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Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology

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https://www.readbyqxmd.com/read/28918127/clinical-laboratory-and-virological-data-from-suspected-zikv-patients-in-an-endemic-arbovirus-area
#1
Tatiana Elias Colombo, Cássia Fernanda Estofolete, Andréia Francesli Negri Reis, Natal Santos da Silva, Morgana Lima Aguiar, Eliana Márcia Sotello Cabrera, Izalco Nuremberg Penha Dos Santos, Fabiana Rodrigues Costa, Lilian Elisa Arão Antônio Cruz, Patrícia Lopes Rombola, Ana Carolina Bernardes Terzian, Maurício Lacerda Nogueira
BACKGROUND: The emergence of Zika virus (ZIKV) presents new challenges to both clinicians and public health authorities. Overlapping clinical features between the diseases caused by ZIKV, dengue (DENV) and chikungunya (CHIKV) and the lack of validated serological assays for ZIKV make accurate diagnosis difficult. Brazilian authorities largely rely on clinical and epidemiological data for the epidemiological and clinical classifications of most ZIKV cases. OBJECTIVE: To report the laboratory and clinical profiles of patients diagnosed with Zika fever based only on clinical and epidemiological data...
September 9, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28915451/rotavirus-genotype-shifts-among-swedish-children-and-adults-application-of-a-real-time-pcr-genotyping
#2
Maria Andersson, Magnus Lindh
BACKGROUND: It is well known that human rotavirus group A is the most important cause of severe diarrhoea in infants and young children. Less is known about rotavirus infections in other age groups, and about how rotavirus genotypes change over time in different age groups. OBJECTIVES: Develop a real-time PCR to easily genotype rotavirus strains in order to monitor the pattern of circulating genotypes. STUDY DESIGN: In this study, rotavirus strains in clinical samples from children and adults in Western Sweden during 2010-2014 were retrospectively genotyped by using specific amplification of VP 4 and VP 7 genes with a new developed real-rime PCR...
September 9, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28898704/interpretation-of-indeterminate-hiv-1-pcr-results-are-influenced-by-changing-vertical-transmission-prevention-regimens
#3
Jean Maritz, Jayshree Narvin Maharaj, Mark Frederic Cotton, Wolfgang Preiser
BACKGROUND: Suppression of HIV by antiretroviral drugs may be one of the reasons that indeterminate HIV-1 PCR results are obtained from testing HIV-exposed infants. This complicates the early identification of infected infants, potentially delaying initiating treatment early. There is uncertainty as to how different vertical HIV transmission prevention regimens (VTP) affect the rate and predictive value of indeterminate PCR results. OBJECTIVES: To investigate rates of indeterminate PCR results, outcomes of subsequent samples and the predictive value of an indeterminate PCR for a later positive result in the setting of intensifying VTP in the Western Cape province of South Africa...
September 9, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28915452/bk-virus-replication-in-renal-transplant-recipients-analysis-of-potential-risk-factors-may-contribute-in-reactivation
#4
Mohammad Shenagari, Ali Monfared, Hadise Eghtedari, Aydin Pourkazemi, Tolou Hasandokht, Masoud Khosravi, Babak Asharfkhani
BACKGROUND: Considering the increasing problem of BK virus infection during post renal transplant surveillance, it is necessary to distinguish the main risk factors leading to reactivation of latent BK virus. Up to now, some probable risk factors have been investigated in some studies, but the results have been confusing and contradictory. OBJECTIVES: The goal of the present study was to determine the frequency and potential risk factors that may play a role in BK polyomavirus reactivation and nephropathy...
