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Journal of Fish Diseases

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https://www.readbyqxmd.com/read/28631828/development-of-a-colloidal-gold-immunochromatographic-assay-gica-for-the-rapid-detection-of-spiroplasma-eriocheiris-in-commercially-exploited-crustaceans-from-china
#1
H Liu, Y Xiu, Y Xu, M Tang, S Li, W Gu, Q Meng, W Wang
Spiroplasma eriocheiris is an emerging pathogen in freshwater crustaceans. In recent years, Eriocheir sinensis, Procambarus clarkii, Litopenaeus vannamei, Macrobrachium rosenbergii and Macrobrachium nipponense had been infected by this pathogen in China. An immunochromatographic strip test using gold nanoparticles was developed for rapidly detecting this pathogen. The strip test based on the principle of sandwich immunoassay by the specific combination between the pathogen and polyclonal antibody on a nitrocellulose membrane...
June 20, 2017: Journal of Fish Diseases
https://www.readbyqxmd.com/read/28631825/studies-on-the-occurrence-of-infectious-myonecrosis-virus-in-pond-reared-litopenaeus-vannamei-boone-1931-in-india
#2
A S Sahul Hameed, S Abdul Majeed, S Vimal, N Madan, T Rajkumar, S Santhoshkumar, S Sivakumar
Whiteleg shrimp, Litopenaeus vannamei, with clinical sign of muscle opaqueness with reddish colour at the distal abdominal segments were observed in farms located in West Bengal State, India. The mortality of shrimp in all disease outbreak ponds ranged from 20% to 50%, and mortality increased gradually. The RT-PCR assay of these samples using primer sets specific to infectious myonecrosis virus (IMNV) revealed its presence in the disease outbreak ponds. The IMNV infection was reproduced in healthy shrimp by intramuscular injection to satisfy River's postulates...
June 20, 2017: Journal of Fish Diseases
https://www.readbyqxmd.com/read/28590067/detection-of-tilapia-lake-virus-in-egyptian-fish-farms-experiencing-high-mortalities-in-2015
#3
P Nicholson, M A Fathi, A Fischer, C Mohan, E Schieck, N Mishra, A Heinimann, J Frey, B Wieland, J Jores
No abstract text is available yet for this article.
June 7, 2017: Journal of Fish Diseases
https://www.readbyqxmd.com/read/28581211/sickeningly-sweet-l-rhamnose-stimulates-flavobacterium-columnare-biofilm-formation-and-virulence
#4
M D Lange, B D Farmer, A M Declercq, E Peatman, A Decostere, B H Beck
Flavobacterium columnare, the causative agent of columnaris disease, causes substantial mortality worldwide in numerous freshwater finfish species. Due to its global significance and impact on the aquaculture industry continual efforts to better understand basic mechanisms that contribute to disease are urgently needed. The current work sought to evaluate the effect of L-rhamnose on the growth characteristics of F. columnare. While we initially did not observe any key changes during the total growth of F. columnare isolates tested when treated with L-rhamnose, it soon became apparent that the difference lies in the ability of this carbohydrate to facilitate the formation of biofilms...
June 5, 2017: Journal of Fish Diseases
https://www.readbyqxmd.com/read/28548705/aeromonas-sobria-an-outbreak-of-natural-infection-in-cultured-silver-catfish-rhamdia-quelen-quoy-gaimard-1824-in-argentina
#5
G A Coscelli, C Casabonne, E Morón-Alcain, N Arancegui, F A Vigliano
No abstract text is available yet for this article.
