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Journal of Bioscience and Bioengineering

Wei Zheng, Teng Cai, Manhong Huang, Donghui Chen
Microbial fuel cells (MFCs) have attracted intensive interest for their power generation and pollutants removal characteristics. Electrochemical performances and community structures of two algae cathode photosynthetic MFCs were investigated and compared. Microbial consortia of these two MFCs were taken from wetland sediment (named SMFC) and an up-flow anaerobic wastewater treatment reactor (named UMFC). Maximum power density of the SMFC and UMFC achieved 202.9 ± 18.1 mW/m(2) and 158.2±15.1 mW/m(2), respectively...
June 15, 2017: Journal of Bioscience and Bioengineering
Jun Lv, Bo-Bo Zhang, Xiao-Dong Liu, Chan Zhang, Lei Chen, Gan-Rong Xu, Peter Chi Keung Cheung
Natural yellow pigments produced by submerged fermentation of Monascus purpureus have potential economic value and application in the food industry. In the present study, the relationships among fermentation conditions (in terms of pH and shaking/agitation speed), mycelial morphology and the production of Monascus yellow pigments were investigated in both shake-flask and scale-up bioreactor experiments. In the shake-flask fermentation, the highest yield of the Monascus yellow pigments was obtained at pH 5.0 and a shaking speed of 180 rpm...
June 15, 2017: Journal of Bioscience and Bioengineering
Xue-Jiao Li, Jian-Wei Dong, Le Cai, Rui-Feng Mei, Zhong-Tao Ding
Illigera henryi, an endemic traditional Chinese medicine, contains abundant aporphine alkaloids that possess various bioactivities. In the present study, tubers of I. henryi were fermented by several fungi, and the acetylcholinesterase (AChE) inhibitory activities of non-fermented and fermented I. henryi were measured. The results showed that the fermentation of I. henryi with Clonostachys rogersoniana 828H2 is effective for improving the AChE inhibitory activity. A key biotransformation was found during the C...
June 13, 2017: Journal of Bioscience and Bioengineering
Shigekuni Noba, Nana Yako, Minoru Kobayashi, Susumu Masuda, Tetsuya Watanabe
Onion-like off-flavor is a highly undesirable property in beer. Although several compounds that impart onion-like odors have been identified, the individual contribution of these compounds to the onion-like off-flavor in beer is not clear. In the present study, we searched for compounds that impart an onion-like odor by gas chromatography (GC)-olfactometry. The analysis of several types of beer revealed that 2-mercapto-3-methyl-1-butanol (2M3MB) and 3-mercapto-3-methyl-1-butanol (3M3MB) were possible causative compounds...
June 8, 2017: Journal of Bioscience and Bioengineering
Masayuki Takahashi, Yasuko Kita, Akihiro Mizuno, Nami Goto-Yamamoto
Various methods are used for analyzing a bacterial community. We recently developed a method for quantifying each bacterium constituting the microbiota by combining a next-generation sequencing (NGS) analysis with a quantitative polymerase chain reaction (NGS-qPCR) assay. Our NGS-qPCR method is useful for analyzing a comprehensive bacterial community because it is enables the easy calculation of the amounts of each bacterium constituting the microbiota. However, it has not been confirmed whether the estimated bacterial community obtained using this NGS-qPCR method corresponds to the results obtained using conventional methods...
June 7, 2017: Journal of Bioscience and Bioengineering
Kamal Prashad Segar, Vikas Chandrawanshi, Sarika Mehra
Process engineering to improve product quality and titers is gaining importance at late-stage cell culture process development. Valproic acid, a US Food and Drug Administration-approved histone deacetylase (HDAC) inhibitor, has been shown to improve cell culture performance with higher productivities and minimal effect on the product quality. However, the wider physiological impact of valproic acid on recombinant cells has not been investigated till date. In this study, we investigate the role of unfolded protein response pathway when immunoglobulin G (IgG)-secreting Chinese hamster ovary (CHO) cells are treated with valproic acid, resulting in a 3-fold increase in product titers and productivity...
June 7, 2017: Journal of Bioscience and Bioengineering
Chiu-Yue Lin, Chyi-How Lay, I-Yuan Sung, Biswarup Sen, Chin-Chao Chen
The cultivation of mushrooms generates large amounts of waste polypropylene bags stuffed with wood flour and bacterial nutrients that makes the mushroom waste (MW) a potential feedstock for anaerobic bioH2 fermentation. MW indigenous bacteria were enriched using thermophilic temperature (55°C) for use as the seed inoculum without any external seeding. The peak hydrogen production rate (6.84 mmol H2/L-d) was obtained with cultivation pH 8 and substrate concentration of 60 g MW/L in batch fermentation. Hydrogen production yield (HY) is pH and substrate concentration dependent with an HY decline occurring at pH and substrate concentration increasing from pH 8 to 10 and 60 to 80 g MW/L, respectively...
