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Veterinary Clinical Pathology

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https://www.readbyqxmd.com/read/28430367/effect-of-dalteparin-administration-on-thrombin-generation-kinetics-in-healthy-dogs
#1
Carolyn Gara-Boivin, Jérôme R E Del Castillo, Marilyn E Dunn, Christian Bédard
BACKGROUND: Dalteparin is used to prevent thrombotic complications in dogs. Measurement of anti-factor Xa (anti-FXa) activity is currently used for monitoring therapy, but remains a nonfunctional test. The calibrated automated thrombogram (CAT) could be a suitable approach for functional monitoring. OBJECTIVES: We hypothesized that the CAT will detect decreased endogenous thrombin potential (ETP) in healthy dogs receiving dalteparin. METHODS: Twenty-four healthy adult Beagles were randomly allocated to 4 equal groups...
April 21, 2017: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/28422312/what-is-your-diagnosis-cutaneous-ulcerated-nodule-in-a-geriatric-dog
#2
Marta Santos, Ana Canadas, Pablo Puente-Payo, Patrícia Dias-Pereira, Ricardo Marcos
No abstract text is available yet for this article.
April 19, 2017: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/28422307/what-is-your-diagnosis-fine-needle-aspirate-of-a-lung-nodule-and-bronchoalveolar-lavage-from-a-dog
#3
Jelena Palić, Kathrin Busch, Stefan Unterer
No abstract text is available yet for this article.
April 19, 2017: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/28419525/method-comparison-and-generation-of-plasma-biochemistry-ris-for-the-white-rhinoceros-on-a-point-of-care-and-wet-chemistry-analyzer
#4
Emma H Hooijberg, Gerhard Steenkamp, Peter Buss, Amelia Goddard
BACKGROUND: Plasma biochemistry analysis may aid the management of White rhinoceros with poaching or other injuries. Analyzer- and species-specific RIs are necessary to evaluate health status and identify abnormalities. OBJECTIVES: The purpose of the study was to perform a comparison study between the IDEXX VetTest and Roche Cobas Integra 400 Plus, and to generate analyzer-specific RIs for White rhinoceros according to published guidelines. METHODS: Comparison was carried out using White rhinoceros plasma samples from healthy and diseased animals...
April 17, 2017: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/28411369/comparison-of-equine-platelet-function-and-survival-in-whole-blood-collected-in-acid-citrate-dextrose-solution-or-citrate-phosphate-dextrose-adenine-solution
#5
Rana Bozorgmanesh, Julie W Sutton-Burges, Fern Tablin
BACKGROUND: Equine whole blood collection and storage methods have been evaluated to assess red blood cell viability; however, platelet (PLT) viability has not been comprehensively assessed. OBJECTIVES: The purpose of the study was to compare viability of PLTs collected in whole blood into 2 different anticoagulants. METHODS: Whole blood from 6 healthy adult Thoroughbred horses was collected into citrate-phosphate-dextrose-adenine (CPDA) or acid-citrate-dextrose (ACD)...
April 14, 2017: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/28403556/assessment-of-endogenous-25-hydroxyvitamin-d-serum-concentrations-by-liquid-chromatography-tandem-mass-spectrometry-in-various-animal-species
#6
Carol A Fritz, Kimberly A Navetta, David P Wolford, Jennifer L Colangelo
BACKGROUND: Mass spectrometry (MS) has become the preferential method for the analysis of vitamin D in the clinic, yet no single platform is utilized for preclinical species in drug development studies. For vitamin D, the MS platform can provide certain benefits such as applicability of a single assay for multiple species, low cost, and high specificity. OBJECTIVES: A quantitative liquid chromatography-tandem MS (LC-MS/MS) assay for 25-hydroxyvitamin D3 (25OHD3 ) and D2 (25OHD2 ) was validated for rat, dog, mouse, and monkey, and suitability for drug development studies was assessed...
April 12, 2017: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/28394428/what-is-your-diagnosis-inguinal-lymph-node-aspirate-from-a-dog
#7
Corry K Yeuroukis, Jessica M Thiman, Anne C Avery, Melinda S Camus, Jaime L Tarigo
No abstract text is available yet for this article.
