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Genetics and Molecular Biology

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https://www.readbyqxmd.com/read/28399195/shaping-a-plant-genetics-and-molecular-biology-research-community-in-brazil
#1
EDITORIAL
Marie-Anne Van Sluys
No abstract text is available yet for this article.
April 10, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/28399194/interactions-between-plant-hormones-and-heavy-metals-responses
#2
Lauro Bücker-Neto, Ana Luiza Sobral Paiva, Ronei Dorneles Machado, Rafael Augusto Arenhart, Marcia Margis-Pinheiro
Heavy metals are natural non-biodegradable constituents of the Earth's crust that accumulate and persist indefinitely in the ecosystem as a result of human activities. Since the industrial revolution, the concentration of cadmium, arsenic, lead, mercury and zinc, amongst others, have increasingly contaminated soil and water resources, leading to significant yield losses in plants. These issues have become an important concern of scientific interest. Understanding the molecular and physiological responses of plants to heavy metal stress is critical in order to maximize their productivity...
April 10, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/28399193/high-genetic-diversity-among-and-within-bitter-manioc-varieties-cultivated-in-different-soil-types-in-central-amazonia
#3
Alessandro Alves-Pereira, Nivaldo Peroni, Marcelo Mattos Cavallari, Maristerra R Lemes, Maria Imaculada Zucchi, Charles R Clement
Although manioc is well adapted to nutrient-poor Oxisols of Amazonia, ethnobotanical observations show that bitter manioc is also frequently cultivated in the highly fertile soils of the floodplains and Amazonian dark earths (ADE) along the middle Madeira River. Because different sets of varieties are grown in each soil type, and there are agronomic similarities between ADE and floodplain varieties, it was hypothesized that varieties grown in ADE and floodplain were more closely related to each other than either is to varieties grown in Oxisols...
April 10, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/28399192/genome-wide-identification-classification-and-transcriptional-analysis-of-nitrate-and-ammonium-transporters-in-coffea
#4
Tiago Benedito Dos Santos, Joni Esrom Lima, Mariane Silva Felicio, João Danillo Moura Soares, Douglas Silva Domingues
Nitrogen (N) is quantitatively the main nutrient required by coffee plants, with acquisition mainly by the roots and mostly exported to coffee beans. Nitrate (NO3-) and ammonium (NH4+) are the most important inorganic sources for N uptake. Several N transporters encoded by different gene families mediate the uptake of these compounds. They have an important role in source preference for N uptake in the root system. In this study, we performed a genome-wide analysis, including in silico expression and phylogenetic analyses of AMT1, AMT2, NRT1/PTR, and NRT2 transporters in the recently sequenced Coffea canephora genome...
April 10, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/28350038/regulation-of-na-and-k-homeostasis-in-plants-towards-improved-salt-stress-tolerance-in-crop-plants
#5
Diego M Almeida, M Margarida Oliveira, Nelson J M Saibo
Soil salinity is a major abiotic stress that results in considerable crop yield losses worldwide. However, some plant genotypes show a high tolerance to soil salinity, as they manage to maintain a high K+/Na+ ratio in the cytosol, in contrast to salt stress susceptible genotypes. Although, different plant genotypes show different salt tolerance mechanisms, they all rely on the regulation and function of K+ and Na+ transporters and H+ pumps, which generate the driving force for K+ and Na+ transport. In this review we will introduce salt stress responses in plants and summarize the current knowledge about the most important ion transporters that facilitate intra- and intercellular K+ and Na+ homeostasis in these organisms...
March 27, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/28350037/isolation-and-characterization-of-a-promoter-responsive-to-salt-osmotic-and-dehydration-stresses-in-soybean
#6
Alessandra Jordano Conforte, Fábia Guimarães-Dias, Anna Cristina Neves-Borges, Marta Bencke-Malato, Durvalina Felix-Whipps, Márcio Alves-Ferreira
Drought stress is the main limiting factor of soybean yield. Currently, genetic engineering has been one important tool in the development of drought-tolerant cultivars. A widely used strategy is the fusion of genes that confer tolerance under the control of the CaMV35S constitutive promoter; however, stress-responsive promoters would constitute the best alternative to the generation of drought-tolerant crops. We characterized the promoter of α-galactosidase soybean (GlymaGAL) gene that was previously identified as highly up-regulated by drought stress...
March 27, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/28323303/the-third-of-a-series-of-articles-for-the-60th-anniversary-of-the-brazilian-society-of-genetics
#7
EDITORIAL
Francisco Mauro Salzano, Fabrício Rodrigues Dos Santos, Klaus Hartfelder, Carlos F M Menck
No abstract text is available yet for this article.
