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Francisco Lopez Moruno, Juan E Rubio, Plamen Atanassov, José M Cerrato, Christopher G Arges, Carlo Santoro
Microbial desalination cell (MDC) is a bioelectrochemical system capable of oxidizing organics, generating electricity, while reducing the salinity content of brine streams. As it is designed, anion and cation exchange membranes play an important role on the selective removal of ions from the desalination chamber. In this work, sulfonated sodium (Na+ ) poly(ether ether ketone) (SPEEK) cation exchange membranes (CEM) were tested in combination with quaternary ammonium chloride poly(2,6-dimethyl 1,4-phenylene oxide) (QAPPO) anion exchange membrane (AEM)...
February 9, 2018: Bioelectrochemistry
Ana S Peinetti, Helena Ceretti, Martín Mizrahi, Graciela A González, Silvana A Ramírez, Félix G Requejo, Javier M Montserrat, Fernando Battaglini
Polyvalent gold nanoparticle oligonucleotide conjugates are subject of intense research. Even though 2nm diameter AuNPs have been previously modified with DNA, little is known about their structure and electrochemical behavior. In this work, we examine the influence of different surface modification strategies on the interplay between the meso-organization and the molecular recognition properties of a 27-mer DNA strand. This DNA strand is functionalized with different sulfur-containing moieties and immobilized on 2nm gold nanoparticles confined on a nanoporous alumina, working the whole system as an electrode array...
February 9, 2018: Bioelectrochemistry
F Kubannek, U Schröder, U Krewer
In this work we employ differential electrochemical mass spectrometry (DEMS) in combination with static and dynamic electrochemical techniques for the study of metabolic processes of electrochemically active bacteria. CO 2 production during acetate oxidation by electrode respiring bacteria was measured, in-vivo and online with a sensitivity of 6.5 ⋅ 10 -13 mol/s. The correlation of ion current and electrical current provides insight into the interaction of metabolic processes and extra-cellular electron transfer...
February 8, 2018: Bioelectrochemistry
Shanshan Chen, Yanlun Fang, Xianyue Jing, Hailin Luo, Jing Chen, Shungui Zhou
Microbial electrosynthesis systems (MES) are promising devices in which microbes obtain electrons from electrodes to produce extracellular multicarbon compounds. This study investigates whether improvement in cell permeability can enhance electrosynthesis performance of Gram-positive Moorella thermoautotrophica in MES. Results showed that when ≤30mg/L penicillin was added, the cell permeability was doubled, and the electron uptake per biomass (including both cathode-associated biomass and suspended biomass) was 1...
February 8, 2018: Bioelectrochemistry
Ariadna Vilar-Sanz, Narcís Pous, Sebastià Puig, M Dolors Balaguer, Jesús Colprim, Lluís Bañeras
No abstract text is available yet for this article.
February 2, 2018: Bioelectrochemistry
Nuno R Ferreira, Ana Ledo, João Laranjinha, Greg A Gerhardt, Rui M Barbosa
Nanocomposite sensors consisting of carbon fiber microelectrodes modified with Nafion® and carbon nanotubes, and ceramic-based microelectrode biosensor arrays were used to measure ascorbate and glutamate in the brain with high spatial, temporal and chemical resolution. Nanocomposite sensors displayed electrocatalytic properties towards ascorbate oxidation, translated into a negative shift from +0.20V to -0.05V vs. Ag/AgCl, as well as a significant increase (10-fold) of electroactive surface area. The estimated average basal concentration of ascorbate in vivo in the CA1, CA3 and dentate gyrus (DG) sub regions of the hippocampus were 276±60μM (n=10), 183±30μM (n=10) and 133±42μM (n=10), respectively...
January 31, 2018: Bioelectrochemistry
Iurii Semenov, Maura Casciola, Bennet L Ibey, Shu Xiao, Andrei G Pakhomov
Decreasing the time gap between two identical electric pulses is expected to render bioeffects similar to those of a single pulse of equivalent total duration. In this study, we show that it is not necessarily true, and that the effects vary for different permeabilization markers. We exposed individual CHO or NG108 cells to one 300-ns pulse (3.7-11.6 kV/cm), or a pair of such pulses (0.4-1000 μs interval), or to a single 600-ns pulse of the same amplitude. Electropermeabilization was evaluated (a) by the uptake of YO-PRO-1 (YP) dye; (b) by the amplitude of elicited Ca2+ transients, and (c) by the entry of Tl+ ions...
