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Biomedical Microdevices

Weiyu Shen, Yi Song, Alison Burklund, Biao Le, Ru Zhang, Lijie Wang, Yong Xi, Kun Qian, Ting Shen, John X J Zhang
We demonstrate enhanced on-chip circulating tumor cell (CTC) detection through the incorporation of plasmonic-enhanced near-infrared (NIR) fluorescence screening. Specifically, the performance of plasmonic gold coated chips was evaluated on our previously reported immunomagnetic CTC capture system and compared to the performance of a regular chip. Three main performance metrics were evaluated: capture efficiency, capture reproducibility, and clinical efficacy. Use of the plasmonic chip to capture SK-BR-3 cells in PBS, resulted in a capture efficiency of 82%, compared to 76% with a regular chip...
November 12, 2018: Biomedical Microdevices
Frank Bunge, Sander van den Driesche, Michael J Vellekoop
Microfluidic cell cultures are often used in academic research but only rarely in pharmaceutical research because of unsuitable designs, inappropriate choice of materials or incompatibility with standard equipment. In particular, microfluidic cell cultures to control the gaseous microenvironment rely on PDMS despite its disadvantages. We present a novel concept for such a cell culture device that addresses these issues and is made out of hard materials instead of PDMS. Our device contains two microfluidic chambers that are separated by a porous membrane of anodized aluminum oxide...
November 10, 2018: Biomedical Microdevices
Kai Zhang, Guangmeng Xu, Renna Qiu, Lizhe Wang, Jiannan Li, Feng Qian
This study aimed to evaluate the effect of irreversible electroporation (IRE) on the stomach wall after IRE was applied on liver tissues adjacent to the anterior wall of the stomach. IRE ablation was performed in eight Tibet mini-pigs with three lesions per pig. The IRE electrodes were inserted into the liver tissues situated close to the anterior wall of the stomach. As for the control group, the IRE electrodes were also inserted into the liver tissues for three lesions in four Tibet mini-pigs but did not turn on the current...
November 8, 2018: Biomedical Microdevices
Xiang Zhang, Haotong Li, Zhenxing Wang, Xueye Chen, Qian Li
Polymer microfluidic devices are used for many purposes such as microarrays and biochips. The key tool for manufacturing these chips in bulk is an appropriate mold. However, the popular material for making molds is nickel or nickel alloys, which have low stiffness and wear out easily. Zr-based metallic glass is a promising material for micro- or nanomolds because it has good mechanical properties and can be easily formed with high precision. In this paper, Zr-based metallic glass is proposed for use as micromold insert to make poly-(methyl methacrylate) (PMMA) microfluidic devices...
November 6, 2018: Biomedical Microdevices
Mohd Anuar Md Ali, Aminuddin Bin Ahmad Kayani, Leslie Y Yeo, Adam F Chrimes, Muhammad Zamharir Ahmad, Kostya Ken Ostrikov, Burhanuddin Yeop Majlis
Cell contact formation, which is the process by which cells are brought into close proximity is an important biotechnological process in cell and molecular biology. Such manipulation is achieved by various means, among which dielectrophoresis (DEP) is widely used due to its simplicity. Here, we show the advantages in the judicious choice of the DEP microelectrode configuration in terms of limiting undesirable effects of dielectric heating on the cells, which could lead to their inactivation or death, as well as the possibility for cell clustering, which is particularly advantageous over the linear cell chain arrangement typically achieved to date with DEP...
November 6, 2018: Biomedical Microdevices
E Teston, V Hingot, V Faugeras, C Errico, M Bezagu, M Tanter, O Couture
Ultrasound-vaporizable microdroplets can be exploited for targeted drug delivery. However, it requires customized microfluidic techniques able to produce monodisperse, capillary-sized and biocompatible multiple emulsions. Recent development of microfluidic devices led to the optimization of microdroplet production with high yields, low polydispersity and well-defined diameters. So far, only few were shown to be efficient for simple droplets or multiple emulsions production below 5 μm in diameter, which is required to prevent microembolism after intravenous injection...
October 30, 2018: Biomedical Microdevices
Ralph Santos-Oliveira, Michael G Stabin
The use of radioactive nanoparticles as imaging and therapeutic agents is increasing globally. Indeed, the use of these nanoparticles as perfect theranostic agent is highly anticipated in the pharmaceutical market. Among the radioactive nanoparticles, liposomes, solid lipid nanoparticles and polymeric nanoparticles are the most studied. However little information among adverse reactions, absorbed dose and correct dose to achieve the theranostic goal in a translational application is available. We developed a radioactive polymeric nanoparticle and calculated the absorbed dose in animal model (Wistar rats) using the OLINDA/EXM program...
