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Journal of Immunoassay & Immunochemistry

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https://www.readbyqxmd.com/read/28699851/dynamics-of-cd4-cell-count-among-hiv-infected-individuals-in-lagos-nigeria
#1
Babatunde O Motayo, Babatunde A Olusola, Adedayo O Faneye, Solomon Aturaka, Olukunle Oluwasemowo, Joseph I Ogiogwa
Human Immunodeficiency virus (HIV) infection leads to progressive loss of CD4 T- cells. Antiretroviral therapy has been able to inhibit this process, resulting in significant level of immune recovery and function. Our aim is to investigate the dynamics of CD4 recovery among HIV patients in Lagos, Nigeria. 213 HIV positive individuals were enrolled between October 2007 and May 2008, and followed up for 9 months based on CD4 count, CD4 analysis was done by flow cytometry at enrolment and after every 3 months...
July 12, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/28699839/validated-low-volume-immunoassay-for-the-reliable-determination-of-direct-renin-especially-valuable-for-pediatric-investigations
#2
J Schaefer, B B Burckhardt, J Tins, A Bartel, S Laeer
INTRODUCTION: The pharmacotherapy of pediatric patients suffering from heart failure is extrapolated from adults due to missing data in children. OBJECTIVES: Development and validation of a low-volume immunoassay for the reliable determination of renin. EXPERIMENTAL: The immunoassay was validated according to international guidelines. RESULTS: The assay allows the reliable determination of renin in 40 μL plasma within a calibration range of 4 - 128 pg/mL...
July 12, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/28699830/cag-pathogenicity-island-dependent-up-regulation-of-matrix-metalloproteinase-7-in-infected-patients-with-helicobacter-pylori
#3
Marzieh Sadeghiani, Nader Bagheri, Heshmat Shahi, Somayeh Reiisi, Ghorbanali Rahimian, Reza Rashidi, Majid Mahsa, Mohammedhadi Shafigh, Elaheh Salimi, Mahmoud Rafieian-Kopaei, Morteza Hashemzadeh-Chaleshtori, Hedayatollah Shirzad
Helicobacter pylori (H. pylori) infection has been involved in the pathogenesis of most important gastroduodenal diseases. Matrix metalloproteinases (MMPs) are a family of secreted, zinc-dependent enzymes collectively capable of degradation of extracellular matrix (ECM) and key player in various inflammatory diseases. Therefore, we examined MMP-7 mRNA levels in the gastric mucosa of patients with H. pylori infection and evaluated the effects of virulence factors, such as vacA (vacuolating cytotoxin A) and cagA (cytotoxin-associated gene) in H...
July 12, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/28692338/evaluation-of-a-novel-point-of-care-test-for-the-rapid-semi-quantitative-detection-of-allergen-specific-immunoglobulin-e
#4
René Rübenhagen, Marc Dangers
BACKGROUND: Allergen-specific IgE levels in human sera support the diagnosis of allergies. However, commonly used laboratory tests are time consuming. The new point-of-care test FastCheckPOC 20 (FCP20) allows the simultaneous and semi-quantitative evaluation of IgE antibodies for 20 aeroallergens and food allergens. METHODS: Two hundred patients with allergic symptoms were included in a multicenter performance evaluation using both whole blood and plasma or serum samples...
July 10, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/28686108/the-role-of-human-urotensin-ii-in-patients-with-hypertrophic-cardiomyopathy
#5
Saman Jumaah, Abuzer Çelekli, Murat Sucu
OBJECTIVE: Hypertrophic cardiomyopathy (HCM) is a genetic condition with the hallmark feature of left ventricular hypertrophy. Human Urotensin-II (hUT-II) is regarded as a cardiovascular autacoid/hormone, and it has cardiac inotropic and hypertrophic properties. Aims of this study were to elucidate the clinical significance of serum hUT-II levels as a potential new biomarker in patients with HCM. METHODS: This study included 40 HCM patients (60% males and 40% females) and were compared to 30 healthy control subjects (47% males and 53% females...
