journal
MENU ▼
Read by QxMD icon Read
search

Cryo Letters

journal
https://www.readbyqxmd.com/read/28072433/effects-of-superparamagnetic-nanoparticles-on-nucleation-and-crystal-growth-in-the-vitrified-vs55-during-warming
#1
Y Xu, H M Yu, Y Q Niu, S C Luo, X Cheng
:   BACKGROUND:Magnetic nanoparticles (mNPs), once excited by radiofrequency (RF) energy, could heat uniformly and rapidly the vitrified biospecimens. However, there are few studies about the impact of mNPs on crystallization kinetics of vitrified samples. OBJECTIVES: The present work aims to investigate the nucleation and crystal growth in the vitrification solution VS55 with mNPs. MATERIALS AND METHODS: Ferrotec EMG308 superparamagnetic nanoparticles (10 ± 2...
November 2016: Cryo Letters
https://www.readbyqxmd.com/read/28072432/closed-vitrification-system-as-a-platform-for-cryopreservation-of-tissue-engineered-constructs
#2
N A Trufanova, V S Zaikov, A V Zinchenko, A Yu Petrenko, Y A Petrenko
BACKGROUND: Cryopreservation of mesenchymal stromal cells (MSCs) and MSCs-based tissue engineered constructs (TECs) is a promising strategy for regenerative medicine. OBJECTIVE: To examine vitrification system consisting of multicomponent vitreous solution, closed type container, human adult MSCs and two-step exposure procedure as a platform for cryopreservation of MSCs-based TECs. MATERIALS AND METHODS: Vitrification properties of solutions were studied by visual analysis and calorimetry...
November 2016: Cryo Letters
https://www.readbyqxmd.com/read/28072431/expression-patterns-of-three-genes-under-short-and-long-term-cold-exposure-in-thitarodes-pui-lepidoptera-hepialidae-a-host-of-ophiocordyceps-sinensis
#3
Q Min, S Y Cheng, J F Xi, J Ma, T R Xin, B Xia, Z W Zou
:   BACKGROUND: Thitarodes larvae are the host of the caterpillar fungus Ophiocordyceps sinensis. Low temperature is the main environmental limitation for larvae growth. OBJECTIVE: To better understand the cold adaption process in T. pui larvae, the expression patterns of trehalose-6-phosphate synthase (TpTPS), heat shock protein 70 (TpHSP70), and heat shock protein 90 (TpHSP90) were investigated upon short and long-term exposure to 0°C. MATERIALS AND METHODS: The 6th instar T...
November 2016: Cryo Letters
https://www.readbyqxmd.com/read/28072430/measurement-of-thermal-conductivity-of-porcine-liver-in-the-temperature-range-of-cryotherapy-and-hyperthermia-250-315k-by-a-thermal-sensor-made-of-a-micron-scale-enameled-copper-wire
#4
Z D Jiang, G Zhao, G R Lu
:   BACKGROUND: Cryotherapy and hyperthermia are effective treatments for several diseases, especially for liver cancers. Thermal conductivity is a significant thermal property for the prediction and guidance of surgical procedure. However, the thermal conductivities of organs and tissues, especially over the temperature range of both cryotherapy and hyperthermia are scarce. OBJECTIVE: To provide comprehensive thermal conductivity of liver for both cryotherapy and hyperthermia...
November 2016: Cryo Letters
https://www.readbyqxmd.com/read/28072429/integrating-micro-or-nanoscale-encapsulation-technology-with-vitreous-cryopreservation-a-new-strategy-to-improve-biopreservation
#5
Y Y Zheng, G Zhao
:   BACKGROUND: None-uniform distributions of temperatures, limited freezing and thawing rates, and thermal stresses are three main hindering factors for successful vitreous cryopreservation of mass volume of biosamples. Micro- and nanoscale encapsulation, owning the intrinsic features to avoid those limitations introduced by the traditional approaches, has been opened up a new way for effective and high-efficiency biopreservation. NEW APPROACH: This short review article summarizes recent advances in cell encapsulation technology for biopreservation by manipulating cells and biological agents in micro- or nanoscale volume droplets and microgels, and discusses its promising applications for future vitreous cryopreservation...
