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Egyptian Journal of Immunology

Mohamed S Badari, Sherein G Elgendy, Asmaa S Mohamed, Alaa T Hassan
The immunomodulatory effects of antibiotics could influence the degree of systemic and local responses to infection, so investigation of their intrinsic influence on the host's inflammatory response appears to be essential. Fluoroquinolones are known to exert modulatory activity on immune responses to microbial infection. However the mechanism of this immunmodulation has not been well elucidated. The aim of the work, is to assess the immunomodulatory effects of a levofloxacin, through examining its effect on the concentrations of tumor necrosis factor α (TNF-α) and Interleukin - 10 (IL-10) in serum of pneumonic patients...
2015: Egyptian Journal of Immunology
Amel A El Naggar, Mostafa El-Naggar, El-Hassan Mokhamer, Mona W Avad
The measurement of serum free light chain (sFLC) has been shown to be valuable in screening for the presence of plasma cell dyscrasia as well as for baseline prognosis in newly diagnosed patients. The aim of the present work was to study the prognostic value of sFLC in multiple myeloma in relation to other serum biomarkers, response to therapy and survival. Forty five newly diagnosed patients with MM were included in the study. Patients were divided into responders and non-responders groups according to response to therapy...
2015: Egyptian Journal of Immunology
M A El Bassuoni, M A Soliman, N A El Megeed, A Al Gazar
Retinoic acid-related orphan receptor gT (RORγT) is the orphan nuclear receptor that regulates the development of Th17 cells and the expression of IL-17. The differentiation of Th17 cells is associated with the upregulation of RORγT mRNA, and the mechanisms regulating that process in human cells are not well understood. RORγt as transcription factor was selectively expressed in Th17 cells and is regulated by STAT3. The relationship between Th17 cells and tumor immunopathology has been controversial. Aim of the study is to evaluate Th17 cells and RORγt transcriptional factor in cirrhotic, early and advanced HCC patients...
2015: Egyptian Journal of Immunology
Roshdan M Arafa, Somaya M Desouky, Sherin M Emam, Neveen Tawfik Abed, Sahar Y Mohamed
Type 1 diabetes is one of the most common chronic childhood illnesses. Interplay between genetic susceptibility and environmental factors is thought to provide the fundamental element for the disease. It has been shown that more than 40 genetic loci are associated with T1DM. Important one among these is the CTLA-4. This work aimed to detect Cytotoxic T Lymphocyte-associated antigen 4 (CTLA-4) gene polymorphism in patients with type 1 diabetes mellitus T1DM using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to clarify its role in the susceptibility to T1DM...
2015: Egyptian Journal of Immunology
Nehad A Fouad, Sheref M Eltaher, Omminea A Abdullah, Ramy A Metwally
Growth arrest-specific protein 6 (Gas6) belongs to a family of vitamin K-dependent coagulation proteins. Plasma levels of Gas6 are associated to altered glucose tolerance. This study aimed at determining whether c.843+7G>A Gas6 polymorphism is associated with the development of type 2 diabetes mellitus. The study included 50 patients with type 2 diabetes and 40 matched controls. The Gas6 protein was measured in serum using ELISA and Gas6 gene polymorphism by polymerase chain reaction-Restriction Fragment Length Polymorphism...
2015: Egyptian Journal of Immunology
Reem R Abd El-Glil, Effat H Assar
Immune thrombocytopenic purpura (ITP) is an immune-mediated acquired disease with transient or persistent decrease of thrombocytes number in the blood. Cytokines play important roles in the immune regulation and are known to be deregulated in autoimmune diseases. This study aimed to investigate serum IL-16 levels in relation to reticulated platelets in children with ITP and platelet count. Twenty six children with ITP (11 with newly diagnosed ITP, 9 with persistent ITP and 6 with chronic ITP) and 12 age-matched healthy children controls were studied...
2015: Egyptian Journal of Immunology
Manal M S Youssef, Emma C Paish, J Clifford Murray, Naglaa M Farag, Khaled Saleh, I O Ellis
Dysfunction of the immune system in colorectal cancer (CRC) can be due to a number of reasons including apoptosis of tumour infiltrating lymphocytes (TILs). The aims of this study was to investigate TILs in colorectal cancer and characterize apoptosis of TILs using terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay for detecting DNA fragments. We used monoclonal antibodies (mAbs) to T lymphocytes to detect TILs and double immunohistochemistry to assess apoptosis. T lymphocytes were detected in the immune infiltrate in CRC...
