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International Microbiology: the Official Journal of the Spanish Society for Microbiology

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https://www.readbyqxmd.com/read/28617527/impact-of-motility-and-chemotaxis-features-of-the-rhizobacterium-pseudomonas-chlororaphis-pcl1606-on-its-biocontrol-of-avocado-white-root-rot
#1
Álvaro Polonio, Carmen Vida, Antonio de Vicente, Francisco M Cazorla
The biocontrol rhizobacterium Pseudomonas chlororaphis PCL1606 has the ability to protect avocado plants against white root rot produced by the phytopathogenic fungus Rosellinia necatrix. Moreover, PCL1606 displayed direct interactions with avocado roots and the pathogenic fungus. Thus, nonmotile (flgK mutant) and non-chemotactic (cheA mutant) derivatives of PCL1606 were constructed to emphasize the importance of motility and chemotaxis in the biological behaviour of PCL1606 during the biocontrol interaction...
June 2017: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/28617526/antimicrobial-resistance-gene-expression-associated-with-multidrug-resistant-salmonella-spp-isolated-from-retail-meat-in-hanoi-vietnam
#2
Minh Ngoc Nghiem, Viet Thanh Nguyen, Thu Thi Hoai Nguyen, Ton Dang Nguyen, Thuy Thi Bich Vo
The purpose of this study was to further characterize the multi-antimicrobial resistance and antibiotic resistance gene expression associated with multi-drug resistance (MDR) in Salmonella spp. isolates from retail meats in Hanoi, Vietnam. A total of 14 Salmonella spp. belonging to 9 serotypes (e.g., Warragul, London, Derby, Indiana, Meleagridis, Give, Rissen, Assine, and Typhimurium) were tested for sensitivity to 8 antibiotics. Resistance to at least one antibiotic was shown in 13 strains (92.85%). The multiple antimicrobial resistances accounted for 64...
June 2017: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/28617525/characterization-of-high-exopolysaccharide-producing-lactobacillus-strains-isolated-from-mustard-pickles-for-potential-probiotic-applications
#3
Jing-Yao Huang, Cheng-Yen Kao, We-Sin Liu, Tony J Fang
The aim of this study was to characterize high exopolysaccharide (EPS)-producing lactic acid bacteria (LAB) isolated from mustard pickles in Taiwan for potential probiotic applications. Among 39 collected LAB strains, four most productive EPS-producing strains were selected for further analysis. Comparative analyses of 16S rDNA genes rpoA and pheS sequences demonstrated that these strains were members of Lactobacillus plantarum-group (LPG). NCD 2, NLD 4, SLC 13, and NLD 16 showed survival rates of 95.83% ± 0...
June 2017: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/28617524/high-prevalence-of-methicillin-resistant-coagulase-negative-staphylococci-isolated-from-a-university-environment-in-thailand
#4
Rathanin Seng, Udomluk Leungtongkam, Rapee Thummeepak, Wassana Chatdumrong, Sutthirat Sitthisak
The present study was conducted to isolate and characterize the molecular epidemiology of the methicillin-resistant staphylococci in the general university environment, where all five locations; the library, restrooms, canteens, computer rooms and outdoor surfaces, are in common use by a large population of students. We used Mannitol Salt Agar (MSA) supplemented with 4 μg/ml of oxacillin to screen the methicillin-resistant staphylococci. The species level was identified by PCR of rdr (Staphylococcus epidermidis), groESL (Staphylococcus haemolyticus) and nuc (Staphylococcus aureus and Staphylococcus warneri) genes and DNA sequencing of tuf and dnaJ genes...
June 2017: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/28617523/electrochemical-characterization-of-geobacter-lovleyi-identifies-limitations-of-microbial-fuel-cell-performance-in-constructed-wetlands
#5
Clara Corbella, Rebecca P Steidl, Jaume Puigagut, Gemma Reguera
Power generation in microbial fuel cells implemented in constructed wetlands (CW-MFCs) is low despite the enrichment of anode electricigens most closely related to Geobacter lovleyi. Using the model representative G. lovleyi strain SZ, we show that acetate, but not formate or lactate, can be oxidized efficiently but growth is limited by the high sensitivity of the bacterium to oxygen. Acetate and highly reducing conditions also supported the growth of anode biofilms but only at optimal anode potentials (450 mV vs...
