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Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology

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https://www.readbyqxmd.com/read/28088665/influenza-a-h1n1-pdm-2009-and-influenza-b-virus-co-infection-in-hospitalized-and-non-hospitalized-patients-during-the-2015-2016-epidemic-season-in-israel
#1
Rakefet Pando, Yaron Drori, Nehemya Friedman, Aharona Glatman-Freedman, Hanna Sefty, Tamar Shohat, Ella Mendelson, Musa Hindiyeh, Michal Mandelboim
BACKGROUND: Influenza A and B viruses co-infections are rare events and mainly occurred in immunocompromised patients. OBJECTIVES: In this study we report an unusually high occurrence of influenza A (H1N1)pdm 2009 and influenza B virus co-infections during the epidemic year 2015-2016. STUDY DESIGN: Nasopharyngeal swabs were collected from 1919 patients visiting 26 outpatient clinics distributed throughout Israel and presenting with influenza-like illness...
January 6, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28107671/recent-sequence-variation-in-probe-binding-site-affected-detection-of-respiratory-syncytial-virus-group-b-by-real-time-rt-pcr
#2
Everlyn Kamau, Charles N Agoti, Clement S Lewa, John Oketch, Betty E Owor, Grieven P Otieno, Anne Bett, Patricia A Cane, D James Nokes
BACKGROUND: Direct immuno-fluorescence test (IFAT) and multiplex real-time RT-PCR have been central to RSV diagnosis in Kilifi, Kenya. Recently, these two methods showed discrepancies with an increasing number of PCR undetectable RSV-B viruses. OBJECTIVES: Establish if mismatches in the primer and probe binding sites could have reduced real-time RT-PCR sensitivity. STUDY DESIGN: Nucleoprotein (N) and glycoprotein (G) genes were sequenced for real-time RT-PCR positive and negative samples...
January 5, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28081453/intertypic-recombination-of-human-parechovirus-4-isolated-from-infants-with-sepsis-like-disease
#3
Pekka Kolehmainen, Anu Siponen, Teemu Smura, Hannimari Kallio-Kokko, Olli Vapalahti, Anne Jääskeläinen, Sisko Tauriainen
BACKGROUND: Human parechoviruses (HPeVs) (family Picornaviridae), are common pathogens in young children. Despite their high prevalence, research on their genetic identity, diversity and evolution have remained scarce. OBJECTIVES: Complete coding regions of three previously reported HPeV-4 isolates from Finnish children with sepsis-like disease were sequenced in order to elucidate the phylogenetic relationships and potential recombination events during the evolution of these isolates...
January 3, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28088027/an-alternative-strategy-to-western-blot-as-a-confirmatory-diagnostic-test-for-hiv-infection
#4
Xia Feng, Jibao Wang, Zhiyun Gao, Yu Tian, Ling Zhang, Huichao Chen, Tong Zhang, Lin Xiao, Jun Yao, Wenge Xing, Maofeng Qiu, Yan Jiang
BACKGROUND: In China, western blot (WB) is the recommended procedure for the diagnosis of HIV infection. However, this technique is time consuming and labor intensive, and its complexity restricts wide application in resource-limited regions. OBJECTIVE: The aim of this study was to evaluate the efficacy of a dry blood spots (DBS)-urine paired enzyme-linked immunosorbent assay (ELISA) test, instead of WB, for HIV antibody detection. STUDY DESIGN: Plasma, DBS, and urine samples were collected from 1213 subjects from different populations...
December 21, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28033514/infections-due-to-alphaherpesviruses-in-early-post-transplant-period-after-allogeneic-haematopoietic-stem-cell-transplantation-results-of-a-5-year-survey
#5
Maciej Przybylski, Anna Majewska, Tomasz Dzieciatkowski, Patrycja Rusicka, Grzegorz W Basak, Barbara Nasilowska-Adamska, Jaroslaw Bilinski, Wieslaw W Jedrzejczak, Marta Wroblewska, Kazimierz Halaburda, Grazyna Mlynarczyk, Agnieszka Tomaszewska
BACKGROUND: Infections caused by human α-herpesviruses usually have a benign course with recurrencies. However, they may become dangerous in immunocompromised hosts. In this case, molecular methods constitute a reliable diagnostic tool enabling rapid assessment of the efficacy of antiviral treatment strategies. OBJECTIVES: We estimated the frequency of alphaherpesviral DNAemia and the viral load during early post-transplantation period after alloHSCT; we also analyzed association of the DNAemia and chosen parameters of the patients...
