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Fish Physiology and Biochemistry

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https://www.readbyqxmd.com/read/28101704/molecular-cloning-and-gene-protein-expression-of-fat-cd36-from-grass-carp-ctenopharyngodon-idella-and-the-regulation-of-its-expression-by-dietary-energy
#1
Juan Tian, Wei Liu, Weihua Gao, Fan Wu, Lijuan Yu, Xing Lu, Chang-Geng Yang, Ming Jiang, Hua Wen
Fatty acid translocase/cluster of differentiation 36 (FAT/CD36) functions as a membrane long-chain fatty acid transporter in various tissues in land animals. Not much is known about the CD36 molecule in teleost fish. Therefore, we studied CD36 in grass carp (Ctenopharyngodon idella, ciCD36). The full-length complementary DNA sequence of ciCD36 was 1976 bp, with an ORF of 468 amino acids, which had high sequence similarity to the CD36 of common carp. The messenger RNA (mRNA) expression of ciCD36 was high in the intestine, heart, liver, visceral tissue, and brain, but absent in the kidney...
January 18, 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/28097495/the-expression-of-hypoxia-inducible-factor-1%C3%AE-gene-is-not-affected-by-low-oxygen-conditions-in-yellow-perch-perca-flavescens-juveniles
#2
Karolina Kwasek, Simona Rimoldi, Anna Giulia Cattaneo, Timothy Parker, Konrad Dabrowski, Genciana Terova
Hypoxia can affect various fish populations, including yellow perch Perca flavescens, which is an economically and ecologically important species in Lake Erie, a freshwater system that often experiences hypoxia in the hypolimnetic part of the lake. Fish, similarly to mammals, possess molecular oxygen sensor-hypoxia-inducible factor-1 (HIF-1), a transcription factor that can affect expression of many downstream genes related to animal growth and locomotion, protein synthesis, as well as ATP and amino acid metabolism...
January 18, 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/28097494/establishment-and-characterization-of-a-heart-derived-cell-line-from-goldfish-carassius-auratus
#3
Hongli Jing, Xiangmei Lin, Lipu Xu, Longying Gao, Min Zhang, Na Wang, Shaoqiang Wu
The goldfish Carassius auratus, a freshwater fish in the family Cyprinidae, was one of the earliest fish to be domesticated for ornamental purposes. A cell line was established from goldfish heart (GH) tissue to create a biological monitoring tool for viral diseases. The GH cell line was optimally maintained at 25 °C in M199 medium supplemented with 10-20% fetal bovine serum. A chromosomal analysis indicated that the cell line remained diploid, with a mean chromosomal count of 100. In viral inoculation assays, significant cytopathic effects (CPEs) were caused by epizootic hematopoietic necrosis virus (EHNV), Andrias davidianus iridovirus (ADIV), and Bohle iridovirus (BIV) infections in the fish cells and the viral titers (average value) of EHNV, ADIV, and BIV in GH cells reached 10(5...
January 18, 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/28083736/curcuma-longa-as-additive-in-the-diet-for-astyanax-aff-bimaculatus
#4
Pollyanna de Moraes França Ferreira, Maria Tatiana Soares Martins, Débora Werneck Caldas, Juliana Rodrigues Gomes, Jerusa Maria de Oliveira, Ana Lucia Salaro, Juliana Silva Rocha, Jener Alexandre Sampaio Zuanon
The aim of the present study was to evaluate the effect of turmeric (Curcuma longa) as additive in the diet for Astyanax aff. bimaculatus. Fish (0.83 ± 0.04 g) were fed, for 60 days, with six diets containing 0.0, 20.0, 40.0, 60.0, 80.0, and 100.0 g turmeric kg(-1) feed. There was an increasing linear effect of turmeric on the thickness of the muscular layer, and height and width of the folds of the intestine. In the liver, a quadratic effect was observed of turmeric on the percentage of hepatocyte cytoplasm and a decreasing linear effect on the percentage of sinusoid capillaries...
January 12, 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/28078494/erratum-to-effect-of-taurine-supplementation-on-hepatic-metabolism-and-alleviation-of-cadmium-toxicity-and-bioaccumulation-in-a-marine-teleost-red-sea-bream-pagrus-major
#5
Takeshi Hano, Katsutoshi Ito, Kumiko Kono, Mana Ito, Nobuyuki Ohkubo, Kazuhiko Mochida
No abstract text is available yet for this article.
