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Lesan Yan, Joann Miller, Min Yuan, Jessica F Liu, Theresa M Busch, Andrew Tsourkas, Zhiliang Cheng
Photodynamic therapy (PDT) has attracted widespread attention in recent years as a non-invasive and highly selective approach for cancer treatment. We have previously reported a significant increase in the 90-day complete response rate when tumor-bearing mice are treated with the epidermal growth factor receptor (EGFR) inhibitor erlotinib prior to PDT with the photosensitizer benzoporphyrin-derivative monoacid ring A (BPD-MA) compared to treatment with PDT alone. To further explore this strategy for anticancer therapy and clinical practice, we tested whether pre-treatment with erlotinib also exhibited a synergistic therapeutic effect with a nanocarrier containing the clinically relevant photosensitizer protoporphyrin IX (PpIX)...
April 24, 2017: Biomacromolecules
Anouck L S Burzava, Marek Jasieniak, Michaelia P Cockshell, Claudine S Bonder, Frances J Harding, Hans Joerg Griesser, Nicolas H Voelcker
The propensity of glycosaminoglycans to mediate cell-cell and cell-matrix interactions opens the door to capture cells, including circulating blood cells, onto biomaterial substrates. Chondroitin sulfate (CS) -B is of particular interest, since it interacts with the receptor (EGF)-like module-containing mucin-like hormone receptor-like 2 precursor (EMR2) displayed on the surface of leukocytes and endothelial progenitor cells. Herein, CS-B and its isomer CS-A were covalently immobilized onto heptylamine plasma polymer films via three different binding chemistries to develop platform technology for the capture of EMR2 expressing cells onto solid carriers...
April 24, 2017: Biomacromolecules
Maija Vuoriluoto, Hannes Orelma, Meri Johanna Lundahl, Maryam Borghei, Orlando J Rojas
We demonstrate benzophenone (BP) conjugation via amine-reactive esters onto oxidized cellulosic fibers that were used as precursors, after microfluidization, of photo-active cellulose nanofibrils (CNF). From these fibrils, cellulose I filaments were synthesized by hydrogel spinning in an anti-solvent followed by fast bi-radical UV crosslinking. As a result, the BP-CNF filaments developed a wet tensile strength of up to ~105 MPa (a remarkable retention of ~80% of the dry strength). Thus, the principal limitation of these emerging materials was resolved (< 0...
April 24, 2017: Biomacromolecules
Tomas Majtan, Insun Park, Jan P Kraus
Homocystinuria due to loss of cystathionine beta-synthase (CBS) causes accumulation of homocysteine and depletion of cysteine. Current treatments are suboptimal and thus the development of an enzyme replacement therapy based on PEGylated human truncated CBS (PEG-CBS) has been initiated. Attenuation of potency was observed, necessitating a screen of several PEG-CBS conjugates for their efficacy to correct and maintain the plasma metabolite profile of murine homocystinuria after repeated administrations interrupted with washouts...
April 21, 2017: Biomacromolecules
Kuisma Littunen, Galina Mai-Gisondi, Jukka Seppälä, Emma R Master
Wheat arabinoxylan was treated with two α-arabinofuranosidases exhibiting different mode of action to create three different polymeric substrates. These three substrate preparations were characterized by xylopyranose backbone sugars that are (1) singly substituted by arabinose at C2 or C3, (2) doubly substituted by arabinose at C2 and C3, and (3) largely unsubstituted. All xylan preparations were grafted with glycidyl methacrylate using cerium ammonium nitrate and then evaluated in terms of graft yield and adsorption to cellulose surfaces...
April 21, 2017: Biomacromolecules
Weihua Wang, Li Li, Katja Henzler, Yan Lu, Junyou Wang, Haoya Han, Yuchuan Tian, Yunwei Wang, Zhiming Zhou, Gudrun Lotze, Theyencheri Narayanan, Matthias Ballauff, Xuhong Guo
The immobilization of bovine serum albumins (BSA) onto cationic spherical polyelectrolyte brushes (SPB) consisting of a solid polystyrene (PS) core and a densely grafted poly(2-aminoethyl methacrylate hydrochloride) (PAEMH) shell was studied by small-angle X-ray scattering (SAXS). The observed dynamics of adsorption of BSA onto SPB by time-resolved SAXS can be divided into two stages. In the first stage (tens of milliseconds), the added proteins as in-between bridge instantaneously caused the aggregation of SPB...
April 20, 2017: Biomacromolecules
Meryem O Pehlivaner Kara, Adam K Ekenseair
The development of synthetic and well-defined extracellular matrices that are free of xenogeneic components and are capable of inducing desired cellular responses holds great potential for use in vitro as 3D cell culture environments and in vivo as scaffolds for tissue regeneration. In this study, the impact of free and partially occupied epoxide groups on the viability, activity, and behavior of rat fibroblasts encapsulated in thermoresponsive hydrogels based on poly(N-isopropylacrylamide) (pNiPAAm) was investigated...
