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Alexander G Volkov
The Venus flytrap captures insects with one of the most rapid movements in the plant kingdom. There is a significant difference between properties of electrical signals generated in the Venus flytrap described in literature. Amplitudes of action potentials vary from 14 mV to 200 mV with duration of signals from 2 ms to 10 s. Here we present experimental study of potential differences between Ag/AgCl electrodes inserted to the trap, petiole, and into soil or external ECG electrodes attached to surfaces of the Venus flytrap...
September 5, 2018: Bioelectrochemistry
Paola Brun, Monica Dettin, Luca Giovanni Campana, Fabrizio Dughiero, Paolo Sgarbossa, Clara Bernardello, Anna Lisa Tosi, Annj Zamuner, Elisabetta Sieni
Electroporation of cells is usually studied using cell suspensions or monolayer cultures. 3D scaffolds for cell culture have been recently designed in order to reproduce in vitro the complex and multifactorial environment experimented in vivo by cells. In fact, it is well known that 2D cell cultures are not able to simulate the complex interactions between the cells and their extracellular matrix (ECM). Recently, some examples of 3D models, like spheroids, have been investigated also in the electroporation field...
August 30, 2018: Bioelectrochemistry
Filipa O Gomes, Luísa B Maia, Cristina Cordas, Isabel Moura, Cristina Delerue-Matos, José J G Moura, Simone Morais
Understanding the direct electron transfer processes between redox proteins and electrode surface is fundamental to understand the proteins mechanistic properties and for development of novel biosensors. In this study, nitric oxide reductase (NOR) extracted from Marinobacter hydrocarbonoclasticus bacteria was adsorbed onto a pyrolytic graphite electrode (PGE) to develop an unmediated enzymatic biosensor (PGE/NOR)) for characterization of NOR direct electrochemical behaviour and NOR electroanalytical features towards NO and O2 ...
August 21, 2018: Bioelectrochemistry
Mi Yeon Kim, Changman Kim, Satish Kumar Ainala, Hyokwan Bae, Byong-Hun Jeon, Sunghoon Park, Jung Rae Kim
Klebsiella pneumoniae is used widely for the production of value-added chemicals from glycerol, and is known as an exoelectrogen with an externally provided electron shuttle. In this study, the metabolic shift in K. pneumoniae L17 by the activation of electrode-based respiration was examined using microbial fuel cells (MFCs). The mRNA expression levels of the related enzymes for glycerol conversion were compared under electrode-driven anaerobic respirational conditions (i.e., MFC) and fermentative conditions (i...
August 13, 2018: Bioelectrochemistry
D Zouraris, M Dimarogona, A Karnaouri, E Topakas, A Karantonis
MtLPMO9 and FoLPMO9 are two lytic polysaccharide monooxygenases (LPMOs), from the filamentous fungi Thermothelomyces thermophila and Fusarium oxysporum, respectively. In the present study an attempt has been made to achieve direct electron transfer between these enzymes and a glassy carbon electrode by immobilization in Nafion polyelectrolyte. The method used to ascertain the feasibility of direct electron transfer was large amplitude Fourier transform alternating current voltammetry (FTacV) and the formal potentials of these enzymes were determined at different temperatures...
July 19, 2018: Bioelectrochemistry
Marcin Kruszewski, Magdalena Kusaczuk, Joanna Kotyńska, Miroslav Gál, Rafał Krętowski, Marzanna Cechowska-Pasko, Monika Naumowicz
Quercetin is a naturally-occurring flavonoid claimed to exert many beneficial health effects. In this report, the influence of quercetin on the surface charge of phosphatidylcholine liposomes and human glioblastoma LN-229 and LN-18 cells was studied using microelectrophoretic mobility measurements. The effect of quercetin on the electrical resistance and capacitance of bilayer lipid membranes was analyzed via electrochemical impedance spectroscopy. The results showed that after flavonoid treatment, the cell lines demonstrated changes in surface charge only in alkaline pH solutions, whereas there were no significant alterations in quercetin-treated vs...
July 17, 2018: Bioelectrochemistry
José Luis Olloqui-Sariego, Antonio Díaz-Quintana, Miguel Ángel De la Rosa, Juan José Calvente, Inmaculada Márquez, Irene Díaz-Moreno, Rafael Andreu
Increasing the thermal stability of immobilized proteins is a motivating goal for improving the performance of electrochemical biodevices. In this work, we propose the immobilization of crosslinked plastocyanin from the thermophilic cyanobacterium Phormidium laminosum by simultaneous incubation of a mixture of plastocyanin and the coupling reagents. The thermal stability of the so built covalently immobilized protein films has been assessed by cyclic voltammetry in the 0-90 °C temperature range and has been compared to that of physisorbed films...
