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Veterinary Clinical Pathology

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https://www.readbyqxmd.com/read/30240029/aldosterone-producing-adrenocortical-carcinoma-with-myxoid-differentiation-in-a-cat
#1
Charalampos Attipa, Samuel Beck, Victoria Lipscomb, Kate English, Sofia Carvalho, Matti Kiupel, Balázs Szladovits, Laureen M Peters
A 10-year-old male neutered Persian cat was presented with an abdominal mass and history of weakness. Blood smear examination found marked elliptocytosis, and serum biochemical analysis revealed hypokalemia, hypochloremia, increased creatine kinase activity, and a high aldosterone concentration. Cytologic examination of the mass revealed neoplastic endocrine cells with moderate criteria of malignancy, favoring adrenocortical neoplasia. The adrenal mass was surgically excised and histologically characterized by lobules of mildly pleomorphic, polygonal neoplastic cells with moderate to abundant, occasionally granular, eosinophilic cytoplasm...
September 21, 2018: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/30229963/a-case-of-canine-high-grade-t-cell-lymphoma-immunophenotypically-consistent-with-t-zone-lymphoma
#2
Cyril Parachini-Winter, Kaitlin M Curran, Duncan S Russell, Elena Gorman
A 9-year-old spayed female Curly Coated Retriever was referred for evaluation of generalized peripheral lymphadenomegaly. The dog was clinically healthy on presentation with no anomalies detected on complete blood count, serum biochemistry, urinalysis, or three-view thoracic radiographs. Fine-needle aspiration (FNA) and cytology of the peripheral lymph nodes were consistent with lymphoma with an intermediate-sized lymphoid population. Flow cytometry of peripheral lymph nodes was consistent with a homogeneous population of CD4+ T cells that had lost expression of the pan-leukocyte antigen CD45...
September 19, 2018: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/30152856/analytical-quality-goals-a-review
#3
REVIEW
Bente Flatland, Melinda S Camus, Randolph M Baral
Analytical quality goals indicate how laboratory tests must perform to be clinically useful for their intended purpose. These goals have historically focused on analytical error assessment for quantitative methods and vary with measurand concentration or activity, and species. Although formalized quality goal models have been developed in human medicine, quality goals in veterinary medicine, to date, have not been formalized; use of human regulatory-based goals, consensus-based goals, or biologic variation-based goals have been reported most often...
August 28, 2018: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/30117540/synthetic-colloid-fluids-6-hydroxyethyl-starch-130-0-4-and-4-succinylated-gelatin-interfere-with-total-plasma-protein-measurements-in-vitro
#4
Elodie Yam, Giselle Hosgood, Gabriele Rossi, Lisa Smart
BACKGROUND: High molecular weight (MW) synthetic colloids interfere with refractometry. The effect of low MW synthetic colloids on refractometry is unknown. OBJECTIVES: To investigate the effect of 6% hydroxyethyl starch 130/0.4 (HES) and 4% succinylated gelatin (GELO) on the refractometric measurement of total plasma protein (TPP) and colloid osmotic pressure (COP) in canine plasma. METHODS: Heparinized plasma from 10 dogs was diluted with 0...
August 17, 2018: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/30088674/what-is-your-diagnosis-cystic-ovarian-structure-in-a-dog
#5
Niki Skeldon, Melanie Spoor, Jim Klaassen, Annalize Ide
No abstract text is available yet for this article.
August 8, 2018: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/30011078/what-is-your-diagnosis-bladder-mass-in-a-mare
#6
Susan Fielder, Melanie Breshears
No abstract text is available yet for this article.
July 16, 2018: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/30199124/reference-intervals-for-coagulation-variables-in-healthy-adult-domestic-goats-capra-aegagrus-hircus
#7
Kristin M Zersen, A Russell Moore, Christine S Olver, Justin C Mathis
BACKGROUND: Coagulation has not been studied extensively in adult goats, and reference intervals (RIs) for coagulation variables have not yet been established and would be clinically useful. OBJECTIVES: The goal of this study was to establish coagulation variable RIs in adult goats for future coagulation interpretation. METHODS: Blood samples from 40 healthy adult goats were collected, and assays for PT, APTT, D-dimers, antithrombin III, and fibrinogen were performed...
September 2018: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/30199123/research-and-diagnostic-tool-the-symmetric-dimethylarginine-case-for-research-use-only
#8
LETTER
Pasquale Santoro
No abstract text is available yet for this article.