June 2017: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/28581021/antimicrobial-peptide-from-bacillus-subtilis-csb138-characterization-killing-kinetics-and-synergistic-potency
#6
Sudip Regmi, Yoon Seok Choi, Yun Hee Choi, Young Kyun Kim, Seung Sik Cho, Jin Cheol Yoo, Joo-Won Suh
We studied the prospect of synergy between the antimicrobial peptide p138c and non-peptide antibiotics for increasing the potency and bacterial killing kinetics of these agents. The production of p138c was maximized in the late exponential growth phase of Bacillus subtilis CSB138. Purification of p138c resulted in a total of 4800 arbitrary units (AU) with 19.15-fold and 3.2% recovery. Peptide p138c was thermo-tolerant up to 50 °C and stable at pH 5.8 to 11. The biochemical nature of p138c was determined by a bioassay, similar to tricine-SDS-PAGE, indicating inhibition at 3 kDa...
March 2017: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/28581020/insights-into-the-fecal-microbiota-of-captive-iberian-lynx-lynx-pardinus
#7
José Alberto Núñez-Díaz, M Carmen Balebona, Eva M Alcaide, Irene Zorrilla, Miguel Ángel Moriñigo
The Iberian lynx (Lynx pardinus) is an endangered species restricted to several areas of Spain and Portugal. Its low genetic diversity likely provokes immune depression and high susceptibility to infectious diseases. The intestinal microbiota is closely related to host health and nutrition. In order to contribute to the knowledge of the Iberian lynx intestinal microbiota, fecal microbiota of captive specimens from two breeding centers ("La Olivilla" and "El Acebuche"), located in Southern Spain, were studied by Denaturing Gradient Gel Electrophoresis (DGGE)...
March 2017: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/28581019/identification-by-using-maldi-tof-mass-spectrometry-of-lactic-acid-bacteria-isolated-from-non-commercial-yogurts-in-southern-anatolia-turkey
#8
Ayse Karaduman, Mehmet Ozaslan Ozaslan, Ibrahim H Kilic, Sibel Bayil-Oguzkan, Bekir S Kurt, Nese Erdogan
Yogurt is a dairy product obtained by bacterial fermentation of milk. Commercial yogurts are produced using standard starters while, in the production of non-commercial yogurt, the microbiota is quite different since yogurts are used as starter for years. To determine the final characteristics of the fermented product it is necessary to know the biochemical properties of the starter cultures, such as acidity, aroma and flavor. This can only be achieved by identifying and characterizing the bacteria in starter cultures...
March 2017: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/28581018/impact-of-pollution-on-the-microbial-diversity-of-a-tropical-river-in-an-urbanized-region-of-northeastern-brazil
#9
Thorsten Köchling, José Luis Sanz, Luiz Galdino, Lourdinha Florencio, Mario T Kato
Rivers are important ecosystems that are integrated into biogeochemical cycles and constitute an essential resource for numerous human uses. However, the assessment of the biological diversity and composition of microbial communities found in rivers remains incomplete, partly due to methodological constraints which are only recently being resolved with the advent of next generation sequencing (NGS) techniques. Using 454-pyrosequencing of the 16S gene, the present study analyzed the microbial diversity of the planktonic and sediment populations in a tropical river in northeastern Brazil that is exposed to severe pollution...
March 2017: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/28581017/revisiting-the-genus-photobacterium-taxonomy-ecology-and-pathogenesis
#10
Alejandro M Labella, David R Arahal, Dolores Castro, Manuel L Lemos, Juan J Borrego
The genus Photobacterium, one of the eight genera included in the family Vibrionaceae, contains 27 species with valid names and it has received attention because of the bioluminescence and pathogenesis mechanisms that some of its species exhibit. However, the taxonomy and phylogeny of this genus are not completely elucidated; for example, P. logei and P. fischeri are now considered members of the genus Aliivibrio, and previously were included in the genus Vibrio. In addition, P. damselae subsp. piscicida was formed as a new combination for former Vibrio damsela and Pasteurella piscicida...
