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Shuai Liang, Baogang Zhang, Jiaxin Shi, Ting Wang, Lifeng Zhang, Zhijun Wang, Cuibai Chen
Electrochemical decolorization is of particular importance for the efficient treatment of dye wastewater. A promising electrochemical system powered by microbial fuel cells (MFCs) and intensified by Fe-C micro-electrolysis is proposed and enhanced decolorization of methyl orange (MO) is realized in this study. The decolorization efficiency reached as high as 97.1 ± 1.8% after 180 min of operation with initial MO concentration of 50 mg/L and applied voltage of 700 mV. Decolorization efficiency initially increased and then decreased with rising Fe-to-C ratio...
July 10, 2018: Bioelectrochemistry
Anna A Bulysheva, Nina Burcus, Cathryn G Lundberg, Michael P Francis, Richard Heller
Atherosclerosis induced myocardial infarction (MI) continues to be a major public health concern. Regenerative therapies that restore cardiac muscle cells are largely absent. The rate of cardiomyogenesis in adults is insufficient to compensate for MI damage. In this study, we explored the capacity of a gene therapy approach to promote cardiomyogenesis. We hypothesized that VEGF-B, critical during fetal heart development, could promote cardiomyogenesis in adult ischemic hearts. Gene electrotransfer (GET), a physical method of in vivo gene delivery, was adapted to the rat model of MI...
July 10, 2018: Bioelectrochemistry
Qiang Yuan, Y Huang, Debao Liu, Minfang Chen
Biodegradable beta-tricalcium phosphate (β-TCP) particle reinforced magnesium metal matrix composites (Mg-MMC) have attracted increasing interest for application as implant materials. This investigation was conducted to study the effect of cooling rate on the microstructure and corrosion behavior of a biodegradable β-TCP/Mg-Zn-Ca composite. The composite was fabricated under a series of cooling rates using a wedge-shaped casting mold. The microstructure of the composite was examined by optical and scanning electron microscopy, and the corrosion behavior was investigated using an electrochemical workstation and immersion tests in a simulated body fluid (SBF)...
July 7, 2018: Bioelectrochemistry
Shiue-Lin Li, Jui-Hung Yen, Kenji Kano, Shiu-Mei Liu, Chien-Liang Liu, Sheng-Shung Cheng, Hsun-Yi Chen
Using an electrochemical cell equipped with carbon felt electrodes (poised at +0.63 V vs. SHE), the current production capabilities of two Shewanella strains-NTOU1 and KR-12-were examined under various conditions with lactate as an electron donor. The metabolic charge produced in the tricarboxylic acid cycle (QTCA ) was calculated by mass-balance. The data showed a linear relation between the electric coulomb production (QEL ) and QTCA with an R2 of 0.65. In addition, a large amount of pyruvate accumulation was observed at pH = 6, rendering QTCA negative...
July 6, 2018: Bioelectrochemistry
Rosanna Ciriello, Francesca De Gennaro, Silvio Frascaro, Antonio Guerrieri
An amperometric biosensor based on an l-lysine-α-oxidase (LO) layer immobilized by co-crosslinking onto the surface of an overoxidized polypyrrole modified Pt electrode (Pt/oPPy) and able to analyse l-lysine (Lys) in untreated human serum is described. The sensing electrode has been characterised and a proper enzyme kinetics optimisation permits to use a low specific enzyme as LO from Trichoderma viride for the selective biorecognition of Lys in the presence of other interferent amino acids; a kinetics study of LO evidenced also the allosteric behaviour of this enzyme, a kinetic feature which was never reported before for this enzyme...
July 6, 2018: Bioelectrochemistry
Elham Salimi, Katrin Braasch, Azita Fazelkhah, Samaneh Afshar, Bahareh Saboktakin Rizi, Kaveh Mohammad, Michael Butler, Greg E Bridges, Douglas J Thomson
Nutrient depletion in fed-batch cultures and at the end of batch cultures is among the main causes of stress on cells and a trigger of apoptosis. In this study, we investigated changes in the cytoplasm conductivity of Chinese hamster ovary (CHO) cells under controlled starvation. Employing a single-cell dielectrophoresis (DEP) cytometer, we measured the DEP response of CHO cells incubated in a medium without glucose and glutamine over a 48-h period. Using the measured data in conjunction with numerical simulations, we determined the cytoplasm conductivity of viable and apoptotic cell subpopulations...