April 20, 2017: Biomacromolecules
Ivaylo Stefanov, Sílvia Pérez-Rafael, Javier Hoyo, Jonathan Cailloux, Orlando O Santana Perez, Dolores Hinojosa-Caballero, Tzanko Tzanov
The healing of chronic wounds requires intensive medical intervention at huge healthcare costs. Dressing materials should consider the multifactorial nature of these wounds comprising deleterious proteolytic and oxidative enzymes, and high bacterial load. In this work, multifunctional hydrogels for chronic wound application were produced by enzymatic crosslinking of thiolated chitosan and gallic acid. The hydrogels combine several beneficial to wound healing properties, controlling the matrix metalloproteinases (MMPs) and myeloperoxidase (MPO) activities, oxidative stress and bacterial contamination...
April 19, 2017: Biomacromolecules
Patrick Gilles, Kirstin Wenck, Inga Stratmann, Michael Kirsch, Daniel A Smolin, Torsten Schaller, Herbert de Groot, Arno Kraft, Thomas Schrader
This account presents a general method for the construction of polymeric surface binders for digestion enzymes. Two prominent parts, namely the modification of the copolymer composition and the screening assay for the most powerful inhibitors are both amenable to parallelization. The concept hinges on the appropriate selection of amino-acid-selective comonomers, their free radical copolymerization, and subsequent screening of the resulting copolymer library for efficient enzyme inhibition. A microscale synthetic procedure for the copolymerization process was developed, which produces water-soluble affinity polymers that can be stored for years at room temperature...
April 18, 2017: Biomacromolecules
Joji Tanaka, Anne S Gleinich, Qiang Zhang, Richard Whitfield, Kristian Kempe, David M Haddleton, Thomas P Davis, Sébastien Perrier, Daniel A Mitchell, Paul Wilson
A range of glycopolymers composed of N-acetylgalactosamine were prepared via sequential Cu(I)-mediated polymerization and alkyne-azide click (CuAAC). The resulting polymers were shown, via multichannel surface plasmon resonance, to interact specifically with human macrophage galactose lectin (MGL; CD301) with high affinity (KD = 1.11 μM), but they did not bind to the mannose/fucose-selective human lectin dendritic-cell-specific intercellular adhesion molecule-3-grabbing nonintegrin (DC-SIGN; CD209). The effect of sugar ligand valency on the binding (so-called "glycoside cluster effect") of poly(N-acetylgalactosamine) to MGL was investigated by varying first the polymer chain length (DP: 100, 64, 40, 23, 12) and then the architecture (4- and 8-arm star glycopolymers)...
April 18, 2017: Biomacromolecules
Alberto Gallardo, Enrique Martínez-Campos, Carolina García, Aitziber L Cortajarena, Juan Rodríguez-Hernández
In this manuscript, we describe the fabrication of hydrogel supports for mammalian cell handling that can simultaneously prevent materials from microbial contamination and therefore allow storage in aqueous media. For that purpose, hydrogels based on the antifouling polymer polyvinylpyrrolidone (PVP) were functionalized with different ionic groups (anionic, cationic, or two types of zwitterions). In order to prevent bacterial adhesion in the long-term, we took advantage of the synergistic effect of inherently antifouling PVP and additional antifouling moieties incorporated within the hydrogel structure...
April 18, 2017: Biomacromolecules
Maciej Studzian, Aleksandra Szulc, Anna Janaszewska, Dietmar Appelhans, Łukasz Pułaski, Barbara Klajnert-Maculewicz
Poly(propylene imine) dendrimers of 4th generation partially modified with maltose (open shell structure, PPI-m OS) have been proposed as carriers for nucleotide anticancer drugs. The aim of this work was to provide basic insight into interactions between fluorescently labeled PPI-m dendrimer and two distinct leukemia cell models: CCRF-1301 lymphoid cell line and HL-60 myeloid cell line. We applied qualitative confocal imaging and quantitative flow cytometry, as well as trypan blue quenching and pharmacological inhibition, to investigate the course, kinetics and molecular mechanisms of internalization of nanoparticles...
April 17, 2017: Biomacromolecules
Claudia Battistella, Harm-Anton Klok
Inhibition of P-glycoprotein (P-gp) transporter is an attractive approach for the reversion of cancer-associated multidrug resistance (MDR). PHPMA-based carriers that are able to release the anticancer drug doxorubicin in the lysosomes have shown promise to reduce P-gp mediated resistance. This is attributed to the release of the drug in close proximity to the nucleus and distant from the P-gp transporter. This work presents a strategy to maximize P-gp inhibition and enhance doxorubicin cytotoxicity in cancer cells by using a dual functional PHPMA conjugate carrying both the anticancer drug doxorubicin and the P-glycoprotein inhibitor zosuquidar (Zos)...
