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Fish Physiology and Biochemistry

Eszter Kása, Jelena Lujić, Zoran Marinović, Tímea Kollár, Gergely Bernáth, Zoltán Bokor, Béla Urbányi, Kinga Katalin Lefler, Dušan Jesenšek, Ákos Horváth
Vitrification was applied to the sperm of two endangered fish species of Soča River basin in Slovenia, the Adriatic grayling (Thymallus thymallus) and marble trout (Salmo marmoratus) following testing different cooling devices and vitrifying media. Sperm was collected, diluted in species-specific non-activating media containing cryoprotectants, and vitrified by plunging directly into liquid nitrogen without pre-cooling. Progressive motility, curvilinear velocity, and straightness of fresh and vitrified-warmed sperm were evaluated with computer-assisted sperm analysis (CASA)...
May 19, 2018: Fish Physiology and Biochemistry
Carine de Freitas Souza, Cristine Rampelotto, Bruno Bianchi Loureiro, Fernanda Alves Pereira, Adriane Erbice Bianchini, Carine Dahl Corcini, Antonio Sergio Varela Junior, Tatiana Emanuelli, Leila Picolli da Silva, Sílvio Teixeira da Costa, Kalyne Bertolin, Monique Tomazele Rovani, Paulo Bayard Dias Gonçalves, Berta Maria Heinzmann, Bernardo Baldisserotto
In aquaculture, nutrition and supplemented diets have been shown to affect broodstock reproductive performance. In this study, we investigated the effects of dietary supplementation with Cymbopogon flexuosus essential oil (CFEO) microcapsules on reproductive-related parameters in silver catfish (Rhamdia quelen) male broodfish. Adult male broodstocks were separated into three groups according to the concentrations of supplemented CFEO (0.0 = control; 1.0 or 3.0 mL per kg of diet). After 20 days under experimental conditions, the animals were euthanized and the gonads were harvested for gonadosomatic index, sperm analysis, oxidative stress, and histopathology; testosterone levels were measured in the plasma; gene expression of prl, smtl, pomca, and pomcb was assessed in the pituitary gland by real-time PCR...
May 18, 2018: Fish Physiology and Biochemistry
Hongwei Yan, Xufang Shen, Xin Cui, Yumeng Wu, Lianshun Wang, Lei Zhang, Qi Liu, Yusheng Jiang
Quantifying the expression of mRNAs in the gonads at the critical stage of molecular sex differentiation stage might help to clarify the regulatory network during early sex differentiation and provide new information on the role of sex-related genes in gonadal function. In this study, transcriptomic analysis of sex-related genes expression profiles in fugu gonads at 60 and 90 days after hatching (dah) was conducted firstly, and a total of 112,504,991 clean reads, encompassing 28.35 Gb of sequences were retrieved...
May 18, 2018: Fish Physiology and Biochemistry
Ewa Ocłoń, Gili Solomon, Zvi Hayouka, Arieh Gertler
Fish growth hormones (GHs) play an important role in regulating growth, metabolism, reproduction, osmoregulation, and immunity and have thus garnered attention for their application in aquaculture. Zebrafish GH (zGH) cDNA or rainbow trout GH (rtGH) cDNA was cloned into the pMon3401 vector, expressed in MON105-competent Escherichia coli and purified to homogeneity. Their biological activity was evidenced by their ability to interact with ovine GH receptor extracellular domain and stimulate GH receptor-mediated proliferation in FDC-P1-3B9 cells stably transfected with rabbit GH receptor...
May 18, 2018: Fish Physiology and Biochemistry
Jelena Lujić, Zoran Marinović, Simona Sušnik Bajec, Ida Djurdjevič, Béla Urbányi, Ákos Horváth
Interspecific transplantation of germ cells from the brown trout Salmo trutta m. fario and the European grayling Thymallus thymallus into rainbow trout Oncorhynchus mykiss recipients was carried out in order to improve current practices in conservation of genetic resources of endangered salmonid species in the Balkan Peninsula. Current conservation methods mainly include in situ efforts such as the maintenance of purebred individuals in isolated streams and restocking with purebred fingerlings; however, additional ex situ strategies such as surrogate production are needed...
