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Current Pharmaceutical Biotechnology

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https://www.readbyqxmd.com/read/27855600/optimization-on-fc-for-improvement-of-stability-and-aggregation-resistance
#1
Xiaobo Chen, Fang Zeng, Tao Huang, Liang Cheng, Huan Liu, Rui Gong
Fc-based therapeutics including therapeutic full-size monoclonal antibodies (mAbs) and Fc-fusion proteins represent fastest-growing market in biopharmaceutical industrial. However, one major challenge during development of Fc-based therapeutics is how to maintain their efficacy in clinic use. Many factors may lead to failure in final marketing. For example, the stability and aggregation resistance might not be high enough for bearing the disadvantages during fermentation, purification, formulation, storage, shipment and other steps in manufacture and sale...
November 17, 2016: Current Pharmaceutical Biotechnology
https://www.readbyqxmd.com/read/27855599/a-highly-polar-phytocomplex-involving-rutin-is-responsible-for-the-neuromuscular-facilitation-caused-by-casearia-sylvestris-gua%C3%A3-atonga
#2
Yoshida Eh, Tribuiani N, Foramiglio Al, Foramiglio Ca, Tavares Rvs, Bonomini Tj, Bueno Pcp, Cavalheiro Aj, Hyslop S, Puebla P, San Feliciano A, Oshima-Franco Y
BACKGROUND: Of the various biological activities ascribed to extracts from Casearia sylvestris (guaçatonga), its facilitatory activity, i.e., ability to increase skeletal muscle contractile amplitude, has promising therapeutic applications. In this work, we investigated the components responsible for the previously described neurofacilitation caused by C. sylvestris leaves. METHODS: The methanolic fraction of C. sylvestris leaves was initially fractionated by column chromatography and partitioned in a MeOH:H2O gradient...
November 17, 2016: Current Pharmaceutical Biotechnology
https://www.readbyqxmd.com/read/27903235/erythropoietin-enhances-the-cytotoxic-effect-of-hydrogen-peroxide-on-colon-cancer-cells
#3
Tankiewicz-Kwedlo Anna, Hermanowicz Justyna Magdalena, Surażynski Arkadiusz, Kwedlo Wojciech, Rożkiewicz Dariusz, Pawlak Krystyna, Domaniewski Tomasz, Pawlak Dariusz
BACKGROUND: Cancer patients treated with alkylating agents and radiotherapy are exposed to high level of reactive oxygen species (ROS) in tissues. ROS can involve superoxide free radicals, peroxynitrite, singlet oxygen, nitric oxide and hydrogen peroxide. It is well documented that increased exposure to oxygen through a high metabolic rate could lead to a shortened life span. Ionizing radiation, use of drugs and the development of cancer can lead to cancer-induced anemia. Recombinant human erythropoietin (Epo) supplementation is one of the methods for treating anemia...
November 15, 2016: Current Pharmaceutical Biotechnology
https://www.readbyqxmd.com/read/27842482/antibiotic-release-from-calcium-phosphate-materials-in-oral-and-maxillofacial-surgery-molecular-cellular-and-pharmaceutical-aspects
#4
Angel Manchon, Juan Carlos Prados-Frutos, Carmen Rueda Rodriguez, Carmen Salinas Goodier, Mohammad Hadman Alkhraisat, Rosa Rojo, Arantza Rodriguez Gonzalez, Ana Berlanga, Enrique Lopez Cabarcos
Calcium phosphate materials (CPM) are widely used in dentistry and maxillofacial surgery. The presence of microbial biofilms and external infections are responsible for the failure of many procedures of dental implants and bone grafts. In an attempt to reduce the percentage of these infectious processes antibiotics have been associated with CPM improving certain conditions. For instance antibiotics administered orally or intravenously have less effect and the blood flow in relation to this is poor near implants and grafts...
November 14, 2016: Current Pharmaceutical Biotechnology
https://www.readbyqxmd.com/read/27842481/igg-fc-fragment-as-a-scaffold-for-development-of-targeted-therapeutics
#5
Gordana Wozniak-Knopp, Gerhard Stadlmayr, Florian Rueker
Monoclonal antibodies and Fc fusion proteins have been successful therapeutics in the field of cancer and immune disease. As their pharmacological activity is dependent on the Fc fragment governing their effector functions and long in vivo half-life, the extensive engineering of the Fc for altered binding of its natural ligands that enable these properties has delivered molecules optimized for their therapeutic effect. Recently, the IgG1 Fc region itself and its subunits monomeric Fc fragment, CH2 and monomeric CH3 domain, have been engineered into scaffolds with favorable biophysical properties and a high potential for de novo antigen recognition...
