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Cryo Letters

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https://www.readbyqxmd.com/read/28534059/cryo-mesh-a-simple-alternative-cryopreservation-protocol
#1
B Funnekotter, E Bunn, R L Mancera
BACKGROUND: The continued development of new cryopreservation protocols has improved post-cryogenic success rates for a wide variety of plant species. Methods like the cryo-plate have proven beneficial in simplifying the cryopreservation procedure. OBJECTIVE: This study assessed the practicality of a stainless steel mesh strip (cryo-mesh) for cryopreserving shoot tips from Anigozanthos viridis. MATERIALS AND METHODS: Shoot tips of A. viridis (Kangaroo Paw) were precultured on 0...
March 2017: Cryo Letters
https://www.readbyqxmd.com/read/28534058/freezability-of-water-buffalo-spermatozoa-is-improved-with-the-addition-of-catalase-in-cryodiluent
#2
L Ali, S M Hassan Andrabi, H Ahmed, A A Hussain Shah
BACKGROUND: Catalase enzyme is usually distributed in mammalian seminal plasma, where it decomposes hydrogen peroxide into water and oxygen and enhances sperm survivability. OBJECTIVE: To evaluate the effect of catalase (0, 100, 200 or 300 IU/ml) added in tris-citric acid (TCA) based extender on motion characteristics, viability and DNA integrity of bubaline spermatozoa at post dilution (PD) and post thawing (PT) stages of cryopreservation. MATERIALS AND METHODS: Collection of semen was done in four Nili-Ravi bulls with an artificial vagina (42 degree C)...
March 2017: Cryo Letters
https://www.readbyqxmd.com/read/28534057/the-effect-of-thawing-protocols-on-follicle-conservation-in-human-ovarian-tissue-cryopreservation
#3
T Morewood, N Getreu, B Fuller, J Morris, P Hardiman
BACKGROUND: Ovarian tissue cryopreservation has the potential to improve fertility preservation for a growing number of patients undergoing sterilising therapy, particularly where oocyte or embryo cryopreservation is not suitable. However, its success is limited by significant follicular apoptosis upon thawing, and there is wide variation in thawing protocols used with little evidence of efficacy. OBJECTIVE: To determine the best warming rates to maintain tissue viability...
March 2017: Cryo Letters
https://www.readbyqxmd.com/read/28534056/carbon-nanomaterials-enhance-survival-of-agapanthus-praecox-callus-after-cryopreservation-by-vitrification
#4
S M Chen, L Ren, D Zhang, Y F Zhang, X H Shen
BACKGROUND: Adding exogenous compounds is an effective way to improve cell survival after cryopreservation. Carbon nanomaterials (CNMs) are novel exogenous substances with small particle size and good biocompatibility. OBJECTIVE: In this work, four types of CNMs were used for the cryopreservation of Agapanthus praecox callus and their possible effects and mechanism of action were analyzed. MATERIALS AND METHODS: The thermal properties of the vitrification solutions tested were detected by differential scanning calorimetry...
March 2017: Cryo Letters
https://www.readbyqxmd.com/read/28534055/experimental-determination-of-surface-heat-transfer-coefficient-in-a-dry-ice-ethanol-cooling-bath-using-a-numerical-approach
#5
M V Santos, M Sansinena, N Zaritzky, J Chirife
BACKGROUND: Dry ice-ethanol bath (-78 degree C) have been widely used in low temperature biological research to attain rapid cooling of samples below freezing temperature. The prediction of cooling rates of biological samples immersed in dry ice-ethanol bath is of practical interest in cryopreservation. The cooling rate can be obtained using mathematical models representing the heat conduction equation in transient state. Additionally, at the solid cryogenic-fluid interface, the knowledge of the surface heat transfer coefficient (h) is necessary for the convective boundary condition in order to correctly establish the mathematical problem...
