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Journal of Immunoassay & Immunochemistry

Awoyelu E Hilda, Oloke Julius Kola, Oladipo E Kolawole
Hepatitis C virus is one of the emerging infectious diseases that can be transmitted through blood-to-blood contact. This study was carried out to determine the prevalence of anti-HCV antibodies among potential blood donors and pregnant women attending Bowen University Teaching Hospital (BUTH), Ogbomoso, Oyo State. Hospital based study was conducted from December 2014 to September 2015. The study group (N = 279) included potential blood donors and pregnant women. Data on socio-demographic characteristics and potential risk factors were collected using a structured questionnaire...
September 27, 2016: Journal of Immunoassay & Immunochemistry
Elisa María Castells Martínez, Ruben Del Valle, Ernesto Carlos González, Antonio Melchor, Pedro Lucio Pérez, Idania González, Adriana Carr, Kalet León
Human epidermal growth factor is a small peptide consisting of 53 amino acid residues, which stimulates cell proliferation and is associated with several human carcinomas. A simple sandwich-type ultramicroELISA assay (UMELISA), based on the advantages of high affinity reaction between streptavidin and biotin has been developed for the measurement of EGF in human serum samples. Strips coated with a high affinity monoclonal antibody directed against EGF are used as solid phase, to ensure the specificity of the assay...
September 16, 2016: Journal of Immunoassay & Immunochemistry
Jan E Binnie, Delwyn G Cooke, Leonard F Blackwell
An enzyme-linked immunosorbent assay (ELISA) for measurement of pregnanediol-3α-glucuronide (PdG) excretion rates in urine samples diluted to 150 mL/h before analysis is described. The sensitivity of the 9 optimized standard curves was 0.093 ± 0.070 μmol PdG/24 hr, with the multiple combined standard curves having a mean mid-point (EC50) of 6.88 μmol PdG/24 hr. The PdG threshold excretion rate of 7.0 μmol/24 hr, which is used as a marker for the end of fertility, was situated in the most accurate region of the standard curve...
September 16, 2016: Journal of Immunoassay & Immunochemistry
Kheir Eddine Kerboua, Fatma Haiba, Djamila Batouche
Treatment of atypical hemolytic uremic syndrome (aHUS) by the complement C5 inhibitor eculizumab (Soliris®) is highly effective but unfortunately, associated with an economic pressure on the health care systems even in high incomes countries. Despite spacing infusions has been proposed as the unique solution to minimize this economic impact, no reliable laboratory assays are available to tailor such therapy optimization. We aimed to propose and evaluate a complement composite marker for eculizumab efficacy monitoring in aHUS...
September 12, 2016: Journal of Immunoassay & Immunochemistry
David Zeman, Pavlína Kušnierová, Jana Bojková, František Všianský, Olga Zapletalová
IgG kappa and IgG lambda concentrations were quantified in 96 paired CSF and sera using Hevylite™ antibodies in an in-house developed sandwich ELISA method. In 56 of these samples, the results were compared with a qualitative isoelectric focusing/affinity-mediated immunoblotting assay for oligoclonal IgG kappa and IgG lambda. Normal IgG kappa/lambda ratio in the CSF was the same as in serum. In 19/33 patients with intrathecal oligoclonal IgG synthesis, skewed IgG kappa/lambda ratio was observed (increased in 16 and decreased in 3 cases)...
September 9, 2016: Journal of Immunoassay & Immunochemistry
Tsuyoshi Torii, Kentaku Kanemitsu, Akeo Hagiwara
Serum sialic acid (SA) levels are important for diagnosis, follow-up, and mechanistic analysis of malignant diseases. However, little is known about the levels of SA bound to serum IgM. Here, we isolated IgM from sera of healthy individuals and patients with cancer using DEAE chromatography and 8% polyethylene glycol precipitation. In this fraction, which contained partially purified IgM (recovery; 52%; purity: 25%), SA was quantified with fluorescence detection-HPLC (detection limit: 0.08 μM). SA levels in the IgM-enriched fraction was significantly higher in cancer patients (104 ± 27 µM) than in healthy individuals (81 ± 11 µM; p = 0...
