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Journal of Gene Medicine

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https://www.readbyqxmd.com/read/28744963/association-of-incretins-receptors-genetic-polymorphisms-with-type-2-diabetes-mellitus-in-egyptian-patients
#1
Sally M Shalaby, Haidy E Zidan, AmiraShokry, Jehan Saeed, Rehab H El-Sokkary
INTRODUCTION: Incretins opened a new era in T2DM pathogenesis. AIMS: To assess whether there is an association between GIPR rs2302382 and GIPR rs1800437 and GLP-1R rs367543060 polymorphisms with T2DM or not and also to determine the effect of these polymorphisms on GIP and GLP-1 levels. RESULTS: One hundred and fifty T2DM patients and 150 healthy controls were included in the study, polymorphisms of GIPR rs1800437, GIPR rs2302382 and GLP-1R rs367543060 were genotyped using RFLP-PCR, multiplex allele-specific PCR and RFLP-PCR respectively...
July 25, 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/28744947/bmp-2-gene-activated-muscle-tissue-fragments-for-osteochondral-defect-regeneration-in-the-rabbit-knee
#2
Volker M Betz, Alexander Keller, Peter Foehr, Christian Thirion, Michael Salomon, Stefan Rammelt, Hans Zwipp, Rainer Burgkart, Volkmar Jansson, Peter E Müller, Oliver B Betz
BACKGROUND: Previously published data indicate that BMP-2 gene activated muscle tissue grafts can repair large bone defects in rats. This innovative abbreviated ex vivo gene therapy is appealing since it does not require elaborative and time-consuming extraction and expansion of cells. Hence, in the here presented experimental study we evaluated the potential of this expedited tissue engineering approach to regenerate osteochondral defects in rabbits. METHODS: Autologous muscle tissue grafts from female White New Zealand rabbits were directly transduced with an adenoviral BMP-2 vector or remained unmodified...
July 25, 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/28730622/susceptibility-to-hiv-1-infection-is-influenced-by-toll-like-receptor-2-196-to-174-polymorphism-in-north-indian-population
#3
Sanjukta Vidyant, Animesh Chatterjee, Vikas Agarwal, Tapan N Dhole
INTRODUCTION: Toll like receptors (TLRs) are pattern recognition receptors, recognize molecular patterns of pathogens and play an important role in innate immunity. Recent studies have identified that single nucleotide polymorphism (SNPs) in TLR gene impairs the response to TLR ligands in some individuals and is associated with susceptibility to various infectious diseases. AIM: The aim of the present study is to investigate the role of four SNPs in the TLR2 gene, -196 to -174 Ins/Del, 2258 G/A (Arg753Gln), 2029 C/T (Arg677Trp) and 1892 C/A (Pro631His) with susceptibility and progression to HIV-1 in North Indian individuals...
July 20, 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/28688213/multivalent-methionine-functionalized-biocompatible-block-copolymers-for-targeted-sirna-delivery-and-subsequent-reversal-effect-on-adriamycin-resistance-in-human-breast-cancer-cell-line-mcf-7-adr
#4
Yang Wu, Wei Zhang, Tinghan Li, Rong Ma, Dan Chen, Junying Zhang, Jianzhong Wu, Jinhai Tang
BACKGROUND: Cationic polymers are the outstanding representatives of the most efficient siRNA vectors. Low cytotoxicity and siRNA protecting effect can be obtained with these cationic polymers via a variety of structural modifications. Nevertheless, the gap between their efficiency and the requirement for therapeutic processes is still noticeable. METHODS: A cationic polymer vector was synthesized via the copolymerization of N-(1, 3-dihydroxy propan-2-yl) methacrylamide (DHPMA) and N-(3-aminopropyl) methacrylamide (APMA)...
