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Journal of Immunoassay & Immunochemistry

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https://www.readbyqxmd.com/read/29309212/development-of-bead-based-multiplexed-immunoassay-for-evaluation-of-midkine-syndecan-1-and-angptl4-in-patient-serum
#1
Imad Tarhoni, Cristina L Fhied, Mark Pool, Michael J Liptay, Philip Bonomi, Christopher W Seder, Jeffrey A Borgia
BACKGROUND: Angiogenesis is associated with tumor progression in a range of malignancies. Herein, we develop custom immunobead assays for several mechanistically-important targets and evaluated these against sera from cohorts of non-small cell lung cancer (NSCLC) patients. METHODS: Antigen "capture" antibodies for midkine, syndecan-1, and ANGPTL4 were independently conjugated to MagPlex® Microspheres using standard carbodiimide/NHS-based chemistry. These reagents served as the basis for quantitative sandwich assay assembly using biotinylated detection antibodies and R-phycoerythrin-conjugated streptavidin reporter system...
January 8, 2018: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/29308976/about-molecular-profile-of-lung-cancer-in-tunisian-patients
#2
Faouzi Mezni, Mona Mlika, Hamouda Boussen, Habib Ghedira, Thouraya Fenniche, Talmoudi Faten, Marie-Anne Loriot
BACKGROUND: Molecular profile of lung cancer is well known in developed countries. These countries reached the era of liquid biopsies, immunotherapy and urine circulating tumor DNA. The descripancies between developed countries and developping ones are becoming deeper. Because of a lack of data in Tunisia, we tried to analyze the molecular profile of non small cell carcinomas and to assess the morphologic subtype of adenocarcinomas according to their mutational profile. METHODS: We performed molecular analyses in Tunisia and in France of 84 patients who were able to afford the cost of the diagnostic techniques carcinomas diagnosed between 2012 and 2015...
January 8, 2018: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/29308974/immunological-responses-against-vancomycin-resistant-enterococcus-faecium-and-enterococcus-faecalis-by-mice
#3
Yun Sang Cho, Mun Han Lee, Jong Man Kim, Hee Soo Lee, Han Sang Yoo, Yong Ho Park, Pan Dong Ryu
Methicillin-resistant Staphylococcus aureus (MRSA) infections in humans can currently only be treated with vancomycin. Consequently, vancomycin-resistant Enterococcus spp. pose a serious public health hazard because MRSA can acquire their vancomycin resistance. While the microbiological and genetic characteristics of vancomycin-resistant enterococci (VRE) have been extensively studied, serological diagnostic tools for these organisms are lacking. The VanA and VanB classes of VRE show marked resistance. Here, we identified the VanA and VanB proteins that are immunogenic in mice...
January 8, 2018: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/29308973/current-and-emerging-egfr-therapies-for-glioblastoma
#4
Stefan Alexandru Artene, Cristian Tuţă, Alexandra Dragoi, Oana Alexandru, Purcaru Stefana Oana, Daniela Elise Tache, Maria Mihaela Dănciulescu, Mihai Virgil Boldeanu, Cristian Adrian Siloşi, Anica Dricu
Glioblastomas (GBM) are the most lethal and hard to treat malignancies in clinical practice. The standard of care for treating GBM involving surgery and adjuvant radiotherapy and concomitant temozolomide (TMZ) has remained virtually unchanged in the past decade. Molecular targeted therapies against cancer-specific structures have reported mediocre results in the treatment of GBM, due to multiple factors such as the presence of the blood brain barrier (BBB) or a vast array of molecular alterations which greatly hinder the action of the most therapeutic agents...
January 8, 2018: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/29244613/kinetics-study-on-recombinant-alkaline-phosphatase-and-correlation-with-the-fluorescent-signal-generated
#5
Vanessa Susini, Veronica Lucia Rossi, Antonio Sanesi, Laurent Drazek
Alkaline phosphatase (AP) (EC 3.1.3.1) is one of the most used enzymes in immunoassays. In VIDAS® assays (bioMérieux, Marcy l'Etoile, France) AP catalyses the hydrolysis of 4-Methylumbelliferyl phosphate (4-MUP) in 4-Methylumbelliferone (4-MU) producing a fluorescent signal. This work introduces an original method of characterization of the kinetics parameter Km, Vmax and Kcat of AP embedded in VIDAS® assays. Assessment of such constants allows us to predict the fluorescent signal generated for given amounts of enzyme and its associated substrate; in the particular case of VIDAS®, it has been estimated that 0...
