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International Microbiology: the Official Journal of the Spanish Society for Microbiology

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https://www.readbyqxmd.com/read/27845499/distribution-of-virulence-genes-involved-in-biofilm-formation-in-multi-drug-resistant-acinetobacter-baumannii-clinical-isolates
#1
Rapee Thummeepak, Phattaraporn Kongthai, Udomluk Leungtongkam, Sutthirat Sitthisak
Acinetobacter baumannii is an opportunistic bacterial pathogen that is the major cause of hospital-acquired infections. It has been shown that A. baumannii with high biofilm formation increases the risk of acquiring infection. In this study, the prevalence of virulence genes involved in biofilm formation was determined in 225 A. baumannii clinical isolates from three hospitals in Thailand. Most of the isolates were multidrug-resistant A. baumannii strains (86.2%). Among all isolates, 76.9% (173/225) showed biofilm formation ability...
June 2016: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/27845498/spatial-homogeneity-of-bacterial-and-archaeal-communities-in-the-deep-eastern-mediterranean-sea-surface-sediments
#2
Sabine Keuter, Baruch Rinkevich
The diversity of microorganisms inhabiting the deep sea surface sediments was investigated in 9 stations (700-1900 m depth) in the Levantine basin by 454 massive tag sequencing of the 16S rDNA V4 region using universal primers. In total, 108,811 reads (an average of 10,088 per sample) were assigned to 5014 bacterial and 966 archaeal operational taxonomic units (OTUs; at 97% cut off). The 55% of the reads were of archaea, indicating dominance of archaea over bacteria at eight of the stations. The diversity and estimated richness values were high (e...
June 2016: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/27845497/lysozyme-as-a-cotreatment-during-antibiotics-use-against-vaginal-infections-an-in-vitro-study-on-gardnerella-vaginalis-biofilm-models
#3
Olivier Thellin, Willy Zorzi, Danièle Zorzi, Philippe Delvenne, Ernst Heinen, Benaïssa ElMoualij, Pascale Quatresooz
Bacterial vaginoses are frequent in women, most of them involving Gardnerella vaginalis. In more than 50% of the cases, usual antibiotic treatments are not capable of eliminating completely the infection, leading to recurrent vaginosis. In addition to the appearance of antibiotic resistance, recurrence can be due to the development of a biofilm by G. vaginalis. In vitro experiments on G. vaginalis biofilms showed that the biofilm protected bacteria from the antibiotic clindamycin. Also, recombinant human lysozyme (rhLys) was able to both degrade biofilms and prevent their formation...
June 2016: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/27845496/characterization-of-the-microbiota-associated-to-pecten-maximus-gonads-using-454-pyrosequencing
#4
Aide Lasa, Alex Mira, Anny Camelo-Castillo, Pedro Belda-Ferre, Jesús L Romalde
A next-generation sequencing (NGS) approach was used to study the microbiota associated to Pecten maximus broodstock, applying pyrosequencing of PCR-amplified V1-V4 16S rRNA gene regions. We analysed the resident bacterial communities in female and male scallop gonads before and after spawning. DNA samples were amplified and quality-filtered reads were assigned to family and genus taxonomic levels using the Ribosomal Database Project classifier. A total of 18,520 sequences were detected, belonging to 13 phyla, including Proteobacteria (55%), Bacteroidetes (11,7%), Firmicutes (3%), Actinobacteria (2%) and Spirochaetes (1,2%), and 110 genera...
June 2016: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/27845495/unicellular-but-not-asocial-life-in-community-of-a-bacterium
#5
Diego Romero
All living organisms have acquired the outstanding ability to overcome the limitations imposed by changeable environments through the gain of genetic traits over years of evolution and the tendency of individuals to associate in communities. The complementation of a singular weakness, the deployment of reinforcement for the good of the community, the better use of resources, or effective defense against external aggression are advantages gained by this communal behavior. Communication has been the cohesive element prompting the global responses that promote efficiency in two features of any community: specialization in differentiated labor and the spatio-temporal organization of the environment...
