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Plant Physiology and Biochemistry: PPB

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https://www.readbyqxmd.com/read/28088021/sanguinarine-induced-oxidative-stress-and-apoptosis-like-programmed-cell-death-al-pcd-in-root-meristem-cells-of-allium-cepa
#1
Aneta Żabka, Konrad Winnicki, Justyna Teresa Polit, Janusz Maszewski
A vast number of studies on plant cell systems clearly indicate that various biotic and abiotic stresses give rise to the uncontrolled increase in the level of reactive oxygen species (ROS). Excess concentrations of ROS result in damage to proteins, lipids, carbohydrates, and DNA, which may lead, in consequence, to the apoptotic cell death. The current study investigates the effects of sanguinarine (SAN), a natural alkaloid derived from the roots of Sanguinaria canadensis, on root apical meristem cells of Allium cepa...
January 4, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28086169/functional-characterization-of-a-novel-jasmonate-zim-domain-interactor-ninja-from-upland-cotton-gossypium-hirsutum
#2
Le Wang, Shu-Ming Wu, Yue Zhu, Qiang Fan, Zhen-Nan Zhang, Guang Hu, Qing-Zhong Peng, Jia-He Wu
The jasmonic acid (JA) signalling pathway plays roles in plant development and defence against biotic and abiotic stresses. We isolated a cotton NINJA (novel interactor of JA ZIM-domain) gene, designated GhNINJA, which contains a 1305 bp open read frame. The GhNINJA gene encodes a 434 amino acid peptide. According to quantitative real-time PCR analysis, GhNINJA is preferentially expressed in roots, and its expression level is greatly induced by Verticillium dahliae infection. Through a virus-induced gene silencing technique, we developed GhNINJA-silenced cotton plants, which had significantly decreased expression of the target gene with an average expression of 6% of the control...
January 4, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28088018/antibacterial-serine-protease-from-wrightia-tinctoria-purification-and-characterization
#3
Sakthivel Muthu, Venkatesh Babu Gopal, Selvakumar Soundararajan, Karthikeyan Nattarayan, Karthik S Narayan, Mythileeswari Lakshmikanthan, Sathuvan Malairaj, Palani Perumal
A serine protease was purified from the leaves of Wrightia tinctoria by sequential flow through method comprising screening, optimization, ammonium sulfate precipitation, gel filtration and ion exchange column chromatography. The yield and purification fold obtained were 11.58% and 9.56 respectively. A single band of serine protease was visualized on SDS-PAGE and 2-D gel electrophoretic analyses were revealed with the molecular mass of 38.5 kDa. Serine protease had an optimum pH of 8.0 and was stable at 45°C with high relative protease activity...
January 3, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28064119/identification-and-expression-analysis-of-the-nac-transcription-factor-family-in-durum-wheat-triticum-turgidum-l-ssp-durum
#4
Mohammed Najib Saidi, Dhawya Mergby, Faiçal Brini
The NAC (NAM, ATAF and CUC) proteins belong to one of the largest plant-specific transcription factor (TF) families and play important roles in plant development processes, response to biotic and abiotic cues and hormone signaling. Our analysis led to the identification of 168 NAC genes in durum wheat, including nine putative membrane-bound TFs and 48 homeologous genes pairs. Phylogenetic analyses of TtNACs along with their Arabidopsis, grape, barley and rice counterparts divided these proteins into 8 phylogenetic groups and allowed the identification of TtNAC-A7, TtNAC-B35, TtNAC-A68, TtNAC-B69 and TtNAC-A43 as homologs of OsNAC1, OsNAC8, OsNTL2, OsNTL5 and ANAC025/NTL14, respectively...
January 2, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28049060/a-comparative-study-of-the-dynamic-accumulation-of-polyphenol-components-and-the-changes-in-their-antioxidant-activities-in-diploid-and-tetraploid-lonicera-japonica
#5
Dexin Kong, Yanqun Li, Mei Bai, Yali Deng, Guangxin Liang, Hong Wu
Polyploidization is an effective method to achieve a higher yield of secondary metabolism active ingredients in medicinal plants. Polyphenols are the main active substances that contribute to the antioxidant activity of Lonicera japonica. For studying on the effect of chromosome doubling and harvest time on the dynamic accumulation of the main active substances and antioxidant capabilities of L. japonica, the polyphenol composition contents (7 phenolic acids and 3 flavonoids) and the antioxidant capacity in buds and flowers of diploid and tetraploid L...
