Read by QxMD icon Read

Cells, Tissues, Organs

Marijana Skific, Mirna Golemovic, Kristina Crkvenac-Gornik, Radovan Vrhovac, Branka Golubic Cepulic
Due to their ability to induce immunological tolerance in the recipient, mesenchymal stromal cells (MSCs) have been utilized in the treatment of various hematological and immune- and inflammation-mediated diseases. The clinical application of MSCs implies prior in vitro expansion that usually includes the use of fetal bovine serum (FBS). The present study evaluated the effect of different platelet lysate (PL) media content on the biological properties of MSCs. MSCs were isolated from the bone marrow of 13 healthy individuals and subsequently expanded in three different culture conditions (10% PL, 5% PL, 10% FBS) during 4 passages...
September 17, 2018: Cells, Tissues, Organs
Fabricio Ivan Busolini, Luis Ezequiel Gallol, Graciela Beatríz Rodríguez, Verónica Palmira Filippa, Fabian Heber Mohamed
The pineal gland of mammals undergoes morphological and biochemical changes throughout the gestation period. In viscachas, a seasonal breeding rodent, pregnancy lasts approximately 154 days and 3 stages can be defined, i.e., early, mid, and late pregnancy. The purpose of this study is to analyze morphometric variations in the expression of S-100 protein, glial fibrillary acidic protein (GFAP), and vimentin in the interstitial cells (IC) in pregnant and nonpregnant viscachas by immunohistochemistry (IHC). We also aim to evaluate a probable relation between glandular activity and pregnancy...
September 13, 2018: Cells, Tissues, Organs
Gustavo de Sá Schiavo Matias, Nathia Nathaly Rigoglio, Ana Claudia Oliveira Carreira, Patricia Romagnolli, Rodrigo da Silva Nunes Barreto, Andrea Maria Mess, Maria Angelica Miglino, Paula Fratini
Due to the scarcity of tissues and organs for transplantation, the demand for bioengineered tissues is increasing with the advancement of technologies and new treatments in human and animal regenerative medicine. Thus, decellularized placental extracellular matrix (ECM) has emerged as a new tool for the production of biological scaffolds for subsequent recellularization and implantation for recovery of injured areas or even for replacement of organ and tissue fractions. To be classified as an ideal biological scaffold, the ECM must be acellular and preserve its proteins and physical features to be useful for cellular adhesion...
September 10, 2018: Cells, Tissues, Organs
Ayşegül Mendi, Beyza Gökçınar Yağcı, Nurdan Saraç, Mustafa Kızıloğlu, Aysel Uğur, Duygu Uçkan, Derviş Yılmaz
The aim of this study is to demonstrate and compare the differentiation, proliferation, migration and inflammatory behavior of dental pulp- and bone marrow-derived mesenchymal stem cells (DP-MSCs and BM-MSCs) in response to a Hypericum perforatum ethanol extract. Using xCELLigence, a real-time monitoring system, a dose of 10 µg/mL was found to be the most efficient concentration for vitality. The IC50 values and doubling time were calculated. The results showed that H. perforatum L. was able to accelerate osteogenic differentiation in DP-MSCs, but calcium granulation was impaired in BM-MSCs...
August 17, 2018: Cells, Tissues, Organs
Alexie A Larson, Brian C Syverud, Shelby E Florida, Brittany L Rodriguez, Molly N Pantelic, Lisa M Larkin
Our lab showed that administration of dexamethasone (DEX) stimulated myogenesis and resulted in advanced structure in our engineered skeletal muscle units (SMU). While administration of 25 nM DEX resulted in the most advanced structure, 10 nM dosing resulted in the greatest force production. We hypothesized that administration of 25 nM DEX during the entire fabrication process was toxic to the cells and that administration of DEX at precise time points during myogenesis would result in SMU with a more advanced structure and function...
August 17, 2018: Cells, Tissues, Organs
Martin Blum, Tim Ott
The function of normal and defective candidate genes for human genetic diseases, which are rapidly being identified in large numbers by human geneticists and the biomedical community at large, will be best studied in relevant and predictive model organisms that allow high-speed verification, analysis of underlying developmental, cellular and molecular mechanisms, and establishment of disease models to test therapeutic options. We describe and discuss the pros and cons of the frog Xenopus, which has been extensively used to uncover developmental mechanisms in the past, but which is being underutilized as a biomedical model...
August 9, 2018: Cells, Tissues, Organs
Yutaka Yamaguchi, Shigehito Yamada
The Kyoto Collection of Human Embryos and Fetuses, the largest collection of human embryos worldwide, was initiated in the 1960s, and the Congenital Anomaly Research Center of Kyoto University was established in 1975 for long-term storage of the collection and for the promotion of research into human embryonic and fetal development. Currently, the Kyoto Collection comprises approximately 45,000 specimens of human embryonic or fetal development and is renowned for the following unique characteristics: (1) the collection is considered to represent the total population of fetal specimens nationwide in Japan, (2) it comprises a large number of specimens with a variety of external malformations, and (3) for most specimens there are clinical and epidemiological data from the mothers and the pregnancies concerned...
