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Genetics and Molecular Biology

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https://www.readbyqxmd.com/read/29111566/complete-sequence-and-comparative-analysis-of-the-chloroplast-genome-of-plinia-trunciflora
#1
Maria Eguiluz, Priscila Mary Yuyama, Frank Guzman, Nureyev Ferreira Rodrigues, Rogerio Margis
Plinia trunciflora is a Brazilian native fruit tree from the Myrtaceae family, also known as jaboticaba. This species has great potential by its fruit production. Due to the high content of essential oils in their leaves and of anthocyanins in the fruits, there is also an increasing interest by the pharmaceutical industry. Nevertheless, there are few studies focusing on its molecular biology and genetic characterization. We herein report the complete chloroplast (cp) genome of P. trunciflora using high-throughput sequencing and compare it to other previously sequenced Myrtaceae genomes...
November 6, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/29111565/transcriptome-sequencing-analysis-of-alfalfa-reveals-cbf-genes-potentially-playing-important-roles-in-response-to-freezing-stress
#2
Yongjun Shu, Wei Li, Jinyue Zhao, Sijia Zhang, Hanyun Xu, Ying Liu, Changhong Guo
Alfalfa (Medicago sativa L.) is an important perennial forage, with high nutritional value, which is widely grown in the world. Because of low freezing tolerance, its distribution and production are threatened and limited by winter weather. To understand the complex regulation mechanisms of freezing tolerance in alfalfa, we performed transcriptome sequencing analysis under cold (4 °C) and freezing (-8 °C) stresses. More than 66 million reads were generated, and we identified 5767 transcripts differentially expressed in response to cold and/or freezing stresses...
November 6, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/29111564/relationship-between-xpd-rad51-and-apex1-dna-repair-genotypes-and-prostate-cancer-risk-in-the-male-population-of-rio-de-janeiro-brazil
#3
Ana Sheila Cypriano, Gilda Alves, Antonio Augusto Ornellas, José Scheinkman, Renata Almeida, Luciano Scherrer, Claudia Lage
Susceptibility to cancer ensues in individuals carrying malfunctioning DNA repair mechanisms. The impact of Single Nucleotide Polymorphisms (SNPs) in key DNA repair mechanisms on risk for prostate cancer was investigated in this case-control study. Samples consisted of 110 patients with confirmed prostate cancer and 200 unaffected men, from Rio de Janeiro, Brazil. XPD/Lys751Gln (rs13181), APEX1/Asp148Glu (rs1130409), and RAD51/G135C (rs1801320) SNPs were analyzed by PCR-RFLP. Allelic and genotypic frequencies were calculated and compared by Chi-Square test...
November 6, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/29111563/multiple-genes-contribute-to-anhydrobiosis-tolerance-to-extreme-desiccation-in-the-nematode-panagrolaimus-superbus
#4
Cláudia Carolina Silva Evangelista, Giovanna Vieira Guidelli, Gustavo Borges, Thais Fenz Araujo, Tiago Alves Jorge de Souza, Ubiraci Pereira da Costa Neves, Alan Tunnacliffe, Tiago Campos Pereira
The molecular basis of anhydrobiosis, the state of suspended animation entered by some species during extreme desiccation, is still poorly understood despite a number of transcriptome and proteome studies. We therefore conducted functional screening by RNA interference (RNAi) for genes involved in anhydrobiosis in the holo-anhydrobiotic nematode Panagrolaimus superbus. A new method of survival analysis, based on staining, and proof-of-principle RNAi experiments confirmed a role for genes involved in oxidative stress tolerance, while a novel medium-scale RNAi workflow identified a further 40 anhydrobiosis-associated genes, including several involved in proteostasis, DNA repair and signal transduction pathways...
November 6, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/29111562/expression-analysis-on-14-3-3-proteins-in-regenerative-liver-following-partial-hepatectomy
#5
Deming Xue, Yang Xue, Zhipeng Niu, Xueqiang Guo, Cunshuan Xu
14-3-3 proteins play a vital part in the regulation of cell cycle and apoptosis as signaling integration points. During liver regeneration, the quiescent hepatocytes go through hypertrophy and proliferation to restore liver weight. Therefore, we speculated that 14-3-3 proteins regulate the progression of liver regeneration. In this study, we analyzed the expression patterns of 14-3-3 proteins during liver regeneration of rat to provide an insight into the regenerative mechanism using western blotting. Only four isoforms (γ, ε, σ and τ/θ) of the 14-3-3 proteins were expressed in regenerative liver after partial hepatectomy (PH)...
