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Bioelectrochemistry

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https://www.readbyqxmd.com/read/29778043/investigation-of-interactions-of-comtan-with-human-serum-albumin-by-mathematically-modeled-voltammetric-data-a-study-from-bio-interaction-to-biosensing
#1
Ali R Jalalvand, Sirous Ghobadi, Hector C Goicoechea, Hui-Wen Gu, Esmael Sanchooli
In this work, voltammetric data recorded at a glassy carbon electrode (GCE) were separately used to investigate the interactions of entacapone (Comtan, CAT) with human serum albumin (HSA). Then, an augmented data matrix was constructed by the combination of voltammetric and spectroscopic data and simultaneously analysed by multivariate curve resolution-alternating least squares (MCR-ALS) to obtain more information about CAT-HSA interactions. The absence of rotational ambiguities in results obtained by MCR-ALS was verified with the help of MCR-BANDS and we confirmed that the results were unambiguous and reliable...
May 16, 2018: Bioelectrochemistry
https://www.readbyqxmd.com/read/29778044/electrodeposited-styrylquinolinium-dye-as-molecular-electrocatalyst-for-coupled-redox-reactions
#2
Yolina Hubenova, Rumyana Bakalska, Mario Mitov
Modification of carbonaceous materials with different conductive coatings is a successful approach to enhance their electrocatalytic activity and thus to increase the electrical outputs when used as electrodes in biofuel cells. In this study, a methodology for electrodeposition of styrylquinolinium dye on carbon felt was developed. The produced dye electrodeposits were characterized by means of AFM, ESI-MS/MS and NMR spectroscopy. The obtained data reveal that the dye forms overlaid layers consisting of monomer molecules most likely with an antiparallel orientation...
May 10, 2018: Bioelectrochemistry
https://www.readbyqxmd.com/read/29753939/reactivation-of-standard-nife-hydrogenase-and-bioelectrochemical-catalysis-of-proton-reduction-and-hydrogen-oxidation-in-a-mediated-electron-transfer-system
#3
Saeko Shiraiwa, Keisei So, Yu Sugimoto, Yuki Kitazumi, Osamu Shirai, Koji Nishikawa, Yoshiki Higuchi, Kenji Kano
Standard [NiFe]-hydrogenase from Desulfovibrio vulgaris Miyazaki F (DvMF-H2 ase) catalyzes the uptake and production of hydrogen (H2 ) and is a promising biocatalyst for future energy devices. However, DvMF-H2 ase experiences oxidative inactivation under oxidative stress to generate Ni-A and Ni-B states. It takes a long time to reactivate the Ni-A state by chemical reduction, whereas the Ni-B state is quickly reactivated under reducing conditions. Oxidative inhibition limits the application of DvMF-H2 ase in practical devices...
May 5, 2018: Bioelectrochemistry
https://www.readbyqxmd.com/read/29753937/electricity-generation-and-microbial-communities-in-microbial-fuel-cell-powered-by-macroalgal-biomass
#4
Nannan Zhao, Yinan Jiang, Merlin Alvarado-Morales, Laura Treu, Irini Angelidaki, Yifeng Zhang
The potential of macroalgae Laminaria digitata as substrate for bioelectricity production was examined in a microbial fuel cell (MFC). A maximum voltage of 0.5 V was achieved without any lag time due to the high concentration of glucose and mannitol in the hydrolysate. Total chemical oxygen demand removal efficiency reached over 95% at the end of batch run. Glucose and mannitol were degraded through isobutryrate as intermediate. The 16S rRNA gene high throughout sequencing analysis of anodic biofilm revealed complex microbial composition dominated by Bacteroidetes (39...
May 5, 2018: Bioelectrochemistry
https://www.readbyqxmd.com/read/29753938/electrotrophic-activity-and-electrosynthetic-acetate-production-by-desulfobacterium-autotrophicum-hrm2
#5
Zehra Zaybak, Bruce E Logan, John M Pisciotta
Electroautotrophic microorganisms accept electrons from a cathode as source of reducing equivalents to drive CO2 fixation by poorly understood mechanisms. Acetogenic bacteria were the first group found to possess the capability for electroautotrophic metabolism in pure culture with associated electrosynthesis of acetate as primary metabolite. Identification of additional electrotrophic species can contribute to our understanding of this unusual form of metabolism. Here, bioelectrochemical techniques, chemical analysis and microscopy were used to determine electrotrophic metabolism of Desulfobacterium autotrophicum HRM2...
