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Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology

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https://www.readbyqxmd.com/read/29783147/analytical-performance-of-the-hologic-aptima-hbv-quant-assay-and-the-cobas-ampliprep-cobas-taqman-hbv-test-v2-0-for-the-quantification-of-hbv-dna-in-plasma-samples
#1
Kristian Schønning, Kim Johansen, Lone Gilmor Nielsen, Nina Weis, Henrik Westh
BACKGROUND: Quantification of HBV DNA is used for initiating and monitoring antiviral treatment. Analytical test performance consequently impacts treatment decisions. OBJECTIVES: To compare the analytical performance of the Aptima HBV Quant Assay (Aptima) and the COBAS Ampliprep/COBAS TaqMan HBV Test v2.0 (CAPCTMv2) for the quantification of HBV DNA in plasma samples. STUDY DESIGN: The performance of the two tests was compared on 129 prospective plasma samples, and on 63 archived plasma samples of which 53 were genotyped...
May 18, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29759805/corrigendum-to-reactivation-of-bk-polyomavirus-during-pregnancy-vertical-transmission-and-clinical-significance-a-meta-analysis-j-clin-virol-102-2018-56-62
#2
Wisit Cheungpasitporn, Charat Thongprayoon, Iasmina M Craici, Konika Sharma, Supavit Chesdachai, Nadeen J Khoury, Amy S Ettore
No abstract text is available yet for this article.
May 11, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29753103/genetic-diversity-of-human-sapovirus-across-the-americas
#3
Marta Diez-Valcarce, Christina J Castro, Rachel L Marine, Natasha Halasa, Holger Mayta, Mayuko Saito, Laura Tsaknaridis, Chao-Yang Pan, Filemon Bucardo, Sylvia Becker-Dreps, Maria Renee Lopez, Laura Cristal Magaña, Terry Fei Fan Ng, Jan Vinjé
BACKGROUND: Sapoviruses are responsible for sporadic and epidemic acute gastroenteritis worldwide. Sapovirus typing protocols have a success rate as low as 43% and relatively few complete sapovirus genome sequences are available to improve current typing protocols. OBJECTIVE/STUDY DESIGN: To increase the number of complete sapovirus genomes to better understand the molecular epidemiology of human sapovirus and to improve the success rate of current sapovirus typing methods, we used deep metagenomics shotgun sequencing to obtain the complete genomes of 68 sapovirus samples from four different countries across the Americas (Guatemala, Nicaragua, Peru and the US)...
May 6, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29763837/different-meteorological-parameters-influence-metapneumovirus-and-respiratory-syncytial-virus-activity
#4
Magali Darniot, Cécile Pitoiset, Laurine Millière, Ludwig Serge Aho-Glélé, Emmanuel Florentin, Jean-Baptiste Bour, Catherine Manoha
BACKGROUND: Both human metapneumovirus (hMPV) and respiratory syncytial virus (RSV) cause epidemics during the cold season in temperate climates. OBJECTIVES: The purpose of this study was to find out whether climatic factors are associated with RSV and hMPV epidemics. STUDY DESIGN: Our study was based on data from 4300 patients admitted to the Dijon University Hospital for acute respiratory infection (ARI) over three winter seasons chosen for their dissimilar meteorological and virological patterns...
May 5, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29753934/recovery-of-cytomegalovirus-dna-from-newborn-saliva-samples-by-different-methods
#5
Oran Goshen, David M Goldfarb, Laura Book, Peter Tilley, Soren Gantt
BACKGROUND: Polymerase chain reaction (PCR) testing of saliva swabs has been shown to be an attractive method to screen for congenital cytomegalovirus (CMV) infection. However, few data are available regarding the impact of pre-analytic methods on CMV DNA recovery from saliva samples under various conditions. OBJECTIVES: The purpose of this study was to evaluate the impact of various transport media and transport conditions on the recovery of CMV DNA from spiked and clinical saliva samples from newborns using PCR testing...
May 5, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29738895/clinical-and-biological-features-of-enteroviral-meningitis-among-adults-and-children-and-factors-associated-with-severity-and-length-of-stay
#6
Mo Vareil, H Wille, S Kassab, C Le-Cornec, M Puges, A Desclaux, M E Lafon, C Tumiotto, C Cazanave, D Neau
BACKGROUND: Enterovirus (EV) meningitis is the most common form of meningitis. Clinical and biological manifestations may be non-specific, leading to prolonged and costly investigations. OBJECTIVES: To determine the different aspects of EV meningitis and the variables associated with length of stay (LOS) in hospital independently of patients' age. STUDY DESIGN: Single center retrospective study of all EV PCR positive CSF samples during 3.5 years in Bordeaux University Hospital, France...
