Sze Ying Ong, Ralf Schuelein, Rachelia R Wibawa, Daniel W Thomas, Yanny Handoko, Saskia Freytag, Melanie Bahlo, Kaylene J Simpson, Elizabeth L Hartland
The Dot/Icm system of Legionella pneumophila is essential for virulence and delivers a large repertoire of effectors into infected host cells to create the Legionella containing vacuole. Since the secretion of effectors via the Dot/Icm system does not occur in the absence of host cells, we hypothesized that host factors actively participate in Dot/Icm effector translocation. Here we employed a high-throughput, genome-wide siRNA screen to systematically test the effect of silencing 18,120 human genes on translocation of the Dot/Icm effector, RalF, into HeLa cells...
May 26, 2021: Cellular Microbiology