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Biomedical Microdevices

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https://www.readbyqxmd.com/read/29138988/irreversible-electroporation-of-the-uterine-cervix-in-a-rabbit-model
#1
Wei Chai, Wenlong Zhang, Zhentong Wei, Ying Xu, Jian Shi, Xiaomei Luo, Jianying Zeng, Manhua Cui, Jiannan Li, Lizhi Niu
The aim of this study was to evaluate the effects of irreversible electroporation (IRE) on the uterine cervix in a rabbit model. IRE ablation was performed in the cervices of 48 New Zealand rabbits, with one ablation lesion in each animal. Gross pathology, transmission electron microscopy, hematoxylin and eosin (H&E), Masson's trichrome (MT) stain, and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assays were performed at pre-set time points (0 h, 12 h, 1 d, 2 d, 4 d, 7 d, 14 d, and 28 d post-IRE)...
November 14, 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/29134412/engineered-bone-scaffolds-with-dielectrophoresis-based-patterning-using-3d-printing
#2
Zhijie Huan, Henry K Chu, Hongbo Liu, Jie Yang, Dong Sun
Patterning of cells into a specific pattern is an important procedure in tissue engineering to facilitate tissue culture and ingrowth. In this paper, a new type of 3D-printed scaffold utilizing dielectrophoresis (DEP) for active cell seeding and patterning was proposed. This scaffold adopted a concentric-ring design that is similar to native bone tissues. The scaffold was fabricated with a commercial three-dimensional (3D) printer. Polylactic Acid (PLA) was selected as the material for the printer and the fabricated scaffold was coated with gold to enhance the conductivity for DEP manipulation...
November 13, 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/29128921/a-microfluidic-in-line-elisa-for-measuring-secreted-protein-under-perfusion
#3
Qiyue Luan, Stacey Cahoon, Agnes Wu, Shyam Sundhar Bale, Martin Yarmush, Abhinav Bhushan
Recent progress in the development of microfluidic microphysiological systems such as 'organs-on-chips' and microfabricated cell culture is geared to simulate organ-level physiology. These tissue models leverage microengineering technologies that provide capabilities of presenting cultured cells with input signals in a more physiologically relevant context such as perfused flow. Proteins that are secreted from cells have important information about the health of the cells. Techniques to quantify cellular proteins include mass spectrometry to ELISA (enzyme-linked immunosorbent assay)...
November 11, 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/29116458/3d-gut-liver-chip-with-a-pk-model-for-prediction-of-first-pass-metabolism
#4
Dong Wook Lee, Sang Keun Ha, Inwook Choi, Jong Hwan Sung
Accurate prediction of first-pass metabolism is essential for improving the time and cost efficiency of drug development process. Here, we have developed a microfluidic gut-liver co-culture chip that aims to reproduce the first-pass metabolism of oral drugs. This chip consists of two separate layers for gut (Caco-2) and liver (HepG2) cell lines, where cells can be co-cultured in both 2D and 3D forms. Both cell lines were maintained well in the chip, verified by confocal microscopy and measurement of hepatic enzyme activity...
November 7, 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/29116415/a-microfluidic-device-for-quantitative-investigation-of-zebrafish-larvae-s-rheotaxis
#5
Amir Reza Peimani, Georg Zoidl, Pouya Rezai
Zebrafish is a model organism for various sensory-motor biological studies. Rheotaxis, or the ability of zebrafish to orient and swim against the water stream, is a common behavior that involves multiple sensory-motor processes such as their lateral line and visual systems. Due to the lack of a controllable and easy-to-use assay, zebrafish rheotaxis at larval stages is not well-understood. In this paper, we report a microfluidic device that can be used to apply the flow stimulus precisely and repeatedly along the longitudinal axis of individual zebrafish larvae to study their coaxial rheotaxis...
