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Cytotherapy

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https://www.readbyqxmd.com/read/27919572/non-fucosylated-cb-cd34-cells-represent-a-good-target-for-enforced-fucosylation-to-improve-engraftment-following-cord-blood-transplantation
#1
Simon N Robinson, Michael W Thomas, Paul J Simmons, Junjun Lu, Hong Yang, Jeannie A Javni, Elizabeth J Shpall, Patrick A Zweidler-Mckay
BACKGROUND AIMS: Despite ethnic diversity and ready availability of cryopreserved, human leukocyte antigen-typed cord blood (CB), delayed engraftment remains a significant hurdle to successful CB transplantation. Suboptimal homing of CB hematopoietic stem and progenitor cells (HSPCs) to the hematopoietic microenvironment (HM) is thought to be responsible and due to low levels of HSPC fucosylation. Fucosylation (decoration with sialyl-Lewis(X)) may improve HSPC homing to HM by increasing the strength of HSPC/E-selectin interactions, where E-selectin is constitutively expressed by HM microvasculature...
December 2, 2016: Cytotherapy
https://www.readbyqxmd.com/read/27914820/cell-therapy-in-critical-limb-ischemia-a-comprehensive-analysis-of-two-cell-therapy-products
#2
Claire Tournois, Bernard Pignon, Marie-Antoinette Sevestre, Rida Al-Rifai, Valerie Creuza, Gaël Poitevin, Caroline François, Philippe Nguyen
BACKGROUND: Cell therapy has been proposed as a salvage limb procedure in critical limb ischemia (CLI). In spite of the fact that clinical trials found some efficacy, the mechanism of action remains elusive. The objective of this study was to characterize two autologous cell therapy products (CTPs) obtained from patients with advanced peripheral arterial disease. METHODS: Bone marrow (BM-CTPs) (n = 20) and CTPs obtained by non-mobilized cytapheresis (peripheral blood [PB]-CTPs) (n = 20) were compared...
November 30, 2016: Cytotherapy
https://www.readbyqxmd.com/read/27894881/comparative-study-of-isolation-expansion-and-characterization-of-epithelial-cells
#3
Sanjay Gottipamula, Sumit K Saraswat, K N Sridhar
BACKGROUND AIMS: The human epithelial cells (EPCs) have been identified as the essential element for the regeneration of skin construct for burns, wounds and various tissue engineer-based products. METHODS: In this study, the isolation, expansion and characterization of EPCs from various sources such as juvenile foreskin (JSK), buccal mucosa (BM), penile skin (PS) and urothelium (UR) in serum-free and xeno-free EpiLife media were evaluated. RESULTS: The growth kinetics study revealed that EPCs from JSK and BM had notably higher growth rates compared with the others...
November 25, 2016: Cytotherapy
https://www.readbyqxmd.com/read/27887867/evaluation-of-function-and-recovery-of-adipose-derived-stem-cells-after-exposure-to-paclitaxel
#4
William M Harris, Ping Zhang, Michael Plastini, Telisha Ortiz, Nikolas Kappy, Jefferson Benites, Edward Alexeev, Shaohua Chang, Ross Brockunier, Jeffrey P Carpenter, Spencer A Brown
BACKGROUND AIMS: Adipose-derived stem cells (ASCs) are considered to play a positive role in wound healing as evidenced by their increasing use in breast reconstructive procedures. After chemotherapy for breast cancer, poor soft tissue wound healing is a major problem. In the present study, the functional capabilities and recovery of ASCs after exposure to chemotherapeutic agent paclitaxel (PTX) using in vitro and ex vivo models were demonstrated. METHODS: Human ASCs were isolated from periumbilical fat tissue and treated with PTX at various concentrations...
November 22, 2016: Cytotherapy
https://www.readbyqxmd.com/read/27887866/continuously-expanding-car-nk-92-cells-display-selective-cytotoxicity-against-b-cell-leukemia-and-lymphoma
#5
Sarah Oelsner, Miriam E Friede, Congcong Zhang, Juliane Wagner, Susanne Badura, Peter Bader, Evelyn Ullrich, Oliver G Ottmann, Hans Klingemann, Torsten Tonn, Winfried S Wels
BACKGROUND AIMS: Natural killer (NK) cells can rapidly respond to transformed and stressed cells and represent an important effector cell type for adoptive immunotherapy. In addition to donor-derived primary NK cells, continuously expanding cytotoxic cell lines such as NK-92 are being developed for clinical applications. METHODS: To enhance their therapeutic utility for the treatment of B-cell malignancies, we engineered NK-92 cells by lentiviral gene transfer to express chimeric antigen receptors (CARs) that target CD19 and contain human CD3ζ (CAR 63...
