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Journal of Immunoassay & Immunochemistry

Nazila Ariaee, Abdolreza Varasteh, Farahzad Jabbari Azad, Mojtaba Sankian
Atopic diseases have an increasing trend worldwide during the last two decades. Determining the main cause of allergic diseases, allergens, is the first step in managing and improving the issue, usually is done by Skin Prick tests (SPTs). Having allergenic extract in high quality is desired to perform a reliable SPT. Several parameters of extracts are considered including composition, stability, potency, preservation conditions, and unit definition. In this review, these factors have been explained pointing to factors might have profitable points or harmful drawback in the quality of allergen extracts...
November 13, 2018: Journal of Immunoassay & Immunochemistry
Liliana-Roxana Balahura, Raluca-Ioana Stefan-Van Staden, Jacobus Frederick Van Staden, Hassan Y Aboul-Enein
Immunoassay technique performs a fast, simple, reliable, and sensitive analysis of different compounds, being applied in several areas of interest such as clinical analysis for medical diagnosis, as well as in environmental analysis, and food quality control. The latest research activities in this field are represented by the attempts to achieve a low limit of detection by developing of new signal amplification strategies, eliminate the interferences, and decrease the cost of analysis.
November 7, 2018: Journal of Immunoassay & Immunochemistry
Narges Farshidi, Maliheh Moghaddam, Vahid Yaghoubi, Seyed Hasan Ayati, Abdol-Reza Varasteh, Mojtaba Sankian
The incidence of grape (Vitis vinifera) allergy in the northeast of Iran is second to melon allergy. Type IV chitinase is one of the major grape allergens. The current study investigates the level of type IV chitinase in four grape variants for the first time in Khorasan Razavi Province using a highly sensitive sandwich enzyme-linked immunosorbent assay (ELISA). This assay was developed using a polyclonal antibody as a capture antibody and monoclonal antibody as a secondary one. Finally, the amount of type IV chitinase was measured by the validated ELISA test...
October 22, 2018: Journal of Immunoassay & Immunochemistry
Haseeb Ahsan
Arginine is derived from dietary intake, body protein breakdown, or endogenous de novo arginine production. Arginine methylation of non-histone proteins is used in transcriptional regulation. Protein-arginine methylation is used for regulation of transcriptional and various physiological pathological processes. Protein methylation may affect protein-protein, protein-DNA, or protein-RNA interaction. Arginine has an effect on the DNA-binding activity of NF-κB, a dominant transcriptional factor in inflammation...
2018: Journal of Immunoassay & Immunochemistry
Gnanadurai John Fletcher, Raghavendran Anantharam, Kalaivani Radhakrishnan, Unnati Singh, Amaldev Karunakaran, Visalakshi Jeyaseelan, Priya Abraham
Hepatitis B virus-e-antigen (HBeAg) is a viral marker to assess hepatitis B virus (HBV) replication. We have evaluated the reliability of three commonly available HBeAg immunoassays using World Health Organization-International Standard and clinical samples. In addition the performance of enzyme immunoassays (EIAs) was assessed by kinetic binding and reagent exchange experiments. Analytical and diagnostic sensitivity were significantly different among HBeAg assays (P < 0.01). The affinity of capture/detector antibodies varied significantly between EIAs (P < 0...
2018: Journal of Immunoassay & Immunochemistry
A V Opayele, G N Odaibo, O D Olaleye
Livestock handlers are occupationally exposed to Rift Valley fever virus (RVFV) because of frequent and longer contact with mosquito vectors, animal blood and other tissues. We determined the seroprevalence of RVF virus infection among livestock handlers in Ibadan, Nigeria. Blood samples were collected from 265 workers in two major livestock markets and a livestock farm. Questionnaires were administered to obtain information on risk factors that may be associated with RVF virus transmission. The blood samples were tested using enzyme-linked immunosorbent assays (ELISA) to detect multispecies anti-RVF virus IgG/IgM and human anti-RVF virus IgM...
2018: Journal of Immunoassay & Immunochemistry
Dayamin Martinez Brito, Elizabeth Bueno Fis, Taimi Fiallo Fernandez, Mirta Torres Castellanos, M Teresa Correa Vidal, Rodny Montes De Oca Porto
The Ultra Micro Analytical System (SUMA) is an ELISA-based analytical platform, developed andmanufactured by the Cuban Immunoassay Center (IC), which is primarily used in clinical medicine applications. In this article, we describe the validation of the UMELISA HCG kits, which are based on SUMA, as a pre-screening procedure for the detection of human Chorionic Gonadotrophin (hCG) in urine for anti-doping purposes. Validation of assay performance parameters showed satisfactory results, in accordance with the criteria established by the World Anti-Doping Agency (WADA): intra-assay repeatability (6...