November 7, 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/29116412/fabrication-of-flexible-thin-polyurethane-membrane-for-tissue-engineering-applications
#6
A Arefin, J-H Huang, D Platts, V D Hypes, J F Harris, R Iyer, P Nath
Thin and flexible polymeric membranes play a critical role in tissue engineering applications for example organs-on-a-chip. These flexible membranes can enable mechanical stretch of the engineered tissue to mimic organ-specific biophysical features, such as breathing. In this work, we report the fabrication of thin (<20 μm), stretchable, and biocompatible polyurethane (PU) membranes. The membranes were fabricated using spin coating technique on silicon substrates and were mounted on a frame for ease of device integration and handling...
November 7, 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/29103075/measurement-of-cell-traction-force-with-a-thin-film-pdms-cantilever
#7
Merrel T Holley, Ashkan YekrangSafakar, Mazvita Maziveyi, Suresh K Alahari, Kidong Park
Adherent cells produce cellular traction force (CTF) on a substrate to maintain their physical morphologies, sense external environment, and perform essential cellular functions. Precise characterization of the CTF can expand our knowledge of various cellular processes as well as lead to the development of novel mechanical biomarkers. However, current methods that measure CTF require special substrates and fluorescent microscopy, rendering them less suitable in a clinical setting. Here, we demonstrate a rapid and direct approach to measure the combined CTF of a large cell population using thin polydimethylsiloxane (PDMS) cantilevers...
November 4, 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/29098446/correction-to-polymer-based-interconnection-cables-to-integrate-with-flexible-penetrating-microelectrode-arrays
#8
Keonghwan Oh, Donghak Byun, Sohee Kim
The original version of this article unfortunately contained a mistake. In Eqs. (1) to (3), the gas constant 'R' should be replaced with Boltzmann constant 'k B.' This change accompanies the change in the paragraph following Eq. (3) as below.
November 2, 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/29082438/dielectrophoretic-microbead-sorting-using-modular-electrode-design-and-capillary-driven-microfluidics
#9
Jaione Tirapu-Azpiroz, Yuksel Temiz, Emmanuel Delamarche
Multiplexing assays using microbeads in microfluidics offers high flexibility and throughput, but requires the ability to sort particles based on their physical properties. In this paper, we present a continuous method for separating microbeads that is compact, modular and adaptive, employing an optimized electrode layout that alternates sorting and concentration of microbeads using dielectrophoresis and a nested design. By simulating the combined effects of the hydrodynamic drag and dielectrophoresis forces on polystyrene beads, the parameters of the electrode layout and voltage configuration are optimized for maximum separation based on particle size with a small number of slanted planar electrodes...
October 30, 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/29071494/highly-efficient-isolation-and-release-of-circulating-tumor-cells-based-on-size-dependent-filtration-and-degradable-zno-nanorods-substrate-in-a-wedge-shaped-microfluidic-chip
#10
Songzhan Li, Yifan Gao, Xiran Chen, Luman Qin, Boran Cheng, Shubin Wang, Shengxiang Wang, Guangxin Zhao, Kan Liu, Nangang Zhang
Circulating tumor cells (CTCs) have been regarded as the major cause of metastasis, holding significant insights for tumor diagnosis and treatment. Although many efforts have been made to develop methods for CTC isolation and release in microfluidic system, it remains significant challenges to realize highly efficient isolation and gentle release of CTCs for further cellular and bio-molecular analyses. In this study, we demonstrate a novel method for CTC isolation and release using a simple wedge-shaped microfluidic chip embedding degradable znic oxide nanorods (ZnNRs) substrate...
October 25, 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/29071421/effect-of-drug-loaded-tio2-nanotube-arrays-on-osseointegration-in-an-orthodontic-miniscrew-an-in-vivo-pilot-study
#11
Insan Jang, Dong-Soon Choi, Jae-Kwan Lee, Wan-Tae Kim, Bong-Kuen Cha, Won-Youl Choi
Osseointegration was evaluated for the surface of miniscrews with TiO2 nanotube arrays containing drugs in this in-vivo study. The diameter and length of the TiO2 nanotube arrays were about 70 nm and 5 μm, respectively. Recombinant human bone morphogenetic protein-2 (rhBMP-2) or ibuprofen was loaded in the TiO2 nanotube arrays with 12 miniscrews. The 12 drug-loaded miniscrews, 6 miniscrews with no drug-loaded TiO2 nanotube arrays and 6 conventional miniscrews, were placed on the tibias of New Zealand white rabbits...
