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Marine Biotechnology

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https://www.readbyqxmd.com/read/28527016/chemical-derivatization-of-metabolite-mass-profiling-of-the-recretohalophyte-aeluropus-lagopoides-revealing-salt-stress-tolerance-mechanism
#1
Murali Krishna Paidi, Parinita Agarwal, Prashant More, Pradeep K Agarwal
Plants are the primary producers of food for human being. Their intracellular environment alternation is influenced by abiotic stress factors such as drought, heat and soil salinity. Aeluropus lagopoides is a strong halophyte that grows with ease under high saline muddy banks of creeks of Gujarat, India. To study the response of salinity on metabolite changes in Aeluropus, three treatments, i.e. control, salinity and recovery, were selected for both shoot and root tissue. The cytosolic metabolite state was analysed by molecular chemical derivatization gas chromatography mass profiling...
May 19, 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28527015/a-genome-wide-association-study-identifies-the-genomic-region-associated-with-shell-color-in-yesso-scallop-patinopecten-yessoensis
#2
Liang Zhao, Yangping Li, Yajuan Li, Jiachen Yu, Huan Liao, Shuyue Wang, Jia Lv, Jun Liang, Xiaoting Huang, Zhenmin Bao
The shell color polymorphism widely exists in economic shellfish, which not only results in a better visual perception but also shows great value as an economic trait for breeding. Small numbers of reddish-orange shell Yesso scallops, Patinopecten yessoensis, were found in cultured populations compared to the brown majority. In this study, a genome-wide association study was conducted to understand the genetic basis of shell color. Sixty-six 2b-RAD libraries with equal numbers of reddish-orange and brown shell individuals were constructed and sequenced using the Illumina HiSeq 2000 platform...
May 19, 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28508952/identification-of-sex-determining-loci-in-pacific-white-shrimp-litopeneaus-vannamei-using-linkage-and-association-analysis
#3
Yang Yu, Xiaojun Zhang, Jianbo Yuan, Quanchao Wang, Shihao Li, Hao Huang, Fuhua Li, Jianhai Xiang
The Pacific white shrimp Litopenaeus vannamei is a predominant aquaculture shrimp species in the world. Like other animals, the L. vannamei exhibited sexual dimorphism in growth trait. Mapping of the sex-determining locus will be very helpful to clarify the sex determination system and further benefit the shrimp aquaculture industry towards the production of mono-sex stocks. Based on the data used for high-density linkage map construction, linkage-mapping analysis was conducted. The sex determination region was mapped in linkage group (LG) 18...
May 16, 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28501901/production-of-mycosporine-like-amino-acids-from-gracilaria-vermiculophylla-rhodophyta-cultured-through-one-year-in-an-integrated-multi-trophic-aquaculture-imta-system
#4
Marta Barceló-Villalobos, Félix L Figueroa, Nathalie Korbee, Félix Álvarez-Gómez, Maria H Abreu
This study evaluates the production of biomass and mycosporine-like amino acids (MAAs) throughout the year in Gracilaria vermiculophylla (Rhodophyta) collected in Ria de Aveiro (Portugal). The algae were grown in outdoor tanks in seawater with the addition of fishpond effluents under two different water flows (100 and 200 L h(-1)) in an integrated multi-trophic aquaculture (IMTA) system (tanks 1200 L; 1.5 m(2)) and different algal densities (3, 5, and 7 kg m(-2)). MAA content in IMTA seaweeds was significantly affected by the interaction of time and stocking density, but not by the water flow...
May 13, 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28500614/irf9-stat2-fusion-protein-as-an-innate-immune-inducer-to-activate-mx-and-interferon-stimulated-gene-expression-in-zebrafish-larvae
#5
Chang-Jen Huang, Chih-Ming Chou, Huang-Wei Lien, Cheng-Ying Chu, Jhih-Yun Ho, Yimin Wu, Chia-Hsiung Cheng
Virus infection often causes large amounts of mortality during teleost larvae stage. Strong induction of innate immunity to increase survival rates of teleost larvae has been less reported. In this study, we present a zebrafish IRF9-Stat2 fusion protein (zIRF9-S2C) as a strong innate immunity inducer and characterized induction of interferon-stimulated genes (ISGs) in zebrafish larvae. zIRF9-S2C could mimic IFN-stimulated gene factor 3 (ISGF3) complex to constitutively activate transcription of Mx promoter through IFN-stimulatory element (ISRE) sites...
