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Journal of Gene Medicine

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https://www.readbyqxmd.com/read/29106062/co-delivery-of-hif-1%C3%AE-sirna-and-5-fluorouracil-to-overcome-drug-resistance-in-gastric-cancer-sgc-7901-cells
#1
Yunna Chen, Li Sun, Dongdong Guo, Ziteng Wu, Weidong Chen
BACKGROUND: Drug resistance cancer cells have become a major problem in chemotherapy. To solve this problem co-delivery of siRNA and 5-fluorouracil chitosan nanoparticles were prepared to reverse the multidrug resistance of gastric cancer SGC-7901 cells in vitro. METHODS: Chitosan nanoparticles were prepared by ionic gel method. siRNA nanoparticles were characterized by gel retardation assays, particle size and zeta potential were measured to confirm nanoparticles formation...
November 6, 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/29076194/genetic-factors-are-stressed-variably-by-onset-age-based-sample-selection-in-psoriasis-a-hint-from-mhc-region-based-analysis
#2
Lei Ye, Chao Yang, Jin-Fa Dou, Lei-Lei Wen, Wen-Jun Wang, Xiao-Dong Zheng, Xian-Bo Zuo, Fu-Sheng Zhou, Xing Fan, Xue-Jun Zhang
BACKGROUND: Large cohort-based genetic association studies have been established over a decade. However for certain disease, different results in GWAS level were concluded among studies, even in the same ethnics. We hypothesized onset-age based sample variables might have great impacts on the results. METHODS: In this study, we divided psoriasis patients into several subgroups according to the onset age bracket. We conducted genetic association analysis in MHC region of each patient subgroup with shared control subjects...
October 26, 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/29059509/gene-therapy-for-neuropathic-pain-induced-by-spared-nerve-injury-with-naked-plasmid-encoding-hepatocyte-growth-factor
#3
Hu Chunsheng, Lu Yuxin, Cheng Xiaochen, Cui Yufang, Wu Zuze, Zhang Qinglin
BACKGROUND: Neuropathic pain (NP) is a refractory disease in clinic and tremendously impacts on the patients' quality of life. Gene therapy is a potential strategy for the management of NP. In this paper, we examined the analgesic effect and mechanism of hepatocyte growth factor (HGF) in vitro and in vivo. METHODS: We examined the proinflammatroy gene changes in lipopolysaccharide (LPS)-induced BV2 cells with quantitative real-time polymerase chain reaction (qRT-PCR) of interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α, and inducible nitric oxide synthase (iNOS)...
October 23, 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/29047186/il-1rn-gene-polymorphisms-were-associated-with-breast-cancer-risk-in-a-chinese-han-population
#4
Tianbo Jin, Wei Cao, Xiaoxiao Zuo, Miao Li, Ya Yang, Tiansong Liang, Hongyao Yang, Xinhan Zhao, Daoke Yang
BACKGROUND: Interleukin (IL) is a large family of endogenous cytokine which are crucial in the regulation of inflammation and immunological responses. IL-1 receptor antagonist (IL-1RN) has been found to be associated with risk breast cancer (BC) in Korean and Indian women. However, little information is found about the polymorphisms of IL-1RN in Chinese Han BC patient. METHODS: We investigated the association between single-nucleotide polymorphisms (SNPs) in IL-1RN and BC risk in a case-control study that included 530 BC cases and 628 healthy controls...
October 18, 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/29044877/sustained-fxn-expression-in-dorsal-root-ganglia-from-a-nonreplicative-genomic-hsv-1-vector
#5
Maria Ventosa, Zetang Wu, Filip Lim
BACKGROUND: Friedreich's ataxia (FA) is an autosomal recessive neurodegenerative disease caused by mutations in the frataxin gene (FXN), which lead to reduced levels of the essential mitochondrial protein frataxin. Currently there is no effective cure. METHODS: With the aim of developing a gene therapy for FA neuropathology, here we describe the construction and preliminary characterization of a high capacity nonreplicative genomic herpes simplex virus type 1 vector (H24B-FXNlac vector) carrying a reduced version of the human FXN genomic locus, comprising the 5 kb promoter and the FXN cDNA with the inclusion of intron 1...
