journal
MENU ▼
Read by QxMD icon Read
search

American Journal of Physiology. Cell Physiology

journal
https://www.readbyqxmd.com/read/27903587/changes-in-mitochondrial-morphology-and-organisation-can-enhance-energy-supply-from-mitochondrial-oxidative-phosphorylation-in-diabetic-cardiomyopathy
#1
Jan Jarosz, Shouryadipta Ghosh, Lea M D Delbridge, Amorita Volschenk, Anthony Jr Hickey, Edmund J Crampin, Eric Hanssen, Vijay Rajagopal
Diabetic cardiomyopathy is accompanied by metabolic and ultrastructural alterations, but the impact of the structural changes on metabolism itself is yet to be determined. Morphometric analysis of mitochondrial shape and spatial organisation within transverse sections of cardiomyocytes from control and streptozotocin induced type I diabetic Sprague-Dawley rats revealed that mitochondria are 20% smaller in size while their spatial density increases by 53% in diabetic cells relative to control myocytes. Diabetic cells formed larger clusters of mitochondria (60% more mitochondria per cluster) and the effective surface to volume ratio of these clusters increased by 22...
November 30, 2016: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/27903586/microrna-125a-5p-alleviates-the-deleterious-effects-of-ox-ldl-on-multiple-functions-of-human-brain-microvessel-endothelial-cells
#2
Qunwen Pan, Xiaorong Liao, Hua Liu, Yan Wang, Yanfang Chen, Bin Zhao, Eric Lazartigues, Yi Yang, Xiaotang Ma
MicroRNA-125a-5p(miR-125a-5p)could participate in the pathogenesis of vascular diseases. In this study, we investigated the role of miR-125a-5p in oxidized low density lipoprotein (ox-LDL) induced functional changes in human brain microvessel endothelial cells (HBMEC). The reactive oxygen species (ROS) production, nitric oxide (NO) generation, senescence, apoptosis and functions of HBMEC were analyzed. For mechanism study, the epidermal growth factor receptor (EGFR)/ extracellular regulated protein kinases (ERK)/ p38 mitogen-activated protein kinase (p38 MAPK), and phosphatidylinositol-3-kinase (PI3K), serine/threonine kinase (Akt), endothelial nitric oxide synthase (eNOS), and p-Akt were analyzed...
November 30, 2016: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/27903585/autophagy-in-chronic-liver-diseases-the-two-faces-of-janus
#3
Philippe Gual, Helene Gilgenkrantz, Sophie Lotersztajn
Alcoholic liver disease (ALD) and non-alcoholic fatty liver disease (NAFLD) are the leading causes of cirrhosis and increase the risk of hepatocellular carcinoma and liver-related death. ALD and NAFLD share common pathogenic features extending from isolated steatosis to steatohepatitis, steatofibrosis, which can progress to cirrhosis and hepatocellular carcinoma. The pathophysiological mechanisms of the progression of NAFLD and ALD are complex and still unclear. Important links between the regulation of autophagy (Macroautophagy and Chaperome-mediated autophagy) and chronic liver diseases have been reported...
November 30, 2016: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/27903584/src-independent-erk-signaling-through-the-%C3%AE-3-isoform-of-na-k-atpase
#4
Namrata Madan, Yunhui Xu, Qiming Duan, Moumita Banerjee, Isabel Larre, Sandrine V Pierre, Zijian Xie
The Na/K-ATPase α1 polypeptide supports both ion pumping and signaling functions. The Na/K-ATPase α3 polypeptide differs from α1 in both its primary structure and its tissue distribution. Alpha3 expression seems particularly important in neurons, and recent clinical evidence supports a unique role of this isoform in normal brain function. The nature of this specific role of α3 has remained elusive, because the ubiquitous presence of α1 has hindered efforts to characterize α3-specific functions in mammalian cell systems...
