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Biomedical Microdevices

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https://www.readbyqxmd.com/read/28364179/applications-and-theory-of-electrokinetic-enrichment-in-micro-nanofluidic-chips
#1
Xueye Chen, Shuai Zhang, Lei Zhang, Zhen Yao, Xiaodong Chen, Yue Zheng, Yanlin Liu
This review reports the progress on the recent development of electrokinetic enrichment in micro-nanofluidic chips. The governing equations of electrokinetic enrichment in micro-nanofluidic chips are given. Various enrichment applications including protein analysis, DNA analysis, bacteria analysis, viruses analysis and cell analysis are illustrated and discussed. The advantages and difficulties of each enrichment method are expatiated. This paper will provide a particularly convenient and valuable reference to those who intend to research the electrokinetic enrichment based on micro-nanofluidic chips...
September 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28357654/human-adipocyte-differentiation-and-characterization-in-a-perfusion-based-cell-culture-device
#2
Yunxiao Liu, Patthara Kongsuphol, Sajay Bhuvanendran Nair Gourikutty, Qasem Ramadan
Adipocytes have gained significant attention recently, because they are not only functioning as energy storage but also as endocrine cells. Adipocytes secret various signaling molecules, including adiponectin, MCP-1, and IL-6, termed collectively as "adipokines". Adipokines regulate glucose metabolism, thereby play an important role in obesity, diabetes type 2, and other metabolic disorders. Conventionally, to study the secretory function, adipocytes are cultured in vitro in static conditions. However, static culturing condition falls short of mimicking the interstitial fluid flows in living systems...
September 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28357653/identifying-drug-resistant-cancer-cells-using-microbubble-well-arrays
#3
Qihui Pu, Ryan Spooner, Lisa A DeLouise
Drug resistance is a characteristic of tumor initiating cells that can give rise to metastatic disease. In this work we demonstrate the use of microbubble well arrays as a cell culture platform to enumerate and characterize drug resistant cells in a human derived tumorigenic squamous cell carcinoma cell line. The spherical architecture and compliant hydrophobic composition of the microbubble well favors single cell survival, clonal proliferation and formation of spheres that do not grow on standard tissue culture plastic and are resistant to cisplatin...
September 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28357652/optimized-acoustic-biochip-integrated-with-microfluidics-for-biomarkers-detection-in-molecular-diagnostics
#4
G Papadakis, J M Friedt, M Eck, D Rabus, G Jobst, E Gizeli
The development of integrated platforms incorporating an acoustic device as the detection element requires addressing simultaneously several challenges of technological and scientific nature. The present work was focused on the design of a microfluidic module, which, combined with a dual or array type Love wave acoustic chip could be applied to biomedical applications and molecular diagnostics. Based on a systematic study we optimized the mechanics of the flow cell attachment and the sealing material so that fluidic interfacing/encapsulation would impose minimal losses to the acoustic wave...
September 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28432532/a-portable-battery-powered-microfluidic-impedance-cytometer-with-smartphone-readout-towards-personal-health-monitoring
#5
Niloy Talukder, Abbas Furniturewalla, Tuan Le, Matthew Chan, Shreyas Hirday, Xinnan Cao, Pengfei Xie, Zhongtian Lin, Azam Gholizadeh, Steve Orbine, Mehdi Javanmard
We present a portable system for personalized blood cell counting consisting of a microfluidic impedance cytometer and portable analog readout electronics, feeding into an analog-to-digital converter (ADC), and being transmitted via Bluetooth to a user-accessible mobile application. We fabricated a microfluidic impedance cytometer with a novel portable analog readout. The novel design of the analog readout, which consists of a lock-in-amplifier followed by a high-pass filter stage for subtraction of drift and DC offset, and a post-subtraction high gain stage, enables detection of particles and cells as small as 1 μm in diameter, despite using a low-end 8-bit ADC...
