Read by QxMD icon Read

EMBO Reports

Samantha Haller, Adrien Franchet, Abdul Hakkim, Jing Chen, Eliana Drenkard, Shen Yu, Stefanie Schirmeier, Zi Li, Nelson Martins, Frederick M Ausubel, Samuel Liégeois, Dominique Ferrandon
When Drosophila melanogaster feeds on Pseudomonas aeruginosa , some bacteria cross the intestinal barrier and eventually proliferate in the hemocoel. This process is limited by hemocytes through phagocytosis. P. aeruginosa requires the quorum-sensing regulator RhlR to elude the cellular immune response of the fly. RhlI synthesizes the autoinducer signal that activates RhlR. Here, we show that rhlI mutants are unexpectedly more virulent than rhlR mutants, both in fly and in nematode intestinal infection models, suggesting that RhlR has RhlI-independent functions...
March 9, 2018: EMBO Reports
Sandra Stefanovic-Barrett, Anna S Dickson, Stephen P Burr, James C Williamson, Ian T Lobb, Dick Jh van den Boomen, Paul J Lehner, James A Nathan
Misfolded or damaged proteins are typically targeted for destruction by proteasome-mediated degradation, but the mammalian ubiquitin machinery involved is incompletely understood. Here, using forward genetic screens in human cells, we find that the proteasome-mediated degradation of the soluble misfolded reporter, mCherry-CL1, involves two ER-resident E3 ligases, MARCH6 and TRC8. mCherry-CL1 degradation is routed via the ER membrane and dependent on the hydrophobicity of the substrate, with complete stabilisation only observed in double knockout MARCH6/TRC8 cells...
March 8, 2018: EMBO Reports
Warren Pearce, Sarah Hartley, Richard Helliwell, Liz O'Neill
No abstract text is available yet for this article.
March 7, 2018: EMBO Reports
Giovanni Tagliabue
No abstract text is available yet for this article.
March 7, 2018: EMBO Reports
Polyxeni Bozatzi, Kevin S Dingwell, Kevin Zl Wu, Fay Cooper, Timothy D Cummins, Luke D Hutchinson, Janis Vogt, Nicola T Wood, Thomas J Macartney, Joby Varghese, Robert Gourlay, David G Campbell, James C Smith, Gopal P Sapkota
The BMP and Wnt signalling pathways determine axis specification during embryonic development. Our previous work has shown that PAWS1 (also known as FAM83G) interacts with SMAD1 and modulates BMP signalling. Here, surprisingly, we show that overexpression of PAWS1 in Xenopus embryos activates Wnt signalling and causes complete axis duplication. Consistent with these observations in Xenopus , Wnt signalling is diminished in U2OS osteosarcoma cells lacking PAWS1, while BMP signalling is unaffected. We show that PAWS1 interacts and co-localises with the α isoform of casein kinase 1 (CK1), and that PAWS1 mutations incapable of binding CK1 fail both to activate Wnt signalling and to elicit axis duplication in Xenopus embryos...
March 7, 2018: EMBO Reports
Matteo Audano, Silvia Pedretti, Gaia Cermenati, Elisabetta Brioschi, Giuseppe Riccardo Diaferia, Serena Ghisletti, Alessandro Cuomo, Tiziana Bonaldi, Franco Salerno, Marina Mora, Liliana Grigore, Katia Garlaschelli, Andrea Baragetti, Fabrizia Bonacina, Alberico Luigi Catapano, Giuseppe Danilo Norata, Maurizio Crestani, Donatella Caruso, Enrique Saez, Emma De Fabiani, Nico Mitro
Mitochondria are the energy-generating hubs of the cell. In spite of considerable advances, our understanding of the factors that regulate the molecular circuits that govern mitochondrial function remains incomplete. Using a genome-wide functional screen, we identify the poorly characterized protein Zinc finger CCCH-type containing 10 (Zc3h10) as regulator of mitochondrial physiology. We show that Zc3h10 is upregulated during physiological mitochondriogenesis as it occurs during the differentiation of myoblasts into myotubes...