July 31, 2018: Cells, Tissues, Organs
Maryam Sadat Tafakh, Massoud Saidijam, Tayebeh Ranjbarnejad, Sara Malih, Solmaz Mirzamohammadi, Rezvan Najafi
BACKGROUND: A high expression of prostaglandin E2 (PGE2) is found in colorectal cancer. Therefore, blocking of PGE2 generation has been identified as a promising approach for anticancer therapy. Sulforaphane (SFN), an isothiocyanate derived from glucosinolate, is used as the antioxidant and anticancer agents. METHODS: HT-29 cells were treated with various concentrations of SFN and compared to untreated cells for the expression of microsomal prostaglandin E synthase-1 (mPGES-1), cyclooxygenase 2 (COX-2), hypoxia-inducible factor-1 (HIF-1), C-X-C chemokine receptor type 4 (CXCR4), vascular endothelial growth factor (VEGF), and matrix metalloproteinase (MMP)-2 and MMP-9 at the mRNA level...
July 24, 2018: Cells, Tissues, Organs
Yu Feng, Xiang-Yang Tian, Peng Sun, Ze-Peng Cheng, Reng-Fei Shi
Mechanical stretch may cause myoblasts to either proliferate or undergo apoptosis. Identifying the molecular events that switch the fate of a stretched cell from proliferation to apoptosis is practically important in the field of regenerative medicine. A recent study on vascular smooth muscle cells illustrated that identification of these events may be achieved by addressing the stretch-induced opposite cellular outcomes simultaneously within a single investigation. To define conditions or a model in which both proliferation and apoptosis can be studied at the same time, we exposed in vitro cultured C2C12 myoblasts to a cyclic mechanical stretch regimen of 15% elongation at a stretching frequency of 1 Hz for 0, 2, 4, 6, or 8 h every day, consecutively, for 3 days...
June 27, 2018: Cells, Tissues, Organs
Hao Li, Jingkai Wang, Fangcai Li, Gang Chen, Qixin Chen
Nucleus pulposus-derived mesenchymal stem cells (NP-MSCs) are suitable cell candidates for intervertebral disc (IVD) regeneration. However, little work has been done to determine the proliferation and chondrogenic differentiation of NP-MSCs in the hyperosmotic microenvironment of IVD. This study aimed to investigate the influence of the hyperosmolarity of IVD on the proliferation and chondrogenic differ-entiation of NP-MSCs. NP-MSCs were cultured in media of 300, 400, 430, and 500 mOsm/L, mimicking the osmotic pressures of serious degenerative, moderately degenerative, and healthy IVD...
2018: Cells, Tissues, Organs
Saloni Balgi-Agarwal, Caitlyn Winter, Alexis Corral, Shamimunisa B Mustafa, Peter Hornsby, Alvaro Moreira
Mesenchymal stem cells (MSCs) have shown promise as therapeutic agents in treating morbidities associated with premature birth. MSCs derived from the human umbilical cord are easy to isolate and have low immunogenicity and a robust ability to secrete paracrine factors. To date, there are no studies evaluating preterm versus term umbilical cord tissue-derived MSCs. Therefore, our aim was twofold: (1) to compare stem cell properties in preterm versus term MSCs and (2) to examine the impact of oxygen tension on stem cell behavior...
2018: Cells, Tissues, Organs
Marko Jović, Ivan Nikolić, Vera Todorović, Aleksandar Petrović, Vladimir Petrović, Marijola Mojsilović, Tijana Denčić
In the absence of systematized data on the extracellular matrix components during prenatal liver development, the present study aimed to investigate the time of appearance and distribution of collagen types I, III, and IV and laminin. The study material included embryonic and fetal livers, aged 7-37 weeks, categorized into 3 trimesters. The material was stained using hematoxylin-eosin and immunohistochemistry methods for the identification of collagen I, III, and IV and laminin. Collagen I was detected near the end of the first trimester in the capsules and walls of interlobular veins...
2018: Cells, Tissues, Organs
Jeannette Attiger, Alois Boos, Karl Klisch
Binucleate trophoblast giant cells (TGCs) are one characteristic feature of the ruminant placenta. In cows, the frequency of TGCs remains constant for most of the duration of pregnancy. As TGCs are depleted by their fusion with uterine epithelial cells, they need to be constantly formed. It is still unclear whether they develop from stem cells within the trophectoderm or whether they can arise from any uninucleate trophoblast cell (UTC). Within the latter, generally accepted theory, a basally located uninucleate cell (BUC) without contact to the feto-maternal interface would represent a transient cell between a UTC and a TGC...
