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Journal of Fish Diseases

H Y Pao, C Y Wu, C H Huang, C M Wen
A continuous cell line consisting mostly of epithelioid cells was established from the caudal fin of marbled eels (Anguilla marmorata) and designated as marbled eel caudal fin (MECF)-1. The cells multiplied well in Leibovitz's L-15 medium containing 2% to 15% foetal bovine serum at temperatures of 20°C to 35°C and were subcultured for >90 passages during a 5-year period from 2012 to 2017. Transcripts of ictacalcin, keratin 13, cd146, nestin, ncam1 and myod1 were demonstrated in the cells using reverse transcription polymerase chain reaction...
July 13, 2018: Journal of Fish Diseases
Ying-Fei Yang, Tien-Hsuan Lu, Hsing-Chieh Lin, Chi-Yun Chen, Chung-Min Liao
A novel virus, tilapia lake virus (TiLV), has been identified as a key pathogen responsible for disease outbreak and mass mortality of farmed tilapia. We used a deterministic susceptible-infectious-mortality (SIM) model to derive key disease information appraised with published TiLV-induced cumulative mortality data. The relationship between tilapia mortality and TiLV exposure dosages was described by the Hill model. Furthermore, a disease control model was proposed to determine the status of controlled TiLV infection using a parsimonious control reproduction number (RC )-control line criterion...
July 12, 2018: Journal of Fish Diseases
Liang Zhang, Zhuying Gao, Li Yu, Bo Zhang, Jing Wang, Jun Zhou
Nucleotide-binding and oligomerization domain (NOD)-like receptors (NLRs) are a group of intracellular pathogen recognition receptors (PRRs) that play key roles in pathogen recognition and subsequent activation of innate immune signalling pathways. Expressions of several NLR subfamily members, including NOD1, NOD2, NLR-C3, NLR-C5 and NLR-X1 have been reported in many different teleost fish species. These receptors are activated by a variety of ligands, including lipopolysaccharides (LPS), peptidoglycans (PGN) and polyinosinic-polycytidylic acid [Poly(I:C)]...
June 29, 2018: Journal of Fish Diseases
Vladimir López, María Angeles Risalde, Marinela Contreras, Lourdes Mateos-Hernández, Joaquin Vicente, Christian Gortázar, José de la Fuente
Control of mycobacterial infection constitutes a priority for human and animal health worldwide. However, effective vaccines are needed for the control of human and animal tuberculosis (TB). Adult zebrafish have become a useful model for studying the pathophysiology of mycobacterial infection and for the development of novel interventions for TB control and prevention. Recently, parenteral and oral immunization with the heat-inactivated Mycobacterium bovis vaccine (M. bovis IV) protected wild boar against TB...
June 29, 2018: Journal of Fish Diseases
Jiayuan Chen, Wenchao Wang, Xiuhua Wang, Qingli Zhang, Yanbei Ren, Jipeng Song, Xuepeng Wang, Xuan Dong, Jie Huang
No abstract text is available yet for this article.
June 25, 2018: Journal of Fish Diseases
Daniel William Wright, Barbara Nowak, Frode Oppedal, Phil Crosbie, Lars Helge Stien, Tim Dempster
Freshwater bathing is one of the main treatment options available against amoebic gill disease (AGD) affecting multiple fish hosts in mariculture systems. Prevailing freshwater treatments are designed to be long enough to kill Neoparamoeba perurans, the ectoparasite causing AGD, which may select for freshwater tolerance. Here, we tested whether using shorter, sublethal freshwater treatment durations are a viable alternative to lethal ones for N. perurans (2-4 hr). Under in vitro conditions, gill-isolated N...
June 25, 2018: Journal of Fish Diseases
Francis LeBlanc, Steven Leadbeater, Mark Laflamme, Nellie Gagné
The infectious salmon anaemia virus (ISAV) is capable of causing a significant disease in Atlantic salmon, which has resulted in considerable financial losses for salmon farmers around the world. Since the first detection of ISAV in Canada in 1996, it has been a high priority for aquatic animal health management and surveillance programmes have led to the identification of many genetically distinct ISAV isolates of variable virulence. In this study, we evaluated the virulence of three ISAV isolates detected in Atlantic Canada in 2012 by doing in vivo-controlled disease challenges with two sources of Atlantic salmon...
