journal
MENU ▼
Read by QxMD icon Read
search

EMBO Reports

journal
https://www.readbyqxmd.com/read/27920034/domestication-of-a-housekeeping-transglycosylase-for-assembly-of-a-type-vi-secretion-system
#1
Yoann G Santin, Eric Cascales
The type VI secretion system (T6SS) is an anti-bacterial weapon comprising a contractile tail anchored to the cell envelope by a membrane complex. The TssJ, TssL, and TssM proteins assemble a 1.7-MDa channel complex that spans the cell envelope, including the peptidoglycan layer. The electron microscopy structure of the TssJLM complex revealed that it has a diameter of ~18 nm in the periplasm, which is larger than the size of peptidoglycan pores (~2 nm), hence questioning how the T6SS membrane complex crosses the peptidoglycan layer...
December 5, 2016: EMBO Reports
https://www.readbyqxmd.com/read/27920033/mitochondrial-nuclear-co-evolution-leads-to-hybrid-incompatibility-through-pentatricopeptide-repeat-proteins
#2
Han-Ying Jhuang, Hsin-Yi Lee, Jun-Yi Leu
Mitochondrial-nuclear incompatibility has a major role in reproductive isolation between species. However, the underlying mechanism and driving force of mitochondrial-nuclear incompatibility remain elusive. Here, we report a pentatricopeptide repeat-containing (PPR) protein, Ccm1, and its interacting partner, 15S rRNA, to be involved in hybrid incompatibility between two yeast species, Saccharomyces cerevisiae and Saccharomyces bayanus S. bayanus-Ccm1 has reduced binding affinity for S. cerevisiae-15S rRNA, leading to respiratory defects in hybrid cells...
December 5, 2016: EMBO Reports
https://www.readbyqxmd.com/read/27920032/overselling-hysteria-the-role-of-the-media-and-medical-journals-in-promoting-questionable-risks-a-case-study-of-the-testosterone-controversy
#3
Abdulmaged M Traish, Jay C Vance, Abraham Morgentaler
No abstract text is available yet for this article.
December 5, 2016: EMBO Reports
https://www.readbyqxmd.com/read/27895104/bin1-and-cd2ap-polarise-the-endocytic-generation-of-beta-amyloid
#4
Florent Ubelmann, Tatiana Burrinha, Laura Salavessa, Ricardo Gomes, Cláudio Ferreira, Nuno Moreno, Cláudia Guimas Almeida
The mechanisms driving pathological beta-amyloid (Aβ) generation in late-onset Alzheimer's disease (AD) are unclear. Two late-onset AD risk factors, Bin1 and CD2AP, are regulators of endocytic trafficking, but it is unclear how their endocytic function regulates Aβ generation in neurons. We identify a novel neuron-specific polarisation of Aβ generation controlled by Bin1 and CD2AP We discover that Bin1 and CD2AP control Aβ generation in axonal and dendritic early endosomes, respectively. Both Bin1 loss of function and CD2AP loss of function raise Aβ generation by increasing APP and BACE1 convergence in early endosomes, however via distinct sorting events...
November 28, 2016: EMBO Reports
https://www.readbyqxmd.com/read/27872205/molecular-architecture-of-the-yeast-elongator-complex-reveals-an-unexpected-asymmetric-subunit-arrangement
#5
Dheva T Setiaputra, Derrick Th Cheng, Shan Lu, Jesse M Hansen, Udit Dalwadi, Cindy Hy Lam, Jeffrey L To, Meng-Qiu Dong, Calvin K Yip
Elongator is a ~850 kDa protein complex involved in multiple processes from transcription to tRNA modification. Conserved from yeast to humans, Elongator is assembled from two copies of six unique subunits (Elp1 to Elp6). Despite the wealth of structural data on the individual subunits, the overall architecture and subunit organization of the full Elongator and the molecular mechanisms of how it exerts its multiple activities remain unclear. Using single-particle electron microscopy (EM), we revealed that yeast Elongator adopts a bilobal architecture and an unexpected asymmetric subunit arrangement resulting from the hexameric Elp456 subassembly anchored to one of the two Elp123 lobes that form the structural scaffold...
