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Cytotherapy

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https://www.readbyqxmd.com/read/28215928/human-platelet-lysate-is-a-successful-alternative-serum-supplement-for-propagation-of-monocyte-derived-dendritic-cells
#1
Urban Švajger
BACKGROUND AIMS: Clinical protocols for dendritic cell (DC) generation from monocytes require the use of animal serum-free supplements. Serum-free media can also require up to 1% of serum supplementation. In addition, recommendations based on the 3Rs (Refinement, Reduction, Replacement) principle also recommend the use of non-animal sera in in vitro studies. The aim of this study was to explore the potential use of platelet lysate (PL) for generation of optimally differentiated DCs from monocytes...
February 16, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28215654/dendritic-cell-vaccination-in-combination-with-docetaxel-for-patients-with-metastatic-castration-resistant-prostate-cancer-a-randomized-phase-ii-study
#2
Per Kongsted, Troels Holz Borch, Eva Ellebaek, Trine Zeeberg Iversen, Rikke Andersen, Özcan Met, Morten Hansen, Henriette Lindberg, Lisa Sengeløv, Inge Marie Svane
BACKGROUND AIMS: We investigated whether the addition of an autologous dendritic cell-based cancer vaccine (DCvac) induces an immune response in patients with metastatic castration-resistant prostate cancer treated with docetaxel. METHODS: Forty-three patients were randomized 1:1 to receive up to 10 cycles of docetaxel alone, 75 mg/m(2) every 3 weeks or in combination with DCvac. Monocytes were harvested following a leukapheresis procedure, matured ex vivo and subsequently transfected with messenger RNA encoding multiple tumor-associated antigens (TAAs)...
February 15, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28215653/dendritic-cells-infected-by-ad-sh-socs1-enhance-cytokine-induced-killer-cik-cell-immunotherapeutic-efficacy-in-cervical-cancer-models
#3
Yi Zheng, Bicheng Hu, Shenggao Xie, Xiaofan Chen, Yuqian Hu, Wanping Chen, Shanshan Li, Bo Hu
BACKGROUND AIMS: Cervical cancer constitutes a major problem in women's health worldwide, but the efficacy of the standard therapy is unsatisfactory. Cytokine-induced killer (CIK) cells exhibit antitumor activity against a variety of malignancies in preclinical models and have proven safe and effective in clinical trials. However, current CIK therapy has limitations and needs to be improved to meet the clinical requirements. The aim of this study was to investigate whether suppressing the expression of cytokine signaling 1 (SOCS1) in dendritic cells (DCs) can shorten in vitro CIK culture time and improve its antitumor efficacy...
February 15, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28214127/functionalized-superparamagnetic-iron-oxide-nanoparticles-provide-highly-efficient-iron-labeling-in-macrophages-for-magnetic-resonance-based-detection-in-vivo
#4
Jack Sharkey, Philip J Starkey Lewis, Michael Barrow, Salamah M Alwahsh, June Noble, Eilidh Livingstone, Ross J Lennen, Maurits A Jansen, Jaime Garcia Carrion, Neill Liptrott, Shareen Forbes, Dave J Adams, Amy E Chadwick, Stuart J Forbes, Patricia Murray, Matthew J Rosseinsky, Christopher E Goldring, B Kevin Park
BACKGROUND AIMS: Tracking cells during regenerative cytotherapy is crucial for monitoring their safety and efficacy. Macrophages are an emerging cell-based regenerative therapy for liver disease and can be readily labeled for medical imaging. A reliable, clinically applicable cell-tracking agent would be a powerful tool to study cell biodistribution. METHODS: Using a recently described chemical design, we set out to functionalize, optimize and characterize a new set of superparamagnetic iron oxide nanoparticles (SPIONs) to efficiently label macrophages for magnetic resonance imaging-based cell tracking in vivo...
February 15, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28209525/stromal-vascular-fraction-shows-robust-wound-healing-through-high-chemotactic-and-epithelialization-property
#5
Dong-Sic Chae, Seongho Han, Mina Son, Sung-Whan Kim
BACKGROUND: Although human stromal vascular fraction (SVF) has been regarded as an attractive stem cell source, its therapeutic mechanism in wound healing has not been fully elucidated. AIMS: In this study, we investigated the molecular characteristics and therapeutic property of SVF for wound healing. METHODS: Microarray data showed that SVF cells are enriched with a higher level of wound healing or epithelium development-related genes and micro RNA...
