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Marine Biotechnology

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https://www.readbyqxmd.com/read/29344826/phytochemical-analysis-and-evaluation-of-the-antioxidant-anti-inflammatory-and-antinociceptive-potential-of-phlorotannin-rich-fractions-from-three-mediterranean-brown-seaweeds
#1
Amal Abdelhamid, Meriem Jouini, Haifa Bel Haj Amor, Zeineb Mzoughi, Mehdi Dridi, Rafik Ben Said, Abderrahman Bouraoui
Phlorotannins, phenolic compounds produced exclusively by seaweeds, have been reported to possess various pharmacological properties. However, there have been few works on these compounds from Mediterranean seaweeds. In this study, we investigated the phytochemical analysis and pharmacological potential of phlorotannin-rich fractions from three brown seaweeds collected along the Tunisia coast: Cystoseira sedoides (PHT-SED), Cladostephus spongeosis (PHT-CLAD), and Padina pavonica (PHT-PAD). Phytochemical determinations showed considerable differences in total phenolic content (TPC) and phlorotannin content (PHT)...
January 18, 2018: Marine Biotechnology
https://www.readbyqxmd.com/read/29344825/the-voltage-dependent-anion-channel-vdac-of-pacific-oysters-crassostrea-gigas-is-upaccumulated-during-infection-by-the-ostreid-herpesvirus-1-oshv-1-an-indicator-of-the-warburg-effect
#2
Lizenn Delisle, Marine Fuhrmann, Claudie Quéré, Marianna Pauletto, Vianney Pichereau, Fabrice Pernet, Charlotte Corporeau
Voltage-dependent anion channel (VDAC) is a key mitochondrial protein. VDAC drives cellular energy metabolism by controlling the influx and efflux of metabolites and ions through the mitochondrial membrane, playing a role in its permeabilization. This protein exerts a pivotal role during the white spot syndrome virus (WSSV) infection in shrimp, through its involvement in a particular metabolism that plays in favor of the virus, the Warburg effect. The Warburg effect corresponds to an atypical metabolic shift toward an aerobic glycolysis that provides energy for rapid cell division and resistance to apoptosis...
January 17, 2018: Marine Biotechnology
https://www.readbyqxmd.com/read/29340846/novel-isoforms-of-n16-and-n19-families-implicated-for-the-nacreous-layer-formation-in-the-pearl-oyster-pinctada-fucata
#3
Fumito Ohmori, Shigeharu Kinoshita, Daisuke Funabara, Hiroki Koyama, Kiyohito Nagai, Kaoru Maeyama, Kikuhiko Okamoto, Shuichi Asakawa, Shugo Watabe
Although a wide variety of proteins and genes possibly related to the shell formation in bivalve have been identified, their functions have been only partially approved. We have recently performed deep sequencing of expressed sequence tags (ESTs) from the pearl oyster Pinctada fucata using a next-generation sequencer, identifying a dozen of novel gene candidates which are possibly associated with the nacreous layer formation. Among the ESTs, we focused on three novel isoforms (N16-6, N16-7, and N19-2) of N16 and N19 families with reference to five known genes in the families and determined the full-length cDNA sequences of these isoforms...
January 16, 2018: Marine Biotechnology
https://www.readbyqxmd.com/read/29330710/glycogen-production-in-marine-cyanobacterial-strain-synechococcus-sp-nkbg-15041c
#4
Amr Badary, Shouhei Takamatsu, Mitsuharu Nakajima, Stefano Ferri, Peter Lindblad, Koji Sode
An important feature offered by marine cyanobacterial strains over freshwater strains is the capacity to grow in seawater, replacing the need for often-limited freshwater. However, there are only limited numbers of marine cyanobacteria that are available for genetic manipulation and bioprocess applications. The marine unicellular cyanobacteria Synechococcus sp. strain NKBG 15041c (NKBG15041c) has been extensively studied. Recombinant DNA technologies are available for this strain, and its genomic information has been elucidated...
