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Journal of Biomolecular Techniques: JBT

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https://www.readbyqxmd.com/read/28785175/evaluation-of-a-bead-free-coimmunoprecipitation-technique-for-identification-of-virus-host-protein-interactions-using-high-resolution-mass-spectrometry
#1
Stacy L DeBlasio, Michael S Bereman, Jaclyn Mahoney, Theodore W Thannhauser, Stewart M Gray, Michael J MacCoss, Michelle Cilia Heck
Protein interactions between virus and host are essential for viral propagation and movement, as viruses lack most of the proteins required to thrive on their own. Precision methods aimed at disrupting virus-host interactions represent new approaches to disease management but require in-depth knowledge of the identity and binding specificity of host proteins within these interaction networks. Protein coimmunoprecipitation (co-IP) coupled with mass spectrometry (MS) provides a high-throughput way to characterize virus-host interactomes in a single experiment...
July 24, 2017: Journal of Biomolecular Techniques: JBT
https://www.readbyqxmd.com/read/28860948/outstanding-scientific-poster-awards-for-the-abrf-2017-organized-by-the-education-committee-and-sponsored-by-waters-corporation
#2
Ron Orlando
No abstract text is available yet for this article.
September 2017: Journal of Biomolecular Techniques: JBT
https://www.readbyqxmd.com/read/28860947/article-watch-september-2017
#3
Clive A Slaughter
This column highlights recently published articles that are of interest to the readership of this publication. We encourage ABRF members to forward information on articles they feel are important and useful to Clive Slaughter, MCG-UGA Medical Partnership, 1425 Prince Ave., Athens GA 30606, USA. Tel: (706) 713-2216; Fax: (706) 713-2221; E-mail; cslaught@uga.edu, or to any member of the editorial board. Article summaries reflect the reviewer's opinions and not necessarily those of the association.
September 2017: Journal of Biomolecular Techniques: JBT
https://www.readbyqxmd.com/read/28785176/predicting-the-retention-behavior-of-specific-o-linked-glycopeptides
#4
Majors J Badgett, Barry Boyes, Ron Orlando
O-Linked glycosylation is a common post-translational modification that can alter the overall structure, polarity, and function of proteins. Reverse-phase (RP) chromatography is the most common chromatographic approach to analyze O-glycosylated peptides and their unmodified counterparts, even though this approach often does not provide adequate separation of these two species. Hydrophilic interaction liquid chromatography (HILIC) can be a solution to this problem, as the polar glycan interacts with the polar stationary phase and potentially offers the ability to resolve the peptide from its modified form(s)...
September 2017: Journal of Biomolecular Techniques: JBT
https://www.readbyqxmd.com/read/28630600/-unknown-title
#5
(no author information available yet)
[This corrects the article on p. 46 in vol. 28, PMID: 28344519.].
July 2017: Journal of Biomolecular Techniques: JBT
https://www.readbyqxmd.com/read/28630599/-unknown-title
#6
(no author information available yet)
[This corrects the article on p. 40 in vol. 28, PMID: 28337072.].
July 2017: Journal of Biomolecular Techniques: JBT
https://www.readbyqxmd.com/read/28630598/-unknown-title
#7
(no author information available yet)
[This corrects the article on p. 8 in vol. 28, PMID: 28337071.].
July 2017: Journal of Biomolecular Techniques: JBT
https://www.readbyqxmd.com/read/28630597/-unknown-title
#8
(no author information available yet)
[This corrects the article on p. 31 in vol. 28, PMID: 28337070.].
July 2017: Journal of Biomolecular Techniques: JBT
https://www.readbyqxmd.com/read/28507462/comparative-analysis-of-non-viral-transfection-methods-in-mouse-embryonic-fibroblast-cells
#9
Migi Lee, Kathleen Chea, Rajyalakshmi Pyda, Melissa Chua, Isabel Dominguez
Mouse embryonic fibroblast (MEF) cells are an important in vitro model for developmental biology, disease, and reprogramming studies. However, as with other primary cells, they are challenging to transfect. Although viral gene-delivery methods achieve high gene-delivery efficiency, challenges with cell mutagenesis and safety among others have led to the use and improvement of non-viral gene-delivery methods in MEF cells. Despite the importance of gene delivery in MEF cells, there is limited comparison of method/reagent efficacy...
