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Nucleosides, Nucleotides & Nucleic Acids

Mamta Tripathi, Chandra Gopal Giri, Devashish Das, Rama Pande, Sougata Sarkar, Santanab Giri, Gourisankar Roymahapatra, Avijit Sarkar
Azo linked salicyldehyde and a new 2-hydroxy acetophenone based ligands (HL1 and HL2 ) with their copper(II) complexes [Cu(L1 )2 ] (1) and [Cu(L2 )2 ] (2) were synthesized and characterized by spectroscopic methods such as 1 H, 13C NMR, UV-Vis spectroscopy and elemental analyses. Calculation based on Density Functional Theory (DFT), have been performed to obtain optimized structures. Binding studies of these copper (II) complexes with calf thymus DNA (ct-DNA) and torula yeast RNA (t-RNA) were analyzed by absorption spectra, emission spectra and Viscosity studies and Molecular Docking techniques...
November 13, 2018: Nucleosides, Nucleotides & Nucleic Acids
Bart A W Jacobs, Dick Pluim, Pia van der Laan, Anna Tzani, Jos H Beijnen, Jan H M Schellens
The enzyme thymidine phosphorylase (TP) is important for activation of capecitabine and 5-fluorouracil. Assessment of TP phenotype might be suitable for identification of patients at risk of fluoropyrimidine-induced toxicity. In this paper, we describe the development and validation an assay for TP activity in peripheral blood mononuclear cells (PBMCs). The assay was based on ex vivo conversion of the TP substrate thymidine to thymine. The amount of thymine formed was determined by high-performance liquid chromatography - ultraviolet detection (HPLC-UV) with 5-bromouracil as internal standard...
October 4, 2018: Nucleosides, Nucleotides & Nucleic Acids
Miral V Lunagariya, Khyati P Thakor, Bharat H Pursuwani, Mohan N Patel
Square planar mononuclear platinum(II) complexes having general formula [Pt(Ln )Cl2 ], (where, Ln  = L1-4 ) were synthesized with neutral bidentate heterocyclic 1,3,5-trisubstituted bipyrazole based ligands. The synthesized compounds were characterized by physicochemical method such as TGA, molar conductance, micro-elemental analysis and magnetic moment, and spectroscopic method such as, FT-IR, UV-vis, 1 H NMR, 13 C NMR and mass spectrometry. Biological applications of the compounds were carried out using in vitro brine shrimp lethality bioassay, in vitro antimicrobial study against five different pathogens, and cellular level cytotoxicity against Schizosaccharomyces pombe (S...
September 19, 2018: Nucleosides, Nucleotides & Nucleic Acids
Muhammad Murtaza Hassan, Ayat Yaseen, Abdelaziz Ebead, Gerald Audette, Edward Lee-Ruff
A synthesis of cyclobutene nucleoside analogs in which the nucleobase is tethered by a methylene group is described. The coupling of 6-chloropurine with 3-hydroxymethyl-cyclobutanone proceeds via its triflate to give both N-7 and N-9 regioisomers with relative yields corresponding to the calculated charge distribution of the 6-chloropurinyl anion. The stereoselective reduction of the N-alkylated ketones yielded quantitatively one stereoisomer in each case. The structural assignments were based on spectroscopic data and single crystal X-ray diffraction...
September 6, 2018: Nucleosides, Nucleotides & Nucleic Acids
Devappa S Lamani, S G Badiger, K R Venugopala Reddy, H S Bhojya Naik
The present paper deals with the synthesis of novel macrocyclic complexes of the type [MLX]X, where [(M = Co(II) (1), and Ni(II) (2) X = (Cl2 )]. The complexes are synthesized by the reaction of ligand(L)diquinolineno[1,3,7,9]tetraazacyclododecine-7,15-ethane(14H,16H)-benzene with the corresponding metal salts. The synthesized complexes are thoroughly characterized by elemental analysis, FT-IR, 1 H-NMR, Mass and electronic spectra. The complexes (1) and (2) were evaluated for in vitro cytotoxicity against human breast adenocarcinoma cell (MCF-7)...
September 6, 2018: Nucleosides, Nucleotides & Nucleic Acids
Vorasit Vongsutilers, Kulwadee Sawaspaiboontawee, Bodin Tuesuwan, Yoko Shinohara, Gota Kawai
Attempting to elucidate biological significance of the left-handed Z-DNA is a research challenge due to Z-DNA potential role in many diseases. Discovery of Z-DNA binding proteins has ignited the interest in search for Z-DNA functions. Biosensor with Z-DNA forming probe can be useful to study the interaction between Z-DNA conformation and Z-DNA binding proteins. In this study, 5-methylcytosine (m C) containing CG decamers were characterized for their suitability to form Z-DNA and to be used in Z-DNA forming probe...
