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Current Pharmaceutical Biotechnology

M Marenco, L Lodola, M G Persico, V Frangipane, A Facoetti, C Aprile, M Hodolic
Background Neurotensin receptors are overexpressed in several cancer types including pancreatic ductal adenocarcinoma. Three NTR subtypes have been cloned: NTR-1, NTR-2 and NTR-3. The most expressed NTR-1 is not present in normal pancreatic tissue and has a low expression in chronic pancreatitis. Objective Objective of this study was to test in vitro affinity of the new 68Ga labelled neurotensin analogue DOTA-NT-20.3 (fragment 6-13, Ac-Lys(DOTA)-Pro-Arg(N-CH3)-Arg-Pro-Tyr-Tle-Leu) on the human pancreatic ductal adenocarcinoma cell line AsPC-1...
August 29, 2018: Current Pharmaceutical Biotechnology
Dinali Ranmadugala, Megan Grainger, Merilyn Manley-Harris, Aydin Berenjian
BACKGROUND: An efficient and accurate HPLC method was developed for the determination of menaquinone-7 (MK-7) in microbial fermentation using 2-propanol and n-hexane as extraction solvents as well as the eluent. METHODS: Extraction was carried out with 2-propanol and n-hexane (2:1, v/v) after enzymatic hydrolysis with 1% (w/v) lipase and ethanol water treatment prior to quantification in order to remove interfering lipids and denatured proteins. Chromatographic separation of MK-7 was accomplished isocratically on a C 18 Gemini column using a mobile phase mixture of 2- propanol: n-hexane (2:1, v/v) with a flow rate of 0...
August 27, 2018: Current Pharmaceutical Biotechnology
Fazlurrahman Khan, Mohammad Mansoob Khan, Young-Mog Kim
BACKGROUND: A novel strategy has been adapted to combat the threat caused by biofilm forming-pathogenic bacteria in our environments. It involves synthesis of antibiofilm compounds biologically (metabolites from animals, microbes and plants) and chemically. As a result of extensive research significant number of antimicrobial compounds and biofilm inhibitors have been isolated and characterized from different biological and chemical sources. However, lots of limitations such as poor delivery, water-insolubility, stability, expulsion by efflux pumps, and development of acquired resistance due to long-term exposure have been associated with these compounds...
August 27, 2018: Current Pharmaceutical Biotechnology
Jiban Barman, Ritesh Kumar, Gundappa Saha, Kartikeya Tiwari, Vikash Kumar Dubey
Apoptosis, a form of programmed cell death, plays a very crucial role in various physiological processes for maintaining the homeostasis. This process is characterized by membrane blebbing, nuclear condensation, DNA fragmentation and cell shrinkage. Any defect in this highly regulated process eventually leads to extended cell survival and could result in neoplastic cell expansion followed by genetic instability. The apoptotic machinery is mainly processed and regulated by various caspases, a family of cysteine proteases...
August 20, 2018: Current Pharmaceutical Biotechnology
Amira Zairi, Sahar Nouir, Naceur M'hamdi, Marwa Bennani, Ines Bergaoui, Ali Mtiraoui, Maher Chaouachi, Mounir Trabelsi
BACKGROUND: The plant species Rosmarinus officinalis (RO), Thymus algeriensis (TA) and Thymus capitatus (TC) are widely used in traditional medicine in Tunisia. Their bioactivities have been reported before and particularly referred to their essential oils. The main objective of this work was to assess the phytochemical composition, the antioxidant activity, the antibacterial, antifungal, and cytotoxic potential of these 3 plants. METHODS: The High Performance Liquid Chromatography (HPLC), chemical tests and spectrophotometric methods were used for screening, quantification of phytochemicals and for antioxidant activities...
August 17, 2018: Current Pharmaceutical Biotechnology
Abdul Sami Shaikh, Xi Guo, Yi Li, Lili Cao, Xuewu Liu, Pingli Li, Rui Zhang, Ruichen Guo
BACKGROUND: The impact of antiepileptics on serum vitamin levels is controversial and uncertain. With no clear conclusions on the impact of antiepileptics on serum levels of vitamins, there is a need for further clinical studies in order to ascertain the impact of old and newer antiepileptic drugs on serum levels of vitamins in epileptic patients, thus accomplishing a suitable usage of vitamins supplementation. OBJECTIVE: The intention of the present research is to confirm the hypothesis of whether or not vitamin levels are altered with antiepileptic drugs...
