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Journal of Fish Diseases

Cara L Brosnahan, John S Munday, Hye Jeong Ha, Mark Preece, John B Jones
A total of 777 fish from three growing regions of New Zealand Chinook salmon farms comprising of five sites were tested. Quantitative PCR was used to determine the distribution of New Zealand rickettsia-like organism and Tenacibaculum maritimum. Genetic information from these bacteria were then compared with strains reported worldwide. Using this information, suggested associations of pathogens with clinically affected fish were made. NZ-RLO was detected in two of the three regions, and T. maritimum was detected in all regions...
November 8, 2018: Journal of Fish Diseases
Shinya Mizuno, Shigehiko Urawa, Mahito Miyamoto, Makoto Hatakeyama, Nobuhisa Koide, Hiroshi Ueda
In northern Japan, juvenile chum salmon Oncorhynchus keta (Walbaum) are released from hatcheries to enhance the fishery resource. Infections with ectoparasitic protozoans, particularly the flagellate Ichthyobodo salmonis and the ciliate Trichodina truttae, occasionally cause severe mortality among hatchery-reared juveniles. This study examined the susceptibility of the two parasites to wide-ranging UV irradiation (experiment 1) and then investigated whether UV disinfection of the rearing water using a commercial device was useful for preventing infections among juveniles in a small-scale rearing system over a 28-day period (experiment 2)...
November 5, 2018: Journal of Fish Diseases
Sigríður Guðmundsdóttir, Niccoló Vendramin, Argelia Cuenca, Heiða Sigurðardóttir, Arni Kristmundsson, Tine Moesgaard Iburg, Niels Jørgen Olesen
A novel viral haemorrhagic septicaemia virus (VHSV) of genotype IV was isolated from wild lumpfish (Cyclopterus lumpus), brought to a land-based farm in Iceland, to serve as broodfish. Two groups of lumpfish juveniles, kept in tanks in the same facility, got infected. The virus isolated was identified as VHSV by ELISA and real-time RT-PCR. Phylogenetic analysis, based on the glycoprotein (G) gene sequences, may indicate a novel subgroup of VHSV genotype IV. In controlled laboratory exposure studies with this new isolate, there was 3% survival in the I...
November 5, 2018: Journal of Fish Diseases
Saengchan Senapin, Ha Thanh Dong, Watcharachai Meemetta, Warachin Gangnonngiw, Pakakul Sangsuriya, Rapeepun Vanichviriyakit, Molruedee Sonthi, Bunlung Nuangsaeng
In Southeast Asia, a new disease called scale drop disease (SDD) caused by a novel Megalocytivirus (SDDV) has emerged in farmed Asian sea bass (Lates calcarifer) in Singapore, Malaysia and Indonesia. We received samples from an Eastern Thai province that also showed gross signs of SDD (loss of scales). Clinical samples of 0.2-1.1 kg L. calcarifer collected between 2016 and 2018 were examined for evidence of SDDV infection. Histopathology was similar to that in the first report of SDDV from Singapore including necrosis, inflammation and nuclear pyknosis and karyorrhexis in the multiple organs...
November 5, 2018: Journal of Fish Diseases
Jun Xu, Yun-Dan Xie, Ling Liu, Song Guo, You-Lu Su, An-Xing Li
Streptococcus agalactiae is a major pathogen of tilapia causing significant economic losses for the global aquatic industry yearly. To elucidate the role of cel-EIIB protein-mediated phosphotransferase systems (PTS) in the virulence regulation of S. agalactiae, cel-EIIB gene deletion in a virulent strain THN0901 was achieved by homologous recombination. The cellobiose utilization of △cel-EIIB strain was significantly decreased relative to S.a.THN0901 strain incubating in LB with 10 mg/ml cellobiose (p < 0...
October 30, 2018: Journal of Fish Diseases
Jie Ma, Timothy J Bruce, Ponnerassery S Sudheesh, Christopher Knupp, Thomas P Loch, Mohamed Faisal, Kenneth D Cain
Bacterial coldwater disease, caused by Flavobacterium psychrophilum, remains one of the most significant bacterial diseases of salmonids worldwide. A previously developed and reported live-attenuated immersion vaccine (F. psychrophilum; B.17-ILM) has been shown to confer significant protection to salmonids. To further characterize this vaccine, a series of experiments were carried out to determine the cross-protective efficacy of this B.17-ILM vaccine against 9 F. psychrophilum isolates (representing seven sequence types/three clonal complexes as determined by multilocus sequence typing) in comparison with a wild-type virulent strain, CSF-259-93...