December 28, 2016: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28088020/sulphur-alters-chromium-vi-toxicity-in-solanum-melongena-seedlings-role-of-sulphur-assimilation-and-sulphur-containing-antioxidants
#6
Madhulika Singh, Bishwajit Kumar Kushwaha, Samiksha Singh, Vipin Kumar, Vijay Pratap Singh, Sheo Mohan Prasad
The present study investigates modulation in hexavalent chromium [Cr(VI) 25 μM] toxicity by sulphur (S; 0.5, 1.0 and 1.5 mM S as low (LS), medium (MS) and high sulphur (HS), respectively) in Solanum melongena (eggplant) seedlings. Biomass accumulation (fresh and dry weights), photosynthetic pigments, photosynthetic oxygen evolution and S content were declined by Cr(VI) toxicity. Furthermore, fluorescence characteristics (JIP-test) were also affected by Cr(VI), but Cr(VI) toxicity on photosystem II photochemistry was ameliorated by HS treatment via reducing damaging effect on PS II reaction centre and its reduction side...
December 27, 2016: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28068641/oil-palm-drought-inducible-dreb1-induced-expression-of-dre-crt-and-non-dre-crt-containing-genes-in-lowland-transgenic-tomato-under-cold-and-peg-treatments
#7
Azzreena Mohamad Azzeme, Siti Nor Akmar Abdullah, Maheran Abd Aziz, Puteri Edaroyati Megat Wahab
Dehydration-responsive element binding (DREB) transcription factor plays an important role in controlling the expression of abiotic stress responsive genes. An intronless oil palm EgDREB1 was isolated and confirmed to be a nuclear localized protein. Electrophoretic mobility shift and yeast one-hybrid assays validated its ability to interact with DRE/CRT motif. Its close evolutionary relation to the dicot NtDREB2 suggests a universal regulatory role. In order to determine its involvement in abiotic stress response, functional characterization was performed in oil palm seedlings subjected to different levels of drought severity and in EgDREB1 transgenic tomato seedlings treated by abiotic stresses...
December 27, 2016: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28064118/pb-low-doses-induced-genotoxicity-in-lactuca-sativa-plants
#8
S Silva, P Silva, H Oliveira, I Gaivão, M Matos, O Pinto-Carnide, C Santos
Soil and water contamination by lead (Pb) remains a topic of great concern, particularly regarding crop production. The admissible Pb values in irrigation water in several countries range from ≈0.1 to ≈5 mg L(-1). In order to evaluate putative effects of Pb within legal doses on crops growth, we exposed Lactuca sativa seeds and seedlings to increasing doses of Pb(NO3)2 up to 20 mg L(-1). The OECD parameter seed germination and seedling/plant growth were not affected by any of the Pb-concentrations used...
December 27, 2016: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28049059/arsenic-induced-genotoxic-responses-and-their-amelioration-by-diphenylene-iodonium-24-epibrassinolide-and-proline-in-glycine-max-l
#9
Vibhuti Chandrakar, Bhumika Yadu, Rakesh Kumar Meena, Amit Dubey, S Keshavkant
Presence of the toxic metalloid, "arsenic (As)" is ubiquitous in the environment especially in the soil and water. Its excess availability in the soil retards growth and metabolism of plants via (a) slowing down the cell division/elongation, (b) overproduction of reactive oxygen species (ROS), (c) modulation of antioxidant enzymes, and (d) alteration of DNA profile/genomic template stability (GTS). In the current study, diphenylene iodonium (DPI), 24-epibrassinolide (EBL) and proline (Pro) were used to analyze their roles in eliminating the adverse effects of As...
December 26, 2016: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28040634/insights-on-germinability-and-desiccation-tolerance-in-developing-neem-seeds-azadirachta-indica-role-of-aos-antioxidative-enzymes-and-dehydrin-like-protein
#10
Balram Sahu, Alok Kumar Sahu, Srinivasa Rao Chennareddy, Avinash Soni, Subhash Chandra Naithani
The germinability and desiccation tolerance (DT) in developing seed are regulated by cellular metabolism involving active oxygen species (AOS) and protective proteins during maturation drying. The aim of the present investigation was to unravel the functions of AOS (superoxide, H2O2 and OH-radical), antioxidative enzymes (SOD, CAT and APX) and dehydrin-like proteins in regulating the germinability and DT in undried and artificially desiccated developing neem seeds. Germination was first observed in seeds of 8 weeks after anthesis (waa) whereas DT was noticed from 9 waa...