November 21, 2016: EMBO Reports
https://www.readbyqxmd.com/read/27872204/an-injection-of-confidence-scientists-explore-new-and-old-methods-to-counter-anti-vaccine-propaganda-and-overcome-vaccine-hesitancy-so-as-to-increase-vaccination-rates
#6
https://www.readbyqxmd.com/read/27856536/grwd1-negatively-regulates-p53-via-the-rpl11-mdm2-pathway-and-promotes-tumorigenesis
#7
Kota Kayama, Shinya Watanabe, Takuya Takafuji, Takahiro Tsuji, Kensuke Hironaka, Masaki Matsumoto, Keiichi I Nakayama, Masato Enari, Takashi Kohno, Kouya Shiraishi, Tohru Kiyono, Kazumasa Yoshida, Nozomi Sugimoto, Masatoshi Fujita
The ribosomal protein L11 (RPL11) binds and inhibits the MDM2 ubiquitin ligase, thereby promoting p53 stability. Thus, RPL11 acts as a tumor suppressor. Here, we show that GRWD1 (glutamate-rich WD40 repeat containing 1) physically and functionally interacts with RPL11. GRWD1 is localized to nucleoli and is released into the nucleoplasm upon nucleolar stress. Silencing of GRWD1 increases p53 induction by nucleolar stress, whereas overexpression of GRWD1 reduces p53 induction. Furthermore, GRWD1 overexpression competitively inhibits the RPL11-MDM2 interaction and alleviates RPL11-mediated suppression of MDM2 ubiquitin ligase activity toward p53...
November 17, 2016: EMBO Reports
https://www.readbyqxmd.com/read/27827795/peroxisomal-protein-pex13-functions-in-selective-autophagy
#8
Ming Y Lee, Rhea Sumpter, Zhongju Zou, Shyam Sirasanagandla, Yongjie Wei, Prashant Mishra, Hendrik Rosewich, Denis I Crane, Beth Levine
PEX13 is an integral membrane protein on the peroxisome that regulates peroxisomal matrix protein import during peroxisome biogenesis. Mutations in PEX13 and other peroxin proteins are associated with Zellweger syndrome spectrum (ZSS) disorders, a subtype of peroxisome biogenesis disorder characterized by prominent neurological, hepatic, and renal abnormalities leading to neonatal death. The lack of functional peroxisomes in ZSS patients is widely accepted as the underlying cause of disease; however, our understanding of disease pathogenesis is still incomplete...
November 8, 2016: EMBO Reports
https://www.readbyqxmd.com/read/27799287/cd1d-mediated-activation-of-group-3-innate-lymphoid-cells-drives-il-22-production
#9
Julia Saez de Guinoa, Rebeca Jimeno, Nazanin Farhadi, Peter J Jervis, Liam R Cox, Gurdyal S Besra, Patricia Barral
Innate lymphoid cells (ILCs) are a heterogeneous family of immune cells that play a critical role in a variety of immune processes including host defence against infection, wound healing and tissue repair. Whether these cells are involved in lipid-dependent immunity remains unexplored. Here we show that murine ILCs from a variety of tissues express the lipid-presenting molecule CD1d, with group 3 ILCs (ILC3s) showing the highest level of expression. Within the ILC3 family, natural cytotoxicity triggering receptor (NCR)(-)CCR6(+) cells displayed the highest levels of CD1d...