May 26, 2017: Journal of Fish Diseases
https://www.readbyqxmd.com/read/28548703/microsporidians-xenomas-of-anglerfish-from-ne-atlantic-waters
#6
M Llarena-Reino, E Abollo, S Pascual
The presence of emergent visible parasites at commercial valuable fish species is increasingly causing problems at fisheries and seafood industries. Microsporidians have been previously reported to appear forming apparent xenomas complexes in anglerfish species, but no effort has been carried out to simultaneously integrate epidemiological data, phenotypic, genotypic and fine structural characterizations in the same parasite sample. In this work, specimens of Lophius budegassa and Lophius piscatorius from NE Atlantic waters were sampled and examined to provide information about specific site of infection and demographic data of two groups of different sizes of xenomas present at both fish species...
May 26, 2017: Journal of Fish Diseases
https://www.readbyqxmd.com/read/28548700/efficacy-testing-of-35-year-old-commercially-produced-erm-bacterin-reveals-the-remarkable-stability-of-this-product
#7
T J Welch, T D Goodrich, S E LaPatra
No abstract text is available yet for this article.
May 26, 2017: Journal of Fish Diseases
https://www.readbyqxmd.com/read/28548691/first-identification-and-characterization-of-tenacibaculum-dicentrarchi-isolated-from-chilean-red-conger-eel-genypterus-chilensis-guichenot-1848
#8
R Irgang, R González-Luna, J Gutiérrez, M Poblete-Morales, V Rojas, D Tapia-Cammas, R Avendaño-Herrera
No abstract text is available yet for this article.
May 26, 2017: Journal of Fish Diseases
https://www.readbyqxmd.com/read/28548690/rainbow-trout-oncorhynchus-mykiss-immune-response-towards-a-recombinant-vaccine-targeting-the-parasitic-ciliate-ichthyophthirius-multifiliis
#9
L von Gersdorff Jørgensen, P W Kania, K J Rasmussen, A H Mattsson, J Schmidt, A Al-Jubury, A Sander, A Salanti, K Buchmann
The protective effect in rainbow trout (Oncorhynchus mykiss) of an experimental subunit vaccine targeting antigens in the parasite Ichthyophthirius multifiliis has been evaluated and compared to effects elicited by a classical parasite homogenate vaccine. Three recombinant parasite proteins (two produced in E. coli and one in insect cells) were combined and injected i.p., and subsequently, protection and antibody responses were analysed. Both the experimental and the benchmark vaccine induced partial but significant protection against I...
May 26, 2017: Journal of Fish Diseases
https://www.readbyqxmd.com/read/28548689/phenotype-of-aeromonas-salmonicida-sp-salmonicida-cyclic-adenosine-3-5-monophosphate-receptor-protein-crp-mutants-and-its-virulence-in-rainbow-trout-oncorhynchus-mykiss
#10
K Valderrama, M Saravia, J Santander
Precise deletion of genes related to virulence can be used as a strategy to produce attenuated bacterial vaccines. Here, we study the deletion of the cyclic-3',5'-adenosine monophosphate (cAMP) receptor protein (Crp) in Aeromonas salmonicida, the aetiologic agent of furunculosis in marine and freshwater fish. The Crp protein is a conserved global regulator, controlling physiology processes, like sugar utilization. Deletion of the crp gene has been utilized in live attenuated vaccines for mammals, birds and warm water fish...
May 26, 2017: Journal of Fish Diseases
https://www.readbyqxmd.com/read/28548687/first-report-of-red-mark-syndrome-rms-in-farmed-rainbow-trout-in-slovenia
#11
M Galeotti, P Ronza, P Beraldo, C Bulfon, G E Magi, M Manzano, D Volpatti
No abstract text is available yet for this article.
May 26, 2017: Journal of Fish Diseases
https://www.readbyqxmd.com/read/28548686/studies-on-the-antibody-response-and-side-effects-after-intramuscular-and-intraperitoneal-injection-of-atlantic-lumpfish-cyclopterus-lumpus-l-with-different-oil-based-vaccines
#12
T Erkinharju, M R Lundberg, E Isdal, I Hordvik, R A Dalmo, T Seternes
Atlantic lumpfish (Cyclopterus lumpus L.) is used as a biological delousing agent for sea lice (Lepeophtheirus salmonis K.) infestations in Norwegian aquaculture. Here, we present a study on the antibody response and vaccine side effects after intramuscular and intraperitoneal injection of lumpfish with two vaccines. Both vaccines contained bacterial antigens from atypical Aeromonas salmonicida A-layer types V and VI, Vibrio anguillarum serotype O1 and Moritella viscosa sp., but one vaccine contained a vegetable oil-based adjuvant, while the other contained a mineral oil-based adjuvant...
