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Bioelectrochemistry

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https://www.readbyqxmd.com/read/28236756/electrical-activity-of-cellobiose-dehydrogenase-adsorbed-on-thiols-influence-of-charge-and-hydrophobicity
#1
P Lamberg, J Hamit-Eminovski, M D Toscano, O Eicher-Lorka, G Niaura, T Arnebrant, S Shleev, T Ruzgas
The interface between protein and material surface is of great research interest in applications varying from implants, tissue engineering to bioelectronics. Maintaining functionality of bioelements depends greatly on the immobilization process. In the present study direct electron transfer of cellobiose dehydrogenase from Humicola insolens (HiCDH), adsorbed on four different self-assembled monolayers (SAMs) formed by 5-6 chain length carbon thiols varying in terminal group structure was investigated. By using a combination of quartz crystal microbalance with dissipation, ellipsometry and electrochemistry the formation and function of the HiCDH film was studied...
February 16, 2017: Bioelectrochemistry
https://www.readbyqxmd.com/read/28236755/caspase-dependent-apoptosis-induced-in-yeast-cells-by-nanosecond-pulsed-electric-fields
#2
Povilas Simonis, Skirmantas Kersulis, Voitech Stankevich, Vytautas Kaseta, Egle Lastauskiene, Arunas Stirke
Saccharomyces cerevisiae yeast cells were used as a model organism to investigate the effects of various pulsed electric fields on the programed death of such cells. These were exposed to electric field pulses with field strengths (E) of up to 220kV/cm. The effects of square shaped pulses having different durations (τ=10-90ns) and different pulse numbers (pn=1-5) were then analysed. The obtained results show that nanosecond pulses can induce the death of such cells, which in turn is dependent on the electric field pulse parameters and increase with the rise in E, τ and pn...
February 6, 2017: Bioelectrochemistry
https://www.readbyqxmd.com/read/28237705/impedance-spectroscopy-as-an-indicator-for-successful-in-vivo-electric-field-mediated-gene-delivery-in-a-murine-model
#3
Reginald M Atkins, Timothy J Fawcett, Richard Gilbert, Andrew M Hoff, Richard Connolly, Douglas W Brown, Anthony J Llewellyn, Mark J Jaroszeski
In vivo gene electro transfer technology has been very successful both in animal models and in clinical trials over the past 20years. However, variable transfection efficiencies can produce inconsistent outcomes. This can be due to differences in tissue architecture and/or chemical composition which may effectively create unique biological environments from subject to subject that may respond differently to the identical electric pulses. This study investigates the integration of impedance spectroscopy into the gene electro transfer process to measure murine skin impedance spectra before, during (after pulse delivery), and after gene electro transfer pulse application to determine if changes in impedance correlate with reporter gene expression...
January 27, 2017: Bioelectrochemistry
https://www.readbyqxmd.com/read/28126645/study-of-overall-and-local-electrochemical-responses-of-oxide-films-grown-on-cocr-alloy-under-biological-environments
#4
I Diaz, J F Martinez-Lerma, R Montoya, I Llorente, M L Escudero, M C García-Alonso
The interaction of the physiological medium and living tissues with the implant surfaces in biological environments is regulated by biopotentials that induce changes in the chemical composition, structure and thickness of the oxide film. In this work, oxide films grown on CoCr alloys at 0.5 V vs Ag/AgCl and 0.7 V vs Ag/AgCl have been characterized through overall and localized electrochemical techniques in a phosphate buffer solution and 0.3% hyaluronic acid. Nanopores of 10-50nm diameter are homogeneously distributed along the surface in the oxide film formed at 0...
January 17, 2017: Bioelectrochemistry
https://www.readbyqxmd.com/read/28167385/spatiometabolic-stratification-of-anoxic-biofilm-in-prototype-bioelectrogenic-system
#5
G Mohanakrishna, Sai Kishore Butti, R Kannaiah Goud, S Venkata Mohan
A prototype bio-catalyzed electrogenic system integrated with a biological treatment process (SBR-BET) was evaluated to study specific function of anoxic condition on the electrogenic activity. A multiphasic approach was employed, where the influence of DO on bio-electrogenic activity was optimized initially, later optimal anode to cathode inter-electrode distance was enumerated. Amongst the four electrode distances evaluated, 2cm showed higher power output. Bioelectrokinetics analysis was used to validate the system performance with the experimental variation studied...