May 12, 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28500613/functional-diets-modulate-lncrna-coding-rnas-and-gene-interactions-in-the-intestine-of-rainbow-trout-oncorhynchus-mykiss
#6
Gustavo Núñez-Acuña, Camille Détrée, Cristian Gallardo-Escárate, Ana Teresa Gonçalves
The advent of functional genomics has sparked the interest in inferring the function of non-coding regions from the transcriptome in non-model species. However, numerous biological processes remain understudied from this perspective, including intestinal immunity in farmed fish. The aim of this study was to infer long non-coding RNA (lncRNAs) expression profiles in rainbow trout (Oncorhynchus mykiss) fed for 30 days with functional diets based on pre- and probiotics. For this, whole transcriptome sequencing was conducted through Illumina technology, and lncRNAs were mined to evaluate transcriptional activity in conjunction with known protein sequences...
May 12, 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28493049/identification-and-differential-expression-of-biomineralization-genes-in-the-mantle-of-pearl-oyster-pinctada-fucata
#7
Haimei Li, Bo Zhang, Sigang Fan, Baosuo Liu, Jiaqi Su, Dahui Yu
A series of proteins are involved in shell formation of the pearl oyster Pinctada fucata, but the involved mechanisms and the relative expression levels of these proteins have not been elucidated. In this study, we sequenced and characterized the transcriptome of P. fucata mantle tissue. A total of 100,679 unique transcripts were assembled, 43687 Unigenes were annotated, and 48654 CDSs were determined. Of these, GO annotated 16353 Unigenes, COG defined 11585 unigenes into 25 categories, and KEGG sorted 25053 unigenes into 258 pathways...
May 10, 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28493048/a-novel-%C3%AF-3-desaturase-in-the-deep-sea-giant-tubeworm-riftia-pachyptila
#8
Helu Liu, Hui Wang, Shanya Cai, Haibin Zhang
One paradox of the trophic biochemistry of the deep sea giant tubeworm Riftia pachyptila, endemic to hydrothermal vent sites and nourished by polyunsaturated fatty acid (PUFA) deficiency chemolitoautotrophic sulfide-oxidizing bacteria, is the source of their PUFAs. Biosynthesis of PUFA starts with two precursors C18:2n-6 and C18:3n-3, which cannot be biosynthesized by most animals due to lack of ω6- and ω3-desaturase; thus, C18:2n-6 and C18:3n-3 are generally essential fatty acids for animals. Here, we characterized a gene derived from the R...
May 10, 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28484864/genome-wide-association-study-identifies-loci-associated-with-resistance-to-viral-nervous-necrosis-disease-in-asian-seabass
#9
Le Wang, Peng Liu, Shuqing Huang, Baoqing Ye, Elaine Chua, Zi Yi Wan, Gen Hua Yue
Viral nervous necrosis disease (VNN), caused by nervous necrosis virus (NNV), is one major threat to mariculture. Identifying loci and understanding the mechanisms associated with resistance to VNN are important in selective breeding programs. We performed a genome-wide association study (GWAS) using genotyping-by-sequencing (GBS) to study the genomic architecture of resistance to NNV infection in Asian seabass. We genotyped 986 individuals from 43 families produced by 15 founders with 44498 bi-allelic genetic variants using GBS...
May 8, 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28456869/fatty-acid-characterization-and-biodiesel-production-by-the-marine-microalga-asteromonas-gracilis-statistical-optimization-of-medium-for-biomass-and-lipid-enhancement
#10
Mustafa A Fawzy
Lipid production is an important indicator for evaluating microalgal species for biodiesel production. In this study, a new green microalga was isolated from a salt lake in Egypt and identified as Asteromonas gracilis. The main parameters such as biomass productivity, lipid content, and lipid productivity were evaluated in A. gracilis, cultivated in nutrient-starved (nitrogen, phosphorous), and salinity stress as a one-factor-at-a-time method. These parameters in general did not vary significantly from the standard nutrient growth media when these factors were utilized separately...