July 5, 2018: Bioelectrochemistry
O V Soldatkina, O O Soldatkin, T P Velychko, V O Prilipko, M A Kuibida, S V Dzyadevych
A new conductometric biosensor based on coimmobilized urease and arginase has been developed for arginine determination in pharmaceutics. First, the main parameters of the selected method of immobilization (concentrations of arginase, urease, and glutaraldehyde, time of incubation) were optimized. An influence of the solution parameters (buffer ionic strength, capacity, pH, Mn2+ concentration) on the biosensor operation was studied, working conditions were optimized. After biosensor optimization, the main analytical characteristics were as follows...
July 5, 2018: Bioelectrochemistry
Ezequiel Goldberg, Cecilia Suárez, Mauricio Alfonso, Juan Marchese, Alejandro Soba, Guillermo Marshall
Electroporation-based techniques, i.e. techniques based on the perturbation of the cell membrane through the application of electric pulses, are widely used at present in medicine and biotechnology. However, the electric pulse - cell membrane interaction is not yet completely understood neither explicitly formalized. Here we introduce a Multiphysics (MP) model describing electric pulse - cell membrane interaction consisting on the Poisson equation for the electric field, the Nernst-Planck equations for ion transport (protons, hydroxides, sodium or calcium, and chloride), the Maxwell tensor and mechanical equilibrium equation for membrane deformations (with an explicit discretization of the cell membrane), and the Smoluchowski equation for membrane permeabilization...
July 3, 2018: Bioelectrochemistry
Izabella Brand, Karl-Wilhelm Koch
The neuronal calcium sensor protein recoverin is expressed in retinal rod and cone cells and is involved in the calcium-dependent control of receptor (rhodopsin) phosphorylation and receptor inactivation. In its Ca2+ -saturated form recoverin is attached to membranes by an exposed myristoyl group and responds to oscillating changes of intracellular Ca2+ -concentration by performing a so-called Ca2+ -myristoyl switch. In this work we analyze changes in a liquid lipid bilayer interacting with myristoylated and non-myristoylated recoverin by employing polarization modulation infrared reflection absorption spectroscopy (PM IRRAS) with electrochemical control...
June 25, 2018: Bioelectrochemistry
Ping Zhou, Fei He, Yu Han, Bin Liu, Shicheng Wei
Nanosecond pulsed electric field (nsPEF) has the ability to induce a host of intracellular biochemical processes in living cells as a function of parameter setting. In vitro experiments proved that nsPEF stimulation could remarkably promote biomineralization processes and new bone formation. However, the impact of nsPEF parameter settings on the calcium flux of osteoblasts, as well as the intracellular mechanisms underlying those observations have yet to be elucidated. In this study, live osteoblast-like MG63 cells were loaded with fluorescence indicator dye fluo-4 AM...
June 23, 2018: Bioelectrochemistry
Yang Liu, John Daye, David Jenson, Stephen Fong
The photoelectrochemical electrode has been intensively studied in recent years as a means of generating electricity from light through the use of intact thylakoid membranes or highly purified photosystem II. PSII-enriched thylakoid membrane fragments (PSII-BBY), also have the potential to construct the photoelectrochemical anode. In this study, we examined the feasibility of utilizing PSII-BBY preparations to construct a relatively inexpensive photoelectrochemical anode with a comparable current density and a reasonable stability...
June 23, 2018: Bioelectrochemistry
N Ueoka, A Kouzuma, K Watanabe
Exoelectrogens play central roles in microbial fuel cells and other bioelectrochemical systems (BESs), yet their physiological diversity remains largely elusive due to the lack of efficient methods for the isolation from naturally occurring microbiomes. The present study developed an electrode plate-culture (EPC) method that facilitates selective colony formation by exoelectrogens and used it for isolating them from an exoelectrogenic microbiome enriched from paddy-field soil. In an EPC device, the surface of solidified agarose medium was spread with a suspension of a microbiome and covered with a transparent fluorine doped tin oxide (FTO) electrode (poised at 0 V vs...
June 22, 2018: Bioelectrochemistry
Abdelkader Zebda, Jean-Pierre Alcaraz, Pankaj Vadgama, Sergey Shleev, Shelley D Minteer, François Boucher, Philippe Cinquin, Donald K Martin
There is a growing interest in the design and engineering of operational biofuel cells that can be implanted. This review highlights the recent progress in the electrochemistry of biofuel cell technologies, but with a particular emphasis on the medical and physiological aspects that impact the biocompatibility of biofuel cells operating inside a living body. We discuss the challenge of supplying power to implantable medical devices, with regard to the limitations of lithium battery technology and why implantable biofuel cells can be a promising alternative to provide the levels of power required for medical devices...