November 22, 2016: Cytotherapy
https://www.readbyqxmd.com/read/27887865/overcoming-the-bottleneck-of-platelet-lysate-supply-in-large-scale-clinical-expansion-of-adipose-derived-stem-cells-a-comparison-of-fresh-versus-three-types-of-platelet-lysates-from-outdated-buffy-coat-derived-platelet-concentrates
#6
Peter V Glovinski, Mikkel Herly, Anders B Mathiasen, Jesper D Svalgaard, Rehannah Borup, Maj-Lis M Talman, Jens J Elberg, Stig-Frederik T Kølle, Krzysztof T Drzewiecki, Anne Fischer-Nielsen
BACKGROUND: Platelet lysates (PL) represent a promising replacement for xenogenic growth supplement for adipose-derived stem cell (ASC) expansions. However, fresh platelets from human blood donors are not clinically feasible for large-scale cell expansion based on their limited supply. Therefore, we tested PLs prepared via three methods from outdated buffy coat-derived platelet concentrates (PCs) to establish an efficient and feasible expansion of ASCs for clinical use. METHODS: PLs were prepared by the freeze-thaw method from freshly drawn platelets or from outdated buffy coat-derived PCs stored in the platelet additive solution, InterSol...
November 22, 2016: Cytotherapy
https://www.readbyqxmd.com/read/27887864/optimization-of-cgmp-purification-and-expansion-of-umbilical-cord-blood-derived-t-regulatory-cells-in-support-of-first-in-human-clinical-trials
#7
David H McKenna, Darin Sumstad, Diane M Kadidlo, Bjorn Batdorf, Colin J Lord, Sarah C Merkel, Christine M Koellner, Julie M Curtsinger, Carl H June, James L Riley, Bruce L Levine, Jeffrey S Miller, Claudio G Brunstein, John E Wagner, Bruce R Blazar, Keli L Hippen
BACKGROUND AIMS: Thymic-derived regulatory T cells (tTreg) are critical regulators of the immune system. Adoptive tTreg transfer is a curative therapy for murine models of autoimmunity, graft rejection, and graft-versus-host disease (GVHD). We previously completed a "first-in-human" clinical trial using in vitro expanded umbilical cord blood (UCB)-derived tTreg to prevent GVHD in patients undergoing UCB hematopoietic stem cell transplantation (HSCT). tTreg were safe and demonstrated clinical efficacy, but low yield prevented further dose escalation...
November 22, 2016: Cytotherapy
https://www.readbyqxmd.com/read/27884702/assessing-the-purity-of-regulatory-t-cells-a-humble-reminder
#8
LETTER
Erik Berglund, Felix Sellberg, David Berglund
No abstract text is available yet for this article.
November 21, 2016: Cytotherapy
https://www.readbyqxmd.com/read/27864016/generation-of-antigen-specific-cytotoxic-t-lymphocytes-with-activated-b-cells
#9
Sun Ok Yun, Hee Young Shin, Chang-Yuil Kang, Hyoung Jin Kang
BACKGROUND AIMS: Dendritic cells are well known as the most potent antigen-presenting cells. Nonetheless, their use in immunotherapy has been limited by the time-consuming and laborious steps involved in their generation in vitro. Therefore, much attention has been placed on alternative antigen-presenting cells that are relatively more convenient to manipulate. METHODS: In this study, the efficacy of B cells as antigen-presenting cells, compared with dendritic cells, in the induction of cytotoxic T lymphocytes against cytomegalovirus-specific antigens was evaluated...
November 15, 2016: Cytotherapy
https://www.readbyqxmd.com/read/27856230/a-comparison-in-therapeutic-efficacy-of-several-time-points-of-intravenous-stembell-administration-in-a-rat-model-of-acute-myocardial-infarction
#10
Reindert W Emmens, Maikel Oedayrajsingh-Varma, Linde Woudstra, Otto Kamp, Elisa Meinster, Annemieke van Dijk, Marco N Helder, Diana Wouters, Sacha Zeerleder, S Marieke van Ham, Nico de Jong, Hans W M Niessen, Lynda J M Juffermans, Paul A J Krijnen
BACKGROUND: Adipose-derived stromal cells (ASCs) are a promising new therapeutic option for patients with acute myocardial infarction (AMI). Previously, we found that ASCs coupled to antibody-targeted microbubbles (StemBells [StBs]) improved cardiac function when administered intravenously 7 days post-AMI in rats. In this study, we compared the efficacy of intravenous StB administration at different administration time points following AMI in rats. METHODS: AMI, followed by reperfusion, was induced in four groups of male Wistar rats, which subsequently received an intravenous 1 × 10(6) StB bolus 1 day post-AMI (StB1; n = 8), 7 days post-AMI (StB7; n = 9), at both time points (StB1+7; n = 7) or neither (Control; n = 7)...
