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Journal of Immunoassay & Immunochemistry

Oladipo Elijah Kolawole, Awoyelu Hilda Elukunbi, Oloke Julius Kola
Infection caused by Human parvovirus B19 (HPVB19) during pregnancy has been associated with adverse effects on fetus such as intrauterine fetal death but there is dearth information about the seroprevalence and risk factors among pregnant women. This study was carried out to determine the incidence of specific IgM antibodies to HPVB19 among pregnant women attending Bowen University Teaching Hospital, Ogbomoso, Oyo State. A total of 185 participants, in the age range 18-49 years, who consented voluntarily after thorough explanation of the purpose of the study were recruited for the study...
September 13, 2018: Journal of Immunoassay & Immunochemistry
Reham F El-Kased
Cardiac troponin-I is a promising diagnostic marker for cardiovascular diseases. Troponin-I immunoassays rely on monoclonal antibodies, while polyclonal antibodies, cheaper to manufacture, are uncommonly used. The current study established an immuno-analytical assay using a polyclonal antibody capable of mapping troponin-I antigenic determinant. Proteolytic digestion of troponin-I was performed. Antigenic determinant was assigned by separation of fragments using gel electrophoresis followed by Western blot and high-performance liquid chromatography followed by dot blot...
September 13, 2018: Journal of Immunoassay & Immunochemistry
Richard Edem Antia, Adewole Augustine Adekola, Afusat Jagun Jubril, Obokparo Godspower Ohore, Benjamin Obukowho Emikpe
Hepatitis E (HE) is an important viral hepatitis of global public and livestock health, and food security significance due to the ubiquitous distribution, multiple transmission route and zoonotic potentials. HE is also endemic in most developing countries including Nigeria. This study therefore investigates the seroprevalence and associated risk factors of HEV infection in pigs raised in Ibadan, Nigeria. Taking an analytical cross-sectional study design, 176 animals (comprising 120 pigs, 26 goats, and 30 cattle) were randomly sampled at the Bodija Municipal abattoir, Ibadan...
September 13, 2018: Journal of Immunoassay & Immunochemistry
Luis Carlos Ortiz-Dosal, Gabriela Ángeles-Robles, Eleazar Samuel Kolosovas-Machuca
Hafnium(IV) oxide is a material with properties that can increase the sensitivity, durability, and reliability of biosensors made from silicon dioxide and other semiconductor materials due to its high dielectric constant, thermodynamic stability, and the simplicity with which it can be deposited. This work describes the use of this material in biosensors based on field-effect transistors to detect ions and DNA, in immunosensors to detect an antigen-antibody complex, its use as a contrast material in computed tomography scans and the possibility of using it in optic biosensors in the infrared region...
September 6, 2018: Journal of Immunoassay & Immunochemistry
Asmaa Gaber Abdou, Moshira Abdelwahed, Abdelnabei Said, Doha Maher Taie, Sara Fahmy
Papillary thyroid carcinoma (PTC) is diagnosed in both cytological and histological specimens on the basis of distinct nuclear morphology. These features may not be prominent in some PTC variants and may be seen in some benign conditions. It is necessary to differentiate PTC from other neoplastic and nonneoplastic lesions since it affects treatment strategy and patients' fate. Emerin is a type II integral membrane protein of the inner nuclear membrane that has a characteristic staining pattern in PTC. CD56 is a homophilic membrane glycoprotein that is expressed in thyroid follicular epithelial cells and adrenal glands...
September 6, 2018: Journal of Immunoassay & Immunochemistry
Reham Sabry, Zakia Abu Zahab Mohamed, Amany M Abdallah
Hepatitis B virus (HBV) vaccination leads to both humoral and cellular immune responses and results in protecting levels of specific antibodies. The immune response to the vaccine and production of T-helper 1 (Th1) and Th2 cytokines vary among the individuals. Thus we aimed to investigate the relationship between Th1/Th2 cytokine serum levels and antibody production after HBV vaccination, hoping to improve the effectiveness of vaccination. A total of 90 health care workers had been vaccinated with triple doses of HB vaccine at 0, 1, and 6 months intervals...
September 5, 2018: Journal of Immunoassay & Immunochemistry
Ashkan Pirayesh, Samira Ferdosi, Hedayatollah Shirzad, Soroush Amani, Hajar Bahadivand Chegini, Nader Bagheri, Ladan Sadeghian, Farbod Torkamand
BACKGROUND AND AIMS: This study aimed to assess the level of the expression of CCL-18 on nasal inferior turbinate mucosa in patients with mild (M) and moderate-to-severe (M/S) persistent allergic rhinitis (PAR). METHODS: The participants in this case-controlled study were divided into three groups: patients with M/S PAR, patients with M PAR, and the healthy control group. Biopsies of nasal inferior turbinate mucosa were obtained from the participants. Expression of CCL-18 mRNA was evaluated by real-time PCR...
