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Applied Immunohistochemistry & Molecular Morphology: AIMM

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https://www.readbyqxmd.com/read/29135534/the-multiple-faces-of-programmed-cell-death-ligand-1-expression-in-malignant-and-nonmalignant-cells
#1
Edwin R Parra, Pamela Villalobos, Jaime Rodriguez-Canales
Preliminary data suggest that tumor expression of programmed cell death ligand 1 (PD-L1) protein in human cancers, as determined by immunohistochemistry in formalin-fixed, paraffin-embedded tissue samples, may predict clinical response to anti-PD-1/PD-L1 therapy. PD-L1 is not a specific tumor marker and its expression is also observed in various nonmalignant cells, such as macrophages and lymphocytes, causing confusion in immunohistochemistry analysis when these inflammatory cells are overlapping with tumors cells...
November 13, 2017: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/29084061/tissue-thickness-effects-on-immunohistochemical-staining-intensity-of-markers-of-cancer
#2
Adrienne S McCampbell, Varun Raghunathan, May Tom-Moy, Richard K Workman, Rick Haven, Amir Ben-Dor, Ole F Rasmussen, Lars Jacobsen, Martin Lindberg, N Alice Yamada, Carol Schembri
High-quality patient samples are required for reliable immunohistochemistry test outcomes that provide a significant benefit for patient care. Among the preanalytic variables in tissue handling, tissue thickness is thought to be easily controlled; however, whether the thickness of the tissue effects the staining intensity for antibody immunohistochemistry has not been quantitatively demonstrated. To investigate, we cut multiblock tissue sections of tonsil, liver, and kidney at 2, 4, 6, and 8 μm thicknesses...
October 27, 2017: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/29084060/characterization-of-pd-l1-immunohistochemical-expression-in-cell-blocks-with-different-specimen-fixation-and-processing-methods
#3
Isaac E Lloyd, Wenhua Zhou, Benjamin L Witt, Barbara E Chadwick
Interpretative criteria for programmed death-ligand 1 (PD-L1) immunohistochemistry (IHC) have been largely based on data from formalin-fixed, paraffin-embedded tissues, despite the fact that cytologic specimens, especially cell blocks, are often the only or most readily available tissue for testing. Unlike biopsy specimens, however, cytology sample processing methods can vary markedly. The purpose of this study was to evaluate the effects of several common preanalytic variables on PD-L1 IHC. Two cell lines with strong expression of PD-L1 (H441) and no expression (MCF7) were cultured in vitro...
October 27, 2017: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/29084059/insl3-expression-in-leydig-cell-hyperplasia-and-leydig-cell-tumors
#4
Nelli S Lakis, Kara A Lombardo, Shamlal Mangray, George J Netto, Daniela Salles, Andres Matoso
Insulin-like 3 (INSL3) is a hormone produced by Leydig cells (LCs) and leads to physiological testicular descent during embryonic development. We investigated the expression of INSL3 by immunohistochemistry in normal LCs, in Leydig cell tumor (LCT) (n=17 including 15 testes and 2 ovaries) and in Leydig cell hyperplasia (LCH) (n=10). Normally distributed LCs showed strong immunostaining in the cytoplasm in all cases. All 10 cases (100%) of LCH were strongly and diffusely positive in the intertubular areas. Six cases of LCH had nodules raging in size from 0...
October 27, 2017: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/29084058/renal-cell-carcinoma-with-leiomyomatous-stroma-a-group-of-tumors-with-indistinguishable-histopathologic-features-but-2-distinct-genetic-profiles-next-generation-sequencing-analysis-of-6-cases-negative-for-aberrations-related-to-the-vhl-gene
#5
Fredrik Petersson, Petr Martinek, Tomas Vanecek, Kristyna Pivovarcikova, Kvetoslava Peckova, Ondrej Ondic, Delia Perez-Montiel, Faruk Skenderi, Monika Ulamec, Rudolf Nenutil, Milan Hora, Tomas Svoboda, Pavla Rotterova, Martin Dusek, Michal Michal, Ondrej Hes
We have studied a cohort of renal cell carcinomas (RCCs) with smooth-muscle stroma (N=6), which lacked any of following genetic aberrations: mutations in the VHL-gene-coding sequence, loss of heterozygosity of chromosome 3p, or hypermethylation of VHL. Using targeted next-generation sequencing, no intronic VHL mutations or mutations in selected genes involved in angiogenesis and genes frequently mutated in clear cell RCC were identified. Tumors were also tested for the presence of hotspot mutations in the TCEB1 gene with negative results in all cases...
October 27, 2017: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/29084057/evaluation-of-pd1-pdl1-expression-and-their-clinicopathologic-association-in-ebv-associated-lymphoproliferative-disorders-in-nonimmunosuppressed-patients
#6
Ling Guo, Juraj Bodo, Lisa Durkin, Eric D Hsi
Epstein-Barr virus (EBV)-associated lymphoproliferative disorders (LPD)/lymphomas in nonimmunosuppressed patients represent a unique entity and have been proposed to be related to immune senescence. Engagement of programmed cell death 1 (PD1) by its ligand programmed death ligand 1 (PDL1) inhibits T-cell activation, and leads to T-cell exhaustion. In clinical trials, therapeutic antibodies that block the PD1-PDL1 axis have shown promising therapeutic activity in certain types of lymphomas. Although PD1/PDL1 has been extensively studied in variety of lymphomas, there are few reports characterizing their expression in EBV-positive LPD...