June 25, 2018: Journal of Fish Diseases
Bikramjit Ghosh, Philip B B Crosbie, Barbara F Nowak, Andrew R Bridle
Finfish with asymptomatic Yersinia ruckeri infections pose a major risk as they can transmit the pathogen and cause clinical outbreaks in stock populations. Current tools have insufficient quantitative ability for accurately detecting the trace levels of Y. ruckeri typically associated with asymptomatic infection, necessitate invasive or lethal sampling, or require long processing times. This study presents a highly sensitive qPCR-based method, targeting part of the Y. ruckeri 16S rRNA sequence, that is capable of detecting extremely low levels of Y...
June 21, 2018: Journal of Fish Diseases
Øystein Wessel, Øyvind Haugland, Marit Rode, Børge N Fredriksen, Maria K Dahle, Espen Rimstad
Heart- and skeletal muscle inflammation (HSMI) caused by infection with Piscine orthoreovirus (PRV) is one of the most common viral diseases in farmed Atlantic salmon (Salmo salar) in Norway, and disease outbreaks have been reported in most countries with large-scale Atlantic salmon aquaculture. Currently there is no vaccine available for protection against HSMI, partly due to the lack of a cell line for efficient virus propagation. Erythrocytes are the primary target cells for PRV in vivo and a potential source for isolation of PRV particles...
June 21, 2018: Journal of Fish Diseases
Marta Polinas, Salvatore Mele, Francesc Padrós, Paolo Merella, Elisabetta Antuofermo, Adam Gouraguine, Olga Reñones
The dusky grouper Epinephelus marginatus (Lowe) is an ecologically and commercially important fish species of the Atlantic and Mediterranean coastal rocky habitats. Despite records of didymozoid infections in several grouper species, the identification and pathogenesis of these parasites in E. marginatus are lacking. The aim of this study is to characterize the didymozoids of E. marginatus, particularly their mechanisms of infection and histopathological features. Dusky groupers (n = 205) were caught off Majorca Island (western Mediterranean Sea) and examined for parasites...
June 21, 2018: Journal of Fish Diseases
Craig A Shoemaker, Benjamin R LaFrentz, Eric Peatman, Benjamin H Beck
Flavobacterium columnare causes columnaris disease of farmed and wild freshwater fish. Skin mucus is an important factor in early stages of columnaris pathogenesis, albeit little studied. Our objectives were to (a) characterize the terminal glycosylation pattern (TGP) of catfish mucus, (b) determine the growth of F. columnare in formulated water (FW)-containing channel catfish (Ictalurus punctatus) or hybrid catfish (Ictalurus punctatus X Ictalurus furcatus) mucus and (c) examine extracellular protease activity of two F...
June 11, 2018: Journal of Fish Diseases
Åse Helen Garseth, Eirik Biering
No abstract text is available yet for this article.
June 11, 2018: Journal of Fish Diseases
Michael J MacLeod, Nguyen T K Vo, Michael S Mikhaeil, S Richelle Monaghan, J Andrew N Alexander, Mandeep K Saran, Lucy E J Lee
In vitro cell culture methods are crucial for the isolation, purification and mass propagation of intracellular pathogens of aquatic organisms. Cell culture infection models can yield insights into infection mechanisms, aid in developing methods for disease mitigation and prevention, and inform commercial-scale cultivation approaches. This study details the establishment of a larval cell line (GML-5) from the Atlantic cod (Gadus morhua) and its use in the study of microsporidia. GML-5 has survived over 100 passages in 8 years of culture...
June 8, 2018: Journal of Fish Diseases
Estelle Rousselet, Nicole I Stacy, David S Rotstein, Tom B Waltzek, Matt J Griffin, Ruth Francis-Floyd
This report describes a case of systemic bacterial infection caused by Edwardsiella tarda in a Western African lungfish (Protopterus annectens) exposed to poor environmental and husbandry conditions. The fish presented with a large, external ulcerative lesion and died 2 weeks after developing anorexia. Histological evaluation revealed multifocal areas of necrosis and heterophilic and histiocytic inflammation throughout multiple tissues. Gram stain identified small numbers of intra- and extracellular monomorphic Gram-negative 1 to 2 μm rod-shaped bacilli...