June 2016: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/27845494/monitoring-of-airborne-biological-particles-in-outdoor-atmosphere-part-2-metagenomics-applied-to-urban-environments
#6
EDITORIAL
Andrés Núñez, Guillermo Amo de Paz, Alberto Rastrojo, Ana M García, Antonio Alcamí, A Montserrat Gutiérrez-Bustillo, Diego A Moreno
The air we breathe contains microscopic biological particles such as viruses, bacteria, fungi and pollen, some of them with relevant clinic importance. These organisms and/or their propagules have been traditionally studied by different disciplines and diverse methodologies like culture and microscopy. These techniques require time, expertise and also have some important biases. As a consequence, our knowledge on the total diversity and the relationships between the different biological entities present in the air is far from being complete...
June 2016: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/27762430/antimicrobial-activity-of-lactobacillus-strains-of-chicken-origin-against-bacterial-pathogenss
#7
Marta Dec, Andrzej Puchalski, Anna Nowaczek, Andrzej Wernicki
This study was conducted to identify and evaluate the antimicrobial activity of some Lactobacillus isolates of chicken origin. Among 90 isolates 14 Lactobacillus species were distinguished using MALDI-TOF mass spectrometry and 16S-ARDRA. The dominant species was L. salivarius (34.4%), followed by L. johnsonii (23.3%), L. crispatus (13.3%) and L. reuteri (11.1%). All lactobacilli were screened for antimicrobial activity against wild-type strains of Salmonella enterica, Escherichia coli, and Clostridium perfringens...
March 2016: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/27762429/a-functional-ferric-uptake-regulator-fur-protein-in-the-fish-pathogen-piscirickettsia-salmonis
#8
Oscar Almarza, Katherine Valderrama, Manuel Ayala, Cristopher Segovia, Javier Santander
Piscirickettsia salmonis, a Gram-negative fastidious facultative intracellular pathogen, is the causative agent of the salmonid rickettsial septicemia (SRS). The P. salmonis iron acquisition mechanisms and its molecular regulation are unknown. Iron is an essential element for bacterial pathogenesis. Typically, genes that encode for the iron acquisition machinery are regulated by the ferric uptake regulator (Fur) protein. P. salmonis fur sequence database reveals a diversity of fur genes without functional verification...
March 2016: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/27762427/prevalence-of-campylobacter-spp-in-diarrhoea-samples-from-patients-in-new-south-wales-australia
#9
Aruna Devi, Adam S Gunjilac, Jenny M Wilkinson, Thiru Vanniasinkam, Timothy J Mahony
Campylobacteriosis is a leading cause of bacterial foodborne disease in many industrialized countries including Australia. New South Wales (NSW) is the most populous state in Australia yet the lack of any Campylobacter species surveillance programs has led to a knowledge gap in the importance of these pathogens as causes of diarrhoea. The data collected in this study demonstrated a need for such programs. In this study, 400 human clinical fecal samples were collected from two NSW locations, Western Sydney and Wagga Wagga, and tested for the presence of Campylobacter spp...
March 2016: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/27762425/haloalkalitolerant-actinobacteria-with-capacity-for-anthracene-degradation-isolated-from-soils-close-to-areas-with-oil-activity-in-the-state-of-veracruz-mexico
#10
Reyna Del C Lara-Severino, Miguel A Camacho-López, Edgar Casanova-González, Leobardo M Gómez-Oliván, Ángel H Sandoval-Trujillo, Keila Isaac-Olivé, Ninfa Ramírez-Durán
The use of native strains of microorganisms from soils is an excellent option for bioremediation. To our knowledge, until now there has been no other group working on the isolation of Actinobacteria from contaminated soils in Mexico. In this study, samples of soils close to areas with oil activity in the State of Veracruz, Mexico, were inoculated for the isolation of Actinobacteria. The strains isolated were characterized morphologically, and the concentrations of NaCl and pH were determined for optimal growth...