May 4, 2018: Bioelectrochemistry
https://www.readbyqxmd.com/read/29783192/interaction-of-dna-and-mononucleotides-with-theophylline-investigated-using-electrochemical-biosensors-and-biosensing
#6
Katarína Nemčeková, Ján Labuda, Viktor Milata, Jana Blaškovičová, Jozef Sochr
The understanding of DNA-drug interaction mechanism is among the important aspects of biological studies for drug design, discovery and pharmaceutical development processes. Published rather detailed FTIR and UV-visible spectroscopic studies on the interactions of theophylline, theobromine and caffeine with calf thymus DNA have shown effective binding of these methylxanthine derivatives to DNA and RNA involving H-bonds. However, to our knowledge, there is no such investigation using electrochemical approach...
May 3, 2018: Bioelectrochemistry
https://www.readbyqxmd.com/read/29742484/cost-effective-three-dimensional-ag-polymer-dyes-graphene-carbon-spheres-hybrids-for-high-performance-nonenzymatic-sensor-and-its-application-in-living-cell-h-2-o-2-detection
#7
Baoping Lu, Xuna Yuan, Yuehong Ren, Qinghua Shi, Song Wang, Jinlong Dong, Ze-Dong Nan
We describe a facile method to synthesize a new type of catalyst by electrodepositing Ag nanocrystals (AgNCs) on the different polymer dyes, Poly (methylene blue) (PMB) or Poly (4-(2-Pyridylazo)-Resorcinol) (PAR) modified graphene‑carbon spheres (GS) hybrids. The self-assembled GS take dual advantages of carbon spheres and graphene. Carbon spheres acts as nano-spacers prevent the aggregation of graphene and guarantee the fast electron transfer of GS. Secondly, polymerized dyes used here are beneficial for AgNCs growing as a linker...
May 3, 2018: Bioelectrochemistry
https://www.readbyqxmd.com/read/29747131/an-electrochemical-sensing-approach-for-scouting-microbial-chemolithotrophic-metabolisms
#8
Albert Saavedra, Federico Figueredo, Eduardo Cortón, Ximena C Abrevaya
The present study was aimed to test an electrochemical sensing approach for the detection of an active chemolithotrophic metabolism (and therefore the presence of chemolithotrophic microorganisms) by using the corrosion of pyrite by Acidithiobacillus ferrooxidans as a model. Different electrochemical techniques were combined with adhesion studies and scanning electron microscopy (SEM). The experiments were performed in presence or absence of A. ferrooxidans and without or with ferrous iron in the culture medium (0 and 0...
May 1, 2018: Bioelectrochemistry
https://www.readbyqxmd.com/read/29747129/electrochemical-behavior-of-pyrite-in-sulfuric-acid-in-presence-of-amino-acids-belonging-to-the-amino-acid-sequence-of-rusticyanin
#9
Biljana S Maluckov, Miodrag N Mitrić
The impact of different concentrations of three amino acids (cysteine, histidine and methionine) which are part of the amino acid sequence of rusticyanin on dissolution of pyrite is investigated by the application of electrochemical techniques. Cyclic voltammetric studies conducted in the anodic direction from corrosion potential have shown that in the vicinity of corrosion potential, histidine and methionine do not influence dissolution of pyrite independently on their concentrations. On the other hand, cysteine and solutions of these amino acids in the molar ratios Cys:His:Met/1:1:1 and Cys:His:Met/1:2:1 accelerate dissolution at concentrations 10-2  mol L-1 and 10-3  mol L-1 ...
May 1, 2018: Bioelectrochemistry
https://www.readbyqxmd.com/read/29729642/design-and-fabrication-of-an-electrochemical-aptasensor-using-au-nanoparticles-carbon-nanoparticles-cellulose-nanofibers-nanocomposite-for-rapid-and-sensitive-detection-of-staphylococcus-aureus
#10
Saba Ranjbar, Saeed Shahrokhian
Since that pathogenic bacteria are major threats to human health, this paper describes the fabrication of an effective and durable sensing platform based on gold nanoparticles/carbon nanoparticles/cellulose nanofibers nanocomposite (AuNPs/CNPs/CNFs) at the surface of glassy carbon electrode for sensitive and selective detection of Staphylococcus aureus (S. aureus). The AuNPs/CNPs/CNFs nanocomposite with the high surface area, excellent conductivity, and good biocompatibility was used for self-assembled of the thiolated specific S...