April 10, 2017: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/28387422/validation-and-determination-of-a-reference-interval-for-canine-hba1c-using-an-immunoturbidimetric-assay
#8
Anne F Goemans, Susanna J Spence, Ian K Ramsey
BACKGROUND: Hemoglobin A1c (HbA1c) provides a reliable measure of glycemic control over 2-3 months in human diabetes mellitus. In dogs, presence of HbA1c has been demonstrated, but there are no validated commercial assays. OBJECTIVE: The purpose of the study was to validate a commercially available automated immunoturbidimetric assay for canine HbA1c and determine an RI in a hospital population. METHODS: The specificity of the assay was assessed by inducing glycosylation in vitro using isolated canine hemoglobin, repeatability by measuring canine samples 5 times in succession, long term inter-assay imprecision by measuring supplied control materials, stability using samples stored at 4°C over 5 days and -20°C over 8 weeks, linearity by mixing samples of known HbA1c in differing proportions, and the effect of anticoagulants with paired samples...
April 7, 2017: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/28370271/what-is-your-diagnosis-cystic-prostatic-lesion-in-a-domestic-ferret
#9
Liza Bau-Gaudreault, Carolyn N Grimes
No abstract text is available yet for this article.
April 3, 2017: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/28370193/what-is-your-diagnosis-multilobate-nasal-mass-in-a-5-month-old-sphynx-cat
#10
Veronika Simerdova, Milos Vavra, Misa Skoric, Ivo Hajek, Ondrej Skor
No abstract text is available yet for this article.
April 3, 2017: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/28370080/evaluation-of-assays-for-quantification-of-dna-in-canine-plasma-as-an-indirect-marker-of-netosis
#11
Stephanie A Smith, Corinne M Lawson, Maureen A McMichael, Katrina Jung, Mauria O'Brien, Ron Achiel
BACKGROUND: Neutrophil extracellular traps (NET), consisting of a filamentous DNA/chromatin-histone scaffold originating from neutrophils are part of the innate immune response, may be released under a variety of inflammatory conditions and are associated with an increased risk for thrombosis. OBJECTIVES: The purpose of this study was to evaluate a SYTOX green fluorescence assay and a histone-DNA complex (hisDNA) ELISA for quantification of NET-related DNA in canine plasma...
April 3, 2017: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/28370056/recommendations-for-designing-and-conducting-veterinary-clinical-pathology-biologic-variation-studies
#12
REVIEW
Kathleen P Freeman, Randolph M Baral, Navneet K Dhand, Søren Saxmose Nielsen, Asger L Jensen
The recent creation of a veterinary clinical pathology biologic variation website has highlighted the need to provide recommendations for future studies of biologic variation in animals in order to help standardize and improve the quality of published information and to facilitate review and selection of publications as standard references. The following recommendations are provided in the format and order commonly found in veterinary publications. A checklist is provided to aid in planning, implementing, and evaluating veterinary studies on biologic variation (Appendix S1)...
April 3, 2017: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/28370186/neoplastic-pleural-effusion-and-intrathoracic-metastasis-of-a-scapular-osteosarcoma-in-a-dog-a-multidisciplinary-integrated-diagnostic-approach
#13
Luis Mesquita, Jeremy Mortier, Lorenzo Ressel, Riccardo Finotello, Paolo Silvestrini, Martina Piviani
A 10-year-old, female spayed mixed-breed or cross-bred dog was referred to the Small Animal Teaching Hospital of the University of Liverpool due to tachypnea, dyspnea, and pleural effusion not responding to diuretics and antibiotics. The chest was drained and cytology of the pleural fluid was consistent with a modified transudate with presence of atypical cells initially attributed to mesothelial hyperplasia and dysplasia. Computed tomography detected, in addition to the bilateral pleural effusion, diffuse pleural thickening, multiple pleural and pulmonary nodules, and a mineralized and lytic mass in the left scapula...
March 29, 2017: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/28346702/evaluation-of-the-effects-of-time-temperature-and-specimen-storage-on-in-vitro-lactate-concentrations-in-healthy-dogs
#14
Colin F Reich, Jonathan F Bach, Julie M Walker
BACKGROUND: Lactate concentrations increase significantly under certain storage conditions, except for when glycolysis-arresting agents are used. Evaluation of time and storage conditions on heparinized whole blood lactate concentrations without glycolysis-arresting agents have not been evaluated in dogs. OBJECTIVES: The purpose of the study was to determine the effects of preanalytic storage conditions on the in vitro concentration of lactate in heparinized canine venous blood specimens...
