journal
https://read.qxmd.com/read/38032307/cryopreservation-of-seeds-of-neonotonia-wightii-wight-and-arn-a-strategy-for-conservation-dormancy-breaking-and-preservation-of-nutritional-status
#21
JOURNAL ARTICLE
Y Acosta, D Escalante, M E Martinez-Montero, D Fortes, B Z Zevallos-Bravo, E Hajari, D Fontes, J C Lorenzo
BACKGROUND: N. wightii (Leguminosae) is valued as a cover crop and as a potential source of protein in food insecure countries. However, plantlet establishment is limited by physical dormancy. Our previous work has shown that exposure of N. wightii seeds to cryogenic temperatures is able to overcome physical dormancy. OBJECTIVE: The current study is an extension of that work where the field performance and nutritional composition of plants regenerated from N. wightii seeds was investigated...
2023: Cryo Letters
https://read.qxmd.com/read/38032306/cryopreservation-of-lilium-candidum-germplasm-analysis-of-pre-and-post-freeze-treatments
#22
JOURNAL ARTICLE
H B Tokgoz, H Karakas, E Kaya, H Yildirim, A F Pirhan, F Altan
BACKGROUND: Lilium candidum L. is a perennial ornamental plant that has various medicinal properties and is used in the cosmetic industry. The species is facing threats from urbanization and climate change and requires urgent protection. The most secure and efficient technology for the long-term storage of plant genetic resources is cryopreservation, which involves preserving genetic material at extremely low temperatures. OBJECTIVE: Today, plant biodiversity is endangered because of the narrowing of its natural distribution areas and/or destruction for different purposes...
2023: Cryo Letters
https://read.qxmd.com/read/38032305/enhanced-heat-transfer-by-medical-gauze-for-cell-vitrification-with-french-straw
#23
JOURNAL ARTICLE
S Tao, B Liu
BACKGROUND: Film boiling occurs in the cooling process of samples with liquid nitrogen, which limits heat transfer and decreases the cooling rate. OBJECTIVE AND METHODS: The study developed a method to enhance convective heat transfer by wrapping the French straw (FS) with a layer of medical gauze upon cooling to eliminate film boiling. A numerical model was used to study the thermodynamic mechanism in the method. RESULTS: Numerical simulation based on heat transfer and crystallization equations indicated that wrapping the FS with medical gauze could suppress film boiling...
2023: Cryo Letters
https://read.qxmd.com/read/38032304/perspective-potential-and-reality-of-cryopreserving-somatic-cells-of-wild-felids-for-conservation
#24
JOURNAL ARTICLE
L L Vieira Rodrigues, A F Pereira
The loss of biodiversity caused by anthropogenic actions is also a reality for the members of the Felidae family. Except for the domestic cat, all felid species have some degree of threat of extinction in their natural habitat. For this reason, felids have been included in conservation-related studies. This scenario has aroused increasing interest in the formation of somatic cell banks, which when efficiently implemented can be used in preservation strategies for the species. Nevertheless, one of the important steps in the formation of these banks is the understanding of the technical principles and variations involved in cryopreservation techniques, especially because cryopreservation increases the possibilities for Assisted Reproduction Technologies (ARTs) by making the use of biological materials independent of time and space...
2023: Cryo Letters
https://read.qxmd.com/read/37883171/the-safety-of-human-embryos-following-long-term-cryopreservation-6-years-on-vitrification
#25
JOURNAL ARTICLE
H He, R Jiang, X Ren, L Jin, Y Jiang
BACKGROUND: Vitrification of embryos has become the basic means of assisted reproductive technology (ART) therapy in recent years. Concerns have also been raised about the safety of vitrification and the effect of cryopreservation time. Most of the previous studies were on the data within 6 years of cryopreservation. OBJECTIVE: In this study, we aimed to evaluate the impact of long-term cryopreservation (>6 years) on pregnancy and neonatal outcomes. MATERIALS AND METHODS: This research was a single-center, retrospective analysis, including 426 frozen-thawed embryo transfer (FET) cycles...