June 8, 2018: Journal of Fish Diseases
Hai Trong Nguyen, Thuy Thi Thu Nguyen, Yan-Chun Chen, Hung Vu-Khac, Pei-Chi Wang, Shih-Chu Chen
Vibriosis is a severe infection occurring in many commercially important marine fish species. In this study, vaccines containing Vibrio harveyi recombinant outer membrane protein K (rOmpK), outer membrane protein U (rOmpU) and rOmpK-OmpU fusion protein in addition to the metabolizable MontanideTM ISA 763 A VG adjuvant were developed and evaluated in the orange-spotted grouper. The results indicate that recombinant V. harveyi protein-based vaccines resulted in a remarkably higher expression of IL-1β and IL-8 at 24 hr, and greater antibody production, as early as 2 weeks postimmunization...
June 8, 2018: Journal of Fish Diseases
Roberto Bermúdez, Ana Paula Losada, Ana Manuela de Azevedo, Jorge Guerra-Varela, David Pérez-Fernández, Laura Sánchez, Francesc Padrós, Barbara Nowak, María Isabel Quiroga
Zebrafish has become a popular research model in the last years, and several diseases affecting zebrafish research facilities have been reported. However, only one case of naturally occurring viral infections was described for this species. In 2015, infectious spleen and kidney necrosis virus (ISKNV) was detected in zebrafish from a research facility in Spain. Affected fish showed lethargy, loss of appetite, abnormal swimming, distention of the coelomic cavity and, in the most severe cases, respiratory distress, pale gills and petechial haemorrhages at the base of fins...
June 7, 2018: Journal of Fish Diseases
Issei Nishiki, Takayuki Minami, Ayana Murakami, Truong Dinh Hoai, Atushi Fujiwara
Since 2011, high mortality rates and symptoms consistent with vibriosis have been observed in farmed amberjack (Seriola dumerili) in Japan. To identify 41 strains isolated from diseased amberjack, a multilocus sequence analysis using nine concatenated genes (ftsZ, gapA, gyrB, mreB, pyrH, recA, rpoA, topA and 16S rRNA) was conducted. Twenty-seven strains were identified as Vibrio harveyi, suggesting an epidemic of V. harveyi infection in amberjack farms. Other strains were identified as Vibrio anguillarum, Vibrio owensii and Photobacterium damselae subsp...
June 7, 2018: Journal of Fish Diseases
Haitham H Mohammed, Eric Peatman
Unusual persistent natural mortality occurred in a floating in-pond raceway system intensively stocked with channel and hybrid catfish beginning in early November 2016 up until March 2017. The temperature during the period of outbreak ranged from 7.2 to 23.7°C. Gross examination of freshly dead and moribund fish revealed pale gills, slight abdominal distension and swollen inflamed vents. Comprehensive necropsy of 20 fish demonstrated vast amounts of bloody ascitic fluid in the coelomic cavity, visceral congestion, splenomegaly and pale friable livers but macroscopically normal kidneys, suggesting systemic bacterial infection...
June 7, 2018: Journal of Fish Diseases
P K Sahoo, S Pattanayak, A Paul, M K Sahoo, P Rajesh Kumar, D Panda, B R Pillai
No abstract text is available yet for this article.
June 7, 2018: Journal of Fish Diseases
H Wünnemann, U Eskens, E Prenger-Berninghoff, C Ewers, M Lierz
Since the 1940s, the anadromous allis shad, Alosa alosa (L.), has suffered population declines throughout its distribution range in Europe. In context of EU-LIFE projects for the reintroduction of the allis shad in the Rhine system, a comprehensive study was started in 2012 to investigate infectious diseases occurring in allis shad. In course of the study, 217 mature and young-of-the-year allis shad originating from the wild population from the Gironde-Garonne-Dordogne system (GGD-system) and the Rhine system as well as 38 allis shad from the breeding population were examined by use of bacteriological and histological methods...
May 28, 2018: Journal of Fish Diseases
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