Taro Okamura, Kota Kato, Tatsuo Sakai, Koichiro Ichimura
BACKGROUND: The localization of nutrient foramens has been extensively studied in humans and other vertebrate animals. However, accurate information on the origin and extraosseous course of the nutrient arteries in some types of long tubular bones is lacking. Terminologia Anatomica, the international standard on human anatomic terminology, lists the radial nutrient artery (RNA) and the ulnar nutrient artery (UNA) as branches of the radial and ulnar arteries, respectively. Anatomy textbooks published in both German- and English-speaking countries regard both the RNA and UNA as branches of the anterior interosseous artery...
November 20, 2017: Annals of Anatomy, Anatomischer Anzeiger: Official Organ of the Anatomische Gesellschaft
https://www.readbyqxmd.com/read/29169841/distribution-and-neurochemistry-of-porcine-urinary-bladder-projecting-sensory-neurons-in-subdomains-of-the-dorsal-root-ganglia-a-quantitative-analysis
#4
Anna Kozłowska, Anita Mikołajczyk, Mariusz Majewski
The aim of the present study has been to verify the inter- and intraganglionic distribution pattern of porcine urinary bladder-projecting (UBP) neurons localized in the sacral dorsal root ganglia (DRGs). The morphology and chemical phenotype of these cells have also been investigated. These neurons were visualized using the fluorescent tracer Fast Blue (FB) which was injected bilaterally into the urinary bladder wall of five juvenile female pigs. The intraganglionic distribution showed that small- and medium-sized FB+ perikarya were mainly located in the central (S3-S4) and periphero-central (S2) region of the ganglia, while large cells were heterogeneously distributed...
November 20, 2017: Annals of Anatomy, Anatomischer Anzeiger: Official Organ of the Anatomische Gesellschaft
https://www.readbyqxmd.com/read/29166622/morphological-characteristics-and-variations-of-the-human-quadratus-plantae-muscle
#5
Bettina Pretterklieber
The quadratus plantae (QP) is a highly variable structure. A number of partly inexact descriptions of this entity have been provided in textbooks of anatomy. Although several authors have examined the QP, its exact site of origin and type of insertion have hitherto not been specified. The aim of this study has been to provide detailed qualitative and quantitative data about the number of heads, points of origin, and type of insertion of the QP. The QP in both feet of 50 formalin-fixed specimens of body donors (25 men and women) were analyzed by gross anatomical dissection...
November 19, 2017: Annals of Anatomy, Anatomischer Anzeiger: Official Organ of the Anatomische Gesellschaft
https://www.readbyqxmd.com/read/29162481/dissociation-of-mono-and-co-culture-spheroids-into-single-cells-for-subsequent-flow-cytometric-analysis
#6
Ute Grässer, Monika Bubel, Daniela Sossong, Martin Oberringer, Tim Pohlemann, Wolfgang Metzger
BACKGROUND: Spheroids are considered to reflect the natural organization of cells better than 2D cell cultures, but their analysis by flow cytometry requires dissociation into single cells. METHODS: We established protocols for dissociation of mono- and co-culture spheroids consisting of human fibroblasts and human endothelial cells. Cell recovery rate and viability after dissociation were evaluated with hemocytometer and by flow cytometry. The diameter of cells and the amount of cell aggregates were quantified by Casy(®)-technology and the cellular composition was analyzed by flow cytometry...
November 18, 2017: Annals of Anatomy, Anatomischer Anzeiger: Official Organ of the Anatomische Gesellschaft
https://www.readbyqxmd.com/read/29146471/periodic-acid-schiff-pas-reaction-and-plastination-in-whole-body-slices-a-novel-technique-to-identify-fascial-tissue-structures
#7
Hanno Steinke, Dina Wiersbicki, Marie-Lynn Speckert, Claudia Merkwitz, Thomas Wolfskämpf, Benjamin Wolf
Since collagen rich fascial tissue is often very delicate and difficult to discern on native tissue slices, we have developed a method for staining full-body slices using the periodic acid-Schiff (PAS) reaction with subsequent plastination. Since the PAS reaction primarily stains carbohydrates, we could exploit the circumstance that different collagen types vary in carbohydrate content. Contrary to fasciae, tissues such as muscle, bone, nerves and blood vessels exhibit significantly less staining or remain unstained...
