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Bioresource Technology

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https://www.readbyqxmd.com/read/29161572/new-%C3%AE-galactosidase-producers-with-potential-for-prebiotic-synthesis
#1
Sara C Silvério, Eugénia A Macedo, José A Teixeira, Lígia R Rodrigues
β-Galactosidases (EC 3.2.1.23) are interesting enzymes with potential application in the pharmaceutical and food industry. In this work, a screening study was carried out to identify new fungal sources of β-galactosidase. A total of 50 fungi were evaluated using a chromogenic test performed in agar plates. The most promising microorganisms were validated as effective β-galactosidase producers under submerged fermentation conditions. The crude β-galactosidases were characterized regarding their optimal pH (3...
November 15, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29161574/fast-spectroscopic-monitoring-of-inhibitors-in-the-2g-ethanol-process
#2
Ariane S S Pinto, Marcelo P A Ribeiro, Cristiane S Farinas
One of the main challenges of second generation (2G) ethanol production is the high quantities of phenolic compounds and furan derivatives generated in the pretreatment of the lignocellulosic biomass, which inhibit the enzymatic hydrolysis and fermentation steps. Fast monitoring of these inhibitory compounds could provide better control of the pretreatment, hydrolysis, and fermentation processes by enabling the implementation of strategic process control actions. We investigated the feasibility of monitoring these inhibitory compounds by ultraviolet-visible (UV-Vis) spectroscopy associated with partial least squares (PLS) regression...
November 14, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29161570/acclimation-of-acid-tolerant-methanogenic-propionate-utilizing-culture-and-microbial-community-dissecting
#3
Ying Li, Yongming Sun, Lianhua Li, Zhenhong Yuan
The acid-tolerant methanogenic propionate degradation culture was acclimated in a propionate-fed semi-continuous bioreactor by daily adjusting the digestate pH. The performance of propionate fermentation, the respond of microbial community structure to the acidic environment, and the microbial network for propionate degradation in the acid-tolerant culture was investigated. The results demonstrated that after long term of acclimation to low pH, the digester could produce methane from propionate at pH 4.8-5...
November 13, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29161571/effects-of-inorganic-carbon-on-the-nitrous-oxide-emissions-and-microbial-diversity-of-an-anaerobic-ammonia-oxidation-reactor
#4
Wenjie Zhang, Dunqiu Wang, Yue Jin
Inorganic carbon (IC) is important for anaerobic ammonium oxidation (anammox). In this study, the effects of the IC concentration on N2O emissions and microbial diversity in an anammox reactor were investigated. N2O emissions were positively correlated with IC concentrations, and IC concentrations in the range of 55-130 mg/L were optimal, considering the nitrogen removal rate and N2O emissions. High IC concentrations resulted in the formation of CaCO3 on the surface of anammox granules, which impacted the diffusion conditions of the substrate...
November 11, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29161569/denitrification-of-landfill-leachate-under-different-hydraulic-retention-time-in-a-two-stage-anoxic-oxic-combined-membrane-bioreactor-process-performances-and-bacterial-community
#5
Jianbo Liu, Panyue Zhang, Haibo Li, Yuan Tian, Siyu Wang, Yonghui Song, Guangming Zeng, Chen Sun, Zhiyong Tian
Two-stage anoxic/oxic combined membrane bioreactor (A/O-A/O-MBR) process was used to treat leachate generated from Shenyang Laohuchong landfill, and the effect of hydraulic retention time (HRT) was studied. A long HRT of 9 d and a short HRT of 5 d showed negative effect on the stability of process, resulting in a higher organic concentration of effluent than that with a HRT of 7 d, while the highest removal of chemical oxygen demand (COD), ammonia (NH4(+)-N) and total nitrogen (TN) were achieved with a HRT of 7 d, which was 82...
