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American Journal of Physiology. Lung Cellular and Molecular Physiology

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https://www.readbyqxmd.com/read/30407867/high-mobility-group-box-1-protein-from-cc10-club-cells-promotes-type-2-response-in-the-later-stage-of-respiratory-syncytial-virus-infection
#1
Sisi Chen, Guangyuan Yu, Jun Xie, Wei Tang, Leiqiong Gao, Xiaoru Long, Luo Ren, Xiaohong Xie, Yu Deng, Zhou Fu, Enmei Liu
The type 2 immune response, induced by infection of respiratory syncytial virus (RSV), has been linked to asthma development, but it remains unclear how the response is initiated. Here, we reported that the high mobility group box-1 (HMGB1) protein promotes the type 2 response in the later stage of RSV infection. In mice, we found that type 2 cytokines were elevated in the later stages, which were strongly diminished after administration of anti-HMGB1 antibodies. Further investigation revealed that HMGB1 expression was localized to CC10+ club cells in the lung...
November 8, 2018: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/30407866/short-chain-fatty-acids-increase-tnf%C3%AE-induced-inflammation-in-primary-human-lung-mesenchymal-cells-through-the-activation-of-p38-map-kinase
#2
Sandra Rutting, Dia Xenaki, Monique Malouf, Jay C Horvat, Lisa G Wood, Philip M Hansbro, Brian G Oliver
Short chain fatty acids (SCFAs), produced as by-products of dietary fibre metabolism by gut bacteria, have anti-inflammatory properties and could potentially be used for the treatment of inflammatory diseases, including asthma. The direct effects of SCFAs on inflammatory responses in primary human lung mesenchymal cells have not been assessed. We investigated whether SCFAs can protect against TNFα-induced inflammation in primary human lung fibroblasts (HLFs) and airway smooth muscle (ASM) cells in vitro. HLFs and ASM cells were exposed to SCFAs, acetate (C2:0), propionate (C3:0) and butyrate (C4:0) (0...
November 8, 2018: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/30407865/hdac2-attenuates-airway-inflammation-by-suppressing-il17a-production-in-hdm-challenged-mice
#3
Tianwen Lai, Mindan Wu, Chao Zhang, Luanqing Che, Feng Xu, Yong Wang, Yanping Wu, Nanxia Xuan, Chao Cao, Xufei Du, Bin Wu, Wen Li, Songmin Ying, Huahao Shen, Zhihua Chen
Histone deacetylase (HDAC) 2 is expressed in airway epithelium and plays a pivotal role in inflammatory cells. However, the role of HDAC2 in allergic airway inflammation remains poorly unknown. In the present study, we determined the role of HDAC2 in airway inflammation using in vivo models of HDM-induced allergic inflammation and in vitro cultures of human bronchial epithelial (HBE) cells exposed to HDM, IL17A, or both. We observed that HDM-challenged Hdac2+/- mice exhibited substantially enhanced infiltration of inflammatory cells...
November 8, 2018: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/30407864/could-nanotechnology-make-vitamin-e-therapeutically-effective
#4
Tetsuya Tamura, Gail Otulakowski, Brian P Kavanagh
Vitamin E (VitE) has important antioxidant and anti-inflammatory effects and is necessary for normal physiological fuction. α-Tocopherol (α-T), the predominant form of VitE in human tissues, has been extensively studied, yet therapeutic trials of VitE have been uniformly negative. We postulate that a nanoparticle approach might provide effective delivery and therapeutic effect.
November 8, 2018: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/30407863/attenuation-of-murine-and-human-airway-contraction-by-a-peptide-fragment-of-the-cytoskeleton-regulatory-protein-gelsolin
#5
Maya Mikami, Jose F Perez-Zoghbi, Yi Zhang, Charles W Emala
We have previously reported that mice genetically deficient in the actin binding protein gelsolin exhibit impaired airway smooth muscle (ASM) relaxation. Primary cultured ASM cells from these mice demonstrate enhanced inositol triphosphate (IP3 ) synthesis and increased intracellular calcium in response to Gq-coupled agonists. We hypothesized that this was due to increased intracellular availability of unbound phosphatidylinositol 4,5-bisphosphate (PIP2 ), based on the fact that gelsolin contains a short peptide region that binds PIP2 , presumably making it a less available substrate...
November 8, 2018: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/30407862/sex-specific-airway-hyperreactivity-and-sex-specific-transcriptome-remodeling-in-neonatal-piglets-challenged-with-intra-airway-acid
#6
Leah R Reznikov, Yan-Shin J Liao, Tongjun Gu, Katelyn M Davis, Shin-Ping Kuan, Kalina R Atanasova, Joshua S Dadural, Emily N Collins, Maria V Guevara, Kevin Vogt
Acute airway acidification is a potent stimulus of sensory nerves and occurs commonly with gastroesophageal reflux disease, cystic fibrosis and asthma. In infants and adults, airway acidification can acutely precipitate asthma-like symptoms, and treatment resistant asthma can be associated with gastroesophageal reflux disease. Airway protective behaviors, such as mucus secretion and airway smooth muscle contraction, are often exaggerated in asthma. These behaviors are manifested through activation of neural circuits...