January 12, 2018: Marine Biotechnology
https://www.readbyqxmd.com/read/29318417/identifying-a-major-qtl-associated-with-salinity-tolerance-in-nile-tilapia-using-qtl-seq
#5
Xiao Hui Gu, Dan Li Jiang, Yan Huang, Bi Jun Li, Chao Hao Chen, Hao Ran Lin, Jun Hong Xia
Selection of new lines with high salinity tolerance allows for economically feasible production of tilapias in brackish water areas. Mapping QTLs and identifying the markers linked to salinity-tolerant traits are the first steps in the improvement of the tolerance in tilapia through marker-assisted selection techniques. By using QTL-seq strategy and linkage-based analysis, two significant QTL intervals (chrLG4 and chrLG18) on salinity-tolerant traits were firstly identified in the Nile tilapia. Fine mapping with microsatellite and SNP markers suggested a major QTL region that located at 23...
January 9, 2018: Marine Biotechnology
https://www.readbyqxmd.com/read/29313151/cnidarian-primary-cell-culture-as-a-tool-to-investigate-the-effect-of-thermal-stress-at-cellular-level
#6
P Ventura, G Toullec, C Fricano, L Chapron, V Meunier, E Röttinger, P Furla, S Barnay-Verdier
In the context of global change, symbiotic cnidarians are largely affected by seawater temperature elevation leading to symbiosis breakdown. This process, also called bleaching, is triggered by the dysfunction of the symbiont photosystems causing an oxidative stress and cell death to both symbiont and host cells. In our study, we wanted to elucidate the intrinsic capacity of isolated animal cells to deal with thermal stress in the absence of symbiont. In that aim, we have characterized an animal primary cell culture form regenerating tentacles of the temperate sea anemone Anemonia viridis...
January 8, 2018: Marine Biotechnology
https://www.readbyqxmd.com/read/29256104/population-genomics-reveals-genetic-divergence-and-adaptive-differentiation-of-chinese-sea-bass-lateolabrax-maculatus
#7
Yunfeng Zhao, Wenzhu Peng, Huayang Guo, Baohua Chen, Zhixiong Zhou, Jian Xu, Dianchang Zhang, Peng Xu
The marine species usually show high dispersal capabilities accompanied by high levels of gene flow. On the other hand, many physical barriers distribute along the continental marginal seas and may prevent dispersals and increase population divergence. These complexities along the continental margin generate serious challenges to population genetic studies of marine species. Chinese sea bass Lateolabrax maculatus distributes broad latitudinal gradient spanning from the tropical to the mid-temperate zones in the continental margin seas of the Northwest Pacific Ocean...
December 18, 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/29209860/identification-of-inhibitory-compounds-against-singapore-grouper-iridovirus-infection-by-cell-viability-based-screening-assay-and-droplet-digital-pcr
#8
Kuntong Jia, Yongming Yuan, Wei Liu, Lan Liu, Qiwei Qin, Meisheng Yi
Singapore grouper iridovirus (SGIV) is one of the major causative agents of fish diseases and has caused significant economic losses in the aquaculture industry. There is currently no commercial vaccine or effective antiviral treatment against SGIV infection. Annually, an increasing number of small molecule compounds from various sources have been produced, and many are proved to be potential inhibitors against viruses. Here, a high-throughput in vitro cell viability-based screening assay was developed to identify antiviral compounds against SGIV using the luminescent-based CellTiter-Glo reagent in cultured grouper spleen cells by quantificational measurement of the cytopathic effects induced by SGIV infection...
December 5, 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/29204906/mapping-qtl-for-omega-3-content-in-hybrid-saline-tilapia
#9
Grace Lin, Le Wang, Si Te Ngoh, Lianghui Ji, Laszlo Orbán, Gen Hua Yue
Tilapia is one of most important foodfish species. The low omega-3 to omega-6 fatty acid ratio in freshwater tilapia meat is disadvantageous for human health. Increasing omega-3 content is an important task in breeding to increase the nutritional value of tilapia. However, conventional breeding to increase omega-3 content is difficult and slow. To accelerate the increase of omega-3 through marker-assisted selection (MAS), we conducted QTL mapping for fatty acid contents and profiles in a F2 family of saline tilapia generated by crossing red tilapia and Mozambique tilapia...