June 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28432531/effectiveness-of-nanoencapsulated-methotrexate-against-osteosarcoma-cells-in-vitro-cytotoxicity-under-dynamic-conditions
#6
O Mitxelena-Iribarren, C L Hisey, M Errazquin-Irigoyen, Y González-Fernández, E Imbuluzqueta, M Mujika, M J Blanco-Prieto, S Arana
Cancer is a leading cause of mortality in the world, with osteosarcoma being one of the most common types among children between 1 and 14 years old. Current treatments including preoperative chemotherapy, surgery and postoperative chemotherapy produce several side effects with limited effectiveness. The use of lipid nanoparticles as biodegradable shells for controlled drug delivery shows promise as a more effective and targeted tumor treatment. However, in vitro validation of these vehicles is limited due to fluid stagnation in current techniques, in which nanoparticles sediment onto the bottom of the wells killing the cells by asphyxiation...
June 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28432530/an-integrated-microsystem-with-dielectrophoresis-enrichment-and-impedance-detection-for-detection-of-escherichia-coli
#7
Renjie Wang, Yi Xu, Haitao Liu, Jinlan Peng, Joseph Irudayaraj, Feiyun Cui
An integrated microsystem device with matched interdigitated microelectrode chip was fabricated for enrichment and detection of Escherichia coli O157:H7. The microsystem has integrated with positive dielectrophoresis (pDEP) enrichment and in situ impedance detection, whose total volume is only 3.0 × 10(-3) m(3), and could provide impedance testing voltages of 0 ~ 10 V, detection frequencies of 1 KHz ~ 1 MHz, DEP excitation signals with amplitude of 0 ~ 10 Vpp and frequencies of 1KHz ~ 1 MHz, which fully meets the demands of pDEP enrichment and impedance detection for bacteria...
June 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28429183/label-free-okadaic-acid-detection-using-growth-of-gold-nanoparticles-in-sensor-gaps-as-a-conductive-tag
#8
Yuxiang Pan, Zijian Wan, Longjie Zhong, Xueqin Li, Qi Wu, Jun Wang, Ping Wang
Okadaic acid (OA) is a marine toxin ingested by shellfish. In this work, a simple, sensitive and label-free gap-based electrical competitive bioassay has been developed for this biotoxin detection. The gap-electrical biosensor is constructed by modifying interdigitated microelectrodes with gold nanoparticles (AuNPs) and using the self-catalytic growth of AuNPs as conductive bridges. In this development, the AuNPs growth is realized in the solution of glucose and chloroauric acid, with glucose oxidation used as the catalysis for growth of the AuNPs...
June 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28425028/energy-harvesting-from-cerebrospinal-fluid-pressure-fluctuations-for-self-powered-neural-implants
#9
Levent Beker, Arnau Benet, Ali Tayebi Meybodi, Ben Eovino, Albert P Pisano, Liwei Lin
In this paper, a novel method to generate electrical energy by converting available mechanical energy from pressure fluctuations of the cerebrospinal fluid within lateral ventricles of the brain is presented. The generated electrical power can be supplied to the neural implants and either eliminate their battery need or extend the battery lifespan. A diaphragm type harvester comprised of piezoelectric material is utilized to convert the pressure fluctuations to electrical energy. The pressure fluctuations cause the diaphragm to bend, and the strained piezoelectric materials generate electricity...
June 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28421401/liquid-marbles-as-bioreactors-for-the-study-of-three-dimensional-cell-interactions
#10
Raja K Vadivelu, Harshad Kamble, Ahmed Munaz, Nam-Trung Nguyen
Liquid marble as a bioreactor platform for cell-based studies has received significant attention, especially for developing 3D cell-based assays. This platform is particularly suitable for 3D in-vitro modeling of cell-cell interactions. For the first time, we demonstrated the interaction of olfactory ensheathing cells (OECs) with nerve debris and meningeal fibroblast using liquid marbles. As the transplantation of OECs can be used for repairing nerve injury, degenerating cell debris within the transplantation site can adversely affect the survival of transplanted OECs...