July 7, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/28679076/development-of-a-tree-shrew-specific-interferon-gamma-assay
#6
Xuemei Zhang, Jingwen Xu, Zhongxiang Wu, Wenbing Zhu, Shaozhong Dong
Tree shrews (Tupaia belangeri) are small squirrel-like mammals closely related to primates. Due to their susceptibility to several human viruses, tree shrews have been proposed as potential animal models for the study of human viral infections. However, there are no standardized assays currently available for the detection of tree shrew-specific interferon (IFN)-γ, a major cytokine secreted during the antiviral immune response. Herein, we developed a novel enzyme-linked immunosorbent assay (ELISA) for the quantification of IFN-γ in tree shrew serum samples...
July 5, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/28679075/first-report-of-immunohistochemical-detection-of-peste-des-petit-ruminants-parainfluenza-3-and-respiratory-syncytial-viral-antigens-in-lungs-of-nigerian-goats
#7
Theophilus A Jarikre Aghogho, Benjamin Obukowho Emikpe
This study determined the of involvement of PPR, PI3 and RS viruses in the pathology of caprine pneumonia across Nigeria. 150 goats were selected randomly. PI3 and RSV monoclonal antibodies and PPR polyclonal antibody were used for the immunolocalisation of the antigens. Histologically, 61 of the goats had broncho-interstitial pneumonia, 25 had interstitial pneumonia, 42 had bronchopneumonia, 12 had bronchiolitis and 10 were normal. PPR, PI3 and RS viral antigens were demonstrated in: intact and desquamated bronchial, bronchiolar epithelial cells, macrophages, leukocytes, pneumocytes and giant cells...
July 5, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/28640738/evaluation-of-the-effect-and-mechanism-of-action-of-local-phenytoin-in-treatment-of-vitiligo
#8
Asmaa Gaber Abdou, Mohammed Abdelwahed Gaber, Nada Farag Elnaidany, Ayat Elnagar
There are many theories explaining vitiligo such as genetic, autoimmune, neural, free radicals, biochemical, intrinsic defect, melanocytorrhagy and convergent theories. Phenytoin is a widely used anticonvulsant, which is used in cutaneous medicine for treatment of ulcers and epidermolysis bullosa. The aim of this study is to assess the effectiveness of topical phenytoin gel in treatment of vitiligo patients and explaining the underlying mechanism using immunohistochemistry for evaluation of HMB45, CD4 and CD8...
June 22, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/28617178/concentrations-of-pneumococcal-iga-and-igm-are-compromised-in-some-individuals-with-antibody-deficiencies
#9
Ainara Echeverría de Carlos, Ricardo Gómez de la Torre, Enrique García Carus, Luis Caminal Montero, Jose Bernardino Díaz López, Hector Suárez Casado, Luis Molinos Matin, Lourdes Tricas Aizpún, Stephen Harding, Antony R Parker
The response to pneumococcal vaccination is assessed by measurement of antigen specific IgG only and is compromised in a number of antibody deficiencies. We measured the concentrations of Pneumococcal IgA and IgM in individuals with both normal and abnormal pneumococcal capsular polysaccharide (PCP) IgG concentrations. A higher number of individuals had abnormal pre-vaccination IgA and IgM concentrations below the lower limit of the normal range compared to the control group. Post vaccination a lower number of individuals had IgA and IgM concentrations below the upper limit of the normal range compared to the control group...
June 15, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/28613137/measurement-of-the-igg2-response-to-pneumococcal-capsular-polysaccharides-may-identify-an-antibody-deficiency-in-individuals-referred-for-immunological-investigation
#10
Antony Parker, Juan Irure Ventura, Dawn Sims, Ainara Echeverría de Carlos, Ricardo Gómez de la Torre, Lourdes Tricas Aizpún, J Gonzalo Ocejo-Vinyals, Marcos López-Hoyos, Gregg Wallis, Stephen Harding
IgG2 is the most efficient subclass for providing protection against pneumococcal pathogens. We hypothesised that some individuals may be unable to mount an effective pneumococcal capsular polysaccharide (PCP) IgG2 response despite having a normal PCP IgG concentration (PCP IgG2 deficient). The median pre-vaccination PCP IgG2 concentration was significantly lower in individuals referred for immunological investigation compared to healthy controls (2.8 mg/L range, 95% CI 1.1-88 vs 29.5mg/L, 95% CI 13.5-90, p=0...
