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Japanese Journal of Infectious Diseases

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https://www.readbyqxmd.com/read/28003605/contribution-of-streptococcus-gordonii-hsa-adhesin-to-biofilm-formation
#1
Riyo Oguchi, Yukihiro Takahashi, Kisaki Shimazu, Yumiko Urano-Tashiro, Taketo Kawarai, Kiyoshi Konishi, Hiroyuki Karibe
Adhesion of oral mitis group streptococci such as Streptococcus gordonii to acquired pellicle of the tooth surface is the initial step of oral biofilm formation. S. gordonii wild type strain DL1 possesses Hsa adhesin, recognizing terminal sialic acid of the host sialoglycoconjugates. The aim of the present study was to investigate the role of Hsa adhesin in biofilm formation. Biofilm-forming ability of the S. gordonii hsa mutant in microtiter plates pre-coated with saliva, fetuin and mucin was significantly reduced, compared to that of DL1...
December 22, 2016: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28003604/genetic-characterization-of-rare-genotype-gii-5-sapovirus-strain-detected-from-food-borne-suspected-gastroenteritis-outbreak-among-adults-in-japan-2010
#2
Tomoichiro Oka, Yen Hai Doan, Kei Haga, Kohji Mori, Tomoko Ogawa, Akimi Yamazaki
No abstract text is available yet for this article.
December 22, 2016: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28003603/a-single-vaccination-of-nonhuman-primates-with-highly-attenuated-smallpox-vaccine-lc16m8-provides-long-term-protection-against-monkeypox
#3
Itoe Iizuka, Yasushi Ami, Yuriko Suzaki, Noriyo Nagata, Shuetsu Fukushi, Momoko Ogata, Shigeru Morikawa, Hideki Hasegawa, Masashi Mizuguchi, Ichiro Kurane, Masayuki Saijo
Monkeypox virus (MPXV) causes human monkeypox (human MPX), which is a similar disease with smallpox in humans. A previous study showed that a single vaccination of monkeys with LC16m8, a highly attenuated smallpox vaccine, protected them from MPX at 4-5 weeks after vaccination. In the present study, we evaluated the long-term efficacy of a single vaccination with LC16m8 in a nonhuman primate model of MPXV infection. The monkeys were inoculated with LC16m8, Lister (parental strain of LC16m8), or mock and then challenged with MPXV via the subcutaneous route at 6 and 12 months after vaccination, which we compared with either Lister or mock vaccination...
December 22, 2016: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28003602/evaluation-of-immunochromatography-tests-and-new-enzyme-immunoassay-for-detection-of-novel-gii-17-norovirus-in-stool-samples
#4
Hiroshi Ushijima, Aksara Thongprachum, Pattara Khamrin, Sayaka Takanashi, Shoko Okitsu, Niwat Maneekarn, Satoshi Hayakawa
A novel GII.17 norovirus (NoV) Kawasaki 2014 has been spread in several regions worldwide. Rapid and reliable diagnostic tests are needed for the detection of this newly emerged NoV variant. In this study, the sensitivity of seven brands of immunochromatography (IC) test kits, 6 are on the market in Japan and 1 in Europe, were evaluated by testing with confirmed GII.17 NoV positive stool samples. The stool samples were also tested by bioluminescent enzyme immunoassay (BLEIA). Real-time RT-PCR was used as a gold standard method...
December 22, 2016: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28003601/molecular-epidemiological-study-of-mumps-epidemics-of-2015-in-okinawa-japan
#5
Yumani Kuba, Hisako Kyan, Eri Arakaki, Taketoshi Takara, Takashi Kato, Sho Okano, Yuko Oshiro, Jun Kudaka, Minoru Kidokoro
In Okinawa Prefecture in Japan, although major mumps epidemics have occurred every 4-5 years, no laboratory diagnoses have been conducted. The mumps epidemic spread in Okinawa from October 2014, and we collected clinical materials from 31 mumps patients in 4 areas (Hokubu, Nanbu, Miyako and Yaeyama) from July to December 2015 for virus isolation and RT-PCR, whose positive ratios were 52% and 87%, respectively. The phylogenetic analyses showed that all isolates were classified into genotype G, most of which, except for 1, consisted of two subgenotypes, Ge (55...