2023: Cryo Letters
https://read.qxmd.com/read/37883170/motility-viability-and-fertility-of-goldfish-carassius-auratus-pisces-cyprinidae-post-short-term-cryopreservation
#26
JOURNAL ARTICLE
N Nurlaili, K Eriani, I Salma, S Maulida, S R Rahayu, L S Handayani, F K Kocabas, M N Siti-Azizah, M Wilkes, Z A Muchlisin
BACKGRUND: Goldfish Carassius auratus is a popular ornamental fish extensively cultured worldwide. Sperm cryopreservation is a common fish breeding method that ensures sperm availability around the year. Studies on cryopreservation of goldfish sperm, especially on the suitability of cryoprotectant types and pre-freezing time, are scarcely available. OBJECTIVE: To determine the most suitable type of cryoprotectant and pre-freezing for the successful cryopreservation of goldfish sperm...
2023: Cryo Letters
https://read.qxmd.com/read/37883169/assessing-the-benefits-of-antioxidant-and-antibiotics-treatments-for-cryopreservation-of-the-model-alga-ectocarpus-siliculosus-and-the-endemic-alga-acinetospora-asiatica
#27
JOURNAL ARTICLE
J Avila-Peltroche, B Y Won, T O Cho
BACKGROUND: Cryopreservation in liquid nitrogen is a suitable technique for preserving seaweeds, a group of photosynthetic organisms with many applications. Although there are some standard protocols for seaweed cryopreservation, most rely on expensive controlled-rate coolers. Moreover, several factors, such as the use of antioxidants or antibiotics, remain unexplored. OBJECTIVE: To test the effect of 2-mercapthoethanol (antioxidant) and antibiotic mixtures on the cryopreservation of the model alga Ectocarpus siliculosus and the endemic brown seaweed Acinetospora asiatica using a low-tech passive rate cooler...
2023: Cryo Letters
https://read.qxmd.com/read/37883168/aqueous-extract-of-moringa-oleifera-lam-leaves-added-to-freezing-extenders-damages-goat-sperm-membranes
#28
JOURNAL ARTICLE
D C de Mello Seal, M Monteiro, L C Pereira Arruda, J H de Souza, R R de Vasconcelos Alves, L A Lira Soares, T H Napoleao, P M Guedes Paiva, P E Bezerra de Lima, R C Bressan Queiroz de Figueiredo, M M Pessoa Guerra
BACKGROUND: Semen cryopreservation is a biotechnology used frequently in animal production; however, there are some obstacles, such as those caused by high levels of reactive oxygen species (ROS). Moringa oleifera (MO) is known as a potent source of antioxidants and might be an important adjuvant. OBJECTIVE: The objective of this study was to determine the effect of different concentrations of MO extract supplementation on goat semen cryopreservation efficiency...
2023: Cryo Letters
https://read.qxmd.com/read/37883167/comparative-cryopreservation-of-indian-wild-orange-citrus-indica-tanaka-embryonic-axes
#29
JOURNAL ARTICLE
S K Malik, S Kaur, R Choudhary, R Chaudhury, H W Pritchard
BACKGROUND: Indian Wild Orange (Citrus indica Tanaka) is an endangered and endemic species from northeast India for which effective ex situ conservation strategies, including embryo cryopreservation, are urgently needed. MATERIALS AND METHODS: Desiccation tolerance and cryopreservation ability for embryonic axes of Citrus indica was determined using three techniques (air desiccation-freezing, PVS2 vitrification-freezing and encapsulation-dehydration-freezing). Success was assessed as survival and recovery in vitro...
2023: Cryo Letters
https://read.qxmd.com/read/37883166/effect-of-isolation-protocols-and-cryoprotectants-on-freezing-of-stallion-epididymal-spermatozoa
#30
JOURNAL ARTICLE
T R Talluri, D Jhamb, N Paul, J Singh, R K Dedar, S C Mehta, R A Legha, Y Pal
BACKGROUND: The recovery of spermatozoa from the cauda epididymis may be the only option to obtain genetic material from elite stallions that had undergone castration or sudden death due to colic or severe injury. OBJECTIVE: To evaluate two different protocols for retrieval of stallion epididymal spermatozoa and to evaluate different cryoprotectants on the freezability of the epididymal spermatozoa. MATERIALS AND METHODS: Six epididymides from three stallions were collected immediately after routine castration under general anesthesia...