June 14, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/28613106/innovative-array-based-assay-for-omics-pattern-in-melanoma
#11
Carmen Dumitru, Carolina Constantin, Cristiana Popp, Mirela Cioplea, Sabina Zurac, Tatiana Vassu, Monica Neagu
Cutaneous melanoma remains a major health issue and still an important challenge for research. Thus omics complex evaluation can provide a more specific molecular classification for this heterogeneous disease. Complex omics analysis based on genomic and proteomic microarrays can identify disease markers that prognosticate disease evolution or can monitor therapies efficacy. Among the technologies that gained momentum in the last years, array based comparative genomic hybridization offered the possibility to analyze chromosomal numerical aberrations within cutaneous melanomas providing important support for molecular classification of melanoma tumors...
June 14, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/28548901/neuroendocrine-differentiation-in-basal-cell-carcinoma
#12
Yoldez Houcine, Ines Chelly, Alia Zehani, Linda Belhaj Kacem, Haifa Azzouz, Wafa Rekik, Hend C, Slim Haouet, Nidhameddine Kchir
Basal cell carcinoma (BCC) is the prototypical basaloid tumor of the skin. It may show various patterns simulating other cutaneous tumors due to its pleomorphism. It may have an unusal pattern of differentiation such as squamous, sebaceous, apocrine, eccrine, pilar, and endocrine differentiation. In order to establish the relative frequency of neuroendocrine differentiation in BCC, we performed a retrospective study of 33 consecutive BCCs using conventional immunohistochemistry with two neuroendocrine antibodies: Chromogranine A and synaptophysine...
May 26, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/28537478/preparation-and-development-of-solid-phase-radioimmunoassay-system-for-determination-of-cortisol
#13
Kh M Sallam
The goal of this article was oriented to prepare stable polystyrene coated tubes for direct radioimmunoassay (RIA) of human cortisol. Coating process was performed using rabbit polyclonal antisera specific for cortisol. The stability study showed that these tubes could be stored for up to one year at 4ºC without any appreciable reduction in their binding. Labeling of cortisol was carried out using chloramin-T method and the (125)I-cortisol tracer was purified using HPLC column. Preparation of polyclonal antibodies (anti-cortisol) was carried out in host rabbit animals against cortisol-21-hemisuccinate:Bovine serum albumin conjugate...
May 24, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/28537469/improvement-of-elisa-procedures-through-simultaneous-addition-of-antigen-and-detection-antibody-and-elimination-of-washing-steps
#14
V Gurevich, P Kotharu, K McCann, J Bertolini
The enzyme-linked immunosorbent assay (ELISA) is extensively used for measurement of proteins utilised for various research and diagnostic purposes in the biological disciplines. However it is a labour intensive and lengthy procedure due to a number of incubation and washing steps required for performing the assay. The ELISA procedure in the current study has been simplified through the simultaneous addition of antigen and detection antibody and elimination of washing steps. This resulted in a decreased time required to perform the procedure and without affecting assay capability...
May 24, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/28521601/significance-of-pregnancy-test-false-negative-results-due-to-elevated-levels-of-%C3%AE-core-fragment-hcg
#15
Sarah Johnson, Saji Eapen, Peter Smith, Graham Warren, Michael Zinaman
Very high levels of β-core fragment human chorionic gonadotrophin (βcf-hCG) are reported to potentially cause false negative results in point-of-care (POC)/over-the-counter (OTC) pregnancy tests. To investigate this further, women's daily early morning urine samples, collected prior to conception and during pregnancy, were analysed for intact, free β-, and βcf-hCG. The proportion of βcf-hCG was found to be related to that of hCG produced and in circulation. Therefore, best practice for accuracy testing of POC/OTC pregnancy tests would be to test devices against clinical samples containing high levels of βcf-hCG as well as standards spiked with biologically relevant ratios...