January 9, 2017: Bioelectrochemistry
https://www.readbyqxmd.com/read/28063751/mechanisms-of-inactivation-of-candida-humilis-and-saccharomyces-cerevisiae-by-pulsed-electric-fields
#6
Qi-Xing Ou, Marija Nikolic-Jaric, Michael Gänzle
AIMS: This study aimed to determine how electric field strength, pulse width and shape, and specific energy input relate to the effect of pulsed electric fields (PEF) on viability and membrane permeabilization in Candida humilis and Saccharomyces cerevisiae suspended in potassium phosphate buffer. METHODS AND RESULTS: Cells were treated with a micro-scale system with parallel plate electrodes. Propidium iodide was added before or after treatments to differentiate between reversible and irreversible membrane permeabilization...
December 30, 2016: Bioelectrochemistry
https://www.readbyqxmd.com/read/28081473/cortisol-extraction-through-human-skin-by-reverse-iontophoresis
#7
Stephanie A Ventura, Jason Heikenfeld, Tiffany Brooks, Leyla Esfandiari, Steven Boyce, Yoonjee Park, Gerald B Kasting
Continuous monitoring of cortisol at the surface of the skin would advance the diagnosis and treatment of cortisol-related diseases, or of elevated cortisol levels related to stress in otherwise healthy populations. Reliable and accurate detection of cortisol at the skin surface remains a limiting factor in real-time monitoring of cortisol. To address this limitation, cortisol extraction through excised human skin by reverse iontophoresis was studied in vitro in side-by-side diffusion cells using a radiolabeled probe...
April 2017: Bioelectrochemistry
https://www.readbyqxmd.com/read/28064043/conductive-hydrogel-composed-of-1-3-5-benzenetricarboxylic-acid-and-fe-3-used-as-enhanced-electrochemical-immunosensing-substrate-for-tumor-biomarker
#8
Huiqiang Wang, Hongliang Han, Zhanfang Ma
In this work, a new conductive hydrogel was prepared by a simple cross-linking coordination method using 1,3,5-benzenetricarboxylic acid as the ligand and Fe(3+) as the metal ion. The hydrogel film was formed on a glassy carbon electrode (GCE) by a drop coating method, which can dramatically facilitate the transport of electrons. A sensitive label-free electrochemical immunosensor was fabricated following electrodeposition of gold nanoparticles (AuNPs) on a hydrogel film and immobilization of an antibody. Neuron-specific enolase (NSE), a lung cancer biomarker, was used as the model analyte to be detected...
April 2017: Bioelectrochemistry
https://www.readbyqxmd.com/read/28063413/chronopotentiometric-sensing-of-specific-interactions-between-lysozyme-and-the-dna-aptamer
#9
Veronika Ostatná, Veronika Kasalová-Vargová, László Kékedy-Nagy, Hana Černocká, Elena E Ferapontova
Specific DNA-protein interactions are vital for cellular life maintenance processes, such as transcriptional regulation, chromosome maintenance, replication and DNA repair, and their monitoring gives valuable information on molecular-level organization of those processes. Here, we propose a new method of label-free electrochemical sensing of sequence specific binding between the lysozyme protein and a single stranded DNA aptamer specific for lysozyme (DNAapta) that exploits the constant current chronopotentiometric stripping (CPS) analysis at modified mercury electrodes...
April 2017: Bioelectrochemistry
https://www.readbyqxmd.com/read/28006672/gene-electrotransfer-into-skin-using-noninvasive-multi-electrode-array-for-vaccination-and-wound-healing
#10
Spela Kos, Kevin Vanvarenberg, Tanja Dolinsek, Maja Cemazar, Jure Jelenc, Véronique Préat, Gregor Sersa, Gaëlle Vandermeulen
Skin is an attractive target for gene electrotransfer due to its easy accessibility and its interesting immune properties. Since electrodes are often invasive and frequently induce discomfort during pulse application, there is a fundamental need for non-invasive electrodes for skin delivery. We developed circular pin non-invasive multi-electrode array (MEA), suitable for different clinical applications. MEA was first employed to deliver a luciferase reporter gene. Then, it was used to deliver a DNA vaccine coding for ovalbumin or a plasmid encoding hCAP-18/LL-37 for promoting wound healing...
