journal
MENU ▼
Read by QxMD icon Read
search

Applied Immunohistochemistry & Molecular Morphology: AIMM

journal
https://www.readbyqxmd.com/read/28099174/discordance-rate-in-estrogen-receptor-progesterone-receptor-her2-status-and-ki67-index-between-primary-unifocal-and-multiple-homogenous-breast-carcinomas-and-synchronous-axillary-lymph-node-metastases-have-an-impact-on-therapeutic-decision
#1
Rares Georgescu, Monica Boros, Denisa Moncea, Orsolya Bauer, Marius-Florin Coros, Adela Oprea, Cosmin Moldovan, Cristian Podoleanu, Simona Stolnicu
BACKGROUND: We aimed to demonstrate that in breast carcinomas the tumor profile is not stable during the metastatic process, with impact on therapeutic decisions. MATERIALS AND METHODS: We analyzed the estrogen receptor (ER), progesterone receptor (PR), and HER2 status and Ki67 index in 41 primary unifocal (PU) and 37 primary multiple (PM) breast carcinomas with identical immunohistochemical profiles among multiple tumor foci and the matched axillary lymph node metastases...
January 17, 2017: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/28059871/modified-nanoantibodies-increase-sensitivity-in-avidin-biotin-immunohistochemistry
#2
Anthony Wong, Chelsea Sykora, Lewis Rogers, Jennifer Higginbotham, Jiwu Wang
Revealing the spatial arrangement of molecules within a tissue through immunohistochemistry (IHC) is an invaluable tool in biomedical research and clinical diagnostics. Choosing both the appropriate antibody and amplification system is paramount to the pathologic interpretation of the tissue at hand. The use of single domain VHH nanoantibodies (nAbs) promise more robust and consistent results in IHC, but are rarely used as an alternative to conventional immunoglobulin G (IgG) antibodies. nAbs are originally obtained from llamas and are the smallest antigen-binding fragments available...
January 4, 2017: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/28027119/comparison-of-%C3%AE-catenin-and-lef1-immunohistochemical-stains-in-desmoid-type-fibromatosis-and-its-selected-mimickers-with-unexpected-finding-of-lef1-positivity-in-scars
#3
Youran Zou, Yaxia Zhang, James Church, Xiuli Liu
β-catenin immunohistochemical stain can be useful in the diagnosis of many tumors including desmoid-type fibromatosis (DTF). Lymphoid enhancer-factor 1 (LEF1), a recently emerged marker, is part of the Wnt pathway with β-catenin but has not been studied in DTF. We performed LEF1 and β-catenin immunohistochemistry in DTF (n=26), superficial fibromatosis (n=19), sclerosing mesenteritis (n=12), gastrointestinal stromal tumor (n=17), and cutaneous scar (n=14) using tissue microarray and whole sections. The staining intensity was scored as strong (visible at ×2 objective, value of 3), moderate (visible at ×4, value of 2), weak (visible at ×10, value of 1), and negative (not visible at ×10, value of 0)...
December 23, 2016: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/28027118/gain-of-tp53-mutation-in-imatinib-treated-sdh-deficient-gastrointestinal-stromal-tumor-and-clinical-utilization-of-targeted-next-generation-sequencing-panel-for-therapeutic-decision-support
#4
Christina H Wei, Jonas Pettersson, Mihaela Campan, Shefali Chopra, Wesley Naritoku, Sue E Martin, Pamela M Ward
Patients with succinate dehydrogenase (SDH)-deficient gastrointestinal stromal tumor (GIST) have few therapeutic options. Despite lack of KIT or platelet-derived growth factor receptor A (PDGFRA) driver mutations, SDH-deficient GISTs display strong expression of KIT by immunohistochemistry and these patients are often treated with tyrosine kinase inhibitors, including imatinib as a first-line therapy. Using a targeted next-generation sequencing panel of mutation hotspots of 50-clinically relevant genes, we investigated (1) concurrence of somatic/actionable mutations and (2) tumor molecular evolution by comparing 2 resection specimens 1...
