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Veterinary Clinical Pathology

Ricardo Marcos, Ana Canadas, Liliana Leite-Martins, Jorge Ribeiro, Joana Santos, Augusto de Matos, John W Harvey, Marta Santos
No abstract text is available yet for this article.
February 22, 2018: Veterinary Clinical Pathology
Ernst Leidinger, Gregor Springler, Erika Furman, Anton Wallner
No abstract text is available yet for this article.
February 14, 2018: Veterinary Clinical Pathology
Samantha J M Evans, Chad B Frank, Paul R Avery, A Russell Moore
No abstract text is available yet for this article.
February 14, 2018: Veterinary Clinical Pathology
Jessica S Fortin, Jeremy Shomper, Fred Williams, Angela B Royal
No abstract text is available yet for this article.
February 13, 2018: Veterinary Clinical Pathology
Harold W Tvedten
No abstract text is available yet for this article.
February 11, 2018: Veterinary Clinical Pathology
Sunita Seshia, M Casey Gaunt, Beverly A Kidney, Marion L Jackson
BACKGROUND: Evidence suggests that administration of intravenous fluids impairs hemostasis. Thromboelastometry (TEM) may provide a more sensitive measure of the fluid effects on hemostasis than traditional coagulation tests. OBJECTIVE: The study aim was to determine if resuscitative fluid therapy affects hemostasis, as measured by TEM. MATERIALS AND METHODS: Using a randomized crossover design, 6 healthy dogs were administered intravenous colloid, crystalloid, and hypertonic saline at therapeutic doses...
February 11, 2018: Veterinary Clinical Pathology
Mary B Nabity, Kendal E Harr, Melinda S Camus, Bente Flatland, Linda M Vap
The purpose of this document is to provide total allowable error (TE a ) recommendations for commonly analyzed hematology measurands for veterinary personnel. These guidelines define relevant terminology and highlight considerations specific to hematology measurands. They also provide reasons and guidelines for using TE a in instrument performance evaluation, including recommendations for when the total observed error exceeds the recommended TE a . Biological variation-based quality specifications are briefly discussed...
February 11, 2018: Veterinary Clinical Pathology
Rafał Sapierzyński
A 15-year-old spayed female domestic short-haired cat with cutaneous/subcutaneous well-circumscribed, alopecic mass approximately 25 × 30 mm in diameter, localized to the left shoulder region was brought to the veterinary surgery department. Despite the suggestive location and macroscopic appearance, feline injection-site sarcoma was not suspected based on the cytologic examination of fine-needle aspirates. The tumor was surgically resected, and tissue sections were evaluated microscopically. The tumor was found to be nonencapsulated with a distinct border between the neoplastic parenchyma and surrounding connective tissue...
February 8, 2018: Veterinary Clinical Pathology
Samantha N Schlemmer, Erin M Scott, Lucien V Vallone, Mark C Johnson, Fengguang Guo, Guan Zhu
No abstract text is available yet for this article.
February 6, 2018: Veterinary Clinical Pathology
Labrini V Athanasiou, Panagiotis D Katsoulos, Eleni G Katsogiannou, Zoe S Polizopoulou, Myrto Diamantaki, Constantinos Kamatsos, Georgios Christodoulopoulos
BACKGROUND: Urine pH is an integral part of a complete urinalysis, and is commonly measured in veterinary practice using semiquantitative reagent strips. OBJECTIVE: The aim of this study was to compare the urine pH of dogs and sheep, using visual interpretation of dipstick reactions, and using a pH-meter as the reference method. Agreement between the 2 methods was also assessed. An additional objective was to compare the urine pH before and after centrifugation...
February 6, 2018: Veterinary Clinical Pathology
Luca Pazzini, Linda Mugnaini, Francesca Mancianti, Lorenzo Ressel
A 5-year-old indoor male neutered Siamese cat was presented with clinical signs of sneezing and chronic bilateral purulent nasal discharge. Multiple nasal cavity swabs were submitted for bacterial cultures, Mycoplasma felis-DNA qPCR, and cytology. M felisqPCR was positive and cytomorphologic diagnosis was severe, acute, purulent, rhinitis with intralesional protozoal microorganisms consistent with a Trichomonas spp. Nested PCR (nPCR) confirmed the diagnosis of Tritrichomonas foetus. Systemic therapy with doxycycline for M felis and metronidazole for T foetus was started with remission of clinical signs within 2 weeks; however, symptoms relapsed shortly after therapy was discontinued...
February 6, 2018: Veterinary Clinical Pathology
Itamar Aroch, Gad Baneth, Harold Salant, Yaarit Nachum-Biala, Asaf Berkowitz, Merav Shamir, Orit Chai
An 8-year-old mixed-breed dog was presented for acute, progressive weakness and ataxia, inappetence, and weight loss. The patient was mentally normal, but nonambulatory, with a right head tilt, right positional ventral strabismus, and slight head tremors. A neurologic lesion was localized to the cerebellum and right brainstem. Cerebrospinal fluid (CSF) analysis showed a markedly increased protein concentration and mixed pleocytosis, with eosinophil predominance (44%), intracytoplasmic inclusions within eosinophils, consistent with Ehrlichia canis (E canis) morulae, and Toxoplasma gondii (T gondii) or Neospora caninum (N caninum) tachyzoites within eosinophils and monocytes...