2023: Cryo Letters
https://read.qxmd.com/read/37883165/the-role-of-cryopreservation-techniques-in-manufacturing-transport-and-storage-of-car-t-therapy-products
#31
JOURNAL ARTICLE
M Jandova, G N Stacey, M Lanska, I Gregor, P Rozsivalova, L Bekova, Z W Duchacova, D Belada, J Radocha, P Mericka, B Fuller
Several clinical trials have proved the efficacy and safety of T-cells chimeric antigen receptor (CAR-T cells) in treatment of malignant lymphoma and the first products were registered in the European Union in 2018. The shelf-life of CAR-T cell products in the liquid state is short, so cryopreservation offers a significant benefit for logistics in manufacturing and patient management. Direct shipment of the cryopreserved CAR-T cell therapy products to the clinical department is feasible, nevertheless, intermediate storage in the hospital cryostorage facility gives significant advantage in planning of their administration to patients...
2023: Cryo Letters
https://read.qxmd.com/read/37883162/vitrified-before-and-after-genome-editing-via-electroporation
#32
JOURNAL ARTICLE
T V Nguyen, L T Kim Do, Z Namula, Q Y Lin, N Torigoe, M Nagahara, M Hirata, F Tanihara, T Bio-Innovation Research Center Tokushima University Tokushima Japan Otoi
BACKGROUND: Cryopreservation of bovine zygotes allows for a flexible schedule of genome editing via electroporation. However, vitrification-induced cell membrane damage may not only affect embryonic development but also genome mutation. OBJECTIVE: To investigate the effects of vitrification of zygotes before and after electroporation treatments on the development and genome mutation of bovine presumptive zygotes. MATERIALS AND METHODS: In vitro-derived bovine zygotes were electroporated with the CRISPR/Cas9 system immediately (Vitrified-EP) or 2 h after incubation (Vitrified-2h-EP) following vitrification and warming, or electroporated before vitrification (EP-vitrified)...
2023: Cryo Letters
https://read.qxmd.com/read/37883161/effect-of-antioxidant-procyanidin-b2-pcb2-on-ovine-oocyte-developmental-potential-in-response-to-in-vitro-maturation-ivm-and-vitrification-stress
#33
JOURNAL ARTICLE
J Bai, J Li, L Wang, S Hao, Y Guo, Y Liu, Z Zhang, H Li, W Q Sun, G Shi, P Wan, X Fu
BACKGROUND: It was demonstrated that external stress, such as in vitro maturation (IVM) and vitrification process can induce significantly reduced development capacity in oocytes. Previous studies indicated that antioxidants play a pivotal part in the acquisition of adaptation in changed conditions. At present, the role of the natural potent antioxidant PCB2 in response to IVM and vitrification during ovine oocyte manipulation has not been explored. OBJECTIVE: To investigate whether PCB2 treatment could improve the developmental potential of ovine oocytes under IVM and vitrification stimuli...
2023: Cryo Letters
https://read.qxmd.com/read/37883160/interactions-among-sucrose-and-concentrations-of-serum-fetal-bovine-on-the-cryopreservation-of-somatic-cells-derived-from-red-rumped-agoutis
#34
JOURNAL ARTICLE
E A Praxedes, M B Silva, L R Medeiros de Oliveira, J V da Silva Viana, A F Pereira
BACKGROUND: The synergistic action among the different extracellular cryoprotectants could improve somatic cell quality after thawing and provide bases for the formation of biobanks for red-rumped agoutis. OBJECTIVE: This study evaluated the interactions among sucrose (SUC) and concentrations of serum fetal bovine (FBS) on the cryopreservation of somatic cells derived from red-rumped agoutis. MATERIALS AND METHODS: Cells were cryopreserved with 10% dimethyl sulfoxide and different concentrations of FBS (10%, 40%, and 90%) with or without 0...
2023: Cryo Letters
https://read.qxmd.com/read/37883159/camptothecin-solubility-in-vitro-drug-release-and-effect-on-human-red-blood-cells-and-sperm-cold-preservation
#35
JOURNAL ARTICLE
S Fatmi, L Taouzinet, M Skiba, M Iguer-Ouada
BACKGROUND: Camptothecin (CPT) is an anticancer drug, and is not employed in the clinic because of its high hydrophobicity and low active form stability. CPT may also have potential for use in cold preservation. OBJECTIVE: To overcome these drawbacks, CPT solubility variations in the presence of cyclodextrins (CDs) and polyethylene glycol (PEG) were evaluated by Higuchi solubility experiments. MATERIALS AND METHODS: CPT was encapsulated in different cyclodextrins and polyethylene glycol using a co-evaporation method...