March 2017: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/28504818/characterization-of-escherichia-coli-clinical-isolates-causing-urinary-tract-infections-in-the-community-of-chilpancingo-mexico
#11
Jesús A Hernández-Vergara, Verónica I Martínez-Santos, Romina B Radilla-Vázquez, Jesús Silva-Sánchez, Amalia Vences-Velásquez, Natividad Castro-Alarcón
Escherichia coli is the main cause of urinary tract infections (UTI) in ambulatory patients, especially strains belonging to the B2 phylogenetic group and ST131 clonal group. Antibiotic treatment is usually administered empirically; however, it is not always effective due to bacterial multidrug resistance and the production of extended spectrum β-lactamases (ESBLs). The aim of this study was to characterize E. coli clinical isolates from patients with UTI in a community of the State of Guerrero, Mexico. From January to August 2014, 134 clinical isolates of E...
December 2016: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/28504817/biofilm-formation-in-catheter-related-infections-by-panton-valentine-leukocidin-producing-staphylococcus-aureus
#12
Giorgio Silva-Santana, Kátia C Lenzi-Almeida, Vânia G S Lopes, Fábio Aguiar-Alves
The use of invasive techniques, such as intravascular catheter insertion, and the formation of biofilms in several devices by methicillin-resistant Staphylococcus aureus (MRSA) have contributed to the increased number of septic patients, morbidity and mortality. This study aimed to evaluate the virulence of strains through catheter colonization and identification of microbial biofilm, as well as pathological changes on the colonized skin. An experimental biofilm formation model utilized catheter fragments implanted subcutaneously in 25 Swiss mice...
December 2016: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/28504816/virulence-factors-and-antimicrobial-resistance-in-environmental-strains-of-vibrio-alginolyticus
#13
Marcos F Hernández-Robles, Ana K Álvarez-Contreras, Patricia Juárez-García, Iván Natividad-Bonifacio, Everardo Curiel-Quesada, Carlos Vázquez-Salinas, Elsa I Quiñones-Ramírez
Vibrio alginolyticus has acquired increasing importance because this microorganism may be pathogenic to aquatic animals and humans. It has been reported that some V. alginolyticus strains carry virulence genes derived from pathogenic V. cholerae and V. parahaemolyticus strains. In this work V. alginolyticus was isolated from oyster samples acquired from a food-market in Mexico City. Thirty isolates were identified as V. alginolitycus. Strains showed β-haemolysis and proteolytic activity and produced a capsule...
December 2016: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/28504815/the-arago-laboratory-of-banyuls-and-some-of-its-academicians
#14
Marie-Odile Soyer-Gobillard
Since its founding in 1881 by Henri de Lacaze-Duthiers (1821-1901), the Arago Laboratory of Banyuls has been one of the three marine stations of the University Pierre and Marie Curie-Paris 6. It is located in Banyuls (Banyuls-sur-Mer) in Northern Catalonia. The center hosts researchers and students from all over the world. Some became famous, including four Nobel Prize winners: André Lwoff (1965), Pierre-Gilles de Gennes (1991), Albert Fert (2007) and Jules Hoffmann (2011). This article focuses on five scientists closely related to the center...
December 2016: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/28494087/investigation-of-twenty-selected-medicinal-plants-from-malaysia-for-anti-chikungunya-virus-activity
#15
Yik Sin Chan, Kong Soo Khoo, Nam Weng Weng Sit
Chikungunya virus is a reemerging arbovirus transmitted mainly by Aedes mosquitoes. As there are no specific treatments available, Chikungunya virus infection is a significant public health problem. This study investigated 120 extracts from selected medicinal plants for anti-Chikungunya virus activity. The plant materials were subjected to sequential solvent extraction to obtain six different extracts for each plant. The cytotoxicity and antiviral activity of each extract were examined using African monkey kidney epithelial (Vero) cells...