March 2016: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/27762424/monitoring-of-airborne-biological-particles-in-outdoor-atmosphere-part-1-importance-variability-and-ratios
#11
Andrés Núñez, Guillermo Amo de Paz, Alberto Rastrojo, Ana M García, Antonio Alcamí, A Montserrat Gutiérrez-Bustillo, Diego A Moreno
The first part of this review ("Monitoring of airborne biological particles in outdoor atmosphere. Part 1: Importance, variability and ratios") describes the current knowledge on the major biological particles present in the air regarding their global distribution, concentrations, ratios and influence of meteorological factors in an attempt to provide a framework for monitoring their biodiversity and variability in such a singular environment as the atmosphere. Viruses, bacteria, fungi, pollen and fragments thereof are the most abundant microscopic biological particles in the air outdoors...
March 2016: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/27762428/de-novo-synthesis-and-functional-analysis-of-the-phosphatase-encoding-gene-aci-b-of-uncultured-actinobacteria-from-lake-stechlin-ne-germany
#12
Abhishek Srivastava, Katherine D McMahon, Ramunas Stepanauskas, Hans-Peter Grossart
The National Center for Biotechnology Information [http://www.ncbi.nlm.nih.gov/guide/taxonomy/] database enlists more than 15,500 bacterial species. But this also includes a plethora of uncultured bacterial representations. Owing to their metabolism, they directly influence biogeochemical cycles, which underscores the the important status of bacteria on our planet. To study the function of a gene from an uncultured bacterium, we have undertaken a de novo gene synthesis approach. Actinobacteria of the acI-B subcluster are important but yet uncultured members of the bacterioplankton in temperate lakes of the northern hemisphere such as oligotrophic Lake Stechlin (NE Germany)...
December 2015: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/27762426/genetic-and-serologic-surveillance-of-rotavirus-with-p-8-and-p-4-genotypes-in-feces-from-children-in-the-city-of-chihuahua-northern-mexico
#13
Juan F Contreras-Cordero, César I Romo-Sáenz, Griselda E Menchaca-Rodríguez, Rocío Infante-Ramírez, Licet Villarreal-Treviño, Carlos E Hernández-Luna, Cristina Rodríguez-Padilla, Reyes S Tamez-Guerra
Rotavirus vaccine was developed using the most prominent G and P genotypes circulating in children population. Therefore, severe gastroenteritis has been reduced around the world. This study investigated the G and P rotavirus genotypes circulating in children from two hospitals in the city of Chihuahua, Mexico. Additionally, polyclonal antibodies against Rotavirus Wa strain were used to determine their homotypic and heterotypic reactivity to both P[8] and P[4] genotypes. G1, G2, and G3 VP7 genotypes and P[8] and P[4] VP4 genotypes were detected in common and uncommon combinations as well as mixed infectious...
December 2015: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/27611678/hypercompetition-in-biomedical-research-evaluation-and-its-impact-on-young-scientist-careers
#14
Shina Caroline Lynn Kamerlin
Recent years have seen tremendous changes in the modes of publication and dissemination of biomedical information, with the introduction of countless new publishers and publishing models, as well as alternative modes of research evaluation. In parallel, we are witnessing an unsustainable explosion in the amount of information generated by each individual scientist, at the same time as many countries' shrinking research budgets are greatly increasing the competition for research funding. In such a hypercompetitive environment, how does one measure excellence? This contribution will provide an overview of some of the ongoing changes in authorship practices in the biomedical sciences, and also the consequences of hypercompetition to the careers of young scientists, from the perspective of a tenured young faculty member in the biomedical sciences...