March 5, 2018: EMBO Reports
Xiu-Fei Chen, Meng-Xin Tian, Ren-Qiang Sun, Meng-Li Zhang, Li-Sha Zhou, Lei Jin, Lei-Lei Chen, Wen-Jie Zhou, Kun-Long Duan, Yu-Jia Chen, Chao Gao, Zhou-Li Cheng, Fang Wang, Jin-Ye Zhang, Yi-Ping Sun, Hong-Xiu Yu, Yu-Zheng Zhao, Yi Yang, Wei-Ren Liu, Ying-Hong Shi, Yue Xiong, Kun-Liang Guan, Dan Ye
Peroxisomes account for ~35% of total H2 O2 generation in mammalian tissues. Peroxisomal ACOX1 (acyl-CoA oxidase 1) is the first and rate-limiting enzyme in fatty acid β-oxidation and a major producer of H2 O2 ACOX1 dysfunction is linked to peroxisomal disorders and hepatocarcinogenesis. Here, we show that the deacetylase sirtuin 5 (SIRT5) is present in peroxisomes and that ACOX1 is a physiological substrate of SIRT5. Mechanistically, SIRT5-mediated desuccinylation inhibits ACOX1 activity by suppressing its active dimer formation in both cultured cells and mouse livers...
February 28, 2018: EMBO Reports
Katrin Weigmann
No abstract text is available yet for this article.
February 28, 2018: EMBO Reports
Qi Yi, Qinfu Chen, Cai Liang, Haiyan Yan, Zhenlei Zhang, Xingfeng Xiang, Miao Zhang, Feifei Qi, Linli Zhou, Fangwei Wang
Heterochromatin protein-1 (HP1) is a key component of heterochromatin. Reminiscent of the cohesin complex which mediates sister-chromatid cohesion, most HP1 proteins in mammalian cells are displaced from chromosome arms during mitotic entry, whereas a pool remains at the heterochromatic centromere region. The function of HP1 at mitotic centromeres remains largely elusive. Here, we show that double knockout (DKO) of HP1α and HP1γ causes defective mitosis progression and weakened centromeric cohesion. While mutating the chromoshadow domain (CSD) prevents HP1α from protecting sister-chromatid cohesion, centromeric targeting of HP1α CSD alone is sufficient to rescue the cohesion defects in HP1 DKO cells...
February 28, 2018: EMBO Reports
Ae Lee Jeong, Hye In Ka, Sora Han, Sunyi Lee, Eun-Woo Lee, Su Jung Soh, Hyun Jeong Joo, Buyanravjkh Sumiyasuren, Ji Young Park, Jong-Seok Lim, Jong Hoon Park, Myung Sok Lee, Young Yang
In most mammalian cells, the primary cilium is a microtubule-enriched protrusion of the plasma membrane and acts as a key coordinator of signaling pathways during development and tissue homeostasis. The primary cilium is generated from the basal body, and cancerous inhibitor of protein phosphatase 2A (CIP2A), the overexpression of which stabilizes c-MYC to support the malignant growth of tumor cells, is localized in the centrosome. Here, we show that CIP2A overexpression induces primary cilia disassembly through the activation of Aurora A kinase, and CIP2A depletion increases ciliated cells and cilia length in retinal pigment epithelium (RPE1) cells...
February 28, 2018: EMBO Reports
Jun Hwan Kim, Dongyeob Seo, Sun-Jick Kim, Dong Wook Choi, Jin Seok Park, Jihoon Ha, Jungwon Choi, Ji-Hyung Lee, Su Myung Jung, Kyoung-Wan Seo, Eun-Woo Lee, Youn Sook Lee, Heesun Cheong, Cheol Yong Choi, Seok Hee Park
Autophagy begins with the formation of autophagosomes, a process that depends on the activity of the serine/threonine kinase ULK1 (hATG1). Although earlier studies indicated that ULK1 activity is regulated by dynamic polyubiquitination, the deubiquitinase involved in the regulation of ULK1 remained unknown. In this study, we demonstrate that ubiquitin-specific protease 20 (USP20) acts as a positive regulator of autophagy initiation through stabilizing ULK1. At basal state, USP20 binds to and stabilizes ULK1 by removing the ubiquitin moiety, thereby interfering with the lysosomal degradation of ULK1...
