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Journal of Nematology

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https://www.readbyqxmd.com/read/27765995/discopersicus-n-gen-a-new-member-of-the-family-tylenchidae-%C3%A3-rley-1880-with-detailed-sem-study-on-two-known-species-of-the-genus-discotylenchus-siddiqi-1980-nematoda-tylenchidae-from-iran
#1
Ali Yaghoubi, Ebrahim Pourjam, Sergio Álvarez-Ortega, Gracia Liébanas, Mohammad Reza Atighi, Majid Pedram
Discopersicus iranicus n. gen., n. comb., previously described from Iran as a new species under the genus Discotylenchus, is illustrated using light microscope and scanning electron microscope (SEM) observations and further studied using molecular characters. SEM studies revealed the newly proposed genus has oblique amphidial apertures on the lateral sides of the lip region. SEM images are also provided for two species of Discotylenchus, namely D. discretus and D. brevicaudatus, as the first SEM study of the genus...
September 2016: Journal of Nematology
https://www.readbyqxmd.com/read/27765994/morphological-and-molecular-characterization-of-gracilacus-wuae-n-sp-nematoda-criconematoidea-associated-with-cow-parsnip-heracleum-maximum-in-ontario-canada
#2
Qing Yu, Weimin Ye, Tom Powers
Gracilacus wuae n. sp. from soil associated with cow parsnip in Ontario, Canada is described and illustrated. Morphologically, females have a long stylet ranging from 80 to 93 µm long, the lip region not offset from the body contour, without lateral lips but with large and flat submedian lobes, the mouth opening slit-like elongated laterally and surrounded by lateral flaps, the excretory pore is anterior to the knobs of the stylet; males without stylet and the pharynx degenerated. The fourth-stage juveniles lack a stylet, the pharynx degenerated, and can be differentiated into preadult females and males based on the position of the genital primordia...
September 2016: Journal of Nematology
https://www.readbyqxmd.com/read/27765993/mitochondrial-haplotype-based-identification-of-root-knot-nematodes-meloidogyne-spp-on-cut-foliage-crops-in-florida
#3
Richard Baidoo, Soumi Joseph, Tesfamariam M Mengistu, Janete A Brito, Robert McSorley, Robert H Stamps, William T Crow
Florida accounts for more than 75% of the national cut foliage production. Unfortunately, root-knot nematodes (RKN) (Meloidogyne spp.) are a serious problem on these crops, rendering many farms unproductive. Currently, information on the Meloidogyne spp. occurring on most commonly cultivated cut foliage crops in Florida, and tools for their rapid identification are lacking. The objectives of this study were to (i) identify specific RKN infecting common ornamental cut foliage crops in Florida and (ii) evaluate the feasibility of using the mtDNA haplotype as a molecular diagnostic tool for rapid identification of large samples of RKN...
September 2016: Journal of Nematology
https://www.readbyqxmd.com/read/27765992/evaluation-of-steam-and-soil-solarization-for-meloidogyne-arenaria-control-in-florida-floriculture-crops
#4
Nancy Kokalis-Burelle, Erin N Rosskopf, David M Butler, Steven A Fennimore, John Holzinger
Steam and soil solarization were investigated for control of the root-knot nematode Meloidogyne arenaria in 2 yr of field trials on a commercial flower farm in Florida. The objective was to determine if preplant steam treatments in combination with solarization, or solarization alone effectively controlled nematodes compared to methyl bromide (MeBr). Trials were conducted in a field with naturally occurring populations of M. arenaria. Treatments were solarization alone, steam treatment after solarization using standard 7...
September 2016: Journal of Nematology
https://www.readbyqxmd.com/read/27765991/effect-of-application-timing-of-oxamyl-in-nonbearing-raspberry-for-pratylenchus-penetrans-management
#5
Inga A Zasada, Thomas W Walters
In 2012, the Washington raspberry (Rubus idaeus) industry received a special local needs (SLN) 24(c) label to apply Vydate L(®) (active ingredient oxamyl) to nonbearing raspberry for the management of Pratylenchus penetrans. This is a new use pattern of this nematicide for raspberry growers; therefore, research was conducted to identify the optimum spring application timing of oxamyl for the suppression of P. penetrans. Three on-farm trials in each of 2012 and 2013 were established in Washington in newly planted raspberry trials on a range of varieties...