June 18, 2018: Bioelectrochemistry
Anna Choromanska, Sandra Lubinska, Anna Szewczyk, Jolanta Saczko, Julita Kulbacka
There are still not specified mechanisms how beta-glucan molecules are transported into cells. Supposing, beta-glucan toxicity against tumor cells may be related to the overexpression of the transporter responsible for the transport of glucose molecules in the cells. In this case, glucans - polymers composed of glucose units are much more up-taken by tumor than normal cells. Increased GLUT1 (Glucose Transporter Type 1) expression has been demonstrated earlier in malignant melanomas. GLUT1 expression promotes glucose uptake and cell growth in that cells...
June 6, 2018: Bioelectrochemistry
Clément Flayac, Eric Trably, Nicolas Bernet
Microbial community structure of anodic biofilms plays a key role in bioelectrochemical systems (BESs). When ecosystems are used as inocula, many bacterial species having interconnected ecological interactions are present. The aim of the present study was to identify these interactions for the conversion of single substrates into electrical current. Dual-chamber reactors were inoculated with activated sludge and fed in batch mode with acetate, lactate, butyrate and propionate at 80 mMe- equivalents in quadruplicate...
May 28, 2018: Bioelectrochemistry
Xiang Liu, Chen Fang, Jilin Yan, Huiling Li, Yifeng Tu
The C-peptide is a co-product of pancreatic β-cells during insulin secretion; its content in body fluid is closely related to diabetes. This paper reports an immune-sensing strategy for a simple and effective assay of C-peptide based on label-free electrochemiluminescent (ECL) signaling, with high sensitivity and specificity. The basal electrode was constructed of an indium tin oxide (ITO) glass as a conductive substrate, which was decorated by Au nanoparticles (AuNPs) with hydrolysed (3-aminopropyl)trimethoxysilane as the linker...
May 23, 2018: Bioelectrochemistry
Shmuel Rozenfeld, Hanan Teller, Michal Schechter, Ravit Farber, Olga Krichevski, Alex Schechter, Rivka Cahan
The most widely reported catalyst in microbial electrochemical cells (MEC) cathodes is platinum (Pt). The disadvantages of Pt include its high cost and sensitivity to various molecules. In this research an exfoliated molybdenum di-sulfide (MoS2 -EF) catalyst was synthesized. The size of the obtained particles was 200 ± 50 nm, 50-fold smaller than the pristine MoS2 catalyst. The MoS2 -EF Raman spectrum displays the E1 2g and A1g peaks at 373 cm-1 and 399 cm-1 . Electrochemical characterization by linear sweep voltammetry (LSV) of a rotating disc electrode RDE showed that the current density of Pt in 0...
May 19, 2018: Bioelectrochemistry
Ali R Jalalvand, Sirous Ghobadi, Hector C Goicoechea, Hui-Wen Gu, Esmael Sanchooli
In this work, voltammetric data recorded at a glassy carbon electrode (GCE) were separately used to investigate the interactions of entacapone (Comtan, CAT) with human serum albumin (HSA). Then, an augmented data matrix was constructed by the combination of voltammetric and spectroscopic data and simultaneously analysed by multivariate curve resolution-alternating least squares (MCR-ALS) to obtain more information about CAT-HSA interactions. The absence of rotational ambiguities in results obtained by MCR-ALS was verified with the help of MCR-BANDS and we confirmed that the results were unambiguous and reliable...
May 16, 2018: Bioelectrochemistry
Yolina Hubenova, Rumyana Bakalska, Mario Mitov
Modification of carbonaceous materials with different conductive coatings is a successful approach to enhance their electrocatalytic activity and thus to increase the electrical outputs when used as electrodes in biofuel cells. In this study, a methodology for electrodeposition of styrylquinolinium dye on carbon felt was developed. The produced dye electrodeposits were characterized by means of AFM, ESI-MS/MS and NMR spectroscopy. The obtained data reveal that the dye forms overlaid layers consisting of monomer molecules most likely with an antiparallel orientation...
May 10, 2018: Bioelectrochemistry
Peng Zhang, Jia Liu, Youpeng Qu, Da Li, Weihua He, Yujie Feng
Nanomaterials for facilitating the microbial extracellular electron transfer (EET) process have drawn increasing attention due to their specific physical, chemical and electrical properties. This review summarizes the research advances of nanomaterials for accelerating the EET process. Nanostructured materials, including oligomer, carbon nanotube (CNT), graphene, metal, metal oxides, and polymer, exhibit numerous admirable properties such as large surface area, high electrical conductivity, and excellent catalytic activity...
May 5, 2018: Bioelectrochemistry
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