February 13, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28188072/adjuvant-combination-therapy-with-gemcitabine-and-autologous-%C3%AE-%C3%AE-t-cell-transfer-in-patients-with-curatively-resected-pancreatic-cancer
#6
Taku Aoki, Hirokazu Matsushita, Mayumi Hoshikawa, Kiyoshi Hasegawa, Norihiro Kokudo, Kazuhiro Kakimi
BACKGROUND AIMS: The outcome for pancreatic cancer after surgery remains highly unsatisfactory, and development of more effective therapies is urgently needed. Therefore, we conducted a phase I clinical study of a novel combination of gemcitabine (GEM) and autologous γδ T-cell therapy for patients with curatively resected pancreatic cancer (University Hospital Medical Information Clinical Trials Registry identifier 000000931). METHODS: From July 2008 to December 2012, 56 consenting patients were recruited...
February 7, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28188071/a-good-manufacturing-practice-grade-standard-protocol-for-exclusively-human-mesenchymal-stromal-cell-derived-extracellular-vesicles
#7
Karin Pachler, Thomas Lener, Doris Streif, Zsuzsanna A Dunai, Alexandre Desgeorges, Martina Feichtner, Michaela Öller, Katharina Schallmoser, Eva Rohde, Mario Gimona
BACKGROUND AIMS: Extracellular vesicles (EVs) released by mesenchymal stromal cells (MSCs) may contribute to biological processes such as tissue regeneration, immunomodulation and neuroprotection. Evaluation of their therapeutic potential and application in future clinical trials demands thorough characterization of EV content and production under defined medium conditions, devoid of xenogenic substances and serum-derived vesicles. Addressing the apparent need for such a growth medium, we have developed a medium formulation based on pooled human platelet lysate (pHPL), free from animal-derived xenogenic additives and depleted of EVs...
February 7, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28162915/simple-in-vitro-generation-of-human-leukocyte-antigen-g-expressing-t-regulatory-cells-through-pharmacological-hypomethylation-for-adoptive-cellular-immunotherapy-against-graft-versus-host-disease
#8
Panagiota Stamou, Dimitra Marioli, Alexandra L Patmanidi, Argyro Sgourou, Angeliki Vittoraki, Efthymia Theofani, Chryso Pierides, Stavros Taraviras, Paul A Costeas, Alexandros Spyridonidis
BACKGROUND: Major barriers in using classical FOXP3+ regulatory T cells (Tregs) in clinical practice are their low numbers in the circulation, the lack of specific cell surface markers for efficient purification and the loss of expression of Treg signature molecules and suppressive function after in vitro expansion or in a pro-inflammatory microenviroment. A surface molecule with potent immunosuppressive function is the human leukocyte antigen-G (HLA-G), which is normally expressed in placenta protecting the "semi-allogeneic" fetus from maternal immune attack...
February 2, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28139337/graft-versus-leukemia-effect-with-a-wt1-specific-t-cell-response-induced-by-azacitidine-and-donor-lymphocyte-infusions-after-allogeneic-hematopoietic-stem-cell-transplantation
#9
Tatsunori Ishikawa, Nobuharu Fujii, Masahide Imada, Michinori Aoe, Yusuke Meguri, Tomoko Inomata, Hiromi Nakashima, Keiko Fujii, Shohei Yoshida, Hisakazu Nishimori, Ken-Ichi Matsuoka, Eisei Kondo, Yoshinobu Maeda, Mitsune Tanimoto
BACKGROUND: Azacitidine (Aza) and donor lymphocyte infusion (DLI) therapy has recently been reported as an effective salvage therapy for relapsed acute myeloid leukemia (AML) and myelodysplastic syndrome (MDS). Despite the high response rate and relatively long period of remission, most patients relapse again. The immunologic mechanism of the response and limited efficacy remain unknown. CASE REPORT: Aza + DLI therapy was performed for a patient with therapy-related MDS (t-MDS), who had relapsed after allogeneic peripheral blood stem cell transplantation...
January 27, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28131632/cd56-enriched-donor-cell-infusion-after-post-transplantation-cyclophosphamide-for-haploidentical-transplantation-of-advanced-myeloid-malignancies-is-associated-with-prompt-reconstitution-of-mature-natural-killer-cells-and-regulatory-t-cells-with-reduced-incidence
#10
Sarita Rani Jaiswal, Shamsur Zaman, Murugaiyan Nedunchezhian, Aditi Chakrabarti, Prakash Bhakuni, Margoob Ahmed, Kanika Sharma, Sheh Rawat, Paul O'donnell, Suparno Chakrabarti
We conducted a pilot study on the feasibility of CD56-enriched donor cell infusion after post-transplantation cyclophosphamide (PTCy) for 10 patients with advanced myeloid malignancies undergoing haploidentical peripheral blood stem cell transplantation with cyclosporine alone as graft-versus-host disease (GVHD) prophylaxis and compared the outcome and immune reconstitution with a control group of 20 patients undergoing the same without CD56-enriched donor cell infusion. An early and rapid surge of mature NK cells as well as CD4(+) T cells and regulatory T cells (Tregs) was noted compared with the control group...
