journal
MENU ▼
Read by QxMD icon Read
search

American Journal of Physiology. Lung Cellular and Molecular Physiology

journal
https://www.readbyqxmd.com/read/28062487/alcohol-drives-s-nitrosylation-and-redox-activation-of-protein-phosphatase-1-causing-bovine-airway-cilia-dysfunction
#1
Michael Earl Price, Jacqueline A Pavlik, Miao Liu, Shi-Jian Ding, Todd A Wyatt, Joseph H Sisson
Individuals with alcohol (ethanol) use disorders are at increased risk for lung infections in part due to defective mucociliary clearance driven by motile cilia in the airways. We recently reported that isolated demembranated bovine cilia (axonemes) are capable of producing nitric oxide (∙NO) when exposed to biologically relevant concentrations of alcohol. This increased presence of ∙NO can lead to protein S-nitrosylation, a posttranslational modification signaling mechanism involving reversible adduction of nitrosium cations or ⋅NO to thiolate or thiyl groups, respectively, of proteins forming S-nitrosothiols...
January 6, 2017: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/28062486/plasma-membrane-wounding-and-repair-in-pulmonary-diseases
#2
Xiaofei Cong, Rolf Dieter Hubmayr, Changgong Li, Xiaoli Zhao
Various pathophysiological conditions such as surfactant dysfunction, mechanical ventilation, inflammation, pathogen products, environmental exposures and gastric acid aspiration stress lung cells and the compromise of plasma membranes occur as a result. The mechanisms necessary for cells to repair plasma membrane defects have been extensively investigated in the last two decades, and some of these key repair mechanisms are also shown to occur following lung cell injury. As it was theorized that lung wounding and repair are involved in the pathogenesis of acute respiratory distress syndrome (ARDS) and idiopathic pulmonary fibrosis (IPF), in this review, we summarized the experimental evidence of lung cell injury in these two devastating syndromes, discuss relevant genetic, physical and biological injury mechanisms, as well as mechanisms utilized by lung cells for cell survival and membrane repair...
January 6, 2017: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/28062485/activation-of-bitter-taste-receptors-in-pulmonary-nociceptors-sensitizes-trpv1-channels-through-plc-and-pkc-signaling-pathway
#3
Qihai Gu, Deanna S Joe, Carolyn A Gilbert
Bitter taste receptors (T2Rs), a G-protein-coupled receptor family capable of detecting numerous bitter-tasting compounds, have recently been shown to be expressed and play diverse roles in many extraoral tissues. Here we report the functional expression of T2Rs in rat pulmonary sensory neurons. In anesthetized spontaneously breathing rats, intratracheal instillation of T2Rs agonist chloroquine (10 mM, 0.1 ml) significantly augmented chemoreflexes evoked by right-atrial injection of capsaicin, a specific activator for transient receptor potential vanilloid receptor 1 (TRPV1); whereas intravenous infusion of chloroquine failed to significantly affect capsaicin-evoked reflexes...
January 6, 2017: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/28062484/heterogeneity-of-airway-dimensions-in-humans-a-critical-determinant-of-lung-function-in-asthmatics-and-non-asthmatics
#4
Christopher D Pascoe, Chun Y Seow, Tillie-Louise Hackett, Peter D Paré, Graham M Donovan
Airway remodeling, a key feature of asthma, alters every layer of the airway wall but most strikingly the airway smooth muscle (ASM) layer. Airway remodeling in asthmatics contributes to fixed airflow obstruction and can amplify airway narrowing caused by ASM activation. Previous modeling studies have shown that the increase in ASM mass has the largest effect on increasing maximal airway narrowing. Simulated heterogeneity in the dimensions and properties of the airway wall can further amplify airway narrowing...
January 6, 2017: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/28062483/the-sialic-acid-to-urea-ratio-as-a-measure-of-airway-surface-hydration
#5
Charles R Esther, David B Hill, Brian Button, Shuai Shi, Corey M Jania, Elizabeth A Duncan, Claire M Doerschuk, Gang Chen, Sarath Ranganathan, Stephen Michael Stick, Richard C Boucher
INTRODUCTION: Although airway mucus dehydration is key to pathophysiology of cystic fibrosis (CF) and other airways diseases, measuring mucus hydration is challenging. We explored a robust method to estimate mucus hydration using sialic acid as a marker for mucin content. METHODS: Terminal sialic acid residues from mucins were cleaved by acid hydrolysis from airway samples, and concentrations of sialic acid, urea, and other biomarkers analyzed using mass spectrometry...
