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Journal of Fish Diseases

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https://www.readbyqxmd.com/read/27905123/selective-precipitation-reaction-a-novel-diagnostic-test-for-tissue-pathology-in-atlantic-salmon-salmo-salar-infected-with-salmonid-alphavirus-sav3
#1
M Braceland, J Tinsley, D Cockerill, R Bickerdike, M F McLoughlin, P D Eckersall
While investigating biomarkers for infection with salmonid alphavirus (SAV), the cause of pancreas disease (PD), a selective precipitation reaction (SPR) has been discovered in serum which could be an on-farm qualitative test and an in-laboratory quantitative assay for health assessments in aquaculture. Mixing serum from Atlantic salmon, Salmo salar, with SAV infection with a sodium acetate buffer caused a visible precipitation which does not occur with serum from healthy salmon. Proteomic examination of the precipitate has revealed that the components are a mix of muscle proteins, for example enolase and aldolase, along with serum protein such as serotransferrin and complement C9...
November 30, 2016: Journal of Fish Diseases
https://www.readbyqxmd.com/read/27882570/ultrastructural-and-biomolecular-detection-of-rickettsiales-like-organisms-in-tissues-of-rainbow-trout-with-red-mark-syndrome
#2
M Galeotti, M Manzano, P Beraldo, C Bulfon, G Rossi, D Volpatti, G E Magi
Red mark syndrome (RMS) and US strawberry disease (US SD) are skin disorders affecting rainbow trout farmed in Europe and USA. The disease etiology has not yet been established. In spite of specific investigations, identifying Rickettsia-like organism (RLO)- and Midichloria-like organism (MLO)-related DNA in affected individuals, these pathogens have never been observed. We performed histological, ultrastructural and biomolecular analysis on skin and spleen samples of trout with RMS. Examination by TEM revealed the presence of intracytoplasmic microorganisms resembling Rickettsiales within macrophages, fibroblasts and erythrocytes...
November 24, 2016: Journal of Fish Diseases
https://www.readbyqxmd.com/read/27859355/ballan-wrasse-labrus-bergylta-ascanius-is-not-susceptible-to-pancreas-disease-caused-by-salmonid-alphavirus-subtype-2-and-3
#3
M V Røsaeg, H Sindre, D Persson, O Breck, D Knappskog, A B Olsen, T Taksdal
No abstract text is available yet for this article.
November 18, 2016: Journal of Fish Diseases
https://www.readbyqxmd.com/read/27859350/evaluating-bath-treatment-effectiveness-in-the-control-of-sea-lice-burdens-on-atlantic-salmon-in-new-brunswick-canada
#4
R Gautam, R Vanderstichel, A S Boerlage, C W Revie, K L Hammell
The use of medicinal bath treatment for sea lice is becoming more common, due to increasing resistance to in-feed treatments with emamectin benzoate. Common treatment modalities in New Brunswick, Canada, include Salmosan administered by tarpaulin or wellboat, and Paramove administered by wellboat. In this study, we assessed the effectiveness of these treatment modalities in the field between 2010 and 2015 using a web-based sea lice data management system (Fish-iTrends(©) ). Effectiveness was evaluated for adult female (AF) and for pre-adult and adult male (PAAM) life stages separately...
November 18, 2016: Journal of Fish Diseases
https://www.readbyqxmd.com/read/27859349/development-of-in-situ-hybridization-and-real-time-pcr-assays-for-the-detection-of-hepatospora-eriocheir-a-microsporidian-pathogen-in-the-chinese-mitten-crab-eriocheir-sinensis
#5
Z F Ding, J Q Chen, J Lin, X S Zhu, G H Xu, R L Wang, Q G Meng, W Wang
A microsporidian parasite, Hepatospora eriocheir, is an emerging pathogen for the Chinese mitten crab Eriocheir sinensis. Currently, there is scant information about the way it transmits infection in the crustacean of commercial importance, including its pathogenesis, propagation and infection route in vivo. In this study, chromogenic in situ hybridization (ISH) and quantitative real-time PCR (qPCR) assays were developed to address this pressing need, and we provided an advance in the detection methods available...
November 18, 2016: Journal of Fish Diseases
https://www.readbyqxmd.com/read/27862001/can-water-disinfection-prevent-the-transmission-of-infectious-koi-herpesvirus-to-na%C3%A3-ve-carp-a-case-report
#6
S M Bergmann, E S Monro, J Kempter
Hygienic measures such as disinfection are important tools for the maintenance of fish health in aquaculture. While little information is available on the disinfection of water intended for fish containment, Huwa-San(®) , a disinfectant used in food and water industries, was used for daily treatment at concentrations of approximately 60 ppm over a total period of 3 months (experiment 1) with a 3-week treatment-free interval after 2 months (experiment 2). During this period, koi herpesvirus (KHV) was added to the water of two aquaria, one used as a normal contact control, the other one receiving daily water disinfectant treatments that prevented KHV infection of carp...