March 2017: Cryo Letters
https://www.readbyqxmd.com/read/28534054/the-presence-of-zinc-in-the-mouse-ovary-vitrification-medium-histological-evaluation-and-follicle-growth
#6
S Geravandi, M Azadbakht
BACKGROUND: Cryopreservation of ovary is a relevant option for preserving fertility of cancer patients undergoing chemotherapy. OBJECTIVE: To evaluate the effect of zinc in the vitrification medium on histology and follicle growth. MATERIALS AND METHODS: Mouse ovaries were vitrified in vitrification medium supplemented with 0, 100, 150 or 200 μg/dl zinc, identified as V0, V1, V2 and V3 groups, respectively. Histological evaluation of ovaries was carried out...
March 2017: Cryo Letters
https://www.readbyqxmd.com/read/28534053/kidney-preservation-at-subzero-temperatures-using-a-novel-storage-solution-and-insect-ice-binding-proteins
#7
H E Tomalty, E F Hamilton, A Hamilton, O Kukal, T Allen, V K Walker
BACKGROUND: Contemporary kidney preservation methods involve storing at 4 degree C up to 24 h prior to transplantation. By decreasing the storage temperature to below 0 degree C, we hypothesized that the safe storage time could be significantly lengthened. OBJECTIVE: The efficacy of a proprietary CryoStasis (CrS) storage solution for the subzero preservation of kidneys was tested, with or without addition of a hyperactive insect antifreeze protein (TmAFP). MATERIALS AND METHODS: Rat kidneys were stored in either University of Wisconsin (UW) solution (4 degree C, 24 h), CrS (-2 degree C, 48 h), or CrS with 61...
March 2017: Cryo Letters
https://www.readbyqxmd.com/read/28534052/tea-polyphenol-t-arjuna-bark-as-sperm-antioxidant-extender-in-infertile-smokers
#8
R Parameswari, K A Rao, P Manigandan, A S Vickram, A Archana, T B Sridharan
BACKGROUND: Antioxidants protect spermatozoa against lipid peroxidation during freezing. OBJECTIVE: The study is designed to elucidate the suitable extender to preserve infertile semen of smokers against ROS damage using natural Tea polyphenol (T. arjuna bark extract). MATERIALS AND METHODS: Forty-two infertile subjects with smoking habit and 28 fertile subjects without smoking habit were considered for the study. Four semen extenders including our naturally derived antioxidant component were prepared and used to preserve semen sample from the study subjects for a period of one month...
March 2017: Cryo Letters
https://www.readbyqxmd.com/read/28534051/cooling-strategies-for-brazilian-flounder-paralichthys-orbignyanus-embryos
#9
A S Varela, T F Cardoso, E Fernandes E Silva, K L Goularte, M H Okamoto, L A Sampaio, R D Jardim, C D Corcini
BACKGROUND: Paralichthys orbignyanus is the species of the greatest potential for marine and estuarine fish farming in southern Brazil. Consequently, embryo cryopreservation becomes an important tool for increasing their production. OBJECTIVE: To evaluate the effects of cooling protocols on the viability of embryos of P. orbignyanus at two stages of development (neurula and early differentiation of the tail). MATERIALS AND METHODS: Control embryos were maintained at 23 degree C and treated embryos were cooled to 15 degree C, 10 degree C and 5 degree C at rapid, moderate and slow cooling rates...
March 2017: Cryo Letters
https://www.readbyqxmd.com/read/28534050/cryopreservation-of-lilium-martagon-l-meristems-by-droplet-vitrification-and-evaluation-of-their-physiological-stability
#10
M Urbaniec-Kiepura, A Bach
BACKGROUND: The aim of the present study was to compare different strategies for cryopreservation of martagon lily meristems and to evaluate the physiological status of the regenerants. MATERIALS AND METHODS: The bulblets were stored at 5 degree C or 20 degree C and pretreated with 3% or 6% sucrose prior to droplet-vitrification. The meristems were then assessed for their survival and regeneration. Their photochemical activity was investigated using a Photosynthesis Yield Analyzer MINI PAM 2000 Portable Chlorophyll Fluorometer and their photosynthesis oxygen production was evaluated with a Plant Vital 5030 device...
