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Japanese Journal of Infectious Diseases

Tian-Cheng Li, Sayaka Yoshizaki, Yasushi Ami, Yuriko Suzaki, Reimar Johne, Takaji Wakita
To investigate whether rat hepatitis E virus (rat HEV) is excreted into the urine of rats, we infected three Wistar and six nude rats with rat HEV and examined the rat HEV RNA in serum, fecal and urine samples. We detected rat HEV RNA in the serum and fecal samples of all rats but not in the urine. Our results suggest that in rats, rat HEV is not transmitted via urine.
August 31, 2016: Japanese Journal of Infectious Diseases
Takeshi Hatta, Kenkichi Imamura, Takehisa Yamamoto, Makoto Matsubayashi, Naotoshi Tsuji, Toshiyuki Tsutsui
This study presents the results of a large-scale one-year survey of Trichinella spp. in Japanese wild boar (Sus scrofa). We analyzed the tongues of 1168 wild boars captured by hunters in 30 prefectures of Japan, most of which were boar habitats, from October 2014 to January 2015. The samples were digested, and the Trichinella spp. muscle larvae prevalences were examined. Pooled samples from 10 individuals (15 g per head) and 117 randomly selected samples (10% of the total samples) that were processed individually showed no larval infection...
August 31, 2016: Japanese Journal of Infectious Diseases
Suvaporn Anugulruengkitt, Thanyawee Puthanakit, Sompis Siengboon, Leilani Paitoonpong, Ekasit Kowitdamrong, Chitsanu Pancharoen, Yuwaree Pichitchok
This study aimed to determine the proportion of varicella non-immune pediatric healthcare workers (HCWs) of Pediatrics Department, King Chulalongkorn Memorial Hospital and to determine cost effective strategies to identify non-immune personnel. A cross-sectional study, using a self-administered questionnaire to determine history of chickenpox or 2-dose varicella vaccination was conducted. From a total of 699 HCWs, 653 HCWs (93%) including 145 physicians (22%), 297 nurses (46%) and 211 administrative staff (32%) responded to questionnaires...
August 31, 2016: Japanese Journal of Infectious Diseases
Xiaoxia Huang, Zhongxin Zhang, Guanghui Jin, Xiaofang Wang, Chusheng Tan, Hongmei Yin, Shiwen Wang
To determine SFTSV seroprevalence and identify potential risk factors in non-endemic areas, a cross-sectional study was conducted in healthy population in a mountainous area without any SFTS cases reported in Anhui province. We recruited and collected blood samples from 270 participants in four age groups. An enzyme-linked immunosorbent assay kit was used to detect IgG antibody against SFTSV. The total seropositive rate of SFTSV IgG antibody was 6.3% (17/270). Significant difference was found between tea pluckers (9...
August 31, 2016: Japanese Journal of Infectious Diseases
Chanwit Tribuddharat, Pintip Pongpech, Angkana Charoenwatanachokchai, Somchai Lokpichart, Somporn Srifuengfung, Suthatta Sonprasert
We studied antimicrobial susceptibility and the current rates of blaTEM-1 and blaTEM-135. Neisseria gonorrhoeae 370 isolates were tested by using disk diffusion method and found 100% susceptible to cefixime, ceftriaxone, cefotaxime, ceftazidime, cefepime, spectinomycin and azithromycin. They were resistant to penicillin (85.7%), ciprofloxacin (88.0%), ofloxacin (97.4%), and tetracycline (89.1%). Penicillinase-producing N. gonorrhoeae (PPNG) was 83.8% which 70.0% was further identified as PPNG plus tetracycline resistant N...
August 31, 2016: Japanese Journal of Infectious Diseases
Atsushi Hinenoya, Noritomo Yasuda, Takumi Hibino, Ayaka Shima, Akira Nagita, Teizo Tsukamoto, Shinji Yamasaki
Here, we show that a bacterium isolated as a sole pathogen from a child with diarrhea harbor eae and 2 different cytolethal distending toxin genes (cdt) homologous to Escherichia coli cdt-I and cdt-II genes, respectively. The bacterium was originally identified as atypical E. coli by conventional biochemical tests. However, this bacterium was finally identified as E. albertii by multilocus sequence analysis, which is an only method being able to clearly differentiate E. coli from E. albertii. Shiga toxin 2f (stx2f) genes were also detected in the strain...