May 13, 2018: Fish Physiology and Biochemistry
Patrick G Pumputis, Vivian R Dayeh, Lucy E J Lee, Phuc H Pham, Zhenzhen Liu, Senthuri Viththiyapaskaran, Niels C Bols
In order to develop an in vitro system to study the cell biology of starvation in the fish intestine, rainbow trout intestinal epithelial cells were subjected to three kinds of nutrient deprivation and evaluated for 7 days. The RTgutGC cell line was grown into monolayers in Leibovitz's basal medium supplemented with fetal bovine serum (L15/FBS) and then subjected to deprivation of serum (L15); of serum, amino acids, and vitamin (L15/ex); and of all nutrients (L15/salts). After 7 days of nutrient deprivation, the cells remained attached to the plastic surface as monolayers but changes were seen in shape, with the cells becoming more polygonal, actin and α-tubulin cytoskeleton organization, and in tight junction protein-1 (ZO-1) localization...
May 12, 2018: Fish Physiology and Biochemistry
Wan-Cun Cai, Wen-Bin Liu, Guang-Zhen Jiang, Kai-Zhou Wang, Cun-Xin Sun, Xiang-Fei Li
This study aimed to investigate the effects of lysine supplement on the growth performance of blunt snout bream Megalobrama amblycephala fed diets with fish meal (FM) replaced by rice protein concentrate (RPC) with the potential mechanisms characterized. Fish were fed three diets, including the FM diet (containing FM), the RPC diet (FM replaced by RPC), and the MRPC diet (the RPC diet supplemented with lysine) for 8 weeks. Weight gain, protein efficiency ratio, and nitrogen and energy utilization of fish fed the FM diet were all significantly higher than those of the RPC treatment, but they showed no statistical difference with those of the MRPC group...
May 5, 2018: Fish Physiology and Biochemistry
Xiao-Chen Shi, Jian Sun, Ai Jin, Hong Ji, Hai-Bo Yu, Yang Li, Xue-Xian Li, Pin Liu, Chao Li, Ji-Qin Huang
Cytochrome P450 enzymes (CYP enzymes) catalyze important metabolic reactions of exogenous and endogenous substrates, including fatty acid. In this study, we cloned the complete CDS of the cytochrome P450 2AA (CYP2AA) gene from the grass carp (Ctenopharyngodon idella) for the first time. CYP2AA consisted of 1500 bp, which encoded a predicted protein of 499 amino acids. The identities of CYP2AA between C. idella and zebrafish were 86%. It consists of the conserved heme-binding motif FXXGXXXCXG. Quantitative real-time PCR analysis indicated that CYP2AA mRNA in C...
May 3, 2018: Fish Physiology and Biochemistry
V Gallego, J G Herranz-Jusdado, C Rozenfeld, L Pérez, Juan F Asturiano
Fish sperm motility is nowadays considered the best sperm quality biomarker in fish, and can be evaluated both by subjective and computerized methods. With the aim to compare the precision and accuracy of both techniques, fish sperm samples were assessed by subjective methods and by a computer-assisted sperm analysis (CASA-Mot) system, and simultaneously by three different technicians with different degrees of expertise on the sperm quality analysis. Statistical dispersion parameters (CV, coefficient of variation; and RG, range) were estimated in order to determine the precision and accuracy of the techniques and the influence of laboratory staff on sperm motion assessments...