October 31, 2016: EMBO Reports
https://www.readbyqxmd.com/read/27729390/science-politics-and-policymaking-even-though-expert-knowledge-has-become-indispensible-for-policymaking-providing-scientific-advice-to-governments-is-not-always-easy
#10
https://www.readbyqxmd.com/read/27702987/linear-ubiquitination-by-lubel-has-a-role-in-drosophila-heat-stress-response
#11
Tomoko Asaoka, Jorge Almagro, Christine Ehrhardt, Isabella Tsai, Alexander Schleiffer, Luiza Deszcz, Sini Junttila, Leonie Ringrose, Karl Mechtler, Anoop Kavirayani, Attila Gyenesei, Kay Hofmann, Peter Duchek, Katrin Rittinger, Fumiyo Ikeda
The HOIP ubiquitin E3 ligase generates linear ubiquitin chains by forming a complex with HOIL-1L and SHARPIN in mammals. Here, we provide the first evidence of linear ubiquitination induced by a HOIP orthologue in Drosophila We identify Drosophila CG11321, which we named Linear Ubiquitin E3 ligase (LUBEL), and find that it catalyzes linear ubiquitination in vitro We detect endogenous linear ubiquitin chain-derived peptides by mass spectrometry in Drosophila Schneider 2 cells and adult flies. Furthermore, using CRISPR/Cas9 technology, we establish linear ubiquitination-defective flies by mutating residues essential for the catalytic activity of LUBEL Linear ubiquitination signals accumulate upon heat shock in flies...
October 4, 2016: EMBO Reports
https://www.readbyqxmd.com/read/27702986/gadd45a-is-a-heterochromatin-relaxer-that-enhances-ips-cell-generation
#12
Keshi Chen, Qi Long, Tao Wang, Danyun Zhao, Yanshuang Zhou, Juntao Qi, Yi Wu, Shengbiao Li, Chunlan Chen, Xiaoming Zeng, Jianguo Yang, Zisong Zhou, Weiwen Qin, Xiyin Liu, Yuxing Li, Yingying Li, Xiaofen Huang, Dajiang Qin, Jiekai Chen, Guangjin Pan, Hans R Schöler, Guoliang Xu, Xingguo Liu, Duanqing Pei
Reprogramming of somatic cells to induced pluripotent stem cells rewrites the code of cell fate at the chromatin level. Yet, little is known about this process physically. Here, we describe a fluorescence recovery after photobleaching method to assess the dynamics of heterochromatin/euchromatin and show significant heterochromatin loosening at the initial stage of reprogramming. We identify growth arrest and DNA damage-inducible protein a (Gadd45a) as a chromatin relaxer in mouse embryonic fibroblasts, which also enhances somatic cell reprogramming efficiency...
October 4, 2016: EMBO Reports
https://www.readbyqxmd.com/read/27702985/cancer-specific-mutations-in-p53-induce-the-translation-of-%C3%AE-160p53-promoting-tumorigenesis
#13
Marco M Candeias, Masatoshi Hagiwara, Michiyuki Matsuda
Wild-type p53 functions as a tumour suppressor while mutant p53 possesses oncogenic potential. Until now it remains unclear how a single mutation can transform p53 into a functionally distinct gene harbouring a new set of original cellular roles. Here we show that the most common p53 cancer mutants express a larger number and higher levels of shorter p53 protein isoforms that are translated from the mutated full-length p53 mRNA. Cells expressing mutant p53 exhibit "gain-of-function" cancer phenotypes, such as enhanced cell survival, proliferation, invasion and adhesion, altered mammary tissue architecture and invasive cell structures...
October 4, 2016: EMBO Reports
https://www.readbyqxmd.com/read/27670885/identification-of-ppm1d-as-an-essential-ulk1-phosphatase-for-genotoxic-stress-induced-autophagy
#14
Satoru Torii, Tatsushi Yoshida, Satoko Arakawa, Shinya Honda, Akira Nakanishi, Shigeomi Shimizu
Autophagy is an evolutionary conserved process that degrades subcellular constituents. Unlike starvation-induced autophagy, the molecular mechanism of genotoxic stress-induced autophagy has not yet been fully elucidated. In this study, we analyze the molecular mechanism of genotoxic stress-induced autophagy and identify an essential role of dephosphorylation of the Unc51-like kinase 1 (Ulk1) at Ser(637), which is catalyzed by the protein phosphatase 1D magnesium-dependent delta isoform (PPM1D). We show that after exposure to genotoxic stress, PPM1D interacts with and dephosphorylates Ulk1 at Ser(637) in a p53-dependent manner...