2023: Cryo Letters
https://read.qxmd.com/read/37883158/human-follicular-fluid-and-mesenchymal-stem-cell-conditioned-medium-improves-in-vitro-development-of-vitrified-warmed-mouse-oocytes
#36
JOURNAL ARTICLE
S Geravandi, E Kalehoei, A Karami, F Nowrouzi, Z Kalhori, H Zhaleh, M Azadbakht
BACKGROUND: In vitro maturation (IVM) and oocyte cryopreservation are therapeutic options in assisted reproductive technology which is used to preserve fertility in patients with different causes of infertility. OBJECTIVE: To analyze in vitro development of vitrified-warmed oocytes in the presence of human follicular fluid (FF) and bone marrow mesenchymal stem cell-conditioned medium (BMSC-CM) as a rescue strategy in fertility preservation. MATERIALS AND METHODS: BMSC-CM and FF media were used as two natural media...
2023: Cryo Letters
https://read.qxmd.com/read/37883157/stabilization-of-labile-active-ingredients-in-an-oil-water-emulsion-cosmetics-by-freeze-drying
#37
JOURNAL ARTICLE
Z X Yi, M Yang, B L Liu
BACKGROUND: Due to the instability in oil/water emulsion, certain labile active ingredients were often not used in cosmetics. OBJECTIVE: The present study has tested the effect of freeze-drying to stabilize an oil/water cosmetic emulsion. MATERIALS AND METHODS: A preliminary freeze-drying process was established at the basis of calorimetric and freeze-drying microscope studies. The stability of labile molecules in the cosmetic emulsion was evaluated at 48 degree C after freeze-drying...
2023: Cryo Letters
https://read.qxmd.com/read/37883156/cryopreservation-of-organoids
#38
JOURNAL ARTICLE
O Rogulska, J Havelkova, Y Petrenko
Organoids represent indispensable opportunities for biomedicine, including drug discovery, cancer biology, regenerative and personalised medicine or tissue and organ transplantation. However, the lack of optimized preservation strategies limits the wide use of organoids in research or clinical fields. In this review, we present a short outline of the recent developments in organoid research and current cryopreservation strategies for organoid systems. While both vitrification and slow controlled freezing have been utilized for the cryopreservation of organoid structures or their precursor components, the controlled-rate slow freezing under protection of Me2SO remains the most common approach...
2023: Cryo Letters
https://read.qxmd.com/read/37883142/in-vitro-cold-acclimation-is-required-for-successful-cryopreservation-of-juglans-regia-l-shoot-tips
#39
JOURNAL ARTICLE
S V Kushnarenko, N K Rymkhanova, M M Aralbayeva, N V Romadanova
BACKGROUND: Persian walnut (Juglans regia L.) is one of the most economically important nut crops. In the Western Tien Shan in Kazakhstan, walnut forests are the northernmost in the natural range of this species. Shoot tip cryopreservation is an important strategy to ensure long-term clonal conservation of plant genetic resources. OBJECTIVE: To determine the effect of cold acclimation duration (0-6 weeks) with alternating temperatures (8 h at 22 degree C, light intensity 10 μmol m-2 s-1 /16 h at -1 degree C, in the dark) and of plant vitrification solution 2 (PVS2) exposure duration (30, 50, 80 or 100 min) on shoot tip regrowth after cryopreservation by vitrification...
2023: Cryo Letters
https://read.qxmd.com/read/37883141/freezing-of-equine-semen-is-influenced-by-exposure-time-and-concentration-of-the-cryoprotectant-glycerol
#40
JOURNAL ARTICLE
P L Fracaro, C D Corcini, N L Souza Gatti, J S Filho, I B Acosta, J S Filho, F P Hartwig, B D Rosa Curcio, A S Varela Junior
BACKGROUND: Glycerol is a cryoprotectant widely used in the freezing of mammalian semen, but no study has demonstrated its optimum concentration and the appropriate exposure time for equine species. OBJECTIVE: To demonstrate that the exposure time (15, 30, 45, 60, 75 and 90 min) versus concentration (2, 3, 4 and 5%) of the cryoprotectant glycerol influences the freezing success of equine semen. MATERIALS AND METHODS: The ejaculate of 12 stallions were frozen in different glycerol concentrations following different exposure times...
2023: Cryo Letters
journal
journal
34428
2
3
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.