January 11, 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/28074418/identification-of-low-molecular-weight-vitellogenin-1-vg1-like-proteins-as-nucleotide-excision-repair-ner-factors-in-developing-zebrafish-danio-rerio-using-a-transcription-based-dna-repair-assay
#6
Yung-Chi Shen, Todd Hsu, Li-Bin Ling, Wen-Chian You, Chia-Wei Liu
Nucleotide excision repair (NER) removes helix-distorting DNA lesions such as UV-induced pyrimidine dimers and cisplatin-induced strand crosslinking. Our earlier studies have identified low-molecular-weight proteins homologous to the 150-kDa vitellogenin 1 (Vg1) as UV-damaged DNA-binding factors expressed in developing zebrafish (Danio rerio). This present study explored if Vg1-like proteins also participated in NER in zebrafish. Immunoblot analysis of affinity-captured 12 h post-fertilization (hpf) zebrafish extract proteins showed a transient binding of a 30-kDa Vg1-like polypeptide to UV-damaged DNA...
January 10, 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/28058519/stimulation-of-glycerol-kinase-in-grass-carp-preadipocytes-by-epa
#7
Caixia Lei, Jingjing Tian, Hong Ji
This study was conducted to assess the effect of eicosapentaenoic acid (EPA) on grass carp preadipocyte glycerol kinase (GyK) expression, as well as to explore the mechanism. Here, we cloned partial sequence of grass carp GyK gene and analyzed its tissue distribution. The result showed that GyK gene expressed most in the liver, followed by adipose tissue and the kidney. Besides, 400 μM oleic acid (18:1n-9, OA) was used to establish a hypertrophic preadipocyte model. GyK gene expression and enzyme activity were significantly enhanced after model cells were treated with 100 μM eicosapentaenoic acid (20:5n-3, EPA) for 6, 12, and 24 h...
January 5, 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/28054243/differential-expression-and-mirna-regulation-of-the-gstp1-gene-in-the-regenerating-liver-of-chiloscyllium-plagiosum
#8
Yinghua Ge, Jiewen Zhang, Xinyi Shi, Conger Lu, Lingrong Yang, Yuanyuan Li, Yanna Chen, Dandan Cheng, Jing Bai, Zhengbing Lv, Lili Liu
Liver regeneration is a complicated process, and understanding the regulatory mechanism will be helpful in the treatment of diseases associated with liver. In this study, the one-third liver resection model was established in Chiloscyllium plagiosum, and the whole transcriptome of the C. plagiosum was generated using the Illumina-Solexa sequencing platform. Differentially expressed genes were analyzed using bioinformatics methods and verified using quantitative real-time PCR (qRT-PCR). Using miRanda and TargetScan, we screened the microRNA library for miRNAs that target the glutathione S-transferase P1(GSTP1) gene...
January 4, 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/28028742/growth-enzymatic-glutathione-peroxidase-activity-and-biochemical-status-of-juvenile-barramundi-lates-calcarifer-fed-dietary-fermented-soybean-meal-and-organic-selenium
#9
I Ilham, Ravi Fotedar
Solvent-extracted soybean meal (SBM) was fermented using baker's yeast Saccharomyces cerevisae at 30 °C for 5 days. Four isonitrogenous and isocaloric diets containing 75% SBM protein, either fermented or non-fermented (SBM and FSBM), and supplemented or not with organic Se (OS) (SBMOS and FSBMOS), were fed to triplicate groups of juvenile barramundi (Lates calcarifer) (initial weight of 5 g) for 75 days. A fishmeal (FM)-based diet formulated for juvenile barramundi was used as a reference diet. The growth of fish was significantly affected by either the interaction of SBM type or by the OS level...
December 27, 2016: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/28013424/effects-of-different-salinities-on-growth-performance-survival-digestive-enzyme-activity-immune-response-and-muscle-fatty-acid-composition-in-juvenile-american-shad-alosa-sapidissima
#10
Zhi-Feng Liu, Xiao-Qiang Gao, Jiu-Xiang Yu, Xiao-Ming Qian, Guo-Ping Xue, Qiao-Yun Zhang, Bao-Liang Liu, Lei Hong
The effects of salinity on survival, growth, special activity of digestive enzymes, nonspecific immune response, and muscle fatty acid composition were evaluated in the American shad (Alosa sapidissima). Juveniles of 35 days after hatching were reared at 0 (control), 7, 14, 21, and 28 ppt for 60 days. At the end of the experiment, juvenile American shad presented higher survival and specific growth rate (SGR) in salinity group (7, 14, and 21 ppt) than control group (P < 0.05). The special activity of trypsin and chymotrypsin was highest in fish reared at 21 ppt, while the highest lipase special activity was obtained in control group (P < 0...