January 25, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28109751/cord-blood-natural-killer-cells-expressing-a-dominant-negative-tgf-%C3%AE-receptor-implications-for-adoptive-immunotherapy-for-glioblastoma
#11
Eric S Yvon, Rachel Burga, Allison Powell, Conrad R Cruz, Rohan Fernandes, Cecilia Barese, Tuongvan Nguyen, Mohamed S Abdel-Baki, Catherine M Bollard
Cord blood (CB) natural killer (NK) cells are promising effector cells for tumor immunotherapy but are currently limited by immune-suppressive cytokines in the tumor microenvironment, such as transforming growth factor (TGF-β). We observed that TGF-β inhibits expression of activating receptors such as NKG2D and DNAM1 and decreases killing activity against glioblastoma tumor cells through inhibition of perforin secretion. To overcome the detrimental effects of TGF-β, we engrafted a dominant negative TGF-β receptor II (DNRII) on CB-derived NK cells by retroviral transduction and evaluated their ability to kill glioblastoma cells in the presence of TGF-β...
January 19, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28109752/measuring-the-resting-naive-sub-population-of-t-regulatory-cells-improves-prediction-of-suppressive-function-of-clinical-grade-t-regulatory-products
#12
LETTER
Abigail A Lamikanra, Wei Zhang, Hoi Pat Tsang, Phillip Morgan, David Smith, Elinor Curnow, Jon Smythe, Andy Peniket, Robert Danby, David J Roberts
No abstract text is available yet for this article.
January 18, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28089755/biodistribution-and-retention-of-locally-administered-human-mesenchymal-stromal-cells-quantitative-polymerase-chain-reaction-based-detection-of-human-dna-in-murine-organs
#13
Michael Creane, Linda Howard, Timothy O'Brien, Cynthia M Coleman
BACKGROUND: Determining the distributive fate and retention of a cell therapy product after administration is an essential part of characterizing it's biosafety profile. Therefore, regulatory guidelines stipulate that biodistribution assays are a requirement prior to advancing a cell therapy to the clinic. Here the development of a highly sensitive quantitative polymerase chain reaction (qPCR)-based method of tracking the biodistribution and retention of human mesenchymal stromal cells (hMSCs) in mice, rats or rabbits is described...
January 12, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28089754/stem-cell-therapy-an-emerging-modality-in-glomerular-diseases
#14
REVIEW
Umang G Thakkar, Aruna V Vanikar, Hargovind L Trivedi
The kidney has been considered a highly terminally differentiated organ with low proliferative potential and thus unlikely to undergo regeneration. Glomerular disease progresses to end-stage renal disease (ESRD), which requires dialysis or renal transplantation for better quality of life for patients with ESRD. Because of the shortage of implantable kidneys and complications such as immune rejection, septicemia and toxicity of immunosuppression, kidney transplantation remains a challenge. Therapeutic options available for glomerular disease include symptomatic treatment and strategies to delay progression...
January 12, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28081982/umbilical-cord-derived-mesenchymal-stromal-cell-conditioned-medium-exerts-in-vitro-antiaging-effects-in-human-fibroblasts
#15
Meirong Li, Yali Zhao, Haojie Hao, Liang Dong, Jiejie Liu, Weidong Han, Xiaobing Fu
BACKGROUND AIMS: Chronic wounds are a common complication of diabetes. Fibroblast-myofibroblast differentiation is important for wound repair, which is commonly impaired in non-healing wounds, and the underlying mechanisms need to be further elucidated. METHODS: We used high glucose (HG) to simulated the diabetes microenvironment and explored its effects on the biological features of fibroblasts in vitro. RESULTS: The results showed that prolonged HG induced senescence in fibroblasts through activation of p21 and p16 in a reactive oxygen species (ROS)-dependent manner, further delayed the viability and migration in fibroblasts and also depressed fibroblast differentiation through the TGF-β/Smad signaling pathway...
January 9, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28089079/repeated-subarachnoid-administrations-of-autologous-mesenchymal-stromal-cells-supported-in-autologous-plasma-improve-quality-of-life-in-patients-suffering-incomplete-spinal-cord-injury
#16
Jesús Vaquero, Mercedes Zurita, Miguel A Rico, Celia Bonilla, Concepción Aguayo, Cecilia Fernández, Noemí Tapiador, Marta Sevilla, Carlos Morejón, Jesús Montilla, Francisco Martínez, Esperanza Marín, Salvador Bustamante, David Vázquez, Joaquín Carballido, Alicia Rodríguez, Paula Martínez, Coral García, Mercedes Ovejero, Marta V Fernández
BACKGROUND AIMS: Cell therapy with mesenchymal stromal cells (MSCs) offers new hope for patients suffering from spinal cord injury (SCI). METHODS: Ten patients with established incomplete SCI received four subarachnoid administrations of 30 × 10(6) autologous bone marrow MSCs, supported in autologous plasma, at months 1, 4, 7 and 10 of the study, and were followed until the month 12. Urodynamic, neurophysiological and neuroimaging studies were performed at months 6 and 12, and compared with basal studies...