April 27, 2018: Bioelectrochemistry
https://www.readbyqxmd.com/read/29729643/nanosecond-bipolar-pulse-generators-for-bioelectrics
#11
Shu Xiao, Chunrong Zhou, Enbo Yang, Sambasiva R Rajulapati
Biological effects caused by a nanosecond pulse, such as cell membrane permeabilization, peripheral nerve excitation and cell blebbing, can be reduced or cancelled by applying another pulse of reversed polarity. Depending on the degree of cancellation, the pulse interval of these two pulses can be as long as dozens of microseconds. The cancellation effect diminishes as the pulse duration increases. To study the cancellation effect and potentially utilize it in electrotherapy, nanosecond bipolar pulse generators must be made available...
April 26, 2018: Bioelectrochemistry
https://www.readbyqxmd.com/read/29751230/the-anti-biofouling-behavior-of-high-voltage-pulse-electric-field-hpef-mediated-by-carbon-fiber-composite-coating-in-seawater
#12
Tiantian Feng, Jinyi Wu, Ke Chai, Pengpeng Yang
One of the most important research areas in the marine industry is to investigate new and effective anti-biofouling technologies. In this study, high voltage pulse electric field (HPEF) mediated by carbon fiber (CF) composite coating was utilized to prevent the fouling of bacteria, microalgae and barnacle larvae in seawater. The plate count, 2, 3, 5-triphenyl-tetrazolium chloride (TTC) reduction assay and neutral red (NR) staining and larval motility detection showed that the inactivation rates were at the highest levels, which reached 99...
April 25, 2018: Bioelectrochemistry
https://www.readbyqxmd.com/read/29729644/medium-s-conductivity-and-stage-of-growth-as-crucial-parameters-for-efficient-hydrocarbon-extraction-by-electric-field-from-colonial-micro-algae
#13
Alexis Guionet, Bahareh Hosseini, Hidenori Akiyama, Hamid Hosano
The green algae Botryococcus braunii produces a high amount of extracellular hydrocarbon, making it a promising algae in the field of bio-fuels production. As it mainly produces squalene like hydrocarbons, cosmetic industries are also interested in its milking. Pulsed electric fields (PEF) are an innovative method allowing oil extraction from micro-algae. In common algae accumulating hydrocarbon inside cytoplasm (Chlorella vulgaris, Nannochloropsis sp., etc), electric fields can destroy cell membranes, allowing the release of hydrocarbon...
April 25, 2018: Bioelectrochemistry
https://www.readbyqxmd.com/read/29734031/hydrogen-peroxide-biosensor-based-on-chitosan-2d-layered-double-hydroxide-composite-for-the-determination-of-h-2-o-2
#14
Jian Yuan, Sheng Xu, Hong-Yan Zeng, Xi Cao, A Dan Pan, Gao-Fei Xiao, Peng-Xuan Ding
The composites (LDH-CMC) composed of carboxymethyl chitosan (CMC) and 2D ZnAl layered double hydroxide (LDH) were successfully prepared using the one-step urea method; these composites were characterized by XRD, FT-IR, UV-vis DRS, SEM, BJH/BET, TG-DTG and pHzpc analyses, cyclic voltammetry, and electrochemical impedance spectroscopy. The use of CMC could impact the textural and surface chemical properties of the LDH-CMC composites, where the composites still maintained the 2D layered structure. Incorporating a moderate amount of CMC could increase both the surface area and the permanent charge density of the composites, leading to improved electrochemical performances...
April 23, 2018: Bioelectrochemistry
https://www.readbyqxmd.com/read/29723805/severe-microbiologically-influenced-corrosion-of-s32654-super-austenitic-stainless-steel-by-acid-producing-bacterium-acidithiobacillus-caldus-sm-1
#15
Yuqiao Dong, Baota Jiang, Dake Xu, Chengying Jiang, Qi Li, Tingyue Gu
Microbiologically influenced corrosion (MIC) of S32654 (654SMO) super austenitic stainless steel (SASS) by acid producing bacterium (APB), Acidithiobacillus caldus SM-1, a strain of sulfur-oxidizing bacteria (SOB) used in biohydrometallurgy field, was investigated using electrochemical measurements and surface characterizations during a 14-day immersion test. The results indicated that S32654 SASS was susceptible to MIC by APB, and A. caldus SM-1 was capable of producing an aggressive acidic environment underneath the biofilm, resulting in the dissolution of the passive film and severe pitting attacks against S32654 SASS, which is commonly regarded as a corrosion resistant material...
