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Shiue-Lin Li, Ming-Der Bai, Chia-Jung Hsiao, Sheng-Shung Cheng, Kenneth H Nealson
A mediated glassy carbon electrode covered by a thin-film polyviologen was used in the present study to rapidly detect bioactivity in a mixed-culture chemostat (dominated by Clostridium sp.). With the addition of 1mM hexacyanoferrate and 9mM glucose, the current increasing rate (dI/dt) measured under a poised potential of 500mV (vs. Ag/AgCl) can be defined as the quantity of metabolic activity. In the experiment of restoring the chemostat from stop-feeding, it is suggested that when the dI/dt was >2μAmin(-1), the influent pump could be directly turned on to maintain the high dilution rate of 0...
August 9, 2017: Bioelectrochemistry
Claudia Rojas, Ignacio T Vargas, Mary Ann Bruns, John M Regan
The limited database of acidophilic or acidotolerant electrochemically active microorganisms prevents advancements on microbial fuel cells (MFCs) operated under low pH. In this study, three MFCs were used to enrich cathodic biofilms using acid mine drainage (AMD) sediments as inoculum. Linear sweep voltammetry showed cathodic current plateaus of 5.5 (±0.7) mA at about -170mV vs Ag/AgCl and 8.5 (±0.9) mA between -500mV to -450mV vs Ag/AgCl for biofilms developed on small graphite fiber brushes. After gamma irradiation, biocathodes exhibited a decrease in current density approaching that of abiotic controls...
August 3, 2017: Bioelectrochemistry
Stéphane Pinck, Mathieu Etienne, Manuel Dossot, Frédéric P A Jorand
A living material was formed by self-assembly of bacterial cells (Shewanella oneidensis MR-1 or Pseudomonas fluorescens) with carbon nanotubes in the presence of cytochrome c from a bovine heart with the goal to mimic electroactive biofilms. The role of cytochrome c on self-assembly, cell viability and extracellular electron transfer was studied. Scanning electron microscopy and dynamic light scattering experiments highlighted its role on the self-assembly of bacteria‑carbon nanotube aggregates within only 2h in solution...
August 1, 2017: Bioelectrochemistry
Kasra Saeedfar, Lee Yook Heng, Chew Poh Chiang
Multi-wall carbon nanotubes (MWCNTs) were modified to design a new DNA biosensor. Functionalized MWCNTs were equipped with gold nanoparticles (GNPs) (~15nm) (GNP-MWCNTCOOH) to construct DNA biosensors based on carbon-paste screen-printed (SPE) electrodes. GNP attachment onto functionalized MWCNTs was carried out by microwave irradiation and was confirmed by spectroscopic studies and surface analysis. DNA biosensors based on differential pulse voltammetry (DPV) were constructed by immobilizing thiolated single-stranded DNA probes onto GNP-MWCNTCOOH...
July 31, 2017: Bioelectrochemistry
Alexander G Volkov, Kunning G Xu, Vladimir I Kolobov
Low temperature (cold) plasma finds an increasing number of applications in biology, medicine and agriculture. In this paper, we report a new effect of plasma induced morphing and movements of Venus flytrap and Mimosa pudica. We have experimentally observed plasma activation of sensitive plant movements and morphing structures in these plants similar to stimulation of their mechanosensors in vivo. Application of an atmospheric pressure argon plasma jet to the inside or outside of a lobe, midrib, or cilia in Dionaea muscipula Ellis induces trap closing...
July 29, 2017: Bioelectrochemistry
Claudia M Martinez, Xiuping Zhu, Bruce E Logan
The application of immobilized redox mediators (RMs) in microbial fuel cells (MFCs) is an emerging technology for electricity generation with simultaneous azo dye decolorization due to facilitated electrons transfer from bacteria to anodes and azo dyes. The use of immobilized RMs avoids the requirement of their continuous dosing in MFCs, which has been the main limitation for practical applications. Two strategies of anthraquinones-2,6-disulphonic salt (AQDS) immobilization, AQDS immobilized with polyvinyl alcohol particles and AQDS immobilized on anodes by electropolymerization, were evaluated and compared to achieve simultaneous reactive red 2 (RR2) dye reduction and bioelectricity generation...
July 26, 2017: Bioelectrochemistry
Yogeswaran Umasankar, Bal-Ram Adhikari, Aicheng Chen
An efficient approach for immobilizing alcohol dehydrogenase (ADH) while enhancing its electron transfer ability has been developed using poly(2-(trimethylamino)ethyl methacrylate) (MADQUAT) cationic polymer and carbon nanoscaffolds. The carbon nanoscaffolds were comprised of single-walled carbon nanotubes (SWCNTs) wrapped with reduced graphene oxide (rGO). The ADH entrapped within the MADQUAT that was present on the carbon nanoscaffolds exhibited a high electron exchange capability with the electrode through its cofactor β-nicotinamide adenine dinucleotide hydrate and β-nicotinamide adenine dinucleotide reduced disodium salt hydrate (NAD(+)/NADH) redox reaction...
