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Cryo Letters

S Wada, B M Reed
BACKGROUND: Hop powdery mildew (HPM), Podosphaera macularis, an important disease organism for hops, is an obligate parasite, requiring constant culture on living plant tissue for strain maintenance. OBJECTIVE: This study determined the parameters required to successfully cryopreserve HPM spores for the first time and reduce the need for constant culture. MATERIALS AND METHODS: Spores of an Oregon HPM strain, OSU C-100 were desiccated over silica gel for 2-10 h to determine the spore moisture content (MC)...
May 2017: Cryo Letters
S Mehmood, H Ahmed, S A Hussain Shah, F H Wattoo, S Akhtar, S M Hassan Andrabi
BACKGROUND: There is a possibility to reduce the toxicity of glycerol and osmotic stress of DMSO by lowering their concentrations in freezing extenders. OBJECTIVE: To investigate the effect of glycerol and DMSO in tris-citric acid based extender on post- thaw quality of buffalo (Bubalus bubalis) bull spermatozoa. MATERIALS AND METHODS: Semen was collected from five adult buffalo bulls with artificial vagina. Five aliquots of semen per bull were separated for dilution with the treatment extenders...
May 2017: Cryo Letters
W Di, M X Jia, J Xu, B L Li, Y Liu
BACKGROUND: Reactive oxygen species (ROS)-induced oxidative damage is responsible for viability loss in plant tissues following cryopreservation. Antioxidants may improve viability by preventing or repairing the injury. OBJECTIVE: This work aimed at studying the effect of catalase (CAT) and pyruvate dehydrogenase (PDH), which are involved in ROS metabolism and are differentially expressed during pollen cryopreservation, for cryopreservation of Dendrobium nobile Lindl...
May 2017: Cryo Letters
X T Li, Y Wang, S S Lu, M Li, S K Men, Y C Bai, X L Tang, Q Chen
BACKGROUND: Phrynocephalus erythrurus living at Qinghai-Tibet Plateau, is believed to be the highest lizard in the world, but we know little about how these lizards cope with very low temperatures in winter. OBJECTIVE: The aim of this study was to find the difference of the lizards before and after cold acclimatization. MATERIALS AND METHODS: In this study the limit of supercooling and inoculative freezing, the concentration of four organic osmolytes, and the activity of lactate dehydrogenase in the plasma were measured in samples shortly after capture and in other samples after 7~8 weeks of acclimatization at 2~4 degree C...
May 2017: Cryo Letters
J O Daramola, E O Adekunle
BACKGROUND: Sperm washing in some species helps remove the detrimental effect of enzymes secreted from the bulbourethral gland, but such an effect has not been evaluated for cryopreserved sperm obtained from West African Dwarf (WAD) goat bucks. OBJECTIVE: This study evaluated the effects of washing protocols on the viability parameters of spermatozoa obtained from WAD goat bucks prior to cryopreservation. MATERIALS AND METHODS: Semen samples collected from WAD goat bucks with the aid of artificial vagina were subjected to washing protocols by centrifuging once, twice and thrice while the control group was not washed...
May 2017: Cryo Letters
I A Montalban, P Moncalean
BACKGROUND: Pinus radiata is an economically important conifer, and somatic embryogenesis is being currently used for its propagation. But the embryogenic competence of cultures decreases with culture age. To cope with this, cryopreservation protocols have been developed lately for different Pinus species. Although cryopreservation reduces the costs associated with embryogenic cultures maintenance, the initial investment and the maintenance of cryotanks are expensive when dealing with somatic embryogenesis basic research issues...
May 2017: Cryo Letters
B J Kang, Y Wang, L Zhang, S W Li
BACKGROUND: Basic fibroblast growth factor is a potent angiogenic factor. OBJECTIVE: To study the concentration and in vitro culture time of bFGF that maximize the angiogenesis for transplanted human ovarian tissues. MATERIALS AND METHODS: Vitrified and rewarmed human ovarian tissues were cultured in vitro with bFGF (0, 25, 50, 100, 150 and 200 ng/ml) for different periods (1 h, 2 d, 5 d and 7 d) before transplantation. The effect of bFGF on follicle survival was studied by evaluating the pregraft group, control group (no bFGF) and bFGF-treated group...