April 23, 2018: Bioelectrochemistry
https://www.readbyqxmd.com/read/29723806/bioelectrical-coupling-in-multicellular-domains-regulated-by-gap-junctions-a-conceptual-approach
#16
REVIEW
Javier Cervera, Alexis Pietak, Michael Levin, Salvador Mafe
We review the basic concepts involved in bioelectrically-coupled multicellular domains, focusing on the role of membrane potentials (Vmem ). In the first model, single-cell Vmem is modulated by two generic polarizing and depolarizing ion channels, while intercellular coupling is implemented via voltage-gated gap junctions. Biochemical and bioelectrical signals are integrated via a feedback loop between Vmem and the transcription and translation of a protein forming an ion channel. The effective rate constants depend on the single-cell Vmem because these potentials modulate the local concentrations of signaling molecules and ions...
April 21, 2018: Bioelectrochemistry
https://www.readbyqxmd.com/read/29747130/binder-materials-for-the-cathodes-applied-to-self-stratifying-membraneless-microbial-fuel-cell
#17
Xavier Alexis Walter, John Greenman, Ioannis Ieropoulos
The recently developed self-stratifying membraneless microbial fuel cell (SSM-MFC) has been shown as a promising concept for urine treatment. The first prototypes employed cathodes made of activated carbon (AC) and polytetrafluoroethylene (PTFE) mixture. Here, we explored the possibility to substitute PTFE with either polyvinyl-alcohol (PVA) or PlastiDip (CPD; i.e. synthetic rubber) as binder for AC-based cathode in SSM-MFC. Sintered activated carbon (SAC) was also tested due to its ease of manufacturing and the fact that no stainless steel collector is needed...
April 19, 2018: Bioelectrochemistry
https://www.readbyqxmd.com/read/29719274/a-novel-drug-free-strategy-of-nano-pulse-stimulation-sequence-npss-in-oral-cancer-therapy-in-vitro-and-in-vivo-study
#18
Jinsong Guo, Feihong Dong, Lian Ding, Kaile Wang, Jue Zhang, Jing Fang
Nano-pulse stimulation (NPS) is a novel technology to induce cancer apoptosis. In this study, based on the energy-dose effect of NPS, we designed a special NPS sequence (NPSS) with low field intensity. The effectiveness and mechanisms of NPSS on oral cancer therapy were evaluated by cell proliferation assays, microscopic investigation, JC-1 mitochondrial membrane potential assay, tumor inhibition assays, immunohistochemistry (IHC) assay, Ca2+ , NOS and ROS detection assays, respectively. The results demonstrated that NPSS treatment significantly inhibited oral cancer growth in vitro and in vivo...
April 19, 2018: Bioelectrochemistry
https://www.readbyqxmd.com/read/29715586/biological-effects-in-photodynamic-treatment-combined-with-electropermeabilization-in-wild-and-drug-resistant-breast-cancer-cells
#19
Joanna Weżgowiec, Julita Kulbacka, Jolanta Saczko, Joanna Rossowska, Grzegorz Chodaczek, Małgorzata Kotulska
Electrochemotherapy became one of the therapeutic protocols successfully used in oncology. However, biological effects occurring in cells, especially those which are drug resistant, have not been studied thoroughly. This study presents response of wild and drug resistant breast cancer cells to classical photodynamic therapy with Photofrin or experimental photodynamic therapy with cyanine IR-775, combined with electroporation. Photodynamic reaction or electroporation alone had no cytotoxic effect, but their combination significantly disturbed cellular functions...
April 19, 2018: Bioelectrochemistry
https://www.readbyqxmd.com/read/29715585/on-the-interaction-of-the-highly-charged-peptides-casocidins-with-biomimetic-membranes
#20
Lucia Becucci, Giovanni Aloisi, Andrea Scaloni, Simonetta Caira, Rolando Guidelli
Casocidin I and II (CI and CII) are structurally related antimicrobial peptides made of 39 and 31 amino acids, respectively, which derive from natural proteolytic processing of αs2 -casein and adopt an ordered α-helical structure in biomimetic membranes. Their putative membrane-permeabilizing activity was investigated at Hg-supported self-assembled monolayers (SAMs) and at tethered bilayer lipid membranes (tBLMs); the latter consisted of a monolayer of 2,3,di-O-phytanyl-sn-glycerol-1-tetraethylene-glycol-d,l-α lipoic acid ester thiolipid (DPTL), with a dioleoylphosphatidylcholine (DOPC) or dioleoylphosphatidylserine (DOPS) monolayer on top of it...
April 19, 2018: Bioelectrochemistry
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