December 24, 2016: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/28013423/cloning-expression-profiling-and-promoter-functional-analysis-of-bone-morphogenetic-protein-6-and-7-in-tongue-sole-cynoglossus-semilaevis
#11
Qian Ma, Wenrong Feng, Zhimeng Zhuang, Shufang Liu
Bone morphogenetic proteins (BMPs) play crucial roles in vertebrate developmental process and are associated with the mechanisms which drive early skeletal development. As a first approach to elucidating the role of BMPs in regulating fish bone formation and growth, we describe the cloning, expression profiling and promoter functional analysis of bmp6 and bmp7 in tongue sole (Cynoglossus semilaevis). The full length of bmp6 and bmp7 cDNA sequences is 1939 and 1836 bp, which encodes a protein of 428 and 427 amino acids, respectively...
December 24, 2016: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/28013422/acute-fluoxetine-treatment-increases-aggressiveness-in-juvenile-matrinx%C3%A3-brycon-amazonicus
#12
Carla Patricia Bejo Wolkers, Mônica Serra, Augusto Barbosa Júnior, Elisabeth Criscuolo Urbinati
Fluoxetine (FLX) is a selective serotonin (5-HT) reuptake inhibitor known for its effects modifying aggressiveness, personality traits, and anxiety-like behaviors. The aim of the present study was to evaluate the influence of the acute treatment, by immersion, with FLX on aggressive behavior of resident Brycon amazonicus fish. Fish pretreated with FLX presented an increase in aggressiveness, evidenced by the increase on the number of bites and chases against the intruder and a decrease in latency for the first attack, when compared to control fish...
December 24, 2016: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/28012026/role-of-cyclooxygenase-mediated-metabolites-in-lipid-metabolism-and-expression-of-some-immune-related-genes-in-juvenile-grass-carp-ctenopharyngodon-idellus-fed-arachidonic-acid
#13
Jing-Jing Tian, Cai-Xia Lei, Hong Ji, Ai Jin
Cyclooxygenase (COX) catalyzes the conversion of arachidonic acid (ARA) to prostaglandins, and COX-mediated metabolites play important roles in the regulation of lipid metabolism and immunity in mammals. However, such roles of COX in fish remain largely unknown. In this study, we designed three semi-purified diets, namely ARA-free (control), ARA, and ARA + acetylsalicylic acid (ASA; a COX inhibitor), and used them to feed grass carp (27.65 ± 3.05 g) for 8 weeks. The results showed that dietary ARA significantly increased the amount of ARA in the hepatopancreas, muscle, and kidney (P < 0...
December 23, 2016: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/28000079/akts-pkbs-molecular-characterization-tissue-expression-and-transcriptional-responses-to-insulin-and-or-wortmannin-in-yellow-catfish-pelteobagrus-fulvidraco
#14
Mei-Qin Zhuo, Ya-Xiong Pan, Kun Wu, Yi-Huan Xu, Li-Han Zhang, Zhi Luo
In the present study, four AKT isoforms termed AKT1, AKT2, AKT3a and AKT3b were isolated and characterized from yellow catfish. Their molecular characterizations, tissue expressions and transcriptional responses to insulin and/or wortmannin were determined. The validated complementary DNA (cDNA) of yellow catfish AKT1, AKT2, AKT3a and AKT3b were 1422, 1431, 1389 and 1440 bp in length, encoding the peptide of 472, 475, 462 and 479 amino acid residues, respectively. The amino acid sequences of yellow catfish AKTs possessed all the characteristics of AKTs in other species...
December 20, 2016: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/27957678/haemato-biochemical-non-specific-immunity-antioxidant-capacity-and-histopathological-changes-in-labeo-rohita-fingerlings-fed-rubber-protein-isolate
#15
Femi John Fawole, N P Sahu, K K Jain, S Gupta, K V Rajendran, N Shamna, Nalini Poojary
A 60-day feeding trial was conducted to evaluate the haemato-biochemical, innate immune response, antioxidant capacity and histopathological changes in Labeo rohita fingerlings fed rubber protein isolates (RPI). One hundred and eighty fingerlings (average weight 4.45 ± 0.01 g) were distributed into five experimental groups in triplicate and fed with isonitrogenous and isocaloric diets. Soybean protein isolate (SPI) served as the reference diet (Control), and the treatment diets were formulated as RPI25, RPI50, RPI75 and RPI100 replacing 25, 50, 75 and 100% of SPI protein, respectively...