August 13, 2018: Journal of Immunoassay & Immunochemistry
Yiling Gao, Xinxin Huang, Yongbo Zhu, Zhiyuan Lv
Modern immunoassay methods and techniques are important tools in labs of basic biology, biomedicine, clinical medicine, and even in home tests, such as pregnant test, many of which utilize antibody-antigen binding mechanism as their foundational principle in common. Meanwhile, compared with polyclonal anitbody, monoclonal antibody shows obvious advantages in their application of modern immunoassays. Furthermore, the progress of other technologies have also promoted the development of modern immunoassays robustly, and widely made it extend into more research and industry fields...
2018: Journal of Immunoassay & Immunochemistry
P Das, J Mohanty, M R Badhe, P K Sahoo, K K Sardar, S C Parija
A study was undertaken to develop a western blot method for detection of immunogenic proteins of fish ectoparasite, Argulus siamensis for its further use as potential vaccine candidates. Argulus antigens were prepared by homogenization and injected to rohu (Labeo rohita) juveniles for development of immune serum. The serum was used to immunostain the antigens in western blot. The other reagents added in sequence were rabbit anti-rohu serum, goat anti-rabbit ALP conjugate and substrate (BCIP-NBT). However, similar banding patterns were observed with both control and immunized rohu serum...
2018: Journal of Immunoassay & Immunochemistry
Emiliana Mendoza, José Bubis, Yenis Pérez-Rojas, Alejandro J Montilla, Lilian M Spencer, Floritza Bustamante, Juan C Martínez
Previously, we have identified a protein in Trypanosoma equiperdum that possesses homology with the regulatory (R) subunits of the mammalian cAMP-dependent protein kinase (PKA). The recombinant T. equiperdum PKA R-like protein was expressed in bacteria and purified to homogeneity. Mice polyclonal antibodies were raised against the recombinant R-like protein to serologically evaluate its humoral immune response. High titers of specific sera antibodies were obtained against the parasite R-like protein by indirect enzyme-linked immunosorbent assay (ELISA), and immunoblots revealed that this protein was specifically recognized by the hyperimmune mice sera...
2018: Journal of Immunoassay & Immunochemistry
Augustine Okechukwu Udeze, Mutiat B Odebisi-Omokanye, Adedayo Faneye, Babatunde Adebiyi Olusola, Olukunle Ogunsemowo, Cecilia Iwuoha, Victoria Atoyebi
Human T-cell lymphotropic virus types I/II (HTLV-I/II) is endemic in some parts of the world including Nigeria. Reported prevalence rates in Nigeria have largely focused on blood donors. This study aims at determining the prevalence of HTLV infection among pregnant women in Ilorin North-central Nigeria. Serum samples from 276 pregnant women who were antenatal clinic attendees at General and Civil Service Hospitals in Ilorin were tested for the presence of HTLV-I/II antibodies using Enzyme Linked Immunosorbent Assay test kits from Diagnostic Automation INC...
2018: Journal of Immunoassay & Immunochemistry
Natália Maria Lanzarini, Gentil Arthur Bentes, Eduardo de Mello Volotão, Marcelo Alves Pinto
Immunoglobulin Y (IgY), an antibody present in birds, reptiles, and amphibians, is actively transported from the serum to egg yolks, where it is stored in large quantities. The use of chicken polyclonal IgY instead of mammalian IgG antibodies for biomedical applications has ethical and economic advantages, such as the lack of a need for animal bleeding because the antibodies are extracted from eggs after hen immunization and the low cost of the production and purification methods. This article reviews the latest IgY applications in diagnostic virology and the therapeutic use of IgY in viral gastroenteritis...
2018: Journal of Immunoassay & Immunochemistry
Adeleye Solomon Bakarey, Inioluwa Oyindamola Akinboade, Yetunde Adebisi Aken'Ova
INTRODUCTION: Hepatitis B virus infection attacks the liver and can cause both acute and chronic disease. Sickle cell disease (SCD) patients are at risk of transmission transmissible viral hepatitis due to their constant need for blood transfusion. However, these patients could have been infected with HBV but may not know their status due to asymptomatic nature of the infection. Therefore, this study was designed to determine the burden of HBV markers of infection among SCD patients attending the hematology clinic at a tertiary health facility in Ibadan, Nigeria...
2018: Journal of Immunoassay & Immunochemistry
Abosede Yetunde Afolabi, Adeleye Solomon Bakarey, Oladipo Elijah Kolawole, Oloke Julius Kola
BACKGROUND: Over 90% of infant acquired immunodeficiency syndrome (AIDS) cases have been through mother-to-child transmission (MTCT) of human immunodeficiency virus (HIV). Consequent to this, prevention of mother-to-child transmission (PMTCT) programs have instituted as dual purposes for prevention of HIV transmission from mother to child and enrollment of infected pregnant women and their families into antiretroviral treatment (ART) program. However, there are still some breakthrough infections and challenges...
