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Cryo Letters

D Yeni, M E Inanc, F Avdatek, P B Tuncer, B Cil, R Turkmen, U Tasdemir
BACKGROUND: Cryopreservation has a side effect on the motility, chromatin integrity and viability of sperm cells. OBJECTIVE: The present study investigated the effects of supplementation with rosmarinic acid (RA) Tris extender on sperm quality parameters, plasma and acrosome membrane damage, antioxidant enzyme activity and chromatin integrity following the freeze thawing process on bull spermatozoa. MATERIALS AND METHODS: Ejaculates were split into five aliquots and diluted to a final concentration of 15x106 spermatozoa per ml with the Tris extender containing RA (25, 50, 100 and 200 microgram per ml) and (control) and then frozen at a controlled rate...
March 2018: Cryo Letters
E J Hoekman, K E Tucker, C Am Jansen, C G Hilders
BACKGROUND: Oocyte vitrification is important for fertility preservation. There are debates, however, surrounding the components of the procedure itself. OBJECTIVE: before starting a vitrification program, we decided to (1) determine if blastocysts derived from previously frozen mouse embryos would be suitable to practice vitrification and (2) to analyze the factors that contributed to improving our "learning curve". MATERIALS AND METHODS: 58 expanded blastocysts cultured from commercially available frozen 1-cell mouse embryos (B6D2F1 x B6C3F1) were used...
March 2018: Cryo Letters
C Kumari, A Kumar, S K Sarangi, A Thirugnanam
BACKGROUND: Cryogen spray cooling is an effective method to treat nodular basal cell carcinoma. OBJECTIVE: To study the effect of spraying distance (z = 27 mm, 18 mm and 9 mm) on a nodular gel phantom and to validate the numerical model. MATERIALS AND METHODS: Liquid nitrogen is sprayed on agarose gel phantom using 0.8 mm nozzle diameter. A two-dimensional Pennes equation with phase change is solved on the axisymmetric non-orthogonal grid...
March 2018: Cryo Letters
T V Nguyen, F Tanihara, M Hirata, T Hirano, K Nishio, L T Kim Do, T V Nguyen, M Nii, T Otoi
BACKGROUND: Short-term storage is valuable method to reuse manipulated embryos. OBJECTIVE: The present study evaluated the effects of antifreeze protein (AFP) supplementation on the quality and development of in vitro-produced porcine morulae after short-term storage (24 h). MATERIALS AND METHODS: The morulae were stored with various concentrations of AFP type III for 24 h at 5, 15 and 25C. RESULTS: Supplementation of AFP type III (1...
March 2018: Cryo Letters
D M Pires, C D Corcini, A C de Silva, S Mm Gheller, F A Pereira, J R Pereira, J Re Muelbert, R D Jardim, J Re Garcia, A S Varela
  BACKGROUND: The cryopreservation protocol that has been developed exclusively for the preservation of the sperm of the species different. OBJECTIVE: this study was to evaluate the effect of the association of 10% DMSO with trehalose, raffinose, sucrose and lactose concentrations on the sperm cells of Piaractus mesopotamicus. MATERIALS AND METHODS: Sperms were collected from the animals through abdominal massage. The samples were diluted in the Beltsville Thawing Solution without different concentrations of other sugars (test conditions)...
March 2018: Cryo Letters
A Khellouf, K Benhenia, S Fatami, M Iguer-Ouada
BACKGROUND: During cryopreservation sperm cells suffer from two major deleterious impacts: oxidative stress and cold shock. OBJECTIVE: To investigate in bovine species the benefit of cholesterol and vitamin E, both loaded in cyclodextrins, as a double protection against oxidative stress and cold shock. MATERIALS AND METHODS: Semen was collected from nine mature bulls using an artificial vagina and each ejaculate was split into four equal aliquots...
March 2018: Cryo Letters
S S Sahraei, M Shahhoseini, B Movaghar
BACKGROUND: OBJECTIVE: This paper reports results from studies on modifications of histone marks in transcriptional regulatory regions of H19, Igf2 and Mest genes in vitrified-warmed and cultured mouse embryos. MATERIALS AND METHODS: Non vitrified and vitrified-warmed 8-cell embryos were cultured in Ham's F10+10% BSA into blastocyst stage in two experimental groups. Gene expression level was assessed by quantitative PCR and histone modifications were evaluated by ChIP assay method...