May 26, 2017: Journal of Fish Diseases
https://www.readbyqxmd.com/read/28548685/detection-methods-of-cyprinid-herpesvirus-2-infection-in-silver-crucian-carp-carassius-auratus-gibelio-via-a-porf72-monoclonal-antibody
#13
S Y Kong, Y S Jiang, Q Wang, J F Lu, D Xu, L Q Lu
Cyprinid herpesvirus 2 (CyHV-2) is the main pathogen responsible for causing haematopoietic necrosis disease in Carassius auratus gibelio. Although many nucleic acid-based diagnostic methods have been applied, no stable and sensitive immunological diagnostic approaches have been reported. In this study, to detect CyHV-2 in clinical samples using immunological methods, recombinant ORF72 protein (pORF72), encoded by the CyHV-2 ORF72 gene, was used as a capture antigen to identify blood and tissues infected with CyHV-2...
May 26, 2017: Journal of Fish Diseases
https://www.readbyqxmd.com/read/28524261/application-of-low-frequency-sonophoresis-and-reduction-of-antibiotics-in-the-aquatic-systems
#14
C Cobo Labarca, J Radinger, V Schöning, R Ariav, R Jung, K D Thompson, W Kloas, K Knopf
A major concern in aquaculture is the use of chemical therapeutics, such as antibiotics, because of their impact on the environment as well as on the fish product. As a potential tool for reducing antibiotic use, we tested the application of low-frequency ultrasound as a method for enhancing antibiotic uptake. Rainbow trout juveniles (Oncorhynchus mykiss) were exposed to two different concentrations of oxytetracycline (OTC), flumequine (FLU) and florfenicol (FLO), administered by bath after the application of ultrasound...
May 19, 2017: Journal of Fish Diseases
https://www.readbyqxmd.com/read/28493514/protective-effect-and-antibody-response-of-dna-vaccine-against-salmonid-alphavirus-3-sav3-in-atlantic-salmon
#15
C J Chang, J Gu, B Robertsen
This work reports the effect of two DNA vaccines against salmonid alphavirus 3 (SAV3) in Atlantic salmon. Presmolts were vaccinated by intramuscular injection of plasmids encoding the SAV3 structural polyprotein C-E3-E2-6K-E2 (pCSP), E2 only (pE2), or plasmid without insert (pcDNA3.3). E2 is expressed at the surface of cells transfected with pCSP and internally in cells transfected with pE2. A commercial vaccine based on inactivated SAV (NCPD) was used for comparison. At 10 weeks post-vaccination, only fish vaccinated with pCSP showed antibody against E2 and virus-neutralizing activity...
May 11, 2017: Journal of Fish Diseases
https://www.readbyqxmd.com/read/28493503/liver-of-the-fish-gymnotus-inaequilabiatus-and-nematode-larvae-infection-histochemical-features-and-expression-of-proliferative-cell-nuclear-antigen
#16
B Sayyaf Dezfuli, M Manera, J A DePasquale, F Pironi, L Giari
Histopathological lesions due to third-larval stage of nematode Brevimulticaecum sp. within the liver of a subpopulation of 31 Gymnotus inaequilabiatus from the Pantanal Region (Brazil) were studied with histochemical and immunohistochemical methods. In 93.5% of fish, livers harboured nematode larvae and the intensity of infection ranged from 8 to 293. In livers with highest number of larvae, the hepatic tissue was occupied primarily by the nematodes. Each larva was encircled by focal inflammatory granulomatous reaction...
