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Marine Biotechnology

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https://www.readbyqxmd.com/read/28283802/nitrogen-metabolism-genes-from-temperate-marine-sediments
#1
Carolina Reyes, Dominik Schneider, Marko Lipka, Andrea Thürmer, Michael E Böttcher, Michael W Friedrich
In this study, we analysed metagenomes along with biogeochemical profiles from Skagerrak (SK) and Bothnian Bay (BB) sediments, to trace the prevailing nitrogen pathways. NO3(-) was present in the top 5 cm below the sediment-water interface at both sites. NH4(+) increased with depth below 5 cm where it overlapped with the NO3(-) zone. Steady-state modelling of NO3(-) and NH4(+) porewater profiles indicates zones of net nitrogen species transformations. Bacterial protease and hydratase genes appeared to make up the bulk of total ammonification genes...
March 10, 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28280946/efficacy-and-ecotoxicity-of-novel-anti-fouling-nanomaterials-in-target-and-non-target-marine-species
#2
Francisco Avelelas, Roberto Martins, Tânia Oliveira, Frederico Maia, Eliana Malheiro, Amadeu M V M Soares, Susana Loureiro, João Tedim
Biofouling is a global problem that affects virtually all the immersed structures. Currently, several novel environmentally friendly approaches are being tested worldwide to decrease the toxicity of biocides in non-fouling species, such as the encapsulation/immobilization of commercially available biocides, in order to achieve control over the leaching rate. The present study addresses the toxicity of two widely used booster biocides, zinc pyrithione (ZnPT) and copper pyrithione (CuPT), in its free and incorporated forms in order to assess their toxicity and anti-fouling efficacy in target and non-target species...
March 9, 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28246982/transcriptomic-analysis-of-male-black-tiger-shrimp-penaeus-monodon-after-polychaete-feeding-to-enhance-testicular-maturation
#3
Rungnapa Leelatanawit, Umaporn Uawisetwathana, Amornpan Klanchui, Jutatip Khudet, Suwanchai Phomklad, Somjai Wongtriphop, Pikul Jiravanichpaisal, Nitsara Karoonuthaisiri
To reveal molecular mechanism of how polychaetes enhanced reproductive maturation in the male black tiger shrimp (Penaeus monodon), transcriptomic profiles of male reproductive organs (testes and vas deferens) between polychaete-fed and commercial pellet-fed male brooders were compared using cDNA microarray. The overall profiles were distinguishingly different between the two feed groups as well as between testes and vas deferens. Additionally, six of 11 differentially expressed gene identified by the microarray (HNRPUL1 and GCP4 in testes, MAT2B, CDC16, and CSN5 in vas deferens, and SLD5 in both organs) were validated by quantitative real-time PCR (qPCR) and found to exhibit significantly higher expression levels in polychaete-fed shrimp than those in commercial pellet-fed shrimp...
February 28, 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28233074/construction-of-plastid-expression-vector-and-development-of-genetic-transformation-system-for-the-seaweed-pyropia-yezoensis
#4
Fanna Kong, Hailong Zhao, Weixun Liu, Na Li, Yunxiang Mao
Pyropia yezoensis, belonging to the Rhodophyta, is an economically important seaweed. In this study, we developed a high-efficiency plastid transformation platform for P. yezoensis. In the plastid transformation vector, psbA UTR of P. yezoensis, including the promoter and 3' UTR, was used to express foreign genes. The integration site was a transcriptionally active intergenic region between the rrsB and trnI genes, located in the inverted repeat regions of the plastid genome. The CAT and eGFP genes were integrated into the plastid genome at this site...
February 23, 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28161850/potential-anti-proliferative-and-immunomodulatory-effects-of-marine-microalgal-exopolysaccharide-on-various-human-cancer-cells-and-lymphocytes-in-vitro
#5
Geon-Tae Park, Ryeo-Eun Go, Hae-Miru Lee, Geum-A Lee, Cho-Won Kim, Jeong-Woo Seo, Won-Kyung Hong, Kyung-Chul Choi, Kyung-A Hwang
Marine microalgal exopolysaccharides (EPSs) have drawn great attention due to their biotechnological potentials such as anti-viral, anti-oxidant, anti-lipidemic, anti-proliferative, and immunomodulatory activities, etc. In the present study, the EPS derived from microalgae Thraustochytriidae sp.-derived mutant GA was investigated for its anti-proliferation and immunomodulation. Anti-cancer efficacy of the microalgal EPS was examined for the alterations in cell proliferation and cell cycle-related gene expression that occur in three types of human cancer cell lines, BG-1 ovarian, MCF-7 breast, and SW-620 colon cancer cell lines, by its treatment...
