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Journal of Bioscience and Bioengineering

Li Jiang, Wenjun Xiao, Xuan Zhou, Weiyu Wang, Jun Fan
In this study, we analyzed and compared the properties of yeast Ulp1 protease in active inclusion bodies (IBs) as special protein immobilizate, and the soluble Ulp1 via oriented immobilization. Fusion of the N-terminal self-assembling peptide GFIL8 to the Ulp1 increased production of active IBs in Escherichia coli. Attachment of the N-terminal cellulose-binding module facilitated the constructed protein immobilized on the regenerated amorphous cellulose (RAC) with a binding capacity up to about 235 mg protein per gram of RAC...
July 10, 2018: Journal of Bioscience and Bioengineering
Ana Isabel Díaz, Paula Oulego, Sergio Collado, Adriana Laca, José Manuel González, Mario Díaz
Sewage sludge can be treated by anaerobic processes that frequently are followed by physical separation processes. In this work, a high-throughput sequencing technology, based on variation in the bacterial 16S rRNA gene, has been used to characterise the bacterial populations present in samples taken from different points of an industrial anaerobic digestion process fed with sewage sludge. Relative abundances of phyla and classes throughout the biological process and the subsequent separation steps were determined...
July 10, 2018: Journal of Bioscience and Bioengineering
Koichi Toyoda, Masaharu Ishii, Hiroyuki Arai
The obligate chemolithoautotrophic bacterium, Hydrogenovibrio marinus MH-110 has three ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) isoenzymes, designated CbbLS-1, CbbLS-2, and CbbM, which are encoded by the cbbL1S1, cbbL2S2, and cbbM genes, respectively. Functions of these isoenzymes at different CO2 concentrations were investigated using deletion mutants of their genes. Deletion of cbbL1 had no effect on cell growth under any of the test growth conditions. The cbbL2 mutant was unable to grow under lower (≤0...
June 27, 2018: Journal of Bioscience and Bioengineering
Song Xia, Baoyan Gao, Jiaqi Fu, Jihai Xiong, Chengwu Zhang
Microalgae are recognized as promising producers of many bioactive products, but their utility is limited due to high production costs. We subjected the marine diatom Odontella aurita to three nitrogen supply regimes [initial low nitrogen (ILN), initial high nitrogen (IHN), and initial high nitrogen plus supplementary nitrogen (SN)] to investigate the accumulation of three high-value bioactive components: fucoxanthin, chrysolaminarin and eicosapentaenoic acid (EPA). We found that SN conditions maximized fucoxanthin accumulation: a maximum productivity of 6...
June 27, 2018: Journal of Bioscience and Bioengineering
Daisuke Koma, Takahiro Kishida, Hayato Yamanaka, Kunihiko Moriyoshi, Eiji Nagamori, Takashi Ohmoto
Many metabolic engineering approaches have been attempted to generate strains capable of producing valuable compounds. One of main goals is industrial application of these strains. Integration of synthetic pathway genes into the Escherichia coli chromosome enables generation of a plasmid-free strain that is stable and useful for industrial applications. Strains that do not require induction are advantageous in terms of cost. In the present study, we constructed a constitutive overexpression system in E. coli to generate plasmid-free and inducer-free strains...
June 26, 2018: Journal of Bioscience and Bioengineering
Peiyuan Jing, Xi Cao, Xinyao Lu, Hong Zong, Bin Zhuge
1,2,4-Butanetriol (BT) is an important precursor of energetic material 1,2,4-butanetriol trinitrate (BTTN) in military field. Here, the recombinant Escherichia coli was modified by multi-strategy to increase BT production. First, the 2-keto acid reduction pathway was blocked by double deleting the genes yiaE and ycdW, resulting in 19% and 41% increase of BT titer and yield, respectively. Further disruption of xylose branch pathway by deleting xylA gene led to a 24% increase of BT yield, but the biomass and BT titer were decreased by 63% and 55%, respectively...
June 23, 2018: Journal of Bioscience and Bioengineering
Rei Miyano, Hirotaka Matsuo, Kenichi Nonaka, Takayuki Mokudai, Yoshimi Niwano, Kazuro Shiomi, Yōko Takahashi, Satoshi Ōmura, Takuji Nakashima
New natural products, designated pochoniolides A and B, were isolated from the cultured broth of fungal strain FKI-7537 using a physicochemical screening methodology. Strain FKI-7537 was isolated from a soil sample collected at Niijima, Tokyo, Japan and identified as Pochonia chlamydosporia var. spinulospora by morphological characteristics and DNA sequence analysis. The chemical structures of pochoniolides A and B were elucidated by NMR and mass spectra and found to be new compounds consisting of a muconolactone moiety connected with a chromone unit...