March 2018: Cryo Letters
O O Zaitseva, T V PoleZhaeva, A N Khudvakov, O N Solomina, V V Golovchenko
  BACKGROUND: Due to their valuable medicinal properties and high physiological activity, plant polysaccharides are currently being extensively studied. OBJECTIVE: The present study aims to investigate rauwolfian (pectin for Rauvolfia serpentina) supplementation on human leukocytes cryopreservation. We determined the сharacteristics of leukocytes undergoing freezing with pectin at different temperatures. METHODS: Donor leukocytes were frozen under the protection of comprehensive cryoprotectant solution and stored in electrical freezers (-20C, -40C, -80C)...
January 2018: Cryo Letters
H N Ferreira, J C Ferreira-Silva, J M Rocha, M C Farras, M Calixto, M T Moura, M A Alvarenga, A L Oliveira
BACKGROUND: Semen cryopreservation causes DNA damage, thus requiring continuous monitoring. OBJECTIVE: To compare two assays for sperm DNA fragmentation (SDF) from stallions with contrasting semen freezability. MATERIALS AND METHODS: Thirteen stallions were classified as good semen freezers (GSF) or bad semen freezers (BSF). Ejaculates were cryopreserved with three diluents. Semen was subject to SDF evaluation using the sperm chromatin structure assay (SCSA) and Halomax after thawing (0 h) and after a 4 h thermoresistance test...
January 2018: Cryo Letters
T Beule, P Ilbert, K Adeoti, T Durand-Gasselin, D Dumet, F Engelmann, F Morcillo
  BACKGROUND: A cryopreservation protocol has been established for oil palm somatic embryos (SEs), the efficiency of which must be evaluated, both in terms of regeneration and of long-term storage capacity, before its large-scale routine use. OBJECTIVE: To test the survival and recovery of 29 clones of oil palm somatic embryos cryostored for 20 years. MATERIALS AND METHODS: Clumps of SEs were pregrown for 7 days on medium containing 0.75 M sucrose, dehydrated in air-tight containers containing silica gel to moisture contents between 19-35% fresh weight, and then immersed directly in liquid nitrogen and stored in cryotanks for 20 years...
January 2018: Cryo Letters
J J Kim, Y K Nam, I C Bang, S P Gong
  BACKGROUND: Miho spine loach (Cobitis choii) is an endangered Korean endemic fish. Whole testis cryopreservation is a good way for species preservation, but needs to the sacrifice of a large number of fish to optimize the freezing condition. Considering this limitation, a surrogate fish species was used for the protocol development. OBJECTIVE: This study was to establish the effective condition for Miho spine loach whole testis cryopreservation by optimizing the conditions for whole testis cryopreservation in an allied species, mud loach (Misgurnus mizolepis)...
January 2018: Cryo Letters
M A Rahman, S H Park, I J Yu
  OBJECTIVE: The study evaluated the effects of glucose-fructose or sucrose supplementation in glycerol-free Tris (GFT) solution on motility, viability, the level of reactive oxygen species (ROS), as well as the level of apoptosis (BAX and BCL2) and motility (SMCP)-related gene expression of dog spermatozoa. MATERIALS AND METHODS: Spermatozoa (5×107 sperm/ml) were cryopreserved in GFT containing 86 mM glucose and 86 mM fructose (GF-GFT) or 100 mM sucrose (S-GFT). Progressive motility, viability, ROS (H2 O2 ) level and mRNA gene expression of spermatozoa were evaluated 0 h, 3 h or 6 h post-thaw at 24°C...
January 2018: Cryo Letters
J J Dai, J H Yang, S S Zhang, Y F Niu, Y N Chen, C F Wu, D F Zhang
  The survival of porcine oocytes is still very low after cryopreservation. OBJECTIVE: To investigate whether and when the mitochondrial function of vitrified porcine oocytes could be recovered post-thaw. MATERIALS AND METHODS: Mitochondrial potential, ROS level, ATP content, apoptotic rate, caspase activity, and parthenogenetics developmental ability of thawed porcine oocytes were measured after culture in vitro for 0, 1, 2 or 4 h. RESULTS: Mitochondrial potential after 2 h and 4 h post-thaw culture were 1...
