journal
MENU ▼
Read by QxMD icon Read
search

Cells, Tissues, Organs

journal
https://www.readbyqxmd.com/read/29949808/simultaneous-study-of-mechanical-stretch-induced-cell-proliferation-and-apoptosis-on-c2c12-myoblasts
#1
Yu Feng, Xiang-Yang Tian, Peng Sun, Ze-Peng Cheng, Reng-Fei Shi
Mechanical stretch may cause myoblasts to either proliferate or undergo apoptosis. Identifying the molecular events that switch the fate of a stretched cell from proliferation to apoptosis is practically important in the field of regenerative medicine. A recent study on vascular smooth muscle cells illustrated that identification of these events may be achieved by addressing the stretch-induced opposite cellular outcomes simultaneously within a single investigation. To define conditions or a model in which both proliferation and apoptosis can be studied at the same time, we exposed in vitro cultured C2C12 myoblasts to a cyclic mechanical stretch regimen of 15% elongation at a stretching frequency of 1 Hz for 0, 2, 4, 6, or 8 h every day, consecutively, for 3 days...
June 27, 2018: Cells, Tissues, Organs
https://www.readbyqxmd.com/read/29949803/comparison-of-preterm-and-term-wharton-s-jelly-derived-mesenchymal-stem-cell-properties-in-different-oxygen-tensions
#2
Saloni Balgi-Agarwal, Caitlyn Winter, Alexis Corral, Shamimunisa B Mustafa, Peter Hornsby, Alvaro Moreira
Mesenchymal stem cells (MSCs) have shown promise as therapeutic agents in treating morbidities associated with premature birth. MSCs derived from the human umbilical cord are easy to isolate and have low immunogenicity and a robust ability to secrete paracrine factors. To date, there are no studies evaluating preterm versus term umbilical cord tissue-derived MSCs. Therefore, our aim was twofold: (1) to compare stem cell properties in preterm versus term MSCs and (2) to examine the impact of oxygen tension on stem cell behavior...
June 27, 2018: Cells, Tissues, Organs
https://www.readbyqxmd.com/read/29949800/distribution-of-collagen-i-iii-and-iv-and-laminin-in-the-human-liver-during-prenatal-development
#3
Marko Jović, Ivan Nikolić, Vera Todorović, Aleksandar Petrović, Vladimir Petrović, Marijola Mojsilović, Tijana Denčić
In the absence of systematized data on the extracellular matrix components during prenatal liver development, the present study aimed to investigate the time of appearance and distribution of collagen types I, III, and IV and laminin. The study material included embryonic and fetal livers, aged 7-37 weeks, categorized into 3 trimesters. The material was stained using hematoxylin-eosin and immunohistochemistry methods for the identification of collagen I, III, and IV and laminin. Collagen I was detected near the end of the first trimester in the capsules and walls of interlobular veins...
June 27, 2018: Cells, Tissues, Organs
https://www.readbyqxmd.com/read/29925059/morphological-characterization-of-basally-located-uninucleate-trophoblast-cells-as-precursors-of-bovine-binucleate-trophoblast-giant-cells
#4
Jeannette Attiger, Alois Boos, Karl Klisch
Binucleate trophoblast giant cells (TGCs) are one characteristic feature of the ruminant placenta. In cows, the frequency of TGCs remains constant for most of the duration of pregnancy. As TGCs are depleted by their fusion with uterine epithelial cells, they need to be constantly formed. It is still unclear whether they develop from stem cells within the trophectoderm or whether they can arise from any uninucleate trophoblast cell (UTC). Within the latter, generally accepted theory, a basally located uninucleate cell (BUC) without contact to the feto-maternal interface would represent a transient cell between a UTC and a TGC...
June 20, 2018: Cells, Tissues, Organs
https://www.readbyqxmd.com/read/29913455/grape-leucoanthocyanidin-protects-liver-tissue-in-albino-rabbits-with-nonalcoholic-hepatic-steatosis
#5
Reginaldo Franklin, Rodrigo Freitas Monte Bispo, Célio Fernando Sousa-Rodrigues, Lucas Alves Sarmento Pires, Albino Fonseca, Marcio Antonio Babinski
Nonalcoholic fatty liver disease (NAFLD) is a common ailment. It is usually found in association with diabetes or obesity. There are no approved drugs to treat this condition. The study of flavonoid consumption has increased over the decades due to their antioxidative properties, although the literature is scarce when it comes to their effects in liver tissue. The purpose of this study was to evaluate the role of leucoanthocyanidin in nonalcoholic hepatic steatosis. Thirty male albino rabbits were divided in 3 groups...