July 17, 2018: Bioelectrochemistry
Shuai Liang, Baogang Zhang, Jiaxin Shi, Ting Wang, Lifeng Zhang, Zhijun Wang, Cuibai Chen
Electrochemical decolorization is of particular importance for the efficient treatment of dye wastewater. A promising electrochemical system powered by microbial fuel cells (MFCs) and intensified by Fe-C micro-electrolysis is proposed and enhanced decolorization of methyl orange (MO) is realized in this study. The decolorization efficiency reached as high as 97.1 ± 1.8% after 180 min of operation with initial MO concentration of 50 mg/L and applied voltage of 700 mV. Decolorization efficiency initially increased and then decreased with rising Fe-to-C ratio...
July 10, 2018: Bioelectrochemistry
Anna A Bulysheva, Nina Burcus, Cathryn G Lundberg, Michael P Francis, Richard Heller
Atherosclerosis induced myocardial infarction (MI) continues to be a major public health concern. Regenerative therapies that restore cardiac muscle cells are largely absent. The rate of cardiomyogenesis in adults is insufficient to compensate for MI damage. In this study, we explored the capacity of a gene therapy approach to promote cardiomyogenesis. We hypothesized that VEGF-B, critical during fetal heart development, could promote cardiomyogenesis in adult ischemic hearts. Gene electrotransfer (GET), a physical method of in vivo gene delivery, was adapted to the rat model of MI...
July 10, 2018: Bioelectrochemistry
Qiang Yuan, Y Huang, Debao Liu, Minfang Chen
Biodegradable beta-tricalcium phosphate (β-TCP) particle reinforced magnesium metal matrix composites (Mg-MMC) have attracted increasing interest for application as implant materials. This investigation was conducted to study the effect of cooling rate on the microstructure and corrosion behavior of a biodegradable β-TCP/Mg-Zn-Ca composite. The composite was fabricated under a series of cooling rates using a wedge-shaped casting mold. The microstructure of the composite was examined by optical and scanning electron microscopy, and the corrosion behavior was investigated using an electrochemical workstation and immersion tests in a simulated body fluid (SBF)...
July 7, 2018: Bioelectrochemistry
Shiue-Lin Li, Jui-Hung Yen, Kenji Kano, Shiu-Mei Liu, Chien-Liang Liu, Sheng-Shung Cheng, Hsun-Yi Chen
Using an electrochemical cell equipped with carbon felt electrodes (poised at +0.63 V vs. SHE), the current production capabilities of two Shewanella strains-NTOU1 and KR-12-were examined under various conditions with lactate as an electron donor. The metabolic charge produced in the tricarboxylic acid cycle (QTCA ) was calculated by mass-balance. The data showed a linear relation between the electric coulomb production (QEL ) and QTCA with an R2 of 0.65. In addition, a large amount of pyruvate accumulation was observed at pH = 6, rendering QTCA negative...
July 6, 2018: Bioelectrochemistry
Rosanna Ciriello, Francesca De Gennaro, Silvio Frascaro, Antonio Guerrieri
An amperometric biosensor based on an l-lysine-α-oxidase (LO) layer immobilized by co-crosslinking onto the surface of an overoxidized polypyrrole modified Pt electrode (Pt/oPPy) and able to analyse l-lysine (Lys) in untreated human serum is described. The sensing electrode has been characterised and a proper enzyme kinetics optimisation permits to use a low specific enzyme as LO from Trichoderma viride for the selective biorecognition of Lys in the presence of other interferent amino acids; a kinetics study of LO evidenced also the allosteric behaviour of this enzyme, a kinetic feature which was never reported before for this enzyme...
July 6, 2018: Bioelectrochemistry
Elham Salimi, Katrin Braasch, Azita Fazelkhah, Samaneh Afshar, Bahareh Saboktakin Rizi, Kaveh Mohammad, Michael Butler, Greg E Bridges, Douglas J Thomson
Nutrient depletion in fed-batch cultures and at the end of batch cultures is among the main causes of stress on cells and a trigger of apoptosis. In this study, we investigated changes in the cytoplasm conductivity of Chinese hamster ovary (CHO) cells under controlled starvation. Employing a single-cell dielectrophoresis (DEP) cytometer, we measured the DEP response of CHO cells incubated in a medium without glucose and glutamine over a 48-h period. Using the measured data in conjunction with numerical simulations, we determined the cytoplasm conductivity of viable and apoptotic cell subpopulations...