May 2017: Cryo Letters
G DA Gastal, E F Silva, B Mion, A S Varela Junior, C E Rosa, C D Corcini, R G Mondadori, A D Vieira, I Bianchi, T Lucia
BACKGROUND: Xanthan gum is used as thickener in media to preserve food products, having cryoprotectant and antioxidant properties that may be relevant for sperm cryopreservation. OBJECTIVE: To evaluate the effects of adding xanthan gum to freezing extenders on post-thawing quality and oxidant activity of ram sperm. METHODS: Ejaculates from seven rams extended TRIS-egg yolk-glycerol were split in three treatments including xanthan gum (0.15%; 0...
May 2017: Cryo Letters
M H Le
BACKGROUND: The cryopreservation protocols have been applied for sperm prevervation of many fish species, but have not been developed for Waigieu seaperch Psammoperca waigiensis, an important aquaculture species in Vietnam. OBJECTIVE: The objectives of this study were to find the best cryoprotectant, extender, freezing method and dilution ratio for sperm cryopreservation of Waigieu seaperch. MATERIALS AND METHODS: An effective protocol was established by comparing different cryodiluents created by mixing various cryoprotectants and extenders...
May 2017: Cryo Letters
V K Jasmi, F Samadi, H Eimani, S Hasani, R Fathi, A Shahverdi
BACKGROUND: Ovarian cryopreservation has emerged as an important method of fertility preservation. Magnetic field enhanced cryopreservation has been considered in recent times as a promising type of ovarian cryopreservation but the effectiveness of the process is still not clear. OBJECTIVE: The aim of the present study was to investigate the effect of applying 1-mT SMF (static magnetic field) on the vitrification of ovarian tissue and the follow-up investigation of the morphology and functions of vitrified- warmed ovarian tissue after transplantation...
May 2017: Cryo Letters
W Korchunjit, K Kaeoket, Y Kitiyanant, J Taylor, T Wongtawan
BACKGROUND: It is believed that plasma membrane X- and Y-chromosome bearing sperm are different; therefore the freezing and thawing process may affect X- and Y-sperm differently. OBJECTIVE: The objective of this study was to investigate the effect of cryomedia and thawing extenders on the survival of X and Y-sperm. MATERIALS AND METHODS: Three different cryomedia and thawing extenders were compared. Viable motile sperm were separated using a swim-up technique...
May 2017: Cryo Letters
B Funnekotter, E Bunn, R L Mancera
BACKGROUND: The continued development of new cryopreservation protocols has improved post-cryogenic success rates for a wide variety of plant species. Methods like the cryo-plate have proven beneficial in simplifying the cryopreservation procedure. OBJECTIVE: This study assessed the practicality of a stainless steel mesh strip (cryo-mesh) for cryopreserving shoot tips from Anigozanthos viridis. MATERIALS AND METHODS: Shoot tips of A. viridis (Kangaroo Paw) were precultured on 0...
March 2017: Cryo Letters
L Ali, S M Hassan Andrabi, H Ahmed, A A Hussain Shah
BACKGROUND: Catalase enzyme is usually distributed in mammalian seminal plasma, where it decomposes hydrogen peroxide into water and oxygen and enhances sperm survivability. OBJECTIVE: To evaluate the effect of catalase (0, 100, 200 or 300 IU/ml) added in tris-citric acid (TCA) based extender on motion characteristics, viability and DNA integrity of bubaline spermatozoa at post dilution (PD) and post thawing (PT) stages of cryopreservation. MATERIALS AND METHODS: Collection of semen was done in four Nili-Ravi bulls with an artificial vagina (42 degree C)...
March 2017: Cryo Letters
T Morewood, N Getreu, B Fuller, J Morris, P Hardiman
BACKGROUND: Ovarian tissue cryopreservation has the potential to improve fertility preservation for a growing number of patients undergoing sterilising therapy, particularly where oocyte or embryo cryopreservation is not suitable. However, its success is limited by significant follicular apoptosis upon thawing, and there is wide variation in thawing protocols used with little evidence of efficacy. OBJECTIVE: To determine the best warming rates to maintain tissue viability...
