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Plant Cell Reports

Qing Fang, Qiong Wang, Hui Mao, Jing Xu, Ying Wang, Hao Hu, Shuai He, Junchu Tu, Chao Cheng, Guozheng Tian, Xianqiang Wang, Xiaopeng Liu, Chi Zhang, Keming Luo
AtDIV2 integrates ABA signaling to negatively regulate salt stress in Arabidopsis. AmDIV (DIVARICATA) is a functional MYB transcription factor (TF) that regulates ventral identity during floral development in Antirrhinum. There are six members of DIV homologs in Arabidopsis; however, the functions of these proteins are largely unknown. Here, we characterized an R-R-type MYB TF AtDIV2, which is involved in salt stress responses and abscisic acid (ABA) signaling. Although universally expressed in tissues, the nuclear-localized AtDIV2 appeared not to be involved in seedling development processes...
July 16, 2018: Plant Cell Reports
Ayumi Hirohata, Izumi Sato, Kimihiko Kaino, Yuji Iwata, Nozomu Koizumi, Kei-Ichiro Mishiba
Co-transformation of multiple T-DNA in a binary vector enabled CRISPR/Cas9-mediated HR in tobacco. HR occurred in a limited region around the gRNA target site. In this study, CRISPR/Cas9-mediated homologous recombination (HR) in tobacco (Nicotiana tabacum L. 'SR-1') was achieved using binary vectors comprising two (T1-T2) or three (T1-T2-T3) independent T-DNA regions. For HR donor with the tobacco acetolactate synthase gene, SuRB, T-DNA1 contained ΔSuRBW568L , which lacked the N-terminus region of SuRB and was created by three nucleotide substitutions (ATG to GCT; W568L), leading to herbicide chlorsulfuron (Cs) resistance, flanked by the hygromycin (Hm)-resistant gene...
July 13, 2018: Plant Cell Reports
Gang Song, Shaoxun Yuan, Xuehui Wen, Zheni Xie, Laiqing Lou, Bingyu Hu, Qingsheng Cai, Bin Xu
Transcriptome analysis of Cd-treated switchgrass roots not only revealed novel switchgrass transcripts and gene structures but also highlighted the indispensable role of HSF/HSP network in switchgrass Cd tolerance. Switchgrass (Panicum virgatum L.), a C4 perennial tall grass, can be used for revegetation of Cd-contaminated soil. In the present study, a comparative transcriptome analysis of Cd-treated switchgrass roots was conducted. The result revealed a total of 462 novel transcripts and refined gene structures of 2337 transcripts...
July 12, 2018: Plant Cell Reports
Magdalena Biedroń, Alicja Banasiak
The vascular system develops in response to auxin flow as continuous strands of conducting tissues arranged in regular spatial patterns. However, a mechanism governing their regular and repetitive formation remains to be fully elucidated. A model system for studying the vascular pattern formation is the process of leaf vascularization in Arabidopsis. In this paper, we present current knowledge of important factors and their interactions in this process. Additionally, we propose the sequence of events leading to the emergence of continuous vascular strands and point to significant problems that need to be resolved in the future to gain a better understanding of the regulation of the vascular pattern development...
July 10, 2018: Plant Cell Reports
Yeqiang Zheng, Zhiwen Liu, Yuhe Sun, Guanshan Liu, Aiguo Yang, Fengxia Li
Six unique ORFs were characterized in tobacco plants with sua-CMS sterile cytoplasm, identifying the mtDNA basis for pollen sterility. sua-CMS (cytoplasmic male sterility), the most widely used sterile system in tobacco hybrids, is the only CMS type identified as having no negative effects on agronomic or quality traits in tobacco (Nicotiana tabacum) and as being fully male sterile. CMS is often associated with alterations of mitochondrial DNA (mtDNA), including novel chimeric open reading frames (ORFs), which result from rearrangement and recombination...
June 29, 2018: Plant Cell Reports
Camilla Badiali, Giulia De Angelis, Giovanna Simonetti, Elisa Brasili, Eric de Castro Tobaruela, Eduardo Purgatto, Heng Yin, Alessio Valletta, Gabriella Pasqua
Water-soluble chitosan oligosaccharides (COS) affect xanthone and volatile organic compound content, as well as antifungal activity against human pathogenic fungi of extracts obtained from Hypericum perforatum root cultures. Several studies have demonstrated the elicitor power of chitosan on xanthone biosynthesis in root cultures of H. perforatum. One of the major limitations to the use of chitosan, both for basic and applied research, is the need to use acidified water for solubilization. To overcome this problem, the elicitor effect of water-soluble COS on the biosynthesis of both xanthones and volatile organic compounds (VOCs) was evaluated in the present study...
