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Veterinary Clinical Pathology

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https://www.readbyqxmd.com/read/28085207/serum-creatine-kinase-isoenzymes-and-macroenzymes-in-dogs-with-different-neurologic-diseases
#1
Saverio Paltrinieri, Laura Pintore, Federica Balducci, Alessia Giordano, Annaluce Costabile, Marco Bernardini
BACKGROUND: Increased serum activity of CK isoenzymes and macroenzymes, and in particular of the brain isoenzyme (CK-BB) has been reported in dogs with central nervous system (CNS) disorders. However, no studies on the possible differences in serum activities of CK iso- or macroenzymes (Macro-CK1 and Macro-CK2) in different neurologic diseases are available. OBJECTIVE: The aim of this study was to describe the electrophoretic distribution of CK iso- and macroenzymes in dogs with CNS disorders in order to assess whether this distribution depends on a specific neurologic disease...
January 13, 2017: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/28067962/concurrent-multiple-myeloma-and-mast-cell-neoplasia-in-a-13-year-old-castrated-male-maine-coon-cat
#2
Jonathan M Bagwell, Heather R Herd, Melanie A Breshears, Steven Hodges, Theresa E Rizzi
A 13-year-old, castrated male Maine Coon cat was presented to Oklahoma State University Boren Veterinary Medical Teaching Hospital for yearly echocardiographic examination monitoring hypertrophic cardiomyopathy (HCM) diagnosed in 2003. Physical examination revealed a heart murmur and premature beats, likely related to HCM, but was otherwise unremarkable. A biochemistry profile revealed a hyperglobulinemia (6.3 g/dL). Cytologic examination of fine-needle aspirates from liver and spleen revealed increased numbers of plasma cells and mast cells, confirmed with subsequent histologic examination...
January 9, 2017: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/28026883/what-is-your-diagnosis-a-mass-on-the-right-tarsus-in-a-rabbit
#3
Lilani Munasinghe, Christina Dougherty, Santhi Sridharan, Helene Philibert, Beverly Kidney
No abstract text is available yet for this article.
December 27, 2016: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/28001311/extreme-lymphocytosis-with-myelomonocytic-morphology-in-a-horse-with-diffuse-large-b-cell-lymphoma
#4
Kristina Meichner, Blaire H Kraszeski, Jessica R Durrant, Carol B Grindem, Babetta A Breuhaus, Peter F Moore, Jennifer A Neel, Keith E Linder, Luke B Borst, Jonathan E Fogle, Jaime L Tarigo
An 11-year-old, 443-kg Haflinger mare was presented to the North Carolina State University Veterinary Teaching Hospital with a 2-week history of lethargy and a 3-day duration of anorexia, pyrexia, tachycardia, and ventral edema. Severe pitting edema, peripheral lymphadenopathy, and a caudal abdominal mass were noted on physical examination. An extreme leukocytosis (154.3 × 10(3) /μL) and microscopic hematologic findings suggestive of myelomonocytic leukemia were observed. Serum protein electrophoresis revealed a monoclonal gammopathy and urine protein electrophoresis revealed a monoclonal light chain proteinuria...
December 21, 2016: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/27935633/nodular-presentation-of-dirofilaria-repens-infection-in-a-cat-mimicking-a-fibrosarcoma
#5
Simone Manzocchi, Matthias Lendner, Eleonora Piseddu, Valeria Sebastiani, Simona Morabito, Arwid Daugschies, Nikola Pantchev
A cat with multiple subcutaneous nodules suggesting a soft tissue sarcoma by physical and computed tomographic examination was diagnosed as being affected by subcutaneous filariosis based on cytologic and ultrasonographic assessments. Nodules were surgically removed and extracted nematodes were identified by PCR as Dirofilaria repens. Furthermore, DNA of Dipetalonema dracunculoides (syn. Acantocheilonema dracunculoides) was detected by PCR, with no evidence of circulating microfilariae. To the best of the authors' knowledge, this represents the first report describing adults of D repens in multiple subcutaneous nodules in a cat...
