journal
https://read.qxmd.com/read/38327232/rapid-detection-of-mucorales-in-human-blood-and-urine-samples-by-functionalised-heusler-magnetic-nanoparticle-assisted-customized-loop-mediated-isothermal-amplification
#21
JOURNAL ARTICLE
Rahul Roy, Gagandeep Singh, Ujjwal Ranjan Dahiya, Mragnayani Pandey, Immaculata Xess, Dinesh Kalyanasundaram
BACKGROUND: Mucormycosis is a rare disease with scarce diagnostic methods for early intervention. Available strategies employing direct microscopy using calcofluor white-KOH, culture, radiologic, and histopathologic testing often are time-intensive and demand intricate protocols. Nucleic Acid Amplification Test (NAAT) holds promise due to its high sensitivity combined with rapid detection. Loop-mediated isothermal amplification (LAMP) based detection offers an ultrasensitive technique that does not require complicated thermocyclers like in PCR, offering a straightforward means for improving diagnoses as a near-point-of-care test...
February 7, 2024: Medical Mycology
https://read.qxmd.com/read/38318638/neutrophil-extracellular-traps-nets-and-th-2-dominant-immune-responses-in-chronic-granulomatous-chromobalstomycosis
#22
JOURNAL ARTICLE
Huan Huang, Minying Li, Mingfen Luo, Jinjin Zheng, Qian Li, Xiaoyue Wang, Yinghui Liu, Dongmei Li, Liyan Xi, Hongfang Liu
Chromoblastomycosis (CBM), a chronic, granulomatous, suppurative mycosis of the skin and subcutaneous tissue, is caused by several dematiaceous fungi. The formation of granulomas, tissue proliferation, and fibrosis in response to these pathogenic fungi is believed to be intricately linked to host immunity. To understand this complex interaction, we conducted a comprehensive analysis of immune cell infiltrates, neutrophil extracellular traps (NETs) formation, and the fibrosis mechanism in 20 CBM lesion biopsies, using immunohistochemical and immunofluorescence staining methods...
February 5, 2024: Medical Mycology
https://read.qxmd.com/read/38318635/a-novel-model-for-predicting-deep-seated-candidiasis-due-to-candida-glabrata-among-cancer-patients-a-six-year-study-in-a-cancer-center-of-china
#23
JOURNAL ARTICLE
Ding Li, Lin Wang, Zhihong Zhao, Changsen Bai, Xichuan Li
Followed by C. albicans, C. glabrata ranks as the second major species contributing to invasive candidiasis. Given the higher medical burden and lower susceptibility to azoles in C. glabrata infections, identifying these infections is critical. From 2016 to 2021, patients with deep-seated candidiasis due to C. glabrata and non-glabrata Candida met the criteria to be enrolled in the study. Clinical data were randomly divided into training and validation cohorts. A predictive model and nomogram were constructed using R software based on the stepwise algorithm and logistic regression...
February 5, 2024: Medical Mycology
https://read.qxmd.com/read/38308518/molecular-epidemiology-and-antimicrobial-resistance-of-vaginal-candida-glabrata-isolates-in-namibia
#24
JOURNAL ARTICLE
Cara M Dunaiski, Marleen M Kock, Wai Yin Chan, Arshad Ismail, Remco P H Peters
Candida glabrata is the most common non-albicans Candida species that causes vulvovaginal candidiasis (VVC). Given the intrinsically low susceptibility of C. glabrata to azole drugs, investigations into C. glabrata prevalence, fungal susceptibility profile, and molecular epidemiology are necessary to optimise the treatment of VVC. This molecular epidemiological study was conducted to determine antifungal drug profile, single nucleotide polymorphisms (SNPs) associated with phenotypic antifungal resistance and epidemic diversity of C...
February 2, 2024: Medical Mycology
https://read.qxmd.com/read/38289726/molecular-typing-and-antifungal-susceptibility-profile-of-candida-krusei-bloodstream-isolates-from-t%C3%A3-rkiye
#25
JOURNAL ARTICLE
Ayşe Sultan Karakoyun, Bram Spruijtenburg, Nevzat Unal, Eelco F J Meijer, Mete Sucu, Süleyha Hilmioğlu-Polat, Jacques F Meis, Theun de Groot, Macit Ilkit
Candida krusei also known as Pichia kudriavzevii is a potentially multidrug-resistant yeast because it is intrinsically resistant to fluconazole and develops acquired resistance to echinocandins and polyenes. Here, we aim to provide a better understanding of the epidemiology and transmission modes of C. krusei infections by comparing invasive bloodstream (n = 35) and non-invasive vaginal (n = 20) C. krusei isolates. The genetic relatedness of the isolates was assessed using a newly described short tandem repeat (STR) analysis and their sensitivity to eight antifungal compounds was evaluated by antifungal susceptibility testing using the CLSI microbroth dilution method...