December 25, 2016: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28039816/overexpression-of-a-stress-responsive-u-box-protein-gene-vapub-affects-the-accumulation-of-resistance-related-proteins-in-vitis-vinifera-thompson-seedless
#11
Li Jiao, Yali Zhang, Jiang Lu
Many U-box proteins have been identified and characterized as important factors against environmental stresses such as chilling, heat, salinity and pathogen attack in plant. Our previous research reported the cloning of a novel U-box protein gene VaPUB from Vitis amurensis 'Zuoshanyi' grape and suggested a function of it in related to cold stress in the model plant Arabidopsis system. In this study, the role of VaPUB in response to biotic and abiotic stress was further analyzed in the homologous grapevine system by studying the transcript regulation and the protein accumulation in VaPUB transgenic vines...
December 25, 2016: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28056396/transcriptome-profiling-of-sweetpotato-tuberous-roots-during-low-temperature-storage
#12
Chang Yoon Ji, Won-Hyong Chung, Ho Soo Kim, Won Yong Jung, Le Kang, Jae Cheol Jeong, Sang-Soo Kwak
Sweetpotato [Ipomoea batatas (L.) Lam] is a globally important root crop with high industrial value. However, because sweetpotato tuberous roots undergo chilling injuries that negatively affect their quality at temperatures below 10 °C, postharvest damage during the winter season is a major constraint for industrialization. To understand chilling injury response during postharvest low temperature storage, we used next-generation sequencing technology to comprehensive analyze the transcriptome of tuberous roots stored at optimal (13 °C) or low temperature (4 °C) for 6 weeks...
December 24, 2016: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28039815/differential-phenolic-production-in-leaves-of-vitis-vinifera-cv-alvarinho-affected-with-esca-disease
#13
Marta R M Lima, Mafalda L Felgueiras, Ana Cunha, Gisela Chicau, Federico Ferreres, Alberto C P Dias
Esca is a destructive disease of complex etiology affecting grapevines worldwide. A major constraint to the study and control of esca is that the disease is not diagnosed until external leaf and/or fruit symptoms are visible; however external symptoms usually appear several years after infection onset. We studied the phenolic content of V. vinifera cv. Alvarinho leaves using high performance liquid chromatography-diode array detection-mass spectrometry (HPLC-DAD-MS)/LC-MS. Leaves from affected cordons with and without visible symptoms (diseased and apparently healthy leaves, respectively) and leaves from asymptomatic cordons (healthy leaves) were analyzed...
December 24, 2016: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28088019/melatonin-and-resveratrol-reverse-the-toxic-effect-of-high-boron-b-and-modulate-biochemical-parameters-in-pepper-plants-capsicum-annuum-l
#14
Eleana Sarafi, Pavlos Tsouvaltzis, Christos Chatzissavvidis, Anastasios Siomos, Ioannis Therios
The objectives of this research were to test a possible involvement of melatonin (MEL) and resveratrol (RES) in restoring growth and to control boron (B) toxicity in peppers. The plants were subjected to four different nutrient solution treatments as following: 1) half-strength Hoagland's nutrient solution (Control), 2) half-strength Hoagland's nutrient solution+100 μM B (100 μMB), 3) half-strength Hoagland's nutrient solution+100 μM boron+100 μMresveratrol (100 μMRES), and 4) half-strength Hoagland's nutrient solution+100 μM B+1 μMmelatonin (1 μM MEL)...
December 23, 2016: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28033539/molecular-cloning-characterization-heterologous-expression-and-in-silico-analysis-of-disordered-boiling-soluble-stress-responsive-wbssrp-protein-from-drought-tolerant-wheat-cv-pbw-175
#15
Gurmeen Rakhra, Tarandeep Kaur, Dhiraj Vyas, Arun Dev Sharma, Jatinder Singh, Gobind Ram
The structural and physico-chemical properties that account for the multi-functionality of dehydrins remain largely unknown. In this study, we identified, sequenced and cloned a stress regulated cDNA encoding a dehydrin-like boiling stable protein (designated as wBsSRP; wheat boiling stable stress responsive protein) from drought stressed seedlings of drought tolerant cultivar of wheat (PBW 175). qRT-PCR analysis documented high transcripts levels of wBsSRP during drought and cold conditions in the tolerant cv...