April 2017: Bioelectrochemistry
https://www.readbyqxmd.com/read/27992855/amplified-detection-of-leukemia-cancer-cells-using-an-aptamer-conjugated-gold-coated-magnetic-nanoparticles-on-a-nitrogen-doped-graphene-modified-electrode
#11
Seyyed Mehdi Khoshfetrat, Masoud A Mehrgardi
The increasing demands for early, accurate and ultrasensitive diagnosis of cancers demonstrate the importance of the development of new amplification strategies or diagnostic technologies. In the present study, an aptamer-based electrochemical biosensor for ultrasensitive and selective detection of leukemia cancer cells has been introduced. The thiolated sgc8c aptamer was immobilized on gold nanoparticles-coated magnetic Fe3O4 nanoparticles (Apt-GMNPs). Ethidium bromide (EB), intercalated into the stem of the aptamer hairpin, provides the read-out signal for the quantification of the leukemia cancer cells...
April 2017: Bioelectrochemistry
https://www.readbyqxmd.com/read/27855361/alzheimer-s-disease-amyloid-beta-peptides-in-vitro-electrochemical-oxidation
#12
Teodor Adrian Enache, Ana Maria Oliveira-Brett
The oxidative behaviour of the human amyloid beta (Aβ1-40 and Aβ1-42) peptides and a group of similar peptides: control inverse (Aβ40-1 and Aβ42-1), mutants (Aβ1-40Phe(10) and Aβ1-40Nle(35)), rat Aβ1-40Rat, and fragments (Aβ1-28, Aβ1-16, Aβ10-20, Aβ12-28, and Aβ17-42), in solution or adsorbed, at a glassy carbon electrode, by cyclic and differential pulse voltammetry, were investigated and compared. Structurally the Aβ1-40 and Aβ1-42 sequences contain five electroactive amino acid residues, one tyrosine (Tyr(10)), three histidines (His(6), His(13) and His(14)) and one methionine (Met(35))...
April 2017: Bioelectrochemistry
https://www.readbyqxmd.com/read/27837683/molecular-design-of-cytocompatible-amphiphilic-redox-active-polymers-for-efficient-extracellular-electron-transfer
#13
Masahiro Kaneko, Masahito Ishikawa, Kazuhito Hashimoto, Shuji Nakanishi
For electrochemical regulations of the intracellular metabolisms, lipophilic electron mediators with cell membrane permeability have been conventionally used. We have recently developed amphiphilic, cell-membrane permeable polymer composed of hydrophilic 2-methacryloyloxyethyl phosphorylcholine and hydrophobic redox-active units as a new category of electron mediator. The advantage of the redox active polymer is that we can obtain appropriate molecules in a synthetic bottom-up manner. Here we report that the rate of the extracellular electron transfer (EET) through the redox active polymer can be regulated by sophisticated molecular design of the polymers...
April 2017: Bioelectrochemistry
https://www.readbyqxmd.com/read/27835761/correlation-between-circuital-current-cu-ii-reduction-and-cellular-electron-transfer-in-eab-isolated-from-cu-ii-reduced-biocathodes-of-microbial-fuel-cells
#14
Jingya Shen, Liping Huang, Peng Zhou, Xie Quan, Gianluca Li Puma
The performance of four indigenous electrochemically active bacteria (EAB) (Stenotrophomonas maltophilia JY1, Citrobacter sp. JY3, Pseudomonas aeruginosa JY5 and Stenotrophomonas sp. JY6) was evaluated for Cu(II) reduction on the cathodes of microbial fuel cells (MFCs). These EAB were isolated from well adapted mixed cultures on the MFC cathodes operated for Cu(II) reduction. The relationship between circuital current, Cu(II) reduction rate, and cellular electron transfer processes was investigated from a mechanistic point of view using X-ray photoelectron spectroscopy, scanning electronic microscopy coupled with energy dispersive X-ray spectrometry, linear sweep voltammetry and cyclic voltammetry...
April 2017: Bioelectrochemistry
https://www.readbyqxmd.com/read/27816024/characterization-and-performance-of-anodic-mixed-culture-biofilms-in-submersed-microbial-fuel-cells
#15
Beenish Saba, Ann D Christy, Zhongtang Yu, Anne C Co, Rafiq Islam, Olli H Tuovinen
Microbial fuel cells (MFCs) were designed for laboratory scale experiments to study electroactive biofilms in anodic chambers. Anodic biofilms and current generation during biofilm growth were examined using single chambered MFCs submersed in algal catholyte. A culture of the marine green alga Nanochloropsis salina was used as a biocatholyte, and a rumen fluid microbiota was the anodic chamber inoculum. Electrical impedance spectroscopy was performed under varying external resistance once a week to identify mass transport limitations at the biofilm-electrolyte interface during the four-week experiment...