September 2016: Journal of Nematology
https://www.readbyqxmd.com/read/27765990/curative-control-of-the-peachtree-borer-using-entomopathogenic-nematodes
#6
David I Shapiro-Ilan, Ted E Cottrell, Russell F Mizell, Dan L Horton
The peachtree borer, Synanthedon exitiosa (Say 1823), is a major pest of stone fruit trees in North America. Current management relies upon preventative control using broad-spectrum chemical insecticides, primarily chlorpyrifos, applied in the late summer or early fall. However, due to missed applications, poor application timing, or other factors, high levels of S. exitiosa infestation may still occur and persist through the following spring. Curative treatments applied in the spring to established infestations would limit damage to the tree and prevent the next generation of S...
September 2016: Journal of Nematology
https://www.readbyqxmd.com/read/27765989/coronostoma-claireae-n-sp-nematoda-rhabditida-oxyuridomorpha-coronostomatidae-from-the-indigenous-milliped-narceus-gordanus-chamberlain-1943-diplopoda-spirobolida-in-ocala-national-forest-florida
#7
Gary Phillips, Ernest C Bernard, Robert J Pivar, John K Moulton, Rowland M Shelley
Twenty-four individuals of Narceus gordanus (Diplopoda: Spirobolidae) were collected in Ocala National Forest, FL, between November 2013 and July 2014. Each specimen was dissected to extract the intestine, which was removed and examined for parasitic nematodes. Coronostoma claireae n. sp. was collected from the hindgut and midgut of 10 specimens, and its morphology was examined with brightfield, differential interference contrast, phase contrast, and scanning electron microscopy. This species is separated from other Coronostoma spp...
September 2016: Journal of Nematology
https://www.readbyqxmd.com/read/27765988/steinernema-biddulphi-n-sp-a-new-entomopathogenic-nematode-nematoda-steinernematidae-from-south-africa
#8
Harun Cimen, Vladimír Půža, JiŘí NermuŤ, Justin Hatting, Tshima Ramakuwela, Selcuk Hazir
A new species of entomopathogenic nematode (EPN), Steinernema biddulphi n. sp., was isolated from a maize field in Senekal, Free State Province of South Africa. Morphological and molecular studies indicated the distinctness of S. biddulphi n. sp. from other Steinernema species. Steinernema biddulphi n. sp. is characterized IJs with average body length of 663 μm (606-778 μm), lateral fields with six ridges in mid-body region forming the formula 2,6,2. Excretory pore located anterior to mid-pharynx (D% = 46)...
September 2016: Journal of Nematology
https://www.readbyqxmd.com/read/27765987/first-report-of-heterorhabditis-amazonensis-from-venezuela-and-characterization-of-three-populations
#9
Naiyulin Morales, Patricia Morales-Montero, Vladimir Puza, Ernesto San-Blas
During a survey in western Venezuela in 2011, three new populations of Heterorhabditis amazonensis (LPV081, LPV156, and LPV498) were isolated. Some differences were found in terms of morphometry compared with the original description; however, the distance from the anterior end to the excretory pore is the most variable character; significantly shorter in all infective juveniles and in other developmental stages depending on the population. According to a Principal Component Analysis, LPV498 possesses more differences in morphometric characteristics and can be separated from the other two...
September 2016: Journal of Nematology
https://www.readbyqxmd.com/read/27765986/first-report-of-the-spiral-nematode-rotylenchus-incultus-nematoda-hoplolaimidae-from-cultivated-olive-in-tunisia-with-additional-molecular-data-on-rotylenchus-eximius
#10
Ilhem Guesmi-Mzoughi, Antonio Archidona-Yuste, Carolina Cantalapiedra-Navarrete, Hajer Regaieg, Najet Horrigue-Raouani, Juan E Palomares-Rius, Pablo Castillo
Spiral nematode species of the genus Rotylenchus have been reported on olive (Olea europaea L.) in several Mediterranean countries (Castillo et al., 2010; Ali et al., 2014). Nematological surveys for plant-parasitic nematodes on olive trees were carried out in Tunisia between 2013 and 2014, and two nematode species of Rotylenchus were collected from the rhizosphere of olive cv. Chemlali in several localities of Tunisia (Tables 1,2 [Table: see text] [Table: see text] ). Twenty-two soil samples of 3 to 4 kg were collected with a shovel from the upper 50 cm of soil from arbitrarily chosen olive trees...