July 5, 2018: Bioelectrochemistry
O V Soldatkina, O O Soldatkin, T P Velychko, V O Prilipko, M A Kuibida, S V Dzyadevych
A new conductometric biosensor based on coimmobilized urease and arginase has been developed for arginine determination in pharmaceutics. First, the main parameters of the selected method of immobilization (concentrations of arginase, urease, and glutaraldehyde, time of incubation) were optimized. An influence of the solution parameters (buffer ionic strength, capacity, pH, Mn2+ concentration) on the biosensor operation was studied, working conditions were optimized. After biosensor optimization, the main analytical characteristics were as follows...
July 5, 2018: Bioelectrochemistry
Ezequiel Goldberg, Cecilia Suárez, Mauricio Alfonso, Juan Marchese, Alejandro Soba, Guillermo Marshall
Electroporation-based techniques, i.e. techniques based on the perturbation of the cell membrane through the application of electric pulses, are widely used at present in medicine and biotechnology. However, the electric pulse - cell membrane interaction is not yet completely understood neither explicitly formalized. Here we introduce a Multiphysics (MP) model describing electric pulse - cell membrane interaction consisting on the Poisson equation for the electric field, the Nernst-Planck equations for ion transport (protons, hydroxides, sodium or calcium, and chloride), the Maxwell tensor and mechanical equilibrium equation for membrane deformations (with an explicit discretization of the cell membrane), and the Smoluchowski equation for membrane permeabilization...
July 3, 2018: Bioelectrochemistry
Izabella Brand, Karl-Wilhelm Koch
The neuronal calcium sensor protein recoverin is expressed in retinal rod and cone cells and is involved in the calcium-dependent control of receptor (rhodopsin) phosphorylation and receptor inactivation. In its Ca2+ -saturated form recoverin is attached to membranes by an exposed myristoyl group and responds to oscillating changes of intracellular Ca2+ -concentration by performing a so-called Ca2+ -myristoyl switch. In this work we analyze changes in a liquid lipid bilayer interacting with myristoylated and non-myristoylated recoverin by employing polarization modulation infrared reflection absorption spectroscopy (PM IRRAS) with electrochemical control...
June 25, 2018: Bioelectrochemistry
Alexander G Volkov, Yuri B Shtessel
According to literature, electrostimulation of plants can induce plant movement, activation of ion channels, ion transport, gene expression, enzymatic system activation, electrical signaling, plant-cell damage, enhanced wound healing, and can also influence plant growth. Many plants can communicate above ground and underground between adjacent plants. Electrostimulation by square pulses induces passive electrotonic potentials propagating within and between tomato plants. The amplitude and sign of electrotonic potentials, in both the electrostimulated and neighboring tomato plants depends on the amplitude, rise and fall of the applied voltage...
December 2018: Bioelectrochemistry
Stéphane Pinck, Mengjie Xu, Romain Clement, Elisabeth Lojou, Frédéric P A Jorand, Mathieu Etienne
An electroactive artificial biofilm has been optimized for the cathodic reduction of fumarate by Shewanella oneidensis. The system is based on the self-assembly of multi-walled carbon nanotubes with bacterial cells in the presence of a c-type cytochrome. The aggregates are then deposited on an electrode to form the electroactive artificial biofilm. Six c-type cytochromes have been studied, from bovine heart or Desulfuromonas and Desulfuvibrio strains. The isoelectric point of the cytochrome controls the self-assembly process that occurs only with positively-charged cytochromes...
December 2018: Bioelectrochemistry
Saleheh Abbasi, Ali Benvidi, Sajjad Gharaghani, Masoud Rezaeinasab
Thymol (Thy) is a hydrophobic active ingredient present in thyme essential oils. It is marginally soluble in water which is one of the challenging limitations for its application in foods or as drugs. An important aspect of our understanding from this system is to quantitatively comprehend how albumin interacts with Thy. Herein, this study has been focused on the interactions between thymol and bovine serum albumin (BSA) using electrochemical methods and ultraviolet and visible (UV-Vis) spectroscopy. Due to the overlap between obtained signals of existing species, it is apparent that multivariate methods can resolve overlapping signal trough unique decomposition...
December 2018: Bioelectrochemistry
Maryam Hosseini Ghalehno, Mohammad Mirzaei, Masoud Torkzadeh-Mahani
In this work an electrochemical label free DNA biosensor (ds-DNA) for the determination of menadione (MD) was developed. The biosensor was constructed using a modified nanocomposite consisting of Fe3 O4 nanoparticles decorated reduced graphene oxide (Gr) on a carbon paste electrode (CPE). Scanning electron microscope (SEM), energy dispersive X-ray (EDAX) and Fourier transform infrared (FT-IR) spectroscopy confirmed the structure of the synthesized nanocomposites (electrode composition). The Gr-Fe3 O4 nanocomposites formed a sensitive layer with large surface area...
December 2018: Bioelectrochemistry
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