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Current Pharmaceutical Biotechnology

Yu Deng, Zhigang Huang, Wenbing Wang, Yinghuai Chen, Zhongning Guo, Ying Chen
BACKGROUND: Aiming to improve the laser induced forward transfer (LIFT) cell isolation process, a polydimethylsiloxane (PDMS) layer with micro-hole arrays was employed to improve the cell separation precision, and a microchip with heater was developed to maintain the working area at 100% humidity and 37oC with the purpose to preserve the viability of the isolated cells. METHODS: A series of experiments were conducted to verify the contributions of the optimization to LIFT cell isolation process as well as to study the effect of laser pulse energy, laser spot size and the titanium thickness on cell isolation...
March 14, 2018: Current Pharmaceutical Biotechnology
Yat Hei Leung, Francois Belanger, Jennifer Lu, Jacques Turgeon, Veronique Michaud
BACKGROUND: Drug-induced myopathy is a serious side effect that often requires removal of a medication from a drug regimen. For most drugs, the underlying mechanism of drug-induced myopathy remains unclear. Monocarboxylate transporters (MCTs) mediate L-lactic acid transport, and inhibition of MCTs may potentially lead to perturbation of L-lactic acid accumulation and muscular disorders. Therefore, we hypothesized that L-lactic acid transport may be involved in the development of drug-induced myopathy...
March 7, 2018: Current Pharmaceutical Biotechnology
Rao Mukkavilli, Gajanan Jadhav, Subrahmanyam Vangala
BACKGROUND: Drug transporters function as gatekeepers and modulate drug access into the body and various tissues. Thus a thorough and precise understanding of transporter liability for compound uptake and efflux is critical during drug development. METHODS: In the present study, we assessed the apparent permeability (Papp) and compared the efflux ratio of various compounds in stably transfected Madin-Darby Canine Kidney (MDCKII) cells overexpressing human P-gp (MDCKII-P-gp), human BCRP (MDCKII-BCRP), wild type (MDCKII-WT), and Caco-2 cell monolayers...
March 7, 2018: Current Pharmaceutical Biotechnology
Rajesh Dabur, Amey Shirolkar, Vijender Mishra, Baljeet Singh Yadav
BACKGROUND: Excessive alcohol consumption damages the intestine and liver cells directly as well as through unbalancing the gut microbiota. OBJECTIVE: The current study was undertaken to correlate the alcohol consumption and change in urinary metabolites profile linked with gut microbiota. METHOD: Non-alcoholic (control) healthy (n=22) and moderate alcoholic (n=26) males with an average age of 39.3±1.83 years subjected to alcohol use disorders identification test (AUDIT) were considered for study...
March 7, 2018: Current Pharmaceutical Biotechnology
Saad Aboualkasem, Debbie S Retnoningrum, Aldila Refiani, Catur Riani
BACKGROUND: Expression vector is an important component in the production of therapeutic recombinant proteins. Most of commercialized expression vectors apply antibiotic-based selection system. Meanwhile, World Health Organization highly recommends for alternative system due to its potentials to cause spreading of resistance gene and hypersensitivity to some people. METHODS: In current work, we developed an expression system for Escherichia coli using toxin-antidote system in two separated plasmids...
February 26, 2018: Current Pharmaceutical Biotechnology
Xuehui Liu, Rongrong Zhou, Bingbing Shen, Lin Chen, Qingyijun Zhou, Dan Wan, Shuihan Zhang
BACKGROUND: Lonicera macranthoides is a Chinese herb that contains a large number of bioactive spanions possessing important pharmacological activities, such as anti-tumour activity. However, detailed information about their anti-tumor activity and bioactive compounds is limited. METHODS: In order to evaluate the scientific basis, the method of high-speed counter-current chromatography (HSCCC) combined with high performance liquid chromatography mass spectrometry (HPLC-ESI-QTOF/MS) has been developed to separate, purify and analyze saponins from Lonicera macranthoides...
February 26, 2018: Current Pharmaceutical Biotechnology
Petra Lovecka, Jan Lipov, Kamila Thumova, Anna Macurkova
BACKGROUND: The aim of this work was to compare water and organic extracts, infusions and tinctures from flowers and leaves of Calendula officinalis in terms of their biological activity and composition. The purpose of work was investigation whether the leaves and stems are really the waste or they contain interesting substances which could be utilized. Antimicrobial, antifungal, antioxidant and anti-inflammatory activities were studied. Then the ability to inhibit collagenase was studied as well...