August 31, 2016: Japanese Journal of Infectious Diseases
Satomi Saito, Yoshio Koori, Yusuke Ohsaki, Shunsuke Osaka, Kozue Oana, Yukiko Nagano, Yoshichika Arakawa, Noriyuki Nagano
Detection and characterization of β-lactamase genes in a total of ten non-typhoidal Salmonella (NTS) clinical isolates exhibiting resistance to the third-generation cephalosporins collected during 2012 -2014 in Japan were conducted. Among five strains with cefotaxime MIC (minimum inhibitory concentration) ≥64 μg/ml and positive results of clavulanic acid inhibition effect, blaCTX-M-2 was detected in three strains (serotypes Stanley and Muenchen), and each of blaTEM-52 (serotype Manhattan) and blaSHV-12 (serotype Infantis) was found in one strain...
August 31, 2016: Japanese Journal of Infectious Diseases
Ichiro Furukawa, Tomoe Ishihara, Hiroshi Teranishi, Shioko Saito, Jun Yatsuyanagi, Eriko Wada, Yuko Kumagai, Shiho Takahashi, Takayuki Konno, Hiroko Kashio, Akihiko Kobayashi, Naoki Kato, Ken-Ichi Hayashi, Keisuke Fukushima, Kazuhiko Ishikawa, Kazumi Horikawa, Akira Oishi, Hidemasa Izumiya, Takahiro Ohnishi, Yoshiko Konishi, Toshiro Kuroki
This study was undertaken to determine the prevalence, antimicrobial susceptibility, and genetic relatedness of Salmonella enterica subsp. enterica and Campylobacter spp. in poultry meat, and to analyze the association of genetic types of these bacteria with their geographical distribution and antimicrobial resistance profiles. Fifty-four and 71 of 100 samples were found to be contaminated with Salmonella and Campylobacter, respectively. Nine Salmonella serotypes were found, including S. enterica subsp. enterica serovar Infantis (33%), Schwarzengrund (12%), Manhattan (9%), and others...
August 31, 2016: Japanese Journal of Infectious Diseases
Nitin Gupta, Rama Chaudhry, Sushil K Kabra, Rakesh Lodha, Bijay R Mirdha, Bimal K Das, A B Dey, V Sreenivas
The major brunt of Scrub typhus is borne by the developing countries like India but the disease still remains neglected. The rapid progression to fatal complications and associated mortality calls for improved diagnostic services. IgM Immunofluorescence assay (IFA) with all its limitations is still the gold standard. Out of 218 suspected cases enrolled in the 2 year study period, 30 cases of scrub typhus were detected by IgM IFA at 1:64 dilution. The sensitivity of IgM Enzyme linked immunosorbant assay (ELISA), IgM Rapid Flow assay (RFA) and IgG RFA was found to be 97%, 87% and 77% respectively...
August 31, 2016: Japanese Journal of Infectious Diseases
Fatemeh Torkaman Asadi, Seyyed Hamid Hashemi, Mohammad Yousef Alikhani, Abbas Moghimbeigi, Zahra Naseri
Current drug regimens for brucellosis are associated with relatively high rates of therapeutic failure or relapse. Reduced antimicrobial susceptibility of Brucella spp. has been proposed recently as a potential cause of therapeutic failure. The aim of this study was to evaluate the antibiotic resistance pattern of Brucella melitensis clinical isolates by E-test method in Hamadan, west of Iran. In a 15-month period, all patients with suspected brucellosis were enrolled. Blood Specimens were collected for diagnosis of brucellosis by BACTEC system and serological tests...
August 31, 2016: Japanese Journal of Infectious Diseases
Sivaporn Inyoo, Aroonroong Suttitheptumrong, Sa-Nga Pattanakitsakul
Dengue hemorrhagic fever/dengue shock syndrome (DHF/DSS) is a severe pathological manifestation of dengue virus (DENV) infection. Enhanced production of cytokines in dengue patients is proposed to induce endothelial barrier instability resulting in increased vascular leakage. Tumor necrosis factor (TNF)-α is an inflammatory cytokine that activates endothelial cells and enhances vascular permeability and plasma leakage in DHF/DSS. The present study investigated the in vitro effect of TNF-α and DENV infection on the expression of adherence junction proteins, tight junction proteins and membrane integrity of human endothelial cell lines...