July 8, 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/28667693/in-vitro-and-in-vivo-gene-delivery-using-chitosan-hyaluronic-acid-nanoparticles-influences-of-molecular-mass-of-hyaluronic-acid-and-lyophilization-on-the-transfection-efficiency
#5
Toshinori Sato, Mitsuhiro Nakata, Zhihong Yang, Yu Torizuka, Satoko Kishimoto, Masayuki Ishihara
BACKGROUND: Lyophilization is an effective method for preserving non-viral gene vectors. To improve the stability and transgene expression of lyophilized plasmid DNA (pDNA) complexes, we coated the surfaces of pDNA/chitosan complexes with hyaluronic acid (HA) having varying molecular masses. The transgene expression of pDNA/chitosan/HA ternary complexes was characterized in vitro and in vivo. METHODS: pDNA complexes were lyophilized overnight and the resultant products with spongy, porous consistencies were stored at -30 °C, 4 °C, or 25 °C for two weeks...
July 1, 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/28658716/sustained-secretion-of-anti-tnf-alpha-monoclonal-antibody-from-ex-vivo-genetically-engineered-dermal-tissue-demonstrates-therapeutic-activity-in-mouse-model-of-rheumatoid-arthritis
#6
Inbal Zafir-Lavie, Reem Miari, Shay Sherbo, Simi Krispel, Osnat Tal, Atar Liran, Tamar Shatil, Felix Badinter, Haim Goltsman, Nir Shapir, Itai Benhar, Garry A Neil, Amos Panet
BACKGROUND: Rheumatoid arthritis is a symmetric inflammatory polyarthritis associated with high concentrations of pro-inflammatory cytokines including TNF-α. Adalimumab is a monoclonal antibody (mAb) that binds TNF-α, and is widely used to treat rheumatoid arthritis. Despite its proven clinical efficacy, adalimumab and other therapeutic mAbs have disadvantages including the requirement for repeated bolus injections and the appearance of treatment limiting anti-drug antibodies. To address these issues, we have developed an innovative ex-vivo gene therapy approach, Transduced Autologous Restorative Gene Therapy (TARGT), to produce and secrete adalimumab for the treatment of RA...
June 28, 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/28581127/correlation-analysis-of-hla-dpb1-05-01-gene-and-btnl2-gene-within-mhc-region-with-clinical-phenotype-of-psoriasis-vulgaris-in-the-chinese-han-population
#7
Huimin Guo, Yong Huang, Juan Wu, Xiaodong Zheng, Lei Ye, Hequn Huang, Wenjun Wang, Qi Zhen, Jing Wu, Wenjun Qian, Hui Cheng, Xing Fan, Xuejun Zhang
BACKGROUND: The human major histocompatibility complex (MHC) is well known to be highly polymorphic and has been identified to be associated with numerous diseases. HLA-DPB1 gene and BTNL2 gene were associated with psoriasis for the first time. This study aims to investigate the relevance of HLA-DPB1 gene and BTNL2 gene with clinical phenotypes of psoriasis vulgaris (PV). METHODS: To investigate the HLA-DPB1 gene and BTNL2 gene polymorphism which was association with clinical phenotypes of PV in Chinese Han population, we conducted an analysis in case-control and case-only subjects in the 9906 controls and 8744 cases by MHC targeted sequencing stratified analysis in this study...
June 5, 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/28749040/retraction-microrna-4656-is-a-prognostic-factor-and-tumor-suppressor-in-human-pancreatic-cancer-through-a-downstream-target-of-trka-by-xianglong-tan-jinyong-lv-guodong-zhao-zhiming-zhao-chenggang-li-yong-xu-and-minggen-hu
#8
https://www.readbyqxmd.com/read/28636766/association-of-tnfsf13-polymorphisms-with-iga-nephropathy-in-a-chinese-han-population
#9
Zhong Zhong, Shao-Zhen Feng, Ri-Cong Xu, Zhi-Jian Li, Feng-Xian Huang, Pei-Ran Yin, Wen-Ting Liu, Meng Wang, Dian-Chun Shi, Qian Zhou, Xue-Qing Yu, Ming Li
BACKGROUND: Our previous genome-wide association study of IgA nephropathy (IgAN) in a Chinese Han population suggested that the TNFSF13 gene may be a novel susceptibility gene for IgAN. In the present study, we aimed to further evaluate the associations of single-nucleotide polymorphisms (SNPs) and expression level of the TNFSF13 gene with the risk and clinical parameters of IgAN. METHODS: Six candidate SNPs were selected for genotyping by Sequenom MassARRAY iPLEX in 1000 IgAN cases and 1000 controls...