November 2016: Cryo Letters
https://www.readbyqxmd.com/read/28072428/3d-modelling-on-biodegradable-nanoparticle-enhanced-cryoablation-of-liver-tumor-based-on-real-anatomical-model
#6
S Y Xu, Z Z He, Y X Zhou, J Liu
BACKGROUND: Nanoparticle-enhanced freezing is of great importance for developing a conformal targeted cryoablation for liver tumor with complex shape. However, the safety and biocompatibility of nanoparticles should also be of major concerns. OBJECTIVE: This study is to investigate the enhanced cryoablation mediated by the MgO nanoparticles which are nontoxic, biodegradable, and have few side-effects on the human body. MATERIALS AND METHODS: A three-dimensional numerical model has been developed based on a real geometrical anatomical structure to characterize such nanocryosurgical freezing of liver tumor...
November 2016: Cryo Letters
https://www.readbyqxmd.com/read/28072427/calorimetric-studies-on-thermal-properties-of-nano-cryoprotectant-solutions-during-vitrification
#7
H F Xu, B T Hao, L J Liu, L L Tang, B L Liu
:   BACKGROUND: Vitrification, the ice-free cryopreservation, develops rapidly and can become an ideal method for long-term preservation of cells and tissues. But up to now it is not practical for samples with large size because of the ultra-rapid cooling rate required. It has been reported that nanoparticles improve heat conductivity of solutions. MATERIALS AND METHODS: In this study, Hydroxyapatite (HA) nanoparticles(20, 40 or 60nm)at 0.1 %, 0.5 % or 1 % (w/w) were added into glycerol solutions...
November 2016: Cryo Letters
https://www.readbyqxmd.com/read/28072426/effect-of-nanoparticles-on-the-survival-and-development-of-vitrified-porcine-gv-oocytes
#8
W J Li, X L Zhou, B L Liu, J J Dai, P Song, Y Teng
:   BACKGROUND: Some mammalian oocytes have been successfully cryopreserved by vitrification. However, the survival and developmental rate of vitrified oocytes is still low. The incorporation of nanoparticles into cryoprotectant (CPA) may improve the efficiency of vitrification by changing the properties of solutions. MATERIALS AND METHODS: The toxicity of different concentrations of hydroxy apatite (HA), silica dioxide (SO2), aluminum oxide (Al2O3) and titanium dioxide (TiO2) nanoparticles (20 nm in diameter) to oocytes was tested and the toxicity threshold value of each nanoparticle was determined...
November 2016: Cryo Letters
https://www.readbyqxmd.com/read/28072425/direct-microscale-measurement-of-mouse-oocyte-membrane-permeability-to-water-and-ethylene-glycol-at-subzero-temperatures-using-cryomicroscopy
#9
X Han
:   BACKGROUND: Investigation of cell osmotic behavior at subzero temperatures is of critical importance to the optimization of cooling procedures for cryopreservation. Based on established thermodynamic models, plasma membrane permeability coefficients for water and cryoprotectant agent (CPA) (Lcpa, Pp) and their activation energies (Ea(Lp), Ea(Pcpa)) are essential to predict the change of cell volume and composition of intracellular solutions corresponding to different cooling procedures...
November 2016: Cryo Letters
https://www.readbyqxmd.com/read/28072424/a-theoretical-and-experimental-investigation-of-mechanical-damage-to-rodent-sperm-generated-by-microscale-ice-formation
#10
X Han, J K Critser
:   BACKGROUND: Rodent sperm cryopreservation is of critical importance for the maintenance of lines or strains of genetically engineered mice and rats. However, rodent sperm are extremely mechanically sensitive due to their unusual morphology, and are severely damaged using current methods of cryopreservation. Those methods result in poor post thaw motility (PTM) for mouse. OBJECTIVE: To investigate the mechanism of mechanical damage introduced to rodent sperm during freezing, a micro-mechanical model was established to analyze the sperm radial and axial thermal stresses generated by microscale extracellular ice formation...