August 15, 2018: Current Pharmaceutical Biotechnology
Antonio Pecoraro, Stephen Jolles, Ludovica Crescenzi, Gilda Varricchi, Giancarlo Marone, Marcella Savoia, Arturo Genovese, Giuseppe Spadaro
Morbidity and mortality of primary and secondary antibody deficiencies (AD) are frequently associated with diagnostic delays. These could be avoided by a combination of factors including a widespread and effective development in screening tests. Calculated globulin (CG), derived from the difference between serum total protein and albumin levels, reflects immunoglobulin serum levels and has shown to have a predictive value in the early diagnosis of antibody deficiencies. This study investigated the possibility to use low levels of CG to detect antibody deficiency in an Italian University Hospital...
August 8, 2018: Current Pharmaceutical Biotechnology
Teresa Pasqua, Pasquale Pagliaro, Carmine Rocca, Tommaso Angelone, Claudia Penna
Background Hypertension is a multifactorial and chronic cardiovascular condition whose complications are responsible for worldwide morbidity and mortality. An increasing body of experimental data, recognize low-grade inflammation as a basic process in hypertension onset and development since there is a strong contribution of both the innate and the adaptive immune system according to the so-called Danger-Model. In this contest, NLRP3 inflammasome represents a key signaling platform as demonstrated by its implication in several hypertension-associated conditions, such as vascular smooth muscle remodeling and proliferation...
August 8, 2018: Current Pharmaceutical Biotechnology
Francesco Moccia, Roberto Berra-Romani, Vittorio Rosti
Endothelial progenitor cells (EPCs) are mobilized in peripheral blood to rescue blood perfusion in ischemic tissues. Several approaches were, therefore, designed to inject autologous EPCs and induce therapeutic angiogenesis in patients affected by cardiovascular disorders. Endothelial colony forming cells (ECFCs) represent the only truly endothelial precursor and are regarded as the most suitable substrate for cell based therapy of ischemic diseases. Intracellular Ca2+ signalling drives ECFC proliferation, migration, homing and neovessel formation...
August 8, 2018: Current Pharmaceutical Biotechnology
Vidyanath Jha, Rachana Shalini, Anubha Kumari, Prajna Jha, Nand Kishore Sah
BACKGROUND: Euryale ferox Salisb. (Makhana) is a rooted, macro-hydrophyte grown as a crop in north Bihar wetlands, India. Makhana refers to yummy pops prepared through mechanical smacking of the baked seeds of E. ferox. New techniques have been evolved to produce pops from seeds at a brisk pace. OBJECTIVE: It is specially associated with marital rituals possibly in view of its spermatogenic properties. It has a high amino acid index but a low glycemic value. The plant carries medicinal values against cardiovascular and diabetes-related diseases...
August 8, 2018: Current Pharmaceutical Biotechnology
Sen-Mao Li, Jia Hu
BACKGROUND: Small double-strand RNAs (dsRNAs) molecules are able to activate endogenous genes via an RNA-based promoter-targeting mechanism. Like RNA interference (RNAi), RNA activation (RNAa) is an evolutionarily conserved mechanism that is present in diverse eukaryotic organisms ranging from yeast to humans. METHODS: The small activating RNAs (saRNAs) that are involved in RNAa have been successively used to activate gene expression in cultured cells. Thus, this emergent technique might allow us to develop biotechnological and therapeutic applications without the need to synthesize hazardous construct systems that harbor exogenous DNA sequences...
August 2, 2018: Current Pharmaceutical Biotechnology
Spaska Angelova Stanilova, Julieta Borisova Gerenova, Lyuba Dineva Miteva, Irena Manolova
BACKGROUND: TGF-β1 gene (TGFB1) is one of the target genes involved in genetic predisposition to autoimmune diseases, particularly Hashimoto's thyroiditis (HT). OBJECTIVE: In the present study, we attempted to investigate whether -509C/T SNP (rs1800469) in the promoter of TGFB1 is associated with the genetic susceptibility and clinical characteristics of Bulgarian patients with HT. We also analyzed serum TGF-β1 levels in different stages of the disease and its association with the -509C/T polymorphism in the TGFB1 promoter...
August 2, 2018: Current Pharmaceutical Biotechnology
Kashan Khan, Saleem Javed
BACKGROUND: Nanoparticles (NPs) or nanomaterials being used widely in various fields have occupied prime importance in biomedical sciences, owing to their unique size dependent properties which make them superior to bulk materials. One of the major applications of NPs in biomedical field is their therapeutic application including antimicrobial activity. OBJECTIVE: It is already well known that certain NPs such as silver, zinc oxide, copper, iron etc. bears significant antimicrobial activity as they release metal ions which subsequently generate reactive oxygen species (ROS), consequently demonstrating potential to be a better alternative to antibiotics and other antimicrobial agents...