July 26, 2017: Bioelectrochemistry
Christin Koch, Anne Kuchenbuch, Frauke Kracke, Paul V Bernhardt, Jens Krömer, Falk Harnisch
Microbial electrosynthesis is a highly promising application of microbial electrochemical technologies for the sustainable production of organic compounds. At the same time a multitude of questions need to be answered and challenges to be met. Central for its further development is using appropriate electroactive microorganisms and their efficient extracellular electron transfer (EET) as well as wiring of the metabolism to EET. Among others, Clostridia are believed to represent electroactive microbes being highly promising for microbial electrosynthesis...
July 25, 2017: Bioelectrochemistry
Beibei Wang, Xueping Ji, Jujie Ren, Ruixing Ni, Lin Wang
A simple, ultrasensitive peroxynitrite anion (ONOO(-)) electrochemical sensing platform was developed by immobilizing hemin on a density controllable electrochemically reduced graphene oxide-Au nanoparticles (ERGO-AuNPs) nanohybrids. The ERGO-AuNPs in situ nanohybrids were produced onto a glass carbon electrode (GCE) by one-step electrodeposition, the density of which could be easily controlled by electrodeposited time. The morphology of ERGO-AuNPs nanohybrids was characterized by a scanning electron microscope (SEM)...
July 15, 2017: Bioelectrochemistry
Ayman Ali Saeed, Josep Lluís Acero Sánchez, Ciara K O'Sullivan, Mohammed Nooredeen Abbas
Two different DNA (ERBB2c and CD24c) modified gold nanoparticles and graphene oxide loaded on glassy carbon electrodes were prepared for early detection of breast cancer markers by electrochemical detection of HER2. Comparative study of ERBB2c and CD24c for the detection was carried out. A "sandwich-type" detection strategy was employed in this electrochemical DNA biosensor and its response was measured by amperometric detection. The electrochemical signal enhancement achieved via gold nanoparticles and grapheme oxide system allowed for sensitive detection of the breast cancer biomarker ERBB2 and the control marker CD24...
July 14, 2017: Bioelectrochemistry
Hong-Qi Xia, Yuki Kitazumi, Osamu Shirai, Hiroki Ozawa, Maki Onizuka, Takuji Komukai, Kenji Kano
The effects of three types of water-soluble carbon nanotubes (CNTs) of different lengths on the direct electron transfer (DET)-type bioelectrocatalysis of redox enzymes were investigated. Bilirubin oxidase (BOD), copper efflux oxidase (CueO), and a membrane-bound NiFe hydrogenase (H2ase) were used as model redox enzymes for four-electron dioxygen (O2) reduction (in the case of BOD and CueO) and two-electron dihydrogen (H2) oxidation (in the case of H2ase). As a result, diffusion-controlled O2 reduction in an O2-saturated neutral buffer was realized by BOD on CNTs of a length of 1μm, but the catalytic current densities decreased as the length of CNTs increased...
July 13, 2017: Bioelectrochemistry
Guillermo Pozo, Yang Lu, Sebastien Pongy, Jürg Keller, Pablo Ledezma, Stefano Freguia
Selective microbial retention is of paramount importance for the long-term performance of cathodic sulfate reduction in microbial electrolysis cells (MECs) due to the slow growth rate of autotrophic sulfate-reducing bacteria. In this work, we investigate the biofilm retention and current-to-sulfide conversion efficiency using carbon granules (CG) or multi-wall carbon nanotubes deposited on reticulated vitreous carbon (MWCNT-RVC) as electrode materials. For ~2months, the MECs were operated at sulfate loading rates of 21 to 309gSO4 -S/m(2)/d...
July 12, 2017: Bioelectrochemistry
R Rapini, G Marrazza
The growing number of contaminants requires the development of new analytical tools to meet the increasing demand for legislative actions on food safety and environmental pollution control. In this context, electrochemical aptamer-based sensors appear promising among all biosensors because they permit multiplexed analysis and provide fast response, sensitivity, specificity and low cost. The aim of this review is to give the readers an overview of recent important achievements in the development of electrochemical aptamer-based biosensors for contaminant detection over the last two years...