September 6, 2016: Journal of Immunoassay & Immunochemistry
Tuba Ozgocer, Sedat Yildiz, Cihat Uçar
Non-invasive measurement of cortisol in saliva is of prime importance as it represents a bioavailable neuroendocrine marker for stress. Therefore, in this study, we developed an enzyme immune assay that was suitable for salivary cortisol measurements. For that purpose, rabbit polyclonal antibody was raised against cortisol-3-CMO:BSA conjugate. The test was based on competition of liquid phase cortisol with conjugated cortisol on the solid phase. Primary antibody was used to bind available sites on the conjugate, which was proportional to numbers of cortisol in liquid phase...
September 6, 2016: Journal of Immunoassay & Immunochemistry
Rafid Mustafa, Elliott Blumenthal
Experiments were conducted to examine the effects of turmeric on spleen cell proliferation. Cell suspensions of spleen cells from young and aged mice were treated with or without conconavalin A (Con-A) as a proliferation stimulant, and with and without turmeric (20 mg/ml) in different concentrations. Spleen cells from young mice that received turmeric showed significant increase in spleen cell proliferation (p<0.05), while spleen cells from aged mice that received turmeric showed no significant increase in T lymphocytes...
August 25, 2016: Journal of Immunoassay & Immunochemistry
Majid Asiabanha Rezaee, Mohammad Javad Rasaee, Javad Mohammadnejad
Human cardiac troponin I (cTni) is the gold marker for early diagnosis of myocardial infarction. In this regard, four immune-dominant epitopes of cTni were predicted and their 3D structures were determined. Thereafter, the competitive performance of the peptides was monitored with the developed polyclonal antibody-based indirect competitive ELISA; a half-maximal inhibitory concentration (IC50) of 0.49 (µg/mL) and detection limit of 0.037 (µg/mL) were achieved for recombinant cTni. The competitive ELISA determined sensitivity levels of 0...
August 11, 2016: Journal of Immunoassay & Immunochemistry
Opeyemi Mayowa Onilude, Sabri Mohd Yusoff, Benjamin Obukowho Emikpe, Polycarp Tanko, Salisi M Shahrom, Mohammed Effendy
Early and accurate diagnosis of Brucella melitensis is essential for the treatment and control of brucellosis both in animals and humans. The thrust for the development of a rapid diagnostic technique to overcome the limitations of conventional microbiological and serological tests brought about this investigation on the development and application of dot-ELISA for antigen and antibody detection in infected goats. Fifteen apparently healthy Boer aged 2-3 years which tested negative for brucellosis using PCR and ELISA, were grouped into A (10 goats infected intraocularly with 10(7) CFU of B...
August 5, 2016: Journal of Immunoassay & Immunochemistry
Mona Mlika, Nourane Kria, Emna Braham, Chokri Chebbi, Faouzi El Mezni
BACKGROUND: Multidisciplinary concertation is mandatory in order to assess interstitial pneumonias. The study of the bronchoalveolar lavage helps evoking a diagnosis according to the lavage profile. In lymphocytic alveolitis, immunocytochemistry or in flux cytometry are necessary in order to identify the different clusters of lymphocytes implicated. AIM: Our objective was to evaluate the profile of 31 lymphocytic alveolitis using 2 different techniques which are the immunocytochemistry and the in flow cytometry in order to evaluate the efficacy of each technique and to compare the different results to the final diagnoses...
July 29, 2016: Journal of Immunoassay & Immunochemistry
Jaime Pérez-Griera, Juan Carlos Andreu-Ballester, Alejandro Hueso Zarandieta, José García de la Asunción, Silvia Masquefa Bondia
Secretory immunoglobulin A (SIgA) in serum is possibly the best index of SIgA presence in mucosal secretions in digestive tract and the mirror of its immunologic barrier against many pathogenic aggressions. The measurement of salivary SigA alone may be affected by total salivary secretion and its final concentration in the gland lumen is probably not useful as a appropriate index of mucosal secretions in the digestive tract. The usefulness of the determination of SigA against various epitopes in serum from patients with various autoimmune disease has been demonstrated...
July 25, 2016: Journal of Immunoassay & Immunochemistry
Prashant Giribhattanavar, Kavitha Kumar, Siddarth Raajasekar, Nagarathna Chandrashekar, Shripad Patil
Mycobacterium tuberculosis (H37Ra) culture filtrate proteins (CFP) are explored as a diagnostic marker for tuberculous meningitis (TBM). Cerebrospinal fluid (CSF) samples from patients were categorized as confirmed (n=47), suspected (n=20) and non-TBM (n=25) cases. Immune response by western-blot revealed TBM CSF samples are having heterogeneous response to CFP. CFP ELISA was 92% sensitive and 38.30% specific. ODs of confirmed TBM and non-TBM cases were significantly different (P<0.0001) and also the suspected TBM and non-TBM cases (P=0...
