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Genetics and Molecular Biology

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https://www.readbyqxmd.com/read/27801483/molecular-data-highlight-hybridization-in-squirrel-monkeys-saimiri-cebidae
#1
Jeferson Carneiro, Luis Fernando da Silva Rodrigues-Filho, Horacio Schneider, Iracilda Sampaio
Hybridization has been reported increasingly frequently in recent years, fueling the debate on its role in the evolutionary history of species. Some studies have shown that hybridization is very common in captive New World primates, and hybrid offspring have phenotypes and physiological responses distinct from those of the "pure" parents, due to gene introgression. Here we used the TA15 Alu insertion to investigate hybridization in the genus Saimiri. Our results indicate the hybridization of Saimiri boliviensis peruviensis with S...
October 31, 2016: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/27801481/genotoxic-and-mutagenic-effects-of-polluted-surface-water-in-the-midwestern-region-of-brazil-using-animal-and-plant-bioassays
#2
Priscila Leocádia Rosa Dourado, Monyque Palagano da Rocha, Liriana Mara Roveda, Jorge Luiz Raposo, Liliam Sílvia Cândido, Claudia Andréa Lima Cardoso, Maria Aparecida Marin Morales, Kelly Mari Pires de Oliveira, Alexeia Barufatti Grisolia
This study aimed to evaluate DNA damage in animal and plant cells exposed to water from the Água Boa stream (Dourados, Mato Grosso do Sul, Brazil) by using bioassays, and to identify the chemical compounds in the water to determine the water quality in the area. Through the cytotoxicity bioassay with Allium cepa, using micronucleus test, and comet assay, using Astyanax altiparanae fish, the results indicated that biological samples were genetically altered. Micronuclei were observed in erythrocytes of A. altiparanae after exposure to water from locations close to industrial waste discharge...
October 31, 2016: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/27768156/contribution-of-wuschel-related-homeobox-wox-genes-to-identify-the-phylogenetic-relationships-among-petunia-species
#3
Ana Lúcia Anversa Segatto, Claudia Elizabeth Thompson, Loreta Brandão Freitas
Developmental genes are believed to contribute to major changes during plant evolution, from infrageneric to higher levels. Due to their putative high sequence conservation, developmental genes are rarely used as molecular markers, and few studies including these sequences at low taxonomic levels exist. WUSCHEL-related homeobox genes (WOX) are transcription factors exclusively present in plants and are involved in developmental processes. In this study, we characterized the infrageneric genetic variation of Petunia WOX genes...
October 20, 2016: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/27768155/gene-expression-analysis-reveals-important-pathways-for-drought-response-in-leaves-and-roots-of-a-wheat-cultivar-adapted-to-rainfed-cropping-in-the-cerrado-biome
#4
Liane Balvedi Poersch-Bortolon, Jorge Fernando Pereira, Antonio Nhani, Hebert Hernán Soto Gonzáles, Gisele Abigail Montan Torres, Luciano Consoli, Rafael Augusto Arenhart, Maria Helena Bodanese-Zanettini, Márcia Margis-Pinheiro
Drought limits wheat production in the Brazilian Cerrado biome. In order to search for candidate genes associated to the response to water deficit, we analyzed the gene expression profiles, under severe drought stress, in roots and leaves of the cultivar MGS1 Aliança, a well-adapted cultivar to the Cerrado. A set of 4,422 candidate genes was found in roots and leaves. The number of down-regulated transcripts in roots was higher than the up-regulated transcripts, while the opposite occurred in leaves. The number of common transcripts between the two tissues was 1,249, while 2,124 were specific to roots and 1,049 specific to leaves...
October 20, 2016: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/27768154/investigator-argus-x-12-study-on-the-population-of-northern-croatia
#5
Josip Crnjac, Petar Ozretić, Siniša Merkaš, Martina Ratko, Mateja Lozančić, Marina Korolija, Maja Popović, Gordan Mršić
X chromosome STR typing has emerged recently as a powerful tool, complementary to autosomal STR typing, in solving complex forensic and missing person cases. Investigator® Argus X-12 is a commercial product that allows co-amplification of 12 X chromosomal markers belonging to four linkage groups (LGs). In this study, we analyzed by capillary electrophoresis blood samples from 100 females and 102 males from a population of northern Croatia. Statistical analysis included calculation of allele and haplotype frequencies, as well as forensic parameters...
