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Journal of Fish Diseases

T Jagielski, M Dyląg, U Roesler, J Murugaiyan
Protothecosis is a rare infection caused by environmentally ubiquitous achlorophyllic microalgae of the genus Prototheca. Here, we describe a first case of protothecosis in a carp (Cyprinus carpio), which is at the same time the first case of protothecosis in a fish, confirmed by phenotype- and molecular-based methods, including PCR sequencing of the rDNA cluster and protein profiling using matrix-assisted laser desorption ionization time-of-flight mass spectrometry.
March 16, 2017: Journal of Fish Diseases
S L Semple, N T K Vo, A R Li, P H Pham, N C Bols, B Dixon
Arctic charr (Salvelinus alpinus) are the northernmost distributed freshwater fish and can grow at water temperatures as low as 0.2 °C. Other teleost species have impaired immune function at temperatures that Arctic charr thrive in, and thus, charr may maintain immune function at these temperatures. In this study, a fibroblastic cell line, named ACBA, derived from the bulbus arteriosus (BA) of Arctic charr was developed for use in immune studies at various temperatures. ACBA has undergone more than forty passages at 18 °C over 3 years, while showing no signs of senescence-associated β-galactosidase activity and producing nitric oxide...
March 6, 2017: Journal of Fish Diseases
J Wiik-Nielsen, M Gjessing, H T Solheim, A Litlabø, A-G Gjevre, A B Kristoffersen, M D Powell, D J Colquhoun
Elucidation of the role of infectious agents putatively involved in gill disease is commonly hampered by the lack of culture systems for these organisms. In this study, a farmed population of Atlantic salmon pre-smolts, displaying proliferative gill disease with associated Candidatus Branchiomonas cysticola, Ca. Piscichlamydia salmonis and Atlantic salmon gill pox virus (SGPV) infections, was identified. A subpopulation of the diseased fish was used as a source of waterborne infection towards a population of naïve Atlantic salmon pre-smolts...
March 6, 2017: Journal of Fish Diseases
S E Ali, I Skaar
The incidence of fish pathogenic oomycetes, Saprolegnia, has increased significantly in aquaculture since the ban of malachite green. For the efficient characterization of anti-Saprolegnia therapeutics, simple accurate methods are required. However, the current screening methods are limited by time, and none of them are confirming the viability of treated spores or hyphae. In this study, a modified fluorescence-based assay for the in vitro screening of Saprolegnia inhibitors has been developed. This method involves the use of FUN-1 viability dye combined with calcofluor white M2R, and is based on the formation of orange-red cylindrical intravacuolar structures (CIVS) in metabolically active spores, hyphae and biofilms...
March 2, 2017: Journal of Fish Diseases
G M Shen, C Y Shi, C Fan, D Jia, S Q Wang, G S Xie, G Y Li, Z L Mo, J Huang
The hybrid grouper, Epinephelus fuscoguttatus (♀) × Epinephelus lanceolatus (♂), is a newly bred cultivated marine fish species of high economic value. However, a skin ulcer disease with high mortality has occurred, and the responsible pathogen remains unknown. In this study, we summarized the epidemic status and external signs of this disease. We screened potential pathogens and finally isolated one bacterial strain ML01 from affected fish. We subjected healthy juvenile hybrid groupers to bacterial challenge tests with the isolate by immersion, immersion after dermal abrasion and intraperitoneal injection, respectively...
March 2, 2017: Journal of Fish Diseases
C-F Low, M Z H Rozaini, N Musa, B Syarul Nataqain
The approaches of transcriptomic and proteomic have been widely used to study host-pathogen interactions in fish diseases, and this is comparable to the recently emerging application of metabolomic in elucidating disease-resistant mechanisms in fish that gives new insight into potential therapeutic strategies to improve fish health. Metabolomic is defined as the large-scale study of all metabolites within an organism and represents the frontline in the 'omics' approaches, providing direct information on the metabolic responses and perturbations in metabolic pathways...