December 21, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28033513/norovirus-in-feces-and-nasopharyngeal-swab-of-children-with-and-without-acute-gastroenteritis-symptoms-first-report-of-gi-5-in-brazil-and-gi-3-in-nasopharyngeal-swab
#6
Nathânia Dábilla, Tâmera Nunes Vieira Almeida, Anniely Carvalho Rebouças Oliveira, André Kipnis, Thairiny Neres Silva, Fabíola Souza Fiaccadori, Teresinha Teixeira de Sousa, Divina das Dôres de Paula Cardoso, Menira Souza
BACKGROUND: Noroviruses (NoVs) are an important cause of acute gastroenteritis (AGE), worldwide. OBJECTIVES: To evaluate the frequency, viral load and molecular profile of NoV in fecal and nasopharyngeal swab samples from hospitalized children, and to determine children's secretor status. STUDY DESIGN: From May 2014 to May 2015, 219 children were included in the study, 96 with gastroenteric symptoms and 123 without gastroenteric symptoms. All fecal and nasopharyngeal swab samples were screened by TaqMan RT-qPCR duplex (GI/GII NoV) and quality samples were characterized by genomic sequencing...
December 21, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28095350/progressive-multifocal-leukoencephalopathy-in-a-patient-with-systemic-mastocytosis-treated-with-cladribine
#7
Karl B Alstadhaug, Randi Fykse Halstensen, Francis Odeh
BACKGROUND: Progressive multifocal leukoencephalopathy (PML) is a rare opportunistic brain infection caused by the human polyomavirus JC (JCPyV). A particular problem with the drug cladribine seems to be prolonged suppression of the CD4+ T-cells, a well-known risk factor for PML. CASE DESCRIPTION: A 67-year-old male presented with a 3-weeks history of unsteady gait, dysarthria and a dysfunctional right arm. Seven years earlier, he had been diagnosed with urticaria pigmentosa, and 2 years later aggressive systemic mastocytosis...
December 14, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27992790/association-between-high-risk-human-papillomavirus-infection-and-co-infection-with-candida-spp-and-trichomonas-vaginalis-in-women-with-cervical-premalignant-and-malignant-lesions
#8
Ishita Ghosh, Richard Muwonge, Srabani Mittal, Dipanwita Banerjee, Pratip Kundu, Ranajit Mandal, Jaydip Biswas, Partha Basu
BACKGROUND: Human papillomavirus (HPV) is the necessary cause of cervical cancer. Cervico-vaginal infection with pathogens like Chlamydia is a likely cofactor. The interactions between HPV, Trichomonas vaginalis (TV) and Candida spp. are less understood, though inflammation induced by these pathogens has been demonstrated to facilitate oncogenesis. OBJECTIVE: Our study aimed to evaluate the association between Candida spp. and TV co-infection with HPV in cervical oncogenesis...
December 12, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27992789/analysis-of-aichi-virus-and-saffold-virus-association-with-pediatric-acute-gastroenteritis
#9
Li-Li Li, Na Liu, Jei-Mei Yu, Yuan-Yun Ao, Shan Li, O Colin Stine, Zhao-Jun Duan
BACKGROUND: Aichi virus (AiV) and Saffold virus (SAFV) have been reported in children with acute gastroenteritis and respiratory disease worldwide; however, their causative role in acute gastroenteritis remains ambiguous. OBJECTIVES: To assess the clinical association of AiV and SAFV with acute gastroenteritis in the pediatric population. STUDY DESIGN: A case-control study involving 461 paired stool samples from pediatric cases with diarrhea and healthy controls was conducted in China...
December 12, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27987421/successful-oral-treatment-of-ganciclovir-resistant-cytomegalovirus-with-maribavir-in-the-context-of-primary-immunodeficiency-first-case-report-and-review
#10
Philip D Bright, Mark Gompels, Matthew Donati, Sarah Johnston
No abstract text is available yet for this article.
December 12, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27988420/aptima-hpv-assay-versus-hybrid-capture-%C3%A2-2-hpv-test-for-primary-cervical-cancer-screening-in-the-hpv-focal-trial
#11
Darrel A Cook, Laurie W Smith, Jennifer Law, Wendy Mei, Dirk J van Niekerk, Kathy Ceballos, Lovedeep Gondara, Eduardo L Franco, Andrew J Coldman, Gina S Ogilvie, Dan Jang, Max Chernesky, Mel Krajden
BACKGROUND: Cervical cancer screening programs are switching from Pap screening to high-risk HPV testing. OBJECTIVES: To compare the Aptima HPV Assay (AHPV) with the Hybrid Capture(®) 2 High-Risk HPV DNA Test(®) (HC2) for primary cervical screening. STUDY DESIGN: HPV FOCAL is a randomized trial comparing HC2 to liquid-based cytology (LBC) for screening women aged 25-65. AHPV and HC2 were compared at the baseline screen (n=3473). Genotyping was by the Aptima HPV 16 18/45 Genotype Assay...
