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Bioresource Technology

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https://www.readbyqxmd.com/read/28538202/development-of-nanobiocatalysts-through-the-immobilization-of-pseudomonas-fluorescens-lipase-for-applications-in-efficient-kinetic-resolution-of-racemic-compounds
#1
Bharat P Dwivedee, Jayeeta Bhaumik, Shushil K Rai, Joydev K Laha, Uttam C Banerjee
The present work reports covalent immobilization of Pseudomonas fluorescens lipase (PFL) on functionalized multiwalled carbon nanotubes (MWCNTs) as a nanobiocatalyst (NBC). This nanobiocatalyst facilitates efficient kinetic resolution of (RS)-1-phenylethanol into (S)-1-phenylethanol [C=49.7%, eep=99.5%, ees=98.1% and E value=191.4]. The immobilized preparation (MWCNTs-PFL) showed ten-fold increase in activity, thermal stability upto 80 °C and recyclability (8 cycles). MWCNTs-PFL nanobioconjugate demonstrated better stability and enhanced activity compared to covalently immobilized PFL on other matrices (silver nanoparticles, gold nanoparticles and chitosan beads) used for the study...
May 11, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28531852/two-step-sequential-pretreatment-for-the-enhanced-enzymatic-hydrolysis-of-coffee-spent-waste
#2
Rajeev Ravindran, Swarna Jaiswal, Nissreen Abu-Ghannam, Amit K Jaiswal
In the present study, eight different pretreatments of varying nature (physical, chemical and physico-chemical) followed by a sequential, combinatorial pretreatment strategy was applied to spent coffee waste to attain maximum sugar yield. Pretreated samples were analysed for total reducing sugar, individual sugars and generation of inhibitory compounds such as furfural and hydroxymethyl furfural (HMF) which can hinder microbial growth and enzyme activity. Native spent coffee waste was high in hemicellulose content...
May 11, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28539241/improving-of-lipid-productivity-of-the-oleaginous-microalgae-chlorella-pyrenoidosa-via-atmospheric-and-room-temperature-plasma-artp
#3
Song Cao, Xu Zhou, Wenbiao Jin, Feng Wang, Renjie Tu, Songfang Han, Hongyi Chen, Chuan Chen, Guo-Jun Xie, Fang Ma
In this study, an efficient screening program was established with ARTP. Five strains from oleaginous microalgae Chlorella pyrenoidosa were screened from mutant library after mutagenizing by ARTP. Among them, the optimal mutant strain was named as II-H6. In the BG11 medium, the OD680 of II-H6 in stationary phase were increased by 32.08% than the original strain. Meanwhile, compared with the original strain, the dry weight and lipid productivity of II-H6 were increased by 22.07% and 16.85%, respectively. II-H6 showed a good genetic stability in BG11 medium and the optimum growth temperature and pH were 33°C and 9...
May 10, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28533063/photosynthetic-bacteria-based-membrane-bioreactor-as-post-treatment-of-an-anaerobic-membrane-bioreactor-effluent
#4
E González, O Díaz, I Ruigómez, C R de Vera, L E Rodríguez-Gómez, J Rodríguez-Sevilla, L Vera
Anaerobic membrane bioreactors have attracted increasing interest in the field of wastewater treatment. However, significant amounts of organic matter, nitrogen and sulphide in the effluent may limit its reuse. A photosynthetic bacteria-based membrane bioreactor is proposed for the further treatment of this effluent. A pilot-scale unit was run outdoor for over 900h to assess the process performance at short hydraulic retention time. After an initial biomass development, simultaneous removal of soluble organic matter and nitrogen was achieved (65% and 39%, respectively)...
May 10, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28531859/the-role-of-conductive-materials-in-the-start-up-period-of-thermophilic-anaerobic-system
#5
Wangwang Yan, Nan Shen, Yeyuan Xiao, Yun Chen, Faqian Sun, Vinay Kumar Tyagi, Yan Zhou
The major obstacle for thermophilic anaerobic digestion (TAD) is the inhibited microorganism activity and process instability during the start-up period. This study proposed a strategy to accelerate and stabilize the thermophilic reactors start-up via adding conductive materials. The results show that methane production rate in conductive materials supplemented (CMS) reactors was almost two times higher than the control reactors. Caloramator sp., a candidate of electroactive bacteria, was significantly enriched in the carbon nano-tube (CNT) supplemented groups (12...