December 23, 2016: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/28027117/precision-medicine-starts-with-preanalytics-real-time-assessment-of-tissue-fixation-quality-by-ultrasound-time-of-flight-analysis
#5
Melissa L Lerch, Daniel R Bauer, David Chafin, Abbey Theiss, Michael Otter, Geoffrey S Baird
Personalized medicine promises diagnosis and treatment of disease at the individual level and relies heavily on clinical specimen integrity and diagnostic assay quality. Preanalytics, the collection and handling steps of a clinical specimen before immunohistochemistry or other clinical assay, are critically important to enable the correct diagnosis of disease. However, the effects of preanalytics are often overlooked due to a lack of standardization and limited assessment tools to quantify their variation. Here, we report a novel real-time ultrasound time-of-flight instrument that is capable of monitoring and imaging the critical step in formalin fixation, diffusion of the fixative into tissue, which provides a quantifiable quality metric for tissue fixation in the clinical laboratory ensuring consistent downstream molecular assay results...
December 23, 2016: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/27997502/mitotic-index-determined-by-phosphohistone-h3-immunohistochemistry-for-precise-grading-in-follicular-lymphoma
#6
Judit Bedekovics, Gábor Irsai, Katalin Hegyi, Lívia Beke, László Krenács, Lajos Gergely, Gábor Méhes
The World Health Organization classification recommends follicular lymphoma (FL) grading (G1-3) by considering centroblast number, while also suggesting its influence on disease outcome. As centroblast counting and other proliferation markers have limitations, we looked for more specific measures of cellular activity in FL. Phosphorylated histone H3 (pHH3) was widely applied for the objective detection of mitotic activity in different tumors. The aim was to evaluate the utility of pHH3 protein in FL grading and compare its value with the classical features of cell proliferation...
December 16, 2016: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/27941568/the-family-of-%C3%AE-catenin-ovarian-tumors
#7
Maria C Mengoli, Riccardo Valli, Gian F Zannoni
No abstract text is available yet for this article.
December 9, 2016: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/27941567/a-serous-peritoneal-cancer-revealing-itself-with-double-breast-cancer-metastases-in-2-different-periods
#8
Kemal Beksac, Cigdem Irkkan, Guldeniz Argun, Bahadir Cetin
A 72-year-old woman presented with a mass on the right axilla. This was thought to be an occult breast cancer case, and the patient was treated with modified radical mastectomy, followed by hormonotherapy. Two years later she presented with incarcerated umbilical hernia. Pathology revealed Sister Mary Joseph's nodule inside the hernia sac. Further evaluation revealed that the primary tumor was papillary serous carcinoma of the peritoneal surface. The patient received adjuvant chemotherapy. Two years later the metastatic tumor was located on the other breast...
December 9, 2016: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/27941566/folate-receptor-alpha-expression-in-platinum-resistant-refractory-ovarian-carcinomas-and-primary-endocervical-adenocarcinomas
#9
Lisa A Rubinsak, Cynthia Cohen, Namita Khanna, Ira R Horowitz, Krisztina Z Hanley
INTRODUCTION: Treatment of advanced stage ovarian carcinoma is challenging, and despite surgical treatment and chemotherapy, the 5-year survival rate is estimated around 30%. Early recurrence and resistance to platinum-based chemotherapy are associated with poor prognosis and limited response to available second-line chemotherapy. The relative incidence of endocervical adenocarcinoma (EAC) compared with squamous cell carcinoma is increasing. Although the first-line treatment modality for early stage EAC is surgical resection, for locally advanced disease chemoradiation or neoadjuvant chemotherapy is used...
December 9, 2016: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/27941565/clinicopathologic-correlates-of-helicobacter-pylori-in-gastric-mucosa
#10
Neelam Wadhwa, Sanjay Gupta, Sarla Aggarwal, Kiran Mishra
No abstract text is available yet for this article.