December 22, 2016: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28003600/identification-of-group-g-streptococci-isolates-from-companion-animals-in-japan-and-their-antimicrobial-resistance
#6
Yuzo Tsuyuki, Goro Kurita, Yoshiteru Murata, Mieko Goto, Takashi Takahashi
This study determined species-level identification and antimicrobial resistance (AMR) properties in group G streptococci (GGS) isolates from companion animals in Japan. Strains were isolated from sterile/non-sterile specimens of 72 animals with clinical symptoms or signs in April-May, 2015. We identified them based on 16S rRNA sequencing, mass spectrometry (MS), and an automated method based on their biochemical properties. Antimicrobial susceptibility was determined with broth microdilution method and Etest...
December 22, 2016: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28003599/establishment-of-subclones-of-severe-fever-with-thrombocytopenia-syndrome-virus-yg1-strain-selected-by-the-degree-of-low-ph-dependent-cell-fusion-activity
#7
Sanae Nishio, Yoshimi Tsuda, Ryo Ito, Kenta Shimizu, Kumiko Yoshimatsu, Jiro Arikawa
Severe fever with thrombocytopenia syndrome virus YG1 strain was isolated from the first clinical case in Japan. We found that only some of the cells underwent low-pH-dependent cell fusion, though all of the cells were confirmed to have been infected with viruses, suggesting that YG1 strain consists of heterogeneous mixtures of related viruses. Here, we established three subclones, E3, A4 and B7, from YG1 strain by the limiting dilution method with pH-dependent cell fusion activities. Subclone E3 showed weak fusion activity and cytopathic effect (CPE) in Vero E6 cells...
December 22, 2016: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28003598/an-itraq-based-quantitative-proteomics-study-of-refractory-mycoplasma-pneumoniae-pneumonia-patients
#8
Jia-Lu Yu, Qi-Fang Song, Zhi-Wei Xie, Wen-Hui Jiang, Jia-Hui Chen, Hui-Feng Fan, Ya-Ping Xie, Gen Lu
Mycoplasma pneumoniae(MP) is a leading cause of community-acquired pneumonia in children and young adults. Although MP pneumonia is usually benign and self-limited, in some cases it can develop into a life-threating refractory MP pneumonia(RMPP). However, the pathogenesis of RMPP is poorly understood. The identification and characterization of proteins related to RMPP could provide "proof of principle" to facilitate appropriate diagnostic and therapeutic strategies for MP patients. In this study, we applied the quantitative proteomic technique(iTRAQ) to globally analyze MP-related proteins in serum samples from five RMPP and five non-refractory MP pneumonia(NRMPP) patients, and five healthy children...
December 22, 2016: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28003597/safety-and-immunogenicity-study-of-a-9-valent-human-papillomavirus-vaccine-administered-to-9-to-15-year-old-japanese-girls
#9
Satoshi Iwata, Shinya Murata, Shi Rong Han, Akira Wakana, Miyuki Sawata, Yoshiyuki Tanaka
A 9-valent human papillomavirus (HPV 6/11/16/18/31/33/45/52/58) virus-like particle (VLP) vaccine (9vHPV) has been proven highly efficacious in preventing anogenital disease related with vaccine HPV types in a pivotal Phase III study in women aged 16 to 26 years. We report here the results of an open-label phase III study conducted to bridge the findings in women age 16 to 26 years to Japanese girls aged 9 to 15 years. All subjects (n = 100) received a 3-dose regimen of 9vHPV vaccine at day 1, month 2 and month 6...
December 22, 2016: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28003596/a-case-of-rat-bite-fever-caused-by-streptobacillus-moniliformis
#10
Yasue Hayakawa, Jun Suzuki, Masahiro Suzuki, Wataru Sugiura, Kiyofumi Ohkusu
We report a case of rat bite fever (RBF) diagnosed by detection of Streptobacillus moniliformis from blood culture and synovial fluid. The patient was a 45-year-old man with alcoholic liver cirrhosis. He was bitten on his third finger by a rat, which was caught in a mousetrap installed in his house. After the bite, fever, rash and arthralgia developed over a period of about two weeks. The patient was admitted to our hospital and treated with combination of Ampicillin- Sulbactam (SAM), Vancomycin (VAN) and Minocycline (MIN)...