September 26, 2016: EMBO Reports
https://www.readbyqxmd.com/read/27644261/steric-hindrance-of-snare-transmembrane-domain%C3%A2-organization-impairs-the-hemifusion-to-fusion-transition
#15
Massimo D'Agostino, Herre Jelger Risselada, Andreas Mayer
SNAREs fuse membranes in several steps. Trans-SNARE complexes juxtapose membranes, induce hemifused stalk structures, and open the fusion pore. A recent penetration model of fusion proposed that SNAREs force the hydrophilic C-termini of their transmembrane domains through the hydrophobic core of the membrane(s). In contrast, the indentation model suggests that the C-termini open the pore by locally compressing and deforming the stalk. Here we test these models in the context of yeast vacuole fusion. Addition of small hydrophilic tags renders bilayer penetration by the C-termini energetically unlikely...
September 19, 2016: EMBO Reports
https://www.readbyqxmd.com/read/27644260/future-challenges-for-tracking-foodborne-diseases-pulsenet-a-20-year-old-us-surveillance-system-for-foodborne-diseases-is-expanding-both-globally-and-technologically
#16
Efrain M Ribot, Kelley B Hise
No abstract text is available yet for this article.
September 19, 2016: EMBO Reports
https://www.readbyqxmd.com/read/27632992/transcription-factor-anac032-modulates-ja-sa-signalling-in-response-to-pseudomonas-syringae-infection
#17
Annapurna Devi Allu, Yariv Brotman, Gang-Ping Xue, Salma Balazadeh
Responses to pathogens, including host transcriptional reprogramming, require partially antagonistic signalling pathways dependent on the phytohormones salicylic (SA) and jasmonic (JA) acids. However, upstream factors modulating the interplay of these pathways are not well characterized. Here, we identify the transcription factor ANAC032 from Arabidopsis thaliana as one such regulator in response to the bacterial pathogen Pseudomonas syringae pv. tomato DC3000 (Pst). ANAC032 directly represses MYC2 activation upon Pst attack, resulting in blockage of coronatine-mediated stomatal reopening which restricts entry of bacteria into plant tissue...
September 15, 2016: EMBO Reports
https://www.readbyqxmd.com/read/27909074/sulfatase-activities-are-regulated-by-the-interaction-of-the-sulfatase-modifying-factor-1-with-sumf2
#18
Ester Zito, Alessandro Fraldi, Stefano Pepe, Ida Annunziata, Gary Kobinger, Paola Di Natale, Andrea Ballabio, Maria Pia Cosma
No abstract text is available yet for this article.
December 2016: EMBO Reports
https://www.readbyqxmd.com/read/27884820/una-destinatio-viae-diversae-does-exposure-to-the-vaginal-microbiota-confer-health-benefits-to-the-infant-and-does-lack-of-exposure-confer-disease-risk
#19
Kjersti Aagaard, Christopher J Stewart, Derrick Chu
No abstract text is available yet for this article.
December 2016: EMBO Reports
https://www.readbyqxmd.com/read/27872203/molecular-mechanisms-of-asymmetric-divisions-in-mammary-stem-cells
#20
REVIEW
Angela Santoro, Thalia Vlachou, Manuel Carminati, Pier Giuseppe Pelicci, Marina Mapelli
Stem cells have the remarkable ability to undergo proliferative symmetric divisions and self-renewing asymmetric divisions. Balancing of the two modes of division sustains tissue morphogenesis and homeostasis. Asymmetric divisions of Drosophila neuroblasts (NBs) and sensory organ precursor (SOP) cells served as prototypes to learn what we consider now principles of asymmetric mitoses. They also provide initial evidence supporting the notion that aberrant symmetric divisions of stem cells could correlate with malignancy...
December 2016: EMBO Reports
journal
journal
35155
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"