February 4, 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28204970/a-carbonic-anhydrase-serves-as-an-important-acid-base-regulator-in-pacific-oyster-crassostrea-gigas-exposed-to-elevated-co2-implication-for-physiological-responses-of-mollusk-to-ocean-acidification
#6
Xiudan Wang, Mengqiang Wang, Zhihao Jia, Limei Qiu, Lingling Wang, Anguo Zhang, Linsheng Song
Carbonic anhydrases (CAs) have been demonstrated to play an important role in acid-base regulation in vertebrates. However, the classification and modulatory function of CAs in marine invertebrates, especially their responses to ocean acidification remain largely unknown. Here, a cytosolic α-CA (designated as CgCAII-1) was characterized from Pacific oyster Crassostrea gigas and its molecular activities against CO2 exposure were investigated. CgCAII-1 possessed a conserved CA catalytic domain, with high similarity to invertebrate cytoplasmic or mitochondrial α-CAs...
February 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28204969/convergent-evolution-of-the-osmoregulation-system-in-decapod-shrimps
#7
Jianbo Yuan, Xiaojun Zhang, Chengzhang Liu, Hu Duan, Fuhua Li, Jianhai Xiang
In adaptating to different aquatic environments, seawater (SW) and freshwater (FW) shrimps have exploited different adaptation strategies, which should generate clusters of genes with different adaptive features. However, little is known about the genetic basis of these physiological adaptations. Thus, in this study, we performed comparative transcriptomics and adaptive evolution analyses on SW and FW shrimps and found that convergent evolution may have happened on osmoregulation system of shrimps. We identified 275 and 234 positively selected genes in SW and FW shrimps, respectively, which enriched in the functions of ion-binding and membrane-bounded organelles...
February 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28181038/transcriptomic-profiling-of-egg-quality-in-sea-bass-dicentrarchus-labrax-sheds-light-on-genes-involved-in-ubiquitination-and-translation
#8
Daniel Żarski, Thaovi Nguyen, Aurélie Le Cam, Jérôme Montfort, Gilbert Dutto, Marie Odile Vidal, Christian Fauvel, Julien Bobe
Variable and low egg quality is a major limiting factor for the development of efficient aquaculture production. This stems from limited knowledge on the mechanisms underlying egg quality in cultured fish. Molecular analyses, such as transcriptomic studies, are valuable tools to identify the most important processes modulating egg quality. However, very few studies have been devoted to this aspect so far. Within this study, the microarray-based transcriptomic analysis of eggs (of different quality) of sea bass (Dicentrarchus labrax) was performed...
February 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28181037/de-novo-transcriptome-characterization-and-growth-related-gene-expression-profiling-of-diploid-and-triploid-bighead-catfish-clarias-macrocephalus-g%C3%A3-nther-1864
#9
Satid Chatchaiphan, Prapansak Srisapoome, Jin-Hyoung Kim, Robert H Devlin, Uthairat Na-Nakorn
To enhance understanding of triploid gene expression, the transcriptome information from bighead catfish (Clarias macrocephalus Günther, 1864) was studied using the paired-end Illumina HiSeq™ 2000 sequencing platform. In total, 68,227,832 raw reads were generated from liver tissues and 53,149 unigenes were assembled, with an average length of 765 bp and N50 length of 1283 bp. Of these unigenes, 33,428 (62.89%) could be annotated according to their homology with matches in the NCBI non-redundant (Nr), NCBI nucleotide (Nt), Swiss-Prot, Clusters of Orthologous Groups (COG), gene ontology (GO), or Kyoto Encyclopedia of Genes and Genomes (KEGG) databases...
February 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28168542/copy-number-variations-in-tilapia-genomes
#10
Bi Jun Li, Hong Lian Li, Zining Meng, Yong Zhang, Haoran Lin, Gen Hua Yue, Jun Hong Xia
Discovering the nature and pattern of genome variation is fundamental in understanding phenotypic diversity among populations. Although several millions of single nucleotide polymorphisms (SNPs) have been discovered in tilapia, the genome-wide characterization of larger structural variants, such as copy number variation (CNV) regions has not been carried out yet. We conducted a genome-wide scan for CNVs in 47 individuals from three tilapia populations. Based on 254 Gb of high-quality paired-end sequencing reads, we identified 4642 distinct high-confidence CNVs...
