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Journal of Immunoassay & Immunochemistry

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https://www.readbyqxmd.com/read/28640738/evaluation-of-the-effect-and-mechanism-of-action-of-local-phenytoin-in-treatment-of-vitiligo
#1
Asmaa Gaber Abdou, Mohammed Abdelwahed Gaber, Nada Farag Elnaidany, Ayat Elnagar
There are many theories explaining vitiligo such as genetic, autoimmune, neural, free radicals, biochemical, intrinsic defect, melanocytorrhagy and convergent theories. Phenytoin is a widely used anticonvulsant, which is used in cutaneous medicine for treatment of ulcers and epidermolysis bullosa. The aim of this study is to assess the effectiveness of topical phenytoin gel in treatment of vitiligo patients and explaining the underlying mechanism using immunohistochemistry for evaluation of HMB45, CD4 and CD8...
June 22, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/28617178/concentrations-of-pneumococcal-iga-and-igm-are-compromised-in-some-individuals-with-antibody-deficiencies
#2
Ainara Echeverría de Carlos, Ricardo Gómez de la Torre, Enrique García Carus, Luis Caminal Montero, Jose Bernardino Díaz López, Hector Suárez Casado, Luis Molinos Matin, Lourdes Tricas Aizpún, Stephen Harding, Antony R Parker
The response to pneumococcal vaccination is assessed by measurement of antigen specific IgG only and is compromised in a number of antibody deficiencies. We measured the concentrations of Pneumococcal IgA and IgM in individuals with both normal and abnormal pneumococcal capsular polysaccharide (PCP) IgG concentrations. A higher number of individuals had abnormal pre vaccination IgA and IgM concentrations below the lower limit of the normal range compared to the control group. Post vaccination a lower number of individuals had IgA and IgM concentrations below the upper limit of the normal range compared to the control group...
June 15, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/28613137/measurement-of-the-igg2-response-to-pneumococcal-capsular-polysaccharides-may-identify-an-antibody-deficiency-in-individuals-referred-for-immunological-investigation
#3
Antony Parker, Juan Irure Ventura, Dawn Sims, Ainara Echeverría de Carlos, Ricardo Gómez de la Torre, Lourdes Tricas Aizpún, J Gonzalo Ocejo-Vinyals, Marcos López-Hoyos, Gregg Wallis, Stephen Harding
IgG2 is the most efficient subclass for providing protection against pneumococcal pathogens. We hypothesised that some individuals may be unable to mount an effective pneumococcal capsular polysaccharide (PCP) IgG2 response despite having a normal PCP IgG concentration (PCP IgG2 deficient). The median pre-vaccination PCP IgG2 concentration was significantly lower in individuals referred for immunological investigation compared to healthy controls (2.8 mg/L range, 95% CI 1.1-88 vs 29.5mg/L, 95% CI 13.5-90, p=0...
June 14, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/28613106/innovative-array-based-assay-for-omics-pattern-in-melanoma
#4
Carmen Dumitru, Carolina Constantin, Cristiana Popp, Mirela Cioplea, Sabina Zurac, Tatiana Vassu, Monica Neagu
Cutaneous melanoma remains a major health issue and still an important challenge for research. Thus omics complex evaluation can provide a more specific molecular classification for this heterogeneous disease. Complex omics analysis based on genomic and proteomic microarrays can identify disease markers that prognosticate disease evolution or can monitor therapies efficacy. Among the technologies that gained momentum in the last years, array based comparative genomic hybridization offered the possibility to analyze chromosomal numerical aberrations within cutaneous melanomas providing important support for molecular classification of melanoma tumors...