December 12, 2016: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/27942900/developmental-changes-in-digestive-enzyme-activity-in-american-shad-alosa-sapidissima-during-early-ontogeny
#16
Xiao-Qiang Gao, Zhi-Feng Liu, Chang-Tao Guan, Bin Huang, Ji-Lin Lei, Juan Li, Zheng-Long Guo, Yao-Hui Wang, Lei Hong
In order to assess the digestive physiological capacity of the American shad Alosa sapidissima and to establish feeding protocols that match larval nutritional requirements, we investigated the ontogenesis of digestive enzymes (trypsin, amylase, lipase, pepsin, alkaline phosphatase, and leucine aminopeptidase) in larvae, from hatching to 45 days after hatching (DAH). We found that all of the target enzymes were present at hatching, except pepsin, which indicated an initial ability to digest nutrients and precocious digestive system development...
December 9, 2016: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/27909949/organ-specific-effects-of-low-dose-zinc-pre-exposure-on-high-dose-zinc-induced-mitochondrial-dysfunction-in-large-yellow-croaker-pseudosciaena-crocea
#17
Jia-Lang Zheng, Shuang-Shuang Yuan, Bin Shen, Chang-Wen Wu
The study was carried out to evaluate the effects of low-dose zinc (Zn) pre-exposure on survival rate, new Zn accumulation, and mitochondrial bioenergetics in the liver and spleen of large yellow croaker exposed to high-dose Zn. To the end, fish were pre-exposed to 0 and 2 mg L(-1) Zn for 48 h and post-exposed to 0 and 12 mg L(-1) Zn for 48 h. Twelve milligrams Zn per liter exposure alone reduced survival rate, but the effect did not appear in the 2 mg L(-1) Zn pre-exposure groups. Two milligrams per liter Zn pre-exposure also ameliorated 12 mg Zn L(-1) induced new Zn accumulation, reactive oxygen species (ROS) levels, and mitochondrial swelling in the liver...
December 1, 2016: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/27889848/changes-of-ammonia-urea-contents-and-transaminase-activity-in-the-body-during-aerial-exposure-and-ammonia-loading-in-chinese-loach-paramisgurnus-dabryanus
#18
Yun-Long Zhang, Hai-Long Zhang, Ling-Yu Wang, Bei-Yi Gu, Qi-Xue Fan
The Paramisgurnus dabryanus was exposed to 30 mmol L(-1) NH4Cl solution and air to assessing the change of body ammonia and urea contents and the activities of alanine aminotransferase (ALT) and aspartate transaminase (AST). After 48 h of ammonia exposure, ammonia concentration in the plasma, brain, liver and muscle were 3.3-fold, 5.6-fold, 3.5-fold and 4.2-fold, respectively, those of the control values. Plasma, brain, liver and muscle ammonia concentrations increased to 2.2-fold, 3.3-fold, 2.5-fold and 2...
November 26, 2016: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/27882445/enhanced-dietary-formulation-to-mitigate-winter-thermal-stress-in-gilthead-sea-bream-sparus-aurata-a-2d-dige-plasma-proteome-study
#19
Denise Schrama, Nadège Richard, Tomé S Silva, Filipe A Figueiredo, Luís E C Conceição, Richard Burchmore, David Eckersall, Pedro M L Rodrigues
Low water temperatures during winter are common in farming of gilthead sea bream in the Mediterranean. This causes metabolic disorders that in extreme cases can lead to a syndrome called "winter disease." An improved immunostimulatory nutritional status might mitigate the effects of this thermal metabolic stress. A trial was set up to assess the effects of two different diets on gilthead sea bream physiology and nutritional state through plasma proteome and metabolites. Four groups of 25 adult gilthead sea bream were reared during winter months, being fed either with a control diet (CTRL) or with a diet called "winter feed" (WF)...
November 24, 2016: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/27864748/irs1-and-irs2-molecular-characterization-tissue-expression-and-transcriptional-regulation-by-insulin-in-yellow-catfish-pelteobagrus-fulvidraco
#20
Mei-Qin Zhuo, Ya-Xiong Pan, Kun Wu, Yi-Huan Xu, Li-Han Zhang, Zhi Luo
The insulin receptor substrate (IRS) proteins, in particular, IRS1 and IRS2, are the key downstream players of insulin signaling pathway and the regulation of lipid metabolism. In the present study, two genes of IRS (IRS1 and IRS2) were isolated and characterized from yellow catfish Pelteobagrus fulvidraco. Their molecular characterizations, tissue expressions, and transcriptional levels by insulin both in vivo and in vitro were determined. The validated complementary DNAs encoding for IRS1 and IRS2 were 3693 and 3177 bp in length, encoding proteins of 1230 and 1058 amino acid residues, respectively...
November 18, 2016: Fish Physiology and Biochemistry
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