October 20, 2016: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/27768153/sequencing-and-de-novo-assembly-of-the-asian-gypsy-moth-transcriptome-using-the-illumina-platform
#6
Fan Xiaojun, Yang Chun, Liu Jianhong, Zhang Chang, Li Yao
The Asian gypsy moth (Lymantria dispar) is a serious pest of forest and shade trees in many Asian and some European countries. However, there have been few studies of L. dispar genetic information and comprehensive genetic analyses of this species are needed in order to understand its genetic and metabolic sensitivities, such as the molting mechanism during larval development. In this study, high-throughput sequencing technology was used to sequence the transcriptome of the Asian subspecies of the gyspy moth, after which a comprehensive analysis of chitin metabolism was undertaken...
October 20, 2016: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/27727362/the-diversity-of-citrus-endophytic-bacteria-and-their-interactions-with-xylella-fastidiosa-and-host-plants
#7
João Lúcio Azevedo, Welington Luiz Araújo, Paulo Teixeira Lacava
The bacterium Xylella fastidiosa is the causal agent of citrus variegated chlorosis (CVC) and has been associated with important losses in commercial orchards of all sweet orange [Citrus sinensis (L.)] cultivars. The development of this disease depends on the environmental conditions, including the endophytic microbial community associated with the host plant. Previous studies have shown that X. fastidiosa interacts with the endophytic community in xylem vessels as well as in the insect vector, resulting in a lower bacterial population and reduced CVC symptoms...
October 10, 2016: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/27727361/the-histone-genes-cluster-in-rhynchosciara-americana-and-its-transcription-profile-in-salivary-glands-during-larval-development
#8
Fábio Siviero, Paula Rezende-Teixeira, Alexandre de Andrade, Roberto Vicente Santelli, Glaucia Maria Machado-Santelli
In this work we report the characterization of the Rhynchosciara americana histone genes cluster nucleotide sequence. It spans 5,131 bp and contains the four core histones and the linker histone H1. Putative control elements were detected. We also determined the copy number of the tandem repeat unit through quantitative PCR, as well as the unequivocal chromosome location of this unique locus in chromosome A band 13. The data were compared with histone clusters from the genus Drosophila, which are the closest known homologues...
October 10, 2016: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/27727359/genetic-frequencies-related-to-severe-or-profound-sensorineural-hearing-loss-in-inner-mongolia-autonomous-region
#9
Yongzhi Liu, Liying Ao, Haitao Ding, Dongli Zhang
The aim was to study the frequencies of common deafness-related mutations and their contribution to hearing loss in different regions of Inner Mongolia. A total of 738 deaf children were recruited from five different ethnic groups of Inner Mongolia, including Han Chinese (n=486), Mongolian (n=216), Manchurian (n=24), Hui (n=6) and Daur (n=6). Nine common mutations in four genes (GJB2, SLC26A4, GJB3 and mitochondrial MT-RNR1 gene) were detected by allele-specific PCR and universal array. At least one mutated allele was detected in 282 patients...
October 10, 2016: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/27706371/the-effects-of-old-and-recent-migration-waves-in-the-distribution-of-hbb-s-globin-gene-haplotypes
#10
Juliana D Lindenau, Sandrine C Wagner, Simone M de Castro, Mara H Hutz
Sickle cell hemoglobin is the result of a mutation at the sixth amino acid position of the beta (β) globin chain. The HBB*S gene is in linkage disequilibrium with five main haplotypes in the β-globin-like gene cluster named according to their ethnic and geographic origins: Bantu (CAR), Benin (BEN), Senegal (SEN), Cameroon (CAM) and Arabian-Indian (ARAB). These haplotypes demonstrated that the sickle cell mutation arose independently at least five times in human history. The distribution of βS haplotypes among Brazilian populations showed a predominance of the CAR haplotype...