October 25, 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/29038872/a-novel-method-to-understand-tumor-cell-invasion-integrating-extracellular-matrix-mimicking-layers-in-microfluidic-chips-by-selective-curing
#12
H Eslami Amirabadi, S SahebAli, J P Frimat, R Luttge, J M J den Toonder
A major challenge in studying tumor cell invasion into its surrounding tissue is to identify the contribution of individual factors in the tumor microenvironment (TME) to the process. One of the important elements of the TME is the fibrous extracellular matrix (ECM) which is known to influence cancer cell invasion, but exactly how remains unclear. Therefore, there is a need for new models to unravel mechanisms behind the tumor-ECM interaction. In this article, we present a new microfabrication method, called selective curing, to integrate ECM-mimicking layers between two microfluidic channels...
October 17, 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28994005/characterisation-of-human-induced-pluripotent-stem-cell-derived-endothelial-cells-under-shear-stress-using-an-easy-to-use-microfluidic-cell-culture-system
#13
Rsituko Ohtani-Kaneko, Kenjiro Sato, Atsuhiro Tsutiya, Yuka Nakagawa, Kazutoshi Hashizume, Hidekatsu Tazawa
Induced pluripotent stem cell-derived endothelial cells (iPSC-ECs) can contribute to elucidating the pathogenesis of heart and vascular diseases and developing their treatments. Their precise characteristics in fluid flow however remain unclear. Therefore, the aim of the present study is to characterise these features. We cultured three types of ECs in a microfluidic culture system: commercially available human iPS-ECs, human umbilical vein endothelial cells (HUVECs) and human umbilical artery endothelial cells (HUAECs)...
October 9, 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28993931/irreversible-electroporation-in-the-eradication-of-rabbit-vx2-cervical-tumors
#14
Wei Chai, Ying Xu, Wenlong Zhang, Zhentong Wei, Jiannan Li, Jian Shi, Xiaomei Luo, Jianying Zeng, Manhua Cui, Lizhi Niu
The aim of this study was to evaluate the effects of irreversible electroporation (IRE) on the eradication of rabbit VX2 cervical tumors. A VX2 cervical cancer model was first made in 20 New Zealand rabbits. IRE ablation was performed for the cervical cancers of 15 rabbits when the diameter of the tumor was about 1.0-1.5 cm. The control group (n = 5) did not receive IRE ablation. The gross pathology, ultrasound, computed tomography, hematoxylin and eosin, terminal deoxynucleotidyl transferase dUTP nick end labeling assay, and proliferating cell nuclear antigen immunohistochemical staining were performed to evaluate the efficacy of IRE on cervical cancer...
October 9, 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28965149/microfluidic-device-for-novel-breast-cancer-screening-by-blood-test-using-mirna-beacon-probe
#15
Bindu Salim, M V Athira, A Kandaswamy, Madhulika Vijayakumar, T Saravanan, Thiagarajan Sairam
Breast cancer is identified as the highest cause of death in women suffering from cancer. Early diagnosis is the key to increase the survival of breast cancer victims. Molecular diagnosis using biomarkers have advanced much in the recent years. The cost involved in such diagnosis is not affordable for most of the population. The concept being investigated here is to realize a simple diagnosis system for screening cancer by way of a blood test utilizing a miRNA based biomarker with a complementary molecular beacon probe...
