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Journal of Immunoassay & Immunochemistry

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https://www.readbyqxmd.com/read/29771632/immunohistochemical-expression-hif1%C3%AE-in-chronic-plaque-psoriasis-an-association-with-angiogenesis-and-proliferation
#1
Asmaa Gaber Abdou, Azza G A Farag, Moustafa Hammam, Doha Maher Taie, Reem Ahmed Abdelaziz
Psoriasis is characterized by excessive cell proliferation, angiogenesis, and regions of hypoxia. Hypoxia stimulates production of hypoxia inducible factors (HIFs) such as HIF1α. The aim of the present study is to investigate the possible role of HIF1α in pathogenesis of psoriasis and to correlate its expression with angiogenesis and proliferation in involved and uninvolved skin in patients with plaque psoriasis using CD34 and Ki-67. The current study was performed on 40 skin specimens of patients presented with chronic plaque psoriasis both involved and uninvolved together with 40 specimens from age- and sex-matched healthy volunteers as a control group...
May 17, 2018: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/29764292/evaluation-of-performance-testing-of-different-rapid-diagnostic-kits-in-comparison-with-eias-to-validate-detection-of-hepatitis-b-virus-among-high-risk-group-in-nigeria
#2
Abosede Yetunde Afolabi, Adeleye Solomon Bakarey, Moses Olubusuyi Adewumi
BACKGROUND: Hepatitis B virus (HBV) causes chronic liver-associated diseases and its early detection is of high public health importance. Its diagnosis is mainly based on immunological assays among which Enzyme-Linked Immunosorbent Assay (ELISA) and rapid tests are the most common and widespread methods. However, a major challenge is the discordance of results of any two laboratory assays which cannot be easily resolved. Therefore, this study was designed to evaluate the validity and reliability of commercially available five rapid test kits in comparison with two Enzyme Immunoassays (EIAs) in Nigeria using hepatitis B surface antigen as a reference marker...
May 15, 2018: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/29757709/how-to-assess-the-best-immunohistochemical-panel-in-the-diagnosis-of-malignant-pleural-mesothelioma-in-a-pathology-lab
#3
Mona Mlika, Oumeima Lamzibri, Saoussen Bacha, Soumeya Laabidi, Chokri Haddouchi, Faouzi El Mezni
BACKGROUND: malignant pleural mesothelioma (MPM) is a rare tumor with a challenging diagnosis. Even if, clinical data are mandatory to suspect the diagnosis, the positive diagnosis is based on microscopic features. Morphologic features are still the port of call of the diagnosis but their non specific character and the multiplicity of differential diagnoses made the immunohistochemical markers mandatory for the diagnosis. Many antibodies with a positive diagnostic value including claretinin, mesothelin, WT1 and antibodies with a negative diagnostic value including TTF1, EMA, CD15 are recommended by the scientific societies...
May 14, 2018: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/29757708/complete-blood-count-alterations-due-to-the-opioid-use-what-about-the-lymphocyte-related-ratios-especially-in-monocyte-to-lymphocyte-ratio-and-platelet-to-lymphocyte-ratio
#4
Mehmet Hamdi Orum, Mahmut Zabit Kara, Oguzhan Bekir Egilmez, Aysun Kalenderoglu
This study was designed to compare the complete blood count values of opioid users (N = 61) and healthy subjects (N = 61), particularly monocyte-to-lymphocyte ratio (MLR) and platelet-to-lymphocyte ratio (PLR). PLR, MLR, and percentage of monocyte (MONO%) were significantly lower in opioid use disorder (OUD) group (P = 0.012, P = 0.005, P = 0.000). The area under the ROC curve of MLR and PLR levels for OUD was 0.349 and 0.368. MONO% correlated with substance use duration. Measurements like lymphocyte-related ratios and MONO% in opioid use can be important in substance monitoring, detection, and differentiation of acute and chronic conditions...
May 14, 2018: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/29757703/hepatitis-b-virus-infection-among-asymptomatic-residents-of-low-income-community-in-ibadan-southwest-nigeria
#5
Adeleye Solomon Bakarey, Oluwaseun Deborah Olaniyan
INTRODUCTION: Hepatitis B Virus (HBV) infection is one of the most infectious diseases worldwide and a major public health concern. In spite of efforts at controlling the scourge globally, HBV continues to thrive in developing countries, such as Nigeria due to ignorance on its mode of transmission and its asymptomatic nature in the populace. Therefore, this community-based study was carried out in Yemetu community in Ibadan, Nigeria to determine the burden of HBV infection among asymptomatic residents of this community...