December 15, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/29144206/c-jun-expression-in-lichen-planus-psoriasis-and-cutaneous-squamous-cell-carcinoma-an-immunohistochemical-study
#6
Asmaa Gaber Abdou, Alaa Hassan Marae, Mohammed Shoeib, Ghada Dawood, Enas Abouelfath
The AP-1 transcription factor complex is a key player in regulating inflammatory processes, cell proliferation, differentiation and cell transformation. The aim of the present study is to investigate C-Jun (one of AP-1complex) expression and its proliferative role in skin samples of lichen planus, psoriasis as common inflammatory skin diseases and squamous cell carcinoma using immunohistochemical method. The present study was carried out on skin biopsies of 15 psoriatic patients, 15 lichen planus patients, 15 SCC and 15 normal skin biopsies...
November 16, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/29144198/development-of-novel-fluorescent-particles-applicable-for-phagocytosis-assays-with-human-macrophages
#7
Cecilia Sóñora, Paula Arbildi, Iris Miraballes-Martínez, Ana Hernández
Phagocytosis is a fundamental process for removal of pathogens and for clearance of apoptotic cells. The objective of this work was the preparation of fluorescent microspheres by a simple method and the evaluation of its applicability in phagocytosis assays by using different human derived cells, differentiated THP-1 cell line and blood monocytes, with flow cytometry measurements for functionality assays. Our results show that microparticles are efficiently internalized in a non-opsonized form and in dose dependent manner by both cellular types...
November 16, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/29144197/evaluation-of-umelisa-%C3%A2-t4-neonatal-and-umelisa-%C3%A2-t4-using-polystyrene-plates-coated-with-anti-thyroxine-t4-monoclonal-antibodies
#8
Elisa M Castells, Ernesto C González, Pedro L Pérez, Lesley Del Río, Yileidis Tejeda, Yenitse Perea, Odalys Martín, Maryeris Espinosa, Jose A Rivero, Amarilys Frómeta
Congenital hypothyroidism is one of the most common preventable causes of mental retardation. The Center of Immunoassay has developed the UMELISA(®) T4 NEONATAL and UMELISA(®) T4 to determine neonatal T4 levels in dried blood and serum samples. Both reagent kits use the same polystyrene plates coated with anti- thyroxine (T4) polyclonal antibodies as solid phase. This work shows the re-standardization of the UMELISA(®) T4 NEONATAL and UMELISA(®) T4 using plates coated with anti-T4 monoclonal antibodies (T4Mabs)...
November 16, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/29144195/extraction-purification-of-prostate-specific-antigen-psa-and-establishment-of-radioimmunoassay-system-as-a-diagnostic-tool-for-prostate-disorders
#9
Ahmed Sami Abu-Bakr El-Bayoumy, Akaber Tarek Hessien Keshta, Khaled Mohamed Sallam, Nahed Hassan Ebeid, Hatem Mohamed Elsheikh, Bsheer El-Sayed Bayoumy
This study aimed to provide an easy and effective method for extraction and purification of prostate specific antigen (PSA) from human seminal fluid with high quantity (14 mg) and high purity (98%). The obtained PSA was injected into rabbits for production of anti-PSA polyclonal antibody (titer 1/1000), labeled with radioactive iodine-125 for preparation of radioactive PSA tracer (purity 98 ± 1.8% and specific activity 64 ± 1.9 µCi/µg), and used in preparation of PSA standards. All prepared components can be used in PSA immunoassays specially radioimmunoassay (RIA) kit preparation as a diagnostic tool for prostatic diseases...
November 16, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/29144192/seeding-of-the-mucosal-leukocytes-in-the-halt-and-trachea-of-white-leghorn-chickens
#10
M Krunkosky, M García, L G Beltran Garza, E Karpuzoglu-Belgin, J Levin, R J Williams, R M Gogal
Characterizing the immune cells of the head associated lymphoid tissues (HALT) and trachea during maturation in young birds is critical to understanding the immunological responses to avian respiratory diseases and vaccines. Selected mucosal leukocytes of the conjunctiva-associated lymphoid tissue (CALT), Harderian gland (HG), nasal associated lymphoid tissue (NALT) and trachea from 4, 6, 8 and 10-week-old chickens were enumerated and phenotyped. HG, NALT, and trachea cellularity increased as the birds aged with cell viability varying by tissue...