September 30, 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28948399/interventional-magnetic-resonance-imaging-guided-carotid-embolectomy-using-a-novel-resonant-marker-catheter-demonstration-of-preclinical-feasibility
#16
Jeffrey K Yang, Andre M Cote, Caroline D Jordan, Sravani Kondapavulur, Aaron D Losey, David McCoy, Andrew Chu, Jay F Yu, Teri Moore, Carol Stillson, Fabio Settecase, Matthew D Alexander, Andrew Nicholson, Daniel L Cooke, Maythem Saeed, Dave Barry, Alastair J Martin, Mark W Wilson, Steven W Hetts
To assess the visualization and efficacy of a wireless resonant circuit (wRC) catheter system for carotid artery occlusion and embolectomy under real-time MRI guidance in vivo, and to compare MR imaging modality with x-ray for analysis of qualitative physiological measures of blood flow at baseline and after embolectomy. The wRC catheter system was constructed using a MR compatible PEEK fiber braided catheter (Penumbra, Inc, Alameda, CA) with a single insulated longitudinal copper loop soldered to a printed circuit board embedded within the catheter wall...
September 25, 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28948395/passive-wireless-transduction-of-electrochemical-impedance-across-thin-film-microfabricated-coils-using-reflected-impedance
#17
Alex Baldwin, Lawrence Yu, Madelina Pratt, Kee Scholten, Ellis Meng
A new method of wirelessly transducing electrochemical impedance without integrated circuits or discrete electrical components was developed and characterized. The resonant frequency and impedance magnitude at resonance of a planar inductive coil is affected by the load on a secondary coil terminating in sensing electrodes exposed to solution (reflected impedance), allowing the transduction of the high-frequency electrochemical impedance between the two electrodes. Biocompatible, flexible secondary coils with sensing electrodes made from gold and Parylene C were microfabricated and the reflected impedance in response to phosphate-buffered saline solutions of varying concentrations was characterized...
September 25, 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28929363/all-in-one-low-intensity-pulsed-ultrasound-stimulation-system-using-piezoelectric-micromachined-ultrasonic-transducer-pmut-arrays-for-targeted-cell-stimulation
#18
Wonjun Lee, Seungjun Yoo, Joontaek Jung, Woojin Kang, Wei Wang, Cheil Moon, Hongsoo Choi
A novel cell-stimulation system was fabricated using 10 × 29 piezoelectric micromachined ultrasonic transducer (pMUT) arrays for targeted ultrasonic cell stimulation. Both the diameter of a single pMUT element and the edge-to-edge gap were 120 μm, and the size of a pMUT array was 2.27 × 6.84 mm, to be placed at the bottom of a Transwell. The measured resonance frequency of a single pMUT element was 1.48 ± 0.13 MHz and the measured acoustic intensity of the pMUT array was 0.15 ± 0.03 MPa at 1 mm away from the transducer...
September 19, 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28929304/embryonic-body-culturing-in-an-all-glass-microfluidic-device-with-laser-processed-4%C3%A2-%C3%AE-m-thick-ultra-thin-glass-sheet-filter
#19
Y Yalikun, N Tanaka, Y Hosokawa, T Iino, Y Tanaka
In this paper, we report the development and demonstration of a method to fabricate an all-glass microfluidic cell culturing device without circulation flow. On-chip microfluidic cell culturing is an indispensable technique for cellular replacement therapies and experimental cell biology. Polydimethylsiloxane (PDMS) have become a popular material for fabricating microfluidic cell culture devices because it is a transparent, biocompatible, deformable, easy-to-mold, and gas-permeable. However, PDMS is also a chemically and physically unstable material...
September 19, 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28929244/analyzing-polymeric-matrix-for-fabrication-of-a-biodegradable-microneedle-array-to-enhance-transdermal-delivery
#20
Kuo-Yuan Hwa, Vincent H S Chang, Yao-Yi Cheng, Yue-Da Wang, Pey-Shynan Jan, Boopathi Subramani, Min-Ju Wu, Bo-Kai Wang
Traditional drug delivery systems, using invasive, transdermal, and oral routes, are limited by various factors, such as the digestive system environment, skin protection, and sensory nerve stimulation. To improve the drug delivery system, we fabricated a polysaccharide-based, dissolvable microneedle-based array, which combines the advantages of both invasive and transdermal delivery systems, and promises to be an innovative solution for minimally invasive drug delivery. In this study, we designed a reusable aluminum mold that greatly improved the efficiency and convenience of microneedle fabrication...
September 19, 2017: Biomedical Microdevices
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