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Journal of Immunoassay & Immunochemistry

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https://www.readbyqxmd.com/read/28027008/development-of-an-immunoassay-using-an-anti-wogonin-glucuronide-monoclonal-antibody
#1
Madan Kumar Paudel, Seiichi Sakamoto, Le Van Huy, Hiroyuki Tanaka, Miyamoto Tomofumi, Takano Akihito, Satoshi Morimoto
Wogonin 7-O-β-D-glucuronide (Wgn) is a bioactive flavone present in the dried root of Scutellaria baicalensis Georgi. To generate a monoclonal antibody (MAb) against Wgn, BALB/c mice injected with Wgn-bovine serum albumin yielded splenocytes that we fused with SP2/0 myeloma cells using the polyethylene glycol method. We obtained a hybridoma designated 315A that produced a MAb reactive to Wgn. The anti-Wgn MAb 315A was applied to an indirect competitive enzyme-linked immunosorbent assay (icELISA) to quantify Wgn...
December 27, 2016: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/28026995/scale-up-production-and-characterization-of-anti-human-cardiac-troponin-i-monoclonal-antibody-in-ascitic-fluid-of-balb-c-mice
#2
Asiabanha Rezaee Majid, Rasaee Mohammad Javad, Paknejad Malihe, Mohammadnejad Javad
The Human Cardiac Troponin I (hcTnI) is a 210 amino acid protein, 23 kDa in molecular weight. This biomarker is commonly used to diagnose myocardial infarction, micro injury, and acute coronary syndrome (ACS) in patients referring to emergency departments. The American Heart Association (AHA) and European Society of Cardiology (ESC) proposed troponin I as the gold biomarker for early detection of heart attack, especially in myocardial infarction (MI). Therefore, developing monoclonal antibodies against this biomarker could help in for early detection of heart attack...
December 27, 2016: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/28010157/evaluation-of-immune-response-in-challenged-chickens-vaccinated-with-newcastle-disease-vaccine-using-gums-from-cedrela-odorata-and-khaya-senegalensis-as-delivery-agents
#3
V O Oyebanji, B O Emikpe, Omolade A Oladele, M O Odeniyi, A Salami, O I Osowole, O B Kasali, O A Akinboade
Immune response of challenged chickens following previous vaccinations with Newcastle disease vaccine using gums from Cedrela odorata and Khaya senegalensis as delivery agent were evaluated. Two hundred and fifty-two (252) one-day old chickens were divided into vaccine-gum oral (GVOR), vaccine-gum ocular (GVOC), vaccine oral (VOR), vaccine ocular (VOC), gum oral (GOR), gum ocular (GOC), No-gum-no-vaccine/challenged (NGNV/C) & No-gum-no-vaccine/unchallenged (NGNV/U) groups. They were vaccinated at days 21 & 42 and challenged at day 84...
December 23, 2016: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/27982728/a-comparison-of-nanoparticle-antibody-conjugation-strategies-in-sandwich-immunoassays
#4
Justina O Tam, Helena de Puig, Chun-Wan Yen, Irene Bosch, Jose Gómez-Márquez, Charles Clavet, Kimberly Hamad-Schifferli, Lee Gehrke
Point-of-care (POC) diagnostics such as lateral flow and dipstick immunoassays use gold nanoparticle (NP)-antibody conjugates for visual readout. We investigated the effects of NP conjugation, surface chemistries, and antibody immobilization methods on dipstick performance. We compared orientational, covalent conjugation, electrostatic adsorption, and a commercial conjugation kit for dipstick assays to detect dengue virus NS1 protein. Assay performance depended significantly on their conjugate properties. We also tested arrangements of multiple test lines within strips...
