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Stefan Belicky, Hana Černocká, Tomas Bertok, Alena Holazova, Kamila Réblová, Emil Paleček, Jan Tkac, Veronika Ostatná
In recent decades, it has become clear that most of human proteins are glycosylated and that protein glycosylation plays an important role in health and diseases. At present, simple, fast and inexpensive methods are sought for clinical applications and particularly for improved diagnostics of various diseases, including cancer. We propose a label- and reagent-free electrochemical method based on chronopotentiometric stripping (CPS) analysis and a hanging mercury drop electrode for the detection of interaction of sialylated protein biomarker a prostate specific antigen (PSA) with two important lectins: Sambucus nigra agglutinin (SNA) and Maackia amurensis agglutinin (MAA)...
June 19, 2017: Bioelectrochemistry
Nancy Velasco-Alvarez, Mariano Gutiérrez-Rojas, Ignacio González
The effects of electric current on membranes associated with metabolism modifications in Aspergillus brasiliensis (niger) ATCC 9642 were studied. A 450-mL electrochemical cell with titanium ruthenium-oxide coated electrodes and packed with 15g of perlite, as inert support, was inoculated with A. brasiliensis spores and incubated in a solid inert-substrate culture (12 d; 30°C). Then, 4.5days after starting the culture, a current of 0.42mAcm(-2) was applied for 24h. The application of low-intensity electric current increased the molecular oxygen consumption rate in the mitochondrial respiratory chain, resulting in high concentrations of reactive oxygen species, promoting high lipoperoxidation levels, according to measured malondialdehyde, and consequent alterations in membrane permeability explained the high n-hexadecane (HXD) degradation rates observed here (4...
June 19, 2017: Bioelectrochemistry
Laleh Enayati Ahangar, Masoud A Mehrgardi
In the present study, a nanoporous gold platform was applied for the amplified detection of Hepatitis B virus (HBV) by an electrochemical DNA biosensor. Ferrocene as a redox reporter was covalently attached to the DNA probe and its electrochemical signal was recorded as the biosensor response. For real samples, DNA was firstly extracted from blood of patients and then amplified by polymerase chain reaction (PCR) for 5cycles. Sensitivity of this biosensor was enhanced by using nanoporous gold electrode, therefore this sensor can discriminate the genome of HBV in real sample with low PCR cycles...
June 15, 2017: Bioelectrochemistry
Shun'ichi Ishii, Shino Suzuki, Yuko Yamanaka, Angela Wu, Kenneth H Nealson, Orianna Bretschger
Microbial fuel cells (MFCs) are one of the bioelectrochemical systems that exploit microorganisms as biocatalysts to degrade organic matters and recover energy as electric power. Here, we explored how the established electrogenic microbial communities were influenced by three different inoculum sources; anaerobic sludge of the wastewater plant, rice paddy field soil, and coastal lagoon sediment. We periodically characterized both electricity generation with sucrose consumption and 16S rRNA-basis microbial community composition...
June 15, 2017: Bioelectrochemistry
Pau Batlle-Vilanova, Ramon Ganigué, Sara Ramió-Pujol, Lluís Bañeras, Gerard Jiménez, Manuela Hidalgo, M Dolors Balaguer, Jesús Colprim, Sebastià Puig
To date acetate is the main product of microbial electrosynthesis (MES) from carbon dioxide (CO2). In this work a tubular bioelectrochemical system was used to carry out MES and enhance butyrate production over the other organic products. Batch tests were performed at a fixed cathode potential of -0.8V vs SHE. The reproducibility of the results according to previous experiments was validated in a preliminary test. According to the literature butyrate production could take place by chain elongation reactions at low pH and high hydrogen partial pressure (pH2)...
June 15, 2017: Bioelectrochemistry
Mirosław Zając, Andrzej Lewenstam, Krzysztof Dolowy
Cystic Fibrosis (CF) is the most common fatal human genetic disease. It is caused by the defect in a single anion channel protein which affects ion and water transport across the epithelial tissue. A flat multi-electrode platform of diameter 12mm, allowing for measurement of four ions: sodium, potassium, hydrogen and chloride by exchangeable/replaceable ion-selective electrodes is described. The measurement is possible owing to the architecture of the platform which accommodates all the electrodes and inlets/outlets...
June 13, 2017: Bioelectrochemistry
Yunshu Zhang, Junqiu Jiang, Qingliang Zhao, YunZhi Gao, Kun Wang, Jing Ding, Hang Yu, Yue Yao
Anodic electron transfer is the predominant electricity generation process of MFCs. To accelerate anodic biofilms formation and electron transfer, 40mg/L, 80mg/L, and 120mg/L of rhamnolipid biosurfactants were added to the anolyte, resulting in an increased abiotic capacitance from 15.12F/m(2) (control) to 16.54F/m(2), 18.00F/m(2), and 19.39F/m(2), respectively. Anodic biofilm formation was facilitated after dosing 40mg/L of rhamnolipids on the 7th day after inoculation, resulting in an increased anodic biofilm coverage from 0...