December 22, 2016: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28003595/detection-of-measles-virus-genotypes-b3-d4-d5-d8-and-h1-in-the-surveillance-system-in-hokkaido-japan-during-pre-and-post-elimination-era-2006-2015
#11
Masahiro Miyoshi, Rika Komagome, Hiroki Yamaguchi, Asami Ohnishi, Masayuki Kikuchi, Setsuko Ishida, Hideki Nagano, Motohiko Okano
Measles is an acute and highly contagious disease caused by measles virus (MeV). In Japan, since the last epidemic in 2007 and 2008 caused by the genotype D5 strains, the government introduced a catch-up-vaccination program for teenagers during fiscal years of Japan 2008-2012 and the mandatory case-based reporting system for the nationwide elimination. Furthermore, the laboratory confirmation based on genotyping has been performed to clarify the source of infection and to support the interruption of measles cases...
December 22, 2016: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28003594/first-isolation-of-hybrid-shigatoxigenic-and-enterotoxigenic-escherichia-coli-stec-etec-strain-harboring-stx2-and-elt-genes-in-republic-of-korea
#12
Kyung-Hwan Oh, Eunkyung Shin, Su-Mi Jung, Jinsuk Im, Seung-Hak Cho, Sahyun Hong, Cheon-Kwon Yoo, Gyung Tae Chung
No abstract text is available yet for this article.
December 22, 2016: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28003593/dengue-virus-induced-reactive-oxygen-species-production-in-rat-microglia-cells
#13
Lattapon Suwanprinya, Noppawan Phumala Morales, Pimtip Sanvarinda, Hamady Dieng, Tamaki Okabayashi, Ronald Enrique Morales Vargas
Encephalitis has been reported worldwide as a severe complication in patients infected by dengue virus. Reactive oxygen species (ROS) production is a key mechanism involved in the neuronal damage caused by viral encephalitis. Here the capability of the dengue virus serotypes 2 and 4 (DV2 and DV4) to induce ROS production was investigated in a rat microglia cell line, HAPI cells. The cells were infected with DV2 and DV4 at a MOI of 0.1 for a 2-h adsorption period. Japanese encephalitis virus (JEV) was used as the reference...
December 22, 2016: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28003592/antibiotic-susceptibilities-and-genetic-characterization-of-extended-spectrum-beta-lactamase-producing-escherichia-coli-isolated-from-stools-of-pediatric-diarrhea-patients-in-surabaya-indonesia
#14
Eddy Bagus Wasito, Katsumi Shigemura, Kayo Osawa, Alpha Fardah, Akiho Kanaida, Dadik Raharjo, K Kuntaman, Usman Hadi, Sugeng Harijono, Subijanto Marto Sudarmo, Tatsuya Nakamura, Keigo Shibayama, Masato Fujisawa, Toshiro Shirakawa
The purpose of this study was to investigate extended-spectrum beta-lactamase (ESBL)-producing E. coli isolated from pediatric (0-3 year old) diarrhea patients, as well as bacterial antibiotic susceptibilities, ESBL typing, multilocus sequence typing (MLST) typing and diarrheagenic E. coli (DEC)-typing in Surabaya, Indonesia, where this kind of survey is rarely done. ESBL-producing E. coli were detected in 18.8 % of strains. Many ESBL-producing E. coli had significantly less susceptibility to gentamicin (p<0...
December 22, 2016: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28003591/comparison-of-enterohemorrhagic-escherichia-coli-ehec-o157-and-ehec-non-o157-isolates-from-diarrheal-patients-in-the-republic-of-korea
#15
Kyung-Hwan Oh, Su-Mi Jung, Eunkyung Shin, Gyung Tae Chung, Won-Keun Seong, Seung-Hak Cho
We compared 47 enterohemorrhagic Escherichia coli (EHEC) O157 isolates with 184 EHEC non-O157 isolates from Korean patients with diarrhea. In the O157 group, the strains harboring both shiga toxin genes (stx1 and stx2) were detected with highest frequency, whereas the strains harboring only stx1 gene were most frequently detected in the non-O157 group. Eight virulence genes (eaeA, hlyA, ehx, iha, efa1, tir, toxB, and espA ) were found to show a higher frequency of occurrence in the O157 group than in the non-O157 group...