November 13, 2017: Annals of Anatomy, Anatomischer Anzeiger: Official Organ of the Anatomische Gesellschaft
https://www.readbyqxmd.com/read/29017852/an-augmented-reality-magic-mirror-as-additive-teaching-device-for-gross-anatomy
#8
Daniela Kugelmann, Leonard Stratmann, Nils Nühlen, Felix Bork, Saskia Hoffmann, Golbarg Samarbarksh, Anna Pferschy, Anna Maria von der Heide, Andreas Eimannsberger, Pascal Fallavollita, Nassir Navab, Jens Waschke
When preparing young medical students for clinical activity, it is indispensable to acquaint them with anatomical section images which enable them to use the clinical application of imaging methods. A new Augmented Reality Magic Mirror (AR MM) system, which provides the advantage of a novel, interactive learning tool in addition to a regular dissection course, was therefore tested and evaluated by 880 first-year medical students as part of the macroscopic anatomy course in 2015/16 at Ludwig-Maximilians-Universität (LMU) in Munich...
October 7, 2017: Annals of Anatomy, Anatomischer Anzeiger: Official Organ of the Anatomische Gesellschaft
https://www.readbyqxmd.com/read/28963046/introducing-a-simple-method-of-maxillary-sinus-volume-assessment-based-on-linear-dimensions
#9
Agnieszka Przystańska, Tomasz Kulczyk, Artur Rewekant, Alicja Sroka, Katarzyna Jończyk-Potoczna, Dorota Lorkiewicz-Muszyńska, Krzysztof Gawriołek, Agata Czajka-Jakubowska
Measuring sinus volume in a general practice clinic is a complex and time-consuming procedure, requiring experience in the use of radiological methods In the presented research, the automatically estimated maxillary sinus volume was compared with maxillary sinus volume assessed with mathematical formulas used to calculate the volume of spheres and pyramids. The starting point for the statistical analysis were specific measurements of the sinuses. We wanted to discover which geometric shape has the volume that is nearest to the automatically estimated volume...
September 28, 2017: Annals of Anatomy, Anatomischer Anzeiger: Official Organ of the Anatomische Gesellschaft
https://www.readbyqxmd.com/read/28963045/bodies-of-children-in-the-institute-of-anatomy-in-halle-saale-germany-between-1920-and-1945
#10
F Winter, D Stoevesandt, R Schultka, M Viebig, K Moeller, F Steger, H Kielstein
At the Institute of Anatomy and Cell Biology in Halle (Saale) 74 children's bodies of unknown historical provenance are being held in storage. The aim of this study was the evaluation of their identities, the circumstances of their acquisition, as well as the documentation of their individual characteristics. For these purposes, all bodies were comprehensively examined and photo-documented. Furthermore, CT-scans of 29 bodies were performed and information was collected from various local and national archives...
September 27, 2017: Annals of Anatomy, Anatomischer Anzeiger: Official Organ of the Anatomische Gesellschaft
https://www.readbyqxmd.com/read/28954211/calcium-binding-proteins-expression-in-the-septum-and-cingulate-cortex-of-the-adult-guinea-pig
#11
Beata Hermanowicz-Sobieraj, Krystyna Bogus-Nowakowska, Anna Robak
For the first time this study demonstrates the distribution pattern and expression of three neuroanatomical markers: calbindin D28k (CB), calretinin (CR) and parvalbumin (PV) in topographically connected brain regions - the septum (SE) and the cingulate cortex (CC). The co-existence among calcium-binding proteins (CaBPs) was also examined. The study was conducted on the adult guinea pig with the use of immunohistochemical and molecular biological techniques. Among the studied CaBPs, which occurred in both examined brain regions at transcript and protein levels, CB was the most expressed in the SE, while CR in the CC...
September 24, 2017: Annals of Anatomy, Anatomischer Anzeiger: Official Organ of the Anatomische Gesellschaft
https://www.readbyqxmd.com/read/28954210/gonial-angle-growth-patterns-according-to-age-and-gender
#12
Carolina Larrazabal-Moron, Juan A Sanchis-Gimeno
Currently there are controversial results about gender and age differences in human gonial angle values. In this context we aimed to ascertain the gender and age differences in the gonial angle values of young Caucasian Mediterranean subjects. We tested the hypothesis of a relation between the gonial angle values and the gender and age of the subjects by means of a prospective study involving 266 subjects. Panoramic radiographs (Cranex Novus(®), XMIND Novus(®) Soredex, France) were carried out in order to measure the gonial angle values...