June 7, 2017: Journal of Bioscience and Bioengineering
Suman C Nath, Masanobu Horie, Eiji Nagamori, Masahiro Kino-Oka
Aggregate culture of human induced pluripotent stem cells (hiPSCs) is a promising method to obtain high number of cells for cell therapy applications. This study quantitatively evaluated the effects of initial cell number and culture time on the growth of hiPSCs in the culture of single aggregate. Small size aggregates ((1.1 ± 0.4) × 10(1)-(2.8 ± 0.5) × 10(1) cells/aggregate) showed a lower growth rate in comparison to medium size aggregates ((8.8 ± 0.8) × 10(1)-(6.8 ± 1.1) × 10(2) cells/aggregate) during early-stage of culture (24-72 h)...
June 7, 2017: Journal of Bioscience and Bioengineering
Yutaka Konya, Moyu Taniguchi, Eiichiro Fukusaki
No abstract text is available yet for this article.
June 3, 2017: Journal of Bioscience and Bioengineering
Xiaoju Chen, Xuefeng Wu, Shaotong Jiang, Xingjiang Li
Environmental conditions, particularly pH, have significant effects on the efficiency and final titers of bio-based products. Therefore, these factors need to be identified to ensure the fermentation process is economically attractive. In this study, strategies for controlling pH were optimized to enhance succinic acid production by Corynebacterium crenatum J-2. The results indicate that pH 6.8 is the optimal value for anaerobic succinic acid production by C. crenatum J-2 in terms of productivity and titer...
June 2, 2017: Journal of Bioscience and Bioengineering
Yoshitaka Saga, Hayato Yamashita
Metabolic substitution of the esterifying chain in bacteriochlorophyll (BChl) c in green photosynthetic bacteria grown by supplementation of exogenous alcohols has attracted attentions to study supramolecular structures and biogenesis of major antenna complexes chlorosomes in these bacteria as well as BChl pigment biosynthesis. Actual substrates in the enzymatic attachment of the esterifying moieties to the precursor of BChl c, namely bacteriochlorophyllide (BChlide) c, in these bacteria are believed to be diphosphate esters of alcoholic substrates, although only intact alcohols have so far been supplemented in the bacterial cultures...
May 31, 2017: Journal of Bioscience and Bioengineering
Moyu Taniguchi, Yutaka Konya, Yosuke Nakano, Eiichiro Fukusaki
Although naturally abundant amino acids are represented mainly by l-enantiomers, fermented foods are known to contain various d-amino acids. Enantiospecific profiles of food products can vary due to fermentation by bacteria, and such alterations may contribute to changes in food properties that would not be dependent exclusively on l-amino acids. Therefore, more attention should be paid to the study of temporal alterations of d-amino acid profiles during fermentation process. However, there have been very few studies reporting time-dependent profiling of d-amino acids because enantioseparation of widely targeted d-amino acids is technically difficult...
May 26, 2017: Journal of Bioscience and Bioengineering
Yukinori Tanaka, Ken Kasahara, Yutaka Hirose, Yu Morimoto, Masumi Izawa, Kozo Ochi
Ribosome engineering, originally applied to Streptomyces lividans, has been widely utilized for strain improvement, especially for the activation of bacterial secondary metabolism. This study assessed ribosome engineering technology to modulate primary metabolism, taking butanol production as a representative example. The introduction into Clostridium saccharoperbutylacetonicum of mutations conferring resistance to butanol (But(R)) and of the str mutation (Sm(R); a mutation in the rpsL gene encoding ribosomal protein S12), conferring high-level resistance to streptomycin, increased butanol production 1...
May 26, 2017: Journal of Bioscience and Bioengineering
Burcu Turanlı-Yıldız, Laurent Benbadis, Ceren Alkım, Tuğba Sezgin, Arman Akşit, Abdülmecit Gökçe, Yavuz Öztürk, Ahmet Tarık Baykal, Zeynep Petek Çakar, Jean M François
Microbial ethanol production is an important alternative energy resource to replace fossil fuels, but at high level, this product is highly toxic, which hampers its efficient production. Towards increasing ethanol-tolerance of Saccharomyces cerevisiae, the so far best industrial ethanol-producer, we evaluated an in vivo evolutionary engineering strategy based on batch selection under both constant (5%, v v(-1)) and gradually increasing (5-11.4%, v v(-1)) ethanol concentrations. Selection under increasing ethanol levels yielded evolved clones that could tolerate up to 12% (v v(-1)) ethanol and had cross-resistance to other stresses...