April 29, 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28421378/transcriptome-based-identification-of-the-desiccation-response-genes-in-marine-red-algae-pyropia-tenera-rhodophyta-and-enhancement-of-abiotic-stress-tolerance-by-ptdrg2-in-chlamydomonas
#11
Sungoh Im, Ha-Nul Lee, Hyun Shin Jung, Sunghwan Yang, Eun-Jeong Park, Mi Sook Hwang, Won-Joong Jeong, Dong-Woog Choi
Pyropia tenera (Kjellman) are marine red algae that grow in the intertidal zone and lose more than 90% of water during hibernal low tides every day. In order to identify the desiccation response gene (DRG) in P. tenera, we generated 1,444,210 transcriptome sequences using the 454-FLX platform from the gametophyte under control and desiccation conditions. De novo assembly of the transcriptome reads generated 13,170 contigs, covering about 12 Mbp. We selected 1160 differentially expressed genes (DEGs) in response to desiccation stress based on reads per kilobase per million reads (RPKM) expression values...
April 18, 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28378103/on-site-direct-detection-of-astaxanthin-from-salmon-fillet-using-raman-spectroscopy
#12
Jun-Ichi Hikima, Masahiro Ando, Hiro-O Hamaguchi, Masahiro Sakai, Masashi Maita, Kazunaga Yazawa, Haruko Takeyama, Takashi Aoki
A new technology employing Raman spectroscopy is attracting attention as a powerful biochemical technique for the detection of beneficial and functional food nutrients, such as carotenoids and unsaturated fatty acids. This technique allows for the dynamic characterization of food nutrient substances for the rapid determination of food quality. In this study, we attempt to detect and measure astaxanthin from salmon fillets using this technology. The Raman spectra showed specific bands corresponding to the astaxanthin present in salmon and the value of astaxanthin (Raman band, 1518 cm(-1)) relative to those of protein/lipid (Raman band, 1446 cm(-1)) in the spectra increased in a dose-dependent manner...
April 4, 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28280946/efficacy-and-ecotoxicity-of-novel-anti-fouling-nanomaterials-in-target-and-non-target-marine-species
#13
Francisco Avelelas, Roberto Martins, Tânia Oliveira, Frederico Maia, Eliana Malheiro, Amadeu M V M Soares, Susana Loureiro, João Tedim
Biofouling is a global problem that affects virtually all the immersed structures. Currently, several novel environmentally friendly approaches are being tested worldwide to decrease the toxicity of biocides in non-fouling species, such as the encapsulation/immobilization of commercially available biocides, in order to achieve control over the leaching rate. The present study addresses the toxicity of two widely used booster biocides, zinc pyrithione (ZnPT) and copper pyrithione (CuPT), in its free and incorporated forms in order to assess their toxicity and anti-fouling efficacy in target and non-target species...
March 9, 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28246982/transcriptomic-analysis-of-male-black-tiger-shrimp-penaeus-monodon-after-polychaete-feeding-to-enhance-testicular-maturation
#14
Rungnapa Leelatanawit, Umaporn Uawisetwathana, Amornpan Klanchui, Jutatip Khudet, Suwanchai Phomklad, Somjai Wongtriphop, Pikul Jiravanichpaisal, Nitsara Karoonuthaisiri
To reveal molecular mechanism of how polychaetes enhanced reproductive maturation in the male black tiger shrimp (Penaeus monodon), transcriptomic profiles of male reproductive organs (testes and vas deferens) between polychaete-fed and commercial pellet-fed male brooders were compared using cDNA microarray. The overall profiles were distinguishingly different between the two feed groups as well as between testes and vas deferens. Additionally, six of 11 differentially expressed gene identified by the microarray (HNRPUL1 and GCP4 in testes, MAT2B, CDC16, and CSN5 in vas deferens, and SLD5 in both organs) were validated by quantitative real-time PCR (qPCR) and found to exhibit significantly higher expression levels in polychaete-fed shrimp than those in commercial pellet-fed shrimp...
February 28, 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28349286/epipodial-tentacle-gene-expression-and-predetermined-resilience-to-summer-mortality-in-the-commercially-important-greenlip-abalone-haliotis-laevigata
#15
Brett P Shiel, Nathan E Hall, Ira R Cooke, Nicholas A Robinson, Jan M Strugnell
"Summer mortality" is a phenomenon that occurs during warm water temperature spikes that results in the mass mortality of many ecologically and economically important mollusks such as abalone. This study aimed to determine whether the baseline gene expression of abalone before a laboratory-induced summer mortality event was associated with resilience to summer mortality. Tentacle transcriptomes of 35 greenlip abalone (Haliotis laevigata) were sequenced prior to the animals being exposed to an increase in water temperature-simulating conditions which have previously resulted in summer mortality...