November 6, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/29111561/gstt1-and-gstm1-null-variants-in-mestizo-and-amerindian-populations-from-northwestern-mexico-and-a-literature-review
#6
Luz Elena Palma-Cano, Emilio J Córdova, Lorena Orozco, Angélica Martínez-Hernández, Miguel Cid, Irene Leal-Berumen, Angel Licón-Trillo, Ruth Lechuga-Valles, Mauricio González-Ponce, Everardo González-Rodríguez, Verónica Moreno-Brito
The GSTT1 and GSTM1 genes are key molecules in cellular detoxification. Null variants in these genes are associated with increase susceptibility to developing different types of cancers. The aim of this study was to determine the prevalence of GSTT1 and GSTM1 null genotypes in Mestizo and Amerindian individuals from the Northwestern region of Mexico, and to compare them with those reported worldwide. GSTT1 and GSTM1 null variants were genotyped by multiplex PCR in 211 Mestizos and 211 Amerindian individuals...
November 6, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/29111560/identification-of-a-novel-mutation-in-arsa-gene-in-three-patients-of-an-iranian-family-with-metachromatic-leukodystrophy-disorder
#7
Neda Golchin, Mohammadreza Hajjari, Reza Azizi Malamiri, Majid Aminzadeh, Javad Mohammadi-Asl
Metachromatic leukodystrophy disorder (MLD) is an autosomal recessive and lysosomal storage disease. The disease is caused by the deficiency of the enzyme arylsulfatase A (ARSA) which is encoded by the ARSA gene. Different mutations have been reported in different populations. The present study was aimed to detect the mutation type of the ARSA gene in three relative Iranian patients. We found a novel homozygous missense mutation c.1070 G > T (p.Gly357Val) in exon 6 of these patients. The mutation was found to be reported for the first time in MLD patients...
November 6, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/29064514/comparative-transcriptome-profile-of-the-leaf-elongation-zone-of-wild-barley-hordeum-spontaneum-eibi1-mutant-and-its-isogenic-wild-type
#8
Qin Zhou, Aidong Wang, Ruijun Duan, Jun Yan, Gang Zhao, Eviatar Nevo, Guoxiong Chen
The naturally occurring wild barley mutant eibi1/hvabcg31 suffers from severe water loss due to the permeable leaf cuticle. Eibi1/HvABCG31 encodes a full ATP-binding cassette (ABC) transporter, HvABCG31, playing a role in cutin deposition in the elongation zone of growing barley leaves. The eibi1 allele has pleiotropic effects on the appearance of leaves, plant stature, fertility, spike and grain size, and rate of germination. Comparative transcriptome profile of the leaf elongation zone of the eibi1 mutant as well as its isogenic wild type showed that various pathogenesis-related genes were up-regulated in the eibi1 mutant...
October 23, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/29064513/the-complete-mitochondrial-genome-of-engyodontium-album-and-comparative-analyses-with-ascomycota-mitogenomes
#9
Xiao-Long Yuan, Xin-Xin Mao, Xin-Min Liu, Sen Cheng, Peng Zhang, Zhong-Feng Zhang
Engyodontium album is a widespread pathogen that causes different kinds of dermatoses and respiratory tract diseases in humans and animals. In spite of its perniciousness, the basic genetic and molecular background of this species remains poorly understood. In this study, the mitochondrial genome sequence of E. album was determined using a high-throughput sequencing platform. The circular mitogenome was found to be 28,081 nucleotides in length and comprised of 17 protein-coding genes, 24 tRNA genes, and 2 rRNA genes...
October 23, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/29064512/adiponectin-promoter-polymorphisms-are-predictors-of-lipid-profile-improvement-after-bariatric-surgery
#10
Aline Simas Gasparotto, Diego Olschowsky Borges, Marília Remuzzi Zandoná, Mauricio Jacques Ramos, Nelson Guardiola Meihnardt, Vanessa S Mattevi
Our aim was to investigate if single nucleotide polymorphisms (SNPs) located in the 5' regions of leptin (LEP, -2548 G > A, rs7799039), resistin (RETN, -420 C > G, rs1862513) and adiponectin (ADIPOQ, -11391 G > A, rs17300539 and -11377 C > G, rs266729) genes were related to changes in body mass index (BMI) and metabolic variables after bariatric surgery in 60 extremely obese individuals. At baseline, ADIPOQ -11391 A-allele carriers showed higher plasma adiponectin and lower total cholesterol levels when compared to G/G homozygotes...