April 30, 2018: Fish Physiology and Biochemistry
Diógenes Henrique de Siqueira-Silva, Taiju Saito, Amanda Pereira Dos Santos-Silva, Raphael da Silva Costa, Martin Psenicka, George Shigueki Yasui
This review discusses the new biotechnological tools that are arising and promising for conservation and enhancement of fish production, mainly regarding the endangered and the most economically important species. Two main techniques, in particular, are available to avoid extinction of endangered fish species and to improve the production of commercial species. Germ cell transplantation technology includes a number of approaches that have been studied, such as the transplantation of embryo-to-embryo blastomere, embryo-to-embryo differentiated PGC, larvae to larvae and embryo differentiated PGC, transplantation of spermatogonia from adult to larvae or between adults, and oogonia transplantation...
April 29, 2018: Fish Physiology and Biochemistry
Yong-Jun Dai, Wen-Bin Liu, Xiang-Fei Li, Man Zhou, Chao Xu, Yu Qian, Guang-Zhen Jiang
The aims of the present study were to clone the full-length cDNA of adipose triglyceridelipase (ATGL) and to analyze its expression after lipopolysaccharide (LPS)-induced tumor necrosis factor alpha (TNF-α). The cDNA obtained covered 1801 bp with an open reading frame of 1500 bp encoding 499 amino acids. Sequence alignment and phylogenetic analysis show the best identity with Cyprinus carpio (86%). The ATGL protein shared a highly conserved 169-amino acid patatin domain, containing a glycine-rich motif, an active serine hydrolase motif, and an aspartic active site...
April 29, 2018: Fish Physiology and Biochemistry
Yaling Sun, Yingwen Li, Zhihao Liu, Qiliang Chen
Mercury (Hg) is one of the most toxic heavy metals that can cause severe damage to fish. Studies have demonstrated that Hg has a specific affinity for the endocrine system, but little is known about the effects of Hg on thyroid endocrine system in fish. In this study, zebrafish embryos were exposed to environmentally relevant concentrations of 1, 4, and 16 μg/L Hg2+ (added as HgCl2 ) from 2 h post-fertilization (hpf) to 168 hpf. Thyroid hormone (TH) levels and mRNA expression levels of genes involved in the hypothalamus-pituitary-thyroid (HPT) axis were determined...
April 24, 2018: Fish Physiology and Biochemistry
Chenglong Wu, Jun'e Gao, Lian Chen, Xianping Shao, Jinyun Ye
In the present study, three cDNAs of AdipoRs (MpAdipoR1a, MpAdipoR1b, and MpAdipoR2) were identified from juvenile black carp Mylopharyngodon piceus. There were 375, 378, and 356 amino acids in the MpAdipoR1a, MpAdipoR1b, and MpAdipoR2, respectively. BLAST analysis reveals that MpAdipoRs share high identities with other known AdipoRs from zebrafish, rainbow trout, human, etc. And there were all seven transmembrane regions in the amino acid sequences of MpAdipoR1s and MpAdipoR2, respectively. The relative expression levels of MpAdipoR1s were higher in the liver, blood, brain, and eyes in black carp (p < 0...
April 23, 2018: Fish Physiology and Biochemistry
Sadegh Saffari, Saeed Keyvanshokooh, Mohammad Zakeri, Seyed Ali Johari, Hossein Pasha-Zanoosi, Mansour Torfi Mozanzadeh
An 8-week feeding trial was conducted to compare the effects of supplementing (0.7 mg kg-1 ) different dietary selenium (Se) sources including organic [selenomethionine (SeMet)], inorganic [sodium selenite (Na2 SeO3 )], and nanoparticulate Se (nano-Se) on physiological responses of common carp, Cyprinus carpio juveniles (9.7 ± 0.1 g). Basal diet without Se supplementation used as control. Fish fed nano-Se supplemented diet had the highest weight gain (97.2 ± 10.8%) and feed efficiency ratio (42...