December 2015: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/27611677/interaction-and-cooperative-effort-among-scientific-societies-twelve-years-of-cosce
#15
Nazario Martín, Carlos Andradas
The evolution of knowledge and technology in recent decades has brought profound changes in science policy, not only in the countries but also in the supranational organizations. It has been necessary, therefore, to adapt the scientific institutions to new models in order to achieve a greater and better communication between them and the political counterparts responsible for defining the general framework of relations between science and society. The Federationon of Scientific Societies of Spain (COSCE, Confederación de Sociedades Científicas de España) was founded in October 2003 to respond to the urgent need to interact with the political institutions and foster a better orientation in the process of making decisions about the science policy...
December 2015: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/27611676/a-kluyveromyces-marxianus-2-deoxyglucose-resistant-mutant-with-enhanced-activity-of-xylose-utilization
#16
Suprayogi Suprayogi, Minh T Nguyen, Noppon Lertwattanasakul, Nadchanok Rodrussamee, Savitree Limtong, Tomoyuki Kosaka, Mamoru Yamada
Thermotolerant ethanologenic yeast Kluyveromyces marxianus is capable of fermenting various sugars including xylose but glucose represses to hamper the utilization of other sugars. To acquire glucose repression-defective strains, 33 isolates as 2-deoxyglucose (2-DOG)-resistant mutants were acquired from about 100 colonies grown on plates containing 2-DOG, which were derived from an efficient strain DMKU 3-1042. According to the characteristics of sugar consumption abilities and cell growth and ethanol accumulation along with cultivation time, they were classified into three groups...
December 2015: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/27611675/chlamydia-pneumoniae-cpj0783-interaction-with-huntingtin-protein14
#17
Izumi Yanatori, Yumiko Yasui, Kazunobu Ouchi, Fumio Kishi
Chlamydia pneumoniae is a Gram-negative, obligate intracellular pathogen that causes community-acquired respiratory infections. After C. pneumoniae invades host cells, it disturbs the vesicle transport system to escape host lysosomal or autophagosomal degradation. By using a yeast mis-sorting assay, we found 10 C. pneumoniae candidate genes involved in aberrant vesicular trafficking in host cells. One of the candidate genes, CPj0783, was recognized by antibodies from C. pneumoniae-infected patients. The expression of CPj0783 was detected at mid to late-cycle time points and increased during the inclusion maturation...
December 2015: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/27611674/archaeal-and-bacterial-diversity-in-two-hot-springs-from-geothermal-regions-in-bulgaria-as-demostrated-by-16s-rrna-and-gh-57-genes
#18
Katerina Stefanova, Iva Tomova, Anna Tomova, Nadja Radchenkova, Ivan Atanassov, Margarita Kambourova
Archaeal and bacterial diversity in two Bulgarian hot springs, geographically separated with different tectonic origin and different temperature of water was investigated exploring two genes, 16S rRNA and GH-57. Archaeal diversity was significantly higher in the hotter spring Levunovo (LV) (82°C); on the contrary, bacterial diversity was higher in the spring Vetren Dol (VD) (68°C). The analyzed clones from LV library were referred to twenty eight different sequence types belonging to five archaeal groups from Crenarchaeota and Euryarchaeota...
December 2015: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/27611673/chromosomes-of-protists-the-crucible-of-evolution
#19
Marie-Odile Soyer-Gobillard, Michael F Dolan
As early as 1925, the great protozoologist Edouard Chatton classified microorganisms into two categories, the prokaryotic and the eukaryotic microbes, based on light microscopical observation of their nuclear organization. Now, by means of transmission electron microscopy, we know that prokaryotic microbes are characterized by the absence of nuclear envelope surrounding the bacterial chromosome, which is more or less condensed and whose chromatin is deprived of histone proteins but presents specific basic proteins...
December 2015: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/27611672/year-s-comments-for-2015
#20
EDITORIAL
Ricardo Guerrero
No abstract text is available yet for this article.
December 2015: International Microbiology: the Official Journal of the Spanish Society for Microbiology
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