June 8, 2017: Bioelectrochemistry
Nikolaos Xafenias, Cathleen Kmezik, Valeria Mapelli
It has been suggested that application of electric potential can affect lysine producing fermentations, although experimental evidence is lacking. To study this hypothesis we used the lysine producer Corynebacterium glutamicum ZW04, and we exposed it to 12 different conditions regarding anaerobic gas environment, applied electrode potential (cathodic, open circuit, anodic), redox mediator and nitrate presence. The gas environment was found to play a major role, with CO2 leading to double the lysine concentrations and yields when compared to N2...
June 6, 2017: Bioelectrochemistry
Xiaoxue Mei, Defeng Xing, Yang Yang, Qian Liu, Huihui Zhou, Changhong Guo, Nanqi Ren
Temperature as an important ecological factor affects biofilm development and microbial metabolic activity. Here, the performances and microbial communities of microbial fuel cells (MFCs) at different temperature were analyzed. As the temperature decreased, the power output of MFCs declined. A maximum power density of 894.3±48.6mW/m(2) was obtained in MFCs operating at 30°C, which was 18.5% and 64.5% higher than that in MFCs at 20°C and 10°C, respectively. Illumina sequencing of 16S rRNA gene amplicons showed that a distinct difference in microbial community structure of the anode biofilms occurred...
May 24, 2017: Bioelectrochemistry
Ya-Juan Qiao, Yan Qiao, Long Zou, Xiao-Shuai Wu, Jian-Hua Liu
Bacteria biofilm plays a key role in current generation of microbial fuel cells (MFCs), especially for the start-up stage. However, the detailed mechanism of the biofilm promoting the power generation is not very clear so far, especially for those exoelectrogens who rely on the self-excreted electron mediators for extracellular electron transfer. In this work, a biofilm formation inhibitor-sodium houttuyfonate (SH) is used to build a "non-biofilm" anode of Pseudomonas aeruginosa (P. aeruginosa) without affecting the bacteria growth during the MFC operation...
May 23, 2017: Bioelectrochemistry
Linpeng Yu, Yong Yuan, Jiahuan Tang, Shungui Zhou
Microbial electrosynthesis (MES) is a promising technique that converts electricity and CO2 to biofuels using microbes as the catalysts. However, most of previous MES are conducted at mesophilic temperatures and challenged by low performances. Here we report a significant electrosynthesis performance enhancement via immobilization of a thermophilic microbe to cathodes. A temperature-dependent electron uptake rate of Moorella thermoautotrophica was observed at a cathode potential of -0.4V (vs. SHE), with a maximum current density of 63...
May 13, 2017: Bioelectrochemistry
Jiali Wu, Wenshan Chen, Yuqing Yan, Kailin Gao, Chengmei Liao, Qiang Li, Xin Wang
Autotrophic bacteria are able to catalyze cathodic oxygen reduction as a renewable and sustainable inexpensive catalyst. However, the performance of biocathode varied over reactors, and we still not know how inoculums affect this system. Using three different inoculum of wastewater (WW), sediment extract (SE) and soil extract (SO) in parallel reactors, we found that SE achieved the shortest setup time (17-25% shorter) as well as the highest power density compared to those of SO and WW. Cyclic voltammetry (CV) further revealed that the current densities of SE biocathodes (100±1A/m(3)) was 150% and 67% higher than those of WW biocathodes (40±1A/m(3)) and SO biocathodes (65±1A/m(3))...
May 2, 2017: Bioelectrochemistry
Yaqin Zhao, Xuefeng Chu, Binsheng Yang
The electrochemical responses of human centrin 3 (HsCen3) binding with hemin were studied by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) using glassy carbon electrodes (GCEs). In CV, the formal potential (E(0')) of hemin with the addition of HsCen3 shifted from -0.51 to -0.36V (versus saturated calomel electrode, SCE), indicating that a new species of hemin-HsCen3 had formed. Upon binding with HsCen3, the redox current of hemin in CV and DPV decreased significantly. Based on their titration curves, the association constant of HsCen3 with hemin was obtained with a logK of approximately 4, which was consistent with that obtained from spectroscopy...