September 2018: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/30199122/data-not-shown-times-have-changed-in-scientific-publishing-and-at-the-veterinary-clinical-pathology-journal
#9
EDITORIAL
Wendy Sprague
No abstract text is available yet for this article.
September 2018: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/30199121/analytical-validation-of-platelet-microparticle-quantification-in-cats
#10
Signe E Cremer, Jørgen Koch, Nanna Graversen, Anne S Gravgaard, Rebecca Langhorn, Annemarie T Kristensen, Jakob L Willesen, Lise N Nielsen
BACKGROUND: Cardiogenic embolism (CE) in cats is a devastating condition primarily associated with hypertrophic cardiomyopathy (HCM). Hypercoagulability may pose a risk for thrombus formation; however, no single test can predict CE development. Platelet microparticles (PMPs) released from platelet membranes are associated with thrombosis in humans. OBJECTIVES: The aims were to validate flow cytometric PMP quantification in cats analytically and, in a pilot study, evaluate the procoagulant annexin V (AnV) positive PMP concentration in healthy cats and cats with asymptomatic HCM...
September 2018: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/30168859/comparison-of-traditional-statistical-quality-control-using-commercially-available-control-materials-and-two-patient-based-quality-control-procedures-for-the-advia-120-hematology-system
#11
Lourdes C Vanyo, Kathleen P Freeman, Antonio Meléndez-Lazo, Mariana Teles, Rafaela Cuenca, Josep Pastor
BACKGROUND: Quality control procedures are an important part of the overall quality assurance for production of accurate and reliable hematologic results. OBJECTIVES: This study aimed to validate a quality control material-based procedure and assess two patient-based quality control procedures (repeat patient testing [RPT] and average of normals [AoN]) with the ADVIA 120 Hematology System. METHODS: Requirements for quality control procedures were obtained with the computerized statistical and quality program, EZRules3...
September 2018: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/30134504/evaluation-of-the-analytical-variability-of-urine-protein-to-creatinine-ratio-in-cats
#12
Marco Giraldi, Gabriele Rossi, Walter Bertazzolo, Stefano Negri, Saverio Paltrinieri, Paola Scarpa
BACKGROUND: Proteinuria quantification with the urinary protein-to-creatinine (UPC) ratio is part of the diagnostic process in feline patients suspected of chronic kidney disease (CKD). In affected cats, monitoring and substaging of the UPC according to the International Renal Interest Society (IRIS) guidelines is also necessary for appropriate patient management. No information is available about the possible effects of analytical variability on urinary proteins (UPs) and the UPC ratio in cats...
September 2018: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/30125968/correlation-of-electrophoretic-urine-protein-banding-patterns-with-severity-of-renal-damage-in-dogs-with-proteinuric-chronic-kidney-disease
#13
Jessica A Hokamp, Sidney A Leidy, Irina Gaynanova, Rachel E Cianciolo, Mary B Nabity
BACKGROUND: Urine protein loss is common in dogs with chronic kidney disease (CKD). Currently available noninvasive means of evaluating CKD in dogs cannot accurately predict the severity of glomerular and tubulointerstitial damage. Electrophoretic analysis of urine proteins can indicate the compromised renal compartment (glomerular vs tubular), but extensive evaluation of protein banding pattern associations with histologic damage severity has not been performed in dogs. OBJECTIVES: We aimed to evaluate electrophoretic banding patterns as indicators of the presence and severity of glomerular and tubulointerstitial damage in dogs with naturally occurring, predominantly proteinuric CKD...
September 2018: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/30117564/the-current-status-of-avian-aspergillosis-diagnoses-veterinary-practice-to-novel-research-avenues
#14
REVIEW
Masha G Savelieff, Lucia Pappalardo, Panagiotis Azmanis
Aspergillus fungal infections continue to be a significant cause of morbidity and mortality in birds that can, in part, be attributed to the lack of a diagnostic "gold standard" for Aspergillus infection, and which delays the diagnosis, treatment, and outcome of avian patients. At present, none of the available methods in veterinary care can detect aspergillosis early enough and with the accuracy, precision, and specificity required of an ideal diagnostic tool. Therefore, researching methods of Aspergillus detection is still an active area of inquiry, and novel techniques continue to emerge...