January 31, 2018: Bioelectrochemistry
Hongjuan Wang, Duo Qian, Xilin Xiao, Chunyan Deng, Lifu Liao, Jian Deng, Ying-Wu Lin
An innovative electrochemical sensor was fabricated for the sensitive and selective determination of tinidazole (TNZ), based on a carbon paste electrode (CPE) modified with multi-walled carbon nanotubes (MWCNTs) and boron-embedded molecularly imprinted composite membranes (B-MICMs). Density functional theory (DFT) calculations were carried out to investigate the utility of template-monomer interactions to screen appropriate monomers for the rational design of B-MICMs. The distinct synergic effect of MWCNTs and B-MICMs was evidenced by the positive shift of the reduction peak potential of TNZ at B-MICMs/MWCNTs modified CPE (B-MICMs/MWCNTs/CPE) by about 200 mV, and the 12-fold amplification of the peak current, compared with a bare carbon paste electrode (CPE)...
January 31, 2018: Bioelectrochemistry
Zimu Xu, Cheng Cheng, Jie Shen, Yan Lan, Shuheng Hu, Wei Han, Paul K Chu
The direct inactivation effects of an atmospheric pressure direct current (DC) air plasma against planktonic Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) in aqueous solution are investigated in vitro. Upon plasma treatment, extensively analyses on cell culturability, metabolic capacity, membrane integrity, surface morphology, cellular proteins, nucleic acids and intracellular reactive oxygen species (ROS) for both bacterial species were carried out and significant antimicrobial effects observed...
January 30, 2018: Bioelectrochemistry
B Jachimska, S Świątek, J I Loch, K Lewiński, T Luxbacher
Bovine β-lactoglobulin (LGB) is a transport protein that can bind to its structure hydrophobic bioactive molecules. Due to the lack of toxicity, high stability and pH-dependent molecular binding mechanism, lactoglobulin can be used as a carrier of sparingly soluble drugs. Dynamic light scattering has confirmed LGB's tendency to create oligomeric forms. The hydrodynamic diameter of LGB molecules varies from 4 nm to 6 nm in the pH range of 2-10 and ionic strength I = 0.001-0.15 M, which corresponds to the presence of mono or dimeric LGB forms...
January 29, 2018: Bioelectrochemistry
Sebastian Schneider, Michael Rudolph, Vanessa Bause, Andreas Terfort
The infection of dental implants may cause severe inflammation of tissue and even bone degradation if not treated. For titanium implants, a new, minimally invasive approach is the electrochemical removal of the biofilms including the disinfection of the metal surface. In this project, several parameters, such as electrode potentials and electrolyte compositions, were varied to understand the underlying mechanisms. Optimal electrolytes contained iodide as well as lactic acid. Electrochemical experiments, such as cyclic voltammetry or measurements of open circuit potentials, were performed in different cell set-ups to distinguish between different possible reactions...
January 29, 2018: Bioelectrochemistry
Jianfang Li, Xiaojing He, Guannan Zhang, Ruiqiang Hang, Xiaobo Huang, Bin Tang, Xiangyu Zhang
Ti-6Al-4V (TC4) has received increasing attention as biomaterial but also raised concerns about the long-term safety of releasing of metal ions and poor wear resistance. In this work, an ZrO2/TiO2 alloyed layer was prepared on TC4 by plasma surface alloying with Zr and subsequently annealed in the air for improved corrosion and wear resistant. To assess the corrosion performance of the alloyed layer, the specimens were measured by open circuit potential, electrochemical impedance spectroscopy and potentiodynamic polarization in simulated body fluid solution...
January 29, 2018: Bioelectrochemistry
Mohammad Mazloum-Ardakani, Behnaz Barazesh, Alireza Khoshroo, Mohammad Moshtaghiun, Mohammad Hasan Sheikhha
In this work we report the synthesis of a stable composite with excellent electrical properties, on the surface of a biosensor. Conductive polymers offer both high electrical conductivity and mechanical strength. Many reports have focused on synthesizing conductive polymers with the aid of high-cost enzymes. In the current work we introduce a novel electrochemical, one-step, facile and cost effective procedure for synthesizing poly (catechol), without using expensive enzymes. The poly (catechol) conductivity was enhanced by modification with graphene sheets and biosynthesized gold nanoparticles...
January 16, 2018: Bioelectrochemistry
Namita Shrestha, Govinda Chilkoor, Joseph Wilder, Zhiyong Jason Ren, Venkataramana Gadhamshetty
This study evaluates and compares the performance of microbial fuel cells (MFCs) and microbial capacitive deionization cells (MCDCs) fed with wastewater produced from the Bakken shale. The produced water was characterized by high levels of dissolved solids and chemical oxygen demand (COD). Two-compartment MFCs and three-compartment MCDCs were evaluated under batch-fed mode using mixed microbial consortia in the anode, ferricyanide in the cathode, and produced water as the electrolyte in the anode and capacitive deionization units...