November 8, 2018: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/30382768/epithelial-serpinb10-a-novel-marker-of-airway-eosinophilia-in-asthma-contributes-to-allergic-airway-inflammation
#7
Yuqing Mo, Kan Zhang, Yuchen Feng, Lingling Yi, Yuxia Liang, Wenliang Wu, Jian-Ping Zhao, Zhenxiang Zhang, Yongjian Xu, Qinghua Hu, Jianguo He, Guohua Zhen
Serine peptidase inhibitor, clade B, member 10 (SERPINB10) expression is increased in IL-13-stimulated human bronchial epithelial cells and in a murine model of allergic airway inflammation. However, the role of SERPINB10 in asthma remains unknown. We examined the association between epithelial SERPINB10 expression and airway eosinophilia in subjects with asthma and the role of Serpinb10 in allergic airway inflammation in animal model. Epithelial SERPINB10 mRNA and protein expression were markedly increased in subjects with asthma ( n = 60) compared with healthy controls ( n = 25)...
November 1, 2018: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/30382767/early-disruption-of-the-alveolar-capillary-barrier-in-a-ricin-induced-ards-mouse-model-neutrophil-dependent-and-independent-impairment-of-junction-proteins
#8
Anita Sapoznikov, Yoav Gal, Reut Falach, Irit Sagi, Sharon Ehrlich, Elad Lerer, Arik Makovitzki, Anna Aloshin, Chanoch Kronman, Tamar Sabo
Irrespective of its diverse etiologies, ALI/ARDS leads to increased permeability of the alveolar-capillary barrier, which in turn, promotes edema formation and respiratory failure. We investigated the mechanism of ALI/ARDS lung hyperpermeability triggered by pulmonary exposure of mice to the highly toxic plant-derived toxin ricin. One prominent hallmark of ricin-mediated pulmonary intoxication is the rapid and massive influx of neutrophils to the lungs, where they contribute to the developing inflammation yet may also cause tissue damage, thereby promoting ricin-mediated morbidity...
November 1, 2018: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/30382766/microrna-30a-as-a-candidate-underlying-sex-specific-differences-in-neonatal-hyperoxic-lung-injury-implications-for-bpd
#9
Yuhao Zhang, Cristian Coarfa, Xiaoyu Dong, Weiwu Jiang, Brielle Hayward-Piatkovskyi, Jason P Gleghorn, Krithika Lingappan
Premature male neonates are at a greater risk of developing bronchopulmonary dysplasia (BPD). The reasons underlying sexually dimorphic outcomes in premature neonates are not known. The role of miRNAs in mediating sex biases in BPD is understudied. Analysis of the pulmonary transcriptome revealed that a large percentage of angiogenesis-related differentially expressed genes are miR-30a targets. We tested the hypothesis that there is differential expression of miR-30a in vivo and in vitro in neonatal human pulmonary vascular endothelial cells (HPMEC) upon exposure to hyperoxia...
November 1, 2018: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/30358443/inflammation-induced-upregulation-of-p2x-4-expression-augments-mucin-secretion-in-airway-epithelia
#10
Veronika E Winkelmann, Kristin E Thompson, Kathrin Neuland, Ana-Maria Jaramillo, Giorgio Fois, Hanna Schmidt, Oliver H Wittekindt, Wei Han, Michael J Tuvim, Burton F Dickey, Paul Dietl, Manfred Frick
Mucus clearance provides an essential innate defense mechanism to keep the airways and lungs free of particles and pathogens. Baseline and stimulated mucin secretion from secretory airway epithelial cells need to be tightly regulated to prevent mucus hypersecretion and mucus plugging of the airways. It is well established that extracellular ATP is a potent stimulus for regulated mucus secretion. Previous studies revealed that ATP acts via metabotropic P2Y2 purinoreceptors on goblet cells. Extracellular ATP, however, is also a potent agonist for ionotropic P2X purinoreceptors...