January 6, 2017: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/28062482/dysregulated-nox4-ubiquitination-contributes-to-redox-imbalance-and-age-related-severity-of-acute-lung-injury
#6
Sunmi Palumbo, Yoon-Joo Shin, Kareem Ahmad, Ankit A Desai, Hector Quijada, Mohamed Mohamed, Adam Knox, Saad Sammani, Brett A Colson, Ting Wang, Joe G N Garcia, Louise Hecker
Acute respiratory distress syndrome (ARDS) is a devastating critical illness disproportionately affecting the elderly population(higher incidence and mortality). The integrity of the lung endothelial cell (EC) monolayer is critical for preservation of lung function. However, mechanisms mediating EC barrier regulation in aging remain unclear. We assessed the severity of acute lung injury (ALI) in young (2 months) and aged (18 months) mice using a two-hit pre-clinical model. Compared to young cohorts, aged mice exhibited increased ALI severity, with greater vascular permeability characterized by elevated albumin influx, levels of bronchoalveolar lavage (BAL) cells (neutrophils) and protein...
January 6, 2017: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/28062481/sex-specific-effects-of-sex-steroids-on-alveolar-epithelial-na-transport
#7
Melanie Haase, Mandy Laube, Ulrich H Thome
Alveolar fluid clearance mediates perinatal lung transition to air breathing in newborn infants, which is accomplished by epithelial Na+ channels (ENaC) and the Na,K-ATPase. Male sex represents a major risk factor for developing respiratory distress, especially in preterm infants. We previously showed that male sex is associated with reduced epithelial Na+ transport, possibly contributing to the sexual dimorphism in newborn respiratory distress. This study aimed to determine sex-specific effects of sex steroids on epithelial Na+ transport...
January 6, 2017: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/28039105/long-acting-%C3%AE-2-agonists-promote-glucocorticoid-mediated-repression-of-nf-%C3%AE%C2%BAb-by-enhancing-expression-of-the-feedback-regulator-tnfaip3
#8
Mohammed Omar Altonsy, Mahmoud M Mostafa, Anthony N Gerber, Robert Newton
Glucocorticoids, or corticosteroids, are effective treatments for many chronic inflammatory diseases and, in mild/moderate asthma, long-acting β2-adrenoceptor agonists (LABAs) enhance the efficacy of inhaled corticosteroids (ICSs) more than increasing the ICS dose. In human bronchial epithelial, BEAS-2B, cells, expression of TNFα-induced protein 3 (TNFAIP3), or A20, a dual-ubiquitin ligase that provides feedback inhibition of NF-κB, was induced by budesonide, an ICS, formoterol, a LABA, and was further enhanced by budesonide/formoterol combination...
December 29, 2016: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/28011618/novel-applications-for-a-non-invasive-sampling-method-of-the-nasal-mucosa
#9
Meghan E Rebuli, Adam M Speen, Phillip W Clapp, Ilona Jaspers
Reliable methods for sampling the nasal mucosa provide clinical researchers with key information regarding respiratory biomarkers of exposure and disease. For quick and non-invasive sampling of the nasal mucosa, nasal lavage (NL) collection has been widely used as a clinical tool, however, limitations including volume variability, sample dilution, and storage prevent NL collection from being used in non-laboratory settings and in the analysis of low abundance biomarkers. In this study, we optimize and validate a novel methodology using absorbent Leukosorb® paper cut to fit the nasal passage in order to extract epithelial lining fluid (ELF) from the nasal mucosa...
December 23, 2016: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/28011617/a-keratan-sulfate-disaccharide-prevents-inflammation-and-the-progression-of-emphysema-in-murine-models
#10
Congxiao Gao, Reiko Fujinawa, Takayuki Yoshida, Manabu Ueno, Fumi Ota, Yasuhiko Kizuka, Tetsuya Hirayama, Hiroaki Korekane, Shinobu Kitazume, Toshitaka Maeno, Kazuaki Ohtsubo, Keiichi Yoshida, Yoshiki Yamaguchi, Bernd Lepenies, Jonas Aretz, Christoph Rademacher, Hiroki Kabata, Ahmed E Hegab, Peter H Seeberger, Tomoko Betsuyaku, Kozui Kida, Naoyuki Taniguchi
Emphysema is a typical component of chronic obstructive pulmonary disease (COPD), a progressive and inflammatory airway disease. However, no effective treatment currently exists. Here we show that keratan sulfate (KS), one of the major glycosaminoglycans (GAGs) produced in the small airway, decreased in lungs of cigarette-smoke-exposed mice. In order to confirm the protective effect of KS in the small airway, a disaccharide repeating unit of KS designated L4 ([SO3--6]Galβ1-4[SO3--6]GlcNAc) was administered to two murine models: elastase-induced-emphysema and LPS-induced exacerbation of a cigarette smoke-induced emphysema...