November 16, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/29035165/development-of-highly-sensitive-cell-based-akt-kinase-elisa-for-monitoring-pi3k-beta-activity-and-compound-efficacy
#11
Mahesh Yanamandra, Labanyamoy Kole, Archana Giri, Sayan Mitra
Phosphatidylinositol-3 kinase (PI3K) pathway regulates multiple cellular functions involving cell survival, growth, motility proliferation, apoptosis, and adhesion. These are deregulated in various diseases such as cancer, atherosclerosis, and inflammation. PI3Ks phosphorylate phosphatidylinositol 4,5-biphosphate (PIP2) yielding phosphatidylinositol 3, 4, 5 triphosphate (PIP3) which in turn activate AKT kinase (serine/threonine kinase), the central enzyme in regulation of metabolic functions. Due to their implications in disease pathophysiology, PI3K/AKT inhibitors became attractive targets for pharmaceutical industries...
October 16, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/29035157/evaluation-of-serum-levels-of-c3-and-c4-complement-factors-in-patients-with-beta-thalassemia-major-in-khuzestan-province-southwest-iran
#12
Mehri Ghafourian, Mehrnosh Esmaeili, Alireza Sadeghi, Ali Malekei Naseri, Nader Dashti-Gerdabi
BACKGROUND AND OBJECTIVES: Thalassemia syndrome is the most common genetic disorder in the world and infection is the second cause of death in these patients. Measurement of serum C3 and C4 complement factors in serum was done in 60 patients with beta-thalassemia major in comparison with 30 healthy subjects as control group. MATERIALS AND METHODS: The serum level of C3 and C4 complement factors in 60 patients with beta-thalassemia major who were randomly selected from among the patients referred to Shafa Hospital of Ahvaz was evaluated and compared with 30 samples from healthy individuals with no history of recent infectious or autoimmune diseases...
October 16, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/29035147/isolation-production-and-characterization-of-a-new-single-chain-anti-idiotypic-antibody-against-benzo-a-pyrene
#13
K V Arnst, A E Studennikov, V A Ustinov, A N Glushkov
Polycyclic aromatic hydrocarbons are chemical carcinogens which could induce the development of human cancers. Anti-idiotypic antibodies against benzo[a]pyrene are perspective for human cancer immunoprophylaxis and tumor immunodiagnostic. The purpose of this study was isolating of anti-idiotypic antibodies against benzo[a]pyrene from human lymphocytes naïve phage library. The anti-idiotypic antibody, named B5, was selected. Analysis of the nucleotide and amino acid sequences B5 showed no similarity to known protein databases antibodies...
October 16, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/29035130/patterns-of-serologic-markers-of-hepatitis-b-virus-infection-and-the-risk-of-transmission-among-pregnant-women-in-southwestern-nigeria
#14
I M Ifeorah, A S Bakarey, M O Adewumi, T O C Faleye, A Akere, C E Omoruyi, A O Ogunwale, C C Uttah, M A Oketade, J A Adeniji
Hepatitis B virus (HBV) infection is a major health concern in developing countries that has a high morbidity and mortality rate. Vertical transmission of HBV from mother to child has been identified as a major factor leading to chronicity with attendant liver conditions, especially in poor socioeconomic settings. This study aims to evaluate the prevalence of serological HBV markers among pregnant women in Ibadan southwestern Nigeria and to determine the implications for perinatal HBV transmission. This study revealed the presence of varied HBV serological patterns of infection or immunity among pregnant women in Ibadan, Nigeria, and thus the risk of mother to child transmission...
October 16, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/28854102/seroprevalence-of-hepatitis-b-and-delta-viruses-among-hiv-infected-population-attending-antiretroviral-clinic-in-selected-health-facilities-in-abuja-nigeria
#15
I M Ifeorah, A S Bakarey, J A Adeniji, F N Onyemelukwe
INTRODUCTION: Tri- infection with human immunodeficiency virus (HIV), hepatitis B virus (HBV), and hepatitis D virus (HDV) is rare. There is limited data on the seroprevalence of HIV/HBV/HDV tri-infection especially in Nigeria. The aim of this study was to determine the seroprevalences of HBsAg and HDV among HIV infected individuals attending Anti-retroviral (ARV) clinics in Abuja, Nigeria. METHODS: In this cohort study, blood samples were collected from 1102 (Male = 450; Female = 652) age ranged <20 to ≥51 years (Mean age = 34...
