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Cryo Letters

Bin Tian, Lili Xu, Miao Zhang, Yuqian Feng, Shixiang Zong
BACKGROUND: Holcocerus hippophaecolus is the most serious pest occurred in seabuckthorn forest of three north areas. OBJECTIVE: The primary aims of the current study were to explore the physiological mechanisms and adaptability of H. hippophaecolus to low temperatures. MATERIALS AND METHODS: Assessing supercooling point, freezing point, and cryoprotectants of different larval instars from three different populations. RESULTS: Supercooling capacity of larvae from the 8-13 instar groups was relatively independent of temperature and other indicators such as latitude...
May 2016: Cryo Letters
T B Sridharan, A S Vickram
Cryopreservation is a technique by which, semen can be preserved to subzero temperature, usually at -196° C. The freezing of semen desires vitrification mediators that diminish wreck to the cells (spermatozoan) during the freeze and thaw process. Using cryopreservation, the quality of the semen has been increased in the latest years, by which the achievement rate for the insemination techniques has increased in an agreed way. The area need to be focused is to enhance the quality of the semen extender preparation before cryopreservation...
May 2016: Cryo Letters
Yingfang Niu, Jianjun Dai, Yaning Chen, Caifeng Wu, Shushan Zhang, Defu Zhang
BACKGROUND: The developmental potential of vitrified porcine oocytes is very lower, and apoptosis is considered as one of the key factors involved. OBJECTIVE: To investigate the effects of apoptotic inhibitor Z-VAD-FMK addition into the incubation medium after warming on apoptosis and developmental ability of vitrified porcine MII-stage oocytes. MATERIALS AND METHODS: The activities of several caspases, mitochondrial membrane potential (ΔΨm) and early apoptotic levels were measured...
May 2016: Cryo Letters
Anna Misianiková, Daniela Zubrická, Linda Petijová, Katarina Brunákova, Eva Cellárová
BACKGROUND: The increasing demand for hypericins and hyperforins, the unique pharmaceuticals found in the Hypericum genus, requires the development of effective tools for long-term storage of cells and tissues with unique biochemical profiles. OBJECTIVE: To determine the temperature of crystallization (T(C)) and of ice formation of 14 cryoprotectant mixtures (CMs) for their use in cryoprotection of H. perforatum L. cell suspensions and to evaluate the impact of the lowest Tc on post-cryogenic recovery...
May 2016: Cryo Letters
Monika Höfer
BACKGROUND: The National German Strawberry Genebank, which includes 183 cultivars and 270 accessions of wild Fragaria species, is maintained in a field collection of the Fruit Genebank at Dresden-Pillnitz. OBJECTIVE: A duplicate collection stored in liquid nitrogen would provide a higher level of security for these irreplaceable genetic resources. MATERIALS AND METHODS: Four distinct cryopreservation protocols were tested earlier and the best method, a PVS2 vitrification method with a 14-day alternating-temperature cold acclimation, was modified for further genotype screening...
May 2016: Cryo Letters
Supornchai Chaireok, Kanchit Thammasiri, Upatham Meesawat
BACKGROUND: Paphiopedilum niveum (Rchb.f.) Stein, an endangered lady's slipper orchid, is listed in CITES Appendix I and thus requires conserving. OBJECTIVE: The study aimed to cryopreserve protocorm-like bodies (PLBs) by a vitrification technique. MATERIALS AND METHODS: Four-month-old PLBs were precultured in modified Vacin and Went liquid medium with various sucrose concentrations for 24 h and 5 d with daily increasing sucrose concentrations followed by different exposure times to plant vitrification solution 2 (PVS2)...
May 2016: Cryo Letters
Xiaorong Huang, Guangpeng Feng, Feng Zhao, Jianyi Liu, Tao Zhang, Yu Wang, Ping Zhuang
BACKGROUND: Vitrification is the most promising option for the cryopreservation of fish embryos but requires high concentrations of potentially toxic cryoprotectants that can also cause cell injury, and affect cell division, enzymatic activities and cell metabolism. The effect of cryopreservation on the enzyme activity in crustacean embryos has not been reported. OBJECTIVES: The aim of this study was to investigate the effects of a vitrification protocol on the enzyme activity of different stages of Eriocheir sinensis embryos...