September 24, 2017: Annals of Anatomy, Anatomischer Anzeiger: Official Organ of the Anatomische Gesellschaft
https://www.readbyqxmd.com/read/28954209/a-micro-computed-tomographic-micro-ct-analysis-of-the-root-canal-morphology-of-maxillary-third-molar-teeth
#13
Iwona M Tomaszewska, Bartosz Leszczyński, Andrzej Wróbel, Tomasz Gładysz, Hal F Duncan
INTRODUCTION: The aim of this study was to analyze the root canal morphology of maxillary third molars (MTMs) using micro-computed tomography (micro-CT). MATERIALS AND METHODS: Seventy-eight consecutively-extracted human MTMs were scanned using micro-CT (spatial resolution=13.68μm per pixel). Dedicated software (SkyScan(®)) was used to create virtual reconstructions and perform 3D-analysis. A range of anatomical features were assessed; externally (root number, length, fusion, curvature, apex), within the pulp chamber (distance between canal orifices, floor thickness) and within the root canal system (root canal number, classification, ramifications, isthmuses, apical constriction)...
September 24, 2017: Annals of Anatomy, Anatomischer Anzeiger: Official Organ of the Anatomische Gesellschaft
https://www.readbyqxmd.com/read/28954208/molecular-basis-of-dental-sensitivity-the-odontoblasts-are-multisensory-cells-and-express-multifunctional-ion-channels
#14
REVIEW
A Solé-Magdalena, M Martínez-Alonso, C A Coronado, L M Junquera, J Cobo, J A Vega
Odontoblasts are the dental pulp cells responsible for the formation of dentin. In addition, accumulating data strongly suggest that they can also function as sensory cells that mediate the early steps of mechanical, thermic, and chemical dental sensitivity. This assumption is based on the expression of different families of ion channels involved in various modalities of sensitivity and the release of putative neurotransmitters in response to odontoblast stimulation which are able to act on pulp sensory nerve fibers...
September 24, 2017: Annals of Anatomy, Anatomischer Anzeiger: Official Organ of the Anatomische Gesellschaft
https://www.readbyqxmd.com/read/28954207/effects-of-%C3%A3-tcp-scaffolds-on-neurogenic-and-osteogenic-differentiation-of-human-embryonic-stem-cells
#15
Premjit Arpornmaeklong, Michael J Pressler
Extracellular matrix (ECM) and adhesion molecules play crucial roles in regulating growth and differentiation of stem cells. The current study aimed to investigate the effects of beta-tricalcium phosphate (ß-TCP) scaffolds on differentiation and expression of ECM and adhesion molecules of human embryonic stem cells (hESCs). Undifferentiated hESCs were seeded on ß-TCP scaffolds and cell culture plates and cultured in growth and osteogenic medium for 21 days. Scanning electron microscopy (SEM) displayed adhesion and growth of hESCs on the porous ß-TCP scaffolds...
September 24, 2017: Annals of Anatomy, Anatomischer Anzeiger: Official Organ of the Anatomische Gesellschaft
https://www.readbyqxmd.com/read/28954206/phospholipase-c-zeta-parameters-in-sperm-from-polymorphic-teratozoospermic-men
#16
Nahid Azad, Hamid Nazarian, Marefat Ghaffari Novin, Reza Masteri Farahani, Abbas Piryaei, Mohammad Hassan Heidari
Teratozoospermia is a disorder associated with high abnormal sperm morphology which affects fertility in males. In recent years, it has been described that biomarker-based sperm quality evaluation can alleviate male infertility treatment. Phospholipase C zeta (PLCζ) is a sperm-specific factor which appears to be a predicting biomarker for fertilization potential of males. Following fertilization, PLCζ enters into oocyte cytoplasm and induces oocyte activation, a fundamental stage in initiation of embryo development...