November 11, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29161568/feasibility-of-high-concentration-cellulosic-bioethanol-production-from-undetoxified-whole-monterey-pine-slurry
#6
Chengyu Dong, Ying Wang, Han Zhang, Shao-Yuan Leu
The economic feasibility of high-concentration cellulosic bioethanol production remains challenging because it requires easily available feedstock and low energy consumption process. Simultaneous saccharification and fermentation (SSF) of sulfite pretreated Momentary pine slurry at 20% (w/w) loadings increased ethanol concentration from 59.3 g/L to 68.5 g/L by washing strategy. Effects of inhibitors in pretreatment liquor were further investigated. Besides HMF, furfural and acetic acid, other inhibitors and/or their synergistic effects proved to be responsible for a lower fermentability...
November 11, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29145120/enhanced-nitrogen-removal-from-piggery-wastewater-with-high-nh4-and-low-cod-tn-ratio-in-a-novel-upflow-microaerobic-biofilm-reactor
#7
Jia Meng, Jiuling Li, Jianzheng Li, Philip Antwi, Kaiwen Deng, Jun Nan, Pianpian Xu
To enhance nutrient removal more cost-efficiently in microaerobic process treating piggery wastewater characterized by high ammonium (NH4(+)-N) and low chemical oxygen demand (COD) to total nitrogen (TN) ratio, a novel upflow microaerobic biofilm reactor (UMBR) was constructed and the efficiency in nutrient removal was evaluated with various influent COD/TN ratios and reflux ratios. The results showed that the biofilm on the carriers had increased the biomass in the UMBR and enhanced the enrichment of slow-growth-rate bacteria such as nitrifiers, denitrifiers and anammox bacteria...
November 11, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29156369/mixed-bacterial-culture-mediated-direct-conversion-of-bio-glycerol-to-diols
#8
Roopa Parate, Rasika Mane, Mahesh Dharne, Chandrashekhar Rode
Direct and economic transformation of biodiesel derived crude glycerol is gaining more significance. During screening of bacterial cultures Klebsiella pneumoniae and Enterobacter aerogenes were able to convert crude bio-glycerol to 2,3-butanediol (2,3-BDO) and 1,3-propanediol (1,3-PDO), as major compounds, ethanol and acetoin as minor compounds, with a conversion of 69% and 79% respectively. Process optimization could achieve maximum conversion at pH 7.0, 37 °C, 30-40 g/L glycerol and 1.5 g of inoculum until 120 h...
November 10, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29153646/waste-to-biodiesel-a-preliminary-assessment-for-saudi-arabia
#9
M Rehan, J Gardy, A Demirbas, U Rashid, W M Budzianowski, Deepak Pant, A S Nizami
This study presents a preliminary assessment of biodiesel production from waste sources available in the Kingdom of Saudi Arabia (KSA) for energy generation and solution for waste disposal issues. A case study was developed under three different scenarios: (S1) KSA population only in 2017, (S2) KSA population and pilgrims in 2017, and (S3) KSA population and pilgrims by 2030 using the fat fraction of the municipal solid waste. It was estimated that S1, S2, and S3 scenarios could produce around 1.08, 1.10 and 1...
November 10, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29161573/decentralized-biorefinery-for-lignocellulosic-biomass-integrating-anaerobic-digestion-with-thermochemical-conversion
#10
Chayanon Sawatdeenarunat, Hyungseok Nam, Sushil Adhikari, Shihwu Sung, Samir Kumar Khanal
Anaerobic digestion (AD) of lignocellulosic biomass i.e. Napier grass (Pennisetum purpureum), was investigated via a series of batch and bench-scale experiments. Two semi-continuous bench-scale horizontal bioreactors were operated in parallel for nearly 300 days, and the reactors were able to handle the organic loading rate (OLR) up to 6 kg volatile solids (VS)/m(3)-d, which was among the highest OLR reported in the literature for lignocellulosic biomass. Hemicellulose was the main structural carbohydrate of lignocellulosic biomass per unit respective mass (dry weight) basis contributing to methane production...