September 2016: Journal of Nematology
https://www.readbyqxmd.com/read/27765985/first-report-of-longidorus-kuiperi-and-rotylenchus-eximius-from-coastal-sand-dunes-in-crete-greece
#11
Emmanuel A Tzortzakakis, Carolina Cantalapiedra-Navarrete, Antonio Archidona-Yuste, Juan E Palomares-Rius, Pablo Castillo
Plant-parasitic nematode species have been reported on several occasions from coastal sand dunes, including Longidorus and Rotylenchus species (Vovlas et al., 2008; De Luca et al., 2009; Mateille et al., 2014). In April 2016, 10 soil samples of 3 to 4 kg from the rhizosphere of Tamarix smyrnensis with different vegetation around (viz. Elymus farctus, Lycium schweinfurthii, Crithmum maritimum, and Arthrocnemum sp.) were collected for diagnosis of plant-parasitic nematodes. The area of sampling was a coastal sand dune near the archeological site of Komos, southwest of Crete, Greece...
September 2016: Journal of Nematology
https://www.readbyqxmd.com/read/27418706/the-effects-of-nutrient-concentration-addition-of-thickeners-and-agitation-speed-on-liquid-fermentation-of-steinernema-feltiae
#12
Luis G Leite, David I Shapiro-Ilan, Selcuk Hazir, Mark A Jackson
Entomopathogenic nematode production in liquid fermentation still requires improvements to maximize efficiency, yield, and nematode quality. Therefore, this study was aimed at developing a more suitable liquid medium for mass production of Steinernema feltiae, by assessing the effects of nutrient concentration, thickeners (primarily agar), and agitation speed on infective juvenile (IJ) yield. Base medium (BM) contained yeast extract (2.3%), egg yolk (1.25%), NaCl (0.5%), and corn oil (4%). All media were inoculated with Xenorhabdus bovienii, and 2 d later, with 2-d-old S...
June 2016: Journal of Nematology
https://www.readbyqxmd.com/read/27418705/nematode-fauna-of-tropical-rainforest-in-brazil-a-descriptive-and-seasonal-approach
#13
Mercia S O Cardoso, Elvira M R Pedrosa, Howard Ferris, Mario M Rolim, Lamartine S C Oliveira
Studies of nematode assemblages in natural ecosystems can contribute to better understanding of the occurrence, relevance, and ecology of plant-parasitic and other soil nematodes. Nematode assemblages and environmental parameters (organic matter, water content (WC), bulk density (BD), total porosity (Po), soil respiration, and soil texture) were investigated in two seasons (rainy and dry) in two forest areas of the Zona da Mata, Pernambuco State. The aim of our research was to evaluate the heterogeneity between two locations and seasons in the Brazilian Atlantic Forest...
June 2016: Journal of Nematology
https://www.readbyqxmd.com/read/27418704/paurodontella-parapitica-n-sp-nematoda-hexatylina-sphaerularioidea-from-kermanshah-province-western-iran
#14
Mehrab Esmaeili, Ramin Heydari, Weimin Ye
Paurodontella parapitica n. sp., collected from the rhizosphere of an apple tree in Kermanshah province, western Iran, is described. The new species is characterized by a body length of 505 to 723 µm (females) and 480 to 600 µm (males), lip region continuous by depression; 7 to 8 μm broad, 3 to 4 µm high, stylet length 7 to 9 µm or 1 to 1.3 times the lip region diameter, short postuterine sac of 4 to 6 μm long, lateral fields with five to six incisures; outer incisures crenated and inner incisures weakly crenated, excretory pore situated 90 to 100 µm from anterior end; functional males common in the population, with spicules 24 to 26 μm long...
June 2016: Journal of Nematology
https://www.readbyqxmd.com/read/27418703/a-third-new-species-of-aporcelinus-dorylaimida-aporcelaimidae-from-vietnam-with-the-first-sem-study-of-a-representative-of-the-genus
#15
Thi Anh Duong Nguyen, Joaquín Abolafia, Michael Bonkowski, Reyes Peña-Santiago
A new species, the third one from Vietnam, of the genus Aporcelinus is described from natural areas. Aporcelinus falcicaudatus sp. n. is characterized by its 1.28 to 1.63 mm long body, lip region offset by weak constriction and 16 to 18 µm broad, odontostyle 18 to 21 µm at its ventral side, neck 354 to 406 µm long, uterus tripartite and 61 to 95 µm long, V = 50 to 55, tail strongly recurved dorsad and conical (23-31 µm, c = 43-58, c' = 0.7-0.9) with finely rounded tip, and male absent. Scanning electron microscope (SEM) study, the first of a representative of the genus, shows a lip region pattern significantly different from that observed in the typical aporcelaimid taxa...
