Read by QxMD icon Read


Xue-Jian Zhang, Xiao-Wei Wang, Jiaxing Sun, Chao Su, Shuguang Yang, Wen-Bin Zhang
Protein immobilization is critical to utilize their unique functions in diverse applications. Herein, we report that orthogonal peptide-protein chemistry enabled multilayer construction can facilitate the incorporation of various folded structural domains, including calmodulin in different states, affibody and dihydrofolate reductase (DHFR). An extended conformation is found to be the most advantageous for steady film growth. The resulting protein thin films exhibit sensitive and selective responsive behaviors to bio-signals (Ca2+, TFP, NADPH, etc...
May 16, 2018: Biomacromolecules
Hyun Su Min, Hyun Jin Kim, Jooyeon Ahn, Mitsuru Naito, Kotaro Hayashi, Kazuko Toh, Beob Soo Kim, Yasuhiro Matsumura, Ick Chan Kwon, Kanjiro Miyata, Kazunori Kataoka
Antibody fragment (Fab')-installed polyion complex (PIC) micelles were constructed to improve targetability of small interfering RNA (siRNA) delivery to pancreatic cancer cells. To this end, we synthesized a block copolymer of azide-functionalized poly(ethylene glycol) (PEG) and poly(L-lysine) (PLL), and prepared PIC micelles with siRNA. Then, a dibenzylcyclooctyne (DBCO)-modified anti-human tissue factor (TF) Fab' was conjugated to azido groups on the micellar surface. A fluorescence correlation spectroscopic (FCS) analysis revealed that 1, 2, or 3 molecule(s) of Fab'(s) were installed onto one micellar nanoparticle according to the feeding ratio of Fab' (or DBCO) to micelle (or azide)...
May 16, 2018: Biomacromolecules
Ana M Carvalho, Raquel Teixeira, Ramon Novoa-Carballal, Ricardo A Pires, Rui L Reis, Iva Pashkuleva
Cancer progression is associated with overexpression of various receptors at the cell surface. Among these, CD44 is known to recognize and bind specifically hyaluronan (HA) and interact with less affinity to other glycosaminoglycans (GAGs), such as chondroitin sulfate (CS). In this study, we describe a simple method to obtain micellar nanoparticles with GAGs shell (HA or CS) as potential drug delivery systems that target cancer cells overexpressing CD44. Alkane thiol was conjugated at the reducing end of the respective GAG using highly efficient oxime chemistry...
May 14, 2018: Biomacromolecules
Sven F Plappert, Sakeena Quraishi, Nicole Pircher, Kirsi S Mikkonen, Stefan Veigel, Karl Michael Klinger, Antje Potthast, Thomas Rosenau, Falk W Liebner
2,3-Dialdehyde cellulose (DAC) of a high degree of oxidation (92% relative to AGU units) prepared by oxidation of microcrystalline cellulose with sodium periodate (48 °C, 19 hours) is soluble in hot water. Solution casting, slow air drying, hot pressing and reinforcement by cellulose nanocrystals afforded films (~100 µm thickness) that feature intriguing properties: they have very smooth surfaces (SEM), are highly flexible and have good light transmittance for both the visible and near-infrared range (89-91%), high tensile strength (81-122 MPa) and modulus of elasticity (3...
May 14, 2018: Biomacromolecules
Xuan Yang, Fredrik Berthold, Lars A Berglund
To expand the use of renewable materials, paper products with superior mechanical and optical properties are needed. Although beating, bleaching and additives are known to improve industrially produced Kraft pulp papers, properties are limited by the quality of the fibers. While the use of nanocellulose has been shown to significantly increase paper properties, the current cost associated with their production has limited their industrial relevance. Here, using a simple mild peracetic acid (PAA) delignification process on spruce, we produce hemicellulose-rich holocellulose fibers (28...
May 14, 2018: Biomacromolecules
Konrad W Klockars, Blaise L Tardy, Maryam Borghei, Anurodh Tripathi, Luiz Guillherme Garcia Greca, Orlando J Rojas
Outstanding optical and mechanical properties can be obtained from hierarchical assemblies of nanoparticles. Herein, the formation of helically-ordered, chiral nematic films obtained from aqueous suspensions of cellulose nanocrystals (CNCs) were studied as a function of the initial suspension state. Specifically, nanoparticle organization and the structural colors displayed by the resultant dry films were investigated as a function of the anisotropic volume fraction (AVF), which depended on the initial CNC concentration and equilibration time...
May 12, 2018: Biomacromolecules
Mathew T Hembury, Nataliia Beztsinna, Hamed Asadi, Joep van den Dikkenberg, Johannes D Meeldijk, Wim E Hennink, Tina Vermonden
Ultrasmall gold atom clusters (< 2 nm in diameter) or gold nanoclusters exhibit emergent photonic properties (near-infrared absorption and emission) compared to larger plasmonic gold particles, due to the significant quantization of their conduction band. While single gold nanoclusters properties and applications are being increasingly investigated, little is still known about their behaviour and properties when assembled into suprastructures and even less studies are investigating their use for biomedical applications...