March 2, 2017: Journal of Fish Diseases
A Doszpoly, I M Kalabekov, R Breyta, I S Shchelkunov
Siberian sturgeon herpesvirus (SbSHV) was isolated in Russia for the first time in 2006. Nine SbSHV isolates were recovered from different fish hatcheries producing the same cytopathic effect in cell cultures, the same clinical signs and mortality kinetics in virus-infected fish and the same virus neutralization pattern and shared identical nucleotide sequences. In 2011, a new isolate was recovered from juvenile sturgeon, which caused completely different cytopathic effect. That isolate was not readily neutralized by Siberian sturgeon hyperimmune antisera, and its DNA was not recognized by the routine PCR developed for SbSHV detection...
February 27, 2017: Journal of Fish Diseases
A Papadopoulou, I Dalsgaard, A Lindén, T Wiklund
The adherence of Flavobacterium psychrophilum to surfaces of epithelial tissues has been inconclusively suggested as a mechanism, which enables the bacterium to invade the host. Hence, the present study aimed to examine the adherence of the cells of two colony phenotypes, smooth and rough, of F. psychrophilum to mucosal tissues of rainbow trout fry and to test the skin mucus as a nutrient for the growth of F. psychrophilum. Fish were immersed in water containing 10(6 ) CFU( ) mL(-1) F. psychrophilum for each colony phenotype...
February 11, 2017: Journal of Fish Diseases
F Sambraus, P G Fjelldal, S C Remø, E M Hevrøy, T O Nilsen, A Thorsen, T J Hansen, R Waagbø
The aim of the present study was to investigate cataract development in diploid (2N) and triploid (3N) Atlantic salmon smolts and post-smolts at two water temperatures (10 and 16 °C) given diets with different histidine supplementation (LH, 10.4 and HH, 13.1 g kg(-1) ) before and after seawater transfer. In freshwater, a severe cataract outbreak was recorded in both ploidies reared at 16 °C. The cataract score was significantly higher in triploids compared to diploids, and the severity was lower in both ploidies fed the HH diet...
February 11, 2017: Journal of Fish Diseases
A Rønneseth, D Castillo, P D'Alvise, Ø Tønnesen, G Haugland, T Grotkjaer, K Engell-Sørensen, L Nørremark, Ø Bergh, H I Wergeland, L Gram
Vibrionaceae infections are a major obstacle for marine larviculture; however, little is known about virulence differences of Vibrio strains. The virulence of Vibrio strains, mostly isolated from vibriosis outbreaks in farmed fish, was tested in larval challenge trials with cod (Gadus morhua), turbot (Scophthalmus maximus) and halibut (Hippoglossus hippoglossus) using a multiwell dish assays with single-egg/larvae cultures. The strains differed significantly in virulence as some caused a high mortality of larva reaching 100% mortality after a few days, while others had no or only marginal effects on survival...
February 4, 2017: Journal of Fish Diseases
F Lagos, C Cartes, T Vera, D Haussmann, J Figueroa
Piscirickettsia salmonis, an agent of Piscirickettsiosis, is the cause of major losses in the Chilean salmon industry. We identified, characterized and bioinformatically analysed genomic islands in field strains of P. Salmonis, using the bioinformatic software PIPS, that uses the characteristics of the islands of pathogenicity to identify them. We analysed nine partially sequenced genomes in different new field strains, and compared them with the LF-89 (Type strain) genome, selecting a genomic island present in all of them...
February 2, 2017: Journal of Fish Diseases
D Jaramillo, P Hick, R J Whittington
Age-dependent susceptibility to nervous necrosis virus (NNV) was demonstrated for barramundi (Lates calcarifer). The experiment used juvenile barramundi produced from a single spawning that were challenged consecutively by immersion with a redspotted grouper nervous necrosis virus (RGNNV) isolate. The dose and environmental conditions (35 ppt salinity and 30 °C) were constant. Fish and water were sampled longitudinally for histopathology and RT-qPCR analysis to examine the evolution of the disease, virus replication, immune response and release of virus into water...