December 9, 2016: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/27935625/comparison-of-established-and-novel-methods-for-the-detection-and-enumeration-of-microparticles-in-canine-stored-erythrocyte-concentrates-for-transfusion
#6
Meredeth C McEntire, K Jane Wardrop, William C Davis
BACKGROUND: Microparticles (MP) are submicron, phosphatidylserine (PS)-bearing lipid vesicles with physiologic and pathologic roles in coagulation and inflammation. Microparticles accumulate in packed RBC (pRBC) stored for transfusion, potentially increasing recipient morbidity. Historically, canine MP have been detected with the PS label annexin V in supernatant samples. Other detection methods are available but have not been evaluated in dogs. OBJECTIVES: The purpose of the study was to detect and enumerate MP in canine pRBC using annexin V, lactadherin, and bio-maleimide to compare label performance and assess microparticle accumulation under standard storage conditions...
December 9, 2016: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/27874969/idiopathic-myelofibrosis-accompanied-by-peritoneal-extramedullary-hematopoiesis-presenting-as-refractory-ascites-in-a-dog
#7
Yolandi Rautenbach, Amelia Goddard, Sarah J Clift
A 2.5-year-old spayed female American Pit Bull Terrier dog presented with a primary complaint of chronic refractory ascites. The dog's CBC displayed a moderate to severe macrocytic, hypochromic, nonregenerative anemia, and a moderate leukopenia as result of a moderate neutropenia and monocytopenia. Microscopic examination of the blood smear showed marked anisocytosis, mild polychromasia, mild acanthocytosis and ovalocytosis, moderate schistocytosis and poikilocytosis, and 4 metarubricytes/100 WBC. Abdominal ultrasonography revealed a homogenous, mild to moderately hyperechoic appearing liver as well as marked amounts of speckled anechoic to slightly hypoechoic peritoneal fluid...
November 22, 2016: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/27870070/what-is-your-diagnosis-skin-scraping-from-a-hellbender
#8
Allison Dusick, Bente Flatland, Linden Craig, Sylvia Ferguson
No abstract text is available yet for this article.
November 21, 2016: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/27977062/comparison-of-hematology-plasma-biochemistry-and-blood-gas-variables-between-2-venipuncture-sites-in-southern-stingrays-dasyatis-americana
#9
Brianne E Phillips, Emily F Christiansen, Michael K Stoskopf, Heather Broadhurst, Robert George, Craig A Harms
BACKGROUND: The Southern Stingray (Dasyatis americana) is a batoid elasmobranch frequently exhibited in zoological institutions. Blood is commonly collected from the caudal hemal arch at the tail base in stingrays for the purpose of health assessment and clinical pathology tests. An alternative site that allows a dorsal or ventral approach without necessitating puncture of a cartilaginous structure has been identified between the cartilaginous pectoral fin rays (ceratotrichia). OBJECTIVES: The purpose of the study was to compare CBC, plasma biochemistry analytes, and blood gas variables between blood samples collected from the caudal and pectoral fin vasculature sites of the Southern Stingray...
December 2016: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/27977061/erratum
#10
(no author information available yet)
No abstract text is available yet for this article.
December 2016: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/27977060/erratum
#11
(no author information available yet)
No abstract text is available yet for this article.
December 2016: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/27977059/comparison-of-a-rapid-immunochromatographic-assay-with-an-immunofluorescent-antibody-test-for-detection-of-leishmania-infantum-antibodies-in-dogs
#12
Daniela Proverbio, Eva Spada, Roberta Perego, Luciana Baggiani, Giada Bagnagatti De Giorgi, Antonella Migliazzo, Fabrizio Vitale
BACKGROUND: Identification of Leishmania infantum-infected dogs is crucial for control of canine leishmaniosis. In particular, in areas where access to specialized laboratories is limited, the availability of reliable and rapid in-clinic serologic tests may support immediate diagnosis in suspected cases and permit detection of asymptomatic canine carriers of L infantum infection. OBJECTIVE: The purpose of the study was to validate the immunochromatographic test (ICT) Anigen Rapid Leishmania Ab Test kit for detection of L infantum antibodies in naturally exposed dogs in comparison with the immunofluorescence antibody test (IFAT)...
December 2016: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/27977058/can-evidence-based-medicine-be-applied-to-the-theory-of-reference-intervals
#13
EDITORIAL
Kristen R Friedrichs
No abstract text is available yet for this article.