December 16, 2016: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/27929756/spontaneous-mouse-mammary-tumor-cell-lysates-induce-igg-production-in-spleen-mononuclear-cells-of-healthy-and-tumor-bearing-mice
#5
Ahad Khalilnezhad, Elham Mahmoudian, Nariman Mosaffa, Jaleh Mohsenifar, Davar Amani
BACKGROUND: We hypothesized that Tumor cell lysate (TCL) prepared from spontaneous mouse mammary tumor (SMMT) may elicit IgG production by spleen mononuclear cells (SMCs) in ex vivo. METHODS: The SMCs from healthy mice (HM, n = 6) and four-week SMMT-bearing mice (TBM, n = 6) was cultured in presence of TCL and mitogen for 42 hr at 37°C, separately. Serum and SMCs culture supernatant levels of IFNγ, IL-4, and total IgG were measured using special ELISA kits. RESULTS: Serum IgG level of TBM was significantly higher than that of HM group (P = 0...
December 8, 2016: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/27892811/simultaneous-determination-of-glycyrrhizin-and-liquiritin-in-licorice-roots-and-kampo-medicines-by-combination-enzyme-linked-immunosorbent-assay-using-anti-glycyrrhizin-and-anti-liquiritin-monoclonal-antibodies
#6
Shunsuke Fujii, Osamu Morinaga, Takuhiro Uto, Shuichi Nomura, Yukihiro Shoyama
Immunoassay systems using monoclonal antibodies (mAbs) are one of the most useful techniques in the analytical, biochemical, and clinical fields. In this study, a combination enzyme-linked immunosorbent assay (ELISA) using both anti-glycyrrhizin and anti-liquiritin mAbs (anti-GL/Liq mixture mAbs) was developed for quality control of licorice and its products. The combination ELISA demonstrated high sensitivity, reproducibility, and specificity for the total content of GL and Liq by a single assay. The developed ELISA was effective and useful as the first screening method in the selection of high-quality licorice from the Glycyrrhiza species and in confirming the quality of licorice-containing Kampo medicines...
November 28, 2016: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/28102778/highlights-of-new-immunoassay-based-technologies
#7
Cristiana Tanase, Radu Albulescu, Monica Neagu
No abstract text is available yet for this article.
2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/27797300/a-new-covalent-peroxidase-conjugation-method-using-bis-sulfosuccinimidyl-suberate-as-cross-linking-reagent-in-a-two-step-procedure
#8
Rivo Presentini
A new method has been developed to prepare horseradish peroxidase (HRP) conjugates using bis(sulfosuccinimidyl)suberate (BS3) as cross-linking reagent in a two-step procedure. The enzyme is reacted with BS3, introducing active ester molecules into the enzyme itself, and it is then used directly to label amino-containing compounds without further treatment. The proteins involved in the conjugation undergo minimal modifications. The reaction conditions are evaluated, as well as studies on the conservation of the biological activities of the conjugated proteins and the stability of the conjugates in time...
2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/27624975/selection-of-specific-inhibitor-peptides-in-enzyme-linked-immunosorbent-assay-elisa-of-cardiac-troponin-i-using-immuno-dominant-epitopes-as-competitor
#9
Majid Asiabanha Rezaee, Mohammad Javad Rasaee, Javad Mohammadnejad
Human cardiac troponin I (cTni) is the gold marker for early diagnosis of myocardial infarction. In this regard, four immune-dominant epitopes of cTni were predicted and their 3D structures were determined. Thereafter, the competitive performance of the peptides was monitored with the developed polyclonal antibody-based indirect competitive ELISA; a half-maximal inhibitory concentration (IC50) of 0.49 (µg/mL) and detection limit of 0.037 (µg/mL) were achieved for recombinant cTni. The competitive ELISA determined sensitivity levels of 0...
2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/27494045/development-and-application-of-dot-enzyme-linked-immunosorbent-dot-elisa-assay-for-detection-of-brucella-melitensis-and-evaluation-of-the-shedding-pattern-in-infected-goats
#10
Opeyemi Mayowa Onilude, Sabri Mohd Yusoff, Benjamin Obukowho Emikpe, Polycarp Tanko, Salisi M Shahrom, Mohammed Effendy
Early and accurate diagnosis of Brucella melitensis is essential for the treatment and control of brucellosis both in animals and humans. The thrust for the development of a rapid diagnostic technique to overcome the limitations of conventional microbiological and serological tests brought about this investigation on the development and application of dot-ELISA for antigen and antibody detection in infected goats. Fifteen apparently healthy Boer aged 2-3 years which tested negative for brucellosis using PCR and ELISA, were grouped into A (10 goats infected intraocularly with 10(7) CFU of B...