March 2017: Cryo Letters
https://www.readbyqxmd.com/read/28376143/comparison-of-mitochondrial-function-in-boar-and-bull-spermatozoa-throughout-cryopreservation-based-on-jc-1-staining
#11
COMPARATIVE STUDY
C H Hu, X J Zhuang, Y M Wei, M Zhang, S S Lu, Y Q Lu, X G Yang, K H Lu
BACKGROUND: Poor reproductivity hampers the commercialization of cryopreserved boar semen. OBJECTIVE: This study was to determine the differences in the sperm mitochondrial function between boar and bull semen at different cryopreservation stages. MATERIALS AND METHODS: Boar and bull fresh, equilibrated, and frozen-thawed spermatozoa were evaluated for mitochondrial function using JC-1 under a fluorescent microscope. RESULTS: Bull and boar percentage of spermatozoa staining green (PSSG) showed no difference between fresh and equilibrated semen (P> 0...
January 2017: Cryo Letters
https://www.readbyqxmd.com/read/28376142/characterization-and-expression-analysis-of-attacins-antimicrobial-peptide-encoding-genes-from-the-desert-beetle-microdera-punctipennis-in-response-to-low-temperatures
#12
J Q Li, X Y Lu, J Ma
BACKGROUND: Antimicrobial peptides (AMPs) constitute the insect innate immune defense system. AMP production is usually induced by microbes. However, mounting evidence links insect immune reaction to cold hardiness. OBJECTIVE: We aimed at characterizing two attacins, MpAtt1 and MpAtt2, from the desert beetle Microdera punctipennis in Coleoptera and investigating the expression profiles of the two genes in response to cold stress. RESULTS: Full lengths MpAtt1 and MpAtt2 cDNAs were obtained from low temperature transcriptomic data...
January 2017: Cryo Letters
https://www.readbyqxmd.com/read/28376141/viability-and-infectivity-of-tuber-borchii-after-cryopreservation
#13
F Piattoni, P Leonardi, B Siham, M Iotti, A Zambonelli
BACKGROUND: Truffles (Tuber spp.) are the most precious ectomycorrhizal edible mushrooms whose biodiversity is seriously endangered. OBJECTIVE: To develop a protocol for cryopreservation of Tuber spp. mycelia using T. borchii as a model species, verifying whether conservation in liquid nitrogen may affect viability, growth rate, hyphal morphology and infectivity. MATERIALS AND METHODS: Cryopreservation was performed using sorbitol, sucrose and DMSO as cryoprotectants...
January 2017: Cryo Letters
https://www.readbyqxmd.com/read/28376140/effect-of-monosaccharides-in-glycerol-free-tris-extender-on-reactive-oxygen-species-and-apoptosis-in-dog-sperm-cryopreservation
#14
M A Rahman, S H Park, I J Yu
BACKGROUND OBJECTIVE: The present study investigated the effect of monosaccharides in a glycerol-free tris (GFT) extender on reactive oxygen species (ROS) levels and apoptosis in cryopreserved dog spermatozoa. MATERIALS AND METHODS: The sperm pellets were resuspended (5 × 10(7) mL(-1)) with GFT containing 172 mM glucose (G), 86 mM glucose + 86 mM fructose (GF), 86 mM glucose + 86 mM galactose (Gg), 172 mM fructose (F), 172 mM galactose (g) or 86 mM fructose + 86 mM galactose (Fg)...
January 2017: Cryo Letters
https://www.readbyqxmd.com/read/28376139/cryopreservation-of-nili-ravi-buffalo-bull-sperm-in-cryodiluent-supplemented-with-lolium-perenne-protein-preparations
#15
S Qadeer, M A Khan, M S Ansari, B A Rakha, R Ejaz, A U Husna, A Azam, N Ullah, V K Walker, S Akhter
BACKGROUND: Semen from the Nili-Ravi buffalo bull, Bubalus bubalis, shows poor survival after freeze storage compared to bovine (Bos taurus and Bos indicus) semen. Freeze-susceptibility distinctions in these two genera have been attributed to differences in sperm membranes. MATERIALS AND METHODS: We measured the impact of protein preparations derived from a frost-resistant perennial grass, Lolium perenne, with ice recrystallization inhibition activity on the low temperature storage of B...