August 30, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/28853996/effect-of-probiotic-strain-lactobacillus-acidophilus-lbkv-3-on-fecal-residual-lactase-activity-in-undernourished-children-below-10-year
#16
Sunil Tulshiram Hajare, Genene Bekele
Clinically proven Lactobacillus acidophilus strain LBKV-3 intended as probiotc for human was used to test its effect on fecal residual lactase activity in undernourished children below 10 years. The children were selected from malnutrition declared area of Maharashtra (India). One of the major causes of malnutrition is lactose intolerance which leads to diarrhea. The basic consideration in selecting the probiotic strain of Lactobacillus acidophilus-LBKV3 in this investigation was the fact that the organism is isolated from human vaginal surface swab and it was found extensively studied for probiotic characteristic...
August 30, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/28699851/dynamics-of-cd4-cell-count-among-hiv-infected-individuals-in-lagos-nigeria
#17
Babatunde O Motayo, Babatunde A Olusola, Adedayo O Faneye, Solomon Aturaka, Olukunle Oluwasemowo, Joseph I Ogiogwa
Human immunodeficiency virus (HIV) infection leads to progressive loss of CD4 T cells. Antiretroviral therapy has been able to inhibit this process, resulting in significant level of immune recovery and function. Our aim is to investigate the dynamics of CD4 recovery among HIV patients in Lagos, Nigeria. A total of 213 HIV-positive individuals were enrolled between October 2007 and May 2008, and followed up for 9 months based on CD4 count. CD4 analysis was done by flow cytometry at enrollment and after every 3 months...
July 12, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/28699839/validated-low-volume-immunoassay-for-the-reliable-determination-of-direct-renin-especially-valuable-for-pediatric-investigations
#18
J Schaefer, B B Burckhardt, J Tins, A Bartel, S Laeer
INTRODUCTION: The pharmacotherapy of pediatric patients suffering from heart failure is extrapolated from adults due to missing data in children. OBJECTIVES: Development and validation of a low-volume immunoassay for the reliable determination of renin. EXPERIMENTAL: The immunoassay was validated according to international guidelines. RESULTS: The assay allows the reliable determination of renin in 40 μL plasma within a calibration range of 4-128 pg/mL...
July 12, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/28699830/cag-pathogenicity-island-dependent-up-regulation-of-matrix-metalloproteinase-7-in-infected-patients-with-helicobacter-pylori
#19
Marzieh Sadeghiani, Nader Bagheri, Heshmat Shahi, Somayeh Reiisi, Ghorbanali Rahimian, Reza Rashidi, Majid Mahsa, Mohammedhadi Shafigh, Elaheh Salimi, Mahmoud Rafieian-Kopaei, Morteza Hashemzadeh-Chaleshtori, Hedayatollah Shirzad
Helicobacter pylori (H. pylori) infection has been involved in the pathogenesis of most important gastroduodenal diseases. Matrix metalloproteinases (MMPs) are a family of secreted, zinc-dependent enzymes collectively capable of degradation of extracellular matrix (ECM) and key player in various inflammatory diseases. Therefore, we examined MMP-7 mRNA levels in the gastric mucosa of patients with H. pylori infection and evaluated the effects of virulence factors, such as vacA (vacuolating cytotoxin A) and cagA (cytotoxin-associated gene) in H...
July 12, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/28692338/evaluation-of-a-novel-point-of-care-test-for-the-rapid-semi-quantitative-detection-of-allergen-specific-immunoglobulin-e
#20
René Rübenhagen, Marc Dangers
BACKGROUND: Allergen-specific IgE levels in human sera support the diagnosis of allergies. However, commonly used laboratory tests are time consuming. The new point-of-care test FastCheckPOC 20 (FCP20) allows the simultaneous and semi-quantitative evaluation of IgE antibodies for 20 aeroallergens and food allergens. METHODS: Two hundred patients with allergic symptoms were included in a multicenter performance evaluation using both whole blood and plasma or serum samples...
July 10, 2017: Journal of Immunoassay & Immunochemistry
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