September 8, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28892764/a-european-multicientre-study-on-the-comparison-of-hbv-viral-loads-between-veris-hbv-assay-and-roche-cobas-%C3%A2-taqman-%C3%A2-hbv-test-abbott-realtime-hbv-assay-siemens-versant-hbv-assay-and-qiagen-artus-hbv-rg-kit
#5
Patrick Braun, Rafael Delgado, Monica Drago, Diana Fanti, Hervé Fleury, Jacques Izopet, Alessandra Lombardi, MaAngeles Marcos, Karine Sauné, Siobhan O'Shea, Alfredo Pérez-Rivilla, John Ramble, Pascale Trimoulet, Jordi Vila, Duncan Whittaker, Alain Artus, Daniel Rhodes
BACKGROUND: Hepatitis B viral load testing is essential to treatment and monitoring decisions in patients with chronic Hepatitis B. Beckman Coulter has developed the VERIS HBV Assay (Veris) for use on the fully automated DxN VERIS Molecular Diagnostics System.(1) OBJECTIVES: To evaluate the clinical performance of the Veris HBV Assay at multiple EU laboratories STUDY DESIGN: Method comparison was performed with a total of 344 plasma specimens from HBV infected patients tested with Veris and COBAS(®) TaqMan(®) HBV Test (Cobas), 207 specimens tested with Veris and RealTime HBV Assay (RealTime), 86 specimens tested with Veris and VERSANT(®) HBV Assay (Versant), and 74 specimens tested with Veris and artus(®) HBV RG PCR kit (artus)...
September 8, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28918126/high-cmv-igg-antibody-levels-are-associated-to-a-lower-cd4-response-to-antiretroviral-therapy-in-hiv-infected-women
#6
Marina Giuliano, Maria Franca Pirillo, Giuseppe Liotta, Mauro Andreotti, Haswell Jere, Jean-Baptiste Sagno, Fausto Ciccacci, Roberta Amici, Maria Cristina Marazzi, Stefano Vella, Leonardo Palombi, Sandro Mancinelli
BACKGROUND: Virtually all HIV-infected women in sub-Saharan Africa have evidence of Cytomegalovirus (CMV) infection and levels of specific anti-CMV IgG have been suggested to represent more intense reactivation of subclinical infection. Studies have also shown direct influence of CMV on lymphocytes. OBJECTIVE: The aim of this study was to determine if levels of anti-CMV specific antibodies could impact on the immunological response to antiretroviral treatment (ART) in HIV-infected pregnant women...
September 5, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28892765/the-performance-of-luminex-aries-%C3%A2-flu-a-b-rsv-and-cepheid-xpert-%C3%A2-flu-rsv-xc-for-the-detection-of-influenza-a-influenza-b-and-respiratory-syncytial-virus-in-prospective-patient-samples
#7
Phillip McMullen, Sue Boonlayangoor, Angella Charnot-Katsikas, Kathleen G Beavis, Vera Tesic
BACKGROUND: The demand for rapid, accurate viral testing has increased the number of assays available for the detection of viral pathogens. One of the newest FDA cleared platforms is the Luminex ARIES(®) Flu A/B & RSV, which is a fully automated, real-time PCR-based assay used for detection of influenza A, influenza B, and respiratory syncytial virus (RSV). OBJECTIVES: We sought to compare the performance of Luminex ARIES(®) Flu A/B & RSV assay to the Cepheid Xpert(®) Flu/RSV XC assay for rapid Flu and RSV testing...
September 5, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28903080/transplacental-transfer-of-maternal-respiratory-syncytial-virus-rsv-antibody-and-protection-against-rsv-disease-in-infants-in-rural-nepal
#8
Helen Y Chu, James Tielsch, Joanne Katz, Amalia S Magaret, Subarna Khatry, Stephen C LeClerq, Laxman Shrestha, Jane Kuypers, Mark C Steinhoff, Janet A Englund
BACKGROUND: Respiratory syncytial virus (RSV) is the most important viral cause of pneumonia in children. RSV-specific antibody (ab) protects infants from disease, and may be increased by a potential strategy of maternal RSV vaccination. OBJECTIVES: To describe the effect of RSV antibody on RSV infection risk in infants in a resource-limited setting. STUDY DESIGN: In a prospective study in Nepal, women were enrolled during pregnancy and maternal and infant cord blood were collected at birth...
September 2, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28917132/a-novel-real-time-rt-pcr-assay-for-influenza-c-tested-in-peruvian-children
#9
Leigh M Howard, Monika Johnson, Ana I Gil, Andrew Pekosz, Marie R Griffin, Kathryn M Edwards, Claudio F Lanata, Carlos G Grijalva, John V Williams
BACKGROUND: Influenza C virus (ICV) is associated with acute respiratory illness. Yet ICV remains under recognized, with most previous studies using only culture to identify cases. OBJECTIVES: To develop a sensitive and specific real-time RT-PCR assay for ICV that allows for rapid and accurate detection in a clinical or research setting. STUDY DESIGN: Multiple ICV sequences obtained from GenBank were analyzed, including 141 hemagglutinin-esterase (HE), 106 matrix (M), and 97 nucleoprotein (NP) sequences...