November 2016: Cryo Letters
https://www.readbyqxmd.com/read/28072423/nanotechnology-based-cryopreservation-of-cell-scaffold-constructs-a-new-breakthrough-to-clinical-application
#11
G Chen, Y Lv
  The developments of "off-the-shelf" cell-scaffold constructs received an increasing interest in tissue engineering and regenerative medicine. Although the direct cryopreservation of a single-cell suspension in the tube is a relative mature technology, the cryopreservation of cell-scaffold constructs remains a challenge. Nanotechnology shows tremendous potential for cryopreservation in regulating of freezing and thawing processes. For example, nanoparticles have been reported to modify the cryoprotective agent (CPA), adjust the process of cooling and warming cycles...
November 2016: Cryo Letters
https://www.readbyqxmd.com/read/27925004/appropriate-osmotic-balance-duration-for-different-volumes-of-ovarian-tissue-in-vitrification-solution-a-study-of-ovary-tissue-vitrification-and-transplantation-in-sheep
#12
L Dang, X Zheng, Q Chang, Y Yang, Y Wang, Y Cai, C Hei, H Wang, C Zhao, W Zhu, Y Wang
:   BACKGROUND: Auto-transplantation of cryopreserved ovarian tissue has become a promising method for fertility preservation and standardization of the process is crucial for practical applications. OBJECTIVE: Here we used different size of large sheep ovarian cortex to study the most suitable osmotic balance durations in the vitrification solution for large piece ovary cortex. MATERIALS AND METHODS: The ovarian cortices from six-month old female sheep were divided into 40 cubic mm, 80 cubic mm and 160 cubic mm volume, A two-step osmotic balance method was used based on the results from morphological and histological study, we detected the expression of VEGF after thawing, the percentage of follicles that survived and serum E2 levels,together with apoptosis test by TUNEL...
September 2016: Cryo Letters
https://www.readbyqxmd.com/read/27925003/impairment-of-barrier-properties-of-erythrocyte-membranes-caused-by-low-temperatures-is-a-result-of-disorganization-of-hemoglobin-supramolecular-structure
#13
A K Gulevskvy, N V Repin, I I Schenvavsky
BACKGROUND: The antecedence of impairment of plasmatic membrane structure and functions forms the basis of the dominative concept about mechanisms of cell cryoinjuries. A role of alterations of hemoglobin supramolecular structure in erythrocytes remains unclear. OBJECTIVE: Comparison of continuity of membranes of native erythrocytes and resealed ghosts after freeze-thawing with a cryoprotectant at a low concentration (4%). MATERIALS AND METHODS: Cryoresistance of native erythrocytes and resealed ghosts with and without low concentrations of cryoprotectants (4% glycerol) was compared according to egress of the following markers: hemoglobin, 14С-sucrose and K+ as well as by scanning electron microscopy...
September 2016: Cryo Letters
https://www.readbyqxmd.com/read/27925002/a-novel-adjuvant-solution-layer-strategy-for-improving-the-efficacy-of-cryosurgery
#14
K K Ramajayam, A Kumar, S K Sarangi, A Thirugnanam
BACKGROUND: During cryosurgery, studies reported earlier suggest increased destruction inside the tumour due to adjuvants or the prevention of damage to neighbouring healthy tissue through different methods. OBJECTIVE: This study advocates a novel strategy that increases the freezing inside agarose gel phantoms by addition of glycine and limits the freezing to the desired location using a perfluorohexane layer during cryosurgery. MATERIALS AND METHODS: Cryosurgery of glycine containing gels is carried out with and without perfluorohexane layer and the thermal history is measured using K-type thermocouples connected to a data acquisition system...
September 2016: Cryo Letters
https://www.readbyqxmd.com/read/27925001/dehydration-preparation-of-mouse-sperm-for-vitrification-and-rapid-laser-warming
#15
E Paredes, P Mazur
BACKGROUND: Mice are fundamental models of study due to their ease of breeding, manipulation, and the well-studied genome. There has been extensive research focused on the cryopreservation of mouse germaplasm, as a way to help maintain the different transgenic mouse breeds. The first protocols for mouse sperm were developed in the 90's using slow cooling and a mixture of raffinose and glycerol. Since then, the rate of success reported remains highly variable. OBJECTIVE: The Aim of this work is to study factors that are key for developing vitrification protocols for ultra-rapid laser warming of mouse sperm...