June 14, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/28548901/neuroendocrine-differentiation-in-basal-cell-carcinoma
#5
Yoldez Houcine, Ines Chelly, Alia Zehani, Linda Belhaj Kacem, Haifa Azzouz, Wafa Rekik, Hend C, Slim Haouet, Nidhameddine Kchir
Basal cell carcinoma (BCC) is the prototypical basaloid tumor of the skin. It may show various patterns simulating other cutaneous tumors due to its pleomorphism. It may have an unusal pattern of differentiation such as squamous, sebaceous, apocrine, eccrine, pilar, and endocrine differentiation. In order to establish the relative frequency of neuroendocrine differentiation in BCC, we performed a retrospective study of 33 consecutive BCCs using conventional immunohistochemistry with two neuroendocrine antibodies: Chromogranine A and synaptophysine...
May 26, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/28537478/preparation-and-development-of-solid-phase-radioimmunoassay-system-for-determination-of-cortisol
#6
Kh M Sallam
The goal of this article was oriented to prepare stable polystyrene coated tubes for direct radioimmunoassay (RIA) of human cortisol. Coating process was performed using rabbit polyclonal antisera specific for cortisol. The stability study showed that these tubes could be stored for up to one year at 4ºC without any appreciable reduction in their binding. Labeling of cortisol was carried out using chloramin-T method and the (125)I-cortisol tracer was purified using HPLC column. Preparation of polyclonal antibodies (anti-cortisol) was carried out in host rabbit animals against cortisol-21-hemisuccinate:Bovine serum albumin conjugate...
May 24, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/28537469/improvement-of-elisa-procedures-through-simultaneous-addition-of-antigen-and-detection-antibody-and-elimination-of-washing-steps
#7
V Gurevich, P Kotharu, K McCann, J Bertolini
The enzyme-linked immunosorbent assay (ELISA) is extensively used for measurement of proteins utilised for various research and diagnostic purposes in the biological disciplines. However it is a labour intensive and lengthy procedure due to a number of incubation and washing steps required for performing the assay. The ELISA procedure in the current study has been simplified through the simultaneous addition of antigen and detection antibody and elimination of washing steps. This resulted in a decreased time required to perform the procedure and without affecting assay capability...
May 24, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/28521601/significance-of-pregnancy-test-false-negative-results-due-to-elevated-levels-of-%C3%AE-core-fragment-hcg
#8
Sarah Johnson, Saji Eapen, Peter Smith, Graham Warren, Michael Zinaman
Very high levels of β-core fragment human chorionic gonadotrophin (βcf-hCG) are reported to potentially cause false negative results in point-of-care (POC)/over-the-counter (OTC) pregnancy tests. To investigate this further, women's daily early morning urine samples, collected prior to conception and during pregnancy, were analysed for intact, free β-, and βcf-hCG. The proportion of βcf-hCG was found to be related to that of hCG produced and in circulation. Therefore, best practice for accuracy testing of POC/OTC pregnancy tests would be to test devices against clinical samples containing high levels of βcf-hCG as well as standards spiked with biologically relevant ratios...
May 19, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/28475466/an-immunochromatographic-assay-for-rapid-etection-of-salvinorin-a
#9
Madan Kumar Paudel, Osamu Shirota, Seiichi Sakamoto, Satoshi Morimoto, Hiroyuki Tanaka
We developed an immunochromatographic assay (ICA) that enables rapid analysis of salvinorin A (Sal A) in Salvia divinorum within 10 min. The result shows that no Sal A in other samples of Lamiaceae plants was detected, but it could recognize Sal A among other substances in complex samples. The main advantage of the ICA is its high performance in combination with low cost, simplicity, and speed. Our newly developed combined ICA/indirect competitive ELISA(icELISA) system enables analysis of large numbers of samples over short periods of time without cumbersome pretreatments in complex mixtures...