February 2017: Bioelectrochemistry
https://www.readbyqxmd.com/read/27768936/calcium-induced-calmodulin-conformational-change-electrochemical-evaluation
#16
Isabel P G Fernandes, Ana Maria Oliveira-Brett
Calmodulin (CaM) is an essential protein present in all eukaryote cells, ranging from vertebrates to unicellular organisms. CaM is the most important Ca(2+) signalling protein, composed of two domains, N- and C-terminal domains, linked by a flexible central α-helix, and is responsible for the regulation of numerous calcium-mediated signalling pathways. Four calcium ions bind to CaM, changing its conformation and determining how it recognizes and regulates its cellular targets. The oxidation mechanism of native and denatured CaM, at a glassy carbon electrode, was investigated using differential pulse voltammetry and electrochemical impedance spectroscopy...
February 2017: Bioelectrochemistry
https://www.readbyqxmd.com/read/27756010/electrotonic-potentials-in-aloe-vera-l-effects-of-intercellular-and-external-electrodes-arrangement
#17
Alexander G Volkov, Eunice K Nyasani, Clayton Tuckett, Jessenia M Scott, Mariah M Z Jackson, Esther A Greeman, Ariane S Greenidge, Devin O Cohen, Maia I Volkova, Yuri B Shtessel
Electrostimulation of plants can induce plant movements, activation of ion channels, ion transport, gene expression, enzymatic systems activation, electrical signaling, plant-cell damage, enhanced wound healing, and influence plant growth. Here we found that electrical networks in plant tissues have electrical differentiators. The amplitude of electrical responses decreases along a leaf and increases by decreasing the distance between polarizing Pt-electrodes. Intercellular Ag/AgCl electrodes inserted in a leaf and extracellular Ag/AgCl electrodes attached to the leaf surface were used to detect the electrotonic potential propagation along a leaf of Aloe vera...
February 2017: Bioelectrochemistry
https://www.readbyqxmd.com/read/27732908/when-and-how-the-melittin-ion-channel-exhibits-ohmic-behavior
#18
Lucia Becucci, Giovanni Aloisi, Rolando Guidelli
Melittin exhibits an ohmic behavior in a lipid bilayer having a DOPC distal leaflet and interposed between a 2.25nm tetraethyleneoxy chain tethered to a mercury drop and an aqueous solution. This behavior is induced in a pH6.8 buffer solution of 0.8μg/mL melittin by a pretreatment consisting of series of electrochemical impedance spectroscopy measurements at bias potentials varied by 50mV steps over a transmembrane potential range from about +250 to -250mV. This metastable ohmic behavior remains unchanged for hours, even after acidifying the solution to pH3...
February 2017: Bioelectrochemistry
https://www.readbyqxmd.com/read/27693939/irreversible-electroporation-inhibits-pro-cancer-inflammatory-signaling-in-triple-negative-breast-cancer-cells
#19
Ishan Goswami, Sheryl Coutermarsh-Ott, Ryan G Morrison, Irving C Allen, Rafael V Davalos, Scott S Verbridge, Lissett R Bickford
Low-level electric fields have been demonstrated to induce spatial re-distribution of cell membrane receptors when applied for minutes or hours. However, there is limited literature on the influence on cell signaling with short transient high-amplitude pulses typically used in irreversible electroporation (IRE) for cancer treatment. Moreover, literature on signaling pertaining to immune cell trafficking after IRE is conflicting. We hypothesized that pulse parameters (field strength and exposure time) influence cell signaling and subsequently impact immune-cell trafficking...
February 2017: Bioelectrochemistry
https://www.readbyqxmd.com/read/27676191/radiosensitization-of-oral-tongue-squamous-cell-carcinoma-by-nanosecond-pulsed-electric-fields-nspefs
#20
Jinsong Guo, Yu Wang, Jing Wang, Jue Zhang, Jing Fang
Nanosecond pulsed electric fields (nsPEFs) are a non-thermal and non-toxic technology that induce a myriad of biological effects. They have been proven to be effective in tumor shrinkage, but few studies focus on its radiosensitization in oral tongue squamous cell carcinoma. The purpose of this research was to study the radiosensitization effect of nsPEFs on a human oral tongue cancer cell line Tca8113 and to investigate the potential antitumor mechanism. A Tca8113 cell line was tested respectively by MTT assay, clonogenic assay, flow cytometry assay, annexin V-FITC/PI assay, mitochondrial potential assay and total nitric oxide assay...
February 2017: Bioelectrochemistry
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