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Animal Science Journal, Nihon Chikusan Gakkaihō

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https://www.readbyqxmd.com/read/28631434/survival-of-spray-dried-and-free-cells-of-potential-probiotic-lactobacillus-plantarum-564-in-soft-goat-cheese
#1
Zorica Radulović, Jelena Miočinović, Nemanja Mirković, Milica Mirković, Dušanka Paunović, Marina Ivanović, Sanja Seratlić
A high viability of probiotics in food product, with a living cells threshold of 10(7) /cfu/g (colony-forming units/g) is a challenge to achieve in food production. Spray drying is an efficient and economic industrial method for probiotic bacterial preservation and its application in food products. In this study, the survival of free and spray-dried cells of potential probiotic strain Lactobacillus plantarum 564 after production and during 8 weeks of storage of soft acid coagulated goat cheese was investigated, as well as compositional and sensory quality of cheese...
June 19, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28631391/myosin-substitution-rate-is-affected-by-the-amount-of-cytosolic-myosin-in-cultured-muscle-cells
#2
Koichi Ojima, Emi Ichimura, Yuya Yasukawa, Mika Oe, Susumu Muroya, Takahiro Suzuki, Jun-Ichi Wakamatsu, Takanori Nishimura
In striated muscles, approximately 300 myosin molecules form a single thick filament in myofibrils. Each myosin is continuously displaced by another myosin to maintain the thick filament structure. Our previous study using a fluorescence recovery after photobleaching (FRAP) technique showed that the myosin replacement rate is decreased by inhibition of protein synthesis, but myosin is still exchangeable. This result prompted us to examine whether myosin in the cytoplasm is involved in myosin replacement in myofibrils...
June 19, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28631344/evaluation-of-the-genetic-structure-of-sika-deer-cervus-nippon-in-japan-s-kanto-and-tanzawa-mountain-areas-based-on-microsatellite-markers
#3
Sayaka Konishi, Shoko Hata, Sayumi Matsuda, Kazushi Arai, Yasushi Mizoguchi
The browsing habits of sika deer (Cervus nippon) in Japan have caused serious ecological problems. Appropriate management of sika deer populations requires understanding the different genetic structures of local populations. In the present study, we used 10 microsatellite polymorphisms to explore the genetic structures of sika deer populations (162 individuals) living in the Kanto region. The expected heterozygosity of the Tanzawa mountain range population (Group I) was lower than that of the populations in the Kanto mountain areas (Group II)...
June 19, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28594136/extension-of-the-culture-period-for-the-in%C3%A2-vitro-growth-of-bovine-oocytes-in-the-presence-of-bone-morphogenetic-protein-4-increases-oocyte-diameter-but-impairs-subsequent-developmental-competence
#4
Yinghua Yang, Chihiro Kanno, Kenichiro Sakaguchi, Yojiro Yanagawa, Seiji Katagiri, Masashi Nagano
Bone morphogenetic protein-4 (BMP-4) inhibits luteinization of granulosa cells during in vitro growth (IVG) culture of bovine oocytes; however, oocytes derived from a 12 day IVG were less competent for development than in vivo-grown oocytes. We herein investigated whether an extended IVG culture with BMP-4 improves oocyte growth and development to blastocysts after in vitro fertilization. Oocyte-granulosa cell complexes (OGCs) were cultured for 14 or 16 days with BMP-4 (10 ng/mL), while a 12 day culture with BMP-4 served as the in vitro control...
June 8, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28594135/the-igf-1-akt-s6-pathway-and-expressions-of-glycolytic-myosin-heavy-chain-isoforms-are-upregulated-in-chicken-skeletal-muscle-during-the-first-week-after-hatching
#5
Takaoki Saneyasu, Tatsuya Tsuchihashi, Ayana Kitashiro, Nami Tsuchii, Sayaka Kimura, Kazuhisa Honda, Hiroshi Kamisoyama
Skeletal muscle mass is an important trait in the animal industry. We previously reported an age-dependent downregulation of the insulin-like growth factor 1 (IGF-1)/Akt/S6 pathway, major protein synthesis pathway, in chicken breast muscle after 1 week of age, despite a continuous increase of breast muscle weight. Myosin heavy chain (HC), a major protein in muscle fiber, has several isoforms depending on chicken skeletal muscle types. HC I (fast-twitch glycolytic type) is known to be expressed in adult chicken breast muscle...