2018: Cells, Tissues, Organs
Reginaldo Franklin, Rodrigo Freitas Monte Bispo, Célio Fernando Sousa-Rodrigues, Lucas Alves Sarmento Pires, Albino Fonseca, Marcio Antonio Babinski
Nonalcoholic fatty liver disease (NAFLD) is a common ailment. It is usually found in association with diabetes or obesity. There are no approved drugs to treat this condition. The study of flavonoid consumption has increased over the decades due to their antioxidative properties, although the literature is scarce when it comes to their effects in liver tissue. The purpose of this study was to evaluate the role of leucoanthocyanidin in nonalcoholic hepatic steatosis. Thirty male albino rabbits were divided in 3 groups...
2018: Cells, Tissues, Organs
Lucio Díaz-Flores, Ricardo Gutiérrez, Miriam González-Gómez, Pino García, Francisco J Sáez, Lucio Díaz-Flores, José Luis Carrasco, Juan F Madrid
Vein segmentation is a vascular remodeling process mainly studied in experimental conditions and linked to hemodynamic factors, with clinical implications. The aim of this work is to assess the morphologic characteristics, associated findings, and mechanisms that participate in vein segmentation in humans. To this end, we examined 156 surgically obtained cases of hemorrhoidal disease. Segmentation occurred in 65 and was most prominent in 15, which were selected for serial sections, immunohistochemistry, and immunofluorescence procedures...
2018: Cells, Tissues, Organs
Rasha B Abd-Ellatief, Heba K Mohamed, Hassan I Kotb
To our knowledge, this is the first study which investigates the induction of neuroinflammation in rats using an acidic-saline model of fibromyalgia. It is well known that the hippocampus has a fundamental role in pain perception, and astrocytes play a crucial role in pain signaling. Our aim is to evaluate the ability of dexmedetomidine to attenuate the inflammatory responses induced in astrocytes. In a group of healthy rats, induction of chronic muscle pain by intramuscular injection of 100 µL of acidic saline on days 0 and 5 resulted in peripheral sensitization (measured using the von Frey test) and significant (p < 0...
2018: Cells, Tissues, Organs
Javad Hashemi, Parvin Pasalar, Masoud Soleimani, Ehsan Arefian, Reza Khorramirouz, Aram Akbarzadeh, Fariba Ghorbani, Seyed Ehsan Enderami, Abdol Mohammad Kajbafzadeh
INTRODUCTION: Diabetes is known as a worldwide disease with a great burden on society. Since therapeutic options cover a limited number of target points, new therapeutic strategies in the field of regenerative medicine are considered. Bioscaffolds along with islet cells would provide bioengineered tissue as a substitute for β-cells. The perfusion-decellularization technique is considered to create such scaffolds since they mimic the compositional, architectural, and biomechanical nature of a native organ...
2018: Cells, Tissues, Organs
Chiaki Endo, Tadasu Sato, Takehiro Yajima, Kaoru Igarashi, Hiroyuki Ichikawa
Immunohistochemistry for several neurochemical substances was performed on the human incisive papilla and other oral structures. Sodium channel alpha subunit 7 (SCN7A) protein-immunoreactive (IR) Schwann cells and protein gene product 9.5 (PGP 9.5)-IR nerve fibers made nerve plexuses beneath the epithelium of the palate, including the incisive papilla, tongue, and lip. SCN7A immunoreactivity could also be detected in lamellated and nonlamellated capsules of corpuscle endings. Lamellated SCN7A-IR corpuscle endings were mostly restricted to the mucous and cutaneous lips...
2018: Cells, Tissues, Organs
Dalia Ibrahim
The vomeronasal organ (VNO) is the peripheral receptor organ of the accessory olfactory system, which is responsible for both sexual and innate behaviors. The degree of neuronal differentiation and maturation of the vomeronasal receptor cells together with the verification of the presence of the solitary chemoreceptor cells (SCCs) in the VNO of Corriedale sheep were assessed using immunofluorescence. A protein gene product 9.5 (PGP 9.5), which is a neuronal marker recognized to be expressed in most neurons of vertebrate species, an olfactory marker protein (OMP) that is precise for mature olfactory receptor cells, and lastly phospholipase C-β2 (PLC-β2), a marker in the signal transduction pathway of SCCs, were all tested...
2018: Cells, Tissues, Organs
Bin Zhang, Bo Meng, Edward Viloria, Adrien Naveau, Bernhard Ganss, Andrew H Jheon
The aim of this study was to evaluate the role of epithelial signal transducer and activator of transcription 3 (STAT3) in mouse incisor amelogenesis. Since Stat3 is expressed in the epithelial component of developing and adult mouse teeth, we generated and analyzed Krt14Cre/+;Stat3fl/fl mutant mice in which Stat3 was inactivated in epithelia including ameloblast progenitors and ameloblasts, the cells responsible for enamel formation. Histological analysis showed little enamel matrix in mutant incisors compared to controls...
2018: Cells, Tissues, Organs
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"