October 17, 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/29044850/mesenchymal-stem-cells-therapy-a-promising-cell-based-therapy-for-treatment-of-myocardial-infraction
#6
REVIEW
Ayman El Sayed Shafei, Mahmoud Ahmed Ali, Hazem G Ghanem, Ahmed I Shehata, Ahmed A Abdelgawad, Hossam R Handal, Kareem A Talaat, Ahmed E Ashaal, Amal S El-Shal
AIM: For decades, mesenchymal stem (MSCs) cells had been used for cardiovascular diseases as regenerative therapy. This review is an attempt to summarize the types of MSCs involved in MI therapy, its possible mechanisms effects especially paracrine one in MI focusing on the studies (human and animal) conducted in the last 10 years. DISCUSSION: Recently, reports showed that MSC therapy could have infarct-limiting effects after myocardial infarction (MI) in both experimental and clinical trials...
October 16, 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/29024250/non-invasive-detection-of-bladder-cancer-via-expression-targeted-gene-delivery
#7
Yunlan Fang, B Wolfson, W T Godbey
BACKGROUND: Because of the time and expense associated with the procedures and possible distress to the patient, cystoscopy or other imaging techniques are typically not used for bladder cancer detection before symptoms become present. Alternatively, commercial assays for urinary tumor markers exist but are marred by low sensitivity and high cost. There is a need for a simple and sensitive means of tumor detection, such as via the analysis of urine. METHODS: Plasmids encoding the secretable reporter Gaussia luciferase (G...
October 11, 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/28990350/association-of-dnmt3b-gene-variants-with-sporadic-parkinson-s-disease-in-a-chinese-han-population
#8
Xiang Chen, Yousheng Xiao, Lei Wei, Yijuan Wu, Jianjun Lu, Wenyuan Guo, Shuxuan Huang, Miaomiao Zhou, Mingshu Mo, Zhe Li, Luan Cen, Shaomin Li, ChaohaoYang, Zhuohua Wu, Sophie Hu, Zhong Pei, XinLing Yang, Shaogang Qu, Pingyi Xu
INTRODUCTION: Parkinson's disease (PD) is the second most common neurodegenerative disorder worldwide. Epigenetic modifications, specifically DNA methylation, have been implicated in the development of this disease. Genetic variants of DNA methyltransferase 3b (DNMT3b), one of the most important DNA methyltransferases, were shown to be associated with PD in a Brazilian population. However, it is unclear whether genetic variants of DNMT3b increase the risk of PD in the Chinese Han people...
October 9, 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/28990296/association-between-mthfr-microrna-binding-site-polymorphisms-and-methotrexate-concentrations-in-chinese-pediatric-patients-with-acute-lymphoblastic-leukemia
#9
Shu-Mei Wang, Wei-Xin Zeng, Wan-Shui Wu, Lu-Lu Sun, Dan Yan
BACKGROUND: The pharmacokinetics and therapeutic response to methotrexate (MTX) display large variability in the treatment of acute lymphoblastic leukemia (ALL). The aim of the present study was to investigate the association of two microRNA (miRNA) binding site polymorphisms (rs3737966 G > A and rs35134728 DEL/TTC) in the 3'UTR of MTHFR with serum MTX concentrations, in a Chinese pediatric population with ALL. METHODS: Genotyping for MTHFR rs3737966 and rs35134728 in 144 children with ALL was performed using the Sequenom MassArray system...
October 9, 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/28985021/the-effects-of-gene-polymorphisms-on-glioma-prognosis
#10
Ying Cui, Guolin Li, Mengdan Yan, Jing Li, Tianbo Jin, Shanqu Li, Shijie Mu
PURPOSE: Malignant gliomas are the most common primary brain tumors. Various genetic factors play important roles in the development and prognosis of glioma. This study focuses on the impact of MPHOSPH6, TNIP1, and several other genes (ACYP2, NAF1, TERC, TERT, OBFC1, ZNF208, and RTEL1) on telomere length and how that affects the prognosis of glioma. MATERIALS AND METHODS: Forty-three polymorphisms in nine genes from 605 glioma patients were selected. The association between genotype and survival outcome was analyzed using the Kaplan-Meier method, Cox regression analysis, and the log-rank test...
October 6, 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/29076590/erratum-posterior-tibial-tendinopathy-associated-with-matrix-metalloproteinase-13-promoter-genotype-and-haplotype
#11
(no author information available yet)
No abstract text is available yet for this article.