July 31, 2018: Current Pharmaceutical Biotechnology
Hawra Al-Ghafli, Sahal Al-Hajoj
BACKGROUND: Despite exerted efforts to control and treat Mycobacterium tuberculosis (MTB) strains, Tuberculosis (TB) remains a public health menace. The emergence of complex drug-resistant profiles, such as multi-drug resistant and extensively drug-resistant MTB strains, emphasizes the need for early diagnosis of resistant cases, shorter treatment options, and effective medical interventions. OBJECTIVE: Solutions for better clinical management of drug resistant cases are either pathogen-centered (novel chemotherapy agents) or host-directed approaches (modulating host immune response to prevent MTB invasion and pathogenesis)...
July 31, 2018: Current Pharmaceutical Biotechnology
Marijana Tucak, Daniela Horvat, Tihomir Cupic, Goran Krizmanic, Vesna Tomas, Marija Ravlic, Svetislav Popovic
BACKGROUND: Alfalfa and red clover are the most widespread and most important perennial legumes, primarily used as a high quality feed for livestock. Both alfalfa and red clover, as well as some other plant species from Fabaceae family, are a rich natural source of phytoestrogens, nonsteroidal compounds with estrogenic activity whose beneficial effects in the prevention and treatment of many diseases are demonstrated in numerous scientific studies. OBJECTIVES: Nowadays, various herbal preparations are present on the world market and their use is constantly increasing, as well as the growing demands of consumers for environmentally sound and health-safe production of plant species used as sources of active substances...
July 30, 2018: Current Pharmaceutical Biotechnology
Malgorzata Miastkowska, Monika Konieczna, Elwira Lason, Malgorzata Tabaszewska, Elzbieta Sikora, Jan Ogonowski
BACKGROUND: Skin cancers are the most common malignancy of humans, and the number of cases has increased dramatically in the past few decades. Therefore, it is very important to carry out studies concerning new and safer anticancer natural agents (e.g. perillyl alcohol) and modern drug delivery systems, such as nanoformulations, which increase their bioavailability. OBJECTIVES: The aim of this work was to obtain different kinds of topical vehicles formulation and compare their efficiency in the release of perillyl alcohol...
July 30, 2018: Current Pharmaceutical Biotechnology
Beniamin Grabarek, Dominika Wcislo-Dziadecka, Joanna Gola, Celina Kruszniewska-Rajs, Ligia Brzezinska-Wcislo, Nikola Zmarzly, Urszula Mazurek
OBJECTIVE: The aim of this study was to evaluate the influence of adalimumab on expression pattern of genes associated with JAK/STAT signaling pathway in Normal Human Dermal Fibroblast (NHDF) cells stimulated with 8 µg/ml of adalimumab and the identification of miRNAs regulating these genes' expression. METHODS: NHDFs were cultured with or without the presence of adalimumab for 2, 8, and 24h. The microarray technology was used to determine expression profile of mRNAs and miRNAs...
July 29, 2018: Current Pharmaceutical Biotechnology
Wioletta Siemiradzka, Barbara Dolinska, Florian Ryszka
BACKGROUND: There are many calcium supplements available on the market, especially those containing calcium in the form of carbonate, which unfortunately is not absorbed by the body to a sufficient degree. METHODS: Therefore, an attempt was made to prepare new sources of calcium, consuming the chicken eggshells as natural raw materials, which were used in preparation of tablets containing calcium carbonate and calcium citrate as well as tablets with calcium carbonate and calcium bisglycinate...
July 22, 2018: Current Pharmaceutical Biotechnology
Brunelli da Ros Peruch, Brenna Lepaus Monteiro, Rafaela Aires, Cristina Martins E Silva, Marco Cesar Cunegundes Guimaraes, Elisardo Corral Vasquez, Breno Valentim Nogueira
BACKGROUND: Stroke is a major cause of severe and long-term disability in adult individuals. Treatment of this disease is limited by the narrow therapeutic window in which intervention is crucial. An alternative therapy for stroke could be cellular growth factors, which participate in several pathways that mediate neuronal cell death. METHODS: We evaluated the neuroprotective ability of different doses of granulocyte colony-stimulating factor (G-CSF; 5, 50, and 100 μg/kg/day) in the mouse model of global cerebral ischemia induced by bilateral occlusion of the common carotid arteries for 80 minutes...
July 17, 2018: Current Pharmaceutical Biotechnology
Roghayyeh Baghban, Safar Farajnia, Younes Ghasemi, Mojtaba Mortazavi, Nosratollah Zarghami, Naser Samadi
BACKGROUND: Although Pichia pastoris is an outstanding host among conventional expression systems for production of recombinant proteins, a new interest have been emerged to this system due to the inherent advantages and new developments in this expression host. The potential for secretory and soluble expression of heterologous glycoproteins in P. pastoris proposed this system as a candidate for the production of complex eukaryotic proteins. METHODS: Several new developments have been occurred in different areas related to P...
July 17, 2018: Current Pharmaceutical Biotechnology
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