October 3, 2016: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/27706370/diversity-and-evolution-of-plant-diacylglycerol-acyltransferase-dgats-unveiled-by-phylogenetic-gene-structure-and-expression-analyses
#11
Andreia Carina Turchetto-Zolet, Ana Paula Christoff, Franceli Rodrigues Kulcheski, Guilherme Loss-Morais, Rogerio Margis, Marcia Margis-Pinheiro
Since the first diacylglycerol acyltransferase (DGAT) gene was characterized in plants, a number of studies have focused on understanding the role of DGAT activity in plant triacylglycerol (TAG) biosynthesis. DGAT enzyme is essential in controlling TAGs synthesis and is encoded by different genes. DGAT1 and DGAT2 are the two major types of DGATs and have been well characterized in many plants. On the other hand, the DGAT3 and WS/DGAT have received less attention. In this study, we present the first general view of the presence of putative DGAT3 and WS/DGAT in several plant species and report on the diversity and evolution of these genes and its relationships with the two main DGAT genes (DGAT1 and DGAT2)...
October 3, 2016: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/27648768/wolbachia-in-guilds-of-anastrepha-fruit-flies-tephritidae-and-parasitoid-wasps-braconidae
#12
Rodrigo O Mascarenhas, Leandro F Prezotto, André Luiz P Perondini, Celso Luiz Marino, Denise Selivon
The endosymbiont Wolbachia is efficiently transmitted from females to their progenies, but horizontal transmission between different taxa is also known to occur. Aiming to determine if horizontal transmission might have occurred between Anastrepha fruit flies and associated braconid wasps, infection by Wolbachia was screened by amplification of a fragment of the wsp gene. Eight species of the genus Anastrepha were analyzed, from which six species of associated parasitoid wasps were recovered. The endosymbiont was found in seven Anastrepha species and in five species of braconids...
September 19, 2016: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/27648767/the-rearranged-mitochondrial-genome-of-leptopilina-boulardi-hymenoptera-figitidae-a-parasitoid-wasp-of-drosophila
#13
Daniel S Oliveira, Tiago M F F Gomes, Elgion L S Loreto
The partial mitochondrial genome sequence of Leptopilina boulardi (Hymenoptera: Figitidae) was characterized. Illumina sequencing was used yielding 35,999,679 reads, from which 102,482 were utilized in the assembly. The length of the sequenced region of this partial mitochondrial genome is 15,417 bp, consisting of 13 protein-coding, two rRNA, and 21tRNA genes (the trnaM failed to be sequenced) and a partial A+T-rich region. All protein-coding genes start with ATN codons. Eleven protein-coding genes presented TAA stop codons, whereas ND6 and COII that presented TA, and T nucleotides, respectively...
September 19, 2016: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/27648766/the-complete-mitochondrial-genome-of-the-threatened-neotropical-catfish-lophiosilurus-alexandri-silurifomes-pseudopimelodidae-and-phylogenomic-analysis-indicate-monophyly-of-pimelodoidea
#14
Daniel Cardoso Carvalho, Violeta da Rocha Perini, Alex Schomaker Bastos, Igor Rodrigues da Costa, Ronald Kennedy Luz, Carolina Furtado, Francisco Prosdocimi
Lophiosilurus alexandri is an endemic catfish from the São Francisco River Basin (Brazil) popularly known as pacamã, which has economic potential for aquaculture farming. The mitochondrial genome was sequenced for the threatened Neotropical catfish L. alexandri. Assembly into scaffolds using MIRA and MITObim software produced the whole, circularized mitochondrial genome, which comprises 16,445 bp and presents the typical gene arrangement of Teleostei mitochondria. A phylogenomic analysis was performed after the concatenation of all proteins obtained from whole mitogenomes of 20 Siluriformes and two outgroups...
September 19, 2016: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/27898126/the-second-of-a-series-of-articles-for-the-60th-anniversary-of-the-brazilian-society-of-genetics
#15
EDITORIAL
Francisco Mauro Salzano, Fabrício Rodrigues Dos Santos, Klaus Hartfelder, Carlos F M Menck
No abstract text is available yet for this article.