November 30, 2016: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/27903583/aspirin-therapy-reduces-the-ability-of-platelets-to-promote-colon-and-pancreatic-cancer-cell-proliferation-implications-for-the-oncoprotein-c-myc
#5
Annachiara Mitrugno, Joanna L Sylman, Anh T P Ngo, Jiaqing Pang, Rosalie C Sears, Craig Williams, Owen J T McCarty
Aspirin, an anti-inflammatory and anti-thrombotic drug, has become the focus of intense research as a potential anti-cancer agent owing to its ability to reduce tumor proliferation in vitro and to prevent tumorigenesis in patients. Studies have found an anti-cancer effect of aspirin when used in low, anti-platelet doses. However, the mechanism(s) through which low dose aspirin works is poorly understood. In this study we aimed to determine the effect of aspirin on the crosstalk between platelets and cancer cells...
November 30, 2016: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/27903582/threshold-levels-of-extracellular-l-arginine-that-trigger-nos-mediated-ros-rns-production-in-cardiac-ventricular-myocytes
#6
Jayalakshmi Ramachandran, R Daniel Peluffo
L-arginine (L-Arg) is the substrate for nitric oxide synthase (NOS) to produce nitric oxide (NO), a signaling molecule that is key in cardiovascular physiology and pathology. In cardiac myocytes, L-Arg is incorporated from the circulation through the functioning of system-y(+) cationic amino acid transporters. Depletion of L-Arg leads to NOS uncoupling, with O2 rather than L-Arg as terminal electron acceptor, resulting in superoxide formation. The reactive oxygen species (ROS) superoxide (O2•(-)), combined with NO, may lead to the production of the reactive nitrogen species (RNS) peroxynitrite (ONOO-), which is recognized as a major contributor to myocardial depression...
November 30, 2016: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/27903581/the-plasma-membrane-metal-ion-transporter-zip14-contributes-to-non-transferrin-bound-iron-uptake-by-human-%C3%AE-cells
#7
Richard Coffey, Mitchell D Knutson
The relationship between iron and β cell dysfunction has long been recognized as individuals with iron overload display an increased incidence of diabetes. This link is usually attributed to the accumulation of excess iron in β cells leading to cellular damage and impaired function. Yet, the molecular mechanism(s) by which human β cells take up iron has not been determined. In the present study, we assessed the contribution of the metal-ion transporters ZIP14, ZIP8, and DMT1 to iron uptake by human β cells...
November 30, 2016: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/27881413/the-drosophila-indirect-flight-muscle-myosin-heavy-chain-isoform-is-insufficient-to-transform-the-jump-muscle-into-a-highly-stretch-activated-muscle-type
#8
Cuiping Zhao, Douglas M Swank
Stretch activation (SA) is a delayed increase in force that enables high power and efficiency from a cyclically contracting muscle. SA exists in various degrees in almost all muscle types. In Drosophila, the indirect flight muscle (IFM) displays exceptionally high SA force production (FSA), whereas the jump muscle produces only minimal FSA We previously found that expressing an embryonic (EMB) myosin heavy chain (MHC) isoform in the jump muscle transforms it into a moderately SA muscle type and enables positive cyclical power generation...
November 23, 2016: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/27881412/renin-angiotensin-aldosterone-system-inhibitors-improve-membrane-stability-and-change-gene-expression-profiles-in-dystrophic-skeletal-muscles
#9
Jessica A Chadwick, Sayak Bhattacharya, Jeovanna Lowe, Noah Weisleder, Jill A Rafael-Fortney
Angiotensin-converting enzyme inhibitors and mineralocorticoid receptor (MR) antagonists are FDA approved drugs that inhibit the renin-angiotensin-aldosterone system (RAAS) and are used to treat heart failure. Combined treatment with the angiotensin-converting enzyme inhibitor lisinopril and the non-specific MR antagonist spironolactone surprisingly improves skeletal muscle, in addition to heart function and pathology in a Duchenne muscular dystrophy mouse model. We recently demonstrated that MR is present in all limb and respiratory muscles and functions as a steroid hormone receptor in differentiated normal human skeletal muscle fibers...
