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Journal of Biomolecular Techniques: JBT

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https://www.readbyqxmd.com/read/29623006/development-of-a-membrane-based-method-for-isolation-of-genomic-dna-from-human-blood
#1
Goutam Mandal, Subhasish Das, Sriram Padmanabhan
High quality and sufficient quantity of genomic DNA (gDNA) are the primary requisites of several molecular biologic applications, including clinical studies related to genetics, genomics, gene polymorphism, and DNA fingerprinting. Whole blood is the primary source of gDNA in most of the clinical investigations. Currently, commercial kits are primarily used to achieve these goals. However, the use of kits is limited by the cost and involvement of several centrifugal steps. Other methods reported are either laborious or do not produce high quality or quantity of gDNA or both...
March 23, 2018: Journal of Biomolecular Techniques: JBT
https://www.readbyqxmd.com/read/29623005/challenges-and-opportunities-for-biological-mass-spectrometry-core-facilities-in-the-developing-world
#2
Liam Bell, Bridget Calder, Reinhard Hiller, Ashwil Klein, Nelson C Soares, Stoyan H Stoychev, Barend C Vorster, David L Tabb
The developing world is seeing rapid growth in the availability of biological mass spectrometry (MS), particularly through core facilities. As proteomics and metabolomics becomes locally feasible for investigators in these nations, application areas associated with high burden in these nations, such as infectious disease, will see greatly increased research output. This article evaluates the rapid growth of MS in South Africa (currently approaching 20 laboratories) as a model for establishing MS core facilities in other nations of the developing world...
March 23, 2018: Journal of Biomolecular Techniques: JBT
https://www.readbyqxmd.com/read/29618955/content-is-king-databases-preserve-the-collective-information-of-science
#3
John R Yates
Databases store sequence information experimentally gathered to create resources that further science. In the last 20 years databases have become critical components of fields like proteomics where they provide the basis for large-scale and high-throughput proteomic informatics. Amos Bairoch, winner of the Association of Biomolecular Resource Facilities Frederick Sanger Award, has created some of the important databases proteomic research depends upon for accurate interpretation of data.
March 22, 2018: Journal of Biomolecular Techniques: JBT
https://www.readbyqxmd.com/read/29667651/-unknown-title
#4
(no author information available yet)
[This corrects the article on p. 137 in vol. 28, PMID: 29151820.].
April 2018: Journal of Biomolecular Techniques: JBT
https://www.readbyqxmd.com/read/29667650/-unknown-title
#5
(no author information available yet)
[This corrects the article on p. 137 in vol. 28, PMID: 29151820.].
April 2018: Journal of Biomolecular Techniques: JBT
https://www.readbyqxmd.com/read/29463959/article-watch-april-2018
#6
REVIEW
Clive A Slaughter
This column highlights recently published articles that are of interest to the readership of this publication. We encourage ABRF members to forward information on articles they feel are important and useful to Clive Slaughter, MCG-UGA Medical Partnership, 1425 Prince Ave., Athens, GA 30606, USA. Tel: (706) 713-2216; Fax: (706) 713-2221; E-mail: cslaught@uga.edu, or to any member of the editorial board. Article summaries reflect the reviewer's opinions and not necessarily those of the association.
April 2018: Journal of Biomolecular Techniques: JBT
https://www.readbyqxmd.com/read/29151820/name-it-store-it-protect-it-a-systems-approach-to-managing-data-in-research-core-facilities
#7
REVIEW
Matthew DeVries, Matthew Fenchel, R E Fogarty, Byong-Do Kim, Daniel Timmons, A Nicole White
As the capabilities of technology increase, so do the production of data and the need for data management. The need for data storage at many academic institutions is increasing exponentially. Technology is expanding rapidly, and institutions are recognizing the need to incorporate data management that can be available for future data sharing as a critical component of institutional services. The establishment of a process to manage the surge in data storage is complex and often hindered by not having a plan...