November 14, 2016: Cytotherapy
https://www.readbyqxmd.com/read/27856229/absolute-density-of-different-sources-of-hematopoietic-progenitor-cells-bone-marrow-peripheral-blood-stem-cell-and-umbilical-cord-blood
#11
LETTER
Anelise Bergmann Araújo, Melissa Helena Angeli, Gabrielle Dias Salton, Juliana Monteiro Furlan, Tissiana Schmalfuss, Liane Marise Röhsig
No abstract text is available yet for this article.
November 14, 2016: Cytotherapy
https://www.readbyqxmd.com/read/27856228/mesenchymal-stromal-cell-secretomes-are-modulated-by-suspension-time-delivery-vehicle-passage-through-catheter-and-exposure-to-adjuvants
#12
Kaushik Parsha, Osman Mir, Nikunj Satani, Bing Yang, Waldo Guerrero, Zhuyong Mei, Chunyan Cai, Peng R Chen, Adrian Gee, Patrick J Hanley, Jaroslaw Aronowski, Sean I Savitz
BACKGROUND AIMS: Extensive animal data indicate that mesenchymal stromal cells (MSCs) improve outcome in stroke models. Intra-arterial (IA) injection is a promising route of delivery for MSCs. Therapeutic effect of MSCs in stroke is likely based on the broad repertoire of secreted trophic and immunomodulatory cytokines produced by MSCs. We determined the differential effects of exposing MSCs to different types of clinically relevant vehicles, and/or different additives and passage through a catheter relevant to IA injections...
November 14, 2016: Cytotherapy
https://www.readbyqxmd.com/read/27842980/establishment-characterization-and-long-term-culture-of-human-endocrine-pancreas-derived-microvascular-endothelial-cells
#13
Valeria Sordi, Anna Ferri, Valentina Ceserani, Emilio Ciusani, Erica Dugnani, Silvia Pellegrini, Rita Nano, Lorenza Pecciarini, Augusto Pessina, Luisa Pascucci, Lorenzo Piemonti, Giulio Alessandri
BACKGROUND: In vitro primary cultures of microvascular endothelial cells from endocrine pancreas are difficult to obtain, but can be a very helpful tool for studies of islet biology, transplantation and regenerative medicine. METHODS: We applied a protocol recently described for the isolation and culture of brain microvascular endothelial cells (EC) on human pancreatic islets. EC obtained were characterized in terms of morphological (light and transmission electron microscopy), phenotypical (by immunofluorescence and flow cytometry) and functional (cord formation assay and protein secretion by multiplex bead-based assay) characteristics...
November 11, 2016: Cytotherapy
https://www.readbyqxmd.com/read/27840135/erratum-to-tumor-antigen-specific-t-cells-for-immune-monitoring-of-dendritic-cell-treated-glioblastoma-patients
#14
I Müller, D Altherr, M Eyrich, B Flesch, K S Friedmann, R Ketter, J Oertel, E C Schwarz, A Technau, S Urbschat, H Eichler
No abstract text is available yet for this article.
November 10, 2016: Cytotherapy
https://www.readbyqxmd.com/read/27840134/comparison-of-the-anti-inflammatory-effects-of-induced-pluripotent-stem-cell-derived-and-bone-marrow-derived-mesenchymal-stromal-cells-in-a-murine-model-of-corneal-injury
#15
Young In Yun, Se Yeon Park, Hyun Ju Lee, Jung Hwa Ko, Mee Kum Kim, Won Ryang Wee, Roxanne L Reger, Carl A Gregory, Hosoon Choi, Samuel F Fulcher, Darwin J Prockop, Joo Youn Oh
BACKGROUND AIMS: Mesenchymal stromal cells (MSCs) offer tremendous potential for therapeutic applications for inflammatory diseases. However, tissue-derived MSCs, such as bone marrow-derived MSCs (BM-MSCs), have considerable donor variations and limited expandability. It was recently demonstrated that MSCs derived from induced pluripotent stem cells (iPSC-MSCs) have less pro-tumor potential and greater expandability of homogenous cell population. In this study, we investigated the anti-inflammatory effects and mechanism of iPSC-MSCs in a murine model of chemical and mechanical injury to the cornea and compared the effects with those of BM-MSCs...