December 11, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27987422/diagnostic-performance-of-serological-assays-for-anti-hbs-testing-results-from-a-quality-assessment-program
#12
Stijn Raven, Jeannine Hautvast, Jim van Steenbergen, Reinier Akkermans, Cas Weykamp, Francis Smits, Christian Hoebe, Ann Vossen
BACKGROUND: Post-vaccination testing after hepatitis B vaccination is indispensable to evaluate long-term immunological protection. Using a threshold level of antibodies against hepatitis B surface antigen (anti-HBs) to define serological protection, implies reproducible and valid measurements of different diagnostic assays. OBJECTIVES: In this study we assess the performance of currently used anti-HBs assays. STUDY DESIGN: In 2013, 45 laboratories participated in an external quality assessment program using pooled anti-HBs serum samples around the cutoff values 10IU/l and 100IU/l...
December 7, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28011413/a-systematic-review-and-meta-analysis-of-seroprevalence-of-varicella-zoster-virus-a-nationwide-population-based-study
#13
REVIEW
Omolbanin Amjadi, Alireza Rafiei, Mohammadreza Haghshenas, Reza Alizadeh Navaei, Reza Valadan, Zahra Hosseini-Khah, Akbar Hedayatizadeh Omran, Mohsen Arabi, Reza Jafari Shakib, Tahoora Mousavi, G Hossein Ashrafi
Varicella zoster virus (VZV) causes chicken pox as a primary infection following which it becomes latent in neurons. It may then reactivate to cause shingles (herpes zoster). Severity of lesions and VZV pathogenicity are depended on the host's immune response and variant in VZV Dr Athina Myrto ChioniIdentification of VZV seroprevalance rate in general population may lead to develop new health strategic managements such as vaccination. Therefore, we aimed to provide a systematic review of the seroprevalence of VZV infection among Iranian population and estimate age- and gender- specific prevalence of VZV...
December 6, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27992788/efficacy-safety-and-pharmacokinetics-of-atazanavir-200mg-twice-daily-plus-raltegravir-400mg-twice-daily-dual-regimen-in-the-clinical-setting
#14
Letizia Marinaro, Andrea Calcagno, Diego Ripamonti, Giovanni Cenderello, Veronica Pirriatore, Laura Trentini, Bernardino Salassa, Caterina Bramato, Giancarlo Orofino, Antonio D'Avolio, Marco Rizzi, Giovanni Di Perri, Stefano Rusconi, Stefano Bonora
BACKGROUND: Unboosted atazanavir with raltegravir has been investigated at 300mg twice daily showing frequent hyperbilirubinemia and selection of resistance-associated mutations. OBJECTIVES: Atazanavir 200mg twice daily could increase tolerability and plasma exposure. STUDY DESIGN: Patients on atazanavir/raltegravir (200/400 twice daily), with self-reported adherence >95% and no concomitant interacting drugs were retrospectively evaluated...
December 1, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27984766/diagnostic-usefulness-of-dynamic-changes-of-cmv-specific-t-cell-responses-in-predicting-cmv-infections-in-hct-recipients
#15
Jiwon Jung, Hyun-Jung Lee, Sun-Mi Kim, Young-Ah Kang, Young-Shin Lee, Yong Pil Chong, Heungsup Sung, Sang-Oh Lee, Sang-Ho Choi, Yang Soo Kim, Jun Hee Woo, Jung-Hee Lee, Je-Hwan Lee, Kyoo-Hyung Lee, Sung-Han Kim
BACKGROUND: CMV-specific cell mediated immune responses before and after hematopoietic stem cell transplantation (HCT) can categorize patients as at high or low risk of CMV development. OBJECTIVES: We evaluated the usefulness of the CMV-specific T-cell ELISPOT assay for predicting the development of CMV infections after HCT in recipients with donor-positive and recipient-positive CMV serology (D+/R+ ). STUDY DESIGN: CMV pp65 and IE1-specific ELISPOT assays were performed before HCT (D0), and at 30 (D30) and 90 (D90) days after HCT...