May 11, 2018: Biomacromolecules
Eda Aydindogan, Emine Guler Celik, Dilek Odaci Demirkol, Shuhei Yamada, Takeshi Endo, Suna Timur, Yusuf Yagci
A novel catechol bearing polypeptide (CtP) was synthesized and used as a component of electrochemical biosensor involving both enzymatic activity and affinity-based sensing systems. Glucose oxidase (GOx) and Anti-Immunoglobulin G (Anti-IgG) were selected as model biorecognition elements for the selective analysis of glucose and IgG. Step by step surface modifications were followed using various techniques such as cyclic voltammetry (CV) and electrochemical impedance spectrometry (EIS) as well as X-Ray photoelectron spectroscopy (XPS)...
May 11, 2018: Biomacromolecules
Lukas Hahn, Michael M Lübtow, Thomas Lorson, Frederik Schmitt, Antje Appelt-Menzel, Rainer Schobert, Robert Luxenhofer
Many natural compounds with interesting biomedical properties share one physicochemical property, namely a low water solubility. Polymer micelles are, among others, a popular means to solubilize hydrophobic compounds. The specific molecular interactions between the polymers and the hydrophobic drugs are diverse and recently it has been discussed that macromolecular engineering can be used to optimize drug loaded micelles. Specifically, π-π stacking between small molecules and polymers has been discussed as an important interaction that can be employed to increase drug loading and formulation stability...
May 10, 2018: Biomacromolecules
Seo Hee Chang, Hyun Jung Lee, Sohee Park, Yelin Kim, Byeongmoon Jeong
Polycaprolactone (PCL) was reported a long time ago; however, its biomedical applications has not been extensively investigated in comparison with poly(lactide-co-glycolide) (PLGA) due to its too slow degradation profile. Here, we are reporting an oxalate-connected oligocaprolactone multiblock copolymer (PCL-OX) as a fast degradable PCL while maintaining its crystalline properties and low melting point of PCL. The in-vivo application of the paclitaxel-loaded PCL-OX microspheres provided a steady plasma drug concentration of 6-9 μg/ml over 28 days, similar to that of the PLGA microspheres...
May 9, 2018: Biomacromolecules
Nibedita Pradhan, Koushik Debnath, Suman Mandal, Nihar R Jana, Nikhil R Jana
Protein aggregation is linked to variety of neurodegenerative disorders and other diseases. Current research involves understanding the mechanism of protein aggregation, inhibiting protein aggregation under intra-/extracellular space, lowering of toxicity arising due to soluble oligomers and augmenting the clearance of protein aggregates from the brain. Toward this direction different types of anti-amyloidogenic small molecules, macromolecules and nanomaterials are identified that can inhibit protein aggregation and extensive progress has been made for their effective utilization...
May 9, 2018: Biomacromolecules
Hao Tang, Jiajing Zhang, Jin Tang, Yi Shen, Wenxuan Guo, Min Zhou, Ruihua Wang, Ni Jiang, Zhihua Gan, Qingsong Yu
Efficient tumor accumulation and body clearance are two paralleled requirement for ideal nanomedicines. However, it's hard for both to be met simultaneously. The inefficient clearance often restrains the application of drug delivery systems (DDSs), especially for high-dosage administration. In this study, the star-like and block structures are combined to enhance the tumor specific targeting of the parent structures and obtain additional renal excretion property. The influences of polymer architectures and chemical compositions on the physicochemical and biological properties, particularly the simultaneous achievement of tumor accumulation and renal clearance have been investigated...
May 9, 2018: Biomacromolecules
Nan Xie, Ke Feng, Jianqun Shao, Bin Chen, Chen-Ho Tung, Li-Zhu Wu
Through modular ROMP (ring-opening metathesis polymerization), biofunctional polynorbornenes are designed and fabricated from panchromatic fluorophores, bioactive peptides and PEG solubilizer for organelle-specific multicolor imaging. Attributed to the free permutation and combination of highly fluorescent red rhodamine B, green dichlorofluorescein and blue 9,10-diphenylanthracene fluorophores as well as signaling peptide sequences of Fx rFx K and TAT, we successfully realize simultaneous multicolor imaging toward lysosomes and mitochondria in living cells firstly utilizing polymeric scaffolds...
May 9, 2018: Biomacromolecules
Nasha Qiu, Jianqing Gao, Qi Liu, Jinqiang Wang, Youqing Shen
Gene therapy has demonstrated effectiveness in many genetic diseases as evidenced by recent clinical applications. Viral vectors have been extensively tested in clinical gene-therapy trials, but nonviral vectors such as cationic polymers or lipids are much less used due to their lower gene-transfection efficiencies. However, the advantages of nonviral vectors, such as easily tailored structures, nonimmunogenetics, and relatively low cost, still drive great efforts to improve their transfection efficiencies...