2018: Journal of Immunoassay & Immunochemistry
Adeola Fowotade, Deborah Afekelu Osisanwo, Rasheed Ajani Bakare
Infection with high-risk genotypes of human papillomavirus (HPV) is considered the main cause of invasive cervical. A number of epidemiologic studies have suggested that HPV and Chlamydia trachomatis (CT) play a synergistic role in the etiology of cervical intraepithelial neoplasia and subsequent cervical cancer. The current study aimed to evaluate the HPV prevalence and the risk factors for co-infection with CT among women attending family planning clinic in Nigeria. Following enrolment, 90 patients were screened for IgG antibodies to virus-like proteins of HPV types 6, 8, 16, and 18...
2018: Journal of Immunoassay & Immunochemistry
Luu Manh Quynh, Chu Tien Dung, Bach Thi Mai, Hoang Van Huy, Nguyen Quang Loc, Nguyen Quang Hoa, Pham Tuan Thach, Bui Viet Anh, Chu Thi Thao, Nguyen Hoang Nam, Hoang Thi My Nhung, Nguyen Ngoc Long, Le Van Vu
Fe3 O4 /Ag core/shell nanoparticles functionalized with the free amino (NH2 ) functional groups (Fe3 O4 /Ag-NH2 ) were conjugated with fluorescent electron coupled dye (ECD)-antiCD34 antibody using the 1-ethyl-3-(3'-dimethyl-aminopropyl) carbodiimide (EDC) catalyst (ECD - Electron Coupled Dye or R Phycoerythrin-Texas Red is a fluorescent organic dye attached to the antibody). The characteristic fluorescence of ECD in the antibody was investigated and was used as a good indicator for estimating the percentage of the antibodies that were successfully conjugated with the nanoparticles...
2018: Journal of Immunoassay & Immunochemistry
Mohamed M Omran, Faten M Zahran, Mohamed Kadry, Arafa A M Belal, Tarek M Emran
Myeloperoxidase (MPO) is an inflammatory marker, elevated in acute coronary syndromes (ACSs), especially in acute myocardial infarction (AMI) cases. This study aimed to evaluate the diagnostic power of MPO in AMI patients. MPO, creatine kinase (CK) MB, and Troponin I (cTn I) were performed for all study patients. Area under the curves (AUCs) and 95% confidence intervals (CI); P values of baseline levels of MPO for discriminating AMI patients from noncoronary chest pain (NCCP) patients, stable angina (SA) patients, and unstable angina (UA) patients were 0...
2018: Journal of Immunoassay & Immunochemistry
Sasiwan Kerdpoo, Witida Laopajon, Watchara Kasinrerk, Supansa Pata, Thanusak Tatu
Hemoglobin F (HbF) in blood lysate can be accurately measured by various methods, including immunoassay. In this study, we have produced polyclonal antibody (pAb) against HbF and established a modified sandwich-type ELISA for HbF quantification in blood lysates. The modified sandwich ELISA utilized anti-γ-globin monoclonal antibody clones Thal N/B as the capture antibody (Ab) coated on solid-phase, fluorescein isothiocyanate (FITC)-labeled pAb as the detecting Ab, and HPR-labeled anti-FITC Ab as the signal-generating Ab...
2018: Journal of Immunoassay & Immunochemistry
Temsiri Songjaroen, Vitsarut Primpray, Thawintra Manosarn, Witsanuwat Khumchanta, Tasanee Sakuldamrongpanich, Suntree Kulkeratiyut, Wanida Laiwattanapaisal
ABO blood group is the most important blood type system for transfusion medicine. A paper-based analytical device (PAD) for ABO blood typing has been proposed. The device was composed of Whatman No. 113 paper, an absorbent gel pad, and a 3D-printing cassette. The 3D-printing cassette contained two circular holes for display of letters "A" and "B" on the PAD. Whole blood was dropped onto hydrophilic letters A and B on the PAD, in which the anti-A and anti-B were pre-immobilized, respectively...
2018: Journal of Immunoassay & Immunochemistry
Alice Grigore, Adrian Albulescu, Radu Albulescu
Oncoimmunology is a rapidly growing field, focusing both on studying of the interaction of immune factors with tumor cells and also on the development of new therapies. In this regard, immunotherapy is increasingly used clinically. Although tumorigenesis is generally seen as an autonomous process involving genetically transformed cancer cells, it is increasingly recognized that tumor environment is an essential factor that modulates both tumor progression and resistance to therapy. Tumor-associated immune cells, and in particular macrophages, are of particular importance in all stages of the tumorigenesis process and are also a clinical prognostic marker...
2018: Journal of Immunoassay & Immunochemistry
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