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Fish Physiology and Biochemistry

Jing Xu, Fuyuan Hou, Debin Wang, Jun Li, Guangzhong Yang
Melanin-concentrating hormone (MCH) was initially known as a regulator of teleost skin color and possesses multiple functions in mammals, such as the regulation of energy balance and reproduction. However, the role of MCH in fish remains unclear. In the present study, a 590 bp cDNA fragment of common carp (Cyprinus carpio) MCH gene was cloned. Amino acid sequence similarities with other teleost ranged from 23 to 93%. The mature MCH peptide (DTMRCMVGRVYRPCWEV) located in the C-terminal region of MCH precursor was 100% identical to that of goldfish, zebrafish, chum salmon, and rainbow trout...
November 13, 2018: Fish Physiology and Biochemistry
Yan Chao, Mingzhe Xia, Rongrong Wu, Qichang Chen, Zhiqin Zheng, Delin Qi
Schizopygopsis pylzovi, an endemic fish of the subfamily Schizothoracinae, is comparatively well adapted to dissolved oxygen fluctuations in the aqueous environments of the Qinghai-Tibetan Plateau. Here, we cloned the complete cDNA of cytoglobin 1 and 2 (Cygb1 and Cygb2) from S. pylzovi and then investigated transcriptional changes of both genes in the selected tissues in response to hypoxia. Both the two genes had the standard exon-intron structure of vertebrate Mb genes but lacked an exon at downstream of the H helix (HC11...
November 7, 2018: Fish Physiology and Biochemistry
Laetitia Allais, Chao Zhao, Mingjun Fu, Jing Hu, Jian G Qin, Lihua Qiu, Zhenhua Ma
Understanding fish larval development is of a great interest for aquaculture production efficiency. Identifying possible indicators of fish larvae stress could improve the production and limit the mortality rate that larval stage is subjected to. Heat-shock proteins (HSPs) and heat-shock factors (HSFs) are well known as indicators of response to many kinds of stressor (e.g., environmental, morphological, or pathological changes). In this study, golden pompano larvae were raised at different temperatures (23 °C, 26 °C, and 29 °C), as well as three different diets (Artemia nauplii unenriched, Artemia nauplii enriched with Nannochloropsis sp...
November 5, 2018: Fish Physiology and Biochemistry
Ana Gabriela Jimenez, Evan Braun, Kailey Tobin
Estuarine environments are characterized by cyclical fluctuations in tides, with tidal shifts drastically, frequently, and acutely altering temperature, dissolved oxygen, and salinity. Despite these ecological challenges, the sheepshead minnow, Cyprinodon variegatus, seems to dominate estuarine landscapes. Here, we held sheepshead minnows to four temperature treatment groups for 1 month. We then tested whether temperature exposure had an effect on acute hypoxia tolerance via oxidative stress. We measured superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities, as well as total antioxidant capacity and lipid peroxidation (LPO) damage in white muscle...
November 5, 2018: Fish Physiology and Biochemistry
Ayyoub Jamali Kohshahi, Iman Sourinejad, Mehrdad Sarkheil, Seyed Ali Johari
The aim of this study was to investigate the effects of dietary selenium (nanoparticles, organic, and inorganic forms), curcumin (CUR), and their combination on survival, growth performance, body composition, innate immune responses, and glutathione peroxidase activity of rainbow trout (Oncorhynchus mykiss). CUR at level of 400 mg/kg dry diet and each of selenium nanoparticles (Se-NPs), organic selenium (Sel-Plax®), and sodium selenite at level of 1 mg/kg Se dry diet were added to basal diet. A total of 240 rainbow trout with mean initial weight of 14...
November 3, 2018: Fish Physiology and Biochemistry
Jin Wuk Lee, Jae-Woo Lee, Kyungtae Kim, Yu-Jin Shin, Jieun Kim, Hokyun Kim, Heejung Kim, Su-A Min, Pilje Kim, Kyunghee Choi, Kyunghwa Park
n-Butyl acrylate (nBA) is one of acrylate esters which has been applied to diverse industrial fields. For unveiling of xeno-estrogenic effects and oxidative stress induction by nBA under two-generational exposure regimen (17 weeks), the biomarkers relevant to an estrogenic effect and oxidative stress were analyzed. Acute toxicity value of nBA in Oryzias latipes was 7.2 mg/L (96 h-LC50). Over exposure time, the significant transcriptional change of cytochrome P450 19A (CYP19A) and vitellogenin 1/2 (VTG1/2) was not observed (one-way ANOVA, P < 0...
November 1, 2018: Fish Physiology and Biochemistry
Jun Liu, Juan-Juan Pang, Zong-Cai Tu, Hui Wang, Xiao-Mei Sha, Yan-Hong Shao, Guang-Xian Liu
Cadmium (Cd) is known to be a potentially toxic heavy metals to the fish health and growth. Carassius auratus gibelio (C. a. gibelio) specimens were exposed to waterborne Cd (0, 0.05, 0.10, 0.15, and 0.20 mg/L CdCl2 ) for 14 days. Cd accumulation, liver and intestine histopathology, and intestinal microorganism were investigated in the present study. The results indicated that Cd accumulation in the gill, liver, intestine, and muscle gradually decreased as Cd concentration increased. The gill accumulated higher amounts of Cd than other tissues...