May 2016: Cryo Letters
H Zhang, Y Yang, W Ma, H Wu, X Zheng, C Hei, M Sun, W Ma, H Ma, Q Chang, H Wang, Y Cai, Yan Xie, C Zhao, X Pei, Y Wang
BACKGROUND: Ovarian cryopreservation by vitrification is a very effective pathway for the preservation of female fertility during radiotherapy and chemotherapy. However, damage of follicles was triggered by cryo-injure during the process of ovarian vitrification and ischemia/reperfusion during the process of ovarian transplantation. Appropriate FSH play important roles in anti-apoptosis and neoangiogenesis during ovarian follicle development. OBJECTIVE: Therefore, the purpose of this study was to investigate the effect of FSH on the revascularization and follicular survival of vitrified-warmed ovarian grafts...
March 2016: Cryo Letters
E E Uchendu, E R Joachim Keller
BACKGROUND: The cryopreservation of yam is constrained with many challenges. OBJECTIVE: This study tested the effects of melatonin on shoot tips of D. alata and D. cayenensis accessions exposed to water and liquid nitrogen (LN) stresses. MATERIALS AND METHODS: Sucrose pretreatment (0.3 M) was applied for 48 h before cryopreservation. Shoot tips were encapsulated in beads loaded with 0.75 M sucrose, with and without melatonin and desiccated over sterile dry silica gel for 0 - 9 h...
March 2016: Cryo Letters
L B Cordova, K Thammasiri
BACKGROUND: There are various methods for the cryopreservation of plant material, with each biological specimen potentially requiring protocol optimization to maximize success. OBJECTIVE: The aim of this study is to compare droplet-vitrification, encapsulation-dehydration, and the cryo-plate method for cryopreservation of protocorms of the orchid Arundina graminifolia, using silica gel and drying beads as the desiccation materials. MATERIALS AND METHODS: The cryo-plate method included preculture of protocorms, developed from seeds, placed on aluminium cryo-plates and embedded in alginate gel...
March 2016: Cryo Letters
Md A Rahman, S H Park, I J Yu
OBJECTIVE: The present study was to investigate a freezing method using one step-dilution with glycerol-free TRIS extender containing 172.2 mM glucose (GFTG). MATERIALS AND METHODS: The sperm pellet from selected ejaculates was resuspended in GFTG at 1×10(8) cells/mL. The semen was cooled for 10, 30, 50 or 70 min in GFTG at 4 degree C and was frozen in LN(2) vapor or in deep freezer (-80 degree C, DF) for 20 min before plunge into LN(2). Post-thaw sperm characteristics were examined...
March 2016: Cryo Letters
J J Dai, Y F Niu, C F Wu, S H Zhang, D F Zhang
BACKGROUND: Oocytes vitrification is widely used for cryopreservation of female genetic resources. OBJECTIVE: In order to illuminate the apoptotic pathways of porcine MII stage oocytes after vitrification. MATERIALS AND METHODS: This study used in situ fluorescence staining and RT-PCR to detect the expression levels of some key molecules from death receptor and mitochondria mediated apoptotic pathways. RESULTS: (1) Early stage apoptosis were detected in both PI staining survival oocytes and PI staining dead oocytes...
March 2016: Cryo Letters
J O Daramola, E O Adekunle
BACKGROUND: Slow freezing and vitrification are used to improve the viability of spermatozoa from various species but comparative effects of these cryoprotocols have never been evaluated for spermatozoa obtained from West African Dwarf (WAD) goat bucks. OBJECTIVE: This study evaluated the comparative effects of slow freezing and vitrification on the viability of spermatozoa of WAD goat bucks. MATERIALS AND METHODS: Semen samples collected with the aid of artificial vagina were allocated to slow freezing and vitrification protocols and cryopreserved for 30 days in liquid nitrogen...