October 27, 2017: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/29084056/are-comprehensive-next-generation-sequencing-analyses-really-required-for-the-management-of-patients-with-melanoma-to-date
#7
Arnaud Uguen
No abstract text is available yet for this article.
October 27, 2017: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/29084055/satb2-and-tle1-expression-in-bcor-ccnb3-ewing-like-sarcoma-mimicking-small-cell-osteosarcoma-and-poorly-differentiated-synovial-sarcoma
#8
David Creytens
No abstract text is available yet for this article.
October 27, 2017: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/29084054/columnar-cell-lesion-and-apocrine-hyperplasia-of-the-breast-is-there-a-common-origin-the-role-of-prolactin-induced-protein
#9
Amedeo Sciarra, Gianluca Lopez, Chiara Corti, Letterio Runza, Giulia Ercoli, Arturo Bonometti, Luca Despini, Concetta Blundo, Donatella Gambini, Nicola Fusco
Noninvasive breast lesions encompass a heterogeneous group of risk indicators and nonobligate precursors of breast cancer, such as apocrine hyperplasia (AH) and columnar cell lesions (CCLs). Given the different expression of ER and ER-regulated genes in AH and CCL, these two alterations are currently considered discrete conditions. However, whether they share early biologic changes is not clear to date. Here, we sought to define the clinicopathologic and immunohistochemical features of a prospective series of combined lesions made up by CCLs and AH forming a continuum within single terminal duct-lobular units...
October 27, 2017: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/29084053/diffuse-s-100-positivity-in-a-meningioma-mimicking-schwannoma-a-diagnostic-pitfall
#10
Mohammed Alsomali, Obiajulu H Iwenofu
No abstract text is available yet for this article.
October 27, 2017: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/29084052/personalizing-gastric-cancer-screening-with-predictive-modeling-of-disease-progression-biomarkers
#11
Rachel Walker, Jaime Mejia, Jae K Lee, Jose M Pimiento, Mokenge Malafa, Anna R Giuliano, Domenico Coppola, Heiko Enderling
Gastric cancer (GC) remains the third most common cause of cancer-related death worldwide. Infection with Helicobacter pylori is responsible for over 70% of GC incidence; colonization induces chronic inflammation, which can facilitate progression to intestinal metaplasia, dysplasia, and GC (Correa pathway). Although H. pylori eradication is a necessary first step in GC prevention, some patients continue to progress to advanced stage disease if substantial tissue damage has occurred or inflammation persists...
October 27, 2017: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/28968271/are-androgen-and-estrogen-receptors-in-dcis-patients-prognostic-indicators-of-relapse-independently-of-treatment
#12
Sara Ravaioli, Maurizio Puccetti, Maria Maddalena Tumedei, Rosella Silvestrini, Lucia Bedei, Sara Bravaccini
Ductal carcinoma in situ (DCIS) is a highly heterogenous tumor that is now more frequently diagnosed because of the increased number of screening programs. Women with DCIS are mainly treated with conservative surgery almost always followed by radiotherapy. Although conventional biomarkers, i.e. ER, PgR, Ki67, and HER2, have been extensively investigated in invasive tumors, little is known about their role in DCIS, especially that of the androgen receptor (AR). In the present study, the expression of conventional biomarkers and AR was determined by immunohistochemistry in 85 DCIS samples from patients monitored for a maximum of 13 years: 43 patients were treated with quadrantectomy and 42 patients underwent quadrantectomy and radiotherapy...
September 29, 2017: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/28968269/immunohistochemical-characterization-of-cancer-associated-fibroblasts-in-oral-squamous-cell-carcinoma
#13
Raísa C Dourado, Lia P A Porto, Águida C G H Leitão, Paloma S G Cerqueira, Jean N Dos Santos, Luciana M P Ramalho, Flávia C A Xavier
Myofibroblasts are differentiated contractile cells that can secrete extracellular matrix components, cytokines, proteases, and proangiogenic factors. In neoplastic processes such as oral squamous cell carcinoma (OSCC), myofibroblasts are recognized as cancer-associated fibroblasts (CAFs) and actively participate in tumor progression. As the presence of myofibroblasts in the stroma may be an important parameter of invasion and proliferation, the aim of this study was to evaluate the presence of CAFs in OSCC by immunophenotyping and their association with histologic classification and clinicopathologic parameters...