November 16, 2016: Journal of Fish Diseases
https://www.readbyqxmd.com/read/27747884/mycobacteria-in-aquarium-fish-results-of-a-3-year-survey-indicate-caution-required-in-handling-pet-shop-fish
#7
D Kušar, U Zajc, V Jenčič, M Ocepek, J Higgins, M Žolnir-Dovč, M Pate
Fish are commonly infected with non-tuberculous mycobacteria (NTM), which should be regarded as potential pathogens when handling aquarium fish and equipment. This study examined 107 aquarium fish from pet shops. Cultivation of the fish samples using different selective media was conducted for identification of NTM. Isolates were identified using the GenoType Mycobacterium common mycobacteria and additional species assays, sequencing of the 16S rRNA and rpoB genes, and real-time PCR assay for identification of Mycobacterium (M...
October 17, 2016: Journal of Fish Diseases
https://www.readbyqxmd.com/read/27734506/identification-and-characterization-of-a-phospholipase-a1-activity-type-three-secreted-protein-pp_exou-from-pseudomonas-plecoglossicida-nb2011-the-causative-agent-of-visceral-granulomas-disease-in-large-yellow-croaker-larimichthys-crocea
#8
J Zhang, Y Wang, H Guo, Z Mao, C Ge
Pseudomonas plecoglossicida NB2011, the causative agent of visceral granulomas disease in farmed Larimichthys crocea in China, encodes a predicted type three effector PP_ExoU, a homolog of the cytotoxin ExoU of Pseudomonas aeruginosa. In this study, secretion of PP_ExoU was tested in various broth, the protein was expressed with the pET30a prokaryotic system, the phospholipase A (PLA) activity of the recombinant protein was determined with fluorogenic phospholipid substrates, fusion expression with green fluorescent protein in transfected HeLa cells was investigated, and the lactate dehydrogenase (LDH) level was measured...
October 13, 2016: Journal of Fish Diseases
https://www.readbyqxmd.com/read/27734504/a-simplified-method-to-estimate-diphyllobothrium-spp-infection-in-salmonids
#9
J A Kuhn, R Knudsen, R Kristoffersen, P-A Amundsen
Some fish parasites constitute severe management problems as they may cause mortality of their fish host or are important zoonoses of humans. Parasite assessments are therefore critical to keep track of infections. If conventional sampling techniques can be simplified, parasite assessments might be easier to obtain, less time-consuming and more extensive. In this study, we compare the assessed number of Diphyllobothrium spp. cysts (CYST) with the counted number of Diphyllobothrium spp. plerocercoid larvae recovered using a conventional digestive technique (LARV)...
October 13, 2016: Journal of Fish Diseases
https://www.readbyqxmd.com/read/27734503/spondylosis-in-a-green-moray-eel-gymnothorax-funebris-ranzani-1839-with-swim-bladder-hyperinflation
#10
S M Boylan, A Camus, J Gaskins, J Oliverio, M Parks, A Davis, J Cassel
No abstract text is available yet for this article.
October 13, 2016: Journal of Fish Diseases
https://www.readbyqxmd.com/read/27723106/parasite-diversity-of-african-longfin-eel-anguilla-mossambica-peters-with-comments-on-host-response-to-the-monogenean-pseudodactylogyrus-anguillae-yin-and-sproston
#11
K J McHugh, O L F Weyl, N J Smit
No abstract text is available yet for this article.
October 10, 2016: Journal of Fish Diseases
https://www.readbyqxmd.com/read/27723105/arteriosclerosis-in-the-ventral-aorta-and-epicarditis-in-the-bulbus-arteriosus-of-atlantic-salmon-salmo-salar-l
#12
A S Dalum, K H Kristthorsdottir, D J Griffiths, K Bjørklund, T T Poppe
Spontaneous mortality of seemingly healthy, farmed Atlantic salmon (Salmo salar L) is an increasing problem in Norwegian aquaculture. In this study, we present a morphological study of the previously undescribed syndrome of arteriosclerosis of the ventral aorta and epicarditis of the adjacent bulbus arteriosus found in farmed Atlantic salmon, with wild-captured fish as a control group. Both the ventral aorta and epicardium are vital for correct arterial compliance and vascular resistance in the respiratory capillaries of the gills...
October 10, 2016: Journal of Fish Diseases
https://www.readbyqxmd.com/read/27696450/first-report-of-isolation-and-characterization-of-photobacterium-damselae-subsp-damselae-from-cage-farmed-cobia-rachycentron-canadum
#13
S R Krupesha Sharma, M A Pradeep, N Sadu, Praveen N Dube, K K Vijayan
No abstract text is available yet for this article.
October 3, 2016: Journal of Fish Diseases
https://www.readbyqxmd.com/read/27696444/investigation-of-epizootic-papillomatosis-in-bluegill-lepomis-macrochirus-rafinesque-1810-using-next-generation-sequencing
#14
J A Dill, S M Williams, J H Leary, T F F Ng, A C Camus
No abstract text is available yet for this article.