September 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/28940887/association-of-human-leukocyte-antigen-polymorphisms-with-occult-hepatitis-b-virus-infection-in-a-shaanxi-han-population
#12
Tianju Wang, Chunmei Shen, Liping Chen, Sheng Liu, Yanhong Ji
BACKGROUND: Occult hepatitis B virus (HBV) infection (OBI) is defined as HBV DNA detection in serum or in the liver by sensitive diagnostic tests in HBV surface antigen (HBsAg) negative patients with or without serologic markers of previous HBV exposure. Because the human leukocyte antigen (HLA) system is an integral component of the immune response, we hypothesized that the highly polymorphic HLA genes were the key determinants of HBV persistence and clearance. The present study aimed to calculate the allelic frequency of HLA loci and investigate the association between HLA alleles and the outcome of OBI in Shaanxi Han population in the northwest of China...
September 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/28940489/polymorphisms-in-homologous-recombination-repair-genes-and-the-risk-and-survival-of-breast-cancer
#13
Yu-Huang Liao, Jun-Ting Ren, Wei Zhang, Zheng-Zheng Zhang, Ying Lin, Feng-Xi Su, Wei-Hua Jia, Lu-Ying Tang, Ze-Fang Ren
BACKGROUND: Immunoglobulin (Ig)A antibody of Epstein-Barr virus (EBV) was found to associate with breast cancer (BC), whereas IgA positivity was related to a series of genetic markers in the genes of homologous recombination repair system (HRRs). We assessed the associations of the polymorphisms in HRR genes with the risk and survival of BC. METHODS: A case-control study was conducted with 1551 bc cases and 1605 age-matched healthy controls between October 2008 and March 2012 in the Guangzhou Breast Cancer Study (GZBCS), China, and the case population were followed up until 31 January 2016...
September 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/28744963/association-of-incretin-receptors-genetic-polymorphisms-with-type-2-diabetes-mellitus-in-egyptian-patients
#14
Sally M Shalaby, Haidy E Zidan, Amira Shokry, Jehan Saeed, Rehab H El-Sokkary
BACKGROUND: Incretins have opened a new era in type 2 diabetes mellitus (T2DM) pathogenesis. The present study aimed to assess whether there is an association between GIPR rs2302382, GIPR rs1800437 and GLP-1R rs367543060 polymorphisms with T2DM or not and also to determine the effect of these polymorphisms on gastric inhibitory polypeptide (GIP) and glucagon-like peptide-1 (GLP-1) levels. METHODS: One hundred and fifty T2DM patients and 150 healthy controls were included in the study...
September 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/28744947/bmp-2-gene-activated-muscle-tissue-fragments-for-osteochondral-defect-regeneration-in-the-rabbit-knee
#15
Volker M Betz, Alexander Keller, Peter Foehr, Christian Thirion, Michael Salomon, Stefan Rammelt, Hans Zwipp, Rainer Burgkart, Volkmar Jansson, Peter E Müller, Oliver B Betz
BACKGROUND: Previously published data indicate that BMP-2 gene activated muscle tissue grafts can repair large bone defects in rats. This innovative abbreviated ex vivo gene therapy is appealing because it does not require elaborative and time-consuming extraction and expansion of cells. Hence, in the present study, we evaluated the potential of this expedited tissue engineering approach for regenerating osteochondral defects in rabbits. METHODS: Autologous muscle tissue grafts from female White New Zealand rabbits were directly transduced with an adenoviral BMP-2 vector or remained unmodified...
September 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/28581127/correlation-analysis-of-the-hla-dpb1-05-01-and-btnl2-genes-within-the-histocompatibility-complex-region-with-a-clinical-phenotype-of-psoriasis-vulgaris-in-the-chinese-han-population
#16
Huimin Guo, Yong Huang, Juan Wu, Xiaodong Zheng, Lei Ye, Hequn Huang, Wenjun Wang, Qi Zhen, Jing Wu, Wenjun Qian, Hui Cheng, Xing Fan, Xuejun Zhang
BACKGROUND: The human major histocompatibility complex (MHC) is known to be highly polymorphic and has been identified to be associated with numerous diseases. The HLA-DPB1 and BTNL2 genes were associated with psoriasis for the first time. The present study aims to investigate the relevance of the HLA-DPB1 and BTNL2 genes with respect to clinical phenotypes of psoriasis vulgaris (PV). METHODS: To investigate whether the HLA-DPB1 and BTNL2 polymorphisms were associated with clinical phenotypes of PV in Chinese Han population, we conducted an analysis in case-controls and case-only subjects (9906 controls and 8744 cases) via MHC targeted sequencing stratified analysis...