June 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28421400/a-multistage-dialysis-microdevice-for-extraction-of-cryoprotectants
#11
Lili Zou, Shibo Li, Yufeng Kang, Juanjuan Liu, Liqun He, Sijie Sun, Dayong Gao, Bensheng Qiu, Weiping Ding
In this study, we present a multistage-dialysis microdevice (MDM) for extraction of cryoprotectants (CPAs) from a CPA-laden cell suspension. We confirmed the functions of the key designs of the MDM using a fluorescence solution, we assessed the performance of the MDM by using the MDM to unload glycerin from glycerin-loaded swine erythrocytes, and we investigated the effects of the cell suspension flow rate, glycerin concentration, cell density, and membrane pore size on the clearance efficiency of glycerin (CG), the survival rate of cells (SC), and the recovery rate of cells (RC)...
June 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28417251/irreversible-electroporation-for-the-treatment-of-rabbit-vx2-breast-cancer
#12
Wenlong Zhang, Wei Chai, Jianying Zeng, Jibing Chen, Liqi Bi, Lizhi Niu
Irreversible electroporation (IRE) is considered predominantly as a non-thermal ablative technique that uses electrical fields to permeabilize cell membranes and lead to cell death. In this study, we evaluated the efficacy of IRE in the rabbit VX2 breast cancer model. Thirty-five female New Zealand white rabbits were inoculated against VX2 breast cancer cells. The rabbits were randomly divided into two groups: a control group of 15 rabbits and an IRE treatment group of 20 rabbits. Treatment and treatment outcome were evaluated by computerized tomography (CT) scan (plain or contrast enhanced), tumor growth curves and pathological examination including H&E, TUNEL, PCNA and CD31 staining...
June 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28417250/impact-of-surface-roughness-on-dielectrophoretically-assisted-concentration-of-microorganisms-over-pcb-based-platforms
#13
Geeta Bhatt, Rishi Kant, Keerti Mishra, Kuldeep Yadav, Deepak Singh, Ramanathan Gurunath, Shantanu Bhattacharya
This article presents a PCB based microfluidic platform for performing a dielectrophoretic capture of live microorganisms over inter-digitated electrodes buried under layers of different surface roughness values. Although dielectrophoresis has been extensively studied earlier over silicon and polymer surfaces with printed electrodes the issue of surface roughness particularly in case of buried electrodes has been seldom investigated. We have addressed this issue through a layer of spin coated PDMS (of various surface roughness) that is used to cover the printed electrodes over a printed circuit board...
June 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28405868/droplet-based-non-faradaic-impedance-sensors-for-assessment-of-susceptibility-of-escherichia-coli-to-ampicillin-in-60-min
#14
Aida Ebrahimi, Muhammad A Alam
Direct antibiotic susceptibility tests (AST) are essential for rapid detection of bacterial infection and administration of appropriate antibiotics. Conventional AST systems are usually slow as they rely on cell growth for an indirect assessment of antibiotics' effectiveness. Therefore, a faster method is desirable, especially for emergency cases. In this work, we studied the performance of label-free, droplet-based impedance sensors for rapid characterization of the effects of ampicillin (Amp) on Escherichia coli...
June 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28391436/parylene-scaffold-for-cartilage-lesion
#15
Carlos Eduardo da Silveira Franciozi, Carleton Thomas Vangsness, James Eugene Tibone, Juan Carlos Martinez, Damien Rodger, Tzu-Chieh Chou, Yu-Chong Tai, Rodrigo Brant, Ling Wu, Rene Jorge Abdalla, Bo Han, Denis Evseenko, Mark Humayun
Evaluate parylene scaffold feasibility in cartilage lesion treatment, introducing a novel paradigm combining a reparative and superficial reconstructive procedure. Fifteen rabbits were used. All animals had both knees operated and the same osteochondral lesion model was created bilaterally. The parylene scaffold was implanted in the right knee, and the left knee of the same animal was used as control. The animals were euthanized at different time points after surgery: four animals at three weeks, three animals at six weeks, four animals at nine weeks, and four animals at 12 weeks...