December 2016: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/27935623/reducing-blood-volume-requirements-for-clinical-pathology-testing-in-toxicologic-studies-points-to-consider
#14
Florence Poitout-Belissent, Adam Aulbach, Niraj Tripathi, Lila Ramaiah
In preclinical safety assessment, blood volume requirements for various endpoints pose a major challenge. The goal of this working group was to review current practices for clinical pathology (CP) testing in preclinical toxicologic studies, and to discuss advantages and disadvantages of methods for reducing blood volume requirements. An industry-wide survey was conducted to gather information on CP instrumentation and blood collection practices for hematology, clinical biochemistry, and coagulation evaluation in laboratory animals involved in preclinical studies...
December 2016: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/27880026/the-cryptic-cryptococcus
#15
Ricardo Marcos, Fernanda Malhão, Joana Santos, Sara Marques, Gertrude Thompson, Marta Santos
No abstract text is available yet for this article.
December 2016: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/27870110/the-hematology-of-captive-bobtail-lizards-tiliqua-rugosa-blood-counts-light-microscopy-cytochemistry-and-ultrastructure
#16
Cheryl A Moller, Tibor Gaál, Jennifer N Mills
INTRODUCTION: Bobtail lizards (Tiliqua rugosa) are native to Australia. The only previous study on the hematology of this species documented just 6 animals. OBJECTIVES: The aims of this study were to characterize the light microscopy, ultrastructure and cytochemistry of blood cells, and evaluate CBCs of captive Bobtail lizards. METHODS: Over 2 consecutive summers, heparinized venous blood was collected from the ventral coccygeal vein of 46 clinically healthy, captive indoor- or outdoor-housed adult Bobtails...
December 2016: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/27870071/eosinophilic-meningomyelitis-associated-with-t-cell-lymphoma-in-a-cat
#17
Kathryn Y Bray, Karen R Muñana, Kristina Meichner, Laura A White, Gabriela Seiler
A 12-year-old cat was presented for evaluation of progressive tetraparesis. Magnetic resonance imaging of the cervical spine demonstrated T2-hyperintensity, and contrast enhancement within the C4-C7 spinal cord, with marked meningeal contrast enhancement and segmental nerve root thickening. Lumbar cerebrospinal fluid contained 407 total nucleated cells/μL, with 99% eosinophils. The cat transiently improved with prednisolone, clindamycin, and ivermectin therapy, but subsequently worsened and was euthanized...
December 2016: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/27870069/suspected-myelodysplastic-myeloproliferative-neoplasm-in-a-feline-leukemia-virus-negative-cat
#18
Amy L Weeden, Kyle R Taylor, Scott P Terrell, Alexander E Gallagher, Heather L Wamsley
A 10-year-old castrated Domestic Short-Haired cat was presented to a primary care veterinarian for a wellness examination and laboratory examination for monitoring of diabetes mellitus. The CBC revealed marked thrombocytosis, leukopenia and macrocytic, normochromic anemia. The cat tested negative for FeLV and feline immunodeficiency virus, but was positive for Mycoplasma haemominutum by PCR. Hematologic abnormalities were not responsive to therapy, so a repeat CBC and a bone marrow aspiration for cytology were performed...
December 2016: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/27859449/cytocentrifuge-preparation-in-veterinary-cytology-a-quick-simple-and-affordable-manual-method-to-concentrate-low-cellularity-fluids
#19
Ricardo Marcos, Marta Santos, Carla Marrinhas, Carla Correia-Gomes, Mario Caniatti
BACKGROUND: Smears prepared by cytocentrifugation, the so-called cytospins, are widely used in human and veterinary cytology. However, the high cost has hampered the availability of commercial cytospin centrifuges in some veterinary clinics and laboratories. Nevertheless, cytospins are important for evaluating fluids with very low cellularity such as cerebrospinal fluid (CSF) or bronchoalveolar lavage fluid (BALF). OBJECTIVES: The aim of this study was to devise and test the use of a low-cost, in-house manual cytocentrifuge to obtain cytospin preparations...
December 2016: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/27805734/what-is-your-diagnosis-pulmonary-mass-in-a-dog
#20
Nicole L Young, Ruth A Houseright, Cecilia S Robat
No abstract text is available yet for this article.
December 2016: Veterinary Clinical Pathology
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