January 2018: Cryo Letters
E E Benson, K Harding, M Ryan, A Petrenko, Y Petrenko, B Fuller
BACKGROUND: The development of encapsulation technologies has played an important role in improving cryopreservation outcomes for many cell and tissue types over the past 20 years. Alginate encapsulation cryopreservation (AECryo) has been incorporated into a range of applications in biotechnology, species conservation and clinical therapies, using cells from many different phyla, including higher plants, animal and human cells. This review describes the background to the origins of AECryo, the development of AECryo in higher plant tissues, broadening to current applications in algal conservation, the roles for AECryo in preserving phytodiversity, fungal species and in animal and human cells...
January 2018: Cryo Letters
K Memon, Y Cheng, F Panhwar, Z R Chen, Z Haider, S Afridi, P Hu, G Zhao
  BACKGROUND: Osmotic shock upon the addition and removal of cryoprotectant agent (CPA) is a major source of cell damage during cryopreservation. OBJECTIVE: Microfluidic device offers a new platform for CPA loading and unloading. The micro scale dimension makes possible to perform a detailed analysis and controllable removal of CPA with many advantages. MATERIALS AND METHODS: A microfluidic device was developed for extracting dimethyl sulfoxide (DMSO) from the sample streamline...
January 2018: Cryo Letters
Y Wang, X M Zhou, C J Jiang, Y T Yu
BACKGROUND: The microchannel heat exchange system has several advantages and can be used to enhance heat transfer for vitrification. OBJECTIVE: To evaluate the microchannel cooling method and to analyze the effects of key parameters such as channel structure, flow rate and sample size. MATERIALS AND METHODS: A computational flow dynamics model is applied to study the two-phase flow in microchannels and its related heat transfer process. The fluid-solid coupling problem is solved with a whole field solution method (i...
January 2018: Cryo Letters
M I Serqushkina, A N Khudyakov, T V Polezhaeva, O M Bezmeltseva
BACKGROUND: Pectins have a large number of active -OH and -COOH groups (3), suggesting the presence of this group of substances specific cryoprotective effect. OBJECTIVE: This research is aimed at studying the ability of pectins (zosteran, apple pectin АU-701, E-440) to change the freezing temperature of glycerol, which can increase the effect in the cryopreservation of nuclear cells. METHODS: Using the cryoscopic method we studied the ability of pectins (zosteran, apple pectin AU-701, E-440) to change the freezing temperature of glycerol...
November 2017: Cryo Letters
S A Lone, T K Mohanty, M Bhakat, R K Baithalu, R Kumar
  BACKGROUND: Sperm concentration needed for artificial insemination (AI) to obtain reasonable fertility, taking into consideration genetic value of bull and numerous others components is one of the essential constituents for successful breeding program. AI-doses containing low sperm numbers are increasingly widespread to optimize the benefit of elite bulls, as well as to accommodate an eventual wider application of sex-sorted semen. Viability is reduced when higher dilutions of semen are carried out for production of low sperm doses...
November 2017: Cryo Letters
A Karun, K K Sajini, K S Muralikrishna, M K Rajesh, F Engelmann
BACKGROUND: Cryopreservation opens new avenues in the field of genetic resource conservation, especially in recalcitrant seeded palms such as arecanut for which field genebanks are exposed to pest and disease attacks and natural calamities. It is only through cryopreservation that the safety of the conserved germplasm can be assured at a relatively low cost for extended periods. OBJECTIVE: The objective of this work was to standardize various aspects of arecanut pollen cryopreservation, viz...
November 2017: Cryo Letters
H S Youm, J R Choi, D Oh, Y H Rho
BACKGROUND: T he most commonly used methods for the cryopreservation of oocytes and embryos are vitrification and slow freezing. OBJECTIVE: The aim of this study was to to investigate whether there are differences in survival, in vitro maturation (IVM), and fertilization rates between cryopreserved immature oocytes, especially germinal vesicle (GV)-stage human oocytes, following vitrification and slow freezing. MATERIALS AND METHODS: A literature search was performed using the MEDLINE, Cochrane Central Register of Controlled Trials, and Embase databases...
November 2017: Cryo Letters
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