September 24, 2017: Annals of Anatomy, Anatomischer Anzeiger: Official Organ of the Anatomische Gesellschaft
https://www.readbyqxmd.com/read/28935565/comparative-postnatal-histomorphogenesis-of-the-mandible-in-wild-and-laboratory-mice
#17
Jessica Martínez-Vargas, Cayetana Martinez-Maza, Francesc Muñoz-Muñoz, Nuria Medarde, Hayat Lamrous, María José López-Fuster, Jorge Cubo, Jacint Ventura
The coordinated activity of bone cells (i.e., osteoblasts and osteoclasts) during ontogeny underlies observed changes in bone growth rates (recorded in bone histology and bone microstructure) and bone remodeling patterns explaining the ontogenetic variation in bone size and shape. Histological cross-sections of the mandible in the C57BL/6J inbred mouse strain were recently examined in order to analyze the bone microstructure, as well as the directions and rates of bone growth according to the patterns of fluorescent labeling, with the aim of description of the early postnatal histomorphogenesis of this skeletal structure...
September 18, 2017: Annals of Anatomy, Anatomischer Anzeiger: Official Organ of the Anatomische Gesellschaft
https://www.readbyqxmd.com/read/28931468/platelet-released-growth-factors-inhibit-proliferation-of-primary-keratinocytes-in-vitro
#18
Andreas Bayer, Mersedeh Tohidnezhad, Rouven Berndt, Sebastian Lippross, Peter Behrendt, Tim Klüter, Thomas Pufe, Holger Jahr, Jochen Cremer, Franziska Rademacher, Maren Simanski, Regine Gläser, Jürgen Harder
Autologous thrombocyte concentrate lysates as platelet-released growth factors (PRGF) or Vivostat Platelet Rich Fibrin (PRF(®)) represent important tools in modern wound therapy, especially in the treatment of chronic, hard-to-heal or infected wounds. Nevertheless, underlying cellular and molecular mechanisms of the beneficial clinical effects of a local wound therapy with autologous thrombocyte concentrate lysates are poorly understood. Recently, we have demonstrated that PRGF induces antimicrobial peptides in primary keratinocytes and accelerates keratinocytes' differentiation...
September 18, 2017: Annals of Anatomy, Anatomischer Anzeiger: Official Organ of the Anatomische Gesellschaft
https://www.readbyqxmd.com/read/28823877/immunohistochemical-and-western-blot-analysis-suggest-that-the-soluble-forms-of-fgf1-2-and-fgfr1-2-sustain-tail-regeneration-in-the-lizard
#19
Lorenzo Alibardi
Fibroblast Growth Factors 1-2 (FGF1-2) stimulate tail regeneration in lizards and therefore the distribution of their receptors, FGFR1-2, in the regenerating tail of the lizard. Podarcis muralis has been studied using immunofluorescence and western blotting. Immunoreactive protein bands at 15-16kDa for FGF1-2 in addition to those at 50-65kDa are detected in the regenerating epidermis, but weak bands at 35, 45 and 50kDa appear from the regenerating connective tissues. Strongly immunolabeled bands for FGFR1 at 32, 60, and 80kDa and less intense for FGFR2 only appear in the regenerating tail...
November 2017: Annals of Anatomy, Anatomischer Anzeiger: Official Organ of the Anatomische Gesellschaft
https://www.readbyqxmd.com/read/28889021/maxillary-sinuses-and-midface-in-patients-with-cleidocranial-dysostosis
#20
Tomasz Kulczyk, Agnieszka Przystańska, Artur Rewekant, Renata Turska-Malińska, Agata Czajka-Jakubowska
The cleidocranial dysplasia is general skeletal disorder with an autosomal dominant inheritance. It is manifested by many craniofacial abnormalities, of which the maxillary hypoplasia is the most evident. The aim of the study was to use CBCT to evaluate the volume of the maxillary sinuses and the dimensions of maxillae in patients with CCD and compare them with healthy individuals. Files of four children with cleidocranial dysplasia were investigated. Volume of every maxillary sinus as well as two dimensional measurements of distances between particular points of interest located on surface of maxilla were calculated from reconstructed CBCT examination...
September 6, 2017: Annals of Anatomy, Anatomischer Anzeiger: Official Organ of the Anatomische Gesellschaft
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