February 6, 2018: Veterinary Clinical Pathology
Paola Monti, Darren Barnes, Anna M Adrian, Roberta Rasotto
A 4-year-old Irish Setter was presented with a history of progressive left pelvic limb lameness. Orthopedic examination revealed pain on manipulation of the left stifle. Radiographs showed an osteolytic lesion in the subchondral bone of the medial tibial condyle. Fine-needle aspirates were taken, and cytology revealed numerous cohesive clusters of plump, oval to spindloid cells often with perivascular distribution and moderate cellular atypia. A diagnosis of sarcoma was made with synovial cell sarcoma (SCS) and histiocytic sarcoma being the 2 main differentials...
February 6, 2018: Veterinary Clinical Pathology
Martin G Randell, Nandhakumar Balakrishnan, Rebekah Gunn-Christie, Andrew Mackin, Edward B Breitschwerdt
Ineffective erythropoiesis was diagnosed in an 8-year-old male castrated Labrador Retriever. Despite treatment with immunosuppressive therapy for suspected immune-mediated erythrocyte maturation arrest, resolution of the nonregenerative anemia was not achieved. Following documentation of Bartonella henselae bacteremia by Bartonella alpha proteobacteria growth medium (BAPGM) enrichment blood culture, immunosuppressive therapy was discontinued, and the anemia resolved following prolonged antibiotic therapy. Bartonella immunofluorescent antibody testing was negative, whereas B henselae western blot was consistently positive...
February 2, 2018: Veterinary Clinical Pathology
Susanna Cirera, Line M Willumsen, Thea T Johansen, Lise N Nielsen
BACKGROUND: Gastrointestinal cancer accounts for approximately 8% of all canine malignancies. Early detection of cancer may have a tremendous impact on both treatment options and prognosis. MicroRNAs (miRNAs), a class of noncoding RNAs that can be found stably expressed in body fluids and feces, have been suggested as valuable human cancer biomarkers. OBJECTIVES: The purpose of the study was to investigate the feasibility of detecting miRNAs in canine feces and to determine the miRNA stability in fecal samples stored at different temperatures for different duration...
February 2, 2018: Veterinary Clinical Pathology
Matilde Piñeiro, Raquel Pato, Lourdes Soler, Raquel Peña, Natalia García, Carlos Torrente, Yolanda Saco, Fermín Lampreave, Anna Bassols, Francesca Canalias
BACKGROUND: In dogs, as in humans, C-reactive protein (CRP) is a major acute phase protein that is rapidly and prominently increased after exposure to inflammatory stimuli. CRP measurements are used in the diagnosis and monitoring of infectious and inflammatory diseases. OBJECTIVES: The study aim was to develop and validate a turbidimetric immunoassay for the quantification of canine CRP (cCRP), using canine-specific reagents and standards. METHODS: A particle-enhanced turbidimetric immunoassay was developed...
January 29, 2018: Veterinary Clinical Pathology
Romy M Heilmann, Niels Grützner, Stefanie Handl, Jan S Suchodolski, Jörg M Steiner
BACKGROUND: Calprotectin is a marker of inflammatory disorders in people, and serum and fecal calprotectin were shown to be increased in dogs with gastrointestinal inflammation. Biomarkers of gastrointestinal inflammation are currently lacking in cats. OBJECTIVES: The purpose of the study was to analytically validate the canine calprotectin radioimmunoassay for quantification of calprotectin in feline specimens. METHODS: The immunoassay was analytically validated by determining assay working range, dilutional parallelism, spiking recovery, and intra- and inter-assay variability...
January 29, 2018: Veterinary Clinical Pathology
Matteo Spano, Zuliani Davide, Peano Andrea, Bertazzolo Walter
A 3-year-old female mixed-breed dog was referred with a 2-day history of serious dyspnea, coughing, lethargy, anorexia, and a low-grade right anterior lameness. At presentation, the dog had an increased respiratory rate, dull heart and lung sounds, and cyanotic mucous membranes. It was hyperthermic and slightly dehydrated. Laboratory findings showed mild neutrophilia with a left shift, while serum biochemistry variables were in the normal range. However, urinalysis revealed mild proteinuria and rare erythrocytes and leukocytes on sediment examination...
January 25, 2018: Veterinary Clinical Pathology
Jesús Lescano, Miryam Quevedo, Marina Villalobos, Cesar M Gavidia
BACKGROUND: Hematologic and serum biochemical reference values obtained from captive or free-ranging wildlife populations may not be comparable as there can be significant variations due to preanalytic and analytic differences, including methods of capture and restraint, overall management in captivity including diet and composition of animal groups, and analytic methods being used. Hematology and serum biochemistry have never been studied in captive or free-ranging populations of Sechuran foxes (Lycalopex sechurae)...
January 24, 2018: Veterinary Clinical Pathology
Eva Sá E Lemos, Hugo Lima de Carvalho, Rui M Gil da Costa, Nazaré Pinto da Cunha
A 6-year-old female neutered European Shorthair cat was presented with a 2-day history of lethargy and hyporexia. On physical examination, the cat was slightly depressed and had a 2.5 cm nodule in the left 3rd mammary gland. The hemogram revealed mild leukocytosis with mature neutrophilia and moderate thrombocytopenia. On blood smear evaluation, rare pleomorphic cells, possibly of epithelial origin, were observed mainly at the feathered edge. The animal died about 12 hours after presentation, and a necropsy was performed...
January 23, 2018: Veterinary Clinical Pathology
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