January 30, 2024: Medical Mycology
https://read.qxmd.com/read/38285608/is-milbemycin-a-game-changer-against-antifungal-drug-resistant-mycoses
#26
JOURNAL ARTICLE
Rui Kano, Tsuyoshi Yamada, Takashi Tamura, Koichi Makimura
Milbemycin oximes are macrocyclic lactones that have a broad spectrum of activity against nematode infection in animals. They are known to block drug efflux, which increases the susceptibility of fungi to azoles. We investigated the effects of milbemycin on the azole susceptibility of fungi (Aspergillus fumigatus, Candida albicans, Candida auris, Cryptococcus neoformans, and Trichophyton rubrum). To screen for changes in azole susceptibility, fungal growth was tested on culture medium containing 1 μg/ml milbemycin...
January 29, 2024: Medical Mycology
https://read.qxmd.com/read/38242842/detection-of-scedosporium-spp-colonizer-or-pathogen-a-retrospective-analysis-of-clinical-significance-and-management-in-a-large-tertiary-centre
#27
JOURNAL ARTICLE
Ilana Reinhold, Chantal Quiblier, Frank Blaser, Jan Bögeholz, Frank Imkamp, Macé M Schuurmans, Michael B Soyka, Reinhard Zbinden, Nicolas J Mueller
Infections with Scedosporium spp. are emerging in the past two decades and are associated with a high mortality rate. Microbiological detection can be associated with either a colonization or infection. Evolution from colonization into infection is difficult to predict and clinical management upon microbiological detection is complex. Microbiological samples from 2015 to 2021 were retrospectively analyzed in a single tertiary care centre. Classification into colonization or infection was performed upon first microbiological detection...
January 18, 2024: Medical Mycology
https://read.qxmd.com/read/38228404/deep-cutaneous-mycoses-in-kidney-transplant-recipients-diagnostic-and-therapeutic-challenges
#28
JOURNAL ARTICLE
Chloé Bertin, Emilie Sitterlé, Anne Scemla, Sylvie Fraitag, Sarah Delliere, Sarah Guegan, Dea Garcia Hermoso, Stéphanie Leclerc-Mercier, Claire Rouzaud, Fanny Lanternier, Marie-Elisabeth Bougnoux
Deep cutaneous mycoses (DCMs) are rare infections that extend throughout the dermis and subcutis, often occurring after inoculation with pathogenic fungi. Trends toward a growing incidence have been observed that may be partially related to an increasing population of solid organ transplant patients. The aim of this study is to describe the diagnostic and the outcomes of DCM among kidney transplant recipients so as to optimize their management. We performed a retrospective review of cases of DCM occurring among kidney transplant recipients in our institution over 12 years...
January 16, 2024: Medical Mycology
https://read.qxmd.com/read/38196143/knowledge-and-regulation-on-fungal-contamination-of-sand-and-water-progress-report-and-perspectives
#29
JOURNAL ARTICLE
Jean-Pierre Gangneux, Joao Brandao, Ester Segal
Fungal flora in coastal/inland beach sand and recreational water is a neglected field of study, despite its potential impact on human health. A joint ISHAM/ECMM working group was formed in 2019 with the task to set up a vast international initiative aimed at studying the fungal contamination of beaches and bathing waters. Here we review the importance of the topic, and list the main results and achievements from 12 scientific publications. Fungal contamination exists at different levels and the genera most frequently found were Aspergillus spp...
January 9, 2024: Medical Mycology
https://read.qxmd.com/read/38171496/detection-of-blastomyces-gilchristii-via-metagenomic-sequencing-in-outbreak-associated-soils
#30
JOURNAL ARTICLE
Alexandra M Linz, Jennifer L Anderson, Jennifer K Meece
Cases of Blastomycosis, a serious fungal disease globally rare but endemic to North America, can appear both sporadically and in outbreaks. Tracing these outbreaks to their environment has traditionally used culturing and polymerase chain reaction (PCR). Here, we present our method for metagenomic detection of Blastomyces in a 2015 outbreak soil sample from central Wisconsin. By sequencing this sample to multiple depths, we simulated the minimum required depth to detect Blastomyces in this outbreak. Our methods and recommendations can be used to identify the sources of blastomycosis during outbreaks and to learn about the ecology of Blastomyces...