October 2016: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/27801482/expression-of-myogenes-in-longissimus-dorsi-muscle-during-prenatal-development-in-commercial-and-local-piau-pigs
#16
Evelyze Pinheiro Dos Reis, Débora Martins Paixão, Otávio José Bernardes Brustolini, Fabyano Fonseca E Silva, Walmir Silva, Flávio Marcos Gomes de Araújo, Anna Christina de Matos Salim, Guilherme Oliveira, Simone Eliza Facioni Guimarães
This study used qRT-PCR to examine variation in the expression of 13 myogenes during muscle development in four prenatal periods (21, 40, 70 and 90 days post-insemination) in commercial (the three-way Duroc, Landrace and Large-White cross) and local Piau pig breeds that differ in muscle mass. There was no variation in the expression of the CHD8, EID2B, HIF1AN, IKBKB, RSPO3, SOX7 and SUFU genes at the various prenatal ages or between breeds. The MAP2K1 and RBM24 genes showed similar expression between commercial and Piau pigs but greater expression (p < 0...
October 2016: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/27791217/agmatoploidy-and-symploidy-a-critical-review
#17
Marcelo Guerra
Agmatoploidy is a type of chromosome rearrangement that involves the fragmentation of an entire chromosome complement, generating a diploid with double its original chromosome number. Agmatoploidy and other related karyotype changes, such as symploidy (the opposite change, promoted by chromosome fusion), partial agmatoploidy, polyagmatoploidy, etc., are restricted to species with holokinetic chromosomes and are assumed to play an important role in their karyotype evolution. However, a critical review of the literature shows that examples of chromosome number doubling by agmatoploidy are rare and not clearly demonstrated, while partial agmatoploidy and partial symploidy seem to be the same as ascending and descending disploidy, respectively...
October 2016: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/27727360/association-between-interleukin-6-174-g-c-promoter-gene-polymorphism-and-runners-responses-to-the-dietary-ingestion-of-antioxidant-supplementation-based-on-pequi-caryocar-brasiliense-camb-oil-a-before-after-study
#18
Ana Luisa Miranda-Vilela, Ieler Ferreira Ribeiro, Cesar Koppe Grisolia
Exercise is a double-edged sword: when practiced in moderation, it increases the expression of antioxidant enzymes, but when practiced strenuously it causes oxidative stress and cell damage. In this context, polymorphisms in the interleukin (IL)-6 gene should be investigated better because they can influence performance, at least in exercise that generates oxidative stress and leads to muscular injuries with consequent inflammation. In this work, we investigated the influence of IL-6 -174 G/C polymorphism on tissue damage and inflammation markers, lipid peroxidation, hemogram and lipid profile of runners before and after ingestion of 400 mg of pequi oil in capsules supplied daily for 14 consecutive days...
October 2016: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/27505307/oxytocin-and-arginine-vasopressin-receptor-evolution-implications-for-adaptive-novelties-in-placental-mammals
#19
Pamela Paré, Vanessa R Paixão-Côrtes, Luciana Tovo-Rodrigues, Pedro Vargas-Pinilla, Lucas Henriques Viscardi, Francisco Mauro Salzano, Luiz E Henkes, Maria Catira Bortolini
Oxytocin receptor (OXTR) and arginine vasopressin receptors (AVPR1a, AVPR1b, and AVPR2) are paralogous genes that emerged through duplication events; along the evolutionary timeline, owing to speciation, numerous orthologues emerged as well. In order to elucidate the evolutionary forces that shaped these four genes in placental mammals and to reveal specific aspects of their protein structures, 35 species were selected. Specifically, we investigated their molecular evolutionary history and intrinsic protein disorder content, and identified the presence of short linear interaction motifs...
August 8, 2016: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/27561110/familial-dysautonomia-mechanisms-and-models
#20
Paula Dietrich, Ioannis Dragatsis
Hereditary Sensory and Autonomic Neuropathies (HSANs) compose a heterogeneous group of genetic disorders characterized by sensory and autonomic dysfunctions. Familial Dysautonomia (FD), also known as HSAN III, is an autosomal recessive disorder that affects 1/3,600 live births in the Ashkenazi Jewish population. The major features of the disease are already present at birth and are attributed to abnormal development and progressive degeneration of the sensory and autonomic nervous systems. Despite clinical interventions, the disease is inevitably fatal...
August 4, 2016: Genetics and Molecular Biology
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