July 2017: Journal of Biomolecular Techniques: JBT
https://www.readbyqxmd.com/read/28058039/assessment-of-il-28-rs12979860-and-rs8099917-polymorphisms-in-a-cohort-of-cuban-chronic-hcv-genotype-1b-patients
#10
Daniel Palenzuela Gardón, Isabel Alicia Guillen, Julio R Fernández, Hamlet Camacho, Zurina Cinza Estevez, Santiago Dueñas, Liz Alvares-Lajonchere, Yalena Amador, Gillian Martinez-Donato, Junsong Han, Zhiming Zhang, Xiaona Zhang, Yang Gao, Juan Roca Campaña, Lidia I Novoa
Hepatitis C virus (HCV) is a significant global public health problem with >185 million infections worldwide. A series of genome-wide association studies (GWAS) has identified IL-28B polymorphisms as a predictor of sustained virologic response (SVR), as well as spontaneous clearance in chronic HCV genotype 1 patients. The objective of this work was to evaluate the prevalence of IL-28B rs12979860 and rs8099917 polymorphisms in Cuban chronic HCV patients. The study cohort included 73 chronic HCV patients treated with concomitant administration of CIGB-230 and nonpegylated IFN-α plus ribavirin (non-pegIFN-α/R) antiviral therapy...
July 2017: Journal of Biomolecular Techniques: JBT
https://www.readbyqxmd.com/read/28785174/-squirrel-primer-based-pcr-assay-for-direct-and-targeted-sanger-sequencing-of-short-genomic-segments
#11
Henry O Ebili, James C Hassall, Wakkas Fadhil, Hersh Ham-Karim, Abutaleb Asiri, Teresa P Raposo, Ayodeji Johnson Agboola, Mohammad Ilyas
Currently, short DNA segments of sub-100 bp can be sequenced either directly by next-generation sequencing and pyrosequencing, which are expensive, or indirectly, via Sanger sequencing combined with the cumbersome and failure-prone plasmid cloning. To circumvent these issues, we have generated a novel sequencing-purposed PCR assay using long-tailed primers (squirrel primers) to Sanger sequence directly sub-100 bp genomic amplicons. Squirrel primers, 40-65 nt in length, were used to amplify 51-93 bp long genomic sequences of KRAS exons 2 and 3, BRAF exon 15, PI3K catalytic subunit alpha exon 20, and phosphatase and tensin homolog exon 3 from colorectal cancer (CRC) cell lines and preamplified clinical CRC samples with known mutation status by PCR...
June 6, 2017: Journal of Biomolecular Techniques: JBT
https://www.readbyqxmd.com/read/28584520/article-watch-july-2017
#12
Clive A Slaughter
This column highlights recently published articles that are of interest to the readership of this publication. We encourage ABRF members to forward information on articles they feel are important and useful to Clive Slaughter, MCG-UGA Medical Partnership, 1425 Prince Ave., Athens, GA 30606, USA. Tel: (706) 713-2216; Fax: (706) 713-2221; E-mail: cslaught@uga.edu, or to any member of the editorial board. Article summaries reflect the reviewer's opinions and not necessarily those of the association.
May 25, 2017: Journal of Biomolecular Techniques: JBT
https://www.readbyqxmd.com/read/28507461/washed-away-how-not-to-lose-your-rna-during-isolation
#13
Birgitte K Hønsvall, Lucy J Robertson
Cryptosporidium oocysts have extremely robust walls that protect the parasite against environmental pressures. Analyses must be sensitive to detect the few organisms (if any) present in environmental samples. After a series of negative nucleic acid amplification results on spiked samples, following a standard RNA isolation protocol, it seemed probable that oocyst RNA had been lost in the washing steps of the isolation protocol. By reducing both the volume of wash buffer and the number of washing steps, positive results could be re-established...
April 28, 2017: Journal of Biomolecular Techniques: JBT
https://www.readbyqxmd.com/read/28400709/focus-on-metagenomics
#14
Christopher E Mason, Scott Tighe
No abstract text is available yet for this article.
April 2017: Journal of Biomolecular Techniques: JBT
https://www.readbyqxmd.com/read/28344519/assessment-of-repli-g-multiple-displacement-whole-genome-amplification-wga-techniques-for-metagenomic-applications
#15
Sofia Ahsanuddin, Ebrahim Afshinnekoo, Jorge Gandara, Mustafa Hakyemezoğlu, Daniela Bezdan, Samuel Minot, Nick Greenfield, Christopher E Mason
Amplification of minute quantities of DNA is a fundamental challenge in low-biomass metagenomic and microbiome studies because of potential biases in coverage, guanine-cytosine (GC) content, and altered species abundances. Whole genome amplification (WGA), although widely used, is notorious for introducing artifact sequences, either by amplifying laboratory contaminants or by nonrandom amplification of a sample's DNA. In this study, we investigate the effect of REPLI-g multiple displacement amplification (MDA; Qiagen, Valencia, CA, USA) on sequencing data quality and species abundance detection in 8 paired metagenomic samples and 1 titrated, mixed control sample...
