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Agnieszka Bala, Łukasz Górski
A novel electrochemical PNA-based biosensor for the determination of Hg(2+) is described. The receptor layer, containing single strands of polythymine PNA (peptide nucleic acid), was formed at the surface of gold electrode. Due to the presence of thymine bases and peptide bonds, an interaction between Hg(2+) ion and receptor layer occurs. The influence of chain modification - PNA vs. DNA - and type of redox marker - anionic AQMS-Na (sodium salt of anthraquinone-2-sulfonic acid) and Fe(II/III) (potassium ferri/ferrocyanide) or cationic MB (methylene blue) and RuHex (hexaammineruthenium(III) chloride) - were studied...
October 12, 2017: Bioelectrochemistry
Christy Grobbler, Bernardino Virdis, Amanda Nouwens, Falk Harnisch, Korneel Rabaey, Philip L Bond
Shewanella species respire using iron and manganese oxides as well as electrodes as solid terminal electron acceptors. Shewanella oneidenis MR-1 exploits mediated as well as direct extracellular electron transfer (EET) modes to transfer electrons at different formal potentials. These different EET modes at different potentials may utilise alternate electron transfer pathways. Therefore, we investigated how different anode potentials, providing different maximum microbial energy gains impacted S. oneidensis microbial physiology...
October 3, 2017: Bioelectrochemistry
Katja Ursic, Spela Kos, Urska Kamensek, Maja Cemazar, Janez Scancar, Simon Bucek, Simona Kranjc, Barbara Staresinic, Gregor Sersa
Interest in platinum-based chemotherapeutics such as oxaliplatin (OXA) and cisplatin (CDDP) has been reinvigorated by their newly described impacts on tumor-specific immune responses. In addition to CDDP, OXA is frequently used to treat cancers. Based on the characteristics of OXA, which are similar to those of CDDP, and the presumably more pronounced immunomodulatory effect of OXA, OXA is a candidate for electrochemotherapy (ECT). We compared the effectiveness of intratumoral ECT with OXA to that of ECT with CDDP in murine B16F10 melanoma to determine the equieffective dose...
October 3, 2017: Bioelectrochemistry
Lucie Semenec, Andrew E Laloo, Benjamin L Schulz, Ismael A Vergara, Philip L Bond, Ashley E Franks
Interspecies electron transfer (IET) occurs in many microbial communities, enabling extracellular electron exchange for syntrophic utilization of mixed resources. Various mechanisms of IET have been characterized including direct IET (DIET) and hydrogen IET (HIT) but their evolution throughout syntrophic adaptation has not been investigated through an omics approach. A syntrophic coculture of Geobacter sulfurreducens and Pseudomonas aeruginosa was established and evolved in restricted medium. The medium required cooperative metabolism due to preferential utilization of formate and fumarate by P...
October 3, 2017: Bioelectrochemistry
Hou-Yun Yang, Bai-Ling Bao, Jing Liu, Yuan Qin, Yi-Ran Wang, Kui-Zu Su, Jun-Cheng Han, Yang Mu
This study evaluated the effect of temperature on methane production by CO2 reduction during microbial electrosynthesis (MES) with a mixed-culture biocathode. Reactor performance, in terms of the amount and rate of methane production, current density, and coulombic efficiency, was compared at different temperatures. The microbial properties of the biocathode at each temperature were also analyzed by 16S rRNA gene sequencing. The results showed that the optimum temperature for methane production from CO2 reduction in MES with a mixed-culture cathode was 50°C, with the highest amount and rate of methane production of 2...
October 2, 2017: Bioelectrochemistry
Yasemin D Yilmazel, Xiuping Zhu, Kyoung-Yeol Kim, Dawn E Holmes, Bruce E Logan
Few microorganisms have been examined for current generation under thermophilic (40-65°C) or hyperthermophilic temperatures (≥80°C) in microbial electrochemical systems. Two iron-reducing archaea from the family Archaeoglobaceae, Ferroglobus placidus and Geoglobus ahangari, showed electro-active behavior leading to current generation at hyperthermophilic temperatures in single-chamber microbial electrolysis cells (MECs). A current density (j) of 0.68±0.11A/m(2) was attained in F. placidus MECs at 85°C, and 0...
October 2, 2017: Bioelectrochemistry
Niall J Finnerty, Fiachra B Bolger
Acute limb ischaemia is caused by compromised tissue perfusion and requires immediate attention to reduce the occurrence of secondary complications that could lead to amputation or death. To address this, we have developed a novel platinum (Pt)-based electrochemical oxygen (O2) device for future applications in clinical monitoring of peripheral tissue ischaemia. The effect of integrating a Pt pseudo-reference electrode into the O2 device was investigated in vitro with an optimum reduction potential of -0.80V...