May 10, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28531856/low-cost-digester-monitoring-under-realistic-conditions-rural-use-of-biogas-and-digestate-quality
#6
L Castro, H Escalante, J Jaimes-Estévez, L J Díaz, K Vecino, G Rojas, L Mantilla
The purpose of this work was to assess the behaviour of anaerobic digestion of cattle manure in a rural digester under realistic conditions, and estimate the quality and properties of the digestate. The data obtained during monitoring indicated that the digester operation was stable without risk of inhibition. It produced an average of 0.85Nm(3)biogas/d at 65.6% methane, providing an energy savings of 76%. In addition, the digestate contained high nutrient concentrations, which is an important feature of fertilizers...
May 10, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28531854/achieve-efficient-nitrogen-removal-from-real-sewage-in-a-plug-flow-integrated-fixed-film-activated-sludge-ifas-reactor-via-partial-nitritation-anammox-pathway
#7
Yandong Yang, Liang Zhang, Jun Cheng, Shujun Zhang, Baikun Li, Yongzhen Peng
This study tested the feasibility of plug-flow integrated fixed-film activated sludge (IFAS) reactor in applying sewage partial nitritation/anammox (PN/A) process. The IFAS reactor was fed with real pre-treated sewage (C/N ratio=1.3) and operated for 200days. High nitrogen removal efficiency of 82% was achieved with nitrogen removal rates of 0.097±0.019kgN/(m(3)·d). Therefore, plug-flow IFAS reactor could be an alternative to applying sewage PN/A process. Besides, it was found that the stability of sewage PN/A process was significantly affected by residual ammonium...
May 10, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28531853/methane-production-in-an-anaerobic-osmotic-membrane-bioreactor-using-forward-osmosis-effect-of-reverse-salt-flux
#8
Sheng Li, Youngjin Kim, Sherub Phuntsho, Laura Chekli, Ho Kyong Shon, TorOve Leiknes, Noreddine Ghaffour
This study investigated the impact of reverse salt flux (RSF) on microbe community and bio-methane production in a simulated fertilizer driven FO-AnMBR system using KCl, KNO3 and KH2PO4 as draw solutes. Results showed that KH2PO4 exhibited the lowest RSF in terms of molar concentration 19.1mM/(m(2).h), while for KCl and KNO3 it was 32.2 and 120.8mM/(m(2).h), respectively. Interestingly, bio-methane production displayed an opposite order with KH2PO4, followed by KCl and KNO3. Pyrosequencing results revealed the presence of different bacterial communities among the tested fertilizers...
May 10, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28531851/comparative-life-cycle-assessment-study-on-environmental-impact-of-oil-production-from-micro-algae-and-terrestrial-oilseed-crops
#9
S Jez, D Spinelli, A Fierro, A Dibenedetto, M Aresta, E Busi, R Basosi
In this study the LCA methodology is applied in order to satisfy two goals: i) to evaluate the hot spots in site-specific production chain of biodiesel from terrestrial and micro-algae feedstock; ii) to compare quantitatively, utilizing primary data, the impacts of the first generation in respect to the third generation bio-fuels. Results show that micro-algae are neither competitive yet with traditional oil crops nor with fossil fuel. The use of renewable technologies as photovoltaics and biogas self production might increase the competitiveness of micro-algae oil...
May 10, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28531849/heterotrophy-of-filamentous-oleaginous-microalgae-tribonema-minus-for-potential-production-of-lipid-and-palmitoleic-acid
#10
Wenjun Zhou, Hui Wang, Lin Chen, Wentao Cheng, Tianzhong Liu
Heterotrophic fermentation and high valuable co-product producing are thought to be effective ways to improve the economic viability and feasibility of commercial production of microalgae biofuels. This work reported the heterotrophic cultivation of Tribonema minus for lipid and palmitoleic acid (a novel functional fatty acid) production. Firstly, the heterotrophic ability of T. minus was identified for the first time with significant promotion in biomass and lipid productivity, and glucose and urea were then selected as the optimal carbon and nitrogen sources...