February 26, 2018: Current Pharmaceutical Biotechnology
Chi Zhang, Zhichun Gu, Long Shen, Xianyan Liu, Houwen Lin
BACKGROUND: Alzheimer's disease (AD) is one of most serious threat for human beings, however, the treatment was hindered by blood brain barrier and poor intra-brain cell selectivity. METHODS: In this study, we developed a novel dual targeting drug delivery system by modification NL4 peptide and apolipoprotein A-I (ApoA-I) onto dendrimer particles that may efficiently deliver siRNA into neuron cells to down-regulate BACE1 and inhibit Aβ formation. The constructed ANNP/siRNA was approximately 79...
February 26, 2018: Current Pharmaceutical Biotechnology
Antonio Di Sario, Paola Sassaroli, Luigi Daretti, Giulia Annulli, Laura Schiada, Giorgia Falcioni, Emanuele Bendia, Simone Antuono, Antonio Benedetti
BACKGROUND: Up to 80% of Crohn`s disease (CD) patients require at least one surgical intervention in their lifetime and up to 70% of these patients develop postoperative endoscopic recurrence within 1 year. The most important predictors of early postoperative recurrence are represented by smoking, prior intestinal surgery, penetrating disease and perianal location. Genetic factors, gut microbiota structure and immunological alterations may be involved in the pathogenesis of postoperative recurrence of CD, although their specific roles have to be determined yet...
February 16, 2018: Current Pharmaceutical Biotechnology
Tahir Rasheed, Muhammad Bilal, Chuanlong Li, Hafiz M N Iqbal
BACKGROUND: In the present study, the potential of methanolic leaf extract of Taraxacum officinale plant as a function of bio-inspired green synthesis for the fabrication of silver nanoparticles (AgNPs) has been explored. METHODS: The bio-reduction of aqueous silver nitrate (AgNO3) solution was confirmed by visually detecting the color change from pale yellow to blackish-brown. Maximum absorbance was observed at 420 nm due to the presence of characteristic surface Plasmon resonance of nano silver by UV-visible spectroscopy...
February 14, 2018: Current Pharmaceutical Biotechnology
Abdul Sami Shaikh, Ruichen Guo
BACKGROUND: Phenytoin has very challenging pharmacokinetic properties. To prevent its toxicity and ensure efficacy, continuous therapeutic monitoring is required. It is hard to get a simple, accurate, rapid, easily available, economical and highly sensitive assay in one method for therapeutic monitoring of phenytoin. OBJECTIVE: The present study is directed towards establishing and validating a more simple, rapid, an accurate, highly sensitive, novel and environment friendly liquid chromatography/mass spectrometry (LC/MS) method for offering rapid and reliable TDM results of phenytoin in epileptic patients to physicians and clinicians for making immediate and rational decision...
February 9, 2018: Current Pharmaceutical Biotechnology
Danciu Corina, Bojin Florina, Pinzaru Iulia, Dehelean Cristina, Ambrus Rita, Popescu Alexandra, Paunescu Virgil, Monica Hancianu, Minda Daliana, Soica Codruta
BACKGROUND: Recent studies have shown that rutin present a heightened interest due to the plethora of biological activities. The major drawback for this phytocompound is the poor water solubility, parameter that limits its bioavailability. The study aimed to prepare and assess the inclusion complexes of rutin with β-cyclodextrin (BCD) and hydroxypropyl-β-cyclodextrin (HPBCD) followed by the evaluation of the antioxidant, antiproliferative and pro-apoptotic activity against B164A5 melanoma cell line...
February 9, 2018: Current Pharmaceutical Biotechnology
Bahareh Vakili, Navid Nezafat, Manica Negahdaripour, Maryam Yari, Bijan Zare, Younes Ghasemi
BACKGROUND: One of the most important causes of death in the modern lifestyle is acute ischemic stroke, which is related to thrombosis in the blood vessels. Staphylokinase (SAK), a fibrinolytic agent, which is produced mainly by Staphylococcus aureus, is an indirect activator of plasminogen and belongs to the third generation of fibrinolytic enzymes. METHODS: Considering the very low level of production and immunogenicity concerns of natural SAK produced by Staphylococcus aureus, attempts have been made to produce recombinant SAKs with high production levels, more fibrinolytic activities and low immunogenicity...
February 9, 2018: Current Pharmaceutical Biotechnology
Fatemeh Safari, Safar Farajnia, Younes Ghasemi, Nosratollah Zarghami
BACKGROUND: RNA-guided endonuclease as a versatile genome editing technology opened new windows in various fields of biology. The simplicity of this revolutionary technique provides a promising future for its application in a broad range of approaches from functional annotation of genes to diseases, to genetic manipulation and gene therapy. Besides the site-specific activity of Cas9 endonuclease, the unintended cleavage known as off-target effect is still a major challenge for this genome editing technique...