January 24, 2017: Journal of Fish Diseases
P Kayansamruaj, A Rangsichol, H T Dong, C Rodkhum, M Maita, T Katagiri, N Pirarat
In 2013, an outbreak of ulcerative disease associated with ranavirus infection occurred in barcoo grunter, Scortum barcoo (McCulloch & Waite), farms in Thailand. Affected fish exhibited extensive haemorrhage and ulceration on skin and muscle. Microscopically, the widespread haemorrhagic ulceration and necrosis were noted in gill, spleen and kidney with the presence of intracytoplasmic eosinophilic inclusion bodies. When healthy barcoo grunter were experimentally challenged via intraperitoneal and oral modes with homogenized tissue of naturally infected fish, gross and microscopic lesions occurred with a cumulative mortality of 70-90%...
January 23, 2017: Journal of Fish Diseases
A T Vincent, S J Charette
No abstract text is available yet for this article.
January 20, 2017: Journal of Fish Diseases
E Lari, G G Pyle
Monogenean worms are ectoparasites that are known to be infectious to a wide variety of fish. Few species of monogenean parasites have been reported in the olfactory chamber of fish in current peer-reviewed literature. However, the impacts of these parasites on the olfactory system are not well understood. In this study, the effects of Gyrodactylus salmonis on the olfactory system structure and performance were investigated in rainbow trout (Oncorhynchus mykiss). The olfactory performance of the infected fish was examined using an electro-olfactography (EOG) technique, while the ultrastructure of the olfactory rosette was studied using scanning electron microscopy (SEM) and light microscopy (LM)...
January 20, 2017: Journal of Fish Diseases
M C Gjessing, E Thoen, T Tengs, S A Skotheim, O B Dale
Gill diseases cause considerable losses in Norwegian salmon farming. In 2015, we characterized salmon gill poxvirus (SGPV) and associated gill disease. Using newly developed diagnostic tools, we show here that SGPV infection is more widely distributed than previously assumed. We present seven cases of complex gill disease in Atlantic salmon farmed in seawater and freshwater from different parts of Norway. Apoptosis, the hallmark of acute SGPV infection, was not easily observed in these cases, and qPCR analysis was critical for identification of the presence of SGPV...
January 20, 2017: Journal of Fish Diseases
R Knudsen, E H Henriksen, K Ø Gjelland, H Hansen, D K Hendrichsen, R Kristoffersen, K Olstad
The monogenean parasite Gyrodactylus salaris poses serious threats to many Atlantic salmon populations and presents many conservation and management questions/foci and challenges. It is therefore critical to identify potential vectors for infection. To test whether hybrids of native Atlantic salmon (Salmo salar) × brown trout (Salmo trutta) are suitable as reservoir hosts for G. salaris during winter, infected hybrid parr were released into a natural subarctic brook in the autumn. Six months later, 23.9% of the pit-tagged fish were recaptured...
January 20, 2017: Journal of Fish Diseases
M N Yousaf, T T Poppe
No abstract text is available yet for this article.
January 20, 2017: Journal of Fish Diseases
R Castro, M Reguera-Brito, G H López-Campos, M M Blanco, M Aguado-Urda, J F Fernández-Garayzábal, A Gibello
Lactococcus garvieae is the aetiological agent of lactococcosis, a haemorrhagic septicaemia that affects marine and freshwater fish, with special incidence and economic relevance in farmed rainbow trout. Water temperature is one of the most important predisposing factors in the development of lactococcosis outbreaks. Lactococcosis in trout usually occur when water temperatures rise to about 18 °C, while fish carriers remain asymptomatic at temperatures below 13 °C. The aim of this work was to analyse the differences in the complete transcriptome response of L...
January 17, 2017: Journal of Fish Diseases
J Dubert, J A Aranda-Burgos, J Ojea, J L Barja, S Prado
Diseases caused by bacteria belonging to the genus Vibrio are a common, as yet unresolved, cause of mortality in shellfish hatcheries. In this study, we report the results of routine microbiological monitoring of larval cultures of the carpet shell clam Ruditapes decussatus in a hatchery in Galicia (NW Spain). Previous episodes of mortality with signs similar to those of vibriosis affecting other species in the installation indicated the possibility of bacterial infection and led to division of the culture at the early D-veliger larval stage...
January 17, 2017: Journal of Fish Diseases
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