September 1, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28889082/comparison-of-three-multiplex-gastrointestinal-platforms-for-the-detection-of-gastroenteritis-viruses
#10
Preeti Chhabra, Nicole Gregoricus, Geoffrey A Weinberg, Natasha Halasa, James Chappell, Ferdaus Hassan, Rangaraj Selvarangan, Slavica Mijatovic-Rustempasic, M Leanne Ward, Michael Bowen, Daniel C Payne, Jan Vinjé
BACKGROUND: Viruses are major etiological agents of childhood gastroenteritis. In recent years, several molecular platforms for the detection of viral enteric pathogens have become available. OBJECTIVE/STUDY DESIGN: We evaluated the performance of three multiplex platforms including Biofire's Gastrointestinal Panel (FilmArray), Luminex xTAG(®) Gastrointestinal Pathogen Panel (GPP), and the TaqMan Array Card (TAC) for the detection of five gastroenteritis viruses using a coded panel of 300 archived stool samples...
September 1, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28886462/genetic-polymorphism-of-thymidine-kinase-tk-and-dna-polymerase-pol-of-clinical-varicella-zoster-virus-vzv-isolates-collected-over-three-decades
#11
Anja Hoffmann, Kristin Döring, Natalja Tatjana Seeger, Martina Bühler, Michael Schacke, Andi Krumbholz, Andreas Sauerbrei
BACKGROUND: Genotypic resistance testing of varicella-zoster virus (VZV) strains to antivirals is of high relevance in immunocompromised patients with VZV reactivations unresponsive to therapy. However, the knowledge on mutations associated with natural gene polymorphism or resistance is limited. OBJECTIVES: To examine the genotype of the thymidine kinase (TK) and DNA polymerase (pol) of unselected clinical VZV isolates collected between 1984 and 2014 and to verify the phenotype related to novel amino acid (aa) substitutions...
September 1, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28889083/live-versus-attenuated-influenza-vaccine-uptake-and-post-vaccination-influenza-like-illness-outcomes-in-hiv-infected-us-air-force-members
#12
Julie L Menegay, Xiaohe Xu, T S Sunil, Jason F Okulicz
BACKGROUND: Although clinical data is limited, guidelines recommend avoiding live attenuated intranasal influenza vaccine (LAIV) in HIV-infected persons. OBJECTIVES: To evaluate non-guideline LAIV use and resulting adverse effects in an HIV-infected population. STUDY DESIGN: A retrospective analysis of influenza vaccination in US Air Force (USAF) members with HIV infection immunized between 2005 and 2015 was performed. Influenza vaccination history after HIV diagnosis was evaluated, including receipt of LAIV or inactivated influenza vaccine (IIV)...
August 25, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28869890/development-of-anti-hepatitis-b-surface-hbs-antibodies-after-hbs-antigen-loss-in-hiv-hepatitis-b-virus-co-infected-patients
#13
Anders Boyd, Laetitia Canini, Joël Gozlan, Caroline Lascoux-Combe, Patrick Miailhes, Laurent Fonquernie, Pierre-Marie Girard, Karine Lacombe
BACKGROUND: Hepatitis B surface antigen (HBsAg)-seroconversion, or loss of HBsAg and acquisition of anti-hepatitis B surface (HBs) antibodies, defines functional cure of chronic hepatitis B virus (HBV) infection. After HBsAg-loss, little is known regarding the development of anti-HBs antibodies and even less so in individuals co-infected with HIV. OBJECTIVES: To determine anti-HBs antibody kinetics after HBsAg-loss and explore determinants of HBsAg-seroconversion in HIV-HBV co-infected patients...
August 25, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28866135/congenital-zika-syndrome-with-prolonged-detection-of-zika-virus-rna
#14
LETTER
Wilmer E Villamil-Gómez, Ezequiel Guijarro, Jaime Castellanos, Alfonso J Rodríguez-Morales
No abstract text is available yet for this article.