June 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28378147/density-gradient-assisted-centrifugal-microfluidics-an-approach-to-continuous-mode-particle-separation
#16
Yoshiaki Ukita, Takayuki Oguro, Yuzuru Takamura
Centrifugal microfluidics has been recognized as a promising pumping method in microfluidics because of its simplicity, easiness of automation, and parallel processing. However, the patterning of stripe flow in centrifugal microfluidics is challenging because a fluid is significantly affected by the Coriolis force, which produces an intrinsic secondary flow. This paper reports a technical and design strategy for centrifugal microfluidics called "density-gradient-assisted centrifugal microfluidics." The flow behavior is observed with the presence of a density gradient and without a density gradient in two concentrically traveling phase flows...
June 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28378146/use-of-a-highly-parallel-microfluidic-flow-cell-array-to-determine-therapeutic-drug-dose-response-curves
#17
Jesús A Arellano, Taylor A Howell, James Gammon, Sungpil Cho, Margit M Janát-Amsbury, Bruce Gale
A high-throughput, microfluidic flow cell array (MFCA) system has been modified to enable drug screening against small-volume cell-, and tissue cultures. The MFCA is composed of a 3D channel network that simultaneously flows fluids through forty-eight 830 μm by 500 μm flow cells, which physically divide and fluidically seal an existing culture into multiple compartments when docked onto the surface of a cell or tissue culture dish. The modified system provides temperature (37 °C) and CO2/pH level controls, while continuously flowing solutions (media or other liquid such as drug suspensions) over the cells/tissues...
June 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28374278/microbubble-activated-acoustic-cell-sorting
#18
M A Faridi, H Ramachandraiah, I Iranmanesh, D Grishenkov, M Wiklund, A Russom
Acoustophoresis, the ability to acoustically manipulate particles and cells inside a microfluidic channel, is a critical enabling technology for cell-sorting applications. However, one of the major impediments for routine use of acoustophoresis at clinical laboratory has been the reliance on the inherent physical properties of cells for separation. Here, we present a microfluidic-based microBubble-Activated Acoustic Cell Sorting (BAACS) method that rely on the specific binding of target cells to microbubbles conjugated with specific antibodies on their surface for continuous cell separation using ultrasonic standing wave...
June 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28374277/construction-of-3d-multicellular-microfluidic-chip-for-an-in-vitro-skin-model
#19
Sojin Lee, Seon-Pil Jin, Yeon Kyung Kim, Gun Yong Sung, Jin Ho Chung, Jong Hwan Sung
Current in vitro skin models do not recapitulate the complex architecture and functions of the skin tissue. In particular, on-chip construction of an in vitro model comprising the epidermis and dermis layer with vascular structure for mass transport has not been reported yet. In this study, we aim to develop a microfluidic, three-dimensional (3D) skin chip with fluidic channels using PDMS and hydrogels. Mass transport within the collagen hydrogel matrix was verified with fluorescent model molecules, and a transport-reaction model of oxygen and glucose inside the skin chip was developed to aid the design of the microfluidic skin chip...
June 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28367600/microfluidic-processing-of-synovial-fluid-for-cytological-analysis
#20
John C Krebs, Yunus Alapan, Barbara A Dennstedt, Glenn D Wera, Umut A Gurkan
Cytological analysis of synovial fluid is widely used in the clinic to assess joint health and disease. However, in general practice, only the total number of white blood cells (WBCs) are available for cytologic evaluation of the joint. Moreover, sufficient volume of synovial aspirates is critical to run conventional analyses, despite limited volume of aspiration that can normally be obtained from a joint. Therefore, there is a lack of consistent and standardized synovial fluid cytological tests in the clinic...
June 2017: Biomedical Microdevices
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