June 8, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28594103/dietary-%C3%AE-ketoglutarate-supplementation-improves-hepatic-and-intestinal-energy-status-and-anti-oxidative-capacity-of-cherry-valley-ducks
#6
Shuangshuang Guo, Rui Duan, Lei Wang, Yongqing Hou, Linglin Tan, Qiang Cheng, Man Liao, Binying Ding
α-Ketoglutarate (AKG) is an extensively used dietary supplement in human and animal nutrition. The aim of the present study was to investigate effects of dietary AKG supplementation on the energy status and anti-oxidative capacity in liver and intestinal mucosa of Cherry Valley ducks. A total of 80 1-day-old ducks were randomly assigned into four groups, in which ducks were fed basal diets supplemented with 0% (control), 0.5%, 1.0% and 1.5% AKG, respectively. Graded doses of AKG supplementation linearly decreased the ratio of adenosine monophosphate (AMP) to adenosine triphosphate (ATP) in the liver, but increased ATP content and adenylate energy charge (AEC) in a quadratic and linear manner, respectively (P < 0...
June 8, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28594101/effects-of-different-n-6-to-n-3-polyunsaturated-fatty-acids-ratio-on-reproductive-performance-fecal-microbiota-and-nutrient-digestibility-of-gestation-lactating-sows-and-suckling-piglets
#7
Jia Yin, Kwang Yong Lee, Jong Keun Kim, In Ho Kim
This study was conducted to evaluate the effects of dietary ratios of n-6:n-3 polyunsaturated fatty acids (PUFA) on reproductive performance, fecal microbiota and nutrient digestibility of gestation-lactating sows and suckling piglets. Fifteen primiparous sows (Landrace × Yorkshire) were randomly allotted into three treatments. Fed diets contained different ratios of n-6:n-3 PUFA, including 20:1, 15:1 and 10:1. No differences were detected among the treatments for average daily feed intake (ADFI) of sows and the back fat levels during lactation (P > 0...
June 8, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28594075/influence-of-dietary-fat-source-on-sow-and-litter-performance-colostrum-and-milk-fatty-acid-profile-in-late-gestation-and-lactation
#8
Chao Jin, Zhengfeng Fang, Yan Lin, Lianqiang Che, Caimei Wu, Shengyu Xu, Bin Feng, Jian Li, De Wu
The objective of this study was to investigate the effects of dietary supplementation with various fat sources (3.8-3.9% of diet) during late pregnancy and lactation on the reproductive performance, fatty acids profile in colostrum, milk and serum of sow progeny. A total of 80 multiparous sows were randomly fed a control (adding no oil), palm oil (PO), fish oil (FO) or soybean oil (SO) supplemented diet from 90 days of pregnancy to weaning. Supplementation of FO increased litter size of weak piglets, compared with the control-fed sows (P < 0...
June 8, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28593730/dynamics-of-cytokine-gene-expression-in-peripheral-blood-mononuclear-cells-of-indigenous-and-exotic-breeds-of-pigs-in-india
#9
Perumalraja Kirthika, Mohammad Ayub Ali, Parthasarathi Behera, Prasant Kumar Subudhi, Thingujam Chaa Tolenkhomba, Jagan Mohanarao Gali
To incorporate immune competence traits in swine breeding programs, association between immune responsiveness and susceptibility to specific infectious diseases must be established. In order to understand the differences in immune competence between indigenous (Zovawk) and exotic (Large White Yorkshire: LWY) pigs reared in India, we carried out a time course expression analysis of immune-regulating key cytokine genes (interleukin (IL)-2, interferon (IFN)-γ, IL-4 and IL-10) in the phytohemagglutinin-P stimulated peripheral blood mononuclear cells (PBMCs)...
June 8, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28585780/the-effects-of-l-dopa-and-sulpiride-on-growth-hormone-secretion-at-different-injection-times-in-holstein-steers
#10
Etsuko Kasuya, Madoka Sutoh, Ken-Ichi Yayou
The effects of l-DOPA, a precursor of dopamine (DA), and sulpiride, a D2 -type DA receptor blocker, on growth hormone (GH) and prolactin (PRL) secretion were investigated in steers. Eight Holstein steers (212.8 ± 7.8 kg body weight) were used. Lighting conditions were 12:12 L:D (lights on: 06.00-18.00 hours). Blood samplings were performed during the daytime (11.00-15.00 hours) and nighttime (23.00-03.00 hours). Intravenous injections of drugs or saline were performed at 12.00 hour for the daytime and 00...