September 6, 2018: Nucleosides, Nucleotides & Nucleic Acids
Jingjing Li, Yi-Ping Liu
Peroxisome proliferator-activated receptors (PPARs), as members of nuclear hormone receptor superfamily, can be activated by binding natural or synthetic ligands. The use of related ligands has revealed many potential roles for PPARs in the pathogenesis of some human metabolic disorders and inflammatory-related disease. Based on the previous studies, this review primarily concluded the current progress of knowledge regarding the specific biological activity of PPARs in cancers, atherosclerosis, and type 2 diabetes mellitus, providing a foundation for the potential therapeutic use of PPAR ligands in human diseases...
July 23, 2018: Nucleosides, Nucleotides & Nucleic Acids
Yoshikazu Hattori, Daichi Yamanaka, Saori Morioka, Taishi Yamaguchi, Honoka Tomonari, Chojiro Kojima, Yoshiyuki Tanaka
Hammerhead ribozymes are a model system for studying molecular mechanism of RNA catalysis. Physicochemical data-driven mechanistic studies are an indispensable step towards understanding the catalysis of hammerhead ribozymes. Here we characterized a model RNA duplex with catalytically important sheared-type G12-A9 base pair and A9-G10.1 metal ion-binding motif in hammerhead ribozymes. By using high magnetic field NMR, all base proton signals, including catalytic residues, were unambiguously assigned. We further characterized structural features of this RNA molecule and found that it reflects the structural features of the A9-G10...
June 28, 2018: Nucleosides, Nucleotides & Nucleic Acids
N Yamada, C Saito, Y Murayama-Chiba, H Kano, Y Asami, H Itoh
Excessive intake of purine-rich foods elevates serum uric acid levels, making it a risk factor for hyperuricemia. We hypothesized that lactic acid bacteria ingested with food might utilize purines and contribute to their decreased absorption in the intestines, thereby preventing hyperuricemia. We previously reported that Lactobacillus gasseri PA-3 (PA-3) incorporates adenosine/inosine and related purines and that oral ingestion of PA-3 reduced the absorption of these purines in rats. However, it is unclear whether PA-3 also decreases the absorption of other purines, such as guanosine 5'-monophosphate (GMP) and guanosine...
May 3, 2018: Nucleosides, Nucleotides & Nucleic Acids
T Fukuuchi, N Iyama, N Yamaoka, K Kaneko
Ribonucleotide flavor enhancers such as inosine monophosphate (IMP) and guanosine monophosphate (GMP) provide umami taste, similarly to glutamine. Japanese cuisine frequently uses soup stocks containing these nucleotides to enhance umami. We quantified 18 types of purines (nucleotides, nucleosides, and purine bases) in three soup stocks (chicken, consommé, and dried bonito soup). IMP was the most abundant purine in all umami soup stocks, followed by hypoxanthine, inosine, and GMP. The IMP content of dried bonito soup was the highest of the three soup stocks...
April 13, 2018: Nucleosides, Nucleotides & Nucleic Acids
Rosa J Torres, Juan G Puig
The neurological manifestations of Lesch-Nyhan disease (LND) have been attributed to the effect of hypoxanthine-guanine phosphoribosyltransferase (HPRT) deficiency on nervous system development. An increase has been reported in the levels of 5-aminoimidazole-4-carboxamide-1-β-D-ribotide (AICAR) and its triphosphate form ZTP in the red blood cells of patients with LND. AICAR accumulation in the brain has been hypothesized as the cause of some of the neurological symptoms of patients with LND. In this study, we examined the effect of AICAR on the differentiation of neurons in the well-established human NTERA-2 cl...
April 10, 2018: Nucleosides, Nucleotides & Nucleic Acids
Jeremy E B McCallum, Christopher W Coyle, Ryan R Elson, Blake A Titterington
The development of small molecules to stabilize the G-quadruplex structure has garnered significant attention for anticancer drug discovery. Herein, we report the synthesis of several 4,4'-diaminoazobenzene derivatives containing different substituent groups and their ability to bind and stabilize telomeric G-quadruplex DNA. Circular dichroism (CD) spectroscopy was performed to characterize the quadruplex topologies, measure stabilization effects, and evaluate their capabilities for conformational photoregulation...
March 12, 2018: Nucleosides, Nucleotides & Nucleic Acids
Galal H Elgemeie, Nahed M Fathy, Ayman B Farag, Sheikha A Alkhursani
A novel series of acyclic pyridine thioglycosides has been synthesized. Evaluation of the anti proliferative activity of these compounds against HEPG-2 cell lines (liver carcinoma cell lines) shows that most of the compounds have high anti-tumor activities especially 6b, 6c, 7b and 7c. Furthermore, in the modeling study, these compounds showed that they have high binding affinity with thymidylate synthase dihydrofolate reductase (TS-DHFR).