December 23, 2016: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/27987474/temperature-and-co2-dependency-of-the-photosynthetic-photon-flux-density-responses-of-leaves-of-vitis-vinifera-cvs-chardonnay-and-merlot-grown-in-a-hot-climate
#16
Dennis H Greer
Comparisons of the photosynthetic responses to light and temperature between related cultivars are important to understand how well matched they are to the climate where they are grown. Photosynthetic light responses at a range of leaf temperatures and two CO2 concentrations were measured on leaves of two grapevine cultivars (Vitis vinifera L.) Chardonnay and Merlot vines growing in field conditions. The objective was to assess the interaction between photon flux density (PFD), leaf temperature and CO2 on photosynthesis and to compare the two cultivars...
December 13, 2016: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/27992771/comparative-analysis-of-cadmium-responsive-micrornas-in-roots-of-two-ipomoea-aquatica-forsk-cultivars-with-different-cadmium-accumulation-capacities
#17
Chuang Shen, Ying-Ying Huang, Chun-Tao He, Qian Zhou, Jing-Xin Chen, Xiao Tan, Samavia Mubeen, Jian-Gang Yuan, Zhong-Yi Yang
In plants, microRNAs (miRNAs) play regulatory roles in response to various environmental stresses. In order to illustrate the regulation mechanisms of miRNAs involving the different Cd accumulation abilities between a low-shoot-Cd cultivar (QLQ) and a high-shoot-Cd cultivar (T308) of water spinach (Ipomoea aquatic Forsk.), six sRNA libraries at 3 different time points were constructed. Only 5 miRNAs were exclusively regulated in QLQ, among them, miRNA395 was up-regulated, which was supposed to enhance the Cd retention and detoxification in root...
December 12, 2016: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/27992770/zinc-stress-affects-ionome-and-metabolome-in-tea-plants
#18
Yinfei Zhang, Yu Wang, Zhaotang Ding, Hui Wang, Lubin Song, Sisi Jia, Dexin Ma
The research of physiological responses to Zn stress in plants has been extensively studied. However, the ionomics and metabolomics responses of plants to Zn stress remain largely unknown. In present study, the nutrient elements were identified involved in ion homeostasis and metabolomics changes related to Zn deficiency or excess in tea plants. Nutrient element analysis demonstrated that the concentrations of Zn affected the ion-uptake in roots and the nutrient element transportation to leaves, leading to the different distribution of P, S, Al, Ca, Fe and Cu in the tea leaves or roots...
December 12, 2016: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/27987472/seed-priming-improves-chilling-tolerance-in-chickpea-by-modulating-germination-metabolism-trehalose-accumulation-and-carbon-assimilation
#19
Muhammad Farooq, Mubshar Hussain, Ahmad Nawaz, Dong-Jin Lee, Salem S Alghamdi, Kadambot H M Siddique
Chilling stress is one of the major abiotic stresses affecting chickpea productivity worldwide. This study evaluated the potential role of seed priming in improving resistance to chilling stress in chickpea (cv. Punjab, 2008). The priming treatments involved soaking seeds of chickpea cultivar Punjab 2008 in either water for 8 h (on-farm priming), aerated water (hydropriming) for 18 h, or CaCl2 solution (ψs -1.25 MPa; osmopriming) for 18 h. Primed and untreated seeds were grown either at 18/15 °C (control) or 13/10 °C (chilling stress)...
December 9, 2016: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/27987473/euphorbia-milii-native-bacteria-interactions-under-airborne-formaldehyde-stress-effect-of-epiphyte-and-endophyte-inoculation-in-relation-to-iaa-ethylene-and-ros-levels
#20
Gholamreza Khaksar, Chairat Treesubsuntorn, Paitip Thiravetyan
Better understanding of plant-bacteria interactions under stress is of the prime importance for enhancing airborne pollutant phytoremediation. No studies have investigated plant-epiphyte interactions compared to plant-endophyte interactions under airborne formaldehyde stress in terms of plant Indole-3-acetic acid (IAA), ethylene, reactive oxygen species (ROS) levels and pollutant removal efficiency. Euphorbia milii was inoculated with native plant growth-promoting (PGP) endophytic and epiphytic isolates individually to investigate plant-endophyte compared to plant-epiphyte interactions under continuous formaldehyde fumigation...
December 8, 2016: Plant Physiology and Biochemistry: PPB
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