November 9, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29153649/demonstration-and-optimization-of-sequential-microaerobic-dark-and-photo-fermentation-biohydrogen-production-by-immobilized-rhodobacter-capsulatus-jp91
#11
Emrah Sağır, Meral Yucel, Patrick C Hallenbeck
Hydrogen generation from complex substrates composed of simple sugars has the potential to mitigate future worldwide energy demand. The biohydrogen potential of a sequential microaerobic dark- and photo-fermentative system was investigated using immobilized Rhodobacter capsulatus JP91. Biological hydrogen production from glucose was carried out using a batch process and a bench-scale bioreactor. Response surface methodology with a Box-Behnken design was employed to optimize key parameters such as inoculum concentration, oxygen concentration, and glucose concentration...
November 9, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29136942/targeted-poly-3-hydroxybutyrate-co-3-hydroxyvalerate-bioplastic-production-from-carbon-dioxide
#12
Stef Ghysels, Md Salatul Islam Mozumder, Heleen De Wever, Eveline I P Volcke, Linsey Garcia-Gonzalez
A microbial production process was developed to convert CO2 and valeric acid into tailored poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) bioplastics. The aim was to understand microbial PHBV production in mixotrophic conditions and to control the monomer distribution in the polymer. Continuous sparging of CO2 with pulse and pH-stat feeding of valeric acid were evaluated to produce PHBV copolyesters with predefined properties. The desired random monomer distribution was obtained by limiting the valeric acid concentration (below 1 gL(-1))...
November 9, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29153651/enhanced-l-ornithine-production-by-systematic-manipulation-of-l-ornithine-metabolism-in-engineered-corynebacterium-glutamicum-s9114
#13
Bin Zhang, Li-Quan Ren, Miao Yu, Ying Zhou, Bang-Ce Ye
l-Ornithine is a non-protein amino acid with extensive applications in medicine and the food industry. Currently, l-ornithine is produced by microbial fermentation; however, this process needs to be further improved in terms of l-ornithine productivity and cost reduction. In this study, overexpression of LysE was observed to increase l-ornithine production in engineered Corynebacterium glutamicum S9114. To overcome the drawbacks of using a plasmid to express LysE, Ptac, a strong promoter, was inserted in the upstream region of lysE on the chromosome...
November 8, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29153644/propionic-acid-production-from-soy-molasses-by-propionibacterium-acidipropionici-fermentation-kinetics-and-economic-analysis
#14
Hopen Yang, Zhongqiang Wang, Meng Lin, Shang-Tian Yang
Propionic acid (PA) is a specialty chemical; its calcium salt is widely used as food preservative. Soy molasses (SM), a low-value byproduct from soybean refinery, contains sucrose and raffinose-family oligosaccharides (RFO), which are difficult to digest for most animals and industrial microorganisms. The feasibility of using SM for PA production by P. acidipropionici, which has genes encoding enzymes necessary for RFO hydrolysis, was studied. With corn steep liquor as the nitrogen source, stable long-term PA production from SM was demonstrated in sequential batch fermentations, achieving PA productivity of >0...
November 8, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29153653/ferroferric-oxide-triggered-possible-direct-interspecies-electron-transfer-between-syntrophomonas-and-methanosaeta-to-enhance-waste-activated-sludge-anaerobic-digestion
#15
Zisheng Zhao, Yang Li, Qilin Yu, Yaobin Zhang
ZVI was reported to enrich H2-utilizing methanogens that enhanced interspecies H2 transfer, while Fe(III) oxide served as a conductive material to promote direct interspecies electron transfer (DIET). However, the interaction of these two modes in anaerobic digestion has not been clarified yet. In this study, when adding Fe3O4 and ZVI simultaneously into an anaerobic digester, the abundance of hydrogenotrophic methanogens decreased drastically compared to ZVI-added digester and Fe-free digester. However, the methane production of ZVI + Fe3O4 added digester were 68...
November 6, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29153645/protein-corona-between-nanoparticles-and-bacterial-proteins-in-activated-sludge-characterization-and-effect-on-nanoparticle-aggregation
#16
Peng Zhang, Xiao-Yan Xu, You-Peng Chen, Meng-Qian Xiao, Bo Feng, Kai-Xun Tian, Yue-Hui Chen, You-Zhi Dai
In this work, the protein coronas of activated sludge proteins on TiO2 nanoparticles (TNPs) and ZnO nanoparticles (ZNPs) were characterized. The proteins with high affinity to TNPs and ZNPs were identified by shotgun proteomics, and their effects of on the distributions of TNPs and ZNPs in activated sludge were concluded. In addition, the effects of protein coronas on the aggregations of TNPs and ZNPs were evaluated. Thirty and nine proteins with high affinities to TNPs and ZNPs were identified, respectively...