October 28, 2018: Journal of Fish Diseases
Magnus Vikan Røsaeg, Espen Rimstad, Arne Guttvik, Bård Skjelstad, Eldar Åsgard Bendiksen, Åse Helen Garseth
Salmonid alphavirus (SAV) causes pancreas disease (PD) in farmed Atlantic salmon (Salmo salar L.), and exocrine pancreas tissue is a primary target of the virus. Digestive enzymes secreted by the exocrine pancreas break down macromolecules in feed into smaller molecules that can be absorbed. The effect of SAV infection on digestion has been poorly studied. In this study, longitudinal observations of PD outbreaks caused by SAV subtype 2 (SAV2) in Atlantic salmon at two commercial sea sites were performed. The development of PD was assessed by measurement of SAV2 RNA load and evaluation of histopathological lesions typical of PD...
October 28, 2018: Journal of Fish Diseases
Heyong Jin Roh, Bo-Seong Kim, Ahran Kim, Nam Eun Kim, Yoonhang Lee, Won-Kyong Chun, Tho Diem Ho, Do-Hyung Kim
No abstract text is available yet for this article.
October 22, 2018: Journal of Fish Diseases
Jessica J Buss, Kathryn H Wiltshire, Thomas A A Prowse, James O Harris, Marty R Deveney
Bonamia spp. parasites threaten flat oyster (Ostrea spp.) farming worldwide. Understanding test performance is important for designing surveillance and interpreting diagnostic results. Following a pilot survey which found low Bonamia sp. intensity in farmed Ostrea angasi, we tested further oysters (n = 100-150) from each of three farms for Bonamia sp. using heart smear, histology and qPCR. We used a Bayesian Latent Class Model to assess diagnostic sensitivity (DSe) and specificity (DSp) of these tests individually or in combination, and to assess prevalence...
October 16, 2018: Journal of Fish Diseases
Yingying Wang, Qing Wang, Weiwei Zeng, Jiyuan Yin, Yingying Li, Yan Ren, Cunbin Shi, Sven M Bergmann, Xinping Zhu
Tilapia lake virus (TiLV) is an emerging disease threatening tilapia culture in many parts of the world. A cell line from the brain of tilapia, which was named TiB, was established, characterized and subcultured with more than 100 passages. The TiB cell line was optimally maintained at 27°C using medium 199 (M199) supplemented with 10% foetal bovine serum (FBS). Chromosome analysis revealed that 60% of TiB cells at passage 5 maintained the modal chromosome number 2n = 44, while at passage 60, there were 43% of TiB cells with the diploid chromosome number 2n = 50...
October 15, 2018: Journal of Fish Diseases
Rebecca Marie Ellul, Cecilie Walde, Gyri Teien Haugland, Heidrun Wergeland, Anita Rønneseth
The incidence of disease caused by Pasteurella sp. in farmed lumpsuckers in Norway has been steadily increasing in recent years, causing significant economic losses and fish welfare issues. The disease affects all life stages, both in hatcheries and after release into salmon cages. Therefore, it is important to establish robust challenge models, to be used for vaccine development. Exposure experiments via intramuscular and intraperitoneal injection underlined the high virulence of the bacteria, whereas the cohabitation and bath models allowed the chronic symptoms of the disease to be studied more accurately...
October 12, 2018: Journal of Fish Diseases
Jens-Erik Dessen, Turid Mørkøre, Jan Ivar Bildøy, Stig N Johnsen, Lars Thomas Poppe, Bjarne Hatlen, Magny S Thomassen, Kjell-Arne Rørvik
This study demonstrated that increased dietary protein-to-lipid ratio (P/L-ratio) improved survival of farmed Atlantic salmon naturally affected by pancreas disease (PD). In addition to diet, body weight (BW) and delousing mortality prior to the PD outbreak also contributed significantly (p < 0.05) to explain the observed variation in PD-associated mortality. Subsequent to the PD outbreak, large amount of fish failed to grow and caused thin fish with poor condition (runts). At the end of the trial, significantly (p < 0...
October 12, 2018: Journal of Fish Diseases
Julia Bauer, Felix Teitge, Lisa Neffe, Mikolaj Adamek, Arne Jung, Christina Peppler, Dieter Steinhagen, Verena Jung-Schroers
Due to their pathogenic potential, identifying Vibrio species from recirculating aquaculture systems (RAS) for Pacific white shrimp (Litopennaeus vannamei) is of great importance to determine the risk for animal's as well as for the consumer's health. The present study compared identification results for a total of 93 Vibrio isolates, including type strains and isolates from shrimp aquaculture. Results from biochemical identifications, 16S rRNA sequencing, sequencing of the uridylate kinase encoding gene pyrH and analysis of the protein spectra assessed by MALDI-TOF MS were compared...