June 6, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28585769/effects-of-transforming-growth-factor-%C3%AE-1-treatment-on-muscle-regeneration-and-adipogenesis-in-glycerol-injured-muscle
#11
Mohamed A A Mahdy, Katsuhiko Warita, Yoshinao Z Hosaka
Transforming growth factor (TGF)-β1 is associated with fibrosis in many organs. Recent studies demonstrated that delivery of TGF-β1 into chemically injured muscle enhances fibrosis. In this study, we investigated the effects of exogenous TGF-β1 on muscle regeneration and adipogenesis in glycerol-injured muscle of normal mice. Tibialis anterior (TA) muscles were injured by glycerol injection. TGF-β1 was either co-injected with glycerol, as an 'early treatment' group, or injected at day 4 after glycerol, as a 'late treatment' group and the TA muscles were collected at day 7 after initial injury...
June 6, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28574635/estradiol-inhibits-hepatic-stellate-cell-area-and-collagen-synthesis-in-the-chicken-liver
#12
Shotaro Nishimura, Akifumi Teshima, Fuminori Kawabata, Shoji Tabata
Hepatic stellate cells (HSCs) are the main collagen-producing cells in the liver. The HSC area and amount of collagen fibers are different between male and female chickens. This study was performed to confirm the effect of estradiol on collagen synthesis in the growing chicken liver. Blood estradiol levels in chicks were compared at 4 and 8 weeks of age, and the collagen fibril network in liver tissue was observed at 8 weeks by scanning electron microscopy. Intraperitoneal administrations of estradiol and tamoxifen to male and female chicks, respectively, were performed daily from 5 to 8 weeks of age...
June 2, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28574624/proper-reprogramming-of-imprinted-and-non-imprinted-genes-in-cloned-cattle-gametogenesis
#13
Masahiro Kaneda, Shinya Watanabe, Satoshi Akagi, Yasushi Inaba, Masaya Geshi, Takashi Nagai
Epigenetic abnormalities in cloned animals are caused by incomplete reprogramming of the donor nucleus during the nuclear transfer step (first reprogramming). However, during the second reprogramming step that occurs only in the germline cells, epigenetic errors not corrected during the first step are repaired. Consequently, epigenetic abnormalities in the somatic cells of cloned animals should be erased in their spermatozoa or oocytes. This is supported by the fact that offspring from cloned animals do not exhibit defects at birth or during postnatal development...
June 2, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28568977/reproductive-performance-and-expression-of-imprinted-genes-in-somatic-cell-cloned-boars
#14
Tatsuo Kawarasaki, Satoko Enya, Masayoshi Otake, Masatoshi Shibata, Satoshi Mikawa
To assess the performance of boars derived by somatic cell cloning, we analyzed various aspects of their reproductive characteristics and the expression of two imprinted genes. Cloned boars (cloned Duroc × Jinhua) were analyzed for birth weight, growth rate, age at first ejaculation, semen characteristics and fertility, in comparison with naturally bred control boars of the same strain. The expression of imprinted genes was analyzed using the microsatellite marker SWC9 for the paternally expressed gene insulin-like growth factor -2 (IGF2) and with single nucleotide polymorphisms (SNPs) for the gene maternally expressed 3 (MEG3)...
June 1, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28568309/effect-of-soybean-husk-supplementation-on-the-fecal-fermentation-metabolites-and-microbiota-of-dogs
#15
Htun Myint, Yu Iwahashi, Satoshi Koike, Yasuo Kobayashi
In vitro fermentation and in vivo feeding experiments were conducted to characterize the effects of soybean (Glycine max) husk on the fecal fermentation metabolites and microbiota of dogs. An in vitro fermentation study using feces from three Toy Poodle dogs (6.5 ± 3.5 months in age and 2.9 ± 0.4 kg in body weight) revealed that the fecal inoculum was able to ferment soybean husk (supplemented at 0.01 g/mL culture) and increased levels of short chain fatty acids (SCFA) and Bifidobacterium, irrespective of pre-digestion of the husk by pepsin and pancreatin...