2018: Journal of Immunoassay & Immunochemistry
Ali Mohammed Yousef, Sherry Kamel Abdelrahman, Ibrahim Elsayed Helmi, Fathy Mohammed Eltaweel, Walaa Elsayed Hamed
BACKGROUND: Autoantibodies are useful diagnostic tools for a variety of rheumatic autoimmune disorders. OBJECTIVE: The aim of the present study was to investigate autoantibodies against the 70-kD polypeptide of U1 ribonucleoprotein antibodies (RNP) in patients with rheumatoid arthritis (RA) and to investigate the relationship between anti-RNP and HLARD4 in RA patients. PATIENTS AND METHODS: In this prospective study, we studied 80 RA patients...
2018: Journal of Immunoassay & Immunochemistry
Liana Sticlaru, Florica Stăniceanu, Mirela Cioplea, Luciana Nichita, Alexandra Bastian, Geanina Micu, Cristiana Popp
It is suggested that different neuropeptides are actively involved in the pathogenesis of Helicobacter pylori (H. pylori)-induced gastritis acting as important effectors of the neuroimmune complex interactions, but the available data is limited and contradictory. The aim of this study was to determine whether the chronic infection generates changes in substance P (SP) and vasoactive intestinal peptide (VIP) gastric level and to evaluate the dependence of these potential effects on the degree of bacterial colonization or the severity of the inflammatory infiltrate...
2018: Journal of Immunoassay & Immunochemistry
Ramin Raoufinia, Sanaz Balkani, Neda Keyhanvar, Behroz Mahdavipor, Jalal Abdolalizadeh
BACKGROUND: Among different proteins of blood, albumin is considered a unique protein due to having special properties. Now, various protocols are used for the albumin purification worldwide, each of them has its own advantages and disadvantages. Meanwhile, a common method which is often used for the production of albumin is a combination of Cohn along with different types of chromatography. The aim of the present study was to create a concise and cost-effective albumin purification method by employing a conventional method with some modifications...
2018: Journal of Immunoassay & Immunochemistry
Mehmet Hamdi Orum, Mahmut Zabit Kara, Oguzhan Bekir Egilmez
This study was designed to compare the complete blood count (CBC) values of 38 violent suicidal attempt (VSA) and 38 nonviolent suicidal attempt (NVSA) patients and 38 healthy controls, particularly mean platelet volume (MPV) and neutrophil to lymphocyte ratio (NLR). MPV was found to be significantly higher (P = 0.000), and PLT was found to be significantly lower in the VSA group (P = 0.001). NLR was found to be significantly higher (P = 0.005) in the VSA group. The area under the receiver operating characteristic curve of MPV levels for VSA was 0...
2018: Journal of Immunoassay & Immunochemistry
Sandeep Singh, Sudhir K Dohre, Aayushi Kamthan, Vijai Pal, Bhagwan Swaroop Karothia, Hari-Sankar Singha, Subodh Kumar
Glanders is a contagious and highly fatal disease of equines with zoonotic potential. It is caused by a Gram-negative, nonmotile bacterium Burkholderia mallei. Complement fixation test (CFT) is one of the most commonly used tests for diagnosis of glanders; however, it has some limitations. A recombinant-truncated Burkholderia intracellular motility A (BimA) protein-based indirect enzyme-linked immunosorbent assay (iELISA) was previously reported by us for glanders diagnosis, which has been re-optimized in this study using a panel of glanders positive (n = 75) and glanders negative (n = 227) serum samples...
2018: Journal of Immunoassay & Immunochemistry
Iheanyi O Okonko, Amaoge Anyanwu, Anwuli U Osadebe, Ngozi N Odu
Human Immunodeficiency Virus (HIV) and tuberculosis (TB) are considered as key players in the global health scene. This study sought to determine the prevalence of TB among HIV-positive attendees of the Directly Observed Treatment of Short Course (DOTS) program at the military hospital in Port Harcourt. The association of HIV prevalence with gender and age as risk factors was also determined. A total of 200 patients aged 15-65 years were screened. Re-screening for HIV was done for confirmation. The overall prevalence for anti-TB antibodies amongst HIV-positive individuals was 14...