September 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/28730622/susceptibility-to-hiv-1-infection-is-influenced-by-toll-like-receptor-2-196-to-174-polymorphism-in-north-indian-population
#17
Sanjukta Vidyant, Animesh Chatterjee, Vikas Agarwal, Tapan N Dhole
INTRODUCTION: Toll like receptors (TLRs) are pattern recognition receptors, recognize molecular patterns of pathogens and play an important role in innate immunity. Recent studies have identified that single nucleotide polymorphism (SNPs) in TLR gene impairs the response to TLR ligands in some individuals and is associated with susceptibility to various infectious diseases. AIM: The aim of the present study is to investigate the role of four SNPs in the TLR2 gene, -196 to -174 Ins/Del, 2258 G/A (Arg753Gln), 2029 C/T (Arg677Trp) and 1892 C/A (Pro631His) with susceptibility and progression to HIV-1 in North Indian individuals...
July 20, 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/28688213/multivalent-methionine-functionalized-biocompatible-block-copolymers-for-targeted-sirna-delivery-and-subsequent-reversal-effect-on-adriamycin-resistance-in-human-breast-cancer-cell-line-mcf-7-adr
#18
Yang Wu, Wei Zhang, Tinghan Li, Rong Ma, Dan Chen, Junying Zhang, Jianzhong Wu, Jinhai Tang
BACKGROUND: Cationic polymers are the outstanding representatives of the most efficient siRNA vectors. Low cytotoxicity and siRNA protecting effect can be obtained with these cationic polymers via a variety of structural modifications. Nevertheless, the gap between their efficiency and the requirement for therapeutic processes is still noticeable. METHODS: A cationic polymer vector was synthesized via the copolymerization of N-(1, 3-dihydroxy propan-2-yl) methacrylamide (DHPMA) and N-(3-aminopropyl) methacrylamide (APMA)...
July 8, 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/28667693/in-vitro-and-in-vivo-gene-delivery-using-chitosan-hyaluronic-acid-nanoparticles-influences-of-molecular-mass-of-hyaluronic-acid-and-lyophilization-on-the-transfection-efficiency
#19
Toshinori Sato, Mitsuhiro Nakata, Zhihong Yang, Yu Torizuka, Satoko Kishimoto, Masayuki Ishihara
BACKGROUND: Lyophilization is an effective method for preserving non-viral gene vectors. To improve the stability and transgene expression of lyophilized plasmid DNA (pDNA) complexes, we coated the surfaces of pDNA/chitosan complexes with hyaluronic acid (HA) having varying molecular masses. The transgene expression of pDNA/chitosan/HA ternary complexes was characterized in vitro and in vivo. METHODS: pDNA complexes were lyophilized overnight and the resultant products with spongy, porous consistencies were stored at -30 °C, 4 °C, or 25 °C for two weeks...
July 1, 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/28658716/sustained-secretion-of-anti-tnf-alpha-monoclonal-antibody-from-ex-vivo-genetically-engineered-dermal-tissue-demonstrates-therapeutic-activity-in-mouse-model-of-rheumatoid-arthritis
#20
Inbal Zafir-Lavie, Reem Miari, Shay Sherbo, Simi Krispel, Osnat Tal, Atar Liran, Tamar Shatil, Felix Badinter, Haim Goltsman, Nir Shapir, Itai Benhar, Garry A Neil, Amos Panet
BACKGROUND: Rheumatoid arthritis is a symmetric inflammatory polyarthritis associated with high concentrations of pro-inflammatory cytokines including TNF-α. Adalimumab is a monoclonal antibody (mAb) that binds TNF-α, and is widely used to treat rheumatoid arthritis. Despite its proven clinical efficacy, adalimumab and other therapeutic mAbs have disadvantages including the requirement for repeated bolus injections and the appearance of treatment limiting anti-drug antibodies. To address these issues, we have developed an innovative ex-vivo gene therapy approach, Transduced Autologous Restorative Gene Therapy (TARGT), to produce and secrete adalimumab for the treatment of RA...
June 28, 2017: Journal of Gene Medicine
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