December 4, 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/29164355/plastid-genome-of-dictyopteris-divaricata-dictyotales-phaeophyceae-understanding-the-evolution-of-plastid-genomes-in-brown-algae
#10
Feng Liu, Zhe Jin, Yu Wang, Yuping Bi, James T Melton
Dictyotophycidae is a subclass of brown algae containing 395 species that are distributed worldwide. A complete plastid (chloroplast) genome (ptDNA or cpDNA) had not previously been sequenced from this group. In this study, the complete plastid genome of Dictyopteris divaricata (Okamura) Okamura (Dictyotales, Phaeophyceae) was characterized and compared to other brown algal ptDNAs. This plastid genome was 126,099 bp in size with two inverted repeats (IRs) of 6026 bp. The D. divaricata IRs contained rpl21, making its IRs larger than representatives from the orders Fucales and Laminariales, but was smaller than that from Ectocarpales...
November 21, 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/29152671/comparative-transcriptome-analysis-provides-insights-into-differentially-expressed-genes-and-long-non-coding-rnas-between-ovary-and-testis-of-the-mud-crab-scylla-paramamosain
#11
Xiaolong Yang, Mhd Ikhwanuddin, Xincang Li, Fan Lin, Qingyang Wu, Yueling Zhang, Cuihong You, Wenhua Liu, Yinwei Cheng, Xi Shi, Shuqi Wang, Hongyu Ma
The molecular mechanism underlying sex determination and gonadal differentiation of the mud crab (Scylla paramamosain) has received considerable attention, due to the remarkably biological and economic differences between sexes. However, sex-biased genes, especially non-coding genes, which account for these differences, remain elusive in this crustacean species. In this study, the first de novo gonad transcriptome sequencing was performed to identify both differentially expressed genes and long non-coding RNAs (lncRNAs) between male and female S...
November 20, 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/29151140/effects-of-oxygen-availability-on-oxidative-stress-biomarkers-in-the-mediterranean-mussel-mytilus-galloprovincialis
#12
Alessia Giannetto, Maria Maisano, Tiziana Cappello, Sabrina Oliva, Vincenzo Parrino, Antonino Natalotto, Giuseppe De Marco, Salvatore Fasulo
In aquatic environments, hypoxia and oxygen-deficient areas are increasing worldwide. Transitions in oxygen levels can influence the production of reactive oxygen species (ROS), eventually leading to oxidative stress. The transcriptional response of oxidative stress biomarkers was evaluated by qPCR in gill tissue from Mytilus galloprovincialis experimentally subjected to 48-h air exposure followed by 48-h re-oxygenation, as compared to normoxic control mussels. Superoxide dismutases (CuZnsod and Mnsod), catalase (cat), and glutathione S-transferase (gst) were over-expressed early after 8-h air exposure and returned to normoxic levels during re-oxygenation...
November 18, 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/29151139/integration-of-bacterial-expansin-on-agarolytic-complexes-to-enhance-the-degrading-activity-of-red-algae-by-control-of-gelling-properties
#13
Da Woon Jeong, Jeong Eun Hyeon, Young-Chul Joo, Sang Kyu Shin, Sung Ok Han
Expansin act by loosening hydrogen bonds in densely packed polysaccharides. This work characterizes the biological functions of expansin in the gelling and degradation of algal polysaccharides. In this study, the bacterial expansin BpEX from Bacillus pumilus was fused with the dockerin module of a cellulosome system for assembly with agarolytic complexes. The assembly of chimeric expansin caused an indicative enhancement in agarase activity. The enzymatic activities on agar substrate and natural biomass were 3...
November 18, 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/29127523/identification-of-quantitative-trait-loci-for-resistance-to-rsivd-in-red-sea-bream-pagrus-major
#14
Eitaro Sawayama, Shiho Tanizawa, Shin-Ichi Kitamura, Kei Nakayama, Kohei Ohta, Akiyuki Ozaki, Motohiro Takagi
Red sea bream iridoviral disease (RSIVD) is a major viral disease in red sea bream farming in Japan. Previously, we identified one candidate male individual of red sea bream that was significantly associated with convalescent individuals after RSIVD. The purpose of this study is to identify the quantitative trait loci (QTL) linked to the RSIVD-resistant trait for future marker-assisted selection (MAS). Two test families were developed using the candidate male in 2014 (Fam-2014) and 2015 (Fam-2015). These test families were challenged with RSIV, and phenotypes were evaluated...