January 6, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28040463/umbilical-cord-tissue-derived-mesenchymal-stromal-cells-maintain-immunomodulatory-and-angiogenic-potencies-after-cryopreservation-and-subsequent-thawing
#17
Rita N Bárcia, Jorge M Santos, Mariana Teixeira, Mariana Filipe, Ana Rita S Pereira, Augusto Ministro, Ana Água-Doce, Manuela Carvalheiro, Maria Manuela Gaspar, Joana P Miranda, Luis Graça, Sandra Simões, Susana Constantino Rosa Santos, Pedro Cruz, Helder Cruz
BACKGROUND AIMS: The effect of cryopreservation on mesenchymal stromal cell (MSC) therapeutic properties has become highly controversial. However, data thus far have indiscriminately involved the assessment of different types of MSCs with distinct production processes. This study assumed that MSC-based products are affected differently depending on the tissue source and manufacturing process and analyzed the effect of cryopreservation on a specific population of umbilical cord tissue-derived MSCs (UC-MSCs), UCX(®)...
December 28, 2016: Cytotherapy
https://www.readbyqxmd.com/read/28040462/comparison-between-pediatric-and-adult-adipose-mesenchymal-stromal-cells
#18
Olivier Abbo, Marion Taurand, Paul Monsarrat, Isabelle Raymond, Emmanuelle Arnaud, Sandra De Barros, Françoise Auriol, Philippe Galinier, Louis Casteilla, Valerie Planat-Benard
BACKGROUND: Adipose-derived mesenchymalstromal cells (ASC) are currently tested in regenerative medicine to promote tissue reconstruction after injury. Regardingautologous purpose, the possible loss of therapeutic function and cell properties during aging have been questioned in adults. To date no reliable information is available concerning ASC from pediatric patients and a better knowledge is required for clinical applications. METHODS: Subcutaneous adipose tissue was collected from 27 donors (0-1 years old) and 50 donors (1-12 years old) and compared with adult ASC for in vitro characteristics...
December 28, 2016: Cytotherapy
https://www.readbyqxmd.com/read/28034522/comparison-of-two-methodologies-for-the-enrichment-of-mononuclear-cells-from-thawed-cord-blood-products-the-automated-sepax-system-versus-the-manual-ficoll-method
#19
Indreshpal Kaur, Jane M Zulovich, Marissa Gonzalez, Kara M McGee, Nirmali Ponweera, Daljit Thandi, Enrique F Alvarez, Kathy Annandale, Frank Flagge, Katayoun Rezvani, Elizabeth Shpall
BACKGROUND AIMS: Umbilical cord blood (CB) is being used as a source of hematopoietic stem cells (HSCs) and immune cells to treat many disorders. Because these cells are present in low numbers in CB, investigators have developed strategies to expand HSCs and other immune cells such as natural killer (NK) cells. The initial step in this process is to enrich mononuclear cells (MNCs) while depleting unwanted cells. The manual method of MNC enrichment is routinely used by many centers; however, it is an open system, time-consuming and operator dependent...
December 26, 2016: Cytotherapy
https://www.readbyqxmd.com/read/28017598/biodegradable-poly-%C3%AE%C2%B5-caprolactone-microcarriers-for-efficient-production-of-human-mesenchymal-stromal-cells-and-secreted-cytokines-in-batch-and-fed-batch-bioreactors
#20
Alan Tin-Lun Lam, Jian Li, Jessica Pei-Wen Toh, Eileen Jia-Hui Sim, Allen Kuan-Liang Chen, Jerry Kok-Yen Chan, Mahesh Choolani, Shaul Reuveny, William R Birch, Steve Kah-Weng Oh
Large numbers of human mesenchymal stromal cells (MSCs) used for a variety of applications in tissue engineering and cell therapy can be generated by scalable expansion in a bioreactor using microcarriers (MCs) systems. However, the enzymatic digestion process needed to detach cells from the growth surface can affect cell viability and potentially the potency and differentiation efficiency. Thus, the main aim of our study was to develop biocompatible and biodegradable MCs that can support high MSC yields while maintaining their differentiation capability and potency...
December 22, 2016: Cytotherapy
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