May 11, 2017: Journal of Fish Diseases
https://www.readbyqxmd.com/read/28493490/the-protective-effect-of-humic-rich-substances-on-atypical-aeromonas-salmonicida-subsp-salmonicida-infection-in-common-carp-cyprinus-carpio-l
#17
G Yamin, R Falk, R R Avtalion, N Shoshana, T Ofek, R Smirnov, G Rubenstein, J van Rijn
When challenged with atypical Aeromonas salmonicida subsp. salmonicida, exposure of the common carp (Cyprinus carpio L.) to different humic-rich compounds resulted in a significant reduction in infection rates. Specifically, in fish exposed to (i) humic-rich water and sludge from a recirculating system, (ii) a synthetic humic acid, and (iii) a Leonardite-derived humic-rich extract, infection rates were reduced to 14.9%, 17.0% and 18.8%, respectively, as compared to a 46.8% infection rate in the control treatment...
May 11, 2017: Journal of Fish Diseases
https://www.readbyqxmd.com/read/28493487/effect-of-starvation-and-refeeding-on-the-hepatopancreas-of-whiteleg-shrimp-penaeus-vannamei-boone-using-computer-assisted-image-analysis
#18
F Cervellione, C McGurk, T Berger Eriksen, W Van den Broeck
Under normal farming conditions, shrimp can experience starvation periods attributable to disease outbreaks or adverse environmental conditions. Starvation leads to significant morphological changes in the hepatopancreas (HP), being the main organ for absorption and storage of nutrients. In the literature, limited research has described the effect on the HP of periods of starvation followed by refeeding and none in whiteleg shrimp (Penaeus vannamei) using computer-assisted image analysis (CAIA). This study describes the effect of starvation and starvation followed by refeeding on the HP of whiteleg shrimp using CAIA...
May 11, 2017: Journal of Fish Diseases
https://www.readbyqxmd.com/read/28493486/pansteatitis-in-polluted-olifants-river-impoundments-nutritional-perspectives-on-fish-in-a-eutrophic-lake-lake-loskop-south-africa
#19
K D A Huchzermeyer, S Woodborne, G Osthoff, A Hugo, A C Hoffman, H Kaiser, J C A Steyl, J G Myburgh
This study compares the aetiology of pansteatitis in Lake Loskop, relative to two other impoundments along the Olifants River. Macroscopic and microscopic pathology, age determination and analysis of stomach content, fatty acids and stable isotopes explain the high prevalence of pansteatitis in Oreochromis mossambicus (Peters) and several other species in Lake Loskop. All the dietary indicator comparisons between pansteatitis-affected and healthy fish fail to support a systemic cause. Pansteatitis in Lake Loskop was linked to size and weight of O...
May 11, 2017: Journal of Fish Diseases
https://www.readbyqxmd.com/read/28470973/validation-of-a-khv-antibody-enzyme-linked-immunosorbent-assay-elisa
#20
S M Bergmann, Q Wang, W Zeng, Y Li, Y Wang, M Matras, M Reichert, D Fichtner, M Lenk, T Morin, N J Olesen, H F Skall, P-Y Lee, S Zheng, S Monaghan, S Reiche, W Fuchs, M Kotler, K Way, G Bräuer, K Böttcher, A Kappe, J Kielpinska
Koi herpesvirus (KHV) causes KHV disease (KHVD). The virus is highly contagious in carp or koi and can induce a high mortality. Latency and, in some cases, a lack of signs presents a challenge for virus detection. Appropriate immunological detection methods for anti-KHV antibodies have not yet been fully validated for KHV. Therefore, it was developed and validated an enzyme-linked immunosorbent assay (ELISA) to detect KHV antibodies. The assay was optimized with respect to plates, buffers, antigens and assay conditions...
May 4, 2017: Journal of Fish Diseases
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