P L Fracaro, C D Corcini, N L Souza Gatti, J S Filho, I B Acosta, J S Filho, F P Hartwig, B D Rosa Curcio, A S Varela Junior
BACKGROUND: Glycerol is a cryoprotectant widely used in the freezing of mammalian semen, but no study has demonstrated its optimum concentration and the appropriate exposure time for equine species. OBJECTIVE: To demonstrate that the exposure time (15, 30, 45, 60, 75 and 90 min) versus concentration (2, 3, 4 and 5%) of the cryoprotectant glycerol influences the freezing success of equine semen. MATERIALS AND METHODS: The ejaculate of 12 stallions were frozen in different glycerol concentrations following different exposure times...
2023: Cryo Letters