October 25, 2018: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/30358442/theophylline-inhibits-cigarette-smoke-induced-inflammation-in-skeletal-muscle-by-upregulating-hdac2-expression-and-decreasing-nf-%C3%AE%C2%BAb-activation
#11
Yanfei Bin, Ying Xiao, Dongmei Huang, Zhiying Ma, Yi Liang, Jing Bai, Wenlu Zhang, Qiuli Liang, Jian-Quan Zhang, Xiaoning Zhong, Zhi-Yi He
Inflammation is associated with skeletal muscle dysfunction and atrophy in patients with chronic obstructive pulmonary disease (COPD). Theophylline has an anti-inflammatory role in COPD. However, the effects of theophylline on inflammation in skeletal muscle in COPD have rarely been reported. The aims of this study were to explore whether theophylline has an anti-inflammatory effect on skeletal muscle in a mouse model of emphysema and to investigate the molecular mechanism underlying this effect. In mice, cigarette smoke (CS) exposure for 28 weeks resulted in atrophy of the gastrocnemius muscle...
October 25, 2018: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/30358441/kininogen-deficiency-or-depletion-reduces-enhanced-pause-independent-of-pulmonary-inflammation-in-a-house-dust-mite-induced-murine-asthma-model
#12
Jack Yang, Cornelis van 't Veer, Joris J T H Roelofs, Jeroen W van Heijst, Alex F de Vos, Keith R McCrae, Alexey Revenko, Jeff R Crosby, Tom van der Poll
BACKGROUND: High molecular weight kininogen is an important substrate of the kallikrein-kinin system. Activation of this system has been associated with aggravation of hallmark features in asthma. OBJECTIVES: We aimed to determine the role of kininogen in enhanced pause (Penh) measurements and lung inflammation in a house dust mite (HDM) induced murine asthma model. METHODS: Normal wild-type mice and mice with a genetic deficiency of kininogen were subjected to repeated HDM exposure (sensitization on days 0,1 and 2; challenge on days 14, 15, 18 and 19) via the airways to induce allergic lung inflammation...
October 25, 2018: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/30358440/-cyclodextrins-reduce-the-ability-of-pseudomonas-outer-membrane-vesicles-to-reduce-cftr-cl-secretion
#13
Roxanna Barnaby, Katja Koeppen, Bruce A Stanton
Pseudomonas aeruginosa secretes outer membrane vesicles (OMVs) that fuse with cholesterol-rich lipid rafts in the apical membrane of airway epithelial cells and decrease wt-CFTR Cl- secretion. Herein, we tested the hypothesis that a reduction of the cholesterol content of CF human airway epithelial cells by cyclodextrins reduces the inhibitory effect of OMVs on VX-809-stimulated Phe508del CFTR Cl- secretion. Primary CF bronchial epithelial cells, and CFBE cells were treated with vehicle, hydroxypropyl-beta-cyclodextrin (HPbCD) or methyl-beta-cyclodextrin (MbCD) and the effects of OMVs secreted by Pseudomonas aeruginosa on VX-809 stimulated Phe508del CFTR Cl- secretion were measured in Ussing chambers...
October 25, 2018: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/30358439/hdac8-inhibition-ameliorates-pulmonary-fibrosis
#14
Shigeki Saito, Yan Zhuang, Takayoshi Suzuki, Yosuke Ota, Marjorie E Bateman, Ala L Alkhatib, Gilbert Franklin Morris, Joseph A Lasky
Idiopathic pulmonary fibrosis (IPF) is a fibroproliferative lung disease, and fibroblast-myofibroblast differentiation (FMD) is thought to be a key event in the pathogenesis of IPF. Histone deacetylase 8 (HDAC8) has been shown to associate with α-smooth muscle actin (α-SMA; a marker of FMD) and regulates cell contractility in vascular smooth muscle cells. However, the role of HDAC8 in FMD or pulmonary fibrosis has never been reported. This study investigated the role of HDAC8 in pulmonary fibrosis, with a focus on FMD...
October 25, 2018: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/30358438/myd88-controls-airway-epithelial-muc5ac-expression-during-tlr-activation-conditions-from-agricultural-organic-dust-exposure
#15
John D Dickinson, Jenea M Sweeter, Elizabeth B Staab, Amy J Nelson, Kristina L Bailey, Kristi Warren, Ana-Maria Jaramillo, Burton F Dickey, Jill A Poole
Inflammation from airborne microbes can overwhelm compensatory mucociliary clearance mechanisms leading to mucous cell metaplasia. Toll-like receptor activation (TLR) via MyD88 signaling is central to pathogen responses. We have previously shown that agricultural organic dust extract (ODE), with abundant microbial component diversity, activates TLR-induced airway inflammation. Using an established model, C57BL/6J wild type (WT) and global MyD88 knock-out (KO) mice were treated with intranasal inhalation of ODE or saline daily for 1 week...