December 2017: Journal of Biomolecular Techniques: JBT
https://www.readbyqxmd.com/read/29118677/article-watch-december-2017
#8
REVIEW
Clive A Slaughter
This column highlights recently published articles that are of interest to the readership of this publication. We encourage ABRF members to forward information on articles they feel are important and useful to Clive Slaughter, MCG-UGA Medical Partnership, 1425 Prince Ave., Athens, GA 30606, USA. Tel: (706) 713-2216; Fax: (706) 713-2221; E-mail: cslaught@uga.edu, or to any member of the editorial board. Article summaries reflect the reviewer's opinions and not necessarily those of the association.
December 2017: Journal of Biomolecular Techniques: JBT
https://www.readbyqxmd.com/read/29042829/kinetics-of-n-glycan-release-from-human-immunoglobulin-g-igg-by-pngase-f-all-glycans-are-not-created-equal
#9
Yining Huang, Ron Orlando
The biologic activity of IgG molecules is modulated by its crystallizable fragment N-glycosylation, and thus, the analysis of IgG glycosylation is critical. A standard approach to analyze glycosylation of IgGs involves the release of the N-glycans by the enzyme peptide N-glycosidase F, which cleaves the linkage between the asparagine residue and innermost N-acetylglucosamine (GlcNAc) of all N-glycans except those containing a 3-linked fucose attached to the reducing terminal GlcNAc residue. The importance of obtaining complete glycan release for accurate quantitation led us to investigate the kinetics of this de-glycosylation reaction for IgG glycopeptides and to determine the effect of glycan structure and amino acid sequence on the rate of glycan release from glycopeptides of IgGs...
December 2017: Journal of Biomolecular Techniques: JBT
https://www.readbyqxmd.com/read/29038641/a-method-for-mapping-glycosylation-sites-in-proteins
#10
Mark S Han, John T Simpson
The analysis of protein glycosylation by mass spectrometry (MS) has been a challenging technical problem. Quantification by HPLC of N -linked glycans can be executed by the use of peptide- N -glycosidase F to release them from the protein, followed by attachment of a fluorescent label and subsequent fluorescence detection. Similar quantification of O -linked glycans is not possible, as a result of the lack of a universal deglycosylation enzyme. Site-specific analyses by MS, such as the use of proteases to digest the glycoprotein, are difficult to use for quantification of glycans, as a result of the presence of miscleavages...
December 2017: Journal of Biomolecular Techniques: JBT
https://www.readbyqxmd.com/read/28860948/outstanding-scientific-poster-awards-for-the-abrf-2017-organized-by-the-education-committee-and-sponsored-by-waters-corporation
#11
Ron Orlando
No abstract text is available yet for this article.
September 2017: Journal of Biomolecular Techniques: JBT
https://www.readbyqxmd.com/read/28860947/article-watch-september-2017
#12
Clive A Slaughter
This column highlights recently published articles that are of interest to the readership of this publication. We encourage ABRF members to forward information on articles they feel are important and useful to Clive Slaughter, MCG-UGA Medical Partnership, 1425 Prince Ave., Athens GA 30606, USA. Tel: (706) 713-2216; Fax: (706) 713-2221; E-mail; cslaught@uga.edu, or to any member of the editorial board. Article summaries reflect the reviewer's opinions and not necessarily those of the association.
September 2017: Journal of Biomolecular Techniques: JBT
https://www.readbyqxmd.com/read/28785176/predicting-the-retention-behavior-of-specific-o-linked-glycopeptides
#13
Majors J Badgett, Barry Boyes, Ron Orlando
O-Linked glycosylation is a common post-translational modification that can alter the overall structure, polarity, and function of proteins. Reverse-phase (RP) chromatography is the most common chromatographic approach to analyze O-glycosylated peptides and their unmodified counterparts, even though this approach often does not provide adequate separation of these two species. Hydrophilic interaction liquid chromatography (HILIC) can be a solution to this problem, as the polar glycan interacts with the polar stationary phase and potentially offers the ability to resolve the peptide from its modified form(s)...