May 10, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28526282/an-oleaginous-filamentous-microalgae-tribonema-minus-exhibits-high-removing-potential-of-industrial-phenol-contaminants
#11
Tianyou Cheng, Wei Zhang, Wenlei Zhang, Guanhua Yuan, Hui Wang, Tianzhong Liu
Discharge of industrial phenol contaminants could cause great harm on natural environment. Through oleaginous microalgae cultivation in phenolic wastewater, pollutants can be phototrophically biofixed into biomass as feedstock for bioenergy production. It was firstly reported in this study that, an oleaginous filamentous microalgae Tribonema minus exhibited strong environmental phenol removal ability. T. minus filaments showed 449.46mgg(-1) of phenol-uptake capacity, obviously higher than those strains with low phenol absorption such as Scenedesmus dimorphus...
May 10, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28522198/efficient-production-of-2-3-butanediol-by-recombinant-saccharomyces-cerevisiae-through-modulation-of-gene-expression-by-cocktail-%C3%AE-integration
#12
Ryosuke Yamada, Kazuki Wakita, Ryosuke Mitsui, Riru Nishikawa, Hiroyasu Ogino
In this study, the expression of 4 genes encoding α-acetolactate synthase, α-acetolactate decarboxylase, 2,3-butanediol dehydrogenase, and NADH oxidase was modulated using a previously developed cocktail δ-integration strategy. The resultant strain, YPH499/dPdAdG/BD6-10, was used in a fed-batch cultivation for the production of 2,3-butanediol. The concentration, production rate, and yield obtained were 80.0g/L, 4.00g/L/h, and 41.7%, respectively. The production rate and yield of the compound obtained are higher for this strain compared to reports published for Saccharomyces cerevisiae so far...
May 10, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28521227/start-up-and-operation-of-an-aerobic-granular-sludge-system-under-low-working-temperature-inoculated-with-cold-adapted-activated-sludge-from-finland
#13
Alejandro Gonzalez-Martinez, Barbara Muñoz-Palazon, Alejandro Rodriguez-Sanchez, Paula Maza-Márquez, Anna Mikola, Jesus Gonzalez-Lopez, Riku Vahala
An aerobic granular sludge system has been started-up and operated at 7°C temperature using cold-adapted activated sludge as inoculum. The system could form granular biomass due to batch operation allowing for just 5-3min of biomass sedimentation. Scanning electron microscopy showed that fungi helped in the granular biomass formation in the early stages of the granule formation. The removal performance of the system was of 92-95% in BOD5, 75-80% in COD, 70-76% in total nitrogen and 50-60% in total phosphorous...
May 10, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28521225/recovery-of-gold-from-industrial-wastewater-by-extracellular-proteins-obtained-from-a-thermophilic-bacterium-tepidimonas-fonticaldi-at-a2
#14
Yin-Lung Han, Jen-Hao Wu, Chieh-Lun Cheng, Dillirani Nagarajan, Ching-Ray Lee, Yi-Heng Li, Yung-Chung Lo, Jo-Shu Chang
Biosorption has emerged as a promising alternative approach for treating wastewater with dilute metal contents in a green and cost effective way. In this study, extracellular proteins of an isolated thermophilic bacterium (Tepidimonas fonticaldi AT-A2) were used as biosorbent to recover precious metal (i.e., Au) from wastewater. The Au (III) adsorption capacity on the T. fonticaldi AT-A2 proteins was the highest when the pH was set at about 4.0-5.0. The adsorption capacity increased with increasing temperature from 15 to 70°C...
May 10, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28521224/investigation-of-aqueous-phase-recycling-for-improving-bio-crude-oil-yield-in-hydrothermal-liquefaction-of-algae
#15
Yulin Hu, Shanghuan Feng, Zhongshun Yuan, Chunbao Charles Xu, Amarjeet Bassi
In this study, the aqueous phase obtained from catalytic/non-catalytic hydrothermal liquefaction (HTL) of Chlorella vulgaris was recycled as the reaction medium with an aim to reduce water consumption and increase bio-crude oil yield. Although both Na2CO3 and HCOOH catalysts have been proven to be effective for promoting biomass conversion, the bio-crude oil yield obtained from HTL with Na2CO3 (11.5wt%) was lower than that obtained from the non-catalytic HTL in pure water at 275°C for 50min. While, the HCOOH led to almost the same bio-crude yield from HTL (29...
