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Cryo Letters

V H Do, S Walton, S Catt, A W Taylor-Robinson
BACKGROUND: Modifications to in vitro maturation (IVM) media are made to improve rates of blastocyst formation and quality of mammalian embryos. Embryo quality is an important factor in the viability of embryos following cryopreservation. Salubrinal is a specific inhibitor of endoplasmic reticulum stress-induced apoptosis in eukaryotic cells. Here, it was added to IVM medium in an attempt to increase blastocyst formation and to enhance embryo quality in cattle. OBJECTIVE: To assess the effect on blastocyst formation and cryotolerance of the supplementation of salubrinal to bovine IVM medium...
May 2018: Cryo Letters
K Zarzycka, I Pieszynski, B Szrajber, J Kujawa, M Zarzycki, P Kolasa
BACKGROUND: Cryotherapy is one of the methods used in physical medicine to achieve analgesic, anti-inflammatory, antiedematous effects and to reduce muscle tension. OBJECTIVE: The aim of the study was to assess the impact of systemic cryotherapy combined with kinesitherapy on systolic and diastolic blood pressure. MATERIALS AND METHODS: A group of 215 individuals of both sexes was selected and submitted to a two-week cycle of the systemic cryotherapy treatment combined with kinesiotherapy...
May 2018: Cryo Letters
Y Xian, B Li, P Pan, Y Wang, X Pei, Y Yang
BACKGROUND: Ovarian cryopreservation by vitrification and transplantation are useful methods to recover female fertility after radiotherapy and chemotherapy. As type II programmed cell death, autophagy plays important roles in ovarian follicle development, ovarian follicle atresia and anti-stress injury. OBJECTIVE: The potential role of autophagy in ovarian vitrification was investigated. MATERIALS AND METHODS: Mouse ovaries were cryopreserved by vitrification, and autophagy was treated, after which the ovarian histology was checked, and ovarian follicles were counted...
May 2018: Cryo Letters
J Jeong, S J Sa, H Chung, S Baek, I Choi
BACKGROUND: Cryopreserved semen is useful for animal breeding via artificial insemination (AI); however, the use of frozen-thawed boar sperm is limited due to cryodamage. OBJECTIVE: The goal of this study was to improve post-thaw motility of boar semen by supplementing the thawing medium with a phosphodiesterase inhibitor, Zardaverine. MATERIALS AND METHODS: Thawed boar semen samples were treated with different concentrations of Zardaverine (0, 20, 50, 75, 100 µM) and the motility was evaluated using a computer-assisted sperm analyser...
May 2018: Cryo Letters
J Rymaszewska, K M Urbanska, D Szczesniak, B Stanczykiewicz, E Trypka, A Zablocka
BACKGROUND: Mild Cognitive Impairments (MCI) and dementia are still incurable. The Whole-Body Cryotherapy (WBC) - short, cyclic exposure to extremely low temperatures - has proven anti-inflammatory and anti-oxidative effects. It can also induce hormonal, lipid and neural changes. OBJECTIVE: To evaluate the WBC effect on cognitive functioning and blood parameters of MCI patients. MATERIALS AND METHODS: Participants with MCI undertook 10 WBC sessions...
May 2018: Cryo Letters
A Kodym, A Senula, E M Temsch, R Hood-Nowotny, F Schumacher, O N Sekurova, S B Zotchev, M Kiehn
BACKGROUND: Artemisia laciniata, mainly distributed in Siberia and Central Asia, is classified as critically endangered in Europe. OBJECTIVES: This study developed a protocol for its micropropagation and cryopreservation. MATERIALS AND METHODS: In vitro cultures from fresh seed and in vivo shoots were initiated. Micropropagation and cryopreservation protocols were developed. Bacteria detected after cryopreservation were investigated using 16S rRNA analysis. Genome size measurements of regenerated plants after cryopreservation using flow cytometry and carbon isotope measurements to evaluate stress status were also carried out...
May 2018: Cryo Letters
B Tian, Y Feng, L Ren, T Wang, S Zong
BACKGROUND: Larvae of the Cossidae family moth Eogystia hippophaecolus bore into and overwinter in the roots of sea buckthorn, which damages this plant in China. OBJECTIVE: The primary aims of the current study were to investigate the effects of fatty acids on cold hardness in overwintering larvae. MATERIALS AND METHODS: The supercooling point (SCP), low temperature mortality and fatty acid composition of different overwintering larvae were assessed. RESULTS: E...