November 23, 2016: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/27881411/three-distinct-cell-populations-express-extracellular-matrix-proteins-and-increase-in-number-during-skeletal-muscle-fibrosis
#10
Mark A Chapman, Kavitha Mukund, Shankar Subramaniam, David Brenner, Richard L Lieber
Tissue extracellular matrix provides structural support and creates unique environments for resident cells. However, the identities of cells responsible for creating specific ECM components have not been determined. In striated muscle, the identity of these cells becomes important in disease when ECM changes result in fibrosis and subsequent increased tissue stiffness and dysfunction. Here we describe a novel approach to isolate and identify cells that maintain the ECM in both healthy and fibrotic muscle. Using a collagen I reporter mouse, we show that there are three distinct cell populations that express collagen I in both healthy and fibrotic skeletal muscle...
November 23, 2016: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/27881410/taz-activation-drives-fibroblast-spheroid-growth-expression-of-pro-fibrotic-paracrine-signals-and-context-dependent-ecm-gene-expression
#11
Amy J Jorgenson, Kyoung Moo Choi, Delphine Sicard, Karry M J Smith, Samantha E Hiemer, Xaralabos Varelas, Daniel J Tschumperlin
Recent studies have implicated the Hippo pathway and its transcriptional effectors YAP and TAZ as necessary for fibroblast activation and tissue fibrosis. To test the specific and sufficient roles for TAZ in driving autonomous fibroblast activation, we cultured NIH3T3 fibroblasts expressing a doxycycline-inducible nuclear-localized mutant of TAZ (TAZ4SA) in scaffold-free 3D hanging drop spheroids, or on matrices of specified mechanical rigidity. Control NIH3T3 fibroblasts formed spheroids in hanging drop culture that remained stable and neither increased nor decreased in size significantly over 15 days...
November 23, 2016: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/27856431/endoplasmic-reticulum-proteostasis-a-key-checkpoint-in-cancer
#12
Scott A Oakes
The unfolded protein response (UPR) is an intracellular signaling network largely controlled by three endoplasmic reticulum (ER) transmembrane proteins-IRE1, PERK, and ATF6-that monitors the protein folding status of the ER and initiates corrective measures to maintain ER homeostasis. Hypoxia, nutrient deprivation, proteasome dysfunction, sustained demands on the secretory pathway or somatic mutations in its client proteins--conditions often encountered by cancer cells-can lead to the accumulation of misfolded proteins in the ER and cause "ER stress...
November 16, 2016: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/27856430/glycolysis-and-oxidative-phosphorylation-are-essential-for-purinergic-receptor-mediated-angiogenic-responses-in-vasa-vasorum-endothelial-cells
#13
Martin Lapel, Philip Weston, Derek Strassheim, Vijaya Karoor, Nana Burns, Taras Lyubchenko, Petr Paucek, Kurt R Stenmark, Evgenia V Gerasimovskaya
Angiogenesis is an energy demanding process, however, the role of cellular energy pathways and their regulation by extracellular stimuli, especially extracellular nucleotides, remain largely unexplored. Using metabolic inhibitors of glycolysis (2-deoxyglucose, 2-DG) and oxidative phosphorylation (oligomycin, rotenone, and FCCP) we demonstrate that glycolysis and OXPHOS are both essential for angiogenic responses of vasa vasorum endothelial cell (VVEC). Treatment with P2R agonists, ATP and MeSADP, but not P1 receptor agonist, adenosine, increased glycolytic activity in VVEC (measured by extracellular acidification rate and lactate production)...
November 16, 2016: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/27856429/knock-down-of-ptrf-ameliorates-adipocyte-differentiation-and-functionality-of-human-mesenchymal-stem-cells
#14
Sergio Perez-Diaz, Beatriz Garcia-Rodriguez, Yolanda Gonzalez-Irazabal, Monica Valero, Javier Lagos, J M Arbones-Mainar
Healthy expansion of human adipose tissue requires mesenchymal stem cells (hMSC) able to proliferate and differentiate into mature adipocytes. Characterization of those factors that coordinate hMSC-to-adipocyte transition is hence of paramount importance to modulate the adipose tissue expansion. It has been previously reported that the adipogenic program of hMSC can be disrupted by up-regulating caveolar proteins and Polymerase I and Transcript Release Factor (PTRF) is an integral component of caveolae highly expressed in adipose tissue...