July 12, 2017: Bioelectrochemistry
Yi Kung, Alexey Lihachev, Saulius Šatkauskas, Keng-Li Lan, Wen-Shiang Chen
The efficacy of genomic medicine depends on gene transfer efficiency. In this area, electroporation has been found to be a highly promising method for physical gene transfer. However, electroporation raises issues related to electrical safety, tissue damage, and the number of required wounds. Concentric-type needle electrodes seek to address these issues by using a lower bias (10V), a single wound, fewer processing steps, and a smaller working area (≈10mm(3)), thus offering greater accuracy and precision...
July 8, 2017: Bioelectrochemistry
Sagarika Deepthy Tallapragada, Keya Layek, Runu Mukherjee, Kalyan Kumar Mistry, Monidipa Ghosh
In this study, an immunosensor based on screen-printed electrode (SPE) has been developed for the detection of Human Epidermal Growth Factor Receptor-2 (HER2) antigen. The SPEs were fabricated and a sandwich enzyme linked immunosorbent assay (ELISA) format was followed for the construction of the immunosensor. The capture antibody (mouse anti-human ErbB2) was coated onto the electrode surface without any prior surface modification, followed by the addition of recombinant human HER2 antigen. Biotinylated goat anti-human ErbB2 was used as the detection antibody which was linked to streptavidin conjugated horseradish peroxidase (HRP)...
June 30, 2017: Bioelectrochemistry
D Zouraris, A Zerva, E Topakas, A Karantonis
The enzyme MtPerII is a new peroxidase which has been isolated only recently from fungus Myceliophthora thermophila and has significant thermostability and stability at high H2O2 concentrations. In the present work, an electrochemical kinetic study, based on cyclic voltammetry, is performed for the first time for the catalytic decomposition of H2O2 by MtPerII, at 18°C. Leuco methylene blue (LMB) is used as a mediator and the catalytic and Michaelis constants are determined, assuming a Michaelis-Menten mechanism...
June 30, 2017: Bioelectrochemistry
N Soda, O A Arotiba
A novel polyamidoamine dendrimer-streptavidin supramolecular architecture suitable as a versatile platform for biosensor development is reported. The dendrimer was electrodeposited on a glassy carbon electrode via cyclic voltammetry. The dendrimer electrode was further modified with streptavidin by electrostatic attraction upon drop coating. The platform i.e. the dendrimer-streptavidin modified electrode was electrochemically interrogated in phosphate buffer, ferrocyanide and H2O2. The dendrimer-streptavidin platform was used in the preparation of a simple DNA biosensor as a proof of concept...
June 30, 2017: Bioelectrochemistry
Ru Jia, Dongqing Yang, Dake Xu, Tingyue Gu
Electron transfer is a rate-limiting step in microbiologically influenced corrosion (MIC) caused by microbes that utilize extracellular electrons. Cross-cell wall electron transfer is necessary to transport the electrons released from extracellular iron oxidation into the cytoplasm of cells. Electron transfer mediators were found to accelerate the MIC caused by sulfate reducing bacteria. However, there is no publication in the literature showing the effect of electron transfer mediators on MIC caused by nitrate reducing bacteria (NRB)...
June 29, 2017: Bioelectrochemistry
Mahdi Shahmohammadi Aghbolagh, Mohammad Yaser Khani Meynaq, Kenichi Shimizu, Britta Lindholm-Sethson
A supported liquid crystalline cubic phase housing glucose oxidase on an electrode surface has been suggested as bio-anode in a biofuel. The purpose of this investigation is to clarify some aspect on the mediated enzymatic oxidation of glucose in such a bio-anode where the mediator ferrocene-carboxylic acid and glucose were dissolved in the solution. The enzyme glucose oxidase was housed in the water channels of the mono-olein cubic phase. The system was investigated with cyclic voltammetry at different scan rates and the temperature was varied between 15°C and 30°C...
June 22, 2017: Bioelectrochemistry
Nancy Velasco-Alvarez, Mariano Gutiérrez-Rojas, Ignacio González
The effects of electric current on membranes associated with metabolism modifications in Aspergillus brasiliensis (niger) ATCC 9642 were studied. A 450-mL electrochemical cell with titanium ruthenium-oxide coated electrodes and packed with 15g of perlite, as inert support, was inoculated with A. brasiliensis spores and incubated in a solid inert-substrate culture (12 d; 30°C). Then, 4.5days after starting the culture, a current of 0.42mAcm(-2) was applied for 24h. The application of low-intensity electric current increased the molecular oxygen consumption rate in the mitochondrial respiratory chain, resulting in high concentrations of reactive oxygen species, promoting high lipoperoxidation levels, according to measured malondialdehyde, and consequent alterations in membrane permeability explained the high n-hexadecane (HXD) degradation rates observed here (4...
June 19, 2017: Bioelectrochemistry
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