January 3, 2024: Medical Mycology
https://read.qxmd.com/read/38167789/evaluation-of-the-mannan-antigen-assay-in-neonates-with-or-without-candida-albicans-colonization
#31
JOURNAL ARTICLE
Vasiliki Bourika, Tania Siahanidou, Kalliopi Theodoridou, Athanasios Tsakris, Georgia Vrioni, Athanasios Michos
Mannan antigen (MA) in neonates as a marker of invasive candidemia is not well studied, although 4% of all neonatal intensive care unit admissions are attributed to Candida spp. infections. The aim of this case-control study was to evaluate the performance of MA(Platelia™ Candida AgPluskit, Bio-Rad) in neonates who had rectal Candida colonization or in non-colonized controls. We cultured 340 rectal swabs of neonates and MA was negative in 24/25 C. albicans colonized (96% sensitivity) and in 30/30 non-colonized neonates (100% specificity)...
January 2, 2024: Medical Mycology
https://read.qxmd.com/read/38518099/correction-to-neutrophil-extracellular-traps-nets-and-th-2-dominant-immune-responses-in-chronic-granulomatous-chromoblastomycosis
#32
(no author information available yet)
No abstract text is available yet for this article.
March 7, 2024: Medical Mycology
https://read.qxmd.com/read/38289831/performance-of-mucorales-spp-qpcr-in-bronchoalveolar-lavage-fluid-for-the-diagnosis-of-pulmonary-mucormycosis
#33
JOURNAL ARTICLE
Xavier Brousse, Sébastien Imbert, Nahéma Issa, Edouard Forcade, Maxime Faure, Jeremy Chambord, Hanta Ramaroson, Hannah Kaminski, Pierre-Yves Dumas, Elodie Blanchard
To estimate the diagnostic performance of Mucorales polymerase chain reaction (PCR) in Bronchoalveolar lavage fluid (BALF) in routine practice. This was a single-center retrospective study including all consecutive patients >18 years who underwent Mucorales PCR assay in BALF between January 2021 and May 2022. Index testing was prospectively performed using the MycoGENIE Aspergillus spp.-Mucorales spp. PCR. The reference was the diagnosis of pulmonary mucormycosis by the Adjudication Committee. Mucorales PCR in BALF was performed for 938 patients and was positive for 21 of 938 (2...
January 27, 2024: Medical Mycology
https://read.qxmd.com/read/38154488/the-nairobi-declaration-2023-a-commitment-to-address-deadly-yet-neglected-fungal-diseases-in-africa
#34
JOURNAL ARTICLE
Iriagbonse I Osaigbovo, Nelesh P Govender, Alexander M Jordan, Felix Bongomin, David B Meya, Alice Kanyua, Olga M Mashedi, David Koffi, Angela Loyse, Aude Sturny-Leclère, Jean-Pierre Gangneux, David W Denning, Tom Chiller, Oliver Cornely, Rita O Oladele
On May 30th and 31st, 2023, delegates representing various African subregions, together with global representatives from the International Society of Human and Animal Mycology (ISHAM), European Confederation of Medical Mycology (ECMM,) United States Centre for Disease Control and Prevention (CDC) and Global Action for Fungal Infections (GAFFI), convened in Nairobi, Kenya under the aegis of the Pan African Mycology Working Group, a working group of ISHAM. The meeting objectives were, amongst others, to deliberate on a continental response to the World Health Organization Fungal Priority Pathogen List and facilitate interaction between global and regional leaders...
December 28, 2023: Medical Mycology
https://read.qxmd.com/read/38148116/the-dog-as-a-sentinel-and-animal-model-for-coccidioidomycosis
#35
JOURNAL ARTICLE
Adam J Ferguson, George R Thompson, David Bruyette, Jane E Sykes
Coccidioidomycosis is a potentially fatal fungal disease of humans and animals that follows inhalation of Coccidioides spp. arthroconidia in the environment. The disease in dogs resembles that in people, and because dogs may be at increased risk of exposure due to their proximity to the ground and digging behavior, they are valuable models for the disease in humans. Dogs have been sentinels for identification of new regions of endemicity in Washington and Texas. Canine serosurveillance has also been used to predict variables associated with environmental presence of Coccidioides spp...