September 22, 2017: Bioelectrochemistry
David Novak, Milos Mojovic, Aleksandra Pavicevic, Martina Zatloukalova, Lenka Hernychova, Martin Bartosik, Jan Vacek
Cytochrome c (cyt c) is one of the most studied conjugated proteins due to its electron-transfer properties and ability to regulate the processes involved in homeostasis or apoptosis. Here we report an electrochemical strategy for investigating the electroactivity of cyt c and its analogs with a disrupted heme moiety, i.e. apocytochrome c (acyt c) and porphyrin cytochrome c (pcyt c). The electrochemical data are supplemented with low-temperature and spin-probe electron paramagnetic resonance (EPR) spectroscopy...
September 21, 2017: Bioelectrochemistry
Dominique Darimont, Martin J Weissenborn, Bernd A Nebel, Bernhard Hauer
Electrochemical in vitro reduction of P450 enzymes is a promising alternative to in vivo applications. Previously we presented three engineered P450BM3 variants for aniline hydroxylation, equipped with a carbon nanotube binding-peptide (CNT-tag) for self-assembly on CNT electrodes. Compared to wildtype P450BM3 the NADPH-dependent activity was enhanced, but the coupling efficiency remained low. For P450BM3 Verma, Schwaneberg and Roccatano (2014, Biopolymers 101, 197-209) calculated putative electron transfer pathways (eTPs) by MD simulations...
September 18, 2017: Bioelectrochemistry
Matthew D Yates, Brian J Eddie, Nikolai Lebedev, Nicholas J Kotloski, Sarah M Strycharz-Glaven, Leonard M Tender
The ability of certain microorganisms to live in a multi-cell thick, electrode-grown biofilm by utilizing the electrode as a metabolic electron acceptor or donor requires electron transfer across cell membranes, through the biofilm, and across the biofilm/electrode interface. Even for the most studied system, anode-grown Geobacter sulfurreducens, the mechanisms underpinning each process and how they connect is largely unresolved. Here we report on G. sulfurreducens biofilms grown across the gap separating two electrodes by maintaining one electrode at 0...
September 18, 2017: Bioelectrochemistry
Vitalij Novickij, Paulius Ruzgys, Audrius Grainys, Saulius Šatkauskas
The study presents the proof of concept for a possibility to achieve a better electroporation in the MHz pulse repetition frequency (PRF) region compared to the conventional low frequency protocols. The 200ns×10 pulses bursts of 10-14kV/cm have been used to permeabilize Chinese hamster ovary (CHO) cells in a wide range (1Hz-1MHz) of PRF. The permeabilization efficiency was evaluated using fluorescent dye assay (propidium iodide) and flow cytometry. It was determined that a threshold PRF exists when the relaxation of the cell transmembrane potential is longer than the delay between the consequent pulses, which results in accumulation of the charge on the membrane...
September 14, 2017: Bioelectrochemistry
Marie Breton, Lluis M Mir
The chemical consequences of electropulsation on giant unilamellar vesicles (GUVs), in particular the possible oxidation of unsaturated phospholipids, have been investigated by mass spectrometry, flow cytometry and absorbance methods. Pulse application induced oxidation of the GUV phospholipids and the oxidation level depended on the duration of the pulse. Light and O2 increased the level of pulse-induced lipid peroxidation whereas the presence of antioxidants either in the membrane or in the solution completely suppressed peroxidation...
September 11, 2017: Bioelectrochemistry
Yunshu Zhang, Junqiu Jiang, Qingliang Zhao, Kun Wang, Hang Yu
Extracellular electron transfer is the predominant electricity generation process in microbial fuel cells (MFCs). Our pervious study have proved that the anodic adsorption of rhamnolipid resulted in the Frumkin effect, which enhanced anodic microorganism attachment and accelerated anodic electron transfer. In this study, an in-depth research on the influence of rhamnolipid on functional genes of anodic biofilms metagenomes was carried out to explain its mechanism at the gene level. The result showed that the composition and distribution of functional genes in each dominant genus were different...