November 14, 2016: Current Pharmaceutical Biotechnology
https://www.readbyqxmd.com/read/27834133/the-effects-of-colchicum-baytopiorum-on-regulatory-genes-of-apoptotic-and-autophagic-cell-death-in-hela-cells
#6
Özlem D Özsöylemez, Melek Öztürk, Nurhayat Sütlüpinar, Sera Kayacan, Matem Tunçdemir, Gül Özcan
BACKGROUND: The natural products have increasing important for the development of anticancer agents. Colchicum baytopiorum C.D.Brickell (C. baytopiorum), an endemic species for Turkey, contains colchicine and its derivatives. Stimulation of apoptotic and autophagy-mediated cell deaths are effective strategy for anticancer therapies. AIM: The aim of the study is to determine the role of the extract on both apoptotic and autophagic cell death in HeLa cell line. METHODS: The cell viability of C...
November 9, 2016: Current Pharmaceutical Biotechnology
https://www.readbyqxmd.com/read/27804852/design-synthesis-and-evaluation-of-heat-shock-protein-90-inhibitors-in-human-breast-cancer-and-its-metastasis
#7
Mehmet Gumus, Aykut Özgür, Lütfi Tutar, Ali Disli, İrfan Koca, Yusuf Tutar
In the present study, a series of benzodiazepine derivatives were designed and synthesized as novel heat shock protein 90 (Hsp90) inhibitor for target specific cancer treatment. Their anticancer activities were determined by XTT cell proliferation assay against human breast cancer cell line (MCF-7). Also, we investigated the antiangiogenic effects of the compounds on human vascular endothelium (HUVEC) cell line. In order to determine the anti-proliferative mechanism of the compounds, in silico molecular docking studies were performed between Hsp90 ATPase domain and benzodiazepine derivatives...
October 31, 2016: Current Pharmaceutical Biotechnology
https://www.readbyqxmd.com/read/27804853/glyco-engineering-of-human-igg-fc-to-modulate-biologic-activities
#8
Roy Jefferis
Advances in genetic and protein engineering and the ability to maintain proliferating mammalian cells in vitro, has allowed reverse engineering of antibodies, i.e. generation of antibodies having specificity for self-antigens. Thus, the lethal consequence of horror autotoxicus, anti-self-responses as envisaged by Paul Ehrlich (1854-1915), has been turned to advantage for treatment of multiple disease states. In order to reap these benefits it is essential that, in addition to target specificity, the antibody is customised to deliver appropriate downstream biologic effector activities...
October 29, 2016: Current Pharmaceutical Biotechnology
https://www.readbyqxmd.com/read/27804854/aerosol-delivery-devices-for-the-treatment-of-adult-patients-in-acute-and-critical-care
#9
Arzu Ari, James B Fink
Aerosolized medications are commonly used for the treatment of patients with pulmonary diseases in acute care. While there are many drug/device combinations available on the market, new devices have been developed in the past few years. Due to differences in their operation, performance and requirements for proper use, it is important to know how to use these aerosol devices effectively during inhalation therapy both in ambulatory and acute settings. This paper reviews aerosol devices used for the treatment of patients with an emphasis on acute and critical care...
October 28, 2016: Current Pharmaceutical Biotechnology
https://www.readbyqxmd.com/read/27784221/bio-fabrication-of-silver-nanoparticle-from-argemone-mexicana-for-the-control-of-aedes-albopictus-and-their-antimicrobial-activity
#10
Siva Kamalakannan, Sivapunyam Ananth, Kadarkarai Murugan, Kalimuthu Kovendan, Marimuthu Ramar, Ponnan Arumugam, Balamurugan Chandramohan, Vellingiri Balachandar
Plant synthesized nanoparticles gives rapid control of mosquito larvae of dengue vector, Aedes albopictus. Hence, the present study evaluated AgNPs synthesized from the plant, Argemone mexicana for the control of larvae and these nanoparticle were subjected to analyzed by UV-vis Spectrophotometry, Fourier transform infrared spectroscopy, scanning electron microscopy, and transmission electron microscopy. Furthermore, laboratory evaluation of plant mediated nano-particle was carried out lethal toxicity on Aedes albopictus...