June 18, 2018: Cells, Tissues, Organs
https://www.readbyqxmd.com/read/29742511/decellularized-pancreas-matrix-scaffolds-for-tissue-engineering-using-ductal-or-arterial-catheterization
#6
Javad Hashemi, Parvin Pasalar, Masoud Soleimani, Ehsan Arefian, Reza Khorramirouz, Aram Akbarzadeh, Fariba Ghorbani, Seyed Ehsan Enderami, Abdol Mohammad Kajbafzadeh
INTRODUCTION: Diabetes is known as a worldwide disease with a great burden on society. Since therapeutic options cover a limited number of target points, new therapeutic strategies in the field of regenerative medicine are considered. Bioscaffolds along with islet cells would provide bioengineered tissue as a substitute for β-cells. The perfusion-decellularization technique is considered to create such scaffolds since they mimic the compositional, architectural, and biomechanical nature of a native organ...
May 9, 2018: Cells, Tissues, Organs
https://www.readbyqxmd.com/read/29734141/innervation-of-the-human-incisive-papilla-comparison-with-other-oral-regions
#7
Chiaki Endo, Tadasu Sato, Takehiro Yajima, Kaoru Igarashi, Hiroyuki Ichikawa
Immunohistochemistry for several neurochemical substances was performed on the human incisive papilla and other oral structures. Sodium channel alpha subunit 7 (SCN7A) protein-immunoreactive (IR) Schwann cells and protein gene product 9.5 (PGP 9.5)-IR nerve fibers made nerve plexuses beneath the epithelium of the palate, including the incisive papilla, tongue, and lip. SCN7A immunoreactivity could also be detected in lamellated and nonlamellated capsules of corpuscle endings. Lamellated SCN7A-IR corpuscle endings were mostly restricted to the mucous and cutaneous lips...
May 7, 2018: Cells, Tissues, Organs
https://www.readbyqxmd.com/read/29672316/immunolocalization-of-receptor-and-chemoreceptor-modules-in-the-sheep-vomeronasal-organ
#8
Dalia Ibrahim
The vomeronasal organ (VNO) is the peripheral receptor organ of the accessory olfactory system, which is responsible for both sexual and innate behaviors. The degree of neuronal differentiation and maturation of the vomeronasal receptor cells together with the verification of the presence of the solitary chemoreceptor cells (SCCs) in the VNO of Corriedale sheep were assessed using immunofluorescence. A protein gene product 9.5 (PGP 9.5), which is a neuronal marker recognized to be expressed in most neurons of vertebrate species, an olfactory marker protein (OMP) that is precise for mature olfactory receptor cells, and lastly phospholipase C-β2 (PLC-β2), a marker in the signal transduction pathway of SCCs, were all tested...
April 19, 2018: Cells, Tissues, Organs
https://www.readbyqxmd.com/read/29550820/the-role-of-epithelial-stat3-in-amelogenesis-during-mouse-incisor-renewal
#9
Bin Zhang, Bo Meng, Edward Viloria, Adrien Naveau, Bernhard Ganss, Andrew H Jheon
The aim of this study was to evaluate the role of epithelial signal transducer and activator of transcription 3 (STAT3) in mouse incisor amelogenesis. Since Stat3 is expressed in the epithelial component of developing and adult mouse teeth, we generated and analyzed Krt14Cre/+;Stat3fl/fl mutant mice in which Stat3 was inactivated in epithelia including ameloblast progenitors and ameloblasts, the cells responsible for enamel formation. Histological analysis showed little enamel matrix in mutant incisors compared to controls...
