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Journal of Bioscience and Bioengineering

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https://www.readbyqxmd.com/read/28818427/molecular-cloning-and-functional-characterization-of-nadph-dependent-cytochrome-p450-reductase-from-the-green-microalga-botryococcus-braunii-b-race
#1
Chung-Yau Tsou, Shigeki Matsunaga, Shigeru Okada
The green microalga Botryococcus braunii of the B race accumulates various lipophilic compounds containing a 10,11-oxidosqualene epoxide moiety in addition to large amounts of triterpene hydrocarbons. While 2,3-squalene epoxidases have already been isolated and characterized from the alga, the enzyme that catalyzes the 10,11-epoxidation of squalene has remained elusive. In order to obtain a molecular tool to explore a 10,11-squalene epoxidase, cDNA cloning of an NADPH-dependent cytochrome P450 reductase (CPR) that is required by both squalene epoxidases and cytochrome P450 enzymes was carried out...
August 14, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28818426/investigation-of-the-escherichia-coli-membrane-transporters-involved-in-the-secretion-of-d-lactate-based-oligomers-by-loss-of-function-screening
#2
Camila Utsunomia, Chiaki Hori, Ken'ichiro Matsumoto, Seiichi Taguchi
d-Lactate (LA)-based oligomers (D-LAOs) are unusual oligoesters consisting of d-LA and d-3-hydroxybutyrate that are produced and secreted by engineered Escherichia coli grown on glucose. The cells heterologously express LA-polymerizing polyhydroxyalkanoate synthase and monomer-supplying enzymes. In this study, we attempted to identify the D-LAO secretion route in E. coli, which is thought to be mediated by intrinsic membrane proteins. To this end, a loss-of-function screening of D-LAO secretion was carried out using 209 single-gene membrane protein deletants, which are involved in the transport of organic compounds...
August 14, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28811097/type-i-collagen-promotes-primary-cilia-growth-through-down-regulating-hdac6-mediated-autophagy-in-confluent-mouse-embryo-fibroblast-3t3-l1-cells
#3
Qian Xu, Weiwei Liu, Xiaoling Liu, Wuxiyar Otkur, Toshihiko Hayashi, Masayuki Yamato, Hitomi Fujisaki, Shunji Hattori, Shin-Ichi Tashiro, Takashi Ikejima
Primary cilia are microtubule-based organelles that extend from nearly all vertebrate cells. Abnormal ciliogenesis and cilia length are suggested to be associated with hypertension and obesity as well as diseases such as Meckel-Gruber syndrome. Extracellular matrix (ECM), comprising cellular microenvironment, influences cell shape and proliferation. However, influence of ECM on cilia biogenesis has not been well studied. In this study we examined the effects of type I collagen (col I), the major component of ECM, on primary cilia growth...
August 12, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28803053/optimized-transitory-ectopic-expression-of-promastigote-surface-antigen-protein-in-nicotiana-benthamiana-a-potential-anti-leishmaniasis-vaccine-candidate
#4
Séverine Lacombe, Martine Bangratz, Jean-Paul Brizard, Elodie Petitdidier, Julie Pagniez, Drissa Sérémé, Jean-Loup Lemesre, Christophe Brugidou
In recent years, plants have been shown to be an efficient alternative expression system for high-value pharmaceuticals such as vaccines. However, constitutive expression of recombinant protein remains uncertain on their level of production and biological activity. To overcome these problems, transitory expression systems have been developed. Here, a series of experiments were performed to determine the most effective conditions to enhance vaccine antigen transient accumulation in Nicotiana benthamiana leaves using the promastigote surface antigen (PSA) from the parasitic protozoan Leishmania infantum...
August 10, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28803054/decolorization-of-reactive-azo-dyes-using-a-sequential-chemical-and-activated-sludge-treatment
#5
Ken Meerbergen, Sam Crauwels, Kris A Willems, Raf Dewil, Jan Van Impe, Lise Appels, Bart Lievens
Textile wastewater contains high concentrations of organic substances derived from diverse dyes and auxiliary chemicals, some of which are non-biodegradable and/or toxic. Therefore, it is essential that textile wastewater is treated and that these substances are removed before being discharged into the environment. A combination of advanced oxidation processes (AOPs) to obtain partial dye degradation followed by a biological treatment has been suggested as a promising method for cost-effective decolorization of wastewater...