November 30, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27984765/hpv-prevalence-in-women-attending-cervical-screening-in-rural-malawi-using-the-cartridge-based-xpert-%C3%A2-hpv-assay
#16
Heather A Cubie, David Morton, Edson Kawonga, Mike Mautanga, Ipyana Mwenitete, Ngari Teakle, Bagrey Ngwira, Hilary Walker, Graeme Walker, Savel Kafwafwa, Beatrice Kabota, Reynier Ter Haar, Christine Campbell
BACKGROUND AND OBJECTIVES: Early experience with Cepheid Xpert(®) HPV assay (Xpert(®) HPV) suggests that its quick turnaround time and ease of application might make it a relevant contender for routine use in low and middle income countries (LMICs). In the context of a cervical screening service in rural Malawi, we aimed to assess practicalities of local laboratory testing with Xpert(®) HPV and provide preliminary high-risk HPV (HR-HPV) prevalence data. STUDY DESIGN: Liquid-based cytology (LBC) specimens were collected from women attending cervical screening clinics in Nkhoma, Malawi...
November 30, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27846440/evaluation-of-human-papillomavirus-detection-by-abbott-m2000-system-on-samples-collected-by-fta-elute%C3%A2-card-in-a-chinese-hiv-1-positive-population
#17
Yu Qin, Hongyun Zhang, Natalia Marlowe, Mandong Fei, Judy Yu, Xiaoqin Lei, Lulu Yu, Jia Zhang, Di Cao, Li Ma, Wen Chen
BACKGROUND: HIV+/AIDS women have an increased risk of developing into CIN and cervical cancer compared to the general population. Limited medical resource and the lack of AIDS relevant knowledge impair the coverage and efficiency of cervical cancer screening. OBJECTIVES: To compare the clinical performance of self-collected dry storage medium (FTA Elute card) and physician-collected PreservCyt medium in detection of high risk human papillomavirus (HR HPV) among HIV-1 positive population...
November 9, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27835758/association-of-cytologic-grade-of-anal-pap-smears-with-viral-loads-of-human-papillomavirus-types-16-18-and-52-detected-in-the-same-specimens-from-men-who-have-sex-with-men
#18
Utaiwan Utaipat, Sumalee Siriaunkgul, Taweewat Supindham, Pongpun Saokhieo, Butsayarat Chaidaeng, Antika Wongthanee, Jongkolnee Settakorn, Kornkanok Sukpan, Darin Ruanpeng, Natthapol Kosashunhanan, Nuntisa Chotirosniramit, Patcharaphan Sugandhavesa, Toshiyuki Miura, Suwat Chariyalertsak
BACKGROUND: Human papilloma virus (HPV) load has been linked to cellular abnormalities of the uterine cervix, and proposed as predictors of HPV persistence and progression of dysplasia to cervical cancer. However, the association of HPV viral load and anal dysplasia and cancer has not been as thoroughly investigated. OBJECTIVES: To examine the association of the viral loads of high-risk HPV types 16, 18, and 52, with the cytologic severity grading in anal-swab specimens of MSM with and without HIV-1 co-infection...
November 6, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27835760/performance-evaluation-of-a-rapid-molecular-diagnostic-multicode-based-sample-to-answer-assay-for-the-simultaneous-detection-of-influenza-a-b-and-respiratory-syncytial-viruses
#19
Jolanda J C Voermans, S Seven-Deniz, P L A Fraaij, Annemiek A van der Eijk, M P G Koopmans, Suzan D Pas
BACKGROUND: Clinical signs and symptoms of different airway pathogens are generally indistinguishable, making laboratory tests essential for clinical decisions regarding isolation and antiviral therapy. Immunochromatographic tests (ICT) and direct immunofluorescence assays (DFA) have lower sensitivities and specificities than molecular assays, but have the advantage of quick turnaround times and ease-of-use. OBJECTIVE: To evaluate the performance of a rapid molecular assay, ARIES FluA/B & RSV, using laboratory developed RT-PCR assays (LDA), ICT (BinaxNOW) and DFA...
November 3, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27835757/vaccine-strain-herpes-zoster-found-in-the-trigeminal-nerve-area-in-a-healthy-child-a-case-report
#20
Sayaka Iwasaki, Kouji Motokura, Yoshitaka Honda, Masamitsu Mikami, Daisuke Hata, Atsuko Hata
A previously healthy 2-year-old girl, vaccinated for varicella at 17 months, was admitted because of left-sided facial herpes zoster caused by vaccine-strain varicella-zoster virus (VZV). She recovered fully with no complication after intravenous treatment using acyclovir. Earlier reports have described that herpes zoster (HZ) rashes caused by vaccine-strain VZV tend to occur on the dermis corresponding to the skin area where the varicella vaccine was received. However, rashes appeared on this girl only in the trigeminal nerve area, which is unrelated to the vaccinated site...
November 3, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
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