October 12, 2018: Journal of Fish Diseases
Per Gunnar Fjelldal, Terje van der Meeren, Thomas W K Fraser, Florian Sambraus, Laith Jawad, Tom Johnny Hansen
Although spinal injuries in fish have been associated with electric stimuli applied during electrofishing and electrotrawling, bone fracture and repair in the axial skeleton have yet not been studied. To study this, we radiographed a group (n = 64) of individually tagged farmed cod twice, with a 1-year interval (∼36 cm at first and ∼ 50 cm at second inspection). The study focus was on the neural and haemal spines. These structures are un-paired and are not covered by other bones laterally, making them useful for radiological studies on axial skeletal fracture in live fish...
October 7, 2018: Journal of Fish Diseases
Laith A Jawad, Tom J Hansen, Sonnich Meier, Jon Egil Skjaeraasen, Per Gunnar Fjelldal
This study describes radiological malformations in the vertebral column of adult sexually mature wild haddock, Melanogrammus aeglefinus collected from Masfjorden, Western Norway, in 2014 and 2015. There were seven deformed fish (33% of sampled fish), five with mild deformities and two with severe deformities. The deformity types discovered were lordosis, vertebra body asymmetry, compression, fusion (ankylosis), remodelling, and haemal spine hyperostosis. The two severely deformed fish were as follows: (a) one specimen with 29 deformed vertebrae within the region vertebrae nos...
October 7, 2018: Journal of Fish Diseases
Refilwe Lukhwareni, Cobus van Dyk
This study aimed to assess the macroscopic appearance, histology and ultrastructure of liver nodular alterations in sharptooth catfish Clarias gariepinus (Burchell, 1822) from a hyper-eutrophic freshwater impoundment. The livers of 25 fish were sampled for microscopy analyses, otoliths were collected for age estimation, and the hepatosomatic index (HSI) was calculated. The light microscopic analysis of the nodular tissue revealed hepatocellular and nuclear pleomorphism, pyknotic nuclei, variation in cytoplasmic appearance and focal areas of cellular alterations (FCAs)...
October 7, 2018: Journal of Fish Diseases
I B Saticioglu, M Duman, T Wiklund, S Altun
Turkey was the largest rainbow trout producer of the European countries in 2016, and the reason for this production is mainly attributed to its egg and fry production. Flavobacterium psychrophilum cause the highest rates of mortality in the starting to feeding stages of the fish. In the present study, twenty-five F. psychrophilum isolates recovered from rainbow trout, coruh trout and brook trout were analysed by RAPD-PCR, ERIC-PCR, REP-PCR and PCR-RFLP, including 16S rRNA, gyrA and gyrB gene regions and PCR-based serotyping method...
October 7, 2018: Journal of Fish Diseases
Wanli Yan, Lide Gu, Shu Liu, Wei Ren, Mingsheng Lyu, Shujun Wang
Vibrio vulnificus is an important bacterial pathogen that causes serious infections in fish and is also highly pathogenic to humans. Many effective detection methods targeting this pathogen have previously been designed, but many of these methods are time-consuming, complicated and expensive. Thus, these approaches cannot be widely used by small aqacultural concerns. Although DNA aptamers have been used to detect pathogenic bacteria, these have not been applied to marine bacteria, including V. vulnificus. Therefore, we developed a highly specific DNA aptamer for V...
October 1, 2018: Journal of Fish Diseases
Chen-Fei Low, Mohd Radzi Md Yusoff, Giva Kuppusamy, Nur Farahiyah Ahmad Nadzri
Macrobrachium rosenbergii nodavirus (MrNV) has been threatening the giant freshwater prawn aquaculture since 1997, causing white tail disease in the prawn species that leads to 100% lethality of the infected postlarvae. Comprehension of the viral infectivity and pathogenesis at molecular biology level has recently resolved the viral capsid protein and evidenced the significant difference in the viral structural protein compared to other nodaviruses that infect fish and insect. Cumulative researches have remarked the proposal to assert MrNV as a member of new genus, gammanodavirus to the Nodaviridae family...
October 1, 2018: Journal of Fish Diseases
Monia Cocchi, Gabrita De Zan, Tiziana Di Giusto, Silvia Deotto, Tobia Pretto, Amedeo Manfrin, Romina Brunetta, Anna Toffan
No abstract text is available yet for this article.
September 26, 2018: Journal of Fish Diseases
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