July 25, 2016: Journal of Immunoassay & Immunochemistry
Mamta Pandey, B V Sunil Kumar, Satparkash Singh, Ramneek Verma
Matrix metalloproteinase-3 is invariably upregulated in cancerous condition. So we aimed to determine serum level of MMP-3 in canine mammary tumors. The gene was expressed in E. coli system as ~43kDa recombinant protein, which was refolded, purified and confirmed. Hyperimmune serum was raised against the expressed protein in rabbits and mice to standardize sandwich ELISA. ROC analysis revealed largest area under the curve of 0.998 with sensitivity (100%) and specificity (95%) for a cut-off value of 0.363 with respect to histopathological staining...
July 12, 2016: Journal of Immunoassay & Immunochemistry
Florentina Serban, Oana Daianu, Ligia Gabriela Tataranu, Stefan-Alexandru Artene, Ghazaleh Emami, Ada Maria Georgescu, Oana Alexandru, Stefana Oana Purcaru, Daniela Elise Tache, Maria Mihaela Danciulescu, Veronica Sfredel, Anica Dricu
The failure of therapies targeting tumor angiogenesis may be caused by anti-angiogenic resistance mechanisms induced by VEGF and non-VEGF pathways alterations. Anti-angiogenic therapy failure is also attributed to immune system, acting by tumor-associated macrophages that release pro-angiogenic factors and a consequent increase of blood vessels. Recently, in a study by Rheal A. et, a new angiogenic receptor, epidermal growth factor, latrophilin, and 7 trans-membrane domain-containing protein 1 on chromosome 1(ELTD1) has been identified as a promising glioma biomarker...
July 5, 2016: Journal of Immunoassay & Immunochemistry
Saima Naz, Farkhanda Ghafoor, Imran Akhtar Iqbal, S M Saqlan Naqvi
C-reactive protein (CRP) is intricately sensitive marker of inflammation, infection and tissue damage. Role in the prognosis of heart diseases has been recently discovered. This study aimed to develop a cost effective and high sensitivity CRP immunoassay for use in cardiac risk assessment. Assay was optimized for coating, blocking of capturing antibody, dilution & reaction time of the conjugate and sample volume. For normal reference range, CRP was determined in serum samples from apparently healthy volunteers...
July 1, 2016: Journal of Immunoassay & Immunochemistry
S A Nassar, T O Oyekale, A S Oluremi
Cryptosporidiosis is an important major cause of enteric parasitic infection among infants and children in developing countries with morbidity and mortality especially among immune- deficient individuals. Poor performance of traditional diagnostic procedures in many laboratories can resulted in misdiagnosis and mistreatment, thereby probably promotes drug resistance. This study was carried out to determine the prevalence of enteric Cryptosporidiosis in children attending primary health centers in Egbedore Local government area of Osun state, Nigeria between July to December, 2014...
May 9, 2016: Journal of Immunoassay & Immunochemistry
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No abstract text is available yet for this article.
2016: Journal of Immunoassay & Immunochemistry
Xin Li, Stephen L Kaattari, Mary Ann Vogelbein, Michael A Unger
The development of monoclonal antibodies (mAb) with affinity to small molecules can be a time-consuming process. To evaluate shortening the time for mAb production, we examined mouse antisera at different time points post-immunization to measure titer and to evaluate the affinity to the immunogen PBA (pyrene butyric acid). Fusions were also conducted temporally to evaluate antibody production success at various time periods. We produced anti-PBA antibodies 7 weeks post-immunization and selected for anti-PAH reactivity during the hybridoma screening process...
2016: Journal of Immunoassay & Immunochemistry
Shamsi Naderi, Zahra Hejazi, Mansour Shajarian, Fereshteh Alsahebfosoul, Masoud Etemadifar, Nahid Sedaghat
Interleukin-27 (IL-27) is a member of IL-6/IL-12 cytokine family which possesses pro- and anti-inflammatory properties and participates in the pathogenesis of various autoimmune diseases. In our case-control study, plasma was collected from healthy subjects as control group (n = 40) and patients with relapsing-remitting multiple sclerosis (RRMS) (n = 40), including new identified cases without treatment (n = 12) and those treated with Interferon beta (IFN-β) (n = 28). The plasma level of IL-27 was assessed by ELISA method...
2016: Journal of Immunoassay & Immunochemistry
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