December 22, 2016: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28003590/multicenter-study-of-the-clinical-presentation-of-staphylococcus-lugdunensis-bacteremia-in-japan
#16
Yusuke Ainoda, Nozomi Takeshita, Ryota Hase, Takahiro Mikawa, Naoto Hosokawa, Ichiro Kawamura, Hanako Kurai, Masahiro Abe, Muneyoshi Kimura, Hideki Araoka, Takahiro Fujita, Kyoichi Totsuka, Kazuhisa Mezaki, Noritaka Sekiya, Norio Ohmagari
Staphylococcus lugdunensis (SL) is a highly pathogenic bacterium compared to other coagulase-negative Staphylococcus spp. (CoNS). However, in Japan, data for this pathogen are sparse, and the current prevalence of SL bacteremia is unknown.A prospective multicenter study across five facilities was performed to investigate the prevalence of SL in blood culture specimens. There were 3,284 patients with positive blood cultures, and 2,478 patients had bacteremia. Among patients with bacteremia, 7 patients (0.28%) had SL bacteremia...
December 22, 2016: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28003589/acute-encephalitic-syndrome-in-adults-and-its-correlation-with-cytokine-levels-in-serum-and-cerebrospinal-fluid
#17
Santosh K Singh, Dinkar Kulshreshtha, Ajai K Singh, Pradeep K Maurya, Anup K Thacker
Acute encephalitis syndrome (AES) is a major health problem in developing countries like India. Neuronal injury in encephalitis is attributed to direct toxicity from pathogens and proinflammatory cytokines. In this study, we assessed the serum and cerebrospinal fluid (CSF) levels of cytokines and their correlation with clinical symptoms. Patients with AES of less than 2 weeks duration underwent brain imaging followed by a CSF examination for routine parameters and viral studies. Cytokines IL-6, IL-10 and RANTES levels were assessed in the serum in all patients and in 50 CSF samples and compared with serum cytokine levels in 64 age and sex matched controls...
December 22, 2016: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/27169947/evaluation-of-five-polymerase-chain-reaction-pcr-assays-for-diagnosis-of-the-shiga-toxin-2f-producing-escherichia-coli
#18
Alireza Koochakzadeh, Mahdi Askari Badouei
No abstract text is available yet for this article.
November 22, 2016: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/27000459/non-susceptibility-of-neonatal-and-adult-rats-against-the-middle-east-respiratory-syndrome-coronavirus
#19
Naoko Iwata-Yoshikawa, Shuetsu Fukushi, Aiko Fukuma, Tadaki Suzuki, Makoto Takeda, Masato Tashiro, Hideki Hasegawa, Noriyo Nagata
The present study examined the susceptibility of rats to the Middle East respiratory syndrome coronavirus (MERS-CoV) and determined whether this animal is a suitable model for MERS-CoV infection. Immunohistochemical analysis identified dipeptidyl peptidase 4 (DPP4), a known receptor for MERS-CoV on type I pneumocytes from infected rats. Whereas adult rats developed antibodies against MERS-CoV spike protein after intranasal inoculation, there was no evidence of viral replication in the lungs of adult, young, or neonatal rats after intranasal inoculation with MERS-CoV...
November 22, 2016: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/27000457/accuracy-of-lamp-tb-method-for-diagnosing-tuberculosis-in-haiti
#20
Taijin Kaku, Fujihiko Minamoto, Richard D'Meza, Willy Morose, Jacque Boncy, Josette Bijou, Harry Geffrard, Miki Yoshida, Toru Mori
The procedure of ultra-rapid extraction (PURE) and loop-mediated isothermal amplification for tuberculosis (LAMP-TB) is a simple and rapid manual tuberculosis diagnostic with medium-throughput capability. Because of its simplicity, this method could be useful in resource-limited conditions such as microscopy centers in developing countries. This study was conducted to evaluate the clinical performance of this method in a point-of-care setting. The performance was compared to that of smear microscopy and liquid culture in a hospital laboratory in Haiti, which is considered a representative facility for the implementation of this method...
November 22, 2016: Japanese Journal of Infectious Diseases
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