August 9, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28800907/biochemical-characterization-of-aspergillus-oryzae-recombinant-%C3%AE-l-rhamnosidase-expressed-in-pichia-pastoris
#6
Mai Ishikawa, Yoshihito Shiono, Takuya Koseki
An α-l-rhamnosidase-encoding gene from Aspergillus oryzae, which belongs to the glycoside hydrolase family 78, was cloned and expressed in Pichia pastoris. SDS-PAGE of the purified recombinant α-l-rhamnosidase protein revealed smeared bands with apparent molecular mass of 90-130 kDa. After N-deglycosylation, the recombinant enzyme showed a molecular mass of 70 kDa. The enzyme exhibited optimal activity at a pH of 5.0 and a temperature of 70 °C. Specific activity of the enzyme was higher toward hesperidin than toward naringin, which consist of α-1,6 and α-1,2 linkages, respectively...
August 9, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28800908/stimulation-of-d-and-l-lactate-dehydrogenases-transcriptional-levels-in-presence%C3%A2-of-diammonium-hydrogen-phosphate-resulting-to-enhanced-lactic-acid%C3%A2-production-by-lactobacillus-strain
#7
Mamata Singhvi, Takeshi Zendo, Hiroshi Iida, Digambar Gokhale, Kenji Sonomoto
The present study revealed the effect of nitrogen sources on lactic acid production and stimulation of d- and l-lactate dehydrogenases (LDH) of parent Lactobacillus lactis NCIM 2368 and its mutant RM2-24 generated after UV mutagenesis. Both the parent and mutant strains were evaluated for d-lactic acid production in control and modified media. The modified media did not show remarkable effect on lactic acid production in case of parent whereas mutant exhibited significant enhancement in d-lactic acid production along with the appearance of l-lactic acid in the broth...
August 8, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28800906/solid-phase-analytical-derivatization-for-gas-chromatography-mass-spectrometry-based-metabolomics
#8
Emi Takeo, Ryoichi Sasano, Shuichi Shimma, Takeshi Bamba, Eiichiro Fukusaki
A novel derivatization method for gas chromatography/mass spectrometry (GC/MS)-based metabolomics was developed, based on solid-phase analytical derivatization (SPAD) with methoximation followed by trimethylsilylation. This SPAD method realized derivatization on solid phases combining strong anion exchange with strong cation exchange. To omit a sample condensation process, GC/MS injection was performed using a large-volume injection mode. This mode uses a stomach-shaped insert, and enables a large quantity of sample to be vaporized and introduced into the GC/MS system...
August 8, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28797634/ecological-distribution-of-extremely-thermophilic-bacteria-belonging-to-the-genus-calditerricola-using-the-novel-enrichment-mpn-pcr-method
#9
Kathrina Mae Bienes, Minoru Ito, Kota Shiotsuka, Sachi Yamaguchi, Taiki Fujioka, Yukihiro Tashiro, Kenji Sakai
A unique compost called Satsuma soil is produced from sewage sludge by a hyperthermal composting process in Kagoshima City, Japan. The composting process is carried out at a controlled temperature of at least 80°C and the resulting compost might be useful for recycling sustainable agricultural products. The extremely thermophilic bacterial genus Calditerricola was initially isolated from the high-temperature compost. Likewise, the bacteria were previously isolated from material sludge. It is believed that bacteria in this genus might be involved in the hyperthermal composting process...
August 7, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28751126/intracellular-ca-2-is-an-essential-factor-for-cell-damage-induced-by-unsaturated-carbonyl-compounds
#10
Tsunehito Higashi, Yosuke Mai, Yuichi Mazaki, Soichi Miwa
The unsaturated carbonyl compounds are known as the environmental pollutants. Acrolein (ACR) and methyl vinyl ketone (MVK) are representative unsaturated carbonyl compounds. ACR is contained in smoke, automobile exhaust, industrial waste, and several foods. MVK is widely used as the industrial chemical. Although ACR and MVK are highly toxic, the molecular mechanism for their cytotoxicity has been unclear. We have previously reported that ACR and MVK are major cytotoxic compounds in the gas phase of cigarette smoke, and protein kinase C (PKC) inhibitor and NADPH oxidases inhibitor partially rescued cells from ACR- or MVK-induced cell death (Noya et al...