September 2018: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/30106484/phantastic-phagocytes
#15
Kathrin F Burke, Nicole I Stacy
No abstract text is available yet for this article.
September 2018: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/30102411/establishment-of-reference-intervals-for-serum-symmetric-dimethylarginine-in-adult-nonracing-greyhounds
#16
Rebekah Liffman, Thurid Johnstone, Brett Tennent-Brown, Graham Hepworth, Natalie Courtman
BACKGROUND: The reference intervals (RIs) for the renal biomarkers urea and creatinine, in Greyhounds, are higher than those for non-sighthound breeds. A recent study has demonstrated a higher concentration of another biomarker of renal function, symmetric dimethylarginine (SDMA), in Greyhounds compared with other dog breeds, and thus a breed-specific RI for serum SDMA may be appropriate for Greyhounds. Greyhounds appear to be predisposed to renal disease, and the establishment of an appropriate RI for SDMA may improve the ability to identify early renal dysfunction in this breed...
September 2018: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/30088675/unraveling-avian-and-reptilian-hematology-an-optical-and-electron-microscopic-study-of-the-buffy-coat
#17
Filipe Fontes Pinto, Célia Lopes, Fernanda Malhão, Ricardo Marcos
BACKGROUND: Blood centrifugation and buffy coats are at the cornerstone of hematology. In mammals, the buffy coat has a layered disposition (from bottom to top) with neutrophils on top of erythrocytes, followed by monocytes/lymphocytes, and platelets. In nonmammals, this distribution is unknown. Recently, the cell tube block (CTB) technique was developed to study the buffy coat, but it was never applied to nonmammal buffy coats. OBJECTIVES: This study aimed to evaluate using the CTB technique to study reptilian and avian buffy coats and to propose its use for clinical applications...
September 2018: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/30024652/thrombocytosis-and-central-nervous-system-involvement-in-a-case-of-canine-acute-megakaryoblastic-leukemia
#18
Daphné Rochel, Jérôme Abadie, Cynthia Robveille, Bérengère Déqueant, Elie Dagher, Françoise Roux, Laetitia Jaillardon
This case report presents a 14-month-old female Poodle mix with acute megakaryoblastic leukemia based on a marked thrombocytosis, abnormal platelet morphology, circulating dwarf megakaryocytes, and blast cells in the blood. Bone marrow abnormalities included dysmegakaryopoiesis dygranulopoiesis, and an increased number of blast cells was observed in the blood. Extensive leukemic involvement was also found in the liver, spleen, lymph nodes, lungs, kidneys, and brain. The cytopathologic features of the abnormal circulating cells were highly suggestive of being megakaryocytic in origin, which was supported by negative myeloperoxidase staining and positive von Willebrand factor staining on immunocytochemistry (ICC)...
September 2018: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/30024644/assessment-of-the-red-blood-cell-proteome-in-a-dog-with-unexplained-hemolytic-anemia
#19
Victoria L Black, Kate Heesom, Fran Whittington, Sean Davis, Séverine Tasker, Sophie Adamantos, Andrew D Davidson
A 7-year-old female neutered Jack Russell Terrier was presented to Langford Vets, the University of Bristol, with a history of chronic intermittent lethargy. Investigations and clinical course were compatible with hereditary hemolysis due to a red blood cell membrane defect. Proteomics was used to explore protein alterations in the presence of a hypothesized red blood cell membrane protein deficiency. Proteomic analysis revealed downregulation of the band 3, and alpha- and beta-adducin proteins, and alterations in the red blood cell proteome consistent with previous reports of changes due to the presence of reticulocytosis and ongoing hemolysis...
September 2018: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/30011069/the-effects-of-iatrogenic-blood-contamination-on-total-nucleated-cell-counts-and-protein-concentrations-in-canine-cerebrospinal-fluid
#20
Amy L MacNeill, Barbara G Andre, Yenlie Zingale, Rebecca A Packer, Stephanie McGrath
BACKGROUND: Cerebrospinal fluid (CSF) might be altered by iatrogenic blood contamination, precluding accurate diagnostic interpretation. OBJECTIVES: Available formulas to correct for iatrogenic blood contamination are likely unreliable. Study objectives were to determine the effects of blood contamination on total nucleated cell counts (NCCs) and protein concentrations in canine CSF. METHODS: Two methods were followed to evaluate the effect of blood contamination on total NCC and protein concentrations in CSF...
September 2018: Veterinary Clinical Pathology
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