2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/27471881/the-diagnostic-importance-of-the-bronchoalveolar-lavage-in-lymphocytic-alveolitis
#11
Mona Mlika, Nourane Kria, Emna Braham, Chokri Chebbi, Faouzi El Mezni
Multidisciplinary concertation is mandatory in order to assess interstitial pneumonias. The study of the bronchoalveolar lavage helps evoking a diagnosis according to the lavage profile. In lymphocytic alveolitis, immunocytochemistry, or in flux cytometry are necessary in order to identify the different clusters of lymphocytes implicated. Our objective was to evaluate the profile of 31 lymphocytic alveolitis using 2 different techniques which are the immunocytochemistry and the in flow cytometry in order to evaluate the efficacy of each technique and to compare the different results to the final diagnoses...
2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/27454420/a-quantitative-enzyme-linked-immunosorbent-assay-for-quantification-of-secretory-immunoglobulin-a-in-serum
#12
Jaime Pérez-Griera, Juan Carlos Andreu-Ballester, Alejandro Hueso Zarandieta, José García de la Asunción, Silvia Masquefa Bondia
Secretory immunoglobulin A (SIgA) in serum is possibly the best index of SIgA presence in mucosal secretions in digestive tract and the mirror of its immunologic barrier against many pathogenic aggressions. The measurement of salivary SigA alone may be affected by total salivary secretion and its final concentration in the gland lumen is probably not useful as an appropriate index of mucosal secretions in the digestive tract. The usefulness of the determination of SigA against various epitopes in serum from patients with various autoimmune disease has been demonstrated...
2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/27454259/significance-of-immune-response-to-mycobacterium-tuberculosis-culture-filtrate-protein-antigens-in-cerebrospinal-fluid-of-tuberculous-meningitis-patients-a-search-for-diagnostic-marker
#13
Prashant Giribhattanavar, Kavitha Kumar, Siddarth Raajasekar, Nagarathna Chandrashekar, Shripad A Patil
Mycobacterium tuberculosis (H37Ra) culture filtrate proteins (CFP) are explored as a diagnostic marker for tuberculous meningitis (TBM). Cerebrospinal fluid (CSF) samples from patients were categorized as confirmed (n = 47), suspected (n = 20), and non-TBM (n = 25) cases. Immune response by Western blot revealed TBM CSF samples are having heterogeneous response to CFP. CFP ELISA was 92% sensitive and 38.30% specific. ODs of confirmed TBM and non-TBM cases were significantly different (P < 0.0001) and also the suspected TBM and non-TBM cases (P = 0...
2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/27404490/development-of-recombinant-matrix-metalloproteinase-3-based-sandwich-elisa-for-sero-diagnosis-of-canine-mammary-carcinomas
#14
Mamta Pandey, B V Sunil Kumar, Satparkash Singh, Ramneek Verma
Matrix metalloproteinase-3 is invariably upregulated in cancerous condition. So we aimed to determine serum level of MMP-3 in canine mammary tumors. The gene was expressed in E. coli system as ~43kDa recombinant protein, which was refolded, purified, and confirmed. Hyperimmune serum was raised against the expressed protein in rabbits and mice to standardize sandwich ELISA. ROC analysis revealed largest area under the curve of 0.998 with sensitivity (100%) and specificity (95%) for a cut-off value of 0.363 with respect to histopathological staining...
2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/27379831/silencing-of-epidermal-growth-factor-latrophilin-and-seven-transmembrane-domain-containing-protein-1-eltd1-via-sirna-induced-cell-death-in-glioblastoma
#15
Florentina Serban, Oana Daianu, Ligia Gabriela Tataranu, Stefan-Alexandru Artene, Ghazaleh Emami, Ada Maria Georgescu, Oana Alexandru, Stefana Oana Purcaru, Daniela Elise Tache, Maria Mihaela Danciulescu, Veronica Sfredel, Anica Dricu
The failure of therapies targeting tumor angiogenesis may be caused by anti-angiogenic resistance mechanisms induced by VEGF and non-VEGF pathways alterations. Anti-angiogenic therapy failure is also attributed to immune system, acting by tumor-associated macrophages that release pro-angiogenic factors and a consequent increase of blood vessels. Recently, in a study by Rheal et al., a new angiogenic receptor, epidermal growth factor, latrophilin, and 7 trans-membrane domain-containing protein 1 on chromosome 1(ELTD1) has been identified as a promising glioma biomarker...
