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Veterinary Clinical Pathology

Sarah C Roode, Heather R Shive, Willemijn Hoorntje, Jennifer Bernard, Devorah M Stowe, Roy R Pool, Carol B Grindem
A 20-month-old female spayed Staffordshire Terrier (22.3 kg) presented to the Orthopedic Surgery Service at North Carolina State University Veterinary Teaching Hospital for evaluation of a 6-week history of toe-touching to nonweight-bearing lameness in the right hind limb. Radiographs of the right stifle revealed a multiloculated lytic lesion of the distal femur, with a large open lytic zone centrally, numerous osseous septations peripherally, and focal areas of cortical thinning and loss. An aspirate of the right distal femoral lesion yielded mildly cloudy serosanguineous fluid...
May 21, 2018: Veterinary Clinical Pathology
Ashleigh W Newman, Midori G Asakawa, Tracy Stokol
No abstract text is available yet for this article.
May 14, 2018: Veterinary Clinical Pathology
Graham Stock, Nikola Pantchev, Majda Globokar
No abstract text is available yet for this article.
May 9, 2018: Veterinary Clinical Pathology
Austin K Viall, Alejandro Larios Mora, Matthew T Brewer, Joseph S Smith, Amanda J Kreuder, Ryan M Breuer, Amanda J Fales-Williams
No abstract text is available yet for this article.
May 7, 2018: Veterinary Clinical Pathology
Angela B Royal, Brian Jochems
No abstract text is available yet for this article.
May 7, 2018: Veterinary Clinical Pathology
Maggie R McCourt, Alexandra M Dieterly, Paige E Mackey, Shane D Lyon, Theresa E Rizzi, Jerry W Ritchey
An 8-year-old, intact female, mixed-breed dog presented to the Oklahoma State University Boren Veterinary Medical Teaching Hospital for evaluation of progressive lameness and joint effusion of multiple joints. Physical examination revealed joint effusion of the elbow, hock, and stifle joints bilaterally, enlarged left axillary and right popliteal lymph nodes, a subcutaneous mass over the left elbow, and a subcutaneous mass involving the left second and third mammary glands. Cytologic examination of the mammary mass, enlarged lymph nodes, and joint fluid from most affected joints revealed a monomorphic population of loosely cohesive neoplastic epithelial cells...
May 7, 2018: Veterinary Clinical Pathology
Amir Zakian, Mohammad Nouri, Aria Rasooli, Masoud Ghorbanpour, Peter D Constable, Majid Mohammad-Sadegh
BACKGROUND: Inadequate absorption of colostral IgG1 is termed failure of transfer of passive immunity (FTPI). Dairy calves with FTPI have increased mortality and morbidity in their first 6 months of life. OBJECTIVES: This study compared the clinical performance of 5 methods for diagnosing FTPI in Holstein calves. METHODS: An observational study was performed using 160 Holstein heifer calves. Serum was harvested at 48 hours of age, and FTPI was assessed using a digital Brix refractometer for total solids measurements, and digital refractometry and the biuret method to measure serum total protein (STP) concentrations...
March 31, 2018: Veterinary Clinical Pathology
Signe E Cremer, Anne K H Krogh, Matilda E K Hedström, Liselotte B Christiansen, Inge Tarnow, Annemarie T Kristensen
BACKGROUND: Platelet microparticles (PMPs) are subcellular procoagulant vesicles released upon platelet activation. In people with clinical diseases, alterations in PMP concentrations have been extensively investigated, but few canine studies exist. OBJECTIVES: This study aims to validate a canine flow cytometric protocol for PMP quantification and to assess the influence of calcium on PMP concentrations. METHODS: Microparticles (MP) were quantified in citrated whole blood (WB) and platelet-poor plasma (PPP) using flow cytometry...
March 30, 2018: Veterinary Clinical Pathology
Benoît Cuq, Shauna L Blois, R Darren Wood, Gabrielle Monteith, Anthony C Abrams-Ogg, Christian Bédard, Geoffrey A Wood
BACKGROUND: Thrombin plays a central role in hemostasis and thrombosis. Calibrated automated thrombography (CAT), a thrombin generation assay, may be a useful test for hemostatic disorders in dogs. OBJECTIVES: To describe CAT results in a group of healthy dogs, and assess preanalytical variables and biological variability. ANIMALS: Forty healthy dogs were enrolled. METHODS: Lag time (Lag), time to peak (ttpeak), peak thrombin generation (peak), and endogenous thrombin potential (ETP) were measured...
March 26, 2018: Veterinary Clinical Pathology
Marco Giraldi, Saverio Paltrinieri, Andrea Zatelli
BACKGROUND: The dipstick is a first-line and inexpensive test that can exclude the presence of proteinuria in dogs. However, no information is available about the analytical variability of canine urine dipstick analysis. OBJECTIVES: The aim of this study was to assess the analytical variability in 2 dipsticks and the inter-operator variability in dipstick interpretation. METHODS: Canine urine supernatants (n = 174) were analyzed with 2 commercially available dipsticks...
March 26, 2018: Veterinary Clinical Pathology
Shannon Jones Hostetter, Hilary Z Hu, Joseph S Haynes
No abstract text is available yet for this article.