April 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28283802/nitrogen-metabolism-genes-from-temperate-marine-sediments
#16
Carolina Reyes, Dominik Schneider, Marko Lipka, Andrea Thürmer, Michael E Böttcher, Michael W Friedrich
In this study, we analysed metagenomes along with biogeochemical profiles from Skagerrak (SK) and Bothnian Bay (BB) sediments, to trace the prevailing nitrogen pathways. NO3(-) was present in the top 5 cm below the sediment-water interface at both sites. NH4(+) increased with depth below 5 cm where it overlapped with the NO3(-) zone. Steady-state modelling of NO3(-) and NH4(+) porewater profiles indicates zones of net nitrogen species transformations. Bacterial protease and hydratase genes appeared to make up the bulk of total ammonification genes...
April 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28233074/construction-of-plastid-expression-vector-and-development-of-genetic-transformation-system-for-the-seaweed-pyropia-yezoensis
#17
Fanna Kong, Hailong Zhao, Weixun Liu, Na Li, Yunxiang Mao
Pyropia yezoensis, belonging to the Rhodophyta, is an economically important seaweed. In this study, we developed a high-efficiency plastid transformation platform for P. yezoensis. In the plastid transformation vector, psbA UTR of P. yezoensis, including the promoter and 3' UTR, was used to express foreign genes. The integration site was a transcriptionally active intergenic region between the rrsB and trnI genes, located in the inverted repeat regions of the plastid genome. The CAT and eGFP genes were integrated into the plastid genome at this site...
February 23, 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28161850/potential-anti-proliferative-and-immunomodulatory-effects-of-marine-microalgal-exopolysaccharide-on-various-human-cancer-cells-and-lymphocytes-in-vitro
#18
Geon-Tae Park, Ryeo-Eun Go, Hae-Miru Lee, Geum-A Lee, Cho-Won Kim, Jeong-Woo Seo, Won-Kyung Hong, Kyung-Chul Choi, Kyung-A Hwang
Marine microalgal exopolysaccharides (EPSs) have drawn great attention due to their biotechnological potentials such as anti-viral, anti-oxidant, anti-lipidemic, anti-proliferative, and immunomodulatory activities, etc. In the present study, the EPS derived from microalgae Thraustochytriidae sp.-derived mutant GA was investigated for its anti-proliferation and immunomodulation. Anti-cancer efficacy of the microalgal EPS was examined for the alterations in cell proliferation and cell cycle-related gene expression that occur in three types of human cancer cell lines, BG-1 ovarian, MCF-7 breast, and SW-620 colon cancer cell lines, by its treatment...
February 4, 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28204970/a-carbonic-anhydrase-serves-as-an-important-acid-base-regulator-in-pacific-oyster-crassostrea-gigas-exposed-to-elevated-co2-implication-for-physiological-responses-of-mollusk-to-ocean-acidification
#19
Xiudan Wang, Mengqiang Wang, Zhihao Jia, Limei Qiu, Lingling Wang, Anguo Zhang, Linsheng Song
Carbonic anhydrases (CAs) have been demonstrated to play an important role in acid-base regulation in vertebrates. However, the classification and modulatory function of CAs in marine invertebrates, especially their responses to ocean acidification remain largely unknown. Here, a cytosolic α-CA (designated as CgCAII-1) was characterized from Pacific oyster Crassostrea gigas and its molecular activities against CO2 exposure were investigated. CgCAII-1 possessed a conserved CA catalytic domain, with high similarity to invertebrate cytoplasmic or mitochondrial α-CAs...
February 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28204969/convergent-evolution-of-the-osmoregulation-system-in-decapod-shrimps
#20
Jianbo Yuan, Xiaojun Zhang, Chengzhang Liu, Hu Duan, Fuhua Li, Jianhai Xiang
In adaptating to different aquatic environments, seawater (SW) and freshwater (FW) shrimps have exploited different adaptation strategies, which should generate clusters of genes with different adaptive features. However, little is known about the genetic basis of these physiological adaptations. Thus, in this study, we performed comparative transcriptomics and adaptive evolution analyses on SW and FW shrimps and found that convergent evolution may have happened on osmoregulation system of shrimps. We identified 275 and 234 positively selected genes in SW and FW shrimps, respectively, which enriched in the functions of ion-binding and membrane-bounded organelles...
February 2017: Marine Biotechnology
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