November 6, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29145126/pyrolysis-gasification-of-pine-sawdust-biomass-briquettes-under-carbon-dioxide-atmosphere-study-on-carbon-dioxide-reduction-utilization-and-biochar-briquettes-physicochemical-properties
#17
Zewei Liu, Fengxia Zhang, Huili Liu, Fei Ba, Sijia Yan, Jianhang Hu
In this paper, the effects of temperature and CO2 content on CO2 reduction (utilization) efficiency and physicochemical properties of pyrolysis/gasification biochar briquettes were investigated. The CO2 reduction (utilization) efficiency (CRE) reached the peak value of 74.9% in a 10% CO2/90% N2 atmosphere at 600 °C. The crackings of hydroxyl, CC bonds, methyl and methylene groups of biochars briquette were enhanced by high temperature and CO2. The increase of CO2 concentration was beneficial for developing biochar microporosity, but not conductive to enhance higher heating value, volume density and durability of biochar briquette...
November 6, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29145118/long-term-effects-of-combined-divalent-copper-and-tetracycline-on-the-performance-microbial-activity-and-community-in-a-sequencing-batch-reactor
#18
Zichao Wang, Pinghui Xia, Mengchun Gao, Kedong Ma, Zhiwei Deng, Junfeng Wei, Jing Zhang, Lin Wang, Guoxia Zheng, Yusuo Yang, Jinbo Chen, Yuejing Wang
The long-term effects of combined divalent copper (Cu(II)) and tetracycline (TC) on the performance, microbial activity and community in a sequencing batch reactor (SBR) were investigated. The addition of Cu(II), TC or mixed Cu(II)/TC caused the decrease of the organics and nitrogen removal efficiencies, and their decreased degrees were the lowest at the addition of mixed Cu(II)/TC. The increase of mixed Cu(II)/TC concentrations in the influent did not change the antagonistic effects between Cu(II) and TC on nitrifying and denitrifying activities...
November 6, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29145114/boosting-nannochloropsis-oculata-growth-and-lipid-accumulation-in-a-lab-scale-open-raceway-pond-characterized-by-improved-light-distributions-employing-built-in-planar-waveguide-modules
#19
Yahui Sun, Yun Huang, Qiang Liao, Ao Xia, Qian Fu, Xun Zhu, Jingwei Fu
Aiming at alleviating the adverse effect of poor light penetrability on microalgae growth, planar waveguide modules functioned as diluting and redistributing the intense incident light within microalgae culture more homogeneously were introduced into a lab-scale open raceway pond (ORP) for Nannochloropsis oculata cultivation. As compared to the conventional ORP, the illumination surface area to volume ratio and effective illuminated volume percentage in the proposed ORP were respectively improved by 5.53 times and 19...
November 6, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29145113/denitrification-behavior-and-microbial-community-spatial-distribution-inside-woodchip-based-solid-phase-denitrification-w-spd-bioreactor-for-nitrate-contaminated-water-treatment
#20
Jiamin Zhao, Chuanping Feng, Shuang Tong, Nan Chen, Shanshan Dong, Tong Peng, Shunlong Jin
The NO3(-) removal pathway and microorganisms change along with the height of an up-flow W-SPD bioreactor was investigated in this study. Modeling and microbial community analysis were used to analyze the denitrification behavior in W-SPD bioreactor. The results showed that NO3(-) removal rate matched for zero-order (R(2) > 0.97) and first-order (R(2) > 0.94) combination Michaelis-Menten kinetics, whereas microbial reaction rate suited for modified logistic model (R(2) > 0.99). The excellent denitrification performance (92...
November 6, 2017: Bioresource Technology
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