July 24, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28743658/high-level-expression-and-characterization-of-solvent-tolerant-lipase
#11
Yu Wang, Dan Luo, Yunshi Zhao, Shufang Tian, Wenpeng Deng, Chunhua Li, Lixin Ma
In this study, the coding sequence of the lipase from Proteus sp. SW1 was optimized via codon optimization and subjected to expression in Pichia pastoris GS115. The maximum enzyme yield was 387 mg/L in the supernatants of the shake-flask culture. The purified recombinant lipase exhibited a specific activity of 130 U/mg toward p-nitrophenyl Laurate. Its optimum pH and temperature were 8.0 and 40°C, respectively. It was highly stable and even activated in water-miscible solvents, showing over 102% residual activity after 24 h incubation in ethanol, acetone, isopropanol and acetonitrile...
July 22, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28743657/vinegar-production-from-post-distillation-slurry-deriving-from-rice-shochu-production-with-the-addition-of-caproic-acid-producing-bacteria-consortium-and-lactic-acid-bacterium
#12
Hua-Wei Yuan, Li Tan, Hao Chen, Zhao-Yong Sun, Yue-Qin Tang, Kenji Kida
To establish a zero emission process, the post-distillation slurry of a new type of rice shochu (NTRS) was used for the production of health promoting vinegar. Since the NTRS post-distillation slurry contained caproic acid and lactic acid, the effect of these two organic acids on acetic acid fermentation was first evaluated. Based on these results, Acetobacter aceti CICC 21684 was selected as a suitable strain for subsequent production of vinegar. At the laboratory scale, acetic acid fermentation of the NTRS post-distillation slurry in batch mode resulted in an acetic acid concentration of 41...
July 22, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28743656/development-of-a-rapid-immunoassay-system-luminescent-detection-of-antigen-associated-antibody-luciferase-in-the-presence-of-a-dye-that-absorbs-light%C3%A2-from-free-antibody-luciferase
#13
Akihiro Mori, Teruyo Ojima-Kato, Shingo Fuchi, Shinichi Kaiya, Takaaki Kojima, Hideo Nakano
In this report, we developed a rapid immunoassay system, designated the bioluminescent interference gathering optical (BINGO) assay, which required no time-consuming washing steps for removal of unbound antibodies. This system employed a luciferase (Luc)-conjugated antibody (LucAb) and a dye that absorbed light from the LucAb. The antigen-associated LucAb was localized by transfer of an antigen to the detector-side of a chamber where a detector photomultiplier tube (PMT) was installed. In contrast, the free LucAb was distributed throughout the solution, and the light emitted by the free LucAb was absorbed by the dye...
July 22, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28743655/negative-chemotaxis-of-ralstonia-pseudosolanacearum-to-maleate-and-identification-of-the-maleate-chemosensory-protein
#14
Mattana Tunchai, Akiko Hida, Shota Oku, Yutaka Nakashimada, Toshiyuki Nikata, Takahisa Tajima, Junichi Kato
Ralstonia pseudosolanacearum Ps29 was repelled by maleate. Screening of a complete collection of Ps29 single-methyl-accepting chemotaxis protein (mcp) gene mutants identified the RSp0303 homolog (McpP) as a chemotaxis sensor mediating negative chemotaxis to maleate. Interestingly, the mcpP-deletion mutant was attracted to maleate, indicating that this bacterium expresses a MCP(s) for both positive and negative chemotaxis to maleate. We constructed a Ps29 derivative (designated POC14) harboring deletions in 14 individual mcp genes, including mcpP, to characterize McpP...
July 22, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28751127/isolation-of-six-novel-7-oxo-or-urso-type-secondary-bile-acid-producing-bacteria-from-rat-cecal-contents
#15
Sarinya Tawthep, Satoru Fukiya, Ja-Young Lee, Masahito Hagio, Yoshitoshi Ogura, Tetsuya Hayashi, Atsushi Yokota
Understanding the dynamics of secondary bile acid (SBA) formation in the gut by SBA-producing bacteria is important for host health, as SBAs have been shown to affect host pathophysiology and gut microbiota composition. However, our knowledge of SBA producers is limited in light of the diversity of gut microbes. Here, we isolated six novel SBA-producing bacteria from rat cecal contents, all of which were members of known species of gut microbes. Anaerostipes caccae D10, Bacteroides nordii C5, Clostridioides difficile D7, and Clostridium cadaveris G11 were capable of oxidizing cholic acid and chenodeoxycholic acid into 7-oxo-derivatives with varying yields...