October 3, 2016: Journal of Fish Diseases
https://www.readbyqxmd.com/read/27690267/effect-of-feed-supplementation-with-kynurenic-acid-on-the-morphology-of-the-liver-kidney-and-gills-in-rainbow-trout-oncorhynchus-mykiss-walbaum-1792-healthy-and-experimentally-infected-with-yersinia-ruckeri
#15
E Kaczorek, J Szarek, M Mikiewicz, E Terech-Majewska, P Schulz, J Małaczewska, R Wójcik, A K Siwicki
Kynurenic acid (KYNA) is an endogenous substance produced on the kynurenine pathway which is primarily known for its neuroactive properties. Recently, it has been proven that KYNA is a selective ligand for G protein-coupled receptor (GPR 35), presented on immunocompetent cells such as T lymphocytes. This opens up new possibilities of its application as an immunostimulating substance in aquaculture. Thus far, no histopathological investigations in fish have been completed to evaluate influence of KYNA supplementation in feed...
September 30, 2016: Journal of Fish Diseases
https://www.readbyqxmd.com/read/27690177/antibiotic-resistance-and-repetitive-element-pcr-fingerprinting-in-aeromonas-veronii-isolates
#16
T-H Chung, S-W Yi, G-W Shin
This study evaluated antibiotic resistance and the related genes in total 47 Aeromonas veronii isolates from pet fish, eel (Anguilla japonica) and koi (Cyprinus carpio) in Korea. In comparison with the antibiotic susceptibilities of isolates from eel and koi, those of pet fish were more resistant to ceftiofur, aminoglycosides, tetracycline and nitrofurantoin. And isolates from pet fish showed high prevalences of class 1 integron, quinolones and tetracycline resistance determinants than those from eel and koi...
September 30, 2016: Journal of Fish Diseases
https://www.readbyqxmd.com/read/27670837/investigations-into-the-temporal-development-of-epitheliocystis-infections-in-brown-trout-a-histological-study
#17
M Guevara Soto, B Vidondo, L Vaughan, J-F Rubin, H Segner, S Samartin, H Schmidt-Posthaus
Epitheliocystis in Swiss brown trout (Salmo trutta) is a chlamydial infection, mainly caused by Candidatus Piscichlamydia salmonis and Candidatus Clavichlamydia salmonicola. To gain a better understanding of the temporal development of infections in wild brown trout, we investigated epitheliocystis infections during the course of the summer and autumn months of a single year (2015), and compared this to sampling points over the span of the years 2012-2014. The survey focused on tributaries (Venoge and Boiron) of the Rhone flowing in to Lake Geneva...
September 27, 2016: Journal of Fish Diseases
https://www.readbyqxmd.com/read/27670740/sensitivity-and-specificity-of-real-time-pcr-and-bacteriological-culture-for-francisellosis-in-farm-raised-nile-tilapia-oreochromis-niloticus-l
#18
G B N Assis, T F de Oliveira, I A Gardner, H C P Figueiredo, C A G Leal
Despite the worldwide occurrence of Francisella noatunensis subsp. orientalis (Fno) infection in farmed tilapia, sensitivity and specificity estimates of commonly used diagnostic tests have not been reported. This study aimed to estimate the sensitivity and specificity of bacteriological culture and qPCR to detect Fno infection. We tested 559 fish, sampled from four farms with different epidemiological scenarios: (i) healthy fish in a hatchery free of Fno; (ii) targeted sampling of diseased fish with suggestive external clinical signs of francisellosis during an outbreak; (iii) convenience sampling of diseased and clinically healthy fish during an outbreak; and (iv) sampling of healthy fish in a cage farm without a history of outbreaks, but with francisellosis reported in other farms in the same reservoir...
September 27, 2016: Journal of Fish Diseases
https://www.readbyqxmd.com/read/27667789/bacteriological-and-histopathological-study-of-farmed-rainbow-trout-oncorhynchus-mykiss-in-peru
#19
E C Mateo, T Castro, V Sierralta, D R Mateo
No abstract text is available yet for this article.
September 26, 2016: Journal of Fish Diseases
https://www.readbyqxmd.com/read/27717007/provisional-epidemiological-cutoff-values-for-standard-broth-microdilution-susceptibility-testing-of-flavobacterium-columnare
#20
C M Gieseker, T C Crosby, L C Woods
The gliding aquatic bacterium Flavobacterium columnare causes columnaris disease, a common problem for wild and farmed freshwater fish worldwide. Recently, a broth microdilution method was standardized to test the susceptibility of F. columnare against antimicrobials commonly used in aquaculture. We used this new method to measure the minimal inhibitory concentrations (MICs) of ten antimicrobials against 120 F. columnare isolates. The resulting MIC frequency distributions for each antimicrobial (1 MIC/isolate) were used to estimate epidemiological cut-off values (ECVs) which separate isolates with typical wild-type (WT) susceptibility from isolates with decreased non-wild-type (NWT) susceptibility...
September 22, 2016: Journal of Fish Diseases
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