March 16, 2018: Cells, Tissues, Organs
https://www.readbyqxmd.com/read/29913446/segmentation-of-dilated-hemorrhoidal-veins-in-hemorrhoidal-disease
#10
Lucio Díaz-Flores, Ricardo Gutiérrez, Miriam González-Gómez, Pino García, Francisco J Sáez, Lucio Díaz-Flores, José Luis Carrasco, Juan F Madrid
Vein segmentation is a vascular remodeling process mainly studied in experimental conditions and linked to hemodynamic factors, with clinical implications. The aim of this work is to assess the morphologic characteristics, associated findings, and mechanisms that participate in vein segmentation in humans. To this end, we examined 156 surgically obtained cases of hemorrhoidal disease. Segmentation occurred in 65 and was most prominent in 15, which were selected for serial sections, immunohistochemistry, and immunofluorescence procedures...
2018: Cells, Tissues, Organs
https://www.readbyqxmd.com/read/29843137/reactive-astrogliosis-in-an-experimental-model-of-fibromyalgia-effect-of-dexmedetomidine
#11
Rasha B Abd-Ellatief, Heba K Mohamed, Hassan I Kotb
To our knowledge, this is the first study which investigates the induction of neuroinflammation in rats using an acidic-saline model of fibromyalgia. It is well known that the hippocampus has a fundamental role in pain perception, and astrocytes play a crucial role in pain signaling. Our aim is to evaluate the ability of dexmedetomidine to attenuate the inflammatory responses induced in astrocytes. In a group of healthy rats, induction of chronic muscle pain by intramuscular injection of 100 µL of acidic saline on days 0 and 5 resulted in peripheral sensitization (measured using the von Frey test) and significant (p < 0...
2018: Cells, Tissues, Organs
https://www.readbyqxmd.com/read/29495007/functional-morphology-of-thecal-glands-in-the-ovary-of-japanese-quails-coturnix-japonica
#12
Daniela Rodler, Fred Sinowatz
The role of thecal glands in the ovary of birds remains controversial. Using transmission electron microscopy and immunohistochemistry, immunohistochemical localisation of cyclooxygenase I and II (COX-1 and COX-2), oestrogen receptor α and β (ER-α and ER-β), androgen receptor (AR) and progesterone receptor (PR), a detailed analysis of the thecal glands was performed. Our ultrastructural studies revealed that the thecal glands of the quail ovary consist of 2 cell types, steroid-producing cells (SPCs) and enclosing cells (ENCs)...
2018: Cells, Tissues, Organs
https://www.readbyqxmd.com/read/29414820/osteoconduction-of-unrestricted-somatic-stem-cells-on-an-electrospun-polylactic-co-glycolic-acid-scaffold-coated-with-nanohydroxyapatite
#13
Maryam Shahi, Mahmood Nadari, Mehdi Sahmani, Ehsan Seyedjafari, Naser Ahmadbeigi, Amir Peymani
The limitation of traditional bone grafts could be overcome by applying engineered bone constructs, which are mainly produced by seeding suitable stem cells on appropriate scaffolds. So far, bone marrow-derived stromal cells have been the most applied cells in bone tissue engineering, but current data show that unrestricted somatic stem cells (USSCs) from human cord blood might actually be a better stem cell source due to the accessibility and noninvasive procedure of collection. In this study, we cultured USSCs on a plasma-treated electrospun polylactic-co-glycolic acid (PLGA) scaffold coated with nanohydroxyapatite (nHA)...
2018: Cells, Tissues, Organs
https://www.readbyqxmd.com/read/29414801/ultramorphological-characteristics-of-podocyte-development-in-the-human-fetal-metanephros
#14
Marija Dakovic Bjelakovic, Slobodan Vlajkovic, Milica Bjelakovic
The aim of this study was to determine the developmental characteristics of podocytes in the human fetal metanephros using scanning electron microscopy, light microscopy, and transmission electron microscopy. Kidney samples of 15 human fetuses of both sexes (gestational age 10-22 weeks) were analyzed. At the S-shaped body stage, primitive podocytes were arranged in a layer of cuboidal cells beneath the vascular cleft. When observed from Bowman's space, the demarcation between adjacent podocytes was not clear, but mild depressions indicated cell boundaries...