2018: Journal of Immunoassay & Immunochemistry
Fereshteh Alsahebfosoul, Hamidreza Jahanbani-Ardakani, Reza Ghavimi, Nahid Sedaghat, Masoud Etemadifar
INTRODUCTION: Multiple sclerosis is a chronic autoimmune demyelinating disorder of central nervous system with unknown origin. In MS disease, T cells are pointed to myelin antigens and it leads to myelin loss and axonal degeneration. Cytokines are important regulators of immune system and has critical roles in MS pathogenesis. Interleukin 36, a member of interleukin 1 family, has been shown having important roles in some autoimmune disorders due to its proinflammatory actions and its role in host immunity...
2018: Journal of Immunoassay & Immunochemistry
Oladipo Elijah Kolawole, Awoyelu Hilda Elukunbi, Oloke Julius Kola
Infection caused by Human parvovirus B19 (HPVB19) during pregnancy has been associated with adverse effects on fetus such as intrauterine fetal death but there is dearth information about the seroprevalence and risk factors among pregnant women. This study was carried out to determine the incidence of specific IgM antibodies to HPVB19 among pregnant women attending Bowen University Teaching Hospital, Ogbomoso, Oyo State. A total of 185 participants, in the age range 18-49 years, who consented voluntarily after thorough explanation of the purpose of the study were recruited for the study...
2018: Journal of Immunoassay & Immunochemistry
Reham F El-Kased
Cardiac troponin-I is a promising diagnostic marker for cardiovascular diseases. Troponin-I immunoassays rely on monoclonal antibodies, while polyclonal antibodies, cheaper to manufacture, are uncommonly used. The current study established an immuno-analytical assay using a polyclonal antibody capable of mapping troponin-I antigenic determinant. Proteolytic digestion of troponin-I was performed. Antigenic determinant was assigned by separation of fragments using gel electrophoresis followed by Western blot and high-performance liquid chromatography followed by dot blot...
2018: Journal of Immunoassay & Immunochemistry
Richard Edem Antia, Adewole Augustine Adekola, Afusat Jagun Jubril, Obokparo Godspower Ohore, Benjamin Obukowho Emikpe
Hepatitis E (HE) is an important viral hepatitis of global public and livestock health, and food security significance due to the ubiquitous distribution, multiple transmission route and zoonotic potentials. HE is also endemic in most developing countries including Nigeria. This study therefore investigates the seroprevalence and associated risk factors of HEV infection in pigs raised in Ibadan, Nigeria. Taking an analytical cross-sectional study design, 176 animals (comprising 120 pigs, 26 goats, and 30 cattle) were randomly sampled at the Bodija Municipal abattoir, Ibadan...
2018: Journal of Immunoassay & Immunochemistry
Yiling Gao, Xinxin Huang, Yongbo Zhu, Zhiyuan Lv
Modern immunoassay methods and techniques are important tools in labs of basic biology, biomedicine, clinical medicine, and even in home tests, such as pregnant test, many of which utilize antibody-antigen binding mechanism as their foundational principle in common. Meanwhile, compared with polyclonal anitbody, monoclonal antibody shows obvious advantages in their application of modern immunoassays. Furthermore, the progress of other technologies have also promoted the development of modern immunoassays robustly, and widely made it extend into more research and industry fields...
2018: Journal of Immunoassay & Immunochemistry
Luis Carlos Ortiz-Dosal, Gabriela Ángeles-Robles, Eleazar Samuel Kolosovas-Machuca
Hafnium(IV) oxide is a material with properties that can increase the sensitivity, durability, and reliability of biosensors made from silicon dioxide and other semiconductor materials due to its high dielectric constant, thermodynamic stability, and the simplicity with which it can be deposited. This work describes the use of this material in biosensors based on field-effect transistors to detect ions and DNA, in immunosensors to detect an antigen-antibody complex, its use as a contrast material in computed tomography scans and the possibility of using it in optic biosensors in the infrared region...
2018: Journal of Immunoassay & Immunochemistry
Asmaa Gaber Abdou, Moshira Abdelwahed, Abdelnabei Said, Doha Maher Taie, Sara Fahmy
Papillary thyroid carcinoma (PTC) is diagnosed in both cytological and histological specimens on the basis of distinct nuclear morphology. These features may not be prominent in some PTC variants and may be seen in some benign conditions. It is necessary to differentiate PTC from other neoplastic and nonneoplastic lesions since it affects treatment strategy and patients' fate. Emerin is a type II integral membrane protein of the inner nuclear membrane that has a characteristic staining pattern in PTC. CD56 is a homophilic membrane glycoprotein that is expressed in thyroid follicular epithelial cells and adrenal glands...
2018: Journal of Immunoassay & Immunochemistry
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