June 28, 2018: Plant Cell Reports
Kun Qiao, Liang Gong, Yanbao Tian, Hong Wang, Tuanyao Chai
Cysteine in the N-terminal metal-binding domain (N-MBD) of TaHMA2 participates in Zn2+ /Cd2+ binding and translocation in Arabidopsis. Wheat heavy metal ATPase 2 (TaHMA2) can transport Zn2+ and Cd2+ across membranes. A previous study showed that cysteine (Cys) and glutamate residues in the N-terminal metal-binding domain (N-MBD) were necessary for metal-binding and translocation of TaHMA2 in yeast. However, the function of TaHMA2 in plants was not fully revealed. In this study, we investigated the roles of the CCxxE and CPC motifs in the N-MBD and the N/C-terminal regions of TaHMA2 in Zn2+ /Cd2+ translocation in root and shoot of Arabidopsis...
June 23, 2018: Plant Cell Reports
Bin Huang, Jin-Mei Zhang, Xiao-Ling Chen, Xia Xin, Guang-Kun Yin, Juan-Juan He, Xin-Xiong Lu, Yuan-Chang Zhou
Cu/Zn SOD and other genes may be critical indicators of a stress response to reactive oxygen species (ROS) accumulation in 48 h germinated rice embryos subjected to vitrification cryopreservation. In the current study, reactive oxygen species (ROS) accumulation was investigated in 48 h germinated rice embryos during the vitrification-cryopreservation process. We found that vitrification-cryopreservation significantly affected ROS levels, especially superoxide anion levels, in 48 h germinated rice embryos...
June 20, 2018: Plant Cell Reports
Jun Zhou, Donald A Hunter, David H Lewis, Michael T McManus, Huaibi Zhang
Viral-induced gene silencing of selected biosynthetic genes decreased overall carotenoid accumulation in California poppy. Regulation of carotenogenesis was linked with pigment sequestration, not changes in biosynthetic gene expression. Genes of carotenogenesis are well described, but understanding how they affect carotenoid accumulation has proven difficult because of plant lethality when the pigments are lacking. Here, we used a Tobacco Rattle Virus-based virus-induced-gene-silencing (VIGS) approach in California poppy (Eschscholzia californica) to investigate how silencing of the carotenoid biosynthetic pathway genes affects carotenoid metabolite accumulation and RNA transcript abundance of the carotenoid biosynthetic pathway genes...
June 19, 2018: Plant Cell Reports
Fabíola OCampo Quintero, Layra G Pinto, Carla F Barsalobres-Cavallari, Mariana de Lara Campos Arcuri, Lilian Ellen Pino, Lázaro Eustáquio Pereira Peres, Mirian P Maluf, Ivan G Maia
A seed maturation protein gene (CaSMP) from Coffea arabica is expressed in the endosperm of yellow/green fruits. The CaSMP promoter drives reporter expression in the seeds of immature tomato fruits. In this report, an expressed sequence tag-based approach was used to identify a seed-specific candidate gene for promoter isolation in Coffea arabica. The tissue-specific expression of the cognate gene (CaSMP), which encodes a yet uncharacterized coffee seed maturation protein, was validated by RT-qPCR. Additional expression analysis during coffee fruit development revealed higher levels of CaSMP transcript accumulation in the yellow/green phenological stage...
June 13, 2018: Plant Cell Reports
Elżbieta Różańska, Weronika Czarnocka, Łukasz Baranowski, Jakub Mielecki, Janice de Almeida Engler, Mirosław Sobczak
After initial up-regulation, expression of TUBG1 and TUBG2 is significantly down-regulated in mature syncytia, but lack of expression of either of γ-tubulin genes reduces numbers of nematode infections and developing females. Infective second stage juveniles of sedentary plant parasitic nematode Heterodera schachtii invade the root vascular tissue and induce a feeding site, named syncytium, formed as a result of cell hypertrophy and partial cell wall dissolution leading to a multinucleate state. Syncytium formation and maintenance involves a molecular interplay between the plant host and the developing juveniles leading to rearrangements and fragmentation of the plant cytoskeleton...
June 12, 2018: Plant Cell Reports
Zhipeng Liu, Xiaoying Wang, Xi Chen, Guiqing Shi, Qianqian Bai, Kai Xiao
Wheat miRNA member TaMIR1139 targets genes functional in various families and plays crucial roles in regulating plant Pi starvation tolerance. Through regulating target genes at posttranscriptional or translational level, plant miRNAs are involved in mediating diverse biological processes associated with growth, development, and responses to adverse stresses. In this study, we characterized the expression pattern and function of TaMIR1139, a miRNA member of wheat (T. aestivum) under Pi deprivation. TaMIR1139 precursor is also present in N...
June 9, 2018: Plant Cell Reports
Boya Wang, Chuzhe Li, Xiangge Kong, Ying Li, Zhibin Liu, Jianmei Wang, Xufeng Li, Yi Yang
The RING-type E3 ligase AtARRE participates in the plant ABA responding as a negative regulator. Ubiquitination protease system (UPS) is significant in post-transcriptional regulation. In UPS, E3 ligase recognizes the substrate protein and mediates the polyubiquitin chain onto the substrate. Here, we identified a new gene, named Arabidopsis thaliana ABA-related RING-type E3 ligase (AtARRE), which induced by ABA and NaCl. AtARRE encodes a functional RING-type E3 ligase protein localized in nucleus and plasma membrane of Arabidopsis...