February 27, 2018: EMBO Reports
Shafqat Rasool, Naoto Soya, Luc Truong, Nathalie Croteau, Gergely L Lukacs, Jean-François Trempe
Mutations in PINK1 cause autosomal recessive Parkinson's disease (PD), a neurodegenerative movement disorder. PINK1 is a kinase that acts as a sensor of mitochondrial damage and initiates Parkin-mediated clearance of the damaged organelle. PINK1 phosphorylates Ser65 in both ubiquitin and the ubiquitin-like (Ubl) domain of Parkin, which stimulates its E3 ligase activity. Autophosphorylation of PINK1 is required for Parkin activation, but how this modulates the ubiquitin kinase activity is unclear. Here, we show that autophosphorylation of Tribolium castaneum PINK1 is required for substrate recognition...
February 23, 2018: EMBO Reports
Shai Berlin
No abstract text is available yet for this article.
February 22, 2018: EMBO Reports
Baile Wang, Ang Li, Xiaomu Li, Philip Wl Ho, Donghai Wu, Xiaoqi Wang, Zhuohao Liu, Kelvin Kl Wu, Sonata Sy Yau, Aimin Xu, Kenneth Ky Cheng
Activation of brown adipose tissue (BAT) and beige fat by cold increases energy expenditure. Although their activation is known to be differentially regulated in part by hypothalamus, the underlying neural pathways and populations remain poorly characterized. Here, we show that activation of rat-insulin-promoter-Cre (RIP-Cre) neurons in ventromedial hypothalamus (VMH) preferentially promotes recruitment of beige fat via a selective control of sympathetic nervous system (SNS) outflow to subcutaneous white adipose tissue (sWAT), but has no effect on BAT Genetic ablation of APPL2 in RIP-Cre neurons diminishes beiging in sWAT without affecting BAT, leading to cold intolerance and obesity in mice...
February 21, 2018: EMBO Reports
Jan B Heidelberger, Andrea Voigt, Marina E Borisova, Giuseppe Petrosino, Stefanie Ruf, Sebastian A Wagner, Petra Beli
Valosin-containing protein (VCP) is an evolutionarily conserved ubiquitin-dependent ATPase that mediates the degradation of proteins through the ubiquitin-proteasome pathway. Despite the central role of VCP in the regulation of protein homeostasis, identity and nature of its cellular substrates remain poorly defined. Here, we combined chemical inhibition of VCP and quantitative ubiquitin remnant profiling to assess the effect of VCP inhibition on the ubiquitin-modified proteome and to probe the substrate spectrum of VCP in human cells...
February 21, 2018: EMBO Reports
Thomas O'Loughlin, Thomas A Masters, Folma Buss
The intracellular functions of myosin motors requires a number of adaptor molecules, which control cargo attachment, but also fine-tune motor activity in time and space. These motor-adaptor-cargo interactions are often weak, transient or highly regulated. To overcome these problems, we use a proximity labelling-based proteomics strategy to map the interactome of the unique minus end-directed actin motor MYO6. Detailed biochemical and functional analysis identified several distinct MYO6-adaptor modules including two complexes containing RhoGEFs: the LIFT (LARG-Induced F-actin for Tethering) complex that controls endosome positioning and motility through RHO-driven actin polymerisation; and the DISP (DOCK7-Induced Septin disPlacement) complex, a novel regulator of the septin cytoskeleton...
February 21, 2018: EMBO Reports
Arthur L Caplan
No abstract text is available yet for this article.
February 21, 2018: EMBO Reports
Alfred W Bronkhorst, René F Ketting
No abstract text is available yet for this article.
February 19, 2018: EMBO Reports
Manuel Haschka, Gerlinde Karbon, Luca L Fava, Andreas Villunger
Interfering with mitosis for cancer treatment is an old concept that has proven highly successful in the clinics. Microtubule poisons are used to treat patients with different types of blood or solid cancer since more than 20 years, but how these drugs achieve clinical response is still unclear. Arresting cells in mitosis can promote their demise, at least in a petri dish. Yet, at the molecular level, this type of cell death is poorly defined and cancer cells often find ways to escape. The signaling pathways activated can lead to mitotic slippage, cell death, or senescence...
February 19, 2018: EMBO Reports
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"