September 2017: Journal of Biomolecular Techniques: JBT
https://www.readbyqxmd.com/read/28785175/evaluation-of-a-bead-free-coimmunoprecipitation-technique-for-identification-of-virus-host-protein-interactions-using-high-resolution-mass-spectrometry
#14
Stacy L DeBlasio, Michael S Bereman, Jaclyn Mahoney, Theodore W Thannhauser, Stewart M Gray, Michael J MacCoss, Michelle Cilia Heck
Protein interactions between virus and host are essential for viral propagation and movement, as viruses lack most of the proteins required to thrive on their own. Precision methods aimed at disrupting virus-host interactions represent new approaches to disease management but require in-depth knowledge of the identity and binding specificity of host proteins within these interaction networks. Protein coimmunoprecipitation (co-IP) coupled with mass spectrometry (MS) provides a high-throughput way to characterize virus-host interactomes in a single experiment...
September 2017: Journal of Biomolecular Techniques: JBT
https://www.readbyqxmd.com/read/28630600/-unknown-title
#15
(no author information available yet)
[This corrects the article on p. 46 in vol. 28, PMID: 28344519.].
July 2017: Journal of Biomolecular Techniques: JBT
https://www.readbyqxmd.com/read/28630599/-unknown-title
#16
(no author information available yet)
[This corrects the article on p. 40 in vol. 28, PMID: 28337072.].
July 2017: Journal of Biomolecular Techniques: JBT
https://www.readbyqxmd.com/read/28630598/-unknown-title
#17
(no author information available yet)
[This corrects the article on p. 8 in vol. 28, PMID: 28337071.].
July 2017: Journal of Biomolecular Techniques: JBT
https://www.readbyqxmd.com/read/28630597/-unknown-title
#18
(no author information available yet)
[This corrects the article on p. 31 in vol. 28, PMID: 28337070.].
July 2017: Journal of Biomolecular Techniques: JBT
https://www.readbyqxmd.com/read/28507462/comparative-analysis-of-non-viral-transfection-methods-in-mouse-embryonic-fibroblast-cells
#19
Migi Lee, Kathleen Chea, Rajyalakshmi Pyda, Melissa Chua, Isabel Dominguez
Mouse embryonic fibroblast (MEF) cells are an important in vitro model for developmental biology, disease, and reprogramming studies. However, as with other primary cells, they are challenging to transfect. Although viral gene-delivery methods achieve high gene-delivery efficiency, challenges with cell mutagenesis and safety among others have led to the use and improvement of non-viral gene-delivery methods in MEF cells. Despite the importance of gene delivery in MEF cells, there is limited comparison of method/reagent efficacy...
July 2017: Journal of Biomolecular Techniques: JBT
https://www.readbyqxmd.com/read/28058039/assessment-of-il-28-rs12979860-and-rs8099917-polymorphisms-in-a-cohort-of-cuban-chronic-hcv-genotype-1b-patients
#20
Daniel Palenzuela Gardón, Isabel Alicia Guillen, Julio R Fernández, Hamlet Camacho, Zurina Cinza Estevez, Santiago Dueñas, Liz Alvares-Lajonchere, Yalena Amador, Gillian Martinez-Donato, Junsong Han, Zhiming Zhang, Xiaona Zhang, Yang Gao, Juan Roca Campaña, Lidia I Novoa
Hepatitis C virus (HCV) is a significant global public health problem with >185 million infections worldwide. A series of genome-wide association studies (GWAS) has identified IL-28B polymorphisms as a predictor of sustained virologic response (SVR), as well as spontaneous clearance in chronic HCV genotype 1 patients. The objective of this work was to evaluate the prevalence of IL-28B rs12979860 and rs8099917 polymorphisms in Cuban chronic HCV patients. The study cohort included 73 chronic HCV patients treated with concomitant administration of CIGB-230 and nonpegylated IFN-α plus ribavirin (non-pegIFN-α/R) antiviral therapy...
July 2017: Journal of Biomolecular Techniques: JBT
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