April 16, 2018: Fish Physiology and Biochemistry
Patrícia Diogo, Gil Martins, Isa Quinzico, Rita Nogueira, Paulo J Gavaia, Elsa Cabrita
Zebrafish sperm cryopreservation is a fundamental methodology to manage and back-up valuable genetic resources like transgenic and mutant strains. Cryopreservation usually requires liquid nitrogen for storage, which is expensive and hazardous. Our objective was to evaluate if electric ultrafreezers (- 150 °C) are a viable alternative for zebrafish sperm storage. Zebrafish sperm was cryopreserved in the same conditions (- 20 °C/min), stored either in liquid nitrogen or in an ultrafreezer, and thawed after 1 week, 1 month, and 3 months...
April 13, 2018: Fish Physiology and Biochemistry
Maryam Ghiasi, Mohammad Binaii, Alireza Naghavi, Hosseinali Khoshbavar Rostami, Hossainali Nori, Atefeh Amerizadeh
Administration of probiotic candidates in fish has generally been shown as a useful strategy to improve growth performance, survival, digestive enzyme activity, and gut microbiota. Unfortunately, the sero-immunological responses of different fish to different probiotic candidates are poorly understood. The present study assessed the effect of Pediococcus acidilactici as a probiotic on the biochemical and immunological parameters of beluga. Fish (248.32 ± 10.21 g) were fed a control diet (without P. acidilactici( and three different doses of P...
April 10, 2018: Fish Physiology and Biochemistry
Müslüm Kuzu, Veysel Çomaklı, Ebru Akkemik, Mehmet Çiftci, Ömer İrfan Küfrevioğlu
In this study, CA I and II isoenzymes were purified from Van Lake fish gills by using Sepharose-4B-L-tyrosine-sulfanilamide affinity chromatography and to determine the effects of some metals on the enzyme activities. For purified CA I isoenzyme, yield, specific activity, and purification fold were obtained as 42.07%, 4948.12 EU/mg protein, and 116.61 and for CA II isoenzyme, 7%, 1798.56 EU/mg protein, and 42.38 respectively. Activity of CA was determined by measuring "CO2 -hydratase activity". Purity control was checked by SDS-PAGE...
April 8, 2018: Fish Physiology and Biochemistry
Katarzyna Lugowska
To determine the effects of Roundup, a commercial formulation of glyphosate, gametes, and embryos of common carp (Cyprinus carpio L) was exposed to wide range of herbicide concentrations (0.0, 0.1, 0.5, 2.0, 5.0, 10.0, 20.0, and 50.0 mg/l). The obtained results showed different effects of Roundup on common carp gametes. Herbicide reduced swelling of eggs (but the effect was not concentration-related), while sperm showed low sensitivity to Roundup (time of spermatozoa motility was reduced in a significant way only at 20 mg/l, and at remaining concentrations, only a slight tendency was observed)...
April 7, 2018: Fish Physiology and Biochemistry
Suktianchai Saekhow, Karun Thongprajukaew, Wutiporn Phromkunthong, Harit Sae-Khoo
Water volume is a key parameter affecting the individual rearing of male Siamese fighting fish (Betta splendens Regan, 1910). In this study, minimization of water volume was pursued by assessing growth, feed utilization, digestive enzyme activities, color coordinates, muscle quality, and carcass composition. One-month-old solid-red male fish (0.97 ± 0.01 g initial body weight) were distributed individually into glass aquaria with five alternative water volumes (100, 150, 200, 250, and 300 mL), comprising 15 fish per treatment (n = 15), over 8 weeks duration...
March 30, 2018: Fish Physiology and Biochemistry
Jinliang Du, Rui Jia, Li-Ping Cao, Weidong Ding, Pao Xu, Guojun Yin
Fatty liver is an increasingly serious disease of fish in aquaculture. However, the mechanisms responsible for the occurrence of fatty liver remain unclear, and no effective methods for the prevention and treatment of this disease have yet been found. In the present study, we aimed to develop an in vitro model of hepatocyte injury using oleic acid as hepatotoxicant and evaluate the protective effects of Rhizoma Alismatis extract (RAE) in Jian carp using this model. Primary hepatocytes from Jian carp were isolated and purified and cultured in vitro...
March 30, 2018: Fish Physiology and Biochemistry
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