September 29, 2017: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/28968268/focal-anomalous-expression-of-cytokeratin-and-p63-in-malignant-phyllodes-tumor-a-comparison-with-spindle-cell-metaplastic-carcinoma
#14
Meenakshi Bansal, Jianzhi Chen, Xi Wang
Differentiating between malignant phyllodes tumors and metaplastic spindle cell carcinomas could be problematic, especially on core biopsies. Immunohistochemical staining for cytokeratin cocktail and p63 has been utilized to differentiate between these tumor types. Forty-three phyllodes tumors (27 benign, 6 borderline, and 10 malignant) and 22 metaplastic carcinomas, consisting at least 80% of spindle cells, were identified. At least 4 tissue blocks from each phyllodes tumor were subjected to immunohistochemical staining for cytokeratin cocktail and p63...
September 29, 2017: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/28968267/evaluation-of-intratumoral-and-intertumoral-heterogeneity-of-met-protein-expression-in-gastric-cancer
#15
Jiwoon Choi, Hee Eun Lee, Hye Seung Lee, Nayoung Han, Min A Kim, Woo Ho Kim
Tumor heterogeneity of a target molecule could contribute to failure of the targeted therapy. We investigated the heterogeneity of MET expression within same primary gastric cancer (GC) and between primary and corresponding secondary GC lesions using immunohistochemistry (IHC). Intratumoral heterogeneity was defined as discordant MET status among 3 tissue microarray cores (3 different areas of same tumor). IHC 3+ was considered positive for MET overexpression. MET overexpression was observed in 2.7% (50/1869) of all examined cores and 5...
September 29, 2017: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/28968266/utility-of-erg-immunohistochemistry-for-evaluation-of-lymphovascular-invasion-in-testicular-germ-cell-tumors-a-retrospective-pilot-study
#16
Aaron M Udager, Jonathan B McHugh, Todd M Morgan, Daniel E Spratt, Arul M Chinnaiyan, Rohit Mehra
Lymphovascular invasion (LVI) of testicular germ cell tumors (GCT) is an important stage-determining variable in the evaluation of radical orchiectomy specimens. ERG endothelial cell expression, as detected by immunohistochemistry (IHC), robustly highlights lymphovascular spaces, and thus, we sought to assess the utility of ERG IHC for evaluation of GCT LVI. Hematoxylin and eosin (H&E) slides from a retrospective cohort of 25 GCT radical orchiectomy specimens (emanating from a parent cohort of 159 radical orchiectomy GCT cases identified between 2003 and 2013) were reviewed, and sections with foci of positive or equivocal LVI were identified...
September 29, 2017: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/28968265/diagnostic-utility-of-pd-l1-expression-in-lung-adenocarcinoma-immunohistochemistry-and-rna-in-situ-hybridization
#17
Mohamad Mazen Gafeer, Kareem Hosny Mohammed, Claudia Ormenisan-Gherasim, Fahad Choudhary, Momin T Siddiqui, Cynthia Cohen
BACKGROUND: Programmed death receptor and programmed death ligand (PD-L1) are immunoregulatory proteins. Nonsmall cell lung cancer bypasses the immune system through the induction of protumorigenic immunosuppressive changes. The better understanding of immunology and antitumor immune responses has brought the promising development of novel immunotherapy agents like programmed death receptor checkpoint inhibitors. The aim of this study was to investigate the expression of PD-L1 in lung adenocarcinoma (ADC), comparing 2 different technologies: immunohistochemistry (IHC) by 2 methods versus RNA in situ hybridization (RISH)...
September 29, 2017: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/28968264/immature-terminal-deoxynucleotidyl-transferase-positive-b-cells-are-detected-in-a-subset-of-adult-and-pediatric-liver-biopsies
#18
Kwun Wah Wen, Ryan M Gill
Terminal deoxynucleotidyl transferase (TdT) is a nuclear enzyme restricted to precursor lymphoid cells and their malignant counterparts; immunohistochemical TdT labeling is helpful in recognition of lymphoblasts, which can resemble mature lymphocytes. The diagnosis of B-lymphoblastic leukemia/lymphoma (B-ALL) is occasionally first encountered on liver core biopsy, but TdT immunostain specificity for B-ALL is not clearly established in this setting, which can be problematic when only a few TdT-positive cells are identified...
September 29, 2017: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/28968263/malignant-ectomesenchymoma-a-potential-pitfall-of-diagnosis-in-the-spectrum-of-pediatric-small-blue-round-cell-tumors
#19
Sabah Boudjemaa, Arnaud Petit
No abstract text is available yet for this article.
September 29, 2017: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/28968262/optical-biomarker-based-biosensors-for-cancer-infectious-disease-medical-diagnoses
#20
Alireza Gharatape, Ahmad Yari Khosroushahi
Thousands of billions of dollars have been disbursed worldwide for the diagnosis and therapy of disease. Nanobioscience combined with sensing technology has facilitated the pathway to diagnosis by introducing a novel, highly sensitive and selective detection system known as the nanobiosensor to reduce the overall cost of treatment. Among the many types of biosensors, optical ones are more easily developed because of their low cost, rapid construction, small size, high performance, high sensitivity and selectivity, high signal-to-noise ratio, flexible geometry, and portability properties...
September 29, 2017: Applied Immunohistochemistry & Molecular Morphology: AIMM
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