March 20, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/28323302/vegetable-and-fruit-juice-enhances-antioxidant-capacity-and-regulates-antioxidant-gene-expression-in-rat-liver-brain-and-colon
#8
Linhong Yuan, Jinmeng Liu, Jie Zhen, Yao Xu, Shuying Chen, Nicholas Van Halm-Lutterodt, Rong Xiao
To explore the effect of fruit and vegetable (FV) juice on biomarkers of oxidative damage and antioxidant gene expression in rats, 36 adult male Wistar rats were randomly divided into control, low FV juice dosage or high FV juice dosage treatment groups. The rats were given freshly extracted FV juice or the same volume of saline water daily for five weeks. After intervention, serum and tissues specimens were collected for biomarker and gene expression measurement. FV juice intervention increased total antioxidant capacity, glutathione, vitamin C, β-carotene, total polyphenols, flavonoids levels andglutathione peroxidaseenzyme activity in rat serum or tissues (p < 0...
March 20, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/28323301/genetic-variation-of-the-bronze-locus-mc1r-in-turkeys-from-southern-brazil
#9
Josmael Corso, Diego Hepp, Mônica C Ledur, Jane O Peixoto, Nelson J R Fagundes, Thales R O Freitas
Domestic turkeys present several color phenotypes controlled by at least five genetic loci, but only one of these has been identified precisely: the bronze locus, which turned out to be the melanocortin-1 receptor (MC1R) gene. MC1R variation is important for breeders interested in maintaining or developing different color varieties. In this study, we sequenced most of the MC1R gene from 16 White Holland (the main commercial turkey variety) and 19 pigmented turkeys from southern Brazil with two purposes. The first was to describe the MC1R diversity in White Holland turkeys, which may serve as reservoirs of genetic diversity at this locus...
March 20, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/28323300/a-walk-on-the-wild-side-oryza-species-as-source-for-rice-abiotic-stress-tolerance
#10
Paloma Koprovski Menguer, Raul Antonio Sperotto, Felipe Klein Ricachenevsky
Oryza sativa, the common cultivated rice, is one of the most important crops for human consumption, but production is increasingly threatened by abiotic stresses. Although many efforts have resulted in breeding rice cultivars that are relatively tolerant to their local environments, climate changes and population increase are expected to soon call for new, fast generation of stress tolerant rice germplasm, and current within-species rice diversity might not be enough to overcome such needs. The Oryza genus contains other 23 wild species, with only Oryza glaberrima being also domesticated...
March 20, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/28323299/cyanobacterial-nitrogenases-phylogenetic-diversity-regulation-and-functional-predictions
#11
Alberto A Esteves-Ferreira, João Henrique Frota Cavalcanti, Marcelo Gomes Marçal Vieira Vaz, Luna V Alvarenga, Adriano Nunes-Nesi, Wagner L Araújo
Cyanobacteria is a remarkable group of prokaryotic photosynthetic microorganisms, with several genera capable of fixing atmospheric nitrogen (N2) and presenting a wide range of morphologies. Although the nitrogenase complex is not present in all cyanobacterial taxa, it is spread across several cyanobacterial strains. The nitrogenase complex has also a high theoretical potential for biofuel production, since H2 is a by-product produced during N2 fixation. In this review we discuss the significance of a relatively wide variety of cell morphologies and metabolic strategies that allow spatial and temporal separation of N2 fixation from photosynthesis in cyanobacteria...
March 20, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/28304074/relative-frequency-and-estimated-minimal-frequency-of-lysosomal-storage-diseases-in-brazil-report-from-a-reference-laboratory
#12
Roberto Giugliani, Andressa Federhen, Kristiane Michelin-Tirelli, Mariluce Riegel, Maira Burin
Lysosomal storage diseases (LSDs) comprise a heterogeneous group of more than 50 genetic conditions of inborn errors of metabolism (IEM) caused by a defect in lysosomal function. Although there are screening tests for some of these conditions, diagnosis usually depends on specific enzyme assays, which are only available in a few laboratories around the world. A pioneer facility for the diagnosis of IEM and LSDs was established in the South of Brazil in 1982 and has served as a reference service since then. Over the past 34 years, samples from 72,797 patients were referred for investigation of IEM, and 3,211 were confirmed as having an LSD (4...
March 16, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/28304073/the-plant-cell-cycle-pre-replication-complex-formation-and-controls
#13
Juliana Nogueira Brasil, Carinne N Monteiro Costa, Luiz Mors Cabral, Paulo C G Ferreira, Adriana S Hemerly
The multiplication of cells in all living organisms requires a tight regulation of DNA replication. Several mechanisms take place to ensure that the DNA is replicated faithfully and just once per cell cycle in order to originate through mitoses two new daughter cells that contain exactly the same information from the previous one. A key control mechanism that occurs before cells enter S phase is the formation of a pre-replication complex (pre-RC) that is assembled at replication origins by the sequential association of the origin recognition complex, followed by Cdt1, Cdc6 and finally MCMs, licensing DNA to start replication...
March 16, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/28304072/dealing-with-iron-metabolism-in-rice-from-breeding-for-stress-tolerance-to-biofortification
#14
Railson Schreinert Dos Santos, Artur Teixeira de Araujo, Camila Pegoraro, Antonio Costa de Oliveira
Iron is a well-known metal. Used by humankind since ancient times in many different ways, this element is present in all living organisms, where, unfortunately, it represents a two-way problem. Being an essential block in the composition of different proteins and metabolic pathways, iron is a vital component for animals and plants. That is why iron deficiency has a severe impact on the lives of different organisms, including humans, becoming a major concern, especially in developing countries where access to adequate nutrition is still difficult...