May 19, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/28475466/an-immunochromatographic-assay-for-rapid-etection-of-salvinorin-a
#16
Madan Kumar Paudel, Osamu Shirota, Seiichi Sakamoto, Satoshi Morimoto, Hiroyuki Tanaka
We developed an immunochromatographic assay (ICA) that enables rapid analysis of salvinorin A (Sal A) in Salvia divinorum within 10 min. The result shows that no Sal A in other samples of Lamiaceae plants was detected, but it could recognize Sal A among other substances in complex samples. The main advantage of the ICA is its high performance in combination with low cost, simplicity, and speed. Our newly developed combined ICA/indirect competitive ELISA(icELISA) system enables analysis of large numbers of samples over short periods of time without cumbersome pretreatments in complex mixtures...
May 5, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/28422565/ascorbic-acid-deficiency-in-patients-with-lichen-planus
#17
Ilinca Nicolae, Cristina Iulia Mitran, Madalina Irina Mitran, Corina Daniela Ene, Mircea Tampa, Simona Roxana Georgescu
OBJECTIVES: Recent studies have highlighted the role of oxidative stress in the pathogenesis of lichen planus (LP). In the present study, the interest of the authors is focused on the investigation of ascorbic acid status in patients with LP and identification of parameters that might influence the level of this vitamin. MATERIAL AND METHOD: We analyzed the level of urinary ascorbic acid (reflectometric method) in 77 patients with LP (cutaneous LP (CLP)-49 cases; oral LP (OLP)-28 cases) and 50 control subjects...
April 19, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/28422564/evaluation-of-two-in-house-immunoenzymatic-tests-to-serodiagnose-subclinical-paratuberculisis-in-a-sheep-flock-in-mexicali-valley-mexico
#18
Elizama Ponce Barraza, Tomás Cárdenas Reyna, Carlos Angulo, José Carlomán Herrera Ramírez, Gilberto V López, Gerardo Enrique Medina-Basulto, Shigetoshi Eda, Sawako Hori-Oshima
Paratuberculosis (PTB) or Johne's disease is a common ruminant infectious disease caused by Mycobacterium avium subsp. paratuberculosis (MAP). In this study, two MAP antigens were compared for their diagnostic utility to detect subclinical PTB in a sheep flock in Mexicali, Mexico. Sheep (n = 31) without clinical signs but positive on a direct fecal-polymerase chain reaction were tested with two preabsorbed in-house enzyme linked immunosorbent assays (ELISAs) using: (1) an ethanol-extracted surface lipid antigen (EVELISA) and (2) a protoplasmic antigen (ELISA-PPA)...
April 19, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/28421869/alk-protein-expression-in-pulmonary-adenocarcinoma-of-tunisian-patients
#19
Amira Toumi Arfaoui, Ahlem Blel, Raoudha Aloui, Ghaya Fatnassi, Jihane Ben Ammar, Nadia Znaidi, Yosra Zidi, Mohammed Sadok Boudaya, Hichem Aouina, Soumaya Rammeh Rommani
BACKGROUND: It is now necessary to determine ALK status in order to use targeted therapy. AIM: herein, we assess immunohistochemical profile of ALK protein in a series of Tunisian patients with pulmonary adenocarcinoma. MATERIALS AND METHODS: ALK protein expression was studied applying the D5F3 antibody with a fully automated Ventana CDx technique on a series of 19 patients. RESULTS: Positive ALK expression was found in one case (5...
April 19, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/28421864/selfie-autoimmunity-boon-or-bane
#20
Haseeb Ahsan
The immune system provides protection to tissues damaged by infectious microrganisms or physical damage. In autoimmune diseases, the immune system recognizes and attacks its own tissues, i.e., self-destruction. Various agents such as genetic factors and environmental triggers are thought to play a major role in the development of autoimmune diseases. A common feature of all autoimmune diseases is the presence of autoantibodies and inflammation, including mononuclear phagocytes, autoreactive T lymphocytes, and autoantibody producing B cells (plasma cells)...
April 19, 2017: Journal of Immunoassay & Immunochemistry
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