March 27, 2017: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/28346682/age-related-variation-in-the-cellular-composition-of-equine-bronchoalveolar-lavage-fluid
#15
Shannon J Hostetter, Sandra K Clark, Jessica M Gilbertie, Sarah A Wiechert, Doug E Jones, Brett A Sponseller
BACKGROUND: Previous reports reveal variation in the cellular composition of equine bronchoalveolar lavage fluid (BALF). OBJECTIVES: The purpose of this study was to compare the profiles of BALF from horses to assess age-related differences. Serial BALF samples were collected from the same individuals over a one-year period to identify changes in individual animals as they aged. METHODS: Collection of BALF was performed on horses aged one week and one, 2, 6, and 12 months...
March 27, 2017: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/28295452/presumed-lupus-erythematosus-cells-identified-in-bronchoalveolar-lavage-fluid-from-a-mexican-hairless-dog
#16
Laura J Black, Ashley C Hechler, Maura E Duffy, Sarah S K Beatty
A neutered male Mexican Hairless dog was presented for generalized weight loss and weakness. Initial laboratory testing and diagnostic imaging revealed thrombocytopenia and an interstitial to miliary lung pattern affecting all lung fields. Mild joint effusion was found on physical examination affecting the stifle, tarsal, carpal, and elbow joints. Examination of synovial fluid demonstrated an inflammatory polyarthropathy in 3 joints. Cytocentrifuged and direct preparations of the bronchoalveolar lavage (BAL) fluid sample were made and cells consistent with lupus erythematosus (LE) cells and ragocytes were found...
March 13, 2017: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/28272815/clinically-healthy-overweight-and-obese-dogs-differ-from-lean-controls-in-select-cbc-and-serum-biochemistry-values
#17
Lauren B Radakovich, Matthew P Truelove, Stephen C Pannone, Christine S Olver, Kelly S Santangelo
BACKGROUND: Obesity is a global disease, affecting nearly half a billion people. Unfortunately, this trend is mirrored in our canine population. OBJECTIVES: As obesity is a complex inflammatory disease, there is a need to determine whether routine medical screening tests may indicate, or be influenced by, its presence. The objective of the current study was to determine if significant differences exist between CBC and biochemical profile values from control vs overweight/obese, client-owned dogs considered clinically healthy...
March 8, 2017: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/28263407/bartonella-henselae-in-canine-cavitary-effusions-prevalence-identification-and-clinical-associations
#18
Amy L Weeden, Natalie A Cherry, Edward B Breitschwerdt, Avery G Cheves, Heather L Wamsley
BACKGROUND: Previous reports suggest an association between Bartonella infection and effusions in dogs and human beings. OBJECTIVES: The aims of this study were to determine the prevalence of Bartonella infection in canine effusions and to investigate historic and clinical parameters predictive of Bartonella in dogs with effusions. METHODS: Canine cavitary effusions submitted for analysis and, if available, paired EDTA blood, were screened for Bartonella infection using the Bartonella α-proteobacteria growth medium enrichment culture/PCR diagnostic platform (Bartonella enrichment PCR or ePCR) at Galaxy Diagnostics, Inc...
March 6, 2017: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/28263401/what-is-your-diagnosis-joint-and-associated-tissue-aspirates-from-a-bearded-dragon-pogona-vitticeps
#19
Kate E Pennick, Rachael A Holicky, Melinda J Wilkerson
No abstract text is available yet for this article.
March 6, 2017: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/28249100/cytologic-features-of-cutaneous-follicular-tumors-and-cysts-in-dogs
#20
Adeyemi O Adedeji, Verena K Affolter, Mary M Christopher
BACKGROUND: Follicular tumors and cysts are common skin lesions in dogs. Both are distinguished based on their cellular origin (matrical, isthmus, or infundibular cells) and the type of keratin they produce. Typically, differentiation requires histopathology, as all these lesions often have similar cytologic features. OBJECTIVES: The goal of this retrospective study was to identify unique cytologic features that may assist differentiation of canine benign follicular tumors and cysts at cytology, using histopathology as gold standard...
March 1, 2017: Veterinary Clinical Pathology
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