March 2016: Cryo Letters
X J Chen, Y Zhang, G X Jia, Q G Meng, T D Bunch, G S Liu, S E Zhu, G B Xhou
BACKGROUND: Antioxidants protect spermatozoa against cell damage during cryopreservation. OBJECTIVE: To investigate whether melatonin supplement in the extender may improve the quality of cryopreserved mouse sperm. METHODS: Kunming mice sperm frozen in extender R18S3 (18% (w/v) raffinose and 3% (w/v) skim milk) supplemented with melatonin were thawed and evaluated. RESULTS: Mouse spermatozoa were cryopreserved in the freezing extender R18S3 that contained melatonin at 0, 0...
March 2016: Cryo Letters
A Meyer, A L Charles, F Singh, J Zoll, S Talha, I Enache, A Chaarloux, M E Inser-Horobeti, B Geny
BACKGROUND: Cardiac muscle cryopreservation is a challenge for both diagnostic procedure requiring viable tissues and therapeutic advance in regenerative medicine. Mitochondria are targets of both direct and indirect damages, secondary to congelation per se and/or to cryoprotectant's toxic effects, which participate to diminution of viability and/or functioning of cells after freezing. At the cardiac muscle level, only one study had investigated mitochondrial respiration after cryopreservation...
March 2016: Cryo Letters
V H Nguyen, V L Nguyen, N S Hoang, T K Do
BACKGROUND: The discovery of proteins with inherent cell membrane-translocating activity will expand our ability to study and manipulate various intracellular processes in living systems. OBJECTIVE: We investigated the effect of TAT-EGFP (trans-activator of transcription-enhanced green fluorescent protein) intra-cellular delivery on the survival and development of mature porcine oocytes after cryopreservasion. MATERIALS AND METHODS: Cumulus-oocyte complexes (COCs) collected from follicles 3 to 6 mm in diameter in abattoir-derived oocytesries of prepubertal gilts were on vitro matured (IVM)...
March 2016: Cryo Letters
In-Sul Hwang, D ae-Jin Kwon, Tae-Uk Kwak, Jeong-Woong Lee, Gi-Sun Im, Seongsoo Hwang
BACKGROUND: The primary problems with porcine oocyte vitrification are their low viability and development; both need improvement. OBJECTIVE: This study was designed to improve the survival and developmental rates in vitrified-warmed porcine oocytes. MATERIALS AND METHODS: Porcine oocytes matured in vitro were vitrified-warmed with Cryotop. Then the oocytes were supplemented with Q10 during recovery culture. RESULTS: The survival rates immediately after warming were 92...
January 2016: Cryo Letters
Xiaoming Zhou, Xuliang Zhang, Ying Zhang, Shuqing Hong, Lin Li, Zhong Liu
BACKGROUND: Previous studies have demonstrated that human RBCs can survival from freeze-drying, but there is still some uncertainty on whether and how the cell properties change during the processes. OBJECTIVE: The present study is to evaluate the effects of lyophilization and rehydration on the membrane surface antigens, such as CD antigens and blood group antigens, of human RBCs. MATERIALS AND METHODS: Human RBCs were lyophilized first through a simplified protocol, in which glycerol were used in the lyoprotectant solution instead of trehalose...
January 2016: Cryo Letters
X Garcia-Dominguez, C D Vera-Donoso, E Jimenez-Trigos, J S Vicente, F Marco-Jimenez
BACKGROUND: Kidney transplantation from deceased or living human donors has been limited by donor availability as opposed to the increasing demand, by the risks of allograft loss rejection and immunosuppressive therapy toxicity and by limitations of organ preservation protocols, which is essential to organise staff and facilities, transport organs, and perform necessary laboratory tests. However, the cryopreservation of composite tissues poses technical challenges beyond those seen in the preservation of single tissue types or organs...
January 2016: Cryo Letters
Filiz Kutluyer, Fatih Öğretmen, Burak Evren Inanan
BACKGROUND: Amino acids, present in seminal plasma at high concentration, protect spermatozoa against cell damage during cryopreservation. OBJECTIVE: Experiments were designed to analyze the effect of semen extender supplemented with taurine on post-thawed sperm motility and duration, as well as DNA damage. MATERIALS AND METHODS: Extenders were supplemented with 1, 2 or 4 mM taurine. Semen samples were diluted at the ratio of 1:9 with the extenders...
January 2016: Cryo Letters
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