April 29, 2017: Bioelectrochemistry
Carola Berger, Miriam A Rosenbaum
Pseudomonas aeruginosa is able to interact with the anode of a bioelectrochemical system through redox active phenazines. Earlier studies showed that this interaction is strain and carbon source dependent. With a spontaneously formed ΔlasR mutant of P. aeruginosa PA14 and the wildtype, we investigated the connection between the complex quorum sensing network and current production. Depending on the carbon source, phenazine production and subsequently current generation are effected differently in these two populations...
April 29, 2017: Bioelectrochemistry
Laura Rago, Pierangela Cristiani, Federica Villa, Sarah Zecchin, Alessandra Colombo, Lucia Cavalca, Andrea Schievano
Dissolved oxygen (DO) at cathodic interface is a critical factor influencing microbial fuel cells (MFC) performance. In this work, three MFCs were operated with cathode under different DO conditions: i) air-breathing (A-MFC); ii) water-submerged (W-MFC) and iii) assisted by photosynthetic microorganisms (P-MFC). A plateau of maximum current was reached at 1.06±0.03mA, 1.48±0.06mA and 1.66±0.04mA, increasing respectively for W-MFC, P-MFC and A-MFC. Electrochemical and microbiological tools (Illumina sequencing, confocal microscopy and biofilm cryosectioning) were used to explore anodic and cathodic biofilm in each MFC type...
April 7, 2017: Bioelectrochemistry
Na Yang, Yueping Ren, Xiufen Li, Xinhua Wang
Anolyte acidification is a drawback restricting the electricity generation performance of the buffer-free microbial fuel cells (MFC). In this paper, a small amount of alkali-treated anion exchange resin (AER) was placed in front of the anode in the KCl mediated single-chamber MFC to slowly release hydroxyl ions (OH(-)) and neutralize the H(+) ions that are generated by the anodic reaction in two running cycles. This short-term alkaline intervention to the KCl anolyte has promoted the proliferation of electroactive Geobacter sp...
June 2017: Bioelectrochemistry
Reginald M Atkins, Timothy J Fawcett, Richard Gilbert, Andrew M Hoff, Richard Connolly, Douglas W Brown, Anthony J Llewellyn, Mark J Jaroszeski
In vivo gene electro transfer technology has been very successful both in animal models and in clinical trials over the past 20years. However, variable transfection efficiencies can produce inconsistent outcomes. This can be due to differences in tissue architecture and/or chemical composition which may effectively create unique biological environments from subject to subject that may respond differently to the identical electric pulses. This study investigates the integration of impedance spectroscopy into the gene electro transfer process to measure murine skin impedance spectra before, during (after pulse delivery), and after gene electro transfer pulse application to determine if changes in impedance correlate with reporter gene expression...
June 2017: Bioelectrochemistry
P Lamberg, J Hamit-Eminovski, M D Toscano, O Eicher-Lorka, G Niaura, T Arnebrant, S Shleev, T Ruzgas
The interface between protein and material surface is of great research interest in applications varying from implants, tissue engineering to bioelectronics. Maintaining functionality of bioelements depends greatly on the immobilization process. In the present study direct electron transfer of cellobiose dehydrogenase from Humicola insolens (HiCDH), adsorbed on four different self-assembled monolayers (SAMs) formed by 5-6 chain length carbon thiols varying in terminal group structure was investigated. By using a combination of quartz crystal microbalance with dissipation, ellipsometry and electrochemistry the formation and function of the HiCDH film was studied...
June 2017: Bioelectrochemistry
Povilas Simonis, Skirmantas Kersulis, Voitech Stankevich, Vytautas Kaseta, Egle Lastauskiene, Arunas Stirke
Saccharomyces cerevisiae yeast cells were used as a model organism to investigate the effects of various pulsed electric fields on the programed death of such cells. These were exposed to electric field pulses with field strengths (E) of up to 220kV/cm. The effects of square shaped pulses having different durations (τ=10-90ns) and different pulse numbers (pn=1-5) were then analysed. The obtained results show that nanosecond pulses can induce the death of such cells, which in turn is dependent on the electric field pulse parameters and increase with the rise in E, τ and pn...
June 2017: Bioelectrochemistry
G Mohanakrishna, Sai Kishore Butti, R Kannaiah Goud, S Venkata Mohan
A prototype bio-catalyzed electrogenic system integrated with a biological treatment process (SBR-BET) was evaluated to study specific function of anoxic condition on the electrogenic activity. A multiphasic approach was employed, where the influence of DO on bio-electrogenic activity was optimized initially, later optimal anode to cathode inter-electrode distance was enumerated. Amongst the four electrode distances evaluated, 2cm showed higher power output. Bioelectrokinetics analysis was used to validate the system performance with the experimental variation studied...
June 2017: Bioelectrochemistry
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