2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/27366878/development-of-a-high-sensitivity-c-reactive-protein-immunoassay-and-comparison-with-a-commercial-kit
#16
Saima Naz, Farkhanda Ghafoor, Imran Akhtar Iqbal, S M Saqlan Naqvi
C-reactive protein (CRP) is intricately sensitive marker of inflammation, infection, and tissue damage. Role in the prognosis of heart diseases has been recently discovered. This study aimed to develop a cost-effective and high-sensitivity CRP immunoassay for use in cardiac risk assessment. Assay was optimized for coating, blocking of capturing antibody, dilution, and reaction time of the conjugate and sample volume. For normal reference range, CRP was determined in serum samples from apparently healthy volunteers...
2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/27158941/prevalence-of-cryptosporidium-infection-and-related-risk-factors-in-children-in-awo-and-iragberi-nigeria
#17
S A Nassar, T O Oyekale, A S Oluremi
Cryptosporidiosis is an important major cause of enteric parasitic infection among infants and children in developing countries with morbidity and mortality especially among immune- deficient individuals. Poor performance of traditional diagnostic procedures in many laboratories can resulted in misdiagnosis and mistreatment, thereby probably promotes drug resistance. This study was carried out to determine the prevalence of enteric Cryptosporidiosis in children attending primary health centers in Egbedore local government area of Osun State, Nigeria between July-December 2014...
2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/27854157/measuring-fecal-testosterone-metabolites-in-spotted-hyenas-choosing-the-wrong-assay-may-lead-to-erroneous-results
#18
Susanne Pribbenow, Tulsidas G Shrivastav, Martin Dehnhard
Enzyme-immunoassays (EIA) that detect fecal testosterone metabolites (fTM) are powerful tools to monitor gonadal activity non-invasively. However, a challenge with testosterone EIAs might be their potential for cross-reactivities with structurally similar glucocorticoid metabolites. Therefore, we aimed to verify the capability of four different testosterone EIAs to monitor fTM without reflecting changes in adrenocortical activity in spotted hyenas by analyzing fecal samples following testosterone and ACTH challenge tests...
November 17, 2016: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/27854146/immunoassay-and-molecular-methods-to-investigate-dna-methylation-changes-in-peripheral-blood-mononuclear-cells-in-hiv-infected-patients-on-cart
#19
Adelina Rosca, Gabriela Anton, Luminita Ene, Iulia Iancu, Aura Temereanca, Cristian L Achim, Simona M Ruta
This study aimed to investigate the influence of antiretroviral therapy on methylation markers, in a group of HIV infected, heavily treated patients. Immune and molecular methods were used to investigate potential changes in methylation profile in DNA isolated from peripheral blood mononuclear cells collected from antiretroviral-experienced HIV infected patients and healthy controls. The percentage of 5-methylcytosine was inversely correlated with proviral DNA and active replication while DNMT1 (p = 0.01) and DNMT3A (p = 0...
November 17, 2016: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/27801618/a-multi-analyte-immunoassay-for-thyroid-related-analytes
#20
Bharti Jain, J Kumarasamy, C Gholve, Savita Kulkarni, M G R Rajan
We describe the development and validation of multianalyte immunoassays (MAIA) for three analytes, viz., thyroxine (T4), thyroid stimulating hormone (TSH), and thyroglobulin (Tg) essential for assessment of thyroid function but having widely varying molecular weights. Using polycarbonate (PC) track-etched membranes (TEM) as an immobilization support and (125)I as the tracer, both competitive assay for T4 and non-competitive assay for TSH and Tg were performed on the same TEM. MAIA was found to be highly sensitive and precise with clinically useful working range and correlated very well with individual analyte immunoassays...
November 1, 2016: Journal of Immunoassay & Immunochemistry
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