September 2016: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/28494086/functional-ecology-of-soil-microbial-communities-along-a-glacier-forefield-in-tierra-del-fuego-chile
#16
Miguel A Fernández-Martínez, Stephen B Pointing, Sergio Pérez-Ortega, María Arróniz-Crespo, T G Allan Green, Ricardo Rozzi, Leopoldo G Sancho, Asunción de Los Ríos
A previously established chronosequence from Pia Glacier forefield in Tierra del Fuego (Chile) containing soils of different ages (from bare soils to forest ones) is analyzed. We used this chronosequence as framework to postulate that microbial successional development would be accompanied by changes in functionality. To test this, the GeoChip functional microarray was used to identify diversity of genes involved in microbial carbon and nitrogen metabolism, as well as other genes related to microbial stress response and biotic interactions...
September 2016: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/28494085/atypical-enteropathogenic-escherichia-coli-aepec-in-children-under-five-years-old-with-diarrhea-in-quito-ecuador
#17
Francisco X Mora, Rolando X Avilés-Reyes, Laura Guerrero-Latorre, Esteban Fernández-Moreira
Enteropathogenic Escherichia coli (EPEC) remain one the most important pathogens infecting children and they are one of the main causes of persistent diarrhea worldwide. In this study, we have isolated EPEC from 94 stool samples of children under five years old with diarrheal illness in the area of Quito (Ecuador), and we have determined the occurrence of the two subtypes of EPEC, typical EPEC (tEPEC) and atypical (aEPEC), by PCR amplification of the genes eae (attaching and effacing) and bfp (bundle- forming pilus)...
September 2016: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/28494084/the-use-of-high-throughput-dna-sequence-analysis-to-assess-the-endophytic-microbiome-of-date-palm-roots-grown-under-different-levels-of-salt-stress
#18
Mahmoud W Yaish, Ibtisam Al-Harrasi, Aliya S Alansari, Rashid Al-Yahyai, Bernard R Glick
Date palms are able to grow under diverse abiotic stress conditions including in saline soils, where microbial communities may be help in the plant's salinity tolerance. These communities able to produce specific growth promoting substances can enhance date palm growth in a saline environment. However, these communities are poorly defined. In the work reported here, the date palm endophytic bacterial and fungal communities were identified using the pyrosequencing method, and the microbial differential abundance in the root upon exposure to salinity stress was estimated...
September 2016: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/28494083/phosphate-from-stardust-to-eukaryotic-cell-cycle-control
#19
Javier Jiménez, Samuel Bru, Mariana Pc Ribeiro, Josep Clotet
Phosphorus is a pivotal element in all biochemical systems: it serves to store metabolic energy as ATP, it forms the backbone of genetic material such as RNA and DNA, and it separates cells from the environment as phospholipids. In addition to this "big hits", phosphorus has recently been shown to play an important role in other important processes such as cell cycle regulation. In the present review, we briefly summarize the biological processes in which phosphorus is involved in the yeast Saccharomyces cerevisiae before discussing our latest findings on the role of this element in the regulation of DNA replication in this eukaryotic model organism...
September 2016: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/27845499/distribution-of-virulence-genes-involved-in-biofilm-formation-in-multi-drug-resistant-acinetobacter-baumannii-clinical-isolates
#20
Rapee Thummeepak, Phattaraporn Kongthai, Udomluk Leungtongkam, Sutthirat Sitthisak
Acinetobacter baumannii is an opportunistic bacterial pathogen that is the major cause of hospital-acquired infections. It has been shown that A. baumannii with high biofilm formation increases the risk of acquiring infection. In this study, the prevalence of virulence genes involved in biofilm formation was determined in 225 A. baumannii clinical isolates from three hospitals in Thailand. Most of the isolates were multidrug-resistant A. baumannii strains (86.2%). Among all isolates, 76.9% (173/225) showed biofilm formation ability...
June 2016: International Microbiology: the Official Journal of the Spanish Society for Microbiology
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