October 23, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/29064511/mapping-qtls-for-drought-tolerance-in-a-sea-5-x-and-277-common-bean-cross-with-ssrs-and-snp-markers
#11
Boris Briñez, Juliana Morini Küpper Cardoso Perseguini, Juliana Santa Rosa, Denis Bassi, João Guilherme Ribeiro Gonçalves, Caléo Almeida, Jean Fausto de Carvalho Paulino, Matthew Ward Blair, Alisson Fernando Chioratto, Sérgio Augusto Morais Carbonell, Paula Arielle Mendes Ribeiro Valdisser, Rosana Pereira Vianello, Luciana Lasry Benchimol-Reis
The common bean is characterized by high sensitivity to drought and low productivity. Breeding for drought resistance in this species involves genes of different genetic groups. In this work, we used a SEA 5 x AND 277 cross to map quantitative trait loci associated with drought tolerance in order to assess the factors that determine the magnitude of drought response in common beans. A total of 438 polymorphic markers were used to genotype the F8 mapping population. Phenotyping was done in two greenhouses, one used to simulate drought and the other to simulate irrigated conditions...
October 23, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/29064510/biomolecular-computers-with-multiple-restriction-enzymes
#12
Sebastian Sakowski, Tadeusz Krasinski, Jacek Waldmajer, Joanna Sarnik, Janusz Blasiak, Tomasz Poplawski
The development of conventional, silicon-based computers has several limitations, including some related to the Heisenberg uncertainty principle and the von Neumann "bottleneck". Biomolecular computers based on DNA and proteins are largely free of these disadvantages and, along with quantum computers, are reasonable alternatives to their conventional counterparts in some applications. The idea of a DNA computer proposed by Ehud Shapiro's group at the Weizmann Institute of Science was developed using one restriction enzyme as hardware and DNA fragments (the transition molecules) as software and input/output signals...
October 23, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/28981563/identification-of-genes-related-to-high-royal-jelly-production-in-the-honey-bee-apis-mellifera-using-microarray-analysis
#13
Hongyi Nie, Xiaoyan Liu, Jiao Pan, Wenfeng Li, Zhiguo Li, Shaowu Zhang, Shenglu Chen, Xiaoqing Miao, Nenggan Zheng, Songkun Su
China is the largest royal jelly producer and exporter in the world, and high royal jelly-yielding strains have been bred in the country for approximately three decades. However, information on the molecular mechanism underlying high royal jelly production is scarce. Here, a cDNA microarray was used to screen and identify differentially expressed genes (DEGs) to obtain an overview on the changes in gene expression levels between high and low royal jelly producing bees. We developed a honey bee gene chip that covered 11,689 genes, and this chip was hybridised with cDNA generated from RNA isolated from heads of nursing bees...
October 2, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/28981562/rare-%C3%AE-0-thalassemia-deletions-detected-by-mlpa-in-five-unrelated-brazilian-patients
#14
Natália O Mota, Elza M Kimura, Roberta D Ferreira, Gisele A Pedroso, Dulcinéia M Albuquerque, Daniela M Ribeiro, Magnun N N Santos, Cristina M Bittar, Fernando F Costa, Maria de Fatima Sonati
Alpha-thalassemias are among the most common genetic diseases in the world. They are characterized by hypochromic and microcytic anemia and great clinical variability, ranging from a practically asymptomatic phenotype to severe anemia, which can lead to intrauterine or early neonatal death. Deletions affecting the α-globin genes, located on chromosome 16p13.3, are the main causes of α-thalassemia. Multiplex ligation-dependent probe amplification (MLPA) can be used to detect rearrangements that cause α-thalassemia, particularly large deletions involving the whole α cluster and/or deletions in the HS-40 region...
October 2, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/28981561/genetic-diversity-of-the-pampas-deer-ozotoceros-bezoarticus-population-in-the-brazilian-pantanal-assessed-by-combining-fresh-fecal-dna-analysis-and-a-set-of-heterologous-microsatellite-loci
#15
Aline Meira Bonfim Mantellatto, Renato Caparroz, Maurício Durante Christofoletti, Ubiratan Piovezan, José Maurício Barbanti Duarte
The pampas deer (Ozotoceros bezoarticus) is close to being classified as 'globally threatened', with the largest population occurring in the Brazilian Pantanal. Since capture is stressful to these animals, non-invasive sampling methods such as the use of feces can provide reliable sources of DNA. The aim of this study was to use fecal samples to evaluate the genetic variability of the Brazilian Pantanal population of pampas deer. Six heterologous microsatellite markers were used to screen 142 stool specimens...