June 22, 2018: Journal of Bioscience and Bioengineering
Ghoson Mosbah Daba, Naoki Ishibashi, Xiao Gong, Hiroya Taki, Keisuke Yamashiro, Yen Yi Lim, Takeshi Zendo, Kenji Sonomoto
Lactococcin Z is a novel Lactococcus-specific bacteriocin produced by Lactococcus lactis QU 7 that shares 55.6% identity with lactococcin A. To identify the receptor targeted by lactococcin Z, several lactococcin Z-resistant mutants were generated from the sensitive strain, L. lactis IL1403. The resistant mutants showed difficulties in utilising mannose and glucose as sole carbon sources, contrary to their pattern of growth in the presence of galactose as a sole carbon source. Mutations were found in the ptnC and ptnD genes of lactococcin Z-resistant mutants, which encode the mannose phosphotransferase system (Man-PTS) components, IIC and IID, respectively; therefore, IIC and IID are proposed as potential receptors employed by lactococcin Z and are the same receptors targeted by lactococcin A...
June 18, 2018: Journal of Bioscience and Bioengineering
Mayu Oomuro, Daisuke Watanabe, Yukiko Sugimoto, Taku Kato, Yasuo Motoyama, Tetsuya Watanabe, Hiroshi Takagi
High-gravity brewing has been used to reduce costs and energy, as well as to produce new types of beer with high alcohol content. To identify the key metabolic pathways underlying efficient high-gravity brewing, we explored metabolites that were highly accumulated during alcoholic fermentation under high-maltose conditions using bottom-fermenting brewer's yeast, Saccharomyces pastorianus. Based on metabolomic data, we focused on S-adenosylmethionine (SAM), which may be involved in glycolysis and alcoholic fermentation in the closely related yeast species Saccharomyces cerevisiae...
June 16, 2018: Journal of Bioscience and Bioengineering
Takayuki Takei, Shogo Sakoguchi, Masahiro Yoshida
Magnetic manipulation of paramagnetic particles had great potential for efficient bioprocessing. In this study, we stirred microliter-volume water droplets formed on a superhydrophobic surface as micro-bioreactors by using paramagnetic magnetite microparticles manipulated by an external magnetic field. We showed that magnetite microparticles in the droplets spontaneously formed rod-like aggregates, which were like commercial stir bars, in an external magnetic field and spun with rotating of magnetic field. Increasing the rotating rate of the magnetic field and increasing the concentration of the microparticles caused the microparticles to fixate at the air/water interface of the droplets and their rotation at the interface with rotating of magnetic field...
June 15, 2018: Journal of Bioscience and Bioengineering
Haoxin Lv, Akio Tani
Oharaeibacter diazotrophicus strain SM30T , isolated from rice rhizosphere, is an aerobic, facultative lanthanide (Ln3+ )-utilizing methylotroph and diazotroph that belongs to the Methylocystaceae family. In this research, the complete genome sequence of strain SM30T was determined, and its methylotrophy modules were characterized. The genome consists of one chromosome and two plasmids, comprising a total of 5,004,097 bp, and the GC content was 71.6 mol%. A total of 4497 CDSs, 67 tRNA, and 9 rRNA were encoded...
June 15, 2018: Journal of Bioscience and Bioengineering
Yuki Miyahara, Mino Oota, Takeharu Tsuge
Acetoacetyl-CoA reductase (PhaB), involved in poly(3-hydroxybutyrate) [P(3HB)] biosynthesis, requires the coenzyme NADPH as a reducing agent. In this study, the effect of NADPH supply on P(3HB) production was investigated in vitro and in vivo using a phosphite dehydrogenase double mutant (PtxDEAAR ), which catalyzes oxidation of phosphite to phosphate with the generation of NADH and NADPH. In an in vitro assay using purified enzymes, P(3HB) polymerization was observed only when phosphite and PtxDEAAR were present, confirming that NADPH was supplied to PhaB...
June 15, 2018: Journal of Bioscience and Bioengineering
Hongyan Liu, Haihua Qin, Hongyu Wang
This study aimed to investigate the characteristics of hydrogen production by mixed cultures using Laminaria japonica hydrolysates. The hydrolysates of L. japonica were prepared by pretreatment methods, including heat (100°C or 121°C) and acid (HCl or H2 SO4 ) pretreatments. The mixed cultures could produce hydrogen using L. japonica as a substrate, with the highest cumulative hydrogen production of 825 ± 14 mL/L from HCl-pretreated L. japonica. High-throughput sequencing of the 16S rRNA gene revealed that the microbial community in the hydrolysate of HCl-pretreated L...
June 15, 2018: Journal of Bioscience and Bioengineering
Yoshiko Fujitani, Kabir Md Alamgir, Akio Tani
Ergothioneine (EGT) is a sulfur-containing, anti-oxidative amino acid derived from histidine. EGT is synthesized in bacteria and fungi but not in animals and plants, and is now recognized as important for human health. Its cost-effective fermentative production has not been elucidated due to the lack of information for productive microorganisms. In this study, we doubled the gene copy for EGT synthesis and deleted the histidine ammonia-lyase gene in a potent EGT-producing methylotrophic bacterium Methylobacterium aquaticum strain 22A, and optimized its culture conditions, resulting in increased EGT production of 7...