May 8, 2018: Biomacromolecules
Donghao Wang, Wenyi Ding, Kaiyue Zhou, Shutong Guo, Qiang Zhang, David M Haddleton
Immobilization of enzyme could offer the biocatalyst with increased stability and important recoverability, which plays a vital role for the enzyme's industrial applications. In this study, we present a new strategy to build an intelligent enzyme carrier by coating titania nanoparticles with thermoresponsive epoxy-functionalized polymers. Zero-valent copper mediated living radical polymerization (Cu(0)-LRP) was utilized herein to copolymerize N-isopropylacrylamide (NIPAM) and glycidyl acrylate (GA), directly from an unprotected dopamine-functionalized initiator, in order to obtain an epoxy-containing polymer with terminal anchor for the "grafting to" or "one-pot" modification of titania nanoparticles...
May 8, 2018: Biomacromolecules
Valeria Castelletto, Ruth H Barnes, Kimon-Andreas Karatzas, Charlotte J C Edwards-Gayle, Francesca Greco, Ian W Hamley, Robert Rambo, Jani Seitsonen, Janne Ruokolainen
The activity of antimicrobial peptides stems from their interaction with bacterial membranes, which are disrupted according to a number of proposed mechanisms. Here, we investigate the interaction of a model antimicrobial peptide that contains a single arginine residue with vesicles containing model lipid membranes. The surfactant-like peptide Ala6-Arg (A6R) is studied in the form where both termini are capped (CONH-A6R-NH2, capA6R) or uncapped (NH2-A6R-OH, A6R). Lipid membranes are selected to correspond to model anionic membranes (POPE/POPG) resembling those in bacteria or model zwitterionic membranes (POPC/DOPC) similar to those found in mammalian cells...
May 8, 2018: Biomacromolecules
Sanaullah Khan, Richard Ipsen, Kristoffer Almdal, Birte Svensson, Pernille Harris
The dimeric structure of bovine β-lactoglobulin A (BLGA) at pH 4.0 was solved to 2.0 Å resolution. Fitting the BLGA pH 4.0 structure to SAXS data at low ionic strength (goodness of fit R-factor = 3.6%) verified the dimeric state in solution. Analysis of the monomer-dimer equilibrium at varying pH and ionic strength by SAXS and scattering modeling showed that BLGA is dimeric at pH 3.0 and 4.0, shifting towards a monomer at pH 2.2, 2.6 and 7.0 yielding monomer/dimer ratios of 80/20%, 50/50% and 25/75%, respectively...
May 8, 2018: Biomacromolecules
Jingjing Wei, Hao Meng, Beibei Guo, Zhiyuan Zhong, Fenghua Meng
The ring opening copolymerization of trimethylene carbonate (TMC) and dithiolane trimethylene carbonate (DTC) using acidic and basic organocatalysts, i.e. diphenyl phosphate (DPP) and triazabicyclo[4.4.0]dec-5-ene (TBD), was systemically investigated. Interestingly, DPP and TBD gave rise to completely different polymerization kinetics and copolymer sequences. The copolymerization of TMC and DTC using methoxy poly(ethylene glycol) (mPEG-OH) as an initiator and DPP as a catalyst proceeded in a first-order manner and to near completion in 72 h for both monomers, yielding well-controlled copolymers with random sequences, predictable molar mass and low dispersity (Mw/Mn = 1...
May 7, 2018: Biomacromolecules
Ville Hynninen, Sami Hietala, Jason Robert McKee, Lasse Murtomäki, Orlando J Rojas, Olli Ikkala, Nonappa Nonappa
We show that composite hydrogels comprising methyl cellulose (MC) and cellulose nanocrystal (CNC) colloidal rods display a reversible and enhanced rheological storage modulus and optical birefringence upon heating, i.e., inverse thermoreversibility. Dynamic rheology, quantitative polarized optical microscopy (POM), isothermal titration calorimetry (ITC), circular dichroism (CD), and scanning and transmission electron microscopy (SEM and TEM) were used for characterization. The concentration of CNC in aqueous media was varied up to 3...
May 7, 2018: Biomacromolecules
Bartlomiej Kalaska, Kamil Kamiński, Joanna Miklosz, Keita Nakai, Shin-Ichi Yusa, Dariusz Pawlak, Maria Nowakowska, Andrzej Mogielnicki, Krzysztof Szczubiałka
Di- and triblock copolymers with low dispersity of molecular weight were synthesized using radical addition-fragmentation chain transfer polymerization. The copolymers contained anionic poly(sodium 2-acrylamido-2-methylpropanesulfonate) (PAMPS) block as an anticoagulant component. The block added to lower the toxicity was either poly(ethylene glycol) (PEG) or poly(2-(methacryloyloxy)ethyl phosphorylcholine) (PMPC). The polymers prolonged clotting times both in vitro and in vivo. The influence of the polymer architecture and composition on the efficacy of anticoagulation and safety parameters was evaluated...
May 7, 2018: Biomacromolecules
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"