June 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/28632911/polymer-functionalized-gold-nanoparticles-as-nonviral-gene-delivery-reagents
#10
M Mar Encabo-Berzosa, Maria Sancho-Albero, Victor Sebastian, Silvia Irusta, Manuel Arruebo, Jesus Santamaria, Pilar Martín Duque
BACKGROUND: In the present study, we investigated the ability of polyethylene glycol (PEG) functionalized gold nanoparticles to function as nonviral vectors in the transfection of different cell lines, comparing them with commercial lipoplexes. METHODS: Positively-charged gold nanoparticles were synthesized using polyethylenimine (PEI) as a reducing and stabilizer agent and its cytotoxicity was reduced by its functionalization with PEG. We bound the nanoparticles to three plasmids with different sizes (4-40 kpb)...
June 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/28623876/developmental-history-and-application-of-crispr-in-human-disease
#11
REVIEW
Puping Liang, Xiya Zhang, Yuxi Chen, Junjiu Huang
Genome-editing tools are programmable artificial nucleases, mainly including zinc-finger nucleases, transcription activator-like effector nucleases and clustered regularly interspaced short palindromic repeat (CRISPR). By recognizing and cleaving specific DNA sequences, genome-editing tools make it possible to generate site-specific DNA double-strand breaks (DSBs) in the genome. DSBs will then be repaired by either error-prone nonhomologous end joining or high-fidelity homologous recombination mechanisms. Through these two different mechanisms, endogenous genes can be knocked out or precisely repaired/modified...
June 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/28591938/variants-in-mody-genes-associated-with-maternal-lipids-profiles-in-second-trimester-of-pregnancy
#12
Xiaojing Wang, Wei Li, Liangkun Ma, Fan Ping, Juntao Liu, Xueyan Wu, Jiangfeng Mao, Xi Wang, Min Nie
BACKGROUND: Dyslipidemia during pregnancy increases the risk of complications of pregnancy. Lipid profiles have a strong genetic determinant and numerous susceptibility loci have been identified. However, very few studies have focused on the association of lipid-related loci and maternal serum lipids during pregnancy. For the first time, we investigated the association of common variants in three maturity onset diabetes of the young (MODY) genes (HNF1A, HNF4A and HNF1B) with serum lipid concentrations and glucose metabolism related quantitative traits in the second trimester of pregnancy...
June 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/28544509/association-of-arhgap22-gene-polymorphisms-with-the-risk-of-type-2-diabetic-retinopathy
#13
Rong Li, Peng Chen, Jing Li, Mengdan Yan, Jingjie Li, Shanqu Li, Hongli Zhu
BACKGROUND: Little is known about the contribution of ARHGAP22 polymorphism to diabetic retinopathy (DR) risk. We performed a case-control study to investigate the associations between ARHGAP22 and the risk of DR in a Chinese Han population. METHODS: A total of 341 patients with type 2 diabetes mellitus (T2DM) were selected. All patients underwent a complete eye examination. Based on this, the patients with T2DM were divided into two subgroups: 188 patients with DR and 153 patients without DR...
June 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/27556228/microrna-144-regulates-cancer-cell-proliferation-and-cell-cycle-transition-in-acute-lymphoblastic-leukemia-through-the-interaction-of-fmn2
#14
Jie Jin, Yu Wang, Yang Xu, Xu Zhou, Yu Liu, Xiang Li, Jin Wang
BACKGROUND: In the present study, we explored the functional roles of microRNA-144 (miR-144) upregulation and downregulation in human acute lymphoblastic leukemia (ALL). METHODS: Gene expression of miR-144 was examined using the quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) in both ALL cell lines and T-leukemic cells of ALL patients. In ALL cell lines Molt-3 and Jurkat cells, miR-144 was either upregulated or downregulated through lentiviral transduction...