September 2016: Cryo Letters
https://www.readbyqxmd.com/read/27925000/optimization-of-artificial-propagation-in-piracanjuba-fish-brycon-orbignyanus-using-cryopreserved-semen
#16
V O Felizardo, C C V Melo, L D S Murgas, E S Andrade, R D Navarro, T F Ftreitas
:   BACKGROUND: Cryopreserved semen could facilitate procedures during the artificial reproduction in fish. Factors affecting cryopreservation efficiency are important to define efficient protocols. OBJECTIVE: This study investigated the application of cryoprotectants on the quality of piracanjuba fish semen, the sperm concentration required for oocyte fertilization and spermatic activation. MATERIALS AND METHODS: We evaluated two intracellular cryoprotectant solutions (DMSO and methanol) and two extracellular cryoprotectant solutions (egg yolk and lactose) to cryopreserved piracanjuba semen...
September 2016: Cryo Letters
https://www.readbyqxmd.com/read/27924999/the-potato-cryobank-at-the-international-potato-center-cip-a-model-for-long-term-conservation-of-clonal-plant-genetic-resources-collections-of-the-future
#17
R Vollmer, R Villagaray, V Egusquiza, J Espirilla, M García, A Torres, E Rojas, A Panta, N A Barkley, D Ellis
BACKGROUND: Cryobanks are a secure, efficient and low cost method for the long-term conservation of plant genetic resources for theoretically centuries or millennia with minimal maintenance. OBJECTIVE: The present manuscript describes CIP's modified protocol for potato cryopreservation, its large-scale application, and the establishment of quality and operational standards, which included a viability reassessment of material entering the cryobank. MATERIALS AND METHODS: In 2013, CIP established stricter quality and operational standards under which 1,028 potato accessions were cryopreserved with an improved PVS2-droplet protocol...
September 2016: Cryo Letters
https://www.readbyqxmd.com/read/27924998/optimization-of-water-content-for-the-cryopreservation-of-allium-sativum-in-vitro-cultures-by-encapsulation-dehydration
#18
P T Lynch, G R Souch, J Zamecnik, K Harding
BACKGROUND: There is a general requirement to determine and correlate water content to viability for the standardization of conservation protocols to facilitate effective cryostorage of plant germplasm. OBJECTIVE: This study examined water content as a critical factor to optimize the cryostorage of Allium sativum. MATERIALS AND METHODS: Stem discs were excised from post-harvest, stored bulbs prior to cryopreservation by encapsulation-dehydration and water content was determined gravimetrically...
September 2016: Cryo Letters
https://www.readbyqxmd.com/read/27924997/characterization-of-normal-and-freeze-thawed-tissues-in-vitro-through-the-ultrasonic-integrated-backscatter
#19
L Sheng, P Yang, Z He, G Wang, J Luo, J Liu
BACKGROUND: The ultrasound guidance of cryotherapy could be improved if changes of tissue characterization parameters during freezing could be used to monitor freezing patterns and estimate the postoperative effects after surgery. OBJECTIVE: The ultrasonic integrated backscatter analysis holds promise as an effective method for signal analysis and characterization of thawed tissues. MATERIALS AND METHODS: The ultrasonic integrated backscatter has been found to be an important parameter for describing the ultrasonic scattering and characterization of biological tissues...
September 2016: Cryo Letters
https://www.readbyqxmd.com/read/27925012/effect-of-nac-on-mouse-gv-oocyte-survival-and-subsequent-embryonic-development-following-vitrfication
#20
S L Yue, Y T Zhang, S W Wang, M Sun, Y C Xing, J Wen, J B Zhou
BACKGROUND: Oocytes that survive cryopreservation may accumulate ROS which are known to bring harmful effects on embryonic development. NAC is an antioxidant which can be a supplement to reduce oxidative stress. However, whether NAC can improve the developmental competence of vitrified GV-oocytes remains unclear. OBJECTIVE: The study was to investigate the effect of NAC on subsequent embryonic developmental competence of mice vitrified GV-oocytes. MATERIALS AND METHODS: This study compared the effects of different concentration of NAC on the cleavage and blastocyst rates of mice vitrified GV-oocytes...
July 2016: Cryo Letters
journal
journal
34428
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"