April 2017: Journal of Biomolecular Techniques: JBT
https://www.readbyqxmd.com/read/28337073/real-time-dna-sequencing-in-the-antarctic-dry-valleys-using-the-oxford-nanopore-sequencer
#16
Sarah S Johnson, Elena Zaikova, David S Goerlitz, Yu Bai, Scott W Tighe
The ability to sequence DNA outside of the laboratory setting has enabled novel research questions to be addressed in the field in diverse areas, ranging from environmental microbiology to viral epidemics. Here, we demonstrate the application of offline DNA sequencing of environmental samples using a hand-held nanopore sequencer in a remote field location: the McMurdo Dry Valleys, Antarctica. Sequencing was performed using a MK1B MinION sequencer from Oxford Nanopore Technologies (ONT; Oxford, United Kingdom) that was equipped with software to operate without internet connectivity...
April 2017: Journal of Biomolecular Techniques: JBT
https://www.readbyqxmd.com/read/28337072/precision-metagenomics-rapid-metagenomic-analyses-for-infectious-disease-diagnostics-and-public-health-surveillance
#17
Ebrahim Afshinnekoo, Chou Chou, Noah Alexander, Sofia Ahsanuddin, Audrey N Schuetz, Christopher E Mason
Next-generation sequencing (NGS) technologies have ushered in the era of precision medicine, transforming the way we treat cancer patients and diagnose disease. Concomitantly, the advent of these technologies has created a surge of microbiome and metagenomic studies over the last decade, many of which are focused on investigating the host-gene-microbial interactions responsible for the development and spread of infectious diseases, as well as delineating their key role in maintaining health. As we continue to discover more information about the etiology of infectious diseases, the translational potential of metagenomic NGS methods for treatment and rapid diagnosis is becoming abundantly clear...
April 2017: Journal of Biomolecular Techniques: JBT
https://www.readbyqxmd.com/read/28337071/international-standards-for-genomes-transcriptomes-and-metagenomes
#18
REVIEW
Christopher E Mason, Ebrahim Afshinnekoo, Scott Tighe, Shixiu Wu, Shawn Levy
Challenges and biases in preparing, characterizing, and sequencing DNA and RNA can have significant impacts on research in genomics across all kingdoms of life, including experiments in single-cells, RNA profiling, and metagenomics (across multiple genomes). Technical artifacts and contamination can arise at each point of sample manipulation, extraction, sequencing, and analysis. Thus, the measurement and benchmarking of these potential sources of error are of paramount importance as next-generation sequencing (NGS) projects become more global and ubiquitous...
April 2017: Journal of Biomolecular Techniques: JBT
https://www.readbyqxmd.com/read/28337070/genomic-methods-and-microbiological-technologies-for-profiling-novel-and-extreme-environments-for-the-extreme-microbiome-project-xmp
#19
Scott Tighe, Ebrahim Afshinnekoo, Tara M Rock, Ken McGrath, Noah Alexander, Alexa McIntyre, Sofia Ahsanuddin, Daniela Bezdan, Stefan J Green, Samantha Joye, Sarah Stewart Johnson, Don A Baldwin, Nathan Bivens, Nadim Ajami, Joseph R Carmical, Ian Charold Herriott, Rita Colwell, Mohamed Donia, Jonathan Foox, Nick Greenfield, Tim Hunter, Jessica Hoffman, Joshua Hyman, Ellen Jorgensen, Diana Krawczyk, Jodie Lee, Shawn Levy, Natàlia Garcia-Reyero, Matthew Settles, Kelley Thomas, Felipe Gómez, Lynn Schriml, Nikos Kyrpides, Elena Zaikova, Jon Penterman, Christopher E Mason
The Extreme Microbiome Project (XMP) is a project launched by the Association of Biomolecular Resource Facilities Metagenomics Research Group (ABRF MGRG) that focuses on whole genome shotgun sequencing of extreme and unique environments using a wide variety of biomolecular techniques. The goals are multifaceted, including development and refinement of new techniques for the following: 1) the detection and characterization of novel microbes, 2) the evaluation of nucleic acid techniques for extremophilic samples, and 3) the identification and implementation of the appropriate bioinformatics pipelines...
April 2017: Journal of Biomolecular Techniques: JBT
https://www.readbyqxmd.com/read/28260999/gut-microbiota-analysis-results-are-highly-dependent-on-the-16s-rrna-gene-target-region-whereas-the-impact-of-dna-extraction-is-minor
#20
Anniina Rintala, Sami Pietilä, Eveliina Munukka, Erkki Eerola, Juha-Pekka Pursiheimo, Asta Laiho, Satu Pekkala, Pentti Huovinen
Next-generation sequencing (NGS) is currently the method of choice for analyzing gut microbiota composition. As gut microbiota composition is a potential future target for clinical diagnostics, it is of utmost importance to enhance and optimize the NGS analysis procedures. Here, we have analyzed the impact of DNA extraction and selected 16S rDNA primers on the gut microbiota NGS results. Bacterial DNA from frozen stool specimens was extracted with 5 commercially available DNA extraction kits. Special attention was paid to the semiautomated DNA extraction methods that could expedite the analysis procedure, thus being especially suitable for clinical settings...
April 2017: Journal of Biomolecular Techniques: JBT
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