October 2, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/28981560/dna-sampling-from-eggshells-and-microsatellite-genotyping-in-rare-tropical-birds-case-study-on-brazilian-merganser
#16
Thais Augusta Maia, Sibelle Torres Vilaça, Luciana Resende da Silva, Fabricio Rodrigues Santos, Gisele Pires de Mendonça Dantas
This study shows that sampling maternal DNA from hatched and abandoned eggshells is a viable noninvasive strategy for studying the genetics of rare or endangered tropical birds, as exemplified here by the Brazilian Merganser (Mergus octosetaceus). Eighteen microsatellites were isolated from enriched libraries and nine heterologous loci from related species were tested. Seven loci were amplified successfully, with five of them being polymorphic. These loci exhibited amplicons ranging from 110 to 254 bp for 132 samples, with 60 from eggshells and 72 from blood or muscle samples...
October 2, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/28981559/description-of-the-karyotypes-of-stejneger-s-beaked-whale-mesoplodon-stejnegeri-and-hubbs-beaked-whale-m-carlhubbsi
#17
Nozomi Kurihara, Yuko Tajima, Tadasu K Yamada, Ayaka Matsuda, Takashi Matsuishi
The genus Mesoplodon (Cetacea: Odontoceti: Ziphiidae) is one of the few cetacean genera with the karyotype 2n = 42. The 2n = 42 karyotype of M. europaeus and M. carlhubbsi is largely consistent with the general cetacean karyotype 2n = 44, although other 2n = 42 karyotypes do not exhibit clear homologies with the general cetacean karyotype. Therefore, the chromosomes of Mesoplodon species may be the key to understanding cetacean karyological evolution. In the present study, the male karyotypes of M. stejnegeri and M...
October 2, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/28981558/association-of-polymorphisms-in-the-heparanase-gene-hpse-with-hepatocellular-carcinoma-in-chinese-populations
#18
Lixia Yu, Xiaoai Zhang, Yun Zhai, Hongxing Zhang, Wei Yue, Xiumei Zhang, Zhifu Wang, Hong Zhou, Gangqiao Zhou, Feng Gong
Heparanase activity is involved in cancer growth and development in humans and single nucleotide polymorphisms (SNPs) in the heparanase gene (HPSE) have been shown to be associated with tumors. In this study, we investigated whether SNPs in HPSE were a risk factor for hepatocellular carcinoma (HCC) by undertaking a comprehensive haplotype-tagging, case-control study. For this, six haplotype-tagging SNPs (htSNPs) in HPSE were genotyped in 400 HCC patients and 480 controls by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis...
October 2, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/28956891/a-novel-de-novo-col1a1-mutation-in-a-thai-boy-with-osteogenesis-imperfecta-born-to-consanguineous-parents
#19
Siraprapa Tongkobpetch, Noppachart Limpaphayom, Apiruk Sangsin, Thantrira Porntaveetus, Kanya Suphapeetiporn, Vorasuk Shotelersuk
Osteogenesis imperfecta (OI) is genetically heterogeneous. Mutations in COL1A1 and COL1A2 are responsible for at least 90% of the cases, which are transmitted in an autosomal dominant manner or are de novo events. We identified a Thai boy with OI whose parents were first cousins. Because the proband was the product of a consanguineous marriage, we hypothesized that he might be homozygous for a mutation in a known gene causing a recessive form of OI. Using whole exome sequencing (WES), we did not find any pathogenic mutations in any known gene responsible for an autosomal recessive form of OI...
September 21, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/28898354/comparative-genome-wide-polymorphic-microsatellite-markers-in-antarctic-penguins-through-next-generation-sequencing
#20
Juliana A Vianna, Daly Noll, Isidora Mura-Jornet, Paulina Valenzuela-Guerra, Daniel González-Acuña, Cristell Navarro, David E Loyola, Gisele P M Dantas
Microsatellites are valuable molecular markers for evolutionary and ecological studies. Next generation sequencing is responsible for the increasing number of microsatellites for non-model species. Penguins of the Pygoscelis genus are comprised of three species: Adélie (P. adeliae), Chinstrap (P. antarcticus) and Gentoo penguin (P. papua), all distributed around Antarctica and the sub-Antarctic. The species have been affected differently by climate change, and the use of microsatellite markers will be crucial to monitor population dynamics...
July 2017: Genetics and Molecular Biology
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