May 1, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29729547/the-risk-of-herpes-simplex-virus-and-human-cytomegalovirus-infection-during-pregnancy-upon-adverse-pregnancy-outcomes-a-meta-analysis
#7
REVIEW
Ting-Li Shi, Li-Ju Huang, Yi-Quan Xiong, Yan-Yun Zhong, Jin-Jun Yang, Ting Fu, Xie-Fen Lei, Qing Chen
BACKGROUND AND OBJECTIVES: Herpes simplex virus (HSV) and human cytomegalovirus (HCMV) are widespread infections in humans, yet their impact on adverse pregnancy outcomes is controversial. The objective of this study was to evaluate the impact of HSV and HCMV infections during pregnancy on adverse pregnancy outcomes. METHODS: A systematic literature search was performed using Web of Science, Scopus, Medline, Embase, PubMed, and the Cochrane Library database for relevant publications up to 2nd August 2017...
April 25, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29727833/cytomegalovirus-sequence-variability-amplicon-length-and-dnase-sensitive-non-encapsidated-genomes-are-obstacles-to-standardization-and-commutability-of-plasma-viral-load-results
#8
Klaudia Naegele, Irmeli Lautenschlager, Rainer Gosert, Raisa Loginov, Katia Bir, Ilkka Helanterä, Stefan Schaub, Nina Khanna, Hans H Hirsch
BACKGROUND: Cytomegalovirus (CMV) management post-transplantation relies on quantification in blood, but inter-laboratory and inter-assay variability impairs commutability. An international multicenter study demonstrated that variability is mitigated by standardizing plasma volumes, automating DNA extraction and amplification, and calibration to the 1st-CMV-WHO-International-Standard as in the FDA-approved Roche-CAP/CTM-CMV. However, Roche-CAP/CTM-CMV showed under-quantification and false-negative results in a quality assurance program (UK-NEQAS-2014)...
April 22, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29705613/false-human-cytomegalovirus-igg-positivity-at-prenatal-screening
#9
Milena Furione, Antonella Sarasini, Alessia Arossa, Chiara Fornara, Daniele Lilleri, Laurent Perez, Maurizio Parea, Maurizio Zavattoni, Arsenio Spinillo, Piero Marone, Fausto Baldanti
BACKGROUND: An incorrect definition of immune status to human cytomegalovirus (HCMV) can lead to incorrect management of pregnant women. OBJECTIVES: Aims of the study were: i) to describe 10 cases of unconfirmed HCMV IgG-seroconversion in pregnancy; ii) to develop a panel of confirmatory tests to define HCMV serostatus; iii) to investigate the frequency of false IgG-positive results in pregnant women screened with the LIAISON® CMVIgGII automated assay. STUDY DESIGN: Blood samples from 10 pregnant women referred for HCMV IgG-seroconversion were examined to confirm/exclude a primary infection...
April 21, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29704734/molecular-epidemiology-of-human-adenovirus-infections-in-denmark-2011-2016
#10
Céline Barnadas, Dennis Jelsbak Schmidt, Thea K Fischer, Jannik Fonager
BACKGROUND: Human adenoviruses (HAdVs) can cause respiratory tract infections, conjunctivitis, diarrhoea and outbreaks have been reported. However, little is known about the disease burden and the molecular epidemiology of HAdV. OBJECTIVES: To retrospectively perform a molecular characterization of HAdV positive samples received at Statens Serum Institut during the period 2011-2016 and to compare this with demographic information, geographic location, sample collection date and type and co-infection with other viral pathogens...
April 21, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29702351/hepatitis-e-virus-infection-in-different-groups-of-estonian-patients-and-people-who-inject-drugs
#11
Tatiana V Kuznetsova, Anna Ivanova-Pozdejeva, Irina Reshetnjak, Julia Geller, Kairi Värv, Reet Rumvolt, Maria Vikentjeva, Elena V Trubnikova, Natalia V Pozdniakova, Alexei B Shevelev, Irina Golovljova
BACKGROUND: Previously we demonstrated a high prevalence of hepatitis E virus (HEV) in domestic pigs and wild boars, the main reservoir and possible source of HEV infections in humans. But so far there are no reports about spread of HEV in Estonian human population. OBJECTIVES: The present study aimed to determine the prevalence and genotyping of HEV in different groups of the Estonian adult population. STUDY DESIGN: Totally 1426 human serum samples were tested (763 patients with clinically diagnosed nonA/B/C hepatitis, 176 hemodialysis patients, 282 patients with suspected HEV infection and 205 people who injected drugs (PWID))...