2018: Cells, Tissues, Organs
https://www.readbyqxmd.com/read/29393196/responsiveness-of-infrapatellar-fat-pad-volume-change-to-body-weight-loss-or-gain-data-from-the-osteoarthritis-initiative
#15
Eva Steidle-Kloc, Torben Dannhauer, Wolfgang Wirth, Felix Eckstein
Obesity is a potent risk factor for knee osteoarthritis (OA) that is driven by mechanical and potentially endocrine mechanisms, and it affects women more frequently than men. The infrapatellar fat pat (IPFP) represents a potential link between obesity, intra-articular inflammation and structural pathology. Here we investigate whether the IPFP is responsive to body weight loss/gain in women and how its responsiveness to weight change compares to that of subcutaneous fat (SCF) of the thigh. All female participants of the Osteoarthritis Initiative (OAI) with ≥10% weight loss/gain between baseline and a 2-year follow-up were included...
2018: Cells, Tissues, Organs
https://www.readbyqxmd.com/read/29393161/human-embryonic-kidney-293-cells-a-vehicle-for-biopharmaceutical-manufacturing-structural-biology-and-electrophysiology
#16
Jianwen Hu, Jizhong Han, Haoran Li, Xian Zhang, Lan Lan Liu, Fei Chen, Bin Zeng
Mammalian cells, e.g., CHO, BHK, HEK293, HT-1080, and NS0 cells, represent important manufacturing platforms in bioengineering. They are widely used for the production of recombinant therapeutic proteins, vaccines, anticancer agents, and other clinically relevant drugs. HEK293 (human embryonic kidney 293) cells and their derived cell lines provide an attractive heterologous system for the development of recombinant proteins or adenovirus productions, not least due to their human-like posttranslational modification of protein molecules to provide the desired biological activity...
2018: Cells, Tissues, Organs
https://www.readbyqxmd.com/read/29393155/porous-chitosan-nano-hydroxyapatite-composite-scaffolds-incorporating-simvastatin-loaded-plga-microspheres-for-bone-repair
#17
Yu Li, Zhanzhao Zhang, Zhiyong Zhang
The combination of bone tissue scaffolds with osteogenic induction factors is an effective strategy to facilitate bone healing processes. Here, chitosan (CS)/nano-hydroxyapatite (HA) scaffolds containing simvastatin (SIM)-loaded PLGA microspheres were fabricated by combining a freeze-drying technique with a modified water-oil-water emulsion method. The CS/HA weight ratio of 1:2 was selected by analyzing the effect of HA content on the micro-architecture, mechanical strength, and biocompatibility of the scaffold...
2018: Cells, Tissues, Organs
https://www.readbyqxmd.com/read/29241215/erratum
#18
(no author information available yet)
No abstract text is available yet for this article.
2017: Cells, Tissues, Organs
https://www.readbyqxmd.com/read/29161703/expression-profile-of-notch3-in-mouse-spermatogonia
#19
Ryu Okada, Megumi Fujimagari, Eri Koya, Yoshikazu Hirose, Tomomi Sato, Yukio Nishina
Stable and sustainable spermatogenesis is supported by the strict regulation of self-renewal and differentiation of spermatogonial stem cells (SSC), which are a rare population of undifferentiated spermatogonia. It has been revealed that some signaling factors regulate the self-renewal of SSC; however, the molecular mechanism of SSC maintenance is still not completely understood. Notch signaling is an evolutionarily conserved juxtacrine signaling that plays important roles in the cell fate determination of various tissue stem cells...
2017: Cells, Tissues, Organs
https://www.readbyqxmd.com/read/29131080/complete-static-repopulation-of-decellularized-porcine-tissues-for-heart-valve-engineering-an-in-vitro-study
#20
Annelies Roosens, Mahtab Asadian, Nathalie De Geyter, Pamela Somers, Ria Cornelissen
To date, a completely in vitro repopulated tissue-engineered heart valve has not been developed. This study focused on sequentially seeding 2 cell populations onto porcine decellularized heart valve leaflets (HVL) and pericardia (PER) to obtain fully repopulated tissues. For repopulation of the interstitium, porcine valvular interstitial cells (VIC) and bone marrow-derived mesenchymal stem cells (BM-MSC) or adipose tissue-derived stem cells (ADSC) were used. In parallel, the culture medium was supplemented with ascorbic acid 2-phosphate (AA) and its effect on recolonization was investigated...
2017: Cells, Tissues, Organs
journal
journal
34564
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"