May 10, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28535493/nutrient-and-heavy-metal-accumulation-in-municipal-organic-waste-from-separate-collection-during-anaerobic-digestion-in-a-two-stage-laboratory-biogas-plant
#16
Christine Knoop, Christina Dornack, Thomas Raab
Municipal organic waste (MOW) is a promising feedstock for biogas plants and separate collection will increase available quantities. To close nutrient circles digestates shall be redistributed to arable land. However, less is known about digestate properties and how they are influenced during digestion. Therefore, changes in nutrient and heavy metal concentration in the solid digestate were investigated during anaerobic treatment of MOW in a two-stage laboratory biogas plant. Results show that the solid digestate is exposed to element accumulation, except for N, P and Mg...
May 8, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28533064/strategies-for-design-of-improved-biocatalysts-for-industrial-applications
#17
REVIEW
Aravind Madhavan, Raveendran Sindhu, Parameswaran Binod, Rajeev K Sukumaran, Ashok Pandey
Biocatalysts are creating increased interest among researchers due to their unique properties. Several enzymes are efficiently produced by microorganisms. However, the use of natural enzymes as biocatalysts is hindered by low catalytic efficiency and stability during various industrial processes. Many advanced enzyme technologies have been developed to reshape the existing natural enzymes to reduce these limitations and prospecting of novel enzymes. Frequently used enzyme technologies include protein engineering by directed evolution, immobilisation techniques, metagenomics etc...
May 8, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28531850/in-situ-injection-of-potassium-hydroxide-into-briquetted-wheat-straw-and-meadow-grass-effect-on-biomethane-production
#18
Lu Feng, Veronica Moset, Wanwu Li, Chang Chen, Henrik Bjarne Møller
Alkaline pretreatment of lignocellulosic biomass has been intensively investigated but heavy water usage and environmental pollution from wastewater limits its industrial application. This study presents a pretreatment technique by in-situ injection of potassium hydroxide concentrations ranging from 0.8% to 10% (w/w) into the briquetting process of wheat straw and meadow grass. Results show that the biomethane yield and hydrolysis rate was improved significantly with a higher impact on wheat straw compared to meadow grass...
May 8, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28522201/adsorption-of-p-nitrophenols-pnp-on-microalgal-biochar-analysis-of-high-adsorption-capacity-and-mechanism
#19
Heshan Zheng, Wanqian Guo, Shuo Li, Yidi Chen, Qinglian Wu, Xiaochi Feng, Renli Yin, Shih-Hsin Ho, Nanqi Ren, Jo-Shu Chang
Biochars derived from three microalgal strains (namely, Chlorella sp. Cha-01, Chlamydomonas sp. Tai-03 and Coelastrum sp. Pte-15) were evaluated for their capacity to adsorb p-nitrophenols (PNP) using raw microalgal biomass and powdered activated carbon (PAC) as the control. The results show that BC-Cha-01 (biochar from Chlorella sp. Cha-01) exhibited a high PNP adsorption capacity of 204.8mgg(-1), which is 250% and 140% higher than that of its raw biomass and PAC, respectively. The adsorption kinetics and equilibrium are well described with pseudo-second-order equation and Freundlich model, respectively...
May 8, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28521231/development-of-an-x-shape-airlift-photobioreactor-for-increasing-algal-biomass-and-biodiesel-production
#20
Hoang-Minh Pham, Ho Seok Kwak, Min-Eui Hong, Jeewon Lee, Won Seok Chang, Sang Jun Sim
The aim of this work was to develop a high efficient photobioreactor for increasing biomass and lipid production in microalgae by assessment of the hydrodynamic properties and kLa which are important parameters for improving the algal cultivation efficiency. We designed three different photobioreactors (H-Shape, X-Shape and serial-column). Among them, X-Shape showed the highest hydrodynamic properties and kLa for algal cultivation. Thus, we evaluated the biomass and the lipid production in a 20L scale-up X-Shape photobioreactor...
May 8, 2017: Bioresource Technology
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