October 25, 2018: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/30358437/acute-pulmonary-effects-of-aerosolized-nicotine
#16
Shama Ahmad, Iram Zafar, Nithya Mariappan, Maroof Husain, Chih-Chang Wei, Nilam Vetal, Isam A Eltoum, Aftab Ahmad
Nicotine is a highly addictive principal component of both tobacco and electronic cigarette that is readily absorbed in blood. Nicotine containing electronic cigarettes are promoted as a safe alternative to cigarette smoking. However, the isolated effects of inhaled nicotine are largely unknown. Here we report a novel rat model of aerosolized nicotine with a particle size (~1 micron) in the respirable diameter range. Acute nicotine inhalation caused increased pulmonary edema and lung injury as measured by enhanced bronchoalveolar lavage fluid (BALF) protein, IgM, lung wet-to-dry weight ratio and high mobility group box 1 (HMGB1) protein and decreased lung E-cadherin protein...
October 25, 2018: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/30358436/divergent-changes-of-p53-in-pulmonary-arterial-endothelial-and-smooth-muscle-cells-involved-in-the-development-of-pulmonary-hypertension
#17
Ziyi Wang, Kai Yang, Qiuyu Zheng, Chenting Zhang, Haiyang Tang, Aleksandra Babicheva, Qian Jiang, Meichan Li, Yuqin Chen, Shane G Carr, Kang Wu, Qian Zhang, Angela Balistrieri, Christina Wang, Shanshan Song, Ramon J Ayon, Ankit A Desai, Stephen M Black, Joe G N Garcia, Ayako Makino, Jason X-J Yuan, Wenju Lu, Jian Wang
The tumor-suppressive role of p53, a transcription factor that regulates the expression of many genes, has been linked to cell cycle arrest, apoptosis, andsenescence. The non-canonical function or the pathogenic role of p53 has more recently been implicatedin pulmonary vascular disease.We previously reported that rapid nuclear accumulation of hypoxia-inducible factor (HIF)-1α in pulmonary arterial smooth muscle cells (PASMCs) upregulates TRPC channels and enhances Ca2+ entry to increase cytosolic Ca2+ concentration ([Ca2+ ]cyt )...
October 25, 2018: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/30358435/cholesterol-modulates-signaling-of-the-chemosensory-bitter-taste-receptor-t2r14-in-human-airway-cells
#18
Feroz Ahmed Shaik, Manoj Reddy Medapati, Prashen Chelikani
Bitter taste receptors (T2Rs) are a group of 25 chemosensory receptors expressed at significant levels in the human airways. In human airways bitter taste receptor 14 (T2R14) mediated physiological response in ameliorating obstructive airway disorders is an active area of investigation. Therefore, understanding various factors regulating the structure and function of T2R14 will be beneficial. We hypothesize that membrane lipids like cholesterol play a regulatory role in T2R14 signaling in airway cells. We confirmed the expression and signaling of T2R14 in primary human airway smooth muscle (HASM) cells, and human airway epithelial cell line (NuLi-1) using immunoblot analysis and [Ca2+ ]i mobilization experiments respectively...
October 25, 2018: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/30335500/characterization-of-human-pdgfr%C3%AE-positive-pericytes-from-ipf-and-non-ipf-lungs
#19
Carole L Wilson, Sarah E Stephenson, Jean Paul Higuero, Carol Feghali-Bostwick, Chi F Hung, Lynn M Schnapp
Pericytes are key regulators of the microvasculature through their close interactions with the endothelium. However, pericytes play additional roles in tissue homeostasis and repair, in part by transitioning into myofibroblasts. Accumulation of myofibroblasts is a hallmark of fibrotic diseases such as idiopathic pulmonary fibrosis (IPF). To understand the contribution and role of pericytes in human lung fibrosis, we isolated these cells from non-IPF control and IPF lung tissues based on expression of PDGFRβ, a common marker of pericytes...
October 18, 2018: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/30335499/protease-activated-receptor-2-signaling-through-%C3%AE-arrestin-2-mediates-alternaria-alkaline-serine-protease-induced-airway-inflammation
#20
Michael C Yee, Heddie L Nichols, Danny Polley, Mahmoud Saifeddine, Kasturi Pal, Kyu Lee, Emma H Wilson, Michael O Daines, Morley D Hollenberg, Scott Boitano, Kathryn A DeFea
Alternaria alternata is a fungal allergen associated with severe asthma and asthma exacerbations. Similar to other asthma-associated allergens, Alternaria secretes a serine-like trypsin protease(s) that is thought to act through the G protein-coupled receptor, protease-activated receptor-2 (PAR2 ), to induce asthma symptoms. However, specific mechanisms underlying Alternaria-induced PAR2 activation and signaling remain ill-defined. We sought to determine whether Alternaria-induced PAR2 signaling contributed to asthma symptoms via a PAR2 /β-arrestin signaling axis, to identify the protease activity responsible for PAR2 signaling and determine if protease activity was sufficient for Alternaria-induced asthma symptoms in animal models...
October 18, 2018: American Journal of Physiology. Lung Cellular and Molecular Physiology
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