October 25, 2016: Current Pharmaceutical Biotechnology
https://www.readbyqxmd.com/read/27774889/antibacterial-effect-of-gallic-acid-against-aeromonas-hydrophila-and-aeromonas-sobria-through-damaging-membrane-integrity
#11
Jing Lu, Zhenning Wang, Mengrou Ren, Guoren Huang, Baochen Fang, Xiujuan Bu, Yanhui Liu, Shuang Guan
In the study, we investigated the antibacterial activity and mechanism of gallic acid against Aeromonas hydrophila and Aeromonas sobria. Gallic acid showed strong antimicrobial activity against the two bacteria. Furthermore, the antibacterial mechanism of gallic acid (0, 3, 6, 12 mM ) was performed by membrane integrity assay and scanning electron microscopy (SEM) assay. The results showed that gallic acid notably increased the released material absorption value at 260, 280 nm and electric conductivity in a dose-dependent manner...
October 22, 2016: Current Pharmaceutical Biotechnology
https://www.readbyqxmd.com/read/27774891/evaluation-of-antioxidant-activity-of-phytol-using-non-and-pre-clinical-models
#12
Jéssica P Costa, Md T Ul-Islam, Pauline S Santos, Paula B Ferreira, George L S Oliveira, Marcus V O B Alancer, Marcia F C J Paz, Éverton L F Ferreira, Chistiane M Feitosa, Antonia M G L Citó, Damião P Sousa, Ana Amelia C Melo-Cavalcante
Phytol (3,7,11,15-tetramethylhexadec-2-en-1-ol; PHY), the alcoholic diterpenoid is particularly interesting due to its diverse activities found in literature. This study evaluated in vitro and in vivo antioxidant capacity of PHY. For this we conducted DPPH• (2,2-diphenyl-1-picrylhydrazyl) and ABTS•+ (2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid)) radical scavenging tests as in vitro, while Saccharomyces cerevisiae test as in vivo. For in vitro tests, trolox and for in vivo test hydrogen peroxide (H2O2) were taken as standard and stressor, respectively...
October 19, 2016: Current Pharmaceutical Biotechnology
https://www.readbyqxmd.com/read/27774890/a-new-era-for-cancer-target-therapies-applying-systems-biology-and-computer-aided-drug-design-to-cancer-therapies
#13
Yung-Hao Wong, Chia-Chiun Chiu, Chih-Lung Lin, Ting-Shou Chen, Bo-Ren Jheng, Yu-Ching Lee, Jeremy Chen, Bor-Sen Chen
In recent years, many systems biology approaches have been used with various cancers. The materials described here can be used to build bases to discover novel cancer therapy targets in connection with computer-aided drug design (CADD). A deeper understanding of the mechanisms of cancer will provide more choices and correct strategies in the development of multiple target drug therapies, which is quite different from the traditional cancer single target therapy. Targeted therapy is one of the most powerful strategies against cancer and can also be applied to other diseases...
October 19, 2016: Current Pharmaceutical Biotechnology
https://www.readbyqxmd.com/read/27758689/mechanisms-of-anorexia-cancer-cachexia-syndrome-and-potential-benefits-of-traditional-medicine-and-natural-herbs
#14
Cong Ming-Hua, Zou Bao-Hua, Yu Lei
Anorexia cancer cachexia syndrome is prevalent in advanced cancer patients, which is featured by anorexia, decreased dietary intake, body weight loss (skeletal muscle mass loss), and unable to be reversed by routine nutritional support therapy. Up to now, the main mechanisms involved in cancer cachexia include excessive systemic inflammation, which is represented by increased plasma levels of IL-1, IL-6, TNF-alpha, tumor-induced factors, such as PIF and LMF. These factors eventually act on orexigenic and anorexigenic neurons located in hypothalamus or protein and lipid metabolism of peripheral tissues, which lead to anorexia, decreased dietary intake, enhanced basic metabolism rate and hyper catabolism...
October 18, 2016: Current Pharmaceutical Biotechnology
https://www.readbyqxmd.com/read/27758688/generation-of-bispecific-antibodies-by-fc-heterodimerization-and-their-application
#15
Zhizhen Chen, Liping Wang, Tianshu Xu, Qifei Wang, Lishan Kang, Qi Zhao
Bispecific antibodies with binding specificities for two different antigens have prompted a lot of interest into their development and application. Currently, more than ten bispecific antibodies have been clinically validated for the treatment of various diseases, including cancers and inflammatory diseases. Intensive studies in antibody engineering drive the generation of different bispecific antibody formats that differ in size and shape. However, the most prominent formats, such as IgG-single-chain (sc) Fv or dual-variable domain (DVD) IgG, deviating from the natural IgG structure, may lead to manufacturing difficulties or increase the potential risk of immunogenicity...