March 2017: Cryo Letters
S M Chen, L Ren, D Zhang, Y F Zhang, X H Shen
BACKGROUND: Adding exogenous compounds is an effective way to improve cell survival after cryopreservation. Carbon nanomaterials (CNMs) are novel exogenous substances with small particle size and good biocompatibility. OBJECTIVE: In this work, four types of CNMs were used for the cryopreservation of Agapanthus praecox callus and their possible effects and mechanism of action were analyzed. MATERIALS AND METHODS: The thermal properties of the vitrification solutions tested were detected by differential scanning calorimetry...
March 2017: Cryo Letters
M V Santos, M Sansinena, N Zaritzky, J Chirife
BACKGROUND: Dry ice-ethanol bath (-78 degree C) have been widely used in low temperature biological research to attain rapid cooling of samples below freezing temperature. The prediction of cooling rates of biological samples immersed in dry ice-ethanol bath is of practical interest in cryopreservation. The cooling rate can be obtained using mathematical models representing the heat conduction equation in transient state. Additionally, at the solid cryogenic-fluid interface, the knowledge of the surface heat transfer coefficient (h) is necessary for the convective boundary condition in order to correctly establish the mathematical problem...
March 2017: Cryo Letters
S Geravandi, M Azadbakht
BACKGROUND: Cryopreservation of ovary is a relevant option for preserving fertility of cancer patients undergoing chemotherapy. OBJECTIVE: To evaluate the effect of zinc in the vitrification medium on histology and follicle growth. MATERIALS AND METHODS: Mouse ovaries were vitrified in vitrification medium supplemented with 0, 100, 150 or 200 ╬╝g/dl zinc, identified as V0, V1, V2 and V3 groups, respectively. Histological evaluation of ovaries was carried out...
March 2017: Cryo Letters
H E Tomalty, E F Hamilton, A Hamilton, O Kukal, T Allen, V K Walker
BACKGROUND: Contemporary kidney preservation methods involve storing at 4 degree C up to 24 h prior to transplantation. By decreasing the storage temperature to below 0 degree C, we hypothesized that the safe storage time could be significantly lengthened. OBJECTIVE: The efficacy of a proprietary CryoStasis (CrS) storage solution for the subzero preservation of kidneys was tested, with or without addition of a hyperactive insect antifreeze protein (TmAFP). MATERIALS AND METHODS: Rat kidneys were stored in either University of Wisconsin (UW) solution (4 degree C, 24 h), CrS (-2 degree C, 48 h), or CrS with 61...
March 2017: Cryo Letters
R Parameswari, K A Rao, P Manigandan, A S Vickram, A Archana, T B Sridharan
BACKGROUND: Antioxidants protect spermatozoa against lipid peroxidation during freezing. OBJECTIVE: The study is designed to elucidate the suitable extender to preserve infertile semen of smokers against ROS damage using natural Tea polyphenol (T. arjuna bark extract). MATERIALS AND METHODS: Forty-two infertile subjects with smoking habit and 28 fertile subjects without smoking habit were considered for the study. Four semen extenders including our naturally derived antioxidant component were prepared and used to preserve semen sample from the study subjects for a period of one month...
March 2017: Cryo Letters
A S Varela, T F Cardoso, E Fernandes E Silva, K L Goularte, M H Okamoto, L A Sampaio, R D Jardim, C D Corcini
BACKGROUND: Paralichthys orbignyanus is the species of the greatest potential for marine and estuarine fish farming in southern Brazil. Consequently, embryo cryopreservation becomes an important tool for increasing their production. OBJECTIVE: To evaluate the effects of cooling protocols on the viability of embryos of P. orbignyanus at two stages of development (neurula and early differentiation of the tail). MATERIALS AND METHODS: Control embryos were maintained at 23 degree C and treated embryos were cooled to 15 degree C, 10 degree C and 5 degree C at rapid, moderate and slow cooling rates...
March 2017: Cryo Letters
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