October 29, 2018: Biomedical Microdevices
Sammer-Ul Hassan, Adrian M Nightingale, Xize Niu
Here a micromachined flow cell with enhanced optical sensitivity is presented that allows high-throughput analysis of microdroplets. As a droplet flows through multiple concatenated measurement points, the rate of enzymatic reaction in the droplet can be fully characterized without stopping the flow. Since there is no cross-talk between the droplets, the flow cell is capable of continuously measuring biochemical assays in a droplet flow and thus is suitable to be used for continuous point-of-care diagnostics monitoring...
October 29, 2018: Biomedical Microdevices
Xiaoping Min, Da Fu, Jianzhong Zhang, Juntian Zeng, Zhenyu Weng, Wendi Chen, Shiyin Zhang, Dongxu Zhang, Shengxiang Ge, Jun Zhang, Ningshao Xia
A rapid, sensitive and quantitative biomarker detection platform is of great importance to the small clinic or point-of-care (POC) diagnosis. In this work, we realize that an automated diagnostic platform mainly includes two components: (1) an instrument that can complete all steps of the chemiluminescence immunoassay automatically and (2) an integrated microfluidic chip which is disposable and harmless. In the instrument, we adopt vacuum suction cups which are driven by linear motor to realize a simple, effective and convenient control...
October 25, 2018: Biomedical Microdevices
Kin Fong Lei, Shao-Chieh Hsieh, Andrew Goh, Rei-Lin Kuo, Ngan-Ming Tsang
Elimination of serious side effects is a desired feature of cancer therapy. Alternating electric field treatment is one approach to the non-invasive treatment of cancer. The efficacy and safety of this novel therapy are confirmed for the treatment of glioblastoma multiforme. In the current study, we co-cultured cancer cells and normal cells to investigate the selectivity and chemosensitivity enhancement of an electric field treatment. Cancer cells (cell line: HeLa and Huh7) and fibroblasts (cell line: HEL299) were cultured in an in-house-developed cell culture device embedded with stimulating electrodes...
October 22, 2018: Biomedical Microdevices
Yuan Jia, Hao Sun, Xiao Li, Dongke Sun, Tao Hu, Nan Xiang, Zhonghua Ni
Rapid, disposable, point-of-care (POC) oral fluid testing has gained considerable attention in recent years as saliva contains biomarker and components of the serum proteome that offer important information on both oral and systemic disease. Microfluidic paper-based analytical devices (μPADs) coupled with smartphone reflectance sensing systems have long been considered to be an effective POC tool for the diagnostics of biomarkers in oral fluid. However, the existing portable systems are limited by the poor color distribution in the detection area as well as not being universally applicable...
October 12, 2018: Biomedical Microdevices
Lizhe Wang, Jian Chang, Yaqin Qu, Renna Qiu
Irreversible electroporation (IRE) is a kind of promising cancer treatment technology. However, local recurrence still occurs because of incomplete ablation. The aim of this study was to investigate the combined therapy of IRE and a hydroxycamptothecin loaded electrospun membrane (EM/HCPT) to treat rabbit VX2 subcutaneous cancer. HCPT loaded membranes were developed by electrospinning. Mechanical test and in vitro drug release study of EM/HCPT were performed. 24 rabbits with subcutaneous VX2 tumor were randomly divided into four groups: the control group, the EM/HCPT group, the IRE ablation group, and the IRE + EM/HCPT group...
October 11, 2018: Biomedical Microdevices
Emily N Abada, Benjamin J Feinberg, Shuvo Roy
While extracorporeal membrane oxygenation (ECMO) is a valuable therapy for patients with lung or heart failure, clinical use of ECMO remains limited due to hemocompatibility concerns with pro-coagulatory hollow fiber membrane geometries. Previously, we demonstrated the feasibility of silicon nanopore (SNM) and micropore (SμM) membranes for transport between two liquid-phase compartments in blood-contacting devices. Herein, we investigate various pore sizes of SNM and SμM membranes - alone or with a polydimethylsiloxane (PDMS) protective coating - for parameters that determine suitability for gas exchange...