April 14, 2017: Biomacromolecules
Carlos Caro, María Luisa García-Martín, Manuel Pernia Leal
pH-responsive nanogels (NGs) were used to prepare high-efficiency magnetic resonance imaging dual T1/T2 contrast agents for pH imaging. The polymeric NG matrix acts as a strong polydentate ligand that chelates the Mn cations in its inner cavity generating a hybrid NG structure. The Mn chelate NG is sensitive to pH changes, such that protonation induces a change of the polymer hydration state and consequent swelling. The swollen nanogel allows water molecules to enter and interact with the Mn chelate, shortening the relaxation time (switch ON) and giving rise to positive or negative contrast on T1- or T2-weighted magnetic resonance images...
April 14, 2017: Biomacromolecules
Katsiaryna Prudnikova, Robert W Yucha, Pavan Patel, Alicia Kriete, Lin Han, Lynn S Penn, Michele S Marcolongo
Aging and degeneration of human tissue come with the loss of tissue water retention and associated changes in physical properties partially due to degradation and subsequent loss of proteoglycans. We demonstrated a novel method of fabrication of biomimetic proteoglycans, which mimic the three-dimensional bottlebrush architecture and physical behavior of natural proteoglycans responsible for tissue hydration and structural integrity. Biomimetic proteoglycans are synthesized by an end-on attachment of natural chondroitin sulfate bristles to a synthetic poly(acryloyl chloride) backbone...
April 11, 2017: Biomacromolecules
Ian W Hamley, Samuel Burholt, Jessica Hutchinson, Valeria Castelletto, Emerson Rodrigo da Silva, Wendel Alves, Philipp Gutfreund, Lionel Porcar, Rajeev Dattani, Daniel Hermida-Merino, Gemma Newby, Mehedi Reza, Janne Ruokolainen, Joanna Stasiak
No abstract text is available yet for this article.
April 10, 2017: Biomacromolecules
Kazuhiro Sato, Shingo Kobayashi, Asuka Sekishita, Miyuki Wakui, Masaru Tanaka
A poly(vinyl acetate) derivative, poly(3-methoxypropionic acid vinyl ester) (PMePVE), was synthesized to develop a new candidate for blood compatible polymers. The monomer MePVE was synthesized by a simple 2-step reaction, and then, the MePVE was polymerized via free radical polymerization to obtain PMePVE. Human platelet adhesion tests were performed to evaluate the thrombogenicity, and the platelet adhesion was suppressed on the PMePVE-coated substrate. To determine the expression of the non-thrombogenicity of the PMePVE, the plasma protein adsorption and a conformationally altered state of fibrinogen were analyzed by a microBCA assay and ELISA...
April 10, 2017: Biomacromolecules
Andrew R Swartz, Qing Sun, Wilfred Chen
Affinity precipitation is an ideal alternative to chromatography for antibody purification because it combines the high selectivity of an affinity ligand with the operational benefits of precipitation. However, the widespread use of elastin-like polypeptide (ELP) capture scaffolds for antibody purification has been hindered by the high salt concentrations and temperatures necessary for efficient ELP aggregation. In this paper, we employed a tandem approach to enhance ELP aggregation by enlarging the dimension of the capturing scaffold and by creating IgG-triggered scaffold cross-linking...
April 10, 2017: Biomacromolecules
Alejandro J Benítez, Andreas Walther
Nanopapers formed from aqueous dispersions of cellulose nanofibrils (CNFs) combine stiffness, strength, and toughness. Yet, delicate interactions operate between the CNFs during nanopaper formation and mechanical deformation. We unravel in detail how counterions, being either of the organic alkyl ammonium kind (NR4(+)) or of the earth metal series (Li(+), Na(+), Cs(+)), need to be chosen to achieve outstanding combinations of stiffness, strength, and toughness, extending to previously unreached territories...
April 5, 2017: Biomacromolecules
Adel Abouhmad, Tarek Dishisha, Magdy A Amin, Rajni Hatti-Kaul
Antibacterial bionanostructures were produced from cellulose nanocrystals (CNC) with immobilized lysozyme from hen egg white (HEW) and T4 bacteriophage, respectively. The nanocrystals were prepared from microcrystalline cellulose by ammonium persulfate oxidation with a yield of 68% and having an average size of 250 nm and low polydispersity index. HEW lysozyme (HEWL) and T4 lysozyme (T4L) were immobilized to CNC by different mechanisms including adsorption and covalent coupling to carbodiimide-activated carboxylate groups and to glutaraldehyde-activated aminated CNC (Am-CNC), respectively...
April 5, 2017: Biomacromolecules
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