June 1, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28560860/the-intrinsic-cause-of-color-fading-in-sliced-cooked-cured-beef-during-chilled-storage
#16
Jing Ma, Chensha Yu, Juanjuan Guo, Mengting Wu, Yunqiang Xu, Hongchao Yi, Weiqing Sun
The relationship between color change and other physical and chemical characteristics of sliced cooked cured beef (SCCB) during chilled storage were investigated using principal components analysis (PCA) to determine the color fading causes. Samples were prepared and stored at 8°C for up to 35 days in a vacuum package to simulate a supermarket storage environment; related indicators were measured periodically every week. The results showed that the first PC explained 59.82% of the total variation, and the second explained 22...
May 30, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28560744/silage-preparation-and-fermentation-quality-of-kudzu-sugarcane-top-and-their-mixture-treated-with-lactic-acid-bacteria-molasses-and-cellulase
#17
Xinzhu Chen, Wenyang Li, Chengfang Gao, Xiaopei Zhang, Boqi Weng, Yimin Cai
We studied silage fermentation of kudzu (KZ), sugarcane top (ST) and their mixtures treated with additives to be able to effectively use available local feed resources. The silages were prepared using KZ, ST, KZ 90% + ST 10%, KZ 80% + ST 20%, KZ 70% + ST 30% and KZ 60% + ST 40%, based on fresh matter (FM). These silages were treated with 10(8)  colony-forming units/g epiphytic lactic acid bacteria (LAB), 5% molasses and 0.02% cellulase of FM. The KZ contained higher crude protein (CP; 14.52%) content and lower levels of neutral detergent fiber (NDF; 62...
May 30, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28558419/generation-of-recombinant-bovine-interferon-tau-in-the-human-embryonic-kidney-cell-line-and-its-biological-activity
#18
Toru Takahashi, Ryosuke Sakumoto, Ken-Go Hayashi, Misa Hosoe, Junsuke Shirai, Kazuyoshi Hashizume
The objective of this study was to generate recombinant bovine interferon tau (rbIFNT) in mammalian hosts. The complementary DNA encoding bovine IFNT2 was cloned for the construction of pRcRSV-bIFNT2 expression vector. The expression vector was transfected to 293 cells. Transfected cells harboring expression vector were selected with G418. Highly expressing clonal line was adapted to serum-free suspension culture in a spinner flask. The recombinant protein had 24 kDa apparent molecular mass, suggesting being expressed as a glycoprotein, and was purified from serum-free conditioned medium by the combination of Diethylaminoethanol Sepharose ion exchange and Sephacryl S-200 HR gel filtration...
May 30, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28557191/effect-of-increasing-monensin-sodium-levels-in-diets-with-virginiamycin-on-the-finishing-of-nellore-cattle
#19
João Marcos B Benatti, João Alexandrino Alves Neto, Ivanna M de Oliveira, Flávio D de Resende, Gustavo R Siqueira
This study evaluated the effect of increasing levels of monensin sodium (MON) in diets with virginiamycin (VM) on the finishing of feedlot cattle. Two hundred and eighty intact male Nellore cattle (348 ± 32 kg body weight, 22 months) received one of the following five diets: control diet (without additives); diet containing VM (25 mg per kg dry matter) combined with 0 (MON0), 10 (MON10), 20 (MON20) or 30 (MON30) mg MON per kg dry matter. During adaptation (28 days), the MON0 diet increased dietary net energy for maintenance and gain compared to the control diet (P = 0...
May 30, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28557169/delicate-changes-of-bioapatite-mineral-in-pig-femur-with-addition-of-dietary-xylooligosaccharide-evidences-from-raman-spectroscopy-and-icp
#20
Shujie Wang, Penghao Zhang, Xiangfeng Kong, Shengda Xie, Qiao Li, Zhen Li, Zhenlei Zhou
Bone mineral is strongly correlated with performance and health of animal bodies. The mineral bioapatite (BAp) is the dominant component in bone tissue. This study investigated mineralogical changes of BAp in pig femur by Raman spectroscopy and inductively coupled plasma optical emission spectrometry (ICP-OES). The pigs had been raised with various xylooligosaccharide (XOS) additions at two stages of growth (growing and fattening periods). The results show that XOS can decrease the degree of carbonate substitution for PO4 in BAp mineral and improve the mineral's crystallinity...
May 30, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
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