March 26, 2018: Veterinary Clinical Pathology
Mariko Sawa, Akira Yabuki, Moeko Kohyama, Noriaki Miyoshi, Osamu Yamato
BACKGROUND: Immunocytochemistry (ICC) is utilized as an advanced technique in veterinary cytology. In tumor diagnosis, cytokeratin and vimentin are markers used to distinguish the origin of tumor cells. Standard enzyme-based ICC has limitations in clinical use; and therefore, more convenient and reliable methods are needed. OBJECTIVES: The purpose of this study was to develop a rapid multiple immunofluorescent (RMIF) detection method for dual cytokeratin and vimentin staining on cytology slides in dogs...
March 9, 2018: Veterinary Clinical Pathology
Liza Bau-Gaudreault, Sébastien Overvelde, Donald Martin
No abstract text is available yet for this article.
March 9, 2018: Veterinary Clinical Pathology
Ashley Barratclough, Ruth Francis Floyd, Roger L Reep, Ray L Ball, Bobbi J Conner
BACKGROUND: Thromboelastography (TEG) provides a comprehensive evaluation of blood clot formation. This test can be used to identify abnormalities in coagulation by assessing multiple aspects of the clotting cascade, including the speed of clot initiation and formation, clot strength, and ultimately fibrinolysis. Thromboembolic disease has been hypothesized to play a role in the pathophysiology of cold stress syndrome (CSS), an important cause of mortality in the threatened Florida manatee (Trichechus manatus latirostris)...
March 9, 2018: Veterinary Clinical Pathology
Gian Lorenzo D'Alterio, Chris Barichievy, William Macasero
BACKGROUND: The Arabian Sand Gazelle (Gazella subgutturosa marica, Thomas, 1897) has been extirpated throughout the Arabian Peninsula. Captive breeding and reintroduction for conservation purposes require veterinary support and the use of hematology RIs. OBJECTIVES: The purpose of the study was to establish hematological RIs for the Arabian Sand Gazelle. METHODS: Blood from 231 clinically healthy captive and 22 wild (reintroduced) Arabian Sand Gazelles were sampled...
March 7, 2018: Veterinary Clinical Pathology
Bente Flatland, Kathleen P Freeman
BACKGROUND: Repeat patient testing-based quality control (RPT-QC) is a potential method for veterinary laboratories (eg, that have a limited budget for quality commercial control material [QCM] or that wish to use material with a species-specific matrix). OBJECTIVES: To determine whether total error (TEa ), probability of error detection (Ped), and probability of false rejection (Pfr) similar to that achievable with QC materials can be controlled using RPT-QC METHODS: Control limits (WBC, RBC, HGB, HCT, MCV, and PLT) for the Advia 120 (n = 23) and scil Vet ABC (n = 22) were calculated using data from normal canine specimens from a routine caseload...
March 5, 2018: Veterinary Clinical Pathology
Jennifer H Kopanke, Annie V Chen, Jourdan E Brune, Amanda C Brenna, Stephanie A Thomovsky
BACKGROUND: Lactate dehydrogenase (LD) exists as 5 isoenzymes (LD-1 through LD-5) that are expressed throughout the body and can be detected in both serum and cerebrospinal fluid (CSF). LD and its isoenzymes have been relatively unstudied in veterinary medicine, although studies in human medicine have demonstrated that changes in total LD activity and atypical isoenzyme patterns can indicate disease processes, including neurologic abnormalities. OBJECTIVES: The purpose of this study was to establish RIs for LD and its isoenzymes in the serum and CSF of clinically healthy dogs...
March 5, 2018: Veterinary Clinical Pathology
Anne K Korn, Natali Bauer, Andreas Moritz, Georg Erhardt
BACKGROUND: Literature regarding the impact of age and breed size on clinical biochemical measurands in rabbits is scarce. OBJECTIVES: We aimed to establish clinical biochemical measurand RIs for rabbits bred and kept under standard conditions and to evaluate the impact of gender, age, and breed size on the results using a Nova CRT8 analyzer (Nova Biomedical GmbH) and an ABX Pentra 400 analyzer (ABX Horiba, Axonlab). METHODS: Serum samples were available from 122 adult rabbits (56 males, 66 females) of 10 different breeds and crossbreds with a mean age of 264 ± 21 days...
March 5, 2018: Veterinary Clinical Pathology
Lisa J Schlein, Kathryn L Wycislo
No abstract text is available yet for this article.
March 2018: Veterinary Clinical Pathology
Ioannis L Oikonomidis, Christos Brozos, Evangelos Kiossis, Maria Kritsepi-Konstantinou
BACKGROUND: Clinical pathology investigations are essential to evaluate the health status and practice management of ruminants. Appropriate and accurate RIs are required for the interpretation of laboratory results. Despite substantial phenotypic differences and management-related disparities between male and female sheep, RIs in rams are sparse and limited by small reference populations and outdated methods. OBJECTIVES: The purpose of this study was to establish RIs for hematologic, biochemical, and hormonal analytes in adult rams using controlled preanalytic and analytic procedures, and to investigate whether breed-dependent partitioning of RIs is required...
March 2018: Veterinary Clinical Pathology
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