November 10, 2016: Cytotherapy
https://www.readbyqxmd.com/read/27836573/human-mesenchymal-stromal-cells-undergo-apoptosis-and-fragmentation-after-intravenous-application-in-immune-competent-mice
#16
Johannes Leibacher, Katrin Dauber, Sabrina Ehser, Veronika Brixner, Katarina Kollar, Anja Vogel, Gabi Spohn, Richard Schäfer, Erhard Seifried, Reinhard Henschler
BACKGROUND AIMS: The biodistribution of human MSCs after systemic delivery is incompletely understood. We investigated the changes in cell size and cell surface markers of human MSCs after intravenous (IV) injection in immune competent mice. METHODS: Male human MSCs were labeled with fluorescent vital dye PKH67 and tracked after IV administration in C57/BL6 mice. MSCs were tracked in blood and different murine tissues by human SRY gene quantitative polymerase chain reaction (qPCR) analysis, flow cytometry and fluorescence microscopy...
November 8, 2016: Cytotherapy
https://www.readbyqxmd.com/read/27816409/progressive-increase-in-brain-glucose-metabolism-after-intrathecal-administration-of-autologous-mesenchymal-stromal-cells-in-patients-with-diffuse-axonal-injury
#17
Jesús Vaquero, Mercedes Zurita, Celia Bonilla, Cecilia Fernández, Juan J Rubio, Jorge Mucientes, Begoña Rodriguez, Edelio Blanco, Luis Donis
BACKGROUND AIMS: Cell therapy in neurological disability after traumatic brain injury (TBI) is in its initial clinical stage. We describe our preliminary clinical experience with three patients with diffuse axonal injury (DAI) who were treated with intrathecal administration of autologous mesenchymal stromal cells (MSCs). METHODS: Three patients with established neurological sequelae due to DAI received intrathecally autologous MSCs. The total number of MSCs administered was 60 × 10(6) (one patient), 100 × 10(6) (one patient) and 300 × 10(6) (one patient)...
November 2, 2016: Cytotherapy
https://www.readbyqxmd.com/read/27793552/herpes-simplex-virus-type-1-hsv-1-specific-t-cell-generation-from-hla-a1-and-hla-a2-positive-donors-for-adoptive-immunotherapy
#18
Chun K K Ma, Leighton Clancy, Shivashni Deo, Emily Blyth, Kenneth P Micklethwaite, David J Gottlieb
BACKGROUND AIMS: Herpes simplex virus (HSV) reactivation and infection is common in patients undergoing hematopoietic stem cell transplant (HSCT) and requires routine antiviral prophylaxis. Drug-resistant strains are increasingly common, and effective alternative therapy is currently unavailable. We generated and characterized HSV-1-specific T cells for use in adoptive cellular immunotherapy following allogeneic stem cell transplantation. METHODS: Peripheral blood mononuclear cells from HLA-A1 and HLA-A2 HSV-seropositive hereditary hemochromatosis donors were used as the antigen source...
October 25, 2016: Cytotherapy
https://www.readbyqxmd.com/read/27771213/senescence-and-quiescence-in-adipose-derived-stromal-cells-effects-of-human-platelet-lysate-fetal-bovine-serum-and-hypoxia
#19
Rebekka Harary Søndergaard, Bjarke Follin, Lisbeth Drozd Lund, Morten Juhl, Annette Ekblond, Jens Kastrup, Mandana Haack-Sørensen
BACKGROUND AIMS: Adipose-derived stromal cells (ASCs) are attractive sources for cell-based therapies. The hypoxic niche of ASCs in vivo implies that cells will benefit from hypoxia during in vitro expansion. Human platelet lysate (hPL) enhances ASC proliferation rates, compared with fetal bovine serum (FBS) at normoxia. However, the low proliferation rates of FBS-expanded ASCs could be signs of senescence or quiescence. We aimed to determine the effects of hypoxia and hPL on the expansion of ASCs and whether FBS-expanded ASCs are senescent or quiescent...
October 19, 2016: Cytotherapy
https://www.readbyqxmd.com/read/27769638/importance-of-homogeneity-between-groups-in-the-study-of-therapeutic-efficacy
#20
Ding Pan, Ju-Yu Tang
No abstract text is available yet for this article.
October 18, 2016: Cytotherapy
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