June 14, 2018: Journal of Bioscience and Bioengineering
Jiun-Yan Wu, Jen-Hung Liao, Chwen-Jen Shieh, Feng-Chia Hsieh, Yung-Chuan Liu
In this study, the precursor effect for iturin A production was quantitatively analyzed. A strain identified as Bacillus amyloliquefaciens BPD1 (Ba-BPD1) was selected due to its ability to produce iturin A. The enhancement of iturin A production in a submerged culture was tested using various additives, including palmitic acid, oils, and complex amino acids. Among these, complex amino acids triggered the highest yield at 559 mg/L. The respective amino acids that contribute to the structure of iturin A were used as precursors...
June 12, 2018: Journal of Bioscience and Bioengineering
Tatsuhiko Nagao, Yoshihito Arai, Mika Yamaoka, Fumika Komatsu, Hisashi Yagi, Hirokazu Suzuki, Takashi Ohshiro
Fucoidan is a hetero-sulfated polysaccharide found in brown algae and has received much attention as an ingredient in functional and health foods. The marine bacterial strain Luteolibacter algae H18 degrades fucoidan from Cladosiphon okamuranus. We purified the fucoidanase from a cell-free extract of L. algae H18, used it to decrease the molecular weight of deacetylated-fucoidan, determined the N-terminal amino acid sequence of the enzyme, and identified the gene involved in the degradation of fucoidan, fct114, in a draft genome sequence of strain H18...
June 12, 2018: Journal of Bioscience and Bioengineering
Kenichi Ogawa, Katsunori Yoshikawa, Fumio Matsuda, Yoshihiro Toya, Hiroshi Shimizu
Photoinhibition, or cell damage caused by excessively intense light is a major issue for the industrial use of cyanobacteria. To investigate the mechanism of responses to extreme high light intensity, gene expression analysis was performed using the model cyanobacterium Synechocystis sp. PCC 6803 (PCC 6803) cultured under various light intensities. The culture profile data demonstrated that, in contrast to the slow cell growth observed under low light intensities (30 and 50 μmol m-2  s-1 ), maximal cell growth was observed under mid light conditions (300 and 1000 μmol m-2  s-1 )...
June 12, 2018: Journal of Bioscience and Bioengineering
Dani Permana, Kosuke Minamihata, Masahiro Goto, Noriho Kamiya
The site-specific cross-linking of functional proteins creates macromolecular assemblies that exhibit unique biochemical and/or physicochemical properties. Herein, we explored the potential of laccase as a biocatalyst for the site-specific cross-linking of tyrosine-tagged proteins. Trametes sp. laccase (TL) was selected as the cross-linking catalyst, and Escherichia coli alkaline phosphatase (BAP) and antibody-binding proteins (pG2 pAs) were employed as model proteins. The protein models were genetically fused to a peptide tag containing a tyrosine residue (Y-tag) at the N- and/or C-termini...
June 12, 2018: Journal of Bioscience and Bioengineering
Mayu Shibuta, Masato Tamura, Kei Kanie, Masumi Yanagisawa, Hirofumi Matsui, Taku Satoh, Toshiyuki Takagi, Toshiyuki Kanamori, Shinji Sugiura, Ryuji Kato
Cellular morphology on and in a scaffold composed of extracellular matrix generally represents the cellular phenotype. Therefore, morphology-based cell separation should be interesting method that is applicable to cell separation without staining surface markers in contrast to conventional cell separation methods (e.g., fluorescence activated cell sorting and magnetic activated cell sorting). In our previous study, we have proposed a cloning technology using a photodegradable gelatin hydrogel to separate the individual cells on and in hydrogels...
June 9, 2018: Journal of Bioscience and Bioengineering
Norihisa Haraguchi, Jun Kaseda, Yasumune Nakayama, Kazuhiro Nagahama, Takahira Ogawa, Masayoshi Matsuoka
Photosystem II complex embedded in thylakoid membrane performs oxygenic photosynthesis where the reaction center D1/D2 heterodimer accommodates all components of the electron transport chain. To express thermostable D1/D2 heterodimer in a cyanobacterium Synechococcus elongatus PCC 7942, we constructed a series of mutant strains whose psbA1 and psbD1 genes encoding, respectively, the most highly expressed D1 and D2 polypeptides were replaced with those of a thermophilic strain, Thermosynechococcus vulcanus. Because the C-terminal 16 amino acid sequences of D1 polypeptides should be processed prior to maturation but diverge from each other, we also constructed the psbA1ΔC-replaced strain expressing a thermostable D1 polypeptide devoid of the C-terminal extension...
June 8, 2018: Journal of Bioscience and Bioengineering
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