April 20, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29704736/viral-bronchiolitis-management-in-hospitals-in-the-uk
#12
Elliott J Carande, Eva P Galiza, Alecia Nickless, Andrew J Pollard, Simon B Drysdale
BACKGROUND: Viral bronchiolitis is the leading cause of hospitalisation in infants less than a year old. The United Kingdom (UK) National Institute for Health and Care Excellence (NICE) published a guideline for the management of viral bronchiolitis in June 2015. OBJECTIVES: This study aimed to prospectively survey the management of viral bronchiolitis in hospital Trusts in the UK to provide a baseline of practice prior to the publication of the 2015 NICE bronchiolitis guideline against which future practice can be assessed...
April 18, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29704735/heterophilic-interference-in-specimens-yielding-false-reactive-results-on-the-abbott-4th-generation-architect-hiv-ag-ab-combo-assay
#13
S Lavoie, D Caswell, M J Gill, K Kadkhoda, C L Charlton, P N Levett, T Hatchette, R Garceau, J Maregmen, T Mazzulli, R Needle, K Kadivar, J Kim
BACKGROUND: False-reactivity in HIV-negative specimens has been detected in HIV fourth-generation antigen/antibody or 'combo' assays which are able to detect both anti-HIV-1/HIV-2 antibodies and HIV-1 antigen. OBJECTIVES: We sought to characterize these specimens and determine the effect of heterophilic interference. STUDY DESIGN: Specimens previously testing as false-reactive on the Abbott ARCHITECT HIV Ag/Ab combo assay and re-tested on a different (Siemens ADVIA Centaur HIV Ag/Ab) assay...
April 12, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29674183/pnpla3-rs738409-polymorphism-is-associated-with-liver-fibrosis-progression-in-patients-with-chronic-hepatitis-c-a-repeated-measures-study
#14
María Ángeles Jiménez-Sousa, Ana Zaida Gómez-Moreno, Daniel Pineda-Tenor, Juan José Sánchez-Ruano, Amanda Fernández-Rodríguez, Tomas Artaza-Varasa, Alicia Gómez-Sanz, María Martín-Vicente, Sonia Vázquez-Morón, Salvador Resino
BACKGROUND: Host genetic background has been associated with liver fibrosis progression. OBJECTIVE: To analyze the association between the patatin-like phospholipase domain-containing 3 (PNPLA3) rs738409 polymorphism and liver fibrosis progression in hepatitis C virus (HCV)-infected patients. STUDY DESIGN: In this retrospective cohort study, 187 patients with chronic HCV infection were included, who had at least two liver stiffness measurements (LSM) by transient elastography during the follow-up...
April 12, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29704733/evaluation-of-the-national-laboratory-based-surveillance-system-for-respiratory-syncytial-virus-in-sweden-2015-2016
#15
Heli Harvala, AnnaSara Carnahan, Sarah Axelsson, Maria Brytting
BACKGROUND: Most laboratories use RSV PCR but near-patient tests (NPT) performed at paediatric clinics are believed to be increasingly used. Anonymised data on RSV infections has been collected since 1990 in Sweden. No evaluation of Swedish RSV surveillance or use of laboratory testing had previously been performed. OBJECTIVES: Swedish RSV data and methods used for RSV laboratory testing and reporting were evaluated in order to improve RSV surveillance in a forthcoming vaccine era...
April 10, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29702350/evaluation-of-rida-%C3%A2-gene-norovirus-gi-gii-real-time-rt-pcr-using-stool-specimens-collected-from-children-and-adults-with-acute-gastroenteritis
#16
N Kanwar, F Hassan, L Barclay, C Langley, J Vinjé, P W Bryant, K St George, L Mosher, J M Matthews-Greer, M A Rocha, D O Beenhouwer, C J Harrison, M Moffatt, N Shastri, R Selvarangan
BACKGROUND: Norovirus is the leading cause of epidemic and sporadic acute gastroenteritis (AGE) in the United States. Widespread prevalence necessitates implementation of accurate norovirus detection assays in clinical diagnostic laboratories. OBJECTIVE: To evaluate RIDA® GENE norovirus GI/GII real-time RT-PCR assay (RGN RT-PCR) using stool samples from patients with sporadic AGE. STUDY DESIGN: Patients between 14 days to 101 years of age with symptoms of AGE were enrolled prospectively at four sites across the United States during 2014-2015...