May 5, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/28422565/ascorbic-acid-deficiency-in-patients-with-lichen-planus
#10
Ilinca Nicolae, Cristina Iulia Mitran, Madalina Irina Mitran, Corina Daniela Ene, Mircea Tampa, Simona Roxana Georgescu
OBJECTIVES: Recent studies have highlighted the role of oxidative stress in the pathogenesis of lichen planus (LP). In the present study, the interest of the authors is focused on the investigation of ascorbic acid status in patients with LP and identification of parameters that might influence the level of this vitamin. MATERIAL AND METHOD: We analyzed the level of urinary ascorbic acid (reflectometric method) in 77 patients with LP (cutaneous LP (CLP)-49 cases; oral LP (OLP)-28 cases) and 50 control subjects...
April 19, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/28422564/evaluation-of-two-in-house-immunoenzymatic-tests-to-serodiagnose-subclinical-paratuberculisis-in-a-sheep-flock-in-mexicali-valley-mexico
#11
Elizama Ponce Barraza, Tomás Cárdenas Reyna, Carlos Angulo, José Carlomán Herrera Ramírez, Gilberto V López, Gerardo Enrique Medina-Basulto, Shigetoshi Eda, Sawako Hori-Oshima
Paratuberculosis (PTB) or Johne's disease is a common ruminant infectious disease caused by Mycobacterium avium subsp. paratuberculosis (MAP). In this study, two MAP antigens were compared for their diagnostic utility to detect subclinical PTB in a sheep flock in Mexicali, Mexico. Sheep (n = 31) without clinical signs but positive on a direct fecal-polymerase chain reaction were tested with two preabsorbed in-house enzyme linked immunosorbent assays (ELISAs) using: (1) an ethanol-extracted surface lipid antigen (EVELISA) and (2) a protoplasmic antigen (ELISA-PPA)...
April 19, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/28421869/alk-protein-expression-in-pulmonary-adenocarcinoma-of-tunisian-patients
#12
Amira Toumi Arfaoui, Ahlem Blel, Raoudha Aloui, Ghaya Fatnassi, Jihane Ben Ammar, Nadia Znaidi, Yosra Zidi, Mohammed Sadok Boudaya, Hichem Aouina, Soumaya Rammeh Rommani
BACKGROUND: It is now necessary to determine ALK status in order to use targeted therapy. AIM: herein, we assess immunohistochemical profile of ALK protein in a series of Tunisian patients with pulmonary adenocarcinoma. MATERIALS AND METHODS: ALK protein expression was studied applying the D5F3 antibody with a fully automated Ventana CDx technique on a series of 19 patients. RESULTS: Positive ALK expression was found in one case (5...
April 19, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/28421864/selfie-autoimmunity-boon-or-bane
#13
Haseeb Ahsan
The immune system provides protection to tissues damaged by infectious microrganisms or physical damage. In autoimmune diseases, the immune system recognizes and attacks its own tissues, i.e., self-destruction. Various agents such as genetic factors and environmental triggers are thought to play a major role in the development of autoimmune diseases. A common feature of all autoimmune diseases is the presence of autoantibodies and inflammation, including mononuclear phagocytes, autoreactive T lymphocytes, and autoantibody producing B cells (plasma cells)...
April 19, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/28318369/high-rate-of-hepatitis-b-virus-infection-among-hairdressers-in-ibadan-nigeria
#14
B A Olusola, E A Gometi, O Ogunsemowo, D O Olaleye, G N Odaibo
Hepatitis B virus (HBV) infection is a major public health problem for over two billion people infected globally. Occupationally exposed persons are at high risk of HBV infection and, apart from medical personnel, there is dearth of information concerning the prevalence and awareness of HBV among this population in Nigeria. This study was designed to determine the levels of HBV awareness and prevalence of HBV infection among hairdressers in Ibadan, Nigeria. Hairdressers and teachers (unmatched controls) in four local government areas in Ibadan were tested for HBV infection using ELISA technique...