December 23, 2016: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/28011616/targeted-epigenetic-editing-of-spdef-reduces-mucus-production-in-lung-epithelial-cells
#11
Juan Song, David Cano Rodriguez, Melanie Winkle, Rutger A F Gjaltema, Désirée Goubert, Tomasz P Jurkowski, Irene H Heijink, Marianne G Rots, Machteld N Hylkema
Airway mucus hypersecretion contributes to the morbidity and mortality in patients with chronic inflammatory lung diseases. Reducing mucus production is crucial for improving patients' quality of life. The transcription factor SAM-pointed domain-containing Ets-like factor (SPDEF) plays a critical role in the regulation of mucus production, and therefore represents a potential therapeutic target. This study aims to reduce lung epithelial mucus production by targeted silencing SPDEF using the novel strategy epigenetic editing...
December 23, 2016: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/27979861/bmi-1-extends-proliferative-potential-of-human-bronchial-epithelial-cells-whilst-retaining-their-mucociliary-differentiation-capacity
#12
Mustafa Mohamed Munye, Amelia Shoemark, Robert A Hirst, Juliette M Delhove, Tyson V Sharp, Tristan R McKay, Chistopher O'Callaghan, Deborah L Baines, Steven J Howe, Stephen L Hart
Air-liquid interface (ALI) culture of primary airway epithelial cells enables mucociliary differentiation providing an in vitro model of the human airway but their proliferative potential is limited. To extend proliferation, these cells were previously transduced with viral oncogenes or mouse Bmi-1 + hTERT but the resultant cell lines did not undergo mucociliary differentiation. We hypothesised that use of human BMI-1 alone would increase the proliferative potential of bronchial epithelial cells while retaining their mucociliary differentiation potential...
December 15, 2016: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/27979860/alternative-hematological-and-vascular-adaptive-responses-to-high-altitude-hypoxia-in-east-african-highlanders
#13
Hoi I Cheong, Allison J Janocha, Lawrence T Monocello, Adrianna C Garchar, Amha Gebremedhin, Serpil C Erzurum, Cynthia M Beall
Elevation of hemoglobin concentration, a common adaptive response to high-altitude hypoxia, occurs among Oromo, but is dampened among Amhara highlanders of East Africa. We hypothesized that Amhara highlanders offset their smaller hemoglobin response with a vascular response. We tested this by comparing Amhara and Oromo highlanders at 3700 m and 4000 m to their lowland counterparts at 1200 m and 1700 m. To evaluate vascular responses, we assessed urinary levels of nitrate (NO3(-)) as a readout of production of the vasodilator nitric oxide and its downstream signal transducer cyclic guanosine monophosphate (cGMP), along with diastolic blood pressure as an indicator of vasomotor tone...
December 15, 2016: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/27979859/capsaicin-induced-ca2-signaling-is-enhanced-via-upregulated-trpv1-channels-in-pulmonary-artery-smooth-muscle-cells-from-patients-with-idiopathic-pah
#14
Shanshan Song, Ramon J Ayon, Aya Yamamura, Hisao Yamamura, Swetaleena Dash, Aleksandra Babicheva, Haiyang Tang, Xutong Sun, Arlette G Cordery, Zain I Khalpey, Stephen M Black, Ankit A Desai, Franz Rischard, Kimberly M McDermott, Joe G N Garcia, Ayako Makino, Jason X-J Yuan
Capsaicin is an active component of chili pepper and a pain relief drug. Capsaicin can activate transient receptor potential vanilloid 1 (TRPV1) channels to increase cytosolic Ca2+ concentration ([Ca2+]cyt). A rise in [Ca2+]cyt in pulmonary artery smooth muscle cells (PASMCs) is an important stimulus for pulmonary vasoconstriction and vascular remodeling. In this study, we observed that capsaicin-induced increase in [Ca2+]cyt was significantly enhanced in PASMCs from patients with idiopathic pulmonary arterial hypertension (IPAH) compared with normal PASMCs...
December 15, 2016: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/27979858/mir-34a-promotes-fibrosis-in-aged-lungs-by-inducing-alveolar-epithelial-dysfunctions
#15
Huachun Cui, Jing Ge, Na Xie, Sami Banerjee, Yong Zhou, Rui-Ming Liu, Victor J Thannickal, Gang Liu
Idiopathic pulmonary fibrosis (IPF) is a well-known age-related disease. However, much less recognized has been the aging associated pathogenesis of this disorder. As we and others previously showed that dysregulation of microRNAs (miRNAs) was an important mechanism involved in pulmonary fibrosis, the role of these molecules in this pathology in the aged population has not been investigated. In this study, by using lung fibrosis model established in old mice, we found that ablation of miR-34a protected aged animals from developing experimental lung fibrosis...