May 2018: Cryo Letters
D Yeni, M E Inanc, F Avdatek, P B Tuncer, B Cil, R Turkmen, U Tasdemir
BACKGROUND: Cryopreservation has a side effect on the motility, chromatin integrity and viability of sperm cells. OBJECTIVE: The present study investigated the effects of supplementation with rosmarinic acid (RA) Tris extender on sperm quality parameters, plasma and acrosome membrane damage, antioxidant enzyme activity and chromatin integrity following the freeze thawing process on bull spermatozoa. MATERIALS AND METHODS: Ejaculates were split into five aliquots and diluted to a final concentration of 15x106 spermatozoa per ml with the Tris extender containing RA (25, 50, 100 and 200 microgram per ml) and (control) and then frozen at a controlled rate...
March 2018: Cryo Letters
E J Hoekman, K E Tucker, C Am Jansen, C G Hilders
BACKGROUND: Oocyte vitrification is important for fertility preservation. There are debates, however, surrounding the components of the procedure itself. OBJECTIVE: before starting a vitrification program, we decided to (1) determine if blastocysts derived from previously frozen mouse embryos would be suitable to practice vitrification and (2) to analyze the factors that contributed to improving our "learning curve". MATERIALS AND METHODS: 58 expanded blastocysts cultured from commercially available frozen 1-cell mouse embryos (B6D2F1 x B6C3F1) were used...
March 2018: Cryo Letters
C Kumari, A Kumar, S K Sarangi, A Thirugnanam
BACKGROUND: Cryogen spray cooling is an effective method to treat nodular basal cell carcinoma. OBJECTIVE: To study the effect of spraying distance (z = 27 mm, 18 mm and 9 mm) on a nodular gel phantom and to validate the numerical model. MATERIALS AND METHODS: Liquid nitrogen is sprayed on agarose gel phantom using 0.8 mm nozzle diameter. A two-dimensional Pennes equation with phase change is solved on the axisymmetric non-orthogonal grid...
March 2018: Cryo Letters
T V Nguyen, F Tanihara, M Hirata, T Hirano, K Nishio, L T Kim Do, T V Nguyen, M Nii, T Otoi
BACKGROUND: Short-term storage is valuable method to reuse manipulated embryos. OBJECTIVE: The present study evaluated the effects of antifreeze protein (AFP) supplementation on the quality and development of in vitro-produced porcine morulae after short-term storage (24 h). MATERIALS AND METHODS: The morulae were stored with various concentrations of AFP type III for 24 h at 5, 15 and 25C. RESULTS: Supplementation of AFP type III (1...
March 2018: Cryo Letters
D M Pires, C D Corcini, A C de Silva, S Mm Gheller, F A Pereira, J R Pereira, J Re Muelbert, R D Jardim, J Re Garcia, A S Varela
  BACKGROUND: The cryopreservation protocol that has been developed exclusively for the preservation of the sperm of the species different. OBJECTIVE: this study was to evaluate the effect of the association of 10% DMSO with trehalose, raffinose, sucrose and lactose concentrations on the sperm cells of Piaractus mesopotamicus. MATERIALS AND METHODS: Sperms were collected from the animals through abdominal massage. The samples were diluted in the Beltsville Thawing Solution without different concentrations of other sugars (test conditions)...
March 2018: Cryo Letters
A Khellouf, K Benhenia, S Fatami, M Iguer-Ouada
BACKGROUND: During cryopreservation sperm cells suffer from two major deleterious impacts: oxidative stress and cold shock. OBJECTIVE: To investigate in bovine species the benefit of cholesterol and vitamin E, both loaded in cyclodextrins, as a double protection against oxidative stress and cold shock. MATERIALS AND METHODS: Semen was collected from nine mature bulls using an artificial vagina and each ejaculate was split into four equal aliquots...
March 2018: Cryo Letters
S S Sahraei, M Shahhoseini, B Movaghar
BACKGROUND: OBJECTIVE: This paper reports results from studies on modifications of histone marks in transcriptional regulatory regions of H19, Igf2 and Mest genes in vitrified-warmed and cultured mouse embryos. MATERIALS AND METHODS: Non vitrified and vitrified-warmed 8-cell embryos were cultured in Ham's F10+10% BSA into blastocyst stage in two experimental groups. Gene expression level was assessed by quantitative PCR and histone modifications were evaluated by ChIP assay method...