January 11, 2018: Bioelectrochemistry
Lei Chen, Peng Zhang, Weitao Shang, Hongxia Zhang, Yuntao Li, Weiguo Zhang, Zhaojie Zhang, Fanghua Liu
Many electroactive microorganisms (EAMs) are known as dissimilatory iron-reducing bacteria (DIRB) which can access iron as an electron acceptor to transfer electrons during the process of respiration. Previous research has shown that magnetic susceptibility (χlf) could be used as an indicator of DIRB activities. However, the relationship between χlf and electroactivity of EAMs is still unclear. Here, we report that after sediments of the Sha River enriched with amorphous iron, the χlf of enrichment cultures increased dramatically...
January 10, 2018: Bioelectrochemistry
Raúl Mateos, Ana Sotres, Raúl M Alonso, Adrián Escapa, Antonio Morán
This study seeks to understand how the bacterial communities that develop on biocathodes are influenced by inocula diversity and electrode potential during start-up. Two different inocula are used: one from a highly diverse environment (river mud) and the other from a low diverse milieu (anaerobic digestion). In addition, both inocula were subjected to two different polarising voltages: oxidative (+0.2 V vs. Ag/AgCl) and reductive (-0.8 V vs. Ag/AgCl). Bacterial communities were analysed by means of high throughput sequencing...
January 7, 2018: Bioelectrochemistry
Roxana-Mihaela Apetrei, Geta Carac, Gabriela Bahrim, Almira Ramanaviciene, Arunas Ramanavicius
The enhancement of bioelectrochemical properties of microorganism by in situ formation of conducting polymer within the cell structures (e.g. cell wall) was performed. The synthesis of polypyrrole (Ppy) within fungi (Aspergillus niger) cells was achieved. Two different Aspergillus niger strains were selected due to their ability to produce glucose oxidase, which initiated the Ppy formation through products of enzymatic reaction. The evolution of Ppy structural features was investigated by absorption spectroscopy, cyclic voltammetry and Fourier transform infrared spectroscopy...
January 6, 2018: Bioelectrochemistry
Alexey Tyulenev, Galina Smirnova, Nadezda Muzyka, Vadim Ushakov, Oleg Oktyabrsky
Real-time monitoring of the state of bacterial cultures is important in both experiment and biotechnology. Using Eh and sulfide sensors, we demonstrated that the abrupt reversible reduction in Eh (Eh jump), occurring during transition of E. coli from exponential growth to starvation and antibiotic-induced stresses, is the result of sulfide excretion from the cells. Changes in the potential of sensors had a two-phase mode. The potential reduced within 10-15min and returned within 10-30min. In the parental strain, maximum amplitudes of Eh jumps (ΔEh) were 25±2mV, 57±6mV and 36±7mV under isoleucine starvation, glucose depletion and ciprofloxacin exposure that corresponded to 43±3nM, 96±5nM and 140±1nM of sulfide, respectively...
January 3, 2018: Bioelectrochemistry
Li Fu, Aiwu Wang, Fucong Lyv, Guosong Lai, Huaiwei Zhang, Jinhong Yu, Cheng-Te Lin, Aimin Yu, Weitao Su
A chitosan based hydrogel has been fabricated using silver ions as crosslinking agent. Silver redox behavior in the hydrogel is suppressed due to complexation. However, hydrogen peroxide induced hydroxyl radicals could attract the glucoside bonds and consequently restore silver redox behavior. Therefore, we used this hydroxyl radical induced chitos and epolymerization mechanism as an indicator for antioxidant capacity evaluation. Therefore, we used this hydroxyl radical induced chitos and epolymerization as an indicator for antioxidant capacity evaluation...
December 27, 2017: Bioelectrochemistry
Hongxia Wan, Dongdong Song, Dawei Zhang, Cuiwei Du, Dake Xu, Zhiyong Liu, De Ding, Xiaogang Li
The corrosion of X80 pipeline steel in the presence of Bacillus cereus (B. cereus) was studied through electrochemical and surface analyses and live/dead staining. Scanning electron microscopy and live/dead straining results showed that a number of B. cereus adhered to the X80 steel. Electrochemical impedance spectroscopy showed that B. cereus could accelerate the corrosion of X80 steel. In addition, surface morphology observations indicated that B. cereus could accelerate pitting corrosion in X80 steel. The depth of the largest pits due to B...
December 24, 2017: Bioelectrochemistry
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