August 31, 2016: Japanese Journal of Infectious Diseases
Yumi Akiyama, Hiroko Futai, Etsuko Saito, Kenichi Ogita, Hiroshi Sakae, Masaharu Fukunaga, Hidetaka Tsuji, Masatsugu Chikahira, Atsushi Iguchi
Subtypes of stx1 and stx2 carried in 45 Shiga toxin-producing Escherichia coli (STEC) strains isolated from cattle were investigated using PCR. Only subtype stx1a was detected among all the stx1-positive strains. The major stx2 subtype was stx2a, and followed by stx2d, stx2c, stx2b and stx2g in decreasing order. stx2c was found in the strains of O157 and O174. stx2d was found in 11 strains; they were confirmed using DNA sequencing to carry both the activatable tail and the END motif, all of which were eae-negative and three contained stx2d as the only stx...
August 31, 2016: Japanese Journal of Infectious Diseases
Xiaobing Guo, Zaiqiu Cao, Zhifeng Dai, Yuan Li, Xiaohong He, Xiaoxin Hu, Fuyun Tian, Yihui Ren
Extended-spectrum-beta-lactamase-producing and carbapenemase-producing Klebsiella pneumoniae has rapidly spread through clinical units worldwide. This study investigated the epidemiology and resistance profiles of K. pneumoniae strains isolated in Central China between 2009 and 2014. Antimicrobial susceptibility tests and polymerase chain reaction were used to investigate the prevalence and resistance of ESBLs and carbapenemases produced by K. pneumoniae, and the prevalence of KPC-producing K. pneumoniae was investigated by multilocus sequence typing...
August 31, 2016: Japanese Journal of Infectious Diseases
Mitsuo Uchida, Minoru Kaneko, Yoshihiko Hidaka, Hiroshi Yamamoto, Takayuki Honda, Shouhei Takeuchi, Masaya Saito, Shigeyuki Kawa
Seasonal influenza is known to spread within and among educational organizations. Detailed understanding of the pattern of infection requires prospective epidemiological studies among all schools in a community. This prospective survey evaluated 13,217 schoolchildren attending all 29 public elementary schools in Matsumoto City, Japan, in 2014/2015. Questionnaires were distributed to school nurses to obtain information about onset date and suspected route of transmission of influenza of all schoolchildren diagnosed at medical institutions...
August 31, 2016: Japanese Journal of Infectious Diseases
Yoshifumi Uwamino, Kayoko Sugita, Naoki Hasegawa, Tomoyasu Nishimura, Hiroshi Fujiwara, Satoshi Iwata
No abstract text is available yet for this article.
August 31, 2016: Japanese Journal of Infectious Diseases
Jung-Hyun Byun, Hyunwoong Park, Sunjoo Kim
Although Shewanella algae has been known for weak pathogenicity, case reports have been steadily increased. Shewanella algae and Shewanella haliotis are not easy to distinguish by conventional phenotypical methods. We reviewed microbiological and clinical features of S. algae and S. haliotis infections in our institute. Bacterial culture and identification reports from patient samples from 2010 to 2014 were reviewed to screen the cases of Shewanella infections. In addition to conventional biochemical tests, 16S rRNA gene sequence analysis and MALDI-TOF were performed with 19 bacterial isolates stored...
August 31, 2016: Japanese Journal of Infectious Diseases
Chihiro Katsukawa, Sadasaburo Asai, Kayoko Mizutani, Kazuko Arai, Urara Kohdera, Chieko Kushibiki, Masashi Shiomi, Yoshihiro Takeda, Atsuko Naka, Keiji Nakano, Tohru Matsushita, Kazuo Takahashi
No abstract text is available yet for this article.
September 21, 2016: Japanese Journal of Infectious Diseases
Yu Suzuki, Miwa Shimanuki, Junji Seto, Kazue Yahagi, Katsumi Mizuta
No abstract text is available yet for this article.
September 21, 2016: Japanese Journal of Infectious Diseases
Yohei Matoba, Yoko Aoki, Shizuka Tanaka, Kazue Yahagi, Yuriko Katsushima, Fumio Katsushima, Kanetsu Sugawara, Yoko Matsuzaki, Katsumi Mizuta
No abstract text is available yet for this article.
September 21, 2016: Japanese Journal of Infectious Diseases
Yasuyuki Morishima, Yuko Tomaru, Shin-Ichiro Fukumoto, Hiromu Sugiyama, Hiroshi Yamasaki, Chihiro Hashimoto, Kasumi Harada
No abstract text is available yet for this article.
September 21, 2016: Japanese Journal of Infectious Diseases
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