May 25, 2017: Journal of Bioscience and Bioengineering
Guanhua Li, Xiaoxue Liu, Lin Yuan
A novel strategy of enhancing laccase production by absorbent fermentation was investigated. Peanut shell was used as nutrient carrier for laccase production by Funalia trogii IFP0027 in the absorbent fermentation. The maximum laccase production was reached to 11,900 U/l, which was 4.97 times higher than that of the control group. The results indicated that carbohydrates and phenolic substances especially flavonoids contained in peanut shell stimulated laccase production by F. trogii. Meanwhile, the peanut shell nutrient carrier could not only alleviate the oxidative damage, owing to strong scavenging activity on hydroxyl, but also relieve the mechanical stresses to form small and regular microbial pellets...
May 22, 2017: Journal of Bioscience and Bioengineering
Keisuke Morita, Yuta Nomura, Jun Ishii, Fumio Matsuda, Akihiko Kondo, Hiroshi Shimizu
Bacterial phosphoenol pyruvate carboxylase (PPC) and enzymes in the Entner-Doudoroff (ED) pathway were heterologously expressed in Saccharomyces cerevisiae to improve the NADPH supply required for the bio-production of chemicals such as isobutanol. The heterologous expression of PPC from Synechocystis sp. PCC6803 increased in the isobutabol titer 1.45-fold (93.2±1.6 mg/L) in metabolically engineered S. cerevisiae strains producing isobutanol. This result suggested that the pyruvate and NADPH supply for isobutanol biosynthesis was activated by PPC overexpression...
May 20, 2017: Journal of Bioscience and Bioengineering
Ivy Grace Umadhay Pait, Shigeru Kitani, Yohanes Novi Kurniawan, Maeda Asa, Takashi Iwai, Haruo Ikeda, Takuya Nihira
Streptomyces lavendulae FRI-5 produces the blue pigment indigoidine and other secondary metabolites (d-cycloserine and nucleoside antibiotics). The production of these useful compounds is controlled by a signaling cascade mediated by the γ-butyrolactone autoregulator IM-2. Previously we revealed that the far regulatory island includes the IM-2 receptor, the IM-2 biosynthetic enzyme, and several transcriptional regulators, and that it contributes to the regulation of indigoidine production in response to the signaling molecule...
May 20, 2017: Journal of Bioscience and Bioengineering
Daichi Nakajima, Toshiyuki Shibata, Reiji Tanaka, Kouichi Kuroda, Mitsuyoshi Ueda, Hideo Miyake
Clostridium cellulovorans 743B, an anaerobic and mesophilic bacterium, produces an extracellular enzyme complex called the cellulosome on the cell surface. Recently, we have reported the whole genome sequence of C. cellulovorans, which revealed that a total of 4 cellulosomal scaffolding proteins: CbpA, HbpA, CbpB, and CbpC were encoded in the C. cellulovorans genome. In particular, cbpC encoded a 429-residue polypeptide that includes a carbohydrate-binding module (CBM), an S-layer homology module, and a cohesin...
May 19, 2017: Journal of Bioscience and Bioengineering
Qinzheng Zhou, Peng Ji, Jianye Zhang, Xue Li, Chao Han
A novel thermostable endoglucanase (CTendo45) encoding gene was cloned from Chaetomium thermophilum and heterologously expressed in Pichia pastoris. Sequence alignment indicated that the CTendo45 enzyme belonged to glycoside hydrolase family 45. The recombinant enzyme was purified by Ni(2+) affinity chromatography, and its apparent molecular mass was estimated to be 32 kDa by SDS-PAGE. The purified enzyme displayed maximum activity at 70°C and pH 4. CTendo45 was stable at 60°C for 1 h, and residual activities of 78...
May 19, 2017: Journal of Bioscience and Bioengineering
Parthiban Anburajan, Jong-Hun Park, Periyasamy Sivagurunathan, Arivalagan Pugazhendhi, Gopalakrishnan Kumar, Chang-Su Choi, Sang-Hyoun Kim
This study examined the mesophilic continuous biohydrogen fermentation from galactose and glucose mixture with an initial substrate concentration of 15 g/L (galactose 12 g/L and glucose 3 g/L) as a resembling carbon source of pretreated red algal hydrolyzate. A fixed bed reactor was fed with the sugar mixture at various hydraulic retention times (HRTs) ranging 12 to 1.5 h. The maximum hydrogen production rate of 52.6 L/L-d was found at 2 h HRT, while the maximum hydrogen yield of 2.3±0.1 mol/mol hexoseadded, was achieved at 3 h HRT...
May 18, 2017: Journal of Bioscience and Bioengineering
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