June 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/28474840/cox2-and-nos3-gene-polymorphisms-in-women-with-gestational-diabetes
#15
Maciej Tarnowski, Marta Tkacz, Violetta Dziedziejko, Krzysztof Safranow, Andrzej Pawlik
BACKGROUND: Gestational diabetes (GDM) is carbohydrate intolerance occurring in pregnancy. Low-grade inflammation plays an important role in the pathogenesis of this disorder. The aim of this study was to examine the association between COX2 (rs6681231) and NOS3 (rs1799983, rs2070744) gene polymorphisms and GDM. METHODS: This study included 204 pregnant women with GDM and 207 pregnant women with normal glucose tolerance (NGT). The diagnosis of GDM was based on a 75 g oral glucose tolerance test (OGTT) at 24-28 weeks' gestation...
May 5, 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/28544179/risk-of-high-altitude-pulmonary-edema-and-telomere-length
#16
LETTER
Gaurav Sikri, Srinivasa Bhattachar
No abstract text is available yet for this article.
May 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/28444911/response-to-the-letter-to-the-editor-by-d-yuan-risk-of-high-altitude-pulmonary-edema-and-telomere-length
#17
LETTER
Dongya Yuan
No abstract text is available yet for this article.
May 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/28423213/systemic-tumor-targeted-sodium-iodide-symporter-nis-gene-therapy-of-hepatocellular-carcinoma-mediated-by-b6-peptide-polyplexes
#18
Sarah Urnauer, Kathrin Klutz, Geoffrey K Grünwald, Stephan Morys, Nathalie Schwenk, Christian Zach, Franz-Josef Gildehaus, Wolfgang Rödl, Manfred Ogris, Ernst Wagner, Christine Spitzweg
BACKGROUND: Nonviral polymer-based gene transfer represents an adaptable system for tumor-targeted gene therapy because various design strategies of shuttle systems, together with the mechanistic concept of active tumor targeting, lead to improved gene delivery vectors resulting in higher tumor specificity, efficacy and safety. METHODS: Using the sodium iodide symporter (NIS) as a theranostic gene, nonviral gene delivery vehicles based on linear polyethylenimine (LPEI), polyethylene glycol (PEG) and coupled to the synthetic peptide B6 (LPEI-PEG-B6), which specifically binds to tumor cells, were investigated in a hepatocellular carcinoma xenograft model for tumor selectivity and transduction efficiency...
May 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/28387446/polymorphisms-in-the-snrpn-gene-are-associated-with-obesity-susceptibility-in-a-spanish-population
#19
David Albuquerque, Licínio Manco, Luz M González, Guillermo Gervasini, Goitzane Marcaida Benito, Juan R González, Raquel Rodríguez-López
BACKGROUND: SNRPN, which codes for the RNA-binding SmN protein, is a candidate gene for Prader-Willi syndrome. One characteristic of this neuroendocrine disorder is hyperphagia resulting in extreme obesity later in life. In the present study, we aimed to assess whether variability within this gene could be implicated in obesity susceptibility. METHODS: A case-control study was performed including 265 unrelated patients with nonsyndromic and early-onset severe obesity, belonging to high-risk obesity families from Spanish ancestry; 184 healthy control individuals were included representative of the same genetic background and sex-matched...
May 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/28370939/stent-mediated-gene-and-drug-delivery-for-cardiovascular-disease-and-cancer-a-brief-insight
#20
REVIEW
Akshaya Krishnagopal, Aakash Reddy, Dwaipayan Sen
This review concisely recapitulates the different existing modes of stent-mediated gene/drug delivery, their considerable advancement in clinical trials and a rationale for other merging new technologies such as nanotechnology and microRNA-based therapeutics, in addition to addressing the limitations in each of these perpetual stent platforms. Over the past decade, stent-mediated gene/drug delivery has materialized as a hopeful alternative for cardiovascular disease and cancer in contrast to routine conventional treatment modalities...
May 2017: Journal of Gene Medicine
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