January 2017: Cryo Letters
https://www.readbyqxmd.com/read/28376138/numerical-and-experimental-investigation-on-water-me2so-mixing-in-microfluidic-device-for-oocyte-cryopreservation
#16
Y Yang, X L Zhou, N F Zhou, W Q Shao, L R Tao
BACKGROUND: Oocyte vitrification is a practical tool in assisted reproductive technology and fertility preservation. However, vitrification requires high concentration of cryoprotectants (CPAs), which may cause osmotic injury and is toxic to oocytes during the addition and removal process of CPAs. OBJECTIVE: To minimize osmotic and toxic injuries, a microfluidic device was designed to provide continuous concentration changes of CPAs to oocytes. METHODS: Numerical simulations were first used to investigate the effects of geometric parameters of microchannel on water- Me2SO mixing performance...
January 2017: Cryo Letters
https://www.readbyqxmd.com/read/28376137/effects-of-tempol-and-quercetin-on-human-sperm-function-after-cryopreservation
#17
L Azadi, M Tavalaee, M R Deemeh, M Arbabian, M H Nasr-Esfahani
  BACKGROUND: Quercetin is a flavonoid with high reactive oxygen species (ROS) scavenging and ion chelating activity. It also enhances the activity of antioxidant enzymes and reduces enzymatic activity such as NADPH oxidase and NADH-dependent oxido-reductase. Tempol, as a superoxide dismutase mimetic agent, converts superoxide to less toxic hydrogen peroxide (H2O2), but cannot reduce highly toxic hydroxyl radicals in Fenton or Haber-Weiss reactions mediated with free iron or cupper. OBJECTIVE: The study was to compare the effect of Quercetin and Tempol in an optimized commercial cryo-protective media on ROS induced cryoinjury for the first time...
January 2017: Cryo Letters
https://www.readbyqxmd.com/read/28376136/cold-resistance-of-an-ant-camponotus-herculeanus-hymenoptera-formicidae-in-various-climates-in-north-east-asia
#18
D I Berman, Z A Zhigulskava, A N Leirikh
  BACKGROUND: The relationship between cold resistance of widespread invertebrate species and overwintering temperatures has not been studied; however, this knowledge could have high prognostic value. OBJECTIVE: Using an ant species Camponotus herculeanus to investigate whether there is a relationship between cold resistance and overwintering temperatures in two climatically distinct regions (coastal and continental). MATERIALS AND METHODS: We determined seasonal changes in SCP and LТ for overwintering ant stages, as well as winter temperatures in the nests...
January 2017: Cryo Letters
https://www.readbyqxmd.com/read/28376135/a-simple-and-efficient-protocol-for-the-cryopreservation-of-zygotic-embryos-of-macaw-palm-acrocomia-aculeata-jacq-lodd-ex-mart-a-tropical-species-with-a-capacity-for-biofuel-production
#19
Z G Luis, E Scherwinski-Pereira
BACKGROUND: The development of cryopreservation protocols for zygotic embryos has the advantage of direct embryo regeneration into plantlets, an important factor when one´s purpose is to preserve genetic plant resources. OBJECTIVE: Current study developed an efficient and simplified cryopreservation protocol for zygotic embryos of the macaw palm, a type of palm tree with great capacity for biodiesel production. MATERIALS AND METHODS: Embryos were obtained from seeds and rehydrated for 15 h in a 3% sucrose medium...
January 2017: Cryo Letters
https://www.readbyqxmd.com/read/28376134/the-beneficial-effect-of-carboxylated-poly-l-lysine-on-cryosurvival-of-vitrified-early-stage-embryos
#20
Y Tsuji, T Iwasaki, H Ogata, Y Matsumoto, S Kokeguchi, K Matsumura, S H Hyon, M Shiotani
BACKGROUND: In the vitrification of embryos, dimethyl sulfoxide (DMSO) is one of the most effective cryoprotectant agents (CPAs), but cytotoxic effects of DMSO on embryos are well known. Carboxylated poly-L-lysine (CPLL) has been identified as an effective cryoprotectant of cultured cell lines and mammalian oocytes. OBJECTIVE: To evaluate the efficacy and safety of CPLL as a CPA for developmental stage embryos. MATERIALS AND METHODS: Mouse 8-cell embryos and blastocysts were vitrified with ethylene glycol (EG), DMSO/EG, or CPLL/EG and the developmental potency assessed in vitro...
January 2017: Cryo Letters
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