March 20, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/28436708/a-novel-in-vitro-elisa-for-estimation-of-glycoprotein-content-in-human-rabies-vaccines
#15
Shukra M Aavula, Gontu Abhinay, Sridevi V Nimmagadda, Kapil Maithal
In vitro methods for quantification of immunodominant glycoprotein in the rabies vaccine formulations serve as good alternative to the cumbersome and variable mice potency assay as a batch release test for the vaccine. The present study presents the development of a sandwich ELISA with optimal concentrations of a high affinity recombinant diabody (D06) and a specific monoclonal antibody (M5B4) against rabies glycoprotein for its quantification in the vaccine formulations. The glycoprotein estimate correlated linearly (r(2) = 0...
February 15, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/28375073/myositis-non-inflammatory-mechanisms-an-up-dated-review
#16
Emilia Manole, Alexandra E Bastian, Niculina Butoianu, Hans H Goebel
Idiopathic inflammatory myopathies (IIM) represent a heterogeneous group of rare muscular diseases, with no clearly known causes. IIM frequently have an incomplete response to treatment due to the difficulty in distinguishing between IIM forms, and due to neglect their non-inflammatory causes. Important data concerning non-immune mechanisms in IIM pathology have been recently accumulated. There is a correlation between inflammatory and non-inflammatory mechanisms, but their involvement in IIM pathogenesis is still unknown...
2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/28102778/highlights-of-new-immunoassay-based-technologies
#17
EDITORIAL
Cristiana Tanase, Radu Albulescu, Monica Neagu
No abstract text is available yet for this article.
2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/27892811/simultaneous-determination-of-glycyrrhizin-and-liquiritin-in-licorice-roots-and-kampo-medicines-by-combination-enzyme-linked-immunosorbent-assay-using-anti-glycyrrhizin-and-anti-liquiritin-monoclonal-antibodies
#18
Shunsuke Fujii, Osamu Morinaga, Takuhiro Uto, Shuichi Nomura, Yukihiro Shoyama
Immunoassay systems using monoclonal antibodies (mAbs) are one of the most useful techniques in the analytical, biochemical, and clinical fields. In this study, a combination enzyme-linked immunosorbent assay (ELISA) using both anti-glycyrrhizin and anti-liquiritin mAbs (anti-GL/Liq mixture mAbs) was developed for quality control of licorice and its products. The combination ELISA demonstrated high sensitivity, reproducibility, and specificity for the total content of GL and Liq by a single assay. The developed ELISA was effective and useful as the first screening method in the selection of high-quality licorice from the Glycyrrhiza species and in confirming the quality of licorice-containing Kampo medicines...
2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/27797300/a-new-covalent-peroxidase-conjugation-method-using-bis-sulfosuccinimidyl-suberate-as-cross-linking-reagent-in-a-two-step-procedure
#19
Rivo Presentini
A new method has been developed to prepare horseradish peroxidase (HRP) conjugates using bis(sulfosuccinimidyl)suberate (BS3) as cross-linking reagent in a two-step procedure. The enzyme is reacted with BS3, introducing active ester molecules into the enzyme itself, and it is then used directly to label amino-containing compounds without further treatment. The proteins involved in the conjugation undergo minimal modifications. The reaction conditions are evaluated, as well as studies on the conservation of the biological activities of the conjugated proteins and the stability of the conjugates in time...
2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/27624975/selection-of-specific-inhibitor-peptides-in-enzyme-linked-immunosorbent-assay-elisa-of-cardiac-troponin-i-using-immuno-dominant-epitopes-as-competitor
#20
Majid Asiabanha Rezaee, Mohammad Javad Rasaee, Javad Mohammadnejad
Human cardiac troponin I (cTni) is the gold marker for early diagnosis of myocardial infarction. In this regard, four immune-dominant epitopes of cTni were predicted and their 3D structures were determined. Thereafter, the competitive performance of the peptides was monitored with the developed polyclonal antibody-based indirect competitive ELISA; a half-maximal inhibitory concentration (IC50) of 0.49 (µg/mL) and detection limit of 0.037 (µg/mL) were achieved for recombinant cTni. The competitive ELISA determined sensitivity levels of 0...
2017: Journal of Immunoassay & Immunochemistry
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