December 9, 2016: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/27941564/paired-box-5-pax5-expression-in-poorly-differentiated-neuroendocrine-carcinoma-of-the-gastrointestinal-and-pancreatobiliary-tract-diagnostic-and-potentially-therapeutic-implications
#11
Sanaz Ainechi, Steven A Mann, Jingmei Lin, Deepa Patil, Christine E Sheehan, Zhaohai Yang, Chunlai Zuo, Hwajeong Lee
Paired Box 5 (PAX5), a well-established B-cell marker, is preferentially expressed in small cell lung carcinoma and regulates the transcription of c-Met, offering a potential for therapeutic target. Its expression in poorly differentiated neuroendocrine carcinoma (PDNEC) of the digestive system has not been systemically evaluated. Archived pathology materials from 38 PDNEC in the gastrointestinal (GI) and pancreatobiliary (PB) tract were reviewed. Representative tumor sections were subject to immunohistochemical stain for PAX5, c-Met, and CD20...
December 9, 2016: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/27941563/the-role-of-collagen-iv-and-cytokeratin-5-6-immunohistochemistry-in-identifying-subtypes-of-hereditary-epidermolysis-bullosa
#12
Ahmed Alhumidi
Hereditary epidermolysis bullosa (EB) constitute a genodermatosis group with variable clinical severity. Biopsies diagnosed as EB in the last 4 years were retrieved from the database of the king Khalid University Hospital and military hospital lab at Saudi Arabia. The current study was performed to examine the diagnostic usefulness of immunohistochemistry, as compared with electron microscopic examination, for subclassification of HEB. Fourteen cases were studied. Collagen IV immunostain was located above the blister in all dystrophic EB cases, and below the blister in all cases of epidermolytic and junctional EB...
December 9, 2016: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/27941562/clear-cell-variant-of-calcifying-epithelial-odontogenic-tumor-a-case-report-and-preliminary-immunohistochemical-study-of-the-shh-pathway
#13
Braúlio C Júnior, Vinicius R V M Muniz, Manuela T A Vidal, Clarissa A Gurgel, Jorge E Leon, Roberto A De Azevedo, Iêda C Rebello, Jean N Dos Santos
No abstract text is available yet for this article.
December 9, 2016: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/27941561/sporadic-burkitt-lymphoma-presenting-as-intestinal-polyposis-in-a-child
#14
Sabah Boudjemaa, Linda Dainese, Joseph Khoury, Anne Auvrignon, Guy Leverger, Georges Audry, Aurore Coulomb, Julie Lemale
No abstract text is available yet for this article.
December 9, 2016: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/27941560/evolution-of-quality-assurance-for-clinical-immunohistochemistry-in-the-era-of-precision-medicine-part-4-tissue-tools-for-quality-assurance-in-immunohistochemistry
#15
Carol C Cheung, Corrado D'Arrigo, Manfred Dietel, Glenn D Francis, Regan Fulton, C Blake Gilks, Jacqueline A Hall, Jason L Hornick, Merdol Ibrahim, Antonio Marchetti, Keith Miller, J Han van Krieken, Soren Nielsen, Paul E Swanson, Clive R Taylor, Mogens Vyberg, Xiaoge Zhou, Emina E Torlakovic
The numbers of diagnostic, prognostic, and predictive immunohistochemistry (IHC) tests are increasing; the implementation and validation of new IHC tests, revalidation of existing tests, as well as the on-going need for daily quality assurance monitoring present significant challenges to clinical laboratories. There is a need for proper quality tools, specifically tissue tools that will enable laboratories to successfully carry out these processes. This paper clarifies, through the lens of laboratory tissue tools, how validation, verification, and revalidation of IHC tests can be performed in order to develop and maintain high quality "fit-for-purpose" IHC testing in the era of precision medicine...