March 16, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/28304071/erratum-screening-for-germline-brca1-brca2-tp53-and-chek2-mutations-in-families-at-risk-for-hereditary-breast-cancer-identified-in-a-population-based-study-from-southern-brazil
#15
(no author information available yet)
[This corrects the article doi: 10.1590/1678-4685-GMB-2014-0363].
March 16, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/28266684/dna-methylation-modulates-h19-and-igf2-expression-in-porcine-female-eye
#16
Dongxu Wang, Guodong Wang, Hao Yang, Haibo Liu, Cuie Li, Xiaolan Li, Chao Lin, Yuning Song, Zhanjun Li, Dianfeng Liu
The sexually dimorphic expression of H19/IGF2 is evolutionarily conserved. To investigate whether the expression of H19/IGF2 in the female porcine eye is sex-dependent, gene expression and methylation status were evaluated using quantitative real-time PCR (qPCR) and bisulfite sequencing PCR (BSP). We hypothesized that H19/IGF2 might exhibit a different DNA methylation status in the female eye. In order to evaluate our hypothesis, parthenogenetic (PA) cells were used for analysis by qPCR and BSP. Our results showed that H19 and IGF2 were over-expressed in the female eye compared with the male eye (3-fold and 2-fold, respectively)...
March 6, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/28257527/epigenetic-modifications-and-their-relation-to-caste-and-sex-determination-and-adult-division-of-labor-in-the-stingless-bee-melipona-scutellaris
#17
Carlos A M Cardoso-Júnior, Patrícia Tieme Fujimura, Célio Dias Santos-Júnior, Naiara Araújo Borges, Carlos Ueira-Vieira, Klaus Hartfelder, Luiz Ricardo Goulart, Ana Maria Bonetti
Stingless bees of the genus Melipona, have long been considered an enigmatic case among social insects for their mode of caste determination, where in addition to larval food type and quantity, the genotype also has a saying, as proposed over 50 years ago by Warwick E. Kerr. Several attempts have since tried to test his Mendelian two-loci/two-alleles segregation hypothesis, but only recently a single gene crucial for sex determination in bees was evidenced to be sex-specifically spliced and also caste-specifically expressed in a Melipona species...
March 2, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/28257526/molecular-cloning-characterization-and-expression-analysis-of-a-novel-pdrg1-gene-from-black-tiger-shrimp-penaeus-monodon
#18
Chao Zhao, Wenting Dai, Lihua Qiu
P53 And DNA Damage-Regulated Gene 1 (PDRG1) is a novel gene which plays an important role in chaperone-mediated protein folding. In the present study, the full-length complementary DNA (cDNA) sequence of the PDRG1 gene from Penaeus monodon (PmPDRG1) was cloned by the rapid amplification of cDNA ends (RACE) method. The cDNA of PmPDRG1 spans 1,613 bp, interrupted by only one short intron, and encodes a protein of 136 amino acids with calculated molecular weight of 15.49 kDa. The temporal expression profile of PmPDRG1 in different tissues and in different developmental stages of the ovary was investigated by real-time quantitative PCR (RT-qPCR)...
March 2, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/28257525/foxp-in-tetrapoda-intrinsically-disordered-regions-short-linear-motifs-and-their-evolutionary-significance
#19
Lucas Henriques Viscardi, Luciana Tovo-Rodrigues, Pamela Paré, Nelson Jurandi Rosa Fagundes, Francisco Mauro Salzano, Vanessa Rodrigues Paixão-Côrtes, Claiton Henrique Dotto Bau, Maria Cátira Bortolini
The FOXP subfamily is probably the most extensively characterized subfamily of the forkhead superfamily, playing important roles in development and homeostasis in vertebrates. Intrinsically disorder protein regions (IDRs) are protein segments that exhibit multiple physical interactions and play critical roles in various biological processes, including regulation and signaling. IDRs in proteins may play an important role in the evolvability of genetic systems. In this study, we analyzed 77 orthologous FOXP genes/proteins from Tetrapoda, regarding protein disorder content and evolutionary rate...
March 2, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/28257524/effect-of-soybean-ureases-on-seed-germination-and-plant-development
#20
Ciliana Rechenmacher, Beatriz Wiebke-Strohm, Luisa A de Oliveira-Busatto, Joseph C Polacco, Célia R Carlini, Maria H Bodanese-Zanettini
Urease catalyzes the hydrolysis of urea to ammonia and carbon dioxide. The ammonia (nitrogen (N) product of urease activity) is incorporated into organic compounds. Thus, urease is involved in N remobilization, as well as in primary N assimilation. Two urease isoforms have been described for soybean: the embryo-specific, encoded by the Eu1 gene, and the ubiquitous urease, encoded by Eu4. A third urease-encoding gene was recently identified, designated Eu5, which encodes the putative protein product SBU-III...
March 2, 2017: Genetics and Molecular Biology
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