November 10, 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/29080933/statistical-optimization-of-culture-variables-for-enhancing-agarase-production-by-dendryphiella-arenaria-utilizing-palisada-perforata-rhodophyta-and-enzymatic-saccharification-of-the-macroalgal-biomass
#15
Mohamed Gomaa, Awatief F Hifney, Mustafa A Fawzy, Khayria M Abdel-Gawad
Agarase is a promising biocatalyst for several industrial applications. Agarase production was evaluated by the marine fungus Dendryphiella arenaria utilizing Palisada perforata as a basal substrate in semi-solid state fermentation. Seaweed biomass, glucose, and sucrose were the most significant parameters affecting agarase production, and their levels were further optimized using Box-Behnken design. The maximum agarase activity was 7.69 U/mL. Agarase showed a degree of thermostability with half-life of 99 min at 40 °C, and declining to 44...
October 28, 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28980193/expression-of-thiaminase-in-zebrafish-danio-rerio-is-lethal-and-has-implications-for-use-as-a-biocontainment-strategy-in-aquaculture-and-invasive-species
#16
Sandra Noble, Vishal Saxena, Marc Ekker, Robert Devlin
As the world increasingly relies on aquaculture operations to meet rising seafood demands, reliable biocontainment measures for farmed fish stocks are desired to minimize ecological impacts arising from interactions of cultured fish with wild populations. One possible biocontainment strategy is to induce a dietary dependence on a vitamin, such as thiamine (vitamin B1), required for survival. Fish expressing thiaminase (an enzyme that degrades thiamine) within a confined aquaculture facility could receive supplemental thiamine to allow survival and normal growth, whereas escapees lacking this dietary rescue would die from thiamine deficiency...
October 5, 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28971324/a-genome-wide-association-study-reveals-that-genes-with-functions-for-bone-development-are-associated-with-body-conformation-in-catfish
#17
Xin Geng, Shikai Liu, Zihao Yuan, Yanliang Jiang, Degui Zhi, Zhanjiang Liu
Body conformation is of great scientific and commercial interest for aquaculture fish species because it affects biological adaptation of the organism to environments, and is of economic importance to the aquaculture industry considering its direct effect on fillet yield. Catfish is the primary aquaculture species in the USA. Two major species used in the aquaculture industry, channel catfish and blue catfish, differ in body shape and therefore the backcross progenies serve as a good model for quantitative trait locus (QTL) analysis...
October 2, 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28884399/bias-and-correction-in-rna-seq-data-for-marine-species
#18
Kai Song, Li Li, Guofan Zhang
RNA-seq is a recently developed approach widely used for transcriptome profiling in biological analyses that use next-generation sequencing technologies. Accurate estimation of gene expression levels is critical for answering biological questions. Here, we show that the commonly used measure of gene expression levels, fragments per kilobase of transcript per million mapped reads (FPKM), is biased in transcript length, GC content, and dinucleotide frequencies in the RNA-seq analysis of marine species. We used a generalized linear model to correct the observed biases of FPKM...
October 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28861717/estimates-of-heritability-for-growth-and-shell-color-traits-and-their-genetic-correlations-in-the-black-shell-strain-of-pacific-oyster-crassostrea-gigas
#19
Lan Xu, Qi Li, Hong Yu, Lingfeng Kong
The Pacific oyster Crassostrea gigas has been introduced widely and massively and became an economically important aquaculture species on a global scale. We estimated heritabilities of growth and shell color traits and their genetic correlations in black shell strain of C. gigas. Analyses were performed on 22 full-sib families in a nested mating design including 410 individuals at harvest (24 months of age). The parentage assignment was inferred based on four panels of multiplex PCR markers including 10 microsatellite loci and 94...
October 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28822037/effect-of-initiation-time-of-hydrostatic-pressure-shock-on-chromosome-set-doubling-of-tetraploidization-in-turbot-scophthalmus-maximus
#20
Xiangping Zhu, Zhengmei Lin, Zhihao Wu, Jiandong Li, Feng You
The objective of the study was to clarify the effects of initiation time on chromosome set doubling induced by hydrostatic pressure shock through nuclear phase fluorescent microscopy in turbot Scophthalmus maximus. The ratio of developmentally delayed embryo and chromosome counting was used to assess induction efficiency. For the embryos subjected to a pressure of 67.5 MPa for 6 min at prometaphase (A group), chromosomes recovered to the pre-treatment condition after 11-min recovering. The first nuclear division and cytokinesis proceeded normally...
October 2017: Marine Biotechnology
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