October 18, 2016: Current Pharmaceutical Biotechnology
https://www.readbyqxmd.com/read/27677273/simultaneous-determination-of-cytochrome-p450-oxidation-capacity-in-humans-a-review-on-the-phenotyping-cocktail-approach
#16
Fernando de Andrés, Adrián Llerena
Intra- and/or inter-individual variability in drug response is mainly a result of either sub-therapeutic or supratherapeutic plasma levels of the active drugs and their metabolites, with this variability mainly being influenced by differences in the rate of drug metabolism. Indeed, drug metabolism is largely determined by genetic polymorphism in the CYP enzymes, which are responsible for approximately 85% of the drug metabolism process. However, this genetic heterogeneity can accurately predict actual drug metabolizing capacity (oxidation phenotype) for some individuals: poor metabolizers (PMs), who cannot produce the drug metabolizing enzymes, and 20% of ultra-rapid metabolizers...
September 26, 2016: Current Pharmaceutical Biotechnology
https://www.readbyqxmd.com/read/27658618/astaxanthin-a-promising-protector-against-gentamicin-induced-nephrotoxicity-in-rats
#17
Yasser Omar, Naglaa Abd El Khalik Gobba, Mohammed Abdalla Hussein
Gentamicin is an aminoglycoside antibiotic widely used against infections caused by Gram-negative microorganisms. Nephrotoxicity is the main limitation to its therapeutic use. The objective of this study was to evaluate the potential protective effect of astaxanthin on the renal damage generated by gentamicin in rats, in an attempt to understand its mechanism of action, which may pave the way for possible therapeutic applications. The daily oral administration of the astaxanthin at a concentration of 50 mg/kg for 15 days to gentamicin (80 mg/kg...
September 22, 2016: Current Pharmaceutical Biotechnology
https://www.readbyqxmd.com/read/27655361/n-butyl-%C3%AE-d-fructofuranoside-isolated-from-ulmus-davidiana-enhances-nrf2-activity-through-activation-of-jnk
#18
Hee-Jin Choi, Hee-Jung Choi, Mi-Joo Park, Tae-Wook Chung, Myungsoo Joo, Cheorl-Ho Kim, Hyun-Wook Chang, Jong-Keun Son, Ki-Tae Ha
BACKGROUND: The root barks of Ulmus davidiana Nakai (Ulmaceae) is a traditional Korean medicinal plant used for treating inflammatory disease. OBJECTIVE: We investigated the Nrf2 activating effect of U. davidiana and founded a novel candidate for Nrf2 activator from its ingredient compounds. METHODS: The cytotoxicity was measured by MTT assay. The Nrf2 activity was examined by luciferase-reporter assay and western blot analysis. The expression of Nrf2-dependent anti-oxidant genes was estimated by RT-PCR...
September 20, 2016: Current Pharmaceutical Biotechnology
https://www.readbyqxmd.com/read/27655363/stereospermum-fimbriatum-as-a-potential-source-of-phytochemicals-a-review-of-stereospermum-genus
#19
Anis Fadhlina Izyani Awang, Sahena Ferdosh, Zaidul Islam Sarker, Hassan Ibrahim Sheikh, Kashif Ghafoor, Kamaruzzaman Yunus
Stereospermum fimbriatum is one of medicinal plants that has been claimed to be used traditionally to treat several illnesses such as stomachache, earache, skin irritation and postpartum illness. The genus of this plant is known to possess medicinal properties in every part of the plant. Therapeutic potential of S. fimbriatum is anticipated based on numerous previous studies that documented variety of phytochemical contents and bioactivity of the genus. The most reported bioactivities of its genus are antimicrobial, antioxidant, anti-diabetic, anti-inflammatory, anti-diarrheal and analgesic activity...
September 19, 2016: Current Pharmaceutical Biotechnology
https://www.readbyqxmd.com/read/27655362/optimization-of-ultrasonic-microwave-synergistic-extraction-of-ricinine-from-castor-cake-by-response-surface-methodology
#20
Wei Xu, Xiuhua Yan, Rong Shao, Ligen Chen, Zengguang Ke
Castor cake is the residue in castor oil production in which many active components exist and the major one of which is ricinine. In this study, optimization of extraction of ricinine from castor cake using ultrasonic-microwave synergistic extraction (UMSE) was investigated to obtain high yield and purity by Box-Behnken design (BBD) response surface design. The optimal condition of extraction were achieved: ultrasound power 342 W, extracting time 5 min, microwave power 395 W, non significant factor of liquid/solid ratio 1:10...
September 19, 2016: Current Pharmaceutical Biotechnology
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