March 2018: Cryo Letters
O O Zaitseva, T V PoleZhaeva, A N Khudvakov, O N Solomina, V V Golovchenko
  BACKGROUND: Due to their valuable medicinal properties and high physiological activity, plant polysaccharides are currently being extensively studied. OBJECTIVE: The present study aims to investigate rauwolfian (pectin for Rauvolfia serpentina) supplementation on human leukocytes cryopreservation. We determined the сharacteristics of leukocytes undergoing freezing with pectin at different temperatures. METHODS: Donor leukocytes were frozen under the protection of comprehensive cryoprotectant solution and stored in electrical freezers (-20C, -40C, -80C)...
January 2018: Cryo Letters
H N Ferreira, J C Ferreira-Silva, J M Rocha, M C Farras, M Calixto, M T Moura, M A Alvarenga, A L Oliveira
BACKGROUND: Semen cryopreservation causes DNA damage, thus requiring continuous monitoring. OBJECTIVE: To compare two assays for sperm DNA fragmentation (SDF) from stallions with contrasting semen freezability. MATERIALS AND METHODS: Thirteen stallions were classified as good semen freezers (GSF) or bad semen freezers (BSF). Ejaculates were cryopreserved with three diluents. Semen was subject to SDF evaluation using the sperm chromatin structure assay (SCSA) and Halomax after thawing (0 h) and after a 4 h thermoresistance test...
January 2018: Cryo Letters
T Beule, P Ilbert, K Adeoti, T Durand-Gasselin, D Dumet, F Engelmann, F Morcillo
  BACKGROUND: A cryopreservation protocol has been established for oil palm somatic embryos (SEs), the efficiency of which must be evaluated, both in terms of regeneration and of long-term storage capacity, before its large-scale routine use. OBJECTIVE: To test the survival and recovery of 29 clones of oil palm somatic embryos cryostored for 20 years. MATERIALS AND METHODS: Clumps of SEs were pregrown for 7 days on medium containing 0.75 M sucrose, dehydrated in air-tight containers containing silica gel to moisture contents between 19-35% fresh weight, and then immersed directly in liquid nitrogen and stored in cryotanks for 20 years...
January 2018: Cryo Letters
J J Kim, Y K Nam, I C Bang, S P Gong
  BACKGROUND: Miho spine loach (Cobitis choii) is an endangered Korean endemic fish. Whole testis cryopreservation is a good way for species preservation, but needs to the sacrifice of a large number of fish to optimize the freezing condition. Considering this limitation, a surrogate fish species was used for the protocol development. OBJECTIVE: This study was to establish the effective condition for Miho spine loach whole testis cryopreservation by optimizing the conditions for whole testis cryopreservation in an allied species, mud loach (Misgurnus mizolepis)...
January 2018: Cryo Letters
M A Rahman, S H Park, I J Yu
  OBJECTIVE: The study evaluated the effects of glucose-fructose or sucrose supplementation in glycerol-free Tris (GFT) solution on motility, viability, the level of reactive oxygen species (ROS), as well as the level of apoptosis (BAX and BCL2) and motility (SMCP)-related gene expression of dog spermatozoa. MATERIALS AND METHODS: Spermatozoa (5×107 sperm/ml) were cryopreserved in GFT containing 86 mM glucose and 86 mM fructose (GF-GFT) or 100 mM sucrose (S-GFT). Progressive motility, viability, ROS (H2 O2 ) level and mRNA gene expression of spermatozoa were evaluated 0 h, 3 h or 6 h post-thaw at 24°C...
January 2018: Cryo Letters
J J Dai, J H Yang, S S Zhang, Y F Niu, Y N Chen, C F Wu, D F Zhang
  The survival of porcine oocytes is still very low after cryopreservation. OBJECTIVE: To investigate whether and when the mitochondrial function of vitrified porcine oocytes could be recovered post-thaw. MATERIALS AND METHODS: Mitochondrial potential, ROS level, ATP content, apoptotic rate, caspase activity, and parthenogenetics developmental ability of thawed porcine oocytes were measured after culture in vitro for 0, 1, 2 or 4 h. RESULTS: Mitochondrial potential after 2 h and 4 h post-thaw culture were 1...
January 2018: Cryo Letters
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