June 2016: Journal of Nematology
https://www.readbyqxmd.com/read/27418702/sectonema-caobangense-sp-n-from-vietnam-nematoda-dorylaimida-aporcelaimidae
#16
Sergio Álvarez-Ortega, Thi Anh Duong Nguyen, Joaquín Abolafia, Michael Bonkowski, Reyes Peña-Santiago
Sectonema caobangense sp. n. from evergreen forest soil in Vietnam is described, including scanning electron micrograph (SEM) observations and D2-D3 LSU rDNA analysis. The new species is characterized by its 3.12 to 5.80 mm long body, lip region offset by deep constriction and 21 to 23 μm broad, mural tooth 13 to 14 μm long at its ventral side, 940 to 1,112 μm long neck, pharyngeal expansion occupying 61% to 69% of total neck length, uterus a long simple tube-like structure 292 to 363 μm long or 2.7 to 2...
June 2016: Journal of Nematology
https://www.readbyqxmd.com/read/27418701/the-effect-of-endophytic-fungi-on-nematode-populations-in-summer-dormant-and-summer-active-tall-fescue
#17
James K Rogers, Nathan R Walker, Carolyn A Young
Summer-active (continental) and summer-dormant (Mediterranean) tall fescue morphotypes are each adapted to different environmental conditions. Endophyte presence provides plant parasitic nematode resistance, but not with all endophyte strains and cultivar combinations. This study sought to compare effects of four nematode genera on continental and Mediterranean cultivars infected with common toxic or novel endophyte strains. A 6-mon greenhouse study was conducted with continental cultivars, Kentucky 31 (common toxic) and Texoma MaxQ II (novel endophyte) and the Mediterranean cultivar Flecha MaxQ (novel endophyte)...
June 2016: Journal of Nematology
https://www.readbyqxmd.com/read/27418700/reproduction-of-meloidogyne-incognita-race-3-on-flue-cured-tobacco-homozygous-for-rk1-and-or-rk2-resistance-genes
#18
Jill R Pollok, Charles S Johnson, J D Eisenback, T David Reed
Most commercial tobacco cultivars possess the Rk1 resistance gene to races 1 and 3 of Meloidogyne incognita and race 1 of Meloidogyne arenaria, which has caused a shift in population prevalence in Virginia tobacco fields toward other species and races. A number of cultivars now also possess the Rk2 gene for root-knot resistance. Experiments were conducted in 2013 to 2014 to examine whether possessing both Rk1 and Rk2 increases resistance to a variant of M. incognita race 3 compared to either gene alone. Greenhouse trials were arranged in a completely randomized design with Coker 371-Gold (C371G; susceptible), NC 95 and SC 72 (Rk1Rk1), T-15-1-1 (Rk2Rk2), and STNCB-2-28 and NOD 8 (Rk1Rk1 and Rk2Rk2)...
June 2016: Journal of Nematology
https://www.readbyqxmd.com/read/27418699/reproduction-and-damage-potential-of-five-geographical-ditylenchus-africanus-populations-on-peanut
#19
Sonia Steenkamp, Dirk De Waele, Alexander McDonald
Ditylenchus africanus affects peanut quality, which leads to downgrading of consignments and economic losses for producers. This nematode is difficult to control and host-plant resistance may be the most effective way to control it. Recently, the peanut breeding line PC254K1 has been identified as resistant to a D. africanus population from Vaalharts and will be included into the peanut breeding program. The objectives of our study were to compare the reproduction potential of D. africanus geographic populations from five different areas in the peanut production area of South Africa and to assess whether PC254K1 is resistant to all five D...
June 2016: Journal of Nematology
https://www.readbyqxmd.com/read/27418698/efficacy-of-various-application-methods-of-fluensulfone-for-managing-root-knot-nematodes-in-vegetables
#20
Kelly A Morris, David B Langston, Richard F Davis, James P Noe, Don W Dickson, Patricia Timper
Fluensulfone is a new nematicide in the flouroalkenyl chemical group. A field experiment was conducted in 2012 and 2013 to evaluate the efficacy of various application methods of fluensulfone for control of Meloidogyne spp. in cucumber (Cucumis sativus). Treatments of fluensulfone (3.0 kg a.i./ha) were applied either as preplant incorporation (PPI) or via different drip irrigation methods: drip without pulse irrigation (Drip NP), pulse irrigation 1 hr after treatment (Drip +1P), and treatment at the same time as pulse irrigation (Drip =P)...
June 2016: Journal of Nematology
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