December 9, 2016: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/27941559/expression-of-cas-cse1l-the-cellular-apoptosis-susceptibility-protein-correlates-with-neoplastic-progression-in-barrett-s-esophagus
#16
Kun Jiang, Kevin Neill, Daniel Cowden, Jason Klapman, Steven Eschrich, José Pimiento, Mokenge P Malafa, Domenico Coppola
BACKGROUND: Identifying the molecular switch responsible for the neoplastic progression of Barrett's esophagus (BE) and initiation of adenocarcinoma (ADC) is clinically essential and it will have a profound impact on patient diagnosis, prognosis, and treatment. The cellular apoptosis susceptibility gene CAS/CSE1L is overexpressed in various cancers, including a rare report on esophageal ADC; however, its expression in BE neoplasia has not been addressed. MATERIALS AND METHODS: We investigated the expression of the CAS/CSE1L protein immunohistochemically in 56 esophageal resection specimens for ADC arising in BE...
December 9, 2016: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/27941558/increased-immunoglobulin-g4-positive-plasma-cells-in-lymphadenoma-of-the-salivary-gland-an-immunohistochemical-comparison-among-lymphoepithelial-lesions
#17
Jiyoon Kim, Joon Seon Song, Jong-Lyel Roh, Seung-Ho Choi, Soon Yuhl Nam, Sang Yoon Kim, Kyung-Ja Cho
BACKGROUND: Lymphadenoma (LA) of the salivary gland, nonsebaceous type, is an uncommon benign lymphoepithelial neoplasm. The histogenesis of the lymphoid component of LA is under debate as in Warthin's tumor. A recent study has described immunoglobulin (Ig) class switching to IgG4 in a subset of Warthin's tumors. The aim of this study is to evaluate IgG4 status of LA and presume the role of IgG4 status in pathogenesis of LA. MATERIALS AND METHODS: The distribution of IgG-positive and IgG4-positive cells was compared by immunohistochemistry among 15 lymphoepithelial lesions of the salivary gland (8 LAs, 3 lymphoepithelial carcinomas, and 4 lymphoepithelial cysts)...
December 9, 2016: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/27941557/performance-of-4-immunohistochemical-phosphohistone-h3-antibodies-for-marking-mitotic-figures-in-breast-cancer
#18
Cornelia M Focke, Kai Finsterbusch, Thomas Decker, Paul J van Diest
BACKGROUND: Phosphohistone H3 (PHH3) has been suggested to facilitate and improve mitotic activity assessment in breast cancer and other tumor entities, but the reliability of respective immunohistochemical antibodies has not yet been compared for routine purposes. Our aim was to test the performance of 4 different PHH3 antibodies on a series of highly proliferating breast cancers with good preservation of morphology. METHODS: Four commercially available PHH3 antibodies were tested on 9 grade 3 invasive breast cancers processed in the same batch...
December 9, 2016: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/27801737/digital-image-analysis-of-her2-immunostained-gastric-and-gastroesophageal-junction-adenocarcinomas
#19
Sofie L Nielsen, Søren Nielsen, Mogens Vyberg
Manual assessment of human epidermal growth factor receptor 2 (HER2) protein expression by immunohistochemistry (IHC) in gastric and gastroesophageal junction (GGEJ) adenocarcinomas is prone to interobserver variability and hampered by tumor heterogeneity and different scoring criteria. Equivocal cases are frequent, requiring additional in situ hybridization analysis. This study aimed to evaluate the accuracy of digital image analysis for the assessment of HER2 protein expression. In total, 110 GGEJ adenocarcinomas were included in tissue microarrays with 3 tissue cores per case...
October 31, 2016: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/27801736/an-ios-app-to-expedite-the-evaluation-of-immunohistochemistry-control-tissue
#20
Wilfrido D Mojica, Gregg W Mojica
Control tissues play a vital role in diagnostic immunohistochemistry. They serve to document that the appropriate antibody was used, on the correct control tissue, and run on optimized conditions. As part of the evolving process of standardization in diagnostic immunohistochemistry, specific tissues have been identified based on agreement by experts in this field capable of serving as the benchmark(s) for several antibodies. These tissues are recommended based on known and predictable levels of strong, weak, and no expression of the antigen being queried...
October 31, 2016: Applied Immunohistochemistry & Molecular Morphology: AIMM
journal
journal
34692
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"