September 11, 2017: Bioelectrochemistry
Lynn Carr, Sylvia M Bardet, Delia Arnaud-Cormos, Philippe Leveque, Rodney P O'Connor
Cytosolic, synthetic chemical calcium indicators are typically used to visualise the rapid increase in intracellular calcium ion concentration that follows nanosecond pulsed electric field (nsPEF) application. This study looks at the application of genetically encoded calcium indicators (GECIs) to investigate the spatiotemporal nature of nsPEF-induced calcium signals using fluorescent live cell imaging. Calcium responses to 44kV/cm, 10ns pulses were observed in U87-MG cells expressing either a plasma membrane targeted GECI (GCaMP5-G), or one cytosolically expressed (GCaMP6-S), and compared to the response of cells loaded with cytosolic or plasma membrane targeted chemical calcium indicators...
September 8, 2017: Bioelectrochemistry
Lianqiang Li, Fei Ding, Lin Sang, Jiaquan Liu, Duolu Mao, Xingjiang Liu, Qiang Xu
As the development of marine economy, the submarine battery with the seawater electrolyte has obtained more and more attentions. Owing to the conventional electrochemical catalysts of the cathodes in seawater battery are expensive, it is to seek the new biological catalysts to improve the electrochemical performance of the cathode and reduce the cost of seawater battery. A novel marine bacterial strain (Strain SQ-32) phylogenetically related to the Erythrobactercitreus strain has been isolated from the sea-bed sludge in the Yellow Sea of China successfully...
September 8, 2017: Bioelectrochemistry
Maria Gamella, Ashkan Koushanpour, Evgeny Katz
The article represents a short conceptual overview of biofuel cell applications, rather than their design and operation. Special attention is given to interfacing enzyme-based biofuel cells with power consuming microelectronic devices. Importance of electronic management of the power extracted from biological sources is emphasized. In addition to several briefly explained examples collected from recent publications, one system demonstrating powering of a standard glucometer with an implantable or wearable biofuel cell is described in details...
September 7, 2017: Bioelectrochemistry
Jules-Blaise Mabou Leuna, Sergeot Kungo Sop, Suzanne Makota, Evangeline Njanja, Thiery Christophe Ebelle, Anatole Guy Azebaze, Emmanuel Ngameni, Achille Nassi
The electrochemical oxidation of Mammeisin (MA) was studied in a solution containing acetone and 0.1M phosphate buffer +0.1M KCl (pH=5.3) at a glassy carbon electrode (GCE), using cyclic (CV) and square wave voltammetry (SWV). MA showed a quasi-reversible process, which is pH dependent and that involves the exchange of two electrons and two protons. The oxidation product was adsorbed by the electrode surface to form a film that blocks active sites over repetitive cyclic. Moreover, the interaction of MA and bovine serum albumin (BSA) was studied by CV and SWV at different pHs (5...
September 7, 2017: Bioelectrochemistry
Damien Voyer, Aude Silve, Lluis M Mir, Riccardo Scorretti, Clair Poignard
In this paper, we propose a new dynamical model of tissue electroporation. The model is based on equivalent circuit approach at the tissue. Considering two current densities from cells and extracellular matrix, we identify the macroscopic homogenised contribution of the cell membranes. Our approach makes it possible to define a macroscopic homogenised electric field and a macroscopic homogenised transmembrane potential. This provides a direct link between the cell scale electroporation models and the tissue models...
September 5, 2017: Bioelectrochemistry
A C Sabuncu, M Stacey, G L Craviso, N Semenova, P T Vernier, N Leblanc, I Chatterjee, J Zaklit
Knowledge of the dielectric properties of biological cells plays an important role in numerical models aimed at understanding how high intensity ultrashort nanosecond electric pulses affect the plasma membrane and the membranes of intracellular organelles. To this end, using electrical impedance spectroscopy, the dielectric properties of isolated, neuroendocrine adrenal chromaffin cells were obtained. Measured impedance data of the cell suspension, acquired between 1kHz and 20MHz, were fit into a combination of constant phase element and Cole-Cole models from which the effect of electrode polarization was extracted...
September 5, 2017: Bioelectrochemistry
Manuel A Gacitúa, Enyelbert Muñoz, Bernardo González
Microbial electrolysis batch reactor systems were studied employing different conditions, paying attention on the effect that biocathode potential has on pH and system performance, with the overall aim to distinguish sulphate reduction from H2 evolution. Inocula from pure strains (Desulfovibrio paquesii and Desulfobacter halotolerans) were compared to a natural source conditioned inoculum. The natural inoculum possess the potential for sulphate reduction on serum bottles experiments due to the activity of mutualistic bacteria (Sedimentibacter sp...
August 25, 2017: Bioelectrochemistry
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