February 9, 2018: Current Pharmaceutical Biotechnology
A Campanati, E Molinelli, G Ganzetti, V Brisigotti, A Offidani
BACKGROUND: Psoriasis is a chronic immune-mediated inflammatory disorder, with an estimated global prevalence of 2-3%. Psoriasis is associated with an impaired health-related quality of life and a substantial economic burden. Biologics, which target the pathways involved in the pathogenesis of psoriasis, represent an established therapeutic approach for moderate-to-severe plaque psoriasis, with remarkable efficacy and safety profile extensively examined and monitored. METHODS: Biological therapies currently available can be divided into three main categories: the TNFα antagonists (infliximab, adalimumab, etanercept, golimumab, certolizumab pegol), the interleukin (IL)-12/23 monoclonal antibody (ustekinumab), and IL-17 inhibitor (secukinumab, ixekizumab)...
February 9, 2018: Current Pharmaceutical Biotechnology
Szymon Chowanski, Grzegorz Rosinski
In mammals, the cholinergic nervous system plays a crucial role in neuronal regulation of physiological processes. It acts on cells by two types of receptors - nicotinic and muscarinic receptors. Both signal transmission pathways also operate in the central and peripheral cholinergic nervous system of insects. METHODS: In our pharmacological experiments, we studied the effects of two muscarinic agonists (carbachol, pilocarpine) and two muscarinic antagonists (atropine, scopolamine) on the muscle contractile activity of visceral organs in the beetle, Tenebrio molitor...
January 24, 2018: Current Pharmaceutical Biotechnology
Mahboubeh Zarei, Navid Nezafat, Mohammad Hossein Morowvat, Ali Dehshahri, Mohammad Bagher Ghoshoon, Aydin Berenjian, Younes Ghasemi, Junhua Peng
BACKGROUND: Microgeographic studies of molecular markers could reveal the nature and dynamics of genetic diversity and the evolutionary driving forces shaping evolution. METHODS: The microclimatic genetic divergence of wild emmer wheat associated with solar radiation was investigated, in the present study, using multiple types of molecular markers including allozyme, amplified polymorphic DNA (RAPD), simple sequence repeat (SSR), and single nucleotide polymorphisms (SNP)...
January 24, 2018: Current Pharmaceutical Biotechnology
Mohamed Z Gad, Noura Ayman Ahmed Abdelmonem, Nancy Osama Elsayed Turky
BACKGROUND: Thrombospondin (TSP) 1 and 4 are extracellular matrix glycoproteins that mediate cell proliferation, platelet aggregation and inflammatory response. Conflicting data addressed the possible contribution of TSP-1 and TSP-4 gene polymorphisms to acute myocardial infarction (AMI). OBJECTIVE: Our study aimed to examine the association of TSP-1 (N700S) and TSP-4 (A387P) genetic variants with the incidence of AMI in Egyptians. It also correlated TSP-1 variants to TSP-1 and TNF-α serum concentrations while TSP-4 variants to IL-8 concentration identifying TSPs' contribution to vascular inflammation...
January 15, 2018: Current Pharmaceutical Biotechnology
Mahboubeh Zarei, Navid Nezafat, Mohammad Hossein Morowvat, Ali Dehshahri, Mohammad Bagher Ghoshoon, Aydin Berenjian, Younes Ghasemi
OBJECTIVE: Optimization of the medium for recombinant arginine deiminase production in E. coli was performed using response surface methodology. This is the first study of optimization of recombinant arginine deiminase production in E. coli by the use of response surface methodology. METHODS: A Mycoplasm arginine deiminase gene was computationally optimized and inserted into pET-3a (+) expression vector. The synthetic pET3a-arginine deiminase construct was cloned and overexpressed in E...
January 15, 2018: Current Pharmaceutical Biotechnology
Jelena Vukojevic, Mirjana Stajic, Jasmina Cilerdzic
BACKGROUND: Microfungi are causal agents of numerous diseases and disorders of agricultural plants, farm mushrooms and animals as well as human, which results are serious global reduction of the food amount, decrease of life quality, the severe life-threatening diseases and enormous economic losses. METHODS: In spite of organism innate ability to combat against pathogens, in invasions of some pathogens, support of additional antimycotic agents to defence system is required...
January 15, 2018: Current Pharmaceutical Biotechnology
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