February 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28161851/genome-sequence-of-microbulbifer-mangrovi-dd-13-t-reveals-its-versatility-to-degrade-multiple-polysaccharides
#11
Md Imran, Poonam Pant, Yogini P Shanbhag, Samir V Sawant, Sanjeev C Ghadi
Microbulbifer mangrovi strain DD-13(T) is a novel-type species isolated from the mangroves of Goa, India. The draft genome sequence of strain DD-13 comprised 4,528,106 bp with G+C content of 57.15%. Out of 3479 open reading frames, functions for 3488 protein coding sequences were predicted on the basis of similarity with the cluster of orthologous groups. In addition to protein coding sequences, 34 tRNA genes and 3 rRNA genes were detected. Analysis of nucleotide sequence of predicted gene using a Carbohydrate-Active Enzymes (CAZymes) Analysis Toolkit indicates that strain DD-13 encodes a large set of CAZymes including 255 glycoside hydrolases, 76 carbohydrate esterases, 17 polysaccharide lyases, and 113 carbohydrate-binding modules (CBMs)...
February 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28150103/purification-biochemical-characterization-and-amino-acid-sequence-of-a-novel-type-of-lectin-from-aplysia-dactylomela-eggs-with-antibacterial-antibiofilm-potential
#12
Rômulo Farias Carneiro, Renato Cézar Farias Torres, Renata Pinheiro Chaves, Mayron Alves de Vasconcelos, Bruno Lopes de Sousa, André Castelo Rodrigues Goveia, Francisco Vassiliepe Arruda, Maria Nágila Carneiro Matos, Helena Matthews-Cascon, Valder Nogueira Freire, Edson Holanda Teixeira, Celso Shiniti Nagano, Alexandre Holanda Sampaio
A new lectin from Aplysia dactylomela eggs (ADEL) was isolated by affinity chromatography on HCl-activated Sepharose™ media. Hemagglutination caused by ADEL was inhibited by several galactosides, mainly galacturonic acid (Ka = 6.05 × 10(6) M(-1)). The primary structure of ADEL consists of 217 residues, including 11 half-cystines involved in five intrachain and one interchain disulfide bond, resulting in a molecular mass of 57,228 ± 2 Da, as determined by matrix-assisted laser desorption/ionization time of flight mass spectrometry...
February 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28138936/rna-seq-analysis-of-the-antioxidant-status-and-immune-response-of-portunus-trituberculatus-following-aerial-exposure
#13
Yuquan Li, Shoumin Lai, Renjie Wang, Yuchao Zhao, Hao Qin, Lingxu Jiang, Na Li, Qiang Fu, Chao Li
Desiccation tolerance has been long considered as an important trait for the life survival under acute environmental stress. One of the biggest problems for modern commercial crab farming is desiccation during transportation; high mortality could occur following the aerial exposure. In this regard, here, we utilized RNA-seq-based transcriptome profiling to characterize the molecular responses of swimming crab in response to aerial exposure. In present study, following aerial exposure, the gill samples were sequenced at 0, 6, 12, and 18 h...
February 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28138935/immunostimulatory-effect-of-enzyme-modified-hizikia-fusiformein-a-mouse-model-in-vitro-and-ex-vivo
#14
Sang-Yong Park, Eunson Hwang, Yu-Kyong Shin, Don-Gil Lee, Jung-Eun Yang, Jae-Hee Park, Tae-Hoo Yi
Hizikia fusiforme, a brown seaweed, has been utilized as a health food and in traditional medicine. In this study, we investigated whether enzyme-modified H. fusiforme extracts (EH) have immunological effects compared with normal H. fusiforme extracts (NH). The effects of NH and EH on immune responses were investigated by assessing nitric oxide (NO) production, phagocytosis, and cytokine secretion in RAW 264.7 murine macrophages and mice. Also, fucosterol was evaluated to find the active component of NH and EH by addressing cytotoxicity test and NO production...
February 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28083715/characterization-of-luxi-and-luxr-protein-homologs-of-n-acylhomoserine-lactone-dependent-quorum-sensing-system-in-pseudoalteromonas-sp-520p1
#15
Hoang Tran Dang, Shinya Komatsu, Hideyuki Masuda, Keiichi Enomoto
Pseudoalteromonas sp. 520P1 (hereafter referred to as strain 520P1) produces N-acylhomoserine lactones (AHLs), which serve as signaling molecules in Gram-negative bacterial quorum sensing. In a previous genomic analysis of the 5.25-Mb genome of strain 520P1, we detected the presence of at least one homolog of the AHL synthase gene (luxI) and five homologs of the transcriptional regulator protein gene (luxR). The LuxI homolog of strain 520P1 (PalI) contained the conserved amino acid motifs shared by all the LuxI family proteins of the different species examined here...