November 16, 2016: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/27834197/gaso-transmitters-expanding-the-kinetic-universe-of-cell-signaling
#15
Jeffrey S Isenberg, Josephine C Adams
not applicable to an Editorial.
November 9, 2016: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/27834196/adenosine-triphosphate-is-a-critical-determinant-for-vegfr-signal-during-hypoxia
#16
Abdullah Al Mamun, Hisaki Hayashi, Miho Sakima, Motohiko Sato
Hypoxia induces angiogenesis through the VEGF signaling pathway; however, signal propagation of VEGF under hypoxia is not understood fully. In this study, we examined alterations of VEGF signaling under hypoxia and its determinant in endothelial cells. To analyze VEGF signaling during hypoxia, human umbilical vein endothelial cells (HUVECs) were exposed to 3 h hypoxia (1% O2), followed by 3 h reoxygenation or 12 h hypoxia. Hypoxia induced the expression of VEGF mRNA; however, it was not associated with an increase of tube formation by HUVECs...
November 9, 2016: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/27834195/cftr-associated-ligand-cal-is-a-negative-regulator-of-mrp2-expression
#17
Man Li, Carol J Soroka, Kathy Harry, James L Boyer
The multidrug resistance-associated protein 2 (Mrp2) is an ATP-binding cassette transporter that transports a wide variety of organic anions across the apical membrane of epithelial cells. The expression of Mrp2 on the plasma membrane is regulated by protein-protein interactions. CFTR-associated ligand (CAL) interacts with transmembrane proteins via its PDZ domain and reduces their cell surface expression by increasing lysosomal degradation and intracellular retention. Our results showed that CAL is localized at the trans-Golgi network of rat hepatocytes...
November 9, 2016: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/27806940/nadph-oxidase-2-mediates-zinc-deficiency-induced-oxidative-stress-and-kidney-damage
#18
Mirandy S Li, Sherry E Adesina, Jennifer L Gooch, Robert S Hoover, Clintoria R Williams
Zn(2+) deficiency (ZnD) is comorbid with chronic kidney disease (CKD) and worsens kidney complications. Oxidative stress is implicated in the detrimental effects of ZnD. However, the sources of oxidative stress continue to be identified. Since NADPH oxidases (Nox) are the primary enzymes that contribute to renal reactive oxygen species (ROS) generation, this study's objective was to determine the role of these enzymes in ZnD-induced oxidative stress. We hypothesized that ZnD promotes Nox upregulation resulting in oxidative stress and kidney damage...
November 2, 2016: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/27806939/functional-loss-of-dhrs7c-induces-intracellular-ca2-overload-and-myotube-enlargement-in-c2c12-cells-via-calpain-activation
#19
Shinobu Arai, Masataka Ikeda, Tomomi Ide, Yuka Matsuo, Takeo Fujino, Katsuya Hirano, Kenji Sunagawa, Hiroyuki Tsutsui
Dehydrogenase/reductase member 7C (DHRS7C) is a newly identified NAD/NADH-dependent dehydrogenase that is expressed in cardiac and skeletal muscle and localized in the endoplasmic/sarcoplasmic reticulum (ER/SR). However, its functional role in muscle cells remains to be fully elucidated. Here, we investigated the role of DHRS7C by analyzing mouse C2C12 myoblasts deficient in DHRS7C (DHRS7C-KO cells), overexpressing wild-type DHRS7C (DHRS7C-WT cells), or expressing mutant DHRS7C (DHRS7C-Y191F or DHRS7C-K195Q cells, harboring point mutations in the NAD/NADH-dependent dehydrogenase catalytic core domain [YXXXK])...
November 2, 2016: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/27784681/h-oh-h-oh-h-oh-a-holiday-perspective-focus-on-mouse-slc4a11-expressed-in-xenopus-oocytes
#20
Keith Nehrke
Okay, there's no abstract necessary for this.
October 26, 2016: American Journal of Physiology. Cell Physiology
journal
journal
34858
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"