2015: Egyptian Journal of Immunology
Ahlam M Ismail, Sanaa S Aly, Hanan M Fayed, Samar S Ahmed
Bronchial asthma (BA) is one of the common chronic diseases of childhood. Vitamin D deficiency has been associated with BA. Suppressor regulatory T cells (Treg) are important for the induction, maintenance of immunological tolerance to allergens. This study assessed serum 25-hydroxyvitamin D (vitamin D) and the percentages of CD4+CD25+(high) Foxp3+ Treg, in peripheral blood, as predictors of asthma severity and level of clinical control. The study enrolled 72 children divided equally between asthmatic children (AC) and age and sex matched controls...
2015: Egyptian Journal of Immunology
Salma Y Abu El-Nazar, Amany A Ghazy, Hossam E Ghoneim, Malak Zoheir, Ahmed S Ahmed, Sally S Sorour, Amira M Abouelella
Breast cancer is the most common gynecological malignancy in the world. In Egypt, it ranks the first among female malignancies with incidence of 37.7%. Over the last decades, the integration of prognostic and predictive markers in treatment decisions has led to more individualized and optimized therapy. NY-BR-1 antigen has been shown to be frequently expressed in breast cancers. The study aimed to assess the tissue expression of NY-BR-1 antigen and serum IgG antibody to this antigen in Egyptian breast cancer females...
2015: Egyptian Journal of Immunology
Asmaa S M Abdel-Rehim, Dina S Sheha, Nesrine A Mohamed
Vitamin D has an important role in the immune system. Decreased serum vitamin D level is known to be associated with autoimmune and atopic diseases. This study aimed to assess vitamin D status in patients with chronic spontaneous urticaria (CSU) and its relation with severity of the disease. This case-control study was conducted on 22 patients and 20 age and sex matched controls. Patients were subjected to clinical assessment, routine laboratory examination: complete blood picture (CBC), erythrocyte sedimentation rate (ESR), hepatitis B surface antigen (HBsAg), hepatitis C virus antibody (HCV Ab), anti-thyroid antibody, anti-nuclear antibody (ANA), total immunoglobulin E (IgE), vitamin D assay and stool analysis, and special investigations including: skin prick test (SPT), autologous serum skin test (ASST) and urticaria activity score (UAS)...
2014: Egyptian Journal of Immunology
Ahmed M Salman, Ida Rosenkrands, Peter Andersen, Abdel-Rahman B Abdel-Ghaffar
Tuberculosis (TB) remains one of the major challenges to the global public health. The most powerful tools in any TB control program are prompt diagnosis and successful treatment of patients with active contagious disease. For almost 100 years the tuberculin skin test (TST) has been used to support the diagnosis of active and latent TB infection. The TST has a number of limitations, most notable low specificity in BCG vaccinated individuals due to cross-reactive components in PPD and the Mycobacterium bovis BCG vaccine strain and an intensive search for new and more specific diagnostic antigens has therefore been ongoing...
2014: Egyptian Journal of Immunology
Manal M S Youssef, Emma C Paish, J Clifford Murray, Naglaa M Farag, Khaled Saleh, I O Ellis
Dysfunction of the immune system in colorectal cancer (CRC) can be due to a number of reasons including apoptosis of tumour infiltrating lymphocytes (TILs). The aims of this study were to characterize, phenotypically, the apoptosis of TILs in CRC, and define the association of these findings with prognostic indicators. We used double immunohistochemistry to assess the apoptosis of T-cell subsets. Monoclonal antibodies to T lymphocytes, T helper cells, cytotoxic T cells (CTLs), natural killer cells (NK), CD45 and CD45RO were used...
2014: Egyptian Journal of Immunology
Samah A Elshweikh, Radwa M Elkhouly, Mohamed M Shaalan, Safinaz H Shorbagy
Renal involvement is common in systemic lupus erythematosus (SLE). Anti-C1q antibodies are associated with SLE nephritis. This study attempted to correlate anti-C1q levels to different types of lupus nephritis (LN). Anti-C1q antibodies were assessed in two groups (15 subjects each) of SLE patients with and without LN. Ten apparently healthy volunteers served as controls. The sensitivity of anti C1q for diagnosis of lupus nephritis among SLE patients was 100.00% and the specificity reached 90.00%. The positive and negative predictive values for renal activity were 83...
2014: Egyptian Journal of Immunology
S Abo-Elnazar, M Moaaz, H Ghoneim, T Molokhia, W El-Korany
Opioids are widely used for the treatment of severe pain. However, opioids, particularly morphine, is known to cause immunosuppression. This study investigated the impact of morphine and cannabinoids addiction on CD4+ T cell mediated immunity. We hypothesize that, accompanied immunosuppression is attributed to reduced T cell activation with an extent of affection to the cytoplasmic activity of the biologically active transcription factor nuclear factor-κB (NF-κB) which play crucial role in T-cell activation...