November 1, 2018: Fish Physiology and Biochemistry
Leire Arantzamendi, Francisco Roo, Carmen María Hernández-Cruz, Hipólito Fernández-Palacios, Marisol Izquierdo
To characterise the progression of lipid digestion capacity in gilthead seabream across life cycle, the activities of bile salt-activated lipase (BAL) and phospholipase A2 (PLA2 ) were determined in the digestive tracts of cultured gilthead seabream from first feeding to marketable size (49 μg to 300 g). Four trials were undertaken with gilthead seabream of different ages, fed on diets with fishmeal and fish oil as the main dietary protein and lipid sources and 21-25% lipid contents. Larvae of 4 days after hatching (dah) to 9 dah were fed rotifers with different fatty acid profiles: control (2...
October 31, 2018: Fish Physiology and Biochemistry
Cristiano Campos Mattioli, Rodrigo Takata, Fabiola de Oliveira Paes Leme, Deliane Cristina Costa, Ronald Kennedy Luz
The present study aimed to evaluate the physiological and metabolic stress responses of juvenile Lophiosilurus alexandri submitted to an air exposure test. The subjects consisted of 72 juveniles. Blood samples were taken at: 0 h-fish not exposed to air; 0.5 h-fish shortly after exposure to air for 30 min (prior to returning to the tank); 1.5 h (90 min), 24, 48, and 96 h after the initiation of exposure to air for 30 min. After 96 h, survivorship was 100%. Cortisol and glucose levels were higher at 0...
October 27, 2018: Fish Physiology and Biochemistry
Elói Martins, Pedro R Almeida, Bernardo R Quintella, Marco Gomes da Silva, Maria João Lança
A comprehensive characterization of muscle's FA composition of sea lamprey ammocoetes and adults was performed to test the hypothesis that larvae, and early spawning migrants have a similar FA profile prior to metamorphosis and to spawning migration. Subsequently, the role played by FA signature in these two highly demanding stages of life cycle was inferred. The results confirm that muscle represents an important fat reservoir, and the FA trophic markers revealed the importance of bacteria as sources of iso and anteiso FA and the strong trophic representation of benthic phytoplankton (diatoms) to larvae muscle FA profile...
October 27, 2018: Fish Physiology and Biochemistry
Md Sakhawat Hossain, Shunsuke Koshio, Manabu Ishikawa, Saichiro Yokoyama, Nadia Mahjabin Sony, Md Jakiul Islam
We assessed the effects of fishmeal (FM) replacement by soy protein concentrate (SPC) with inosine monophosphate (IMP) supplementation on growth, digestibility, immunity, blood health, and stress resistance of red sea bream, Pagrus major. FM protein of a FM-based control diet (FM100) was replaced with 25 (FM75), 50 (FM50), 75 (FM25), and 100% (FM0) by SPC protein, and each replacement group was supplemented with 0.4% IMP to formulate five experimental diets. Each diet was randomly allocated to triplicate groups of fish (4...
October 26, 2018: Fish Physiology and Biochemistry
Carolina Castro, Ana Couto, Alexandre F Diógenes, Geneviève Corraze, Stéphane Panserat, Cláudia R Serra, Aires Oliva-Teles
For an increased incorporation of plant ingredients in aquafeeds at the expense of fish meal (FM) and fish oil (FO), more knowledge is needed on the effects at the intestine level of dietary vegetable oils (VO) and carbohydrates (CH), and of possible interactions. For that purpose, in this study, the activities of digestive pancreatic enzymes (amylase, lipase, total alkaline proteases), gut microbiota, and histomorphology were assessed in gilthead sea bream (IBW 71.0 ± 1.5 g) fed four diets differing in lipid source (FO or a blend of VO) and carbohydrate content (0% or 20% gelatinized starch) for 81 days...
October 26, 2018: Fish Physiology and Biochemistry
Wenbo Chen, Fangfang Yan, Shaozong Qin, Haiyan Dong
β-Thymosins play critical roles in the regulation of many important physiological processes, but their function in teleost fishes remains poorly understood. In this study, the full-length cDNA coding for a thymosin β (Tβ) was cloned and identified in goldfish, Carassius auratus (gfTβ). The gfTβ cDNA consisted of 653 bp with an open reading frame of 135 bp that encodes a 44 amino acid polypeptide. Sequence analysis revealed one thymosin domain and a highly conserved actin-binding motif (18 LKKTET23 )...