June 8, 2018: Plant Cell Reports
Meghna R Malik, Jihong Tang, Nirmala Sharma, Claire Burkitt, Yuanyuan Ji, Marie Mykytyshyn, Karen Bohmert-Tatarev, Oliver Peoples, Kristi D Snell
The rapid assessment of metabolic engineering strategies in plants is aided by crops that provide simple, high throughput transformation systems, a sequenced genome, and the ability to evaluate the resulting plants in field trials. Camelina sativa provides all of these attributes in a robust oilseed platform. The ability to perform field evaluation of Camelina is a useful, and in some studies essential benefit that allows researchers to evaluate how traits perform outside the strictly controlled conditions of a greenhouse...
June 7, 2018: Plant Cell Reports
Motselisi J Koetle, Dyfed Lloyd Evans, Varnika Singh, Sandy J Snyman, R Stuart Rutherford, M Paula Watt
Mutagenesis had no effect on number of stalks/plot, stalk height, fibre and sucrose content of mutants. Imazapyr tolerance is likely due to a S622N mutation in the acetolactate synthase gene. The herbicidal compound imazapyr is effective against weeds such as Cynodon and Rottboellia species that constrain sugarcane production. This study aimed to compare agronomic characteristics of three imazapyr tolerant mutants (Mut 1, Mut 6 and Mut 7) with the non-mutated N12 control after 18 months of growth, and to sequence the acetolactate synthase (ALS) gene to identify any point mutations conferring imazapyr tolerance...
June 5, 2018: Plant Cell Reports
Renu Pandey, Milan Kumar Lal, Krishnapriya Vengavasi
Hexaploid wheat is more responsive than tetraploid to the interactive effects of elevated [CO2 ] and low P in terms of carboxylate efflux, enzyme activity and gene expression (TaPT1 and TaPAP). Availability of mineral nutrients to plants under changing climate has become a serious challenge to food security and economic development. An understanding of how elevated [CO2 ] influences phosphorus (P) acquisition processes at the whole-plant level would be critical in selecting cultivars as well as to maintain optimum yield in limited-P conditions...
June 4, 2018: Plant Cell Reports
Jingxia Zhang, Furong Wang, Chuanyun Zhang, Junhao Zhang, Yu Chen, Guodong Liu, Yanxiu Zhao, Fushun Hao, Jun Zhang
A VIGS method by agroinoculation of cotton seeds was developed for gene silencing in young seedlings and roots, and applied in functional analysis of GhBI-1 in response to salt stress. Virus-induced gene silencing (VIGS) has been widely used to investigate the functions of genes expressed in mature leaves, but not yet in young seedlings or roots of cotton (Gossypium hirsutum L.). Here, we developed a simple and effective VIGS method for silencing genes in young cotton seedlings and roots by soaking naked seeds in Agrobacterium cultures carrying tobacco rattle virus (TRV)-VIGS vectors...
June 4, 2018: Plant Cell Reports
Zhiyuan Lv, Yan Huang, Bi Ma, Zhonghuai Xiang, Ningjia He
Two LysM-containing proteins, namely, MmLYP1 and MmLYK2, were identified in mulberry. These proteins might be involved in chitin signaling. The LysM1 of MmLYK2 is critical for their interactions. Chitin is a major component of fungal cell walls and acts as an elicitor in plant innate immunity. Lysin motif (LysM)-containing proteins are essential for chitin recognition. However, related studies have been rarely reported in woody plants. In this study, in mulberry, the expression of a LysM-containing protein, MmLYP1, was significantly up-regulated after treatment with chitin and pathogenic fungi...
May 30, 2018: Plant Cell Reports
Cong Guan, Yan-Hua Huang, Xin Cui, Si-Jia Liu, Yun-Zhuan Zhou, Yun-Wei Zhang
Genetic improvement through overexpressing PuP5CS in switchgrass is feasible for enhancing plant salt stress tolerance. Switchgrass (Panicum virgatum L.) has developed into a dedicated bioenergy crop. To improve the biomass production of switchgrass grown on different types of soil, abiotic stress tolerance traits are considered for its genetic improvement. Proline accumulation is a widespread response when plants are subjected to abiotic stresses such as drought, cold and salinity. In plants, P5CS gene encodes the key regulatory enzyme that plays a crucial role in proline biosynthesis...
May 25, 2018: Plant Cell Reports
Shouwei Tian, Linjian Jiang, Xiaxia Cui, Jie Zhang, Shaogui Guo, Maoying Li, Haiying Zhang, Yi Ren, Guoyi Gong, Mei Zong, Fan Liu, Qijun Chen, Yong Xu
No abstract text is available yet for this article.
May 24, 2018: Plant Cell Reports
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