March 4, 2018: Nucleosides, Nucleotides & Nucleic Acids
Fida Muhammad Khan, Kainaat William, Samreen Aruge, Safia Janjua, Safdar Ali Shah
Illegal trade is a major threat to the biodiversity and the efforts initiated for the conservation of wildlife. The shortcomings of the traditional taxonomic identification methods have been coped by a revolutionary and emerging technique, the "DNA (Deoxyribonucleic Acid) barcoding". Here we report a case of trader who was allegedly making footwear for a famous international celebrity from wild animal cutis. The samples confiscated during a raid on a footwear manufacturing industry by KP Wildlife department in August, 2016, were received by Bioresource Research Centre (BRC) for molecular identification on 1st September, 2016...
March 4, 2018: Nucleosides, Nucleotides & Nucleic Acids
Nahid Shahabadi, Farshad Shiri, Saba Hadidi
The interaction between the dimer structure of ibuprofen drug (D-IB) and calf thymus DNA under simulative physiological conditions was investigated with the use of Hoechst 33258 and methylene blue dye as spectral probes by the methods of UV-visible absorption, fluorescence spectroscopy, circular dichroism spectroscopy and molecular modeling study.Using the Job's plot, a single class of binding sites for theD-IB on DNA was put in evidence. The Stern-Volmer analysis of fluorescence quenching data shows the presence of both the static and dynamic quenching mechanisms...
March 4, 2018: Nucleosides, Nucleotides & Nucleic Acids
Nahid Shahabadi, Farshad Shiri, Mohammadsaleh Norouzibazaz, Aliyar Falah
The interaction between ibuprofen-Zn(II) complex and calf thymus DNA in physiological buffer (pH 7.4) was studied with the use of Hoechst 33258 and methylene blue dyes as spectral probes by multi-spectroscopic techniques, and viscosity measurements. It was found that ibuprofen-Zn(II) complex molecules could bind with DNA via groove binding mode as evidenced by: i- DNA binding constant (Kb = (1.00 ± 0.2) × 104 M-1 ) from Spectrophotometric studies of the interaction of ibuprofen-Zn(II) complex with DNA is comparable to groove binding drugs...
February 20, 2018: Nucleosides, Nucleotides & Nucleic Acids
Ilya S Dovydenko, Maxim S Kupryushkin, Dmitrii V Pyshnyi, Evgeny K Apartsin
This paper explores the potential of a modified phosphotriester approach to the synthesis of 5'-phosphoramidate derivatives of DNA and RNA oligonucleotides. The modification of 5'-deprotected support-bound oligonucleotides is done in two steps: i) conversion of the 5'-OH group of an oligonucleotide into an activated phosphodiester, and ii) treatment of the activated phosphodiester with an aminocompound. The approach is efficient and compatible with conventional solid phase oligonucleotide synthesis. It can be used for the conjugation of therapeutically relevant oligonucleotides with functional moieties or carrier constructions, which are to be removed after endocytosis...
February 1, 2018: Nucleosides, Nucleotides & Nucleic Acids
Galal H Elgemeie, Mamdouh A Abu-Zaied, Galal A Nawwar
This study reports a novel and efficient method for the synthesis of the first reported novel class of triazole thioglycosides. These series of compounds were designed through the reaction of potassium cyanocarbonimidodithioate 2 with hydrazine derivatives 3a-d in EtOH at room temperature to give the corresponding potassium 5-amino-1H-1,2,4-triazole-3-thiolates 4a-d. The latter compounds were treated with tetra-O-acetyl-α-D-glucopyranosyl bromide 6a and tetra-O-acetyl-α-D-galactopyranosyl bromide 6b in DMF at room temperature to give in high yields the corresponding triazole thioglycosides 7a-h...
February 1, 2018: Nucleosides, Nucleotides & Nucleic Acids
Alicja Stachelska-Wierzchowska, Jacek Wierzchowski, Agnieszka Bzowska, Beata Wielgus-Kutrowska
The title compound is an excellent substrate for E. coli PNP, as well as for its D204N mutant. The main product of the synthetic reaction is N9-riboside, but some amount of N7-riboside is also present. Surprisingly, 1,N6 -ethenoadenine is also ribosylated by both wild-type and mutated (N243D) forms of calf PNP, which catalyze the synthesis of a different riboside, tentatively identified as N6-β-D-ribosyl-1,N6 -ethenoadenine. All ribosides are susceptible to phosphorolysis by the E. coli PNP (wild type). All the ribosides are fluorescent and can be utilized as analytical probes...
February 1, 2018: Nucleosides, Nucleotides & Nucleic Acids
Xiu-An Zheng, Rui Wang, Shan-Shan Gong, Rui Kong, Rong Liu, Qi Sun
An efficient five-step synthetic route for multigram-scale preparation of formylglycinamide ribonucleotide (FGAR) from peracetylated β-d-ribofuranosyl azide has been developed.
February 1, 2018: Nucleosides, Nucleotides & Nucleic Acids
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