October 26, 2018: Fish Physiology and Biochemistry
Yu Jiang, Bin Chen, Yilin Yan, Guo-Xing Zhu
Iron overload increases the risk of osteoporosis, which leads to an increase in the incidences of bone fracture after menopause. In vitro studies have demonstrated that excess iron can inhibit osteoblast activity. Hepcidin, a central regulator of iron homeostasis, prevents iron overload, and thus, it is considered to have anti-osteoporosis effects. In this study, a zebrafish model was employed to investigate the therapeutic role of hepcidin in iron overload-induced inhibition of bone formation. Our results show that ferric ammonium citrate (FAC) treatment decreased osteoblast-specific gene expression (runx2a, runx2b, and bglap) and bone mineralization in the zebrafish embryo, accompanied with increased whole-body iron levels and oxidative stress...
October 25, 2018: Fish Physiology and Biochemistry
Nadia Mahjabin Sony, Manabu Ishikawa, Md Sakhawat Hossain, Shunsuke Koshio, Saichiro Yokoyama
We determined the supplementation effects of dietary fucoidan on growth, immune responses, blood characteristics, and oxidative stress resistance of juvenile red sea bream. A fishmeal (FM)-based basal diet supplemented with 0% (D1, control), 0.05% (D2), 0.1% (D3), 0.2% (D4), 0.4% (D5), and 0.8% (D6) mozuku fucoidan to formulate six experimental diets. Each diet was randomly allocated to triplicate groups of fish (3.8 g) for 60 days. Results showed that fish-fed diet D5 showed significantly higher (P < 0...
October 5, 2018: Fish Physiology and Biochemistry
Shengchen Liao, Jingjing Dong, Weina Xu, Bingwen Xi, Yaxiong Tao, Bo Liu, Jun Xie
The melanocortin-5 receptor (MC5R) plays an important role in the regulation of exocrine secretion in mammals. Its function in fish is not well established. In this study, we reported the molecular cloning, tissue expression, and pharmacological characterization of Megalobrama amblycephala MC5R (MamMC5R), as well as the effect of catching stress on its expression. The full-length cDNA of Mammc5r gene was 1237 bp, consisted of a 990-bp open reading frame encoding 329 amino acids. Sequence analyses revealed that the nucleotide and amino acid sequences of Mammc5r were highly homologous (> 90%) with MC5Rs of zebrafish, common carp, and goldfish...
October 4, 2018: Fish Physiology and Biochemistry
Edison Barbieri, Alessandra Maria Tegon Ferrarini, Karina Fernandes Oliveira Rezende, Diego Stéfani Teodoro Martinez, Oswaldo Luiz Alves
The study of the toxic effect of carbofuran and multiwalled carbon nanotubes (MWCNTs) on Astyanax ribeirae metabolism is of paramount importance due to the increasing use of this pesticide in agriculture and in the production of nanotubes within the material industry. This study aimed to evaluate the effects of carbofuran, MWCNT, and the combination of these compounds on specific oxygen consumption and excretion of ammonia in A. ribeirae. Therefore, 65 fish were divided into three groups of treatments at varying concentrations: carbofuran (0...
October 1, 2018: Fish Physiology and Biochemistry
Yongyi Jia, Jianbo Zheng, Meili Chi, Shili Liu, Wenping Jiang, Shun Cheng, Zhimi Gu, Liqiao Chen
Dmrt1, a member of the Dmrt family, is an important transcription regulator of gender determination. To study the biological function of dmrt1 in sexual differentiation and its potential implication in breeding technology, we obtained the full-length cDNA and proximal promoter sequence of dmrt1 in Culter alburnus, and analyzed the impact of promoter CpG methylation on the gene expression pattern of dmrt1 during gonad development. Dmrt1 was 922 bp in length and consisted a 150 bp 5'-UTR, a 28 bp 3'-UTR, and a 744 bp open reading frame (ORF)...
October 1, 2018: Fish Physiology and Biochemistry
Martin Pšenička
The elimination of egg stickiness is required for effective artificial reproduction of northern pike, but until now, available methods have required at least 40 min. Sodium hypochlorite was tested under laboratory conditions, and exposure to aqueous concentrations of 0.025-0.05% for 40 s effectively eliminated stickiness without adverse effects. Fertilization and hatching rates in laboratory trials were similar to those observed in eggs treated with traditional methods using clay or milk for 40 or 60 min, respectively, as well as those without treatment...
October 1, 2018: Fish Physiology and Biochemistry
F P Venturini, F D de Moraes, P A Rossi, I M Avilez, N S Shiogiri, G Moraes
Effects of the pyrethroid lambda-cyhalothrin (LCH) were investigated in matrinxa Brycon amazonicus, a non-target freshwater teleost. The fish were submitted to a single-pulse exposure (10% of LC50; 96 h, 0.65 μg L-1 ), followed by 7 days of recovery in clean water. Hematologic parameters indicated impairments in oxygen transport, which were not recovered. Plasma [Na+ ], [Cl- ], and protein were diminished, and only [Na+ ] remained low after recovery. Gill Na+ /K+ ATPase activity was increased and recovered to basal values...
September 30, 2018: Fish Physiology and Biochemistry
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