December 26, 2023: Medical Mycology
https://read.qxmd.com/read/38142226/nosocomial-transmission-of-aspergillus-flavus-in-a-neonatal-intensive-care-unit-long-term-persistence-in-environment-and-interest-of-maldi-tof-mass-spectrometry-coupled-with-convolutional-neural-network-cnn-for-rapid-clone-recognition
#36
JOURNAL ARTICLE
Noshine Mohammad, Huguenin Antoine, Annick Lefebvre, Laura Menvielle, Dominique Toubas, Stéphane Ranque, Isabelle Villena, Xavier Tannier, Anne-Cécile Normand, Renaud Piarroux
Aspergillosis of the new-born remains a rare but severe disease. We report four cases of primary cutaneous A. flavus infections in premature new-borns linked to incubators contamination by putative clonal strains. Our objective was to evaluate the ability of MALDI-TOF coupled to Convolutional Neural Network (CNN) for clone recognition in a context where only a very small number of strains are available for machine learning. Clinical and environmental A. flavus isolates (n = 64) were studied, 15 were epidemiologically related to the four cases...
December 23, 2023: Medical Mycology
https://read.qxmd.com/read/38130236/machine-learning-to-identify-clinically-relevant-candida-yeast-species
#37
JOURNAL ARTICLE
Shamanth A Shankarnarayan, Daniel A Charlebois
Fungal infections, especially due to Candida species, are on the rise. Multi-drug resistant organisms such as Candida auris are difficult and time consuming to identify accurately. Machine learning is increasingly being used in health care, especially in medical imaging. In this study, we evaluated the effectiveness of six convolutional neural networks (CNNs) to identify four clinically important Candida species. Wet-mounted images were captured using bright field live-cell microscopy followed by separating single cells, budding cells, and cell group images which were then subjected to different machine learning algorithms (custom CNN, VGG16, ResNet50, InceptionV3, EfficientNetB0, and EfficientNetB7) to learn and predict Candida species...
December 21, 2023: Medical Mycology
https://read.qxmd.com/read/38130212/predicting-intracranial-involvement-unveiling-perineural-spread-in-covid-19-associated-mucormycosis-a-novel-phenomenon
#38
JOURNAL ARTICLE
Nidhin Das K, Vidhu Sharma, Diksha Gupta, Sarbesh Tiwari, Poonam Elhence, Vidhi Jain, Kapil Soni, Amit Goyal
This study aimed to investigate the risk factors associated with intracranial involvement in COVID-19-associated mucormycosis (CAM) and to develop a nomogram model for predicting the risk of intracranial involvement, with a specific focus on perineural spread. An ambispective analysis was conducted on 275 CAM patients who received comprehensive treatment. Univariable and multivariable logistic regression analyses were performed to identify independent risk factors, and a nomogram was created based on the results of the multivariable analysis...
December 21, 2023: Medical Mycology
https://read.qxmd.com/read/38126122/the-incidences-and-clinical-outcomes-of-cryptococcosis-in-taiwan-a-nationwide-population-based-study-2002-2015
#39
JOURNAL ARTICLE
Shang-Ting Guan, Yu-Shan Huang, Shih-Tsung Huang, Fei-Yuan Hsiao, Yee-Chun Chen
Large-scale epidemiological data on cryptococcosis other than cryptococcal meningitis (CM), HIV- or solid organ transplantation (SOT)-associated cryptococcosis are limited. This study investigated the disease burden of cryptococcosis in Taiwan over 14 years. Incident episodes of cryptococcosis, comorbidities, treatment, and outcomes were captured from Taiwan's National Health Insurance Research Database and National Death Registry between 2002 and 2015. Of 6 647 episodes analyzed, the crude incidence rate per 100 000 population increased from 1...
December 20, 2023: Medical Mycology
https://read.qxmd.com/read/38066698/review-of-treatment-options-for-a-multi-drug-resistant-fungus-candida-auris
#40
JOURNAL ARTICLE
Siqi Wang, Jiangwei Pan, Liting Gu, Wei Wang, Bin Wei, Huawei Zhang, Jianwei Chen, Hong Wang
Candida auris is a widely distributed, highly lethal, multidrug-resistant fungal pathogen. It was first identified in 2009 when it was isolated from fluid drained from the external ear canal of a patient in Japan. Since then, it has caused infectious outbreaks in over 45 countries, with mortality rates approaching 60%. Drug resistance is common in this species, with a large proportion of isolates displaying fluconazole resistance and nearly half are resistant to two or more antifungal drugs. In this review, we describe the drug-resistance mechanism of C...
December 8, 2023: Medical Mycology
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