December 15, 2016: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/27979857/endothelial-cell-signaling-and-ventilator-induced-lung-injury-vili-molecular-mechanisms-genomic-analyses-therapeutic-targets
#16
Ting Wang, Christine Gross, Ankit Desai, Evgeny Zemskov, Xiaomin Wu, Alexander N Garcia, Jeffrey R Jacobson, Jason X-J Yuan, Joe G N Garcia, Stephen M Black
Mechanical ventilation is a life-saving intervention in critically ill patients with respiratory failure due to acute respiratory distress syndrome (ARDS). Paradoxically, mechanical ventilation also creates excessive mechanical stress that directly augments lung injury, a syndrome known as ventilator-induced lung injury (VILI). The pathobiology of VILI and ARDS shares many inflammatory features including increases in lung vascular permeability due to loss of endothelial cell barrier integrity resulting in alveolar flooding...
December 15, 2016: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/27941077/post-translational-modification-of-%C3%AE-catenin-is-associated-with-pathogenic-fibroblastic-changes-in-bronchopulmonary-dysplasia
#17
Jennifer M S Sucre, Preethi Vijayaraj, Cody Aros, Dan Wilkinson, Manash Paul, Bruce Dunn, Susan H Guttentag, Brigitte N Gomperts
Bronchopulmonary dysplasia (BPD) is a common complication of premature birth. The histopathology of BPD is characterized by an arrest of alveolarization with fibroblast activation. The Wnt/β-catenin signaling pathway is important in early lung development. When Wnt signaling is active, phosphorylation of β-catenin by tyrosine kinases at activating sites, specifically at tyrosine 489 (Y489), correlates with nuclear localization of β-catenin. We examined fetal lung tissue, lung tissue from term newborns, and lung tissue from infants who died with BPD, and found nuclear β-catenin phosphorylatation at Y489 in epithelial and mesenchymal cells in fetal tissue and BPD tissue, but not the lungs of term infants...
December 9, 2016: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/27941076/airway-smooth-muscle-tone-increases-airway-responsiveness-in-healthy-young-adults
#18
Morgan Gazzola, Katherine Lortie, Cyndi Henry, Samuel Mailhot-Larouche, David G Chapman, Christian Couture, Chun Y Seow, Peter D Paré, Gregory G King, Louis-Philippe Boulet, Ynuk Bossé
Force adaptation, a process whereby sustained spasmogenic activation (viz., tone) of airway smooth muscle (ASM) increases its contractile capacity, has been reported in isolated ASM tissues in vitro, as well as in mice in vivo. The objective of the present study was to assess the effect of tone on airway responsiveness in humans. Ten healthy volunteers underwent methacholine challenge on two occasions. One challenge consisted of six serial doses of saline followed by a single high dose of methacholine. The other consisted of six low doses of methacholine five minutes apart followed by a higher dose...
December 9, 2016: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/27941075/%C3%AE-%C3%AE-%C3%AE-enac-is-inhibited-by-cftr-but-stimulated-by-camp-in-xenopus-laevis-oocytes
#19
Robert Rauh, Christian Hoerner, Christoph Korbmacher
The epithelial sodium channel (ENaC) and the CFTR chloride channel critically regulate airway surface liquid by driving fluid absorption and secretion, respectively. Their functional interplay is complex and incompletely understood. ENaC is a heteromeric channel with three well characterised subunits (α, β, γ). In humans, an additional δENaC subunit exists in lung and several other tissues where it may replace the α-subunit to form δβγENaC. Little is known about the physiological role of δβγENaC and its possible interaction with CFTR...
December 9, 2016: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/27941074/cartilage-rings-contribute-to-the-proper-embryonic-tracheal-epithelial-differentiation-metabolism-and-expression-of-inflammatory-genes
#20
Gianluca Turcatel, Katelyn Millette, Matthew Thornton, Stephanie Leguizamon, Brendan Grubbs, Wei Shi, David Warburton
The signaling crosstalk between the tracheal mesenchyme and epithelium has not been researched extensively leaving a substantial gap of knowledge in the mechanisms dictating embryonic development of the proximal airways by the adjacent mesenchyme. Recently, we have reported that embryos lacking mesenchymal expression of Sox9 did not develop tracheal cartilage rings and showed altered differentiation of the tracheal epithelium. Herein, we propose that tracheal cartilage provides local inductive signals responsible for the proper differentiation, metabolism, and inflammatory status regulation of the tracheal epithelium...
December 9, 2016: American Journal of Physiology. Lung Cellular and Molecular Physiology
journal
journal
34862
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"