August 25, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28843383/il-10-592-polymorphism-is-associated-with-il-10-expression-and-severity-of-enterovirus-71-infection-in-chinese-children
#15
Na Zhao, Hui-Lan Chen, Zhen-Zhen Chen, Jing Li, Zong-Bo Chen
BACKGROUND: Enterovirus 71 (EV71) infection results in some severe complications with high mortality and disability in Hand, Foot and Mouth Disease (HFMD) in children. Recent studies have shown that cytokine genetic predispositions have associations with both the development of EV71 infection and severity of HFMD. OBJECTIVE: This study was designed to investigate whether the IL-10-592 polymorphism is associated with IL-10 levels and disease severity in Chinese children with EV71 infection...
August 19, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28830013/corrigendum-to-implementation-of-hpv-testing-in-latin-america-j-clin-virol-76-s1-2016-s69-s73
#16
Jose Jeronimo, Francesca Holme, Rose Slavkovsky, Claudia Camel
No abstract text is available yet for this article.
August 19, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28843384/staging-of-recent-hiv-1-infection-using-geenius-rapid-confirmatory-assay-compared-to-inno-lia-new-lav-and-blot-2-2-assays
#17
E Tuaillon, A Sanosyan, A Pisoni, J Liscouët, A Makinson, P Van de Perre
BACKGROUND: Besides confirmation of HIV seropositivity, Western Blot (WB) assays play an important role for identification of recent infection based on incomplete antibody reactivity and lack of p31 band. OBJECTIVES: We evaluated the capacities of the Geenius™ HIV1/2 Confirmatory Assay (Bio-Rad), a new generation rapid confirmatory assay based on immune-chromatography and automated reading, for staging of HIV-1 infection. STUDY DESIGN: Sixteen samples collected during early HIV-1 infections (Fiebig stage III-VI) were tested using the Geenius assay, and compared to HIV Blot 2...
August 18, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28843110/cytomegalovirus-reactivation-in-lymphoma-and-myeloma-patients-undergoing-autologous-peripheral-blood-stem-cell-transplantation
#18
Radwan Massoud, Rita Assi, Elie Fares, Basel Haffar, Maya Charafeddine, Nabila Kreidieh, Rami Mahfouz, Souha S Kanj, Aline El Zakhem, Mohamed Kharfan-Dabaja, Ali Bazarbachi, Jean El Cheikh
BACKGROUND: Cytomegalovirus reactivation is often diagnosed in allogeneic hematopoietic cell transplant recipients and therefore could lead to CMV-related disease, involving many organs in these immunocompromised patients. In contrast, few studies investigated CMV reactivation and end-organ disease in patients undergoing Autologous Peripheral Blood Stem Cell Transplant (ASCT) since they are considered at low risk for both reactivation and disease. OBJECTIVES: The primary outcome of the analysis was to understand the difference in incidence of CMV reactivation between MM and Lymphoma patients...
August 18, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28818690/early-estimates-of-2016-17-seasonal-influenza-vaccine-effectiveness-in-primary-care-in-france
#19
Cécile Souty, Ana-Maria Vilcu, Lisandru Capai, Sylvie van der Werf, Martine Valette, Thierry Blanchon, Bruno Lina, Sylvie Behillil, Thomas Hanslik, Alessandra Falchi
BACKGROUND: The ongoing 2016/17 influenza epidemic in France is characterized by the circulation of A(H3N2) viruses, known to cause more severe illness among at risk populations. OBJECTIVES: The purpose of our study was to provide early influenza vaccine effectiveness (IVE) estimates for the ongoing influenza epidemic in France and compare these estimates over the six post-pandemic IVE. STUDY DESIGN: We used clinical and virological data collected in primary care by the French Sentinelles network...
August 9, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28822317/on-the-utility-of-the-benzonase-treatment-for-correct-laboratory-diagnosis-of-parvovirus-b19-infection
#20
LETTER
Ulrike Reber, Olga Moser, Dagmar Dilloo, Anna M Eis-Hübinger
No abstract text is available yet for this article.
August 8, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
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