February 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/27909912/identification-and-characterization-of-the-lysine-rich-matrix-protein-family-in-pinctada-fucata-indicative-of-roles-in-shell-formation
#16
Jian Liang, Jun Xie, Jing Gao, Chao-Qun Xu, Yi Yan, Gan-Chu Jia, Liang Xiang, Li-Ping Xie, Rong-Qing Zhang
Mantle can secret matrix proteins playing key roles in regulating the process of shell formation. The genes encoding lysine-rich matrix proteins (KRMPs) are one of the most highly expressed matrix genes in pearl oysters. However, the expression pattern of KRMPs is limited and the functions of them still remain unknown. In this study, we isolated and identified six new members of lysine-rich matrix proteins, rich in lysine, glycine and tyrosine, and all of them are basic matrix proteins. Combined with four members of the KRMPs previously reported, all these proteins can be divided into three subclasses according to the results of phylogenetic analyses: KRMP1-3 belong to subclass KPI, KRMP4-5 belong to KPII, and KRMP6-10 belong to KPIII...
December 2, 2016: Marine Biotechnology
https://www.readbyqxmd.com/read/27888371/protective-effect-of-the-sulfated-agaran-isolated-from-the-red-seaweed-laurencia-aldingensis-against-toxic-effects-of-the-venom-of-the-snake-lachesis-muta
#17
Ana Cláudia Rodrigues da Silva, Luciana Garcia Ferreira, Maria Eugênia Rabello Duarte, Mutue Toyota Fujii, Eladio Flores Sanchez, Miguel Daniel Noseda, André Lopes Fuly
Snakebite is a serious occupational hazard affecting mainly rural populations of tropical and subtropical developing countries. Lachesis muta (Bushmaster) bites are extremely serious but are rarely reported in the literature. Bushmaster envenomings are characterized by intense local pain, edema, neurotoxicity, hypotension, local hemorrhage, and dramatic systemic alterations. Antivenom treatment has regularly been used for more than a century; however, it fails to neutralize local tissue damage and hemorrhage, leading to morbidity or disabilities in victims...
November 25, 2016: Marine Biotechnology
https://www.readbyqxmd.com/read/27844293/widespread-distribution-of-dehalococcoides-mccartyi-in-the-houston-ship-channel-and-galveston-bay-texas-sediments-and-the-potential-for-reductive-dechlorination-of-pcdd-f-in-an-estuarine-environment
#18
Anne-Sophie Charlotte Hieke, Robin Brinkmeyer, Kevin M Yeager, Kimberly Schindler, Saijin Zhang, Chen Xu, Patrick Louchouarn, Peter H Santschi
Sediments in the Houston Ship Channel and upper Galveston Bay, Texas, USA, are polluted with polychlorinated dibenzo-p-dioxins/furans (PCDD/F; ≤46,000 ng/kg dry weight (wt.)) with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), the most toxic congener, contributing >50 % of the total toxic equivalents (TEQ) at most locations. We measured PCDD/F concentrations in sediments and evaluated the potential for enhanced in situ biodegradation by surveying for Dehalococcoides mccartyi, an obligate organohalide respiring bacterium...
November 14, 2016: Marine Biotechnology
https://www.readbyqxmd.com/read/27819120/metagenomic-analysis-of-genes-encoding-nutrient-cycling-pathways-in-the-microbiota-of-deep-sea-and-shallow-water-sponges
#19
Zhiyong Li, Yuezhu Wang, Jinlong Li, Fang Liu, Liming He, Ying He, Shenyue Wang
Sponges host complex symbiotic communities, but to date, the whole picture of the metabolic potential of sponge microbiota remains unclear, particularly the difference between the shallow-water and deep-sea sponge holobionts. In this study, two completely different sponges, shallow-water sponge Theonella swinhoei from the South China Sea and deep-sea sponge Neamphius huxleyi from the Indian Ocean, were selected to compare their whole symbiotic communities and metabolic potential, particularly in element transformation...
November 7, 2016: Marine Biotechnology
https://www.readbyqxmd.com/read/27778119/identification-of-mitochondrial-genome-encoded-small-rnas-related-to-egg-deterioration-caused-by-postovulatory-aging-in-rainbow-trout
#20
Hao Ma, Gregory M Weber, Hairong Wei, Jianbo Yao
Many factors have been reported to affect rainbow trout egg quality, among which, postovulatory aging is one of the most significant causes as reared rainbow trout do not usually volitionally oviposit the ovulated eggs. In order to uncover the genetic regulation underling egg deterioration caused by postovulatory aging in rainbow trout, mitochondrial genome-encoded small RNA (mitosRNAs) were analyzed from unfertilized eggs on Days 1, 7, and 14 postovulation with fertilization rates of 91.8, 73.4, and less than 50 %, respectively...
October 2016: Marine Biotechnology
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