May 14, 2018: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/29741994/development-of-a-novel-explant-culture-method-for-the-isolation-of-mesenchymal-stem-cells-from-human-breast-tumor
#6
Koushan Sineh Sepehr, Alireza Razavi, Mohsen Saeidi, Majid Mossahebi-Mohammadi, Meghdad Abdollahpour-Alitappeh, Seyed Mahmoud Hashemi
BACKGROUND: Mesenchymal stem cells (MSCs) were isolated from various sources, including various types of tumors. However choosing an appropriate isolation method is an important step in obtaining cells with optimal quality and yield in companion with economical considerations. The purpose of this study was to isolate of more pure MSCs from human breast tumor tissue by a modified explant culture method. METHODS AND MATERIALS: The tumor tissues (n = 8) was cut into 1 to 3-mm cube-like pieces (explant)...
May 9, 2018: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/29733758/comparison-of-the-performance-of-enzyme-immunoassays-for-hepatitis-b-and-c-detection-in-dried-blood-spot
#7
Helena Medina Cruz, Juliana Custódio Miguel Cruz, Elisangela Ferreira Da Silva, Moyra Machado Portilho, Vanessa Alves Marques, Lia Laura Lewis-Ximenez, Elisabeth Lampe, Livia Melo Villar
Dried blood spots (DBSs) could be an alternative to serum for hepatitis B virus (HBV) and hepatitis C virus (HCV) diagnosis. This study aims to evaluate two enzyme immunoassays (EIAs) for HBsAg and anti-HCV detection using DBS. Serum was tested using commercial EIA. DBS was tested using optimized EIA developed for serum and commercial EIA developed for DBS (Imunoscreen). Concordances between DBS and serum samples for both markers and EIAs were higher than 97%. Both EIAs demonstrated good performance for HBsAg and anti-HCV detection using DBS, and these methods could be used unchangeably increasing the access for HBV and HCV diagnosis...
May 7, 2018: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/29424653/seroprevalence-of-hepatitis-b-surface-antigenaemia-and-viral-infectivity-among-liver-cancer-patients-accessing-care-at-a-tertiary-health-facility-in-southwest-nigeria
#8
Adeleye Solomon Bakarey, Olukemi Jabaru, Olusola Olayiwola
No abstract text is available yet for this article.
February 9, 2018: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/29424631/occurrence-of-infectious-laryngotracheitis-outbreaks-in-commercial-layer-hens-detected-by-elisa
#9
Zeki Aras, Orhan Yavuz, Gökçenur Sanioğlu Gölen
Infectious laryngotracheitis (ILT) is an acute respiratory disease of chickens and a cause of great economic loss in commercial layers. The aims of this study were to investigate the prevalence of ILT in the field outbreaks and to compare the characteristics of ILT-infected and free flocks of commercial layers. A total of 625 blood serum samples were collected from 25 different layer flocks. The presence of antibodies against infectious laryngotracheitis virus (ILTV) in each sample was determined by ELISA. Of the 625 serum samples, 266 (42...
February 9, 2018: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/29364086/the-inositol-1-2-cyclic-phosphate-moiety-of-the-cross-reacting-determinant-carbohydrate-chains-and-proteinaceous-components-are-all-responsible-for-the-cross-reactivity-of-trypanosome-variant-surface-glycoproteins
#10
José L Escalona, Graciela L Uzcanga, Liomary M Carrasquel, José Bubis
Salivarian trypanosomes evade the host immune system by continually swapping their protective variant surface glycoprotein (VSG) coat. Given that VSGs from various trypanosome stocks exhibited cross-reactivity (Camargo et al., Vet. Parasitol. 207, 17-33, 2015), we analyzed here which components are the antigenic determinants for this cross-reaction. Soluble forms of VSGs were purified from four Venezuelan animal trypanosome isolates: TeAp-N/D1, TeAp-ElFrio01, TeAp-Mantecal01, and TeGu-Terecay323. By using the VSG soluble form from TeAp-N/D1, we found that neither the inositol-1,2-cyclic phosphate moiety of the cross-reacting determinant nor the carbohydrate chains were exclusively responsible for its cross-reactivity...