October 5, 2018: Biomedical Microdevices
Yo-Chang Jang, Hyun-Ju Park, Ayoung Woo, Kyu-Sung Lee, Hui-Sung Moon, Jin Ho Oh, Min-Young Lee
Selective cell enrichment technologies can play an important role in both diagnostic and therapeutic areas. However, currently used cell sorting techniques have difficulties in rapidly isolating only the desired target cells from a large volume of body fluids. In this work, we developed a filtering system that can quickly separate and highly concentrate cells from a large volume of solution, depending on their size, using a silicon membrane filter. To overcome the problems caused by material limitations of the brittle silicon, we designed a novel membrane filter with various pore designs...
October 5, 2018: Biomedical Microdevices
Kazuaki Nagayama, Takuya Inoue, Yasuhiro Hamada, Shukei Sugita, Takeo Matsumoto
Cells change the traction forces generated at their adhesion sites, and these forces play essential roles in regulating various cellular functions. Here, we developed a novel magnetic-driven micropillar array PDMS substrate that can be used for the mechanical stimulation to cellular adhesion sites and for the measurement of associated cellular traction forces. The diameter, length, and center-to-center spacing of the micropillars were 3, 9, and 9 μm, respectively. Sufficient quantities of iron particles were successfully embedded into the micropillars, enabling the pillars to bend in response to an external magnetic field...
September 26, 2018: Biomedical Microdevices
Jacob A M Nuhn, Shenmin Gong, Xiangchen Che, Long Que, Ian C Schneider
The original version of this article unfortunately contained a mistake. One line indicating statistical significance was improperly placed in Fig. 5.
September 18, 2018: Biomedical Microdevices
Feng Liu, Shuibing Wang, Zhigang Lu, Yumei Sun, Chaogang Yang, Qiongwei Zhou, Shaoli Hong, Shengxiang Wang, Bin Xiong, Kan Liu, Nangang Zhang
Isolation and detection of circulating tumor cells (CTCs) has showed a great clinical impact for tumor diagnosis and treatment monitoring. Despite significant progresses of the existing technologies, feasible and cost-effective CTC isolation techniques are more desirable. In this study, a novel method was developed for highly efficient isolation of CTCs from breast cancer patients based on biophysical properties using a pyramid-shaped microchamber. Through optimization tests, the outlet height of 6 μm and the flow rate of 200 μL/min were chosen as the optimal conditions...
September 17, 2018: Biomedical Microdevices
Qian Wu, Xinwei Wei, Yuxiang Pan, Yingchang Zou, Ning Hu, Ping Wang
To perform the drug screening, planar cultured cell models are commonly applied to test efficacy and toxicity of drugs. However, planar cultured cells are different from the human 3D organs or tissues in vivo. To simulate the human 3D organs or tissues, 3D spheroids are developed by culturing a small aggregate of cells which reside around the extracellular matrix and interact with other cells in liquid media. Here we apply lung carcinoma cell lines to engineer the 3D lung cancer spheroid-based biosensor using the interdigitated electrodes for drug efficacy evaluation...
September 15, 2018: Biomedical Microdevices
Yu Zhao, Jozef Brcka, Jacques Faguet, Guigen Zhang
Cell rotation can be achieved by utilizing rotating electric fields through which torques are generated due to phase difference between the dipole moment of cells and the external electric field. While reports of cell rotation under non-rotating electrical fields, such as dielectrophoresis (DEP), are abound, the underlying mechanism is not fully understood. Because of this, contradicting arguments remain regarding if a single cell can rotate under conventional DEP. What's more, the current prevailing DEP theory is not adequate for identifying the cause for such disagreements...
September 8, 2018: Biomedical Microdevices
Jingyun Ma, Na Li, Yachen Wang, Liang Wang, Wenjuan Wei, Liming Shen, Yu Sun, Yang Jiao, Weigong Chen, Jing Liu
3D models of tumours have emerged as an advanced technique in pharmacology and tumour cell biology, in particular for studying malignant tumours such as glioblastoma multiforme (GBM). Herein, we developed a 3D GBM model on a detachably assembled microfluidic device, which could be used to study GBM aggressiveness and for anti-GBM drug testing. Fundamental characteristics of the GBM microenvironment in terms of 3D tissue organisation, extracellular matrices and blood flow were reproduced in vitro by serial manipulations in the integrated microfluidic device, including GBM spheroid self-assembly, embedding in a collagen matrix, and continuous perfusion culture, respectively...
September 6, 2018: Biomedical Microdevices
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