July 21, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28736147/novel-multifunctional-plant-growth-promoting-bacteria-in-co-compost-of-palm-oil-industry-waste
#16
Clament Fui Seung Chin, Yoshihide Furuya, Mohd Huzairi Mohd Zainudin, Norhayati Ramli, Mohd Ali Hassan, Yukihiro Tashiro, Kenji Sakai
Previously, a unique co-compost produced by composting empty fruit bunch with anaerobic sludge from palm oil mill effluent, which contributed to establishing a zero-emission industry in Malaysia. Little was known about the bacterial functions during the composting process and fertilization capacity of this co-compost. We isolated 100 strains from the co-compost on 7 types of enumeration media and screened 25 strains using in vitro tests for 12 traits, grouping them according to three functions: plant growth promoting (fixation of nitrogen; solubilization of phosphorus, potassium, and silicate; production of 3-indoleacetic acid, ammonia, and siderophore), biocontrolling (production of chitinase and anti-Ganoderma activity), and composting (degradation of lignin, xylan, and cellulose)...
July 20, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28734701/highly-chemoselective-and-efficient-production-of-2-6-difluorobenzamide-using-rhodococcus-ruber-cgmcc3090-resting-cells
#17
Rui Tang, Yanbing Shen, Min Wang, Ying Zhai, Qian Gao
Chemoselective biocatalytic hydrolysis of nitriles is a valuable alternative to chemical hydrolysis that operates under harsh conditions. 2,6-Difluorobenzamide (DFBAM) is an essential intermediate derived from synthesis of benzoyl urea insecticide from 2,6-difluorobenzonitrile (DFBN). High yield of DFBAM was achieved, and the method using resting cells of Rhodococcus ruber CGMCC3090 exhibited excellent product specificity. The reaction parameters for DFBAM biosynthesis were also investigated. The resting cells effectively converted DFBN at high concentrations of up to 3...
July 19, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28728975/continuous-nitrogen-removal-by-a-single-stage-reactor-packed-with-ring-laced-string-medium
#18
Masahiro Tatara, Shu Ishikawa, Yoshiyuki Ueno
The efficiency of nitrogen removal by a partial-nitritation/anammox (PNA) reaction was investigated using a packed-bed reactor in which ring-laced strings were used as the supporting medium. A stable population of PNA microorganisms was established from typical activated sludge, after less than two months of acclimation in the packed-bed reactor, by applying a high nitrogen-loading rate (NLR: 0.53 kg/m(3)/d) and short hydraulic retention time (HRT: 1.8 h). The stability of reactor performance was confirmed in industrial wastewater (IW), demonstrating a nitrogen removal efficiency (NRE) of greater than 77% during 260 days of continuous operation, between 0...
July 17, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28728974/activation-of-cryptic-phthoxazolin-a-production-in-streptomyces-avermitilis-by-the-disruption-of-autoregulator-receptor-homologue-avar3
#19
Dian Anggraini Suroto, Shigeru Kitani, Kiyoko T Miyamoto, Yasuko Sakihama, Masayoshi Arai, Haruo Ikeda, Takuya Nihira
The genomes of actinomycetes encode many cryptic novel/useful bioactive compounds, but access to these cryptic secondary metabolites remains limited. Streptomyces avermitilis predominantly produces three polyketide antibiotics (avermectin, filipin, and oligomycin) but has the potential to produce more secondary metabolites based on the number of cryptic biosynthetic gene clusters. Here, we extensively investigated the metabolite profiles of a gene disruptant of AvaR3 (an autoregulator receptor homologue), which is involved in the pleiotropic regulation of antibiotic production and cell morphology...
July 17, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28716629/characterization-of-novel-bacillus-strain-n31-from-mariculture-water-capable-of-halophilic-heterotrophic-nitrification-aerobic-denitrification
#20
Fei Huang, Luqing Pan, Na Lv, Xianming Tang
The development of an intensive aquaculture industry has been accompanied by increasing environmental impacts, especially nitrogen pollution. In this study, a novel halophilic bacterium capable of heterotrophic nitrification and aerobic denitrification was isolated from mariculture water and identified as Bacillus litoralis N31. The efficiency of ammonium, nitrite and nitrate removal by N31 were 86.3%, 89.3% and 89.4%, respectively, after a 48-h cultivation in sole N-source medium with initial nitrogen approximately 20 mg/L...
July 14, 2017: Journal of Bioscience and Bioengineering
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