2014: Egyptian Journal of Immunology
Sawsan Said Hafez, Wessam El Sayed Saad, Noha Hussien Shedid
Systemic lupus erythematosus (SLE) is a prototype of systemic autoimmune disease in which cytokines such as B lymphocyte chemoattractant (BLC or CXCL13) may play important roles in pathogenesis. The aim of our study was to investigate the implications of CXCL13 in SLE diagnosis and its correlation with disease activity and renal involvement. The study included 50 adult female patients with SLE and 30 age-matched female healthy individuals serving as a control group. Patients' Group was further subdivided according to disease activity calculated by SLE Disease Activity Index (SLEDAI)...
2014: Egyptian Journal of Immunology
Sohair K Sayed, Sherif H Galal, Omar M Herdan, Ayman M Mahran
Transforming growth factor β1 (TGF-β1) has a large role in the control of autoimmunity. Single nucleotide polymorphisms (SNP) in the promoter of TGF-β1 cytokine gene are known to alter the production of this important cytokine. Decreased levels of TGF-β1 may contribute to systemic lupus erythematosus (SLE) susceptibility, activity and organ damage. Lupus nephritis (LN) occurs in more than one-third of patients with SLE. In this study we measured serum levels of TGF-β1 and assessed TGF-β1 single nucloetide polymorphism (SNP) at codon 10 (T869C) in Egyptian SLE population in order to verify whether there is a relationship between this polymorphism, serum level of TGF-β1, SLE susceptibility, clinical manifestations and lupus nephritis...
2014: Egyptian Journal of Immunology
Douaa Sayed, Omnia H B El-Badawy, Eman Nasr Eldin, Rania Bakry, Mohamed S Badary, Mohamed E Abd-Alrahman, Mohamed A El-Feky, Amany G Thabit
To track the changes in the tested Treg markers especially Foxp3 following activation to determine whether data of human studies using Foxp3 in evaluation of Tregs are reliable or not. Four-colour flow cytometry analysis was carried out to calculate the percentages of Tregs before and after lymphocyte activation. Foxp3 expression by CD4(+)CD25(+)* and CD4(+)CD25(high) T cells increased after T cell activation. A moderate negative correlation was observed between the percentage of each of CD4(+)CD25(+)Foxp3(+)IL10(+) or CD4(+)CD25(high) Foxp3(+)IL10(+) T cells and the percentage of CD4(+)CD25(+) T cells "after activation" and a weak negative correlation was similarly observed between the percentage of CD4(+)CD25(-)Foxp3(+)IL10(+) T cells and the percentage of CD4(+)CD25(+) T cells "after activation"...
2014: Egyptian Journal of Immunology
Ghada Boghdadi, Waseem Mohamed Seleem
Toll-like receptors (TLRs) have been identified as key regulators of innate and adaptive immune responses in viral infection. The contribution of the host immune response to sustained virologic response is not clear in patients with chronic hepatitis C virus (HCV) infection. This study aimed to investigate the expression of TLRs 7 & 8 mRNA in monocytes of patients with chronic (HCV) infection showing different responses to interferon and ribavirin treatment. The study group was comprised of 21 chronic HCV infected patients...
2014: Egyptian Journal of Immunology
Eman R Abdel Gawad, Amira I Mansour, Yasser A Abdel Aziz, Aml F Soliman, Rasha M Fawzy
SLE is a heterogeneous disease, characterized by variation in clinical and serological manifestations. This study intended to verify the role of anti-nucleosome (anti-NCS) antibodies in diagnosis of SLE and lupus nephritis and to establish the correlation between antibody reactivity and disease activity. Study subjects included 35 patients diagnosed as SLE and two control groups. While the first is a group with other collagen diseases (n = 80), the second included 50 matched apparently healthy subjects. Serum IgG anti-NCS antibodies in all subjects were detected by ELISA...
2014: Egyptian Journal of Immunology
Neveen A Abedel Hafeez, Mona El-Toukhy Fouda, Eman R Abdel Gawad, Tamer Assar, Amira I Mansour
Preeclampsia is a multisystem disorder of unknown cause that is unique to human pregnancy affecting about 7-10% of pregnant women. CD4+CD25(bright) regulatory T (Treg) cells have been identified as a principle regulator of tolerance during pregnancy. The aim of this study was to demonstrate the change of peripheral CD4+CD25(bright) regulatory T lymphocytes in normal pregnancy and preeclampsia, and to explore its role in the pathogenesis of preeclampsia. We determined CD4+CD25(bright) Treg cells in the peripheral blood using flow cytometry and forkhead box P3 (FoxP3+) cells at the peripheral blood using real time PCR...
2014: Egyptian Journal of Immunology
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