January 24, 2018: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/29308974/immunological-responses-against-vancomycin-resistant-enterococcus-faecium-and-enterococcus-faecalis-by-mice
#11
Yun Sang Cho, Mun Han Lee, Jong Man Kim, Hee Soo Lee, Han Sang Yoo, Yong Ho Park, Pan Dong Ryu
Methicillin-resistant Staphylococcus aureus (MRSA) infections in humans can currently only be treated with vancomycin. Consequently, vancomycin-resistant Enterococcus spp. pose a serious public health hazard because MRSA can acquire their vancomycin resistance. While the microbiological and genetic characteristics of vancomycin-resistant enterococci (VRE) have been extensively studied, serological diagnostic tools for these organisms are lacking. The VanA and VanB classes of VRE show marked resistance. Here, we identified the VanA and VanB proteins that are immunogenic in mice...
January 8, 2018: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/29309212/development-of-bead-based-multiplexed-immunoassay-for-evaluation-of-midkine-syndecan-1-and-angptl4-in-patient-serum
#12
Imad Tarhoni, Cristina L Fhied, Mark Pool, Michael J Liptay, Philip Bonomi, Christopher W Seder, Jeffrey A Borgia
BACKGROUND: Angiogenesis is associated with tumor progression in a range of malignancies. Herein, we develop custom immunobead assays for several mechanistically important targets and evaluated these against sera from cohorts of non-small cell lung cancer (NSCLC) patients. METHODS: Antigen "capture" antibodies for midkine, syndecan-1, and ANGPTL4 were independently conjugated to MagPlex® Microspheres using standard carbodiimide/NHS-based chemistry. These reagents served as the basis for quantitative sandwich assay assembly using biotinylated detection antibodies and R-phycoerythrin-conjugated streptavidin reporter system...
2018: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/29308976/about-molecular-profile-of-lung-cancer-in-tunisian-patients
#13
Faouzi Mezni, Mona Mlika, Hamouda Boussen, Habib Ghedira, Soraya Fenniche, Talmoudi Faten, Marie-Anne Loriot
BACKGROUND: Molecular profile of lung cancer is well known in developed countries. These countries reached the era of liquid biopsies, immunotherapy, and urine circulating tumor DNA. The discrepancies between developed countries and developing ones are becoming deeper. Because of a lack of data in Tunisia, we tried to analyze the molecular profile of non-small-cell carcinomas and to assess the morphologic subtype of adenocarcinomas according to their mutational profile. METHODS: We performed molecular analyses in Tunisia and in France of 84 patients who were able to afford the cost of the diagnostic techniques carcinomas diagnosed between 2012 and 2015...
2018: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/29308973/current-and-emerging-egfr-therapies-for-glioblastoma
#14
REVIEW
Stefan Alexandru Artene, Cristian Tuţă, Alexandra Dragoi, Oana Alexandru, Purcaru Stefana Oana, Daniela Elise Tache, Maria Mihaela Dănciulescu, Mihai Virgil Boldeanu, Cristian Adrian Siloşi, Anica Dricu
Glioblastomas (GBMs) are the most lethal and hard to treat malignancies in clinical practice. The standard of care for treating GBM involving surgery and adjuvant radiotherapy and concomitant temozolomide (TMZ) has remained virtually unchanged in the past decade. Molecular targeted therapies against cancer-specific structures have reported mediocre results in the treatment of GBM, due to multiple factors such as the presence of the blood brain barrier or a vast array of molecular alterations which greatly hinder the action of the most therapeutic agents...
2018: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/29244613/kinetics-study-on-recombinant-alkaline-phosphatase-and-correlation-with-the-generated-fluorescent-signal
#15
Vanessa Susini, Veronica Lucia Rossi, Antonio Sanesi, Laurent Drazek
Alkaline phosphatase (AP) (EC 3.1.3.1) is one of the most commonly used enzymes in immunoassays. In VIDAS® assays (bioMérieux, Marcy l'Etoile, France), AP catalyzes the hydrolysis of 4-methylumbelliferyl phosphate (4-MUP) in 4-methylumbelliferone (4-MU) producing a fluorescent signal. This work introduces an original method of characterization of the kinetic parameters Km , Vmax, and Kcat of AP embedded in VIDAS® assays. Assessment of such constants allows us to predict the fluorescent signal generated for given amounts of enzyme and its associated substrate; in the particular case of VIDAS®, it has been estimated that 0...