April 10, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29698873/clinical-significance-of-igm-and-iga-class-anti-nmdar-antibodies-in-herpes-simplex-encephalitis
#17
Gabriel Westman, Azita Sohrabian, Elisabeth Aurelius, Clas Ahlm, Silvia Schliamser, Fredrik Sund, Marie Studahl, Johan Rönnelid
BACKGROUND: Herpes simplex encephalitis (HSE) is a devastating disease, often leaving patients with severe disabilities. It has been shown that IgG anti-N-methyl-d-aspartate receptor (NMDAR) antibodies appear in approximately 25% of HSE patients and could be associated with impaired recovery of cognitive performance. OBJECTIVES: To characterize the prevalence of IgM and IgA anti-NMDAR antibodies in HSE patients, in relation to subsequent development of IgG anti-NMDAR and correlation to cognitive performance...
April 10, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29656087/molecular-features-of-hepatitis-e-virus-circulation-in-environmental-and-human-samples
#18
H Fenaux, M Chassaing, S Berger, H Jeulin, A Gentilhomme, M Bensenane, J P Bronowicki, C Gantzer, I Bertrand, E Schvoerer
BACKGROUND AND OBJECTIVES: Hepatitis E virus (HEV) is emerging but its circulation between humans and the environment remains misunderstood. HEV ORF2 gene encodes the capsid playing a key role in viral interactions with surfaces, ORF3 products are involved in the viral cycle. Our aim was to study the molecular characteristics of ORF2 and ORF3 which could favor HEV fitness in patients and the environment. STUDY DESIGN: Samples from 69 patients with hepatitis (blood/stools), 20 urban wastewaters, 20 effluents of a pig slaughterhouse, 22 farm pigs (stools), 20 wild boars (liver/stools) were collected in North-Eastern France...
April 5, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29655170/frequency-of-hepatitis-b-surface-antigen-variants-hbsag-in-hepatitis-b-virus-genotype-b-and-c-infected-east-and-southeast-asian-patients-detection-by-the-elecsys-%C3%A2-hbsag-ii-assay
#19
Hyon Suk Kim, Xinyue Chen, Min Xu, Cunling Yan, Yali Liu, Haohui Deng, Bui Huu Hoang, Pham Thi Thu Thuy, Terry Wang, Yiwen Yan, Zhen Zeng, Mikael Gencay, Gaston Westergaard, Stephan Pabinger, Albert Kriegner, Markus Nauck, Anja Seffner, Peter Gohl, Kirsten Hübner, Wolfgang E Kaminski
BACKGROUND: To avoid false negative results, hepatitis B surface antigen (HBsAg) assays need to detect samples with mutations in the immunodominant 'a' determinant region, which vary by ethnographic region. OBJECTIVE: We evaluated the prevalence and type of HBsAg mutations in a hepatitis B virus (HBV)-infected East- and Southeast Asian population, and the diagnostic performance of the Elecsys® HBsAg II Qualitative assay. STUDY DESIGN: We analyzed 898 samples from patients with HBV infection from four sites (China [Beijing and Guangzhou], Korea and Vietnam)...
April 5, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29635210/evaluation-of-the-architect-hiv-ag-ab-combo-assay-in-a-low-prevalence-setting-the-role-of-samples-with-a-low-s-co-ratio
#20
Roberto Alonso, Felipe Pérez-García, Paloma Gijón, Ana Collazos, Emilio Bouza
BACKGROUND: The Architect HIV Ag/Ab Combo Assay, a fourth-generation ELISA, has proven to be highly reliable for the diagnosis of HIV infection. However, its high sensitivity may lead to false-positive results. OBJECTIVES: To evaluate the diagnostic performance of Architect in a low-prevalence population and to assess the role of the sample-to-cutoff ratio (S/CO) in reducing the frequency of false-positive results. STUDY DESIGN: We conducted a retrospective study of samples analyzed by Architect between January 2015 and June 2017...
April 4, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
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