2018: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/29144206/c-jun-expression-in-lichen-planus-psoriasis-and-cutaneous-squamous-cell-carcinoma-an-immunohistochemical-study
#16
Asmaa Gaber Abdou, Alaa Hassan Marae, Mohammed Shoeib, Ghada Dawood, Enas Abouelfath
The AP-1 transcription factor complex is a key player in regulating inflammatory processes, cell proliferation, differentiation, and cell transformation. The aim of the present study is to investigate C-Jun (one of AP-1complex) expression and its proliferative role in skin samples of lichen planus, psoriasis as common inflammatory skin diseases and squamous cell carcinoma using immunohistochemical method. The present study was carried out on skin biopsies of 15 psoriatic patients, 15 lichen planus patients, 15 SCC, and 15 normal skin biopsies...
2018: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/29144198/development-of-novel-fluorescent-particles-applicable-for-phagocytosis-assays-with-human-macrophages
#17
Cecilia Sóñora, Paula Arbildi, Iris Miraballes-Martínez, Ana Hernández
Phagocytosis is a fundamental process for removal of pathogens and for clearance of apoptotic cells. The objective of this work was the preparation of fluorescent microspheres by a simple method and the evaluation of its applicability in phagocytosis assays by using different human derived cells, differentiated THP-1 cell line and blood monocytes, with flow cytometry measurements for functionality assays. Our results show that microparticles are efficiently internalised in a non-opsonised form and in dose-dependent manner by both cellular types...
2018: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/29144197/evaluation-of-umelisa%C3%A2-t4-neonatal-and-umelisa%C3%A2-t4-using-polystyrene-plates-coated-with-anti-thyroxine-t4-monoclonal-antibodies
#18
Elisa M Castells, Ernesto C González, Pedro L Pérez, Lesley Del Río, Yileidis Tejeda, Yenitse Perea, Odalys Martín, Maryeris Espinosa, Jose A Rivero, Amarilys Frómeta
Congenital hypothyroidism is one of the most common preventable causes of mental retardation. The Center of Immunoassay has developed the UMELISA® T4 NEONATAL and UMELISA® T4 to determine neonatal T4 levels in dried blood and serum samples. Both reagent kits use the same polystyrene plates coated with anti-thyroxine (T4) polyclonal antibodies as solid phase. This work shows the re-standardization of the UMELISA® T4 NEONATAL and UMELISA® T4 using plates coated with anti-T4 monoclonal antibodies (T4Mabs)...
2018: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/29144195/extraction-purification-of-prostate-specific-antigen-psa-and-establishment-of-radioimmunoassay-system-as-a-diagnostic-tool-for-prostate-disorders
#19
Ahmed Sami Abu-Bakr El-Bayoumy, Akaber Tarek Hessien Keshta, Khaled Mohamed Sallam, Nahed Hassan Ebeid, Hatem Mohamed Elsheikh, Bsheer El-Sayed Bayoumy
This study aimed to provide an easy and effective method for extraction and purification of prostate-specific antigen (PSA) from human seminal fluid with high quantity (14 mg) and high purity (98%). The obtained PSA was injected into rabbits for production of anti-PSA polyclonal antibody (titer 1/1000), labeled with radioactive iodine-125 for preparation of radioactive PSA tracer (purity 98 ± 1.8% and specific activity 64 ± 1.9 µCi/µg), and used in preparation of PSA standards. All prepared components can be used in PSA immunoassays specially radioimmunoassay (RIA) kit preparation as a diagnostic tool for prostatic diseases...
2018: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/29144192/seeding-of-the-mucosal-leukocytes-in-the-halt-and-trachea-of-white-leghorn-chickens
#20
Madelyn Krunkosky, Maricarmen García, Leticia Gabriela Beltran Garza, Ebru Karpuzoglu-Belgin, Jaclyn Levin, Robert J Williams, Robert M Gogal
Characterising the immune cells of the head-associated lymphoid tissues (HALT) and trachea during maturation in young birds is critical to understanding the immunological responses to avian respiratory diseases and vaccines. Selected mucosal leukocytes of the conjunctiva-associated lymphoid tissue (CALT), Harderian gland (HG), nasal-associated lymphoid tissue (NALT) and trachea from 4-, 6-, 8-, and 10-week-old chickens were enumerated and phenotyped. HG, NALT, and trachea cellularity increased as the birds aged with cell viability varying by tissue...
2018: Journal of Immunoassay & Immunochemistry
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