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Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology

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https://www.readbyqxmd.com/read/30031351/diagnostic-accuracy-of-digital-rna-quantification-versus-real-time-pcr-for-the-detection-of-respiratory-syncytial-virus-in-nasopharyngeal-aspirates-from-children-with-acute-respiratory-infection
#1
Maiara L Bouzas, Juliana R Oliveira, Artur Queiroz, Kiyoshi F Fukutani, Aldina Barral, Annabel Rector, Elke Wollants, Els Keyaerts, Winke Van der Gucht, Marc Van Ranst, Kurt Beuselinck, Camila I de Oliveira, Johan Van Weyenbergh, Cristiana M Nascimento-Carvalho
BACKGROUND: Virus-specific molecular assays such as real-time polymerase chain reaction (RT-PCR) are regularly used as the gold standard to diagnose viral respiratory tract infections, but simultaneous detection of multiple different pathogens is often challenging. A multiplex digital method of RNA quantification, nCounter (NanoString Technologies), can overcome this disadvantage and identify, in a single reaction, the presence of different respiratory viruses. OBJECTIVES: To evaluate the accuracy of nCounter to identify and quantify RSV-A and RSV-B in nasopharyngeal aspirates (NPA) of children (6-23-months-old) with acute respiratory infection...
July 18, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/30031350/re-lack-of-zika-virus-antibody-response-in-confirmed-patients-in-non-endemic-countries
#2
LETTER
Ralph Huits, Dorien Van Den Bossche, Emmanuel Bottieau, Marjan Van Esbroeck
No abstract text is available yet for this article.
July 17, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/30007138/evaluation-of-the-virtues-and-pitfalls-in-an-hiv-screening-algorithm-based-on-two-fourth-generation-assays-a-step-towards-an-improved-national-algorithm
#3
Boaz Avidor, Daniel Chemtob, Dan Turner, Irene Zeldis, Shirley Girshengorn, Natalia Matus, Svetlana Achsanov, Simona Gielman, Inbal Schweizer, Lilya Baskin, Licita Schreiber, Zipi Kra-Oz
BACKGROUND: Fourth-generation immunoassays used for HIV screening, simultaneously detect anti-HIV antibodies and HIV-1 P24 antigen, but are prone to false-positive results. Usually, they are followed by highly specific third-generation assay, able to differentiate between HIV-1/2 infections. In Israel, screening algorithm is based on consecutive testing by two fourth-generation assays and confirmation by a third-generation test. OBJECTIVES: To evaluate the performance of this algorithm...
July 9, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/30015285/combination-random-isothermal-amplification-and-nanopore-sequencing-for-rapid-identification-of-the-causative-agent-of-an-outbreak
#4
Sören Hansen, Oumar Faye, Sabri S Sanabani, Martin Faye, Susanne Böhlken-Fascher, Ousmane Faye, Amadou A Sall, Michaël Bekaert, Manfred Weidmann, Claus-Peter Czerny, Ahmed Abd El Wahed
BACKGROUND: Outbreaks of fever of unknown origin start with nonspecific symptoms and case definition is only slowly developed and adapted, therefore, identifying the causative agent is crucial to ensure suitable treatment and control measures. As an alternative method for Polymerase Chain Reaction in molecular diagnostics diagnostic, metagenomics can be applied to identify the pathogen responsible for the outbreak through sequencing all nucleic acids present in a sample extract. Sequencing data obtained can identify new or variants of known agents...
July 6, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/30007137/comparison-of-the-biofire-filmarray-respiratory-panel-seegene-anyplexii-rv16-and-argene-for-the-detection-of-respiratory-viruses
#5
Maurice Chan, Seok Hwee Koo, Boran Jiang, Pei Qi Lim, Thean Yen Tan
BACKGROUND: Respiratory infections are common reasons for hospital admission, and are associated with enormous economic burden due to significant morbidity and mortality. The wide spectrum of microbial agents underlying the pathology renders the diagnosis of respiratory infections challenging. Molecular diagnostics offer an advantage to the current serological and culture-based methods in terms of sensitivity, coverage, hands-on time, and time to results. OBJECTIVES: This study aimed to compare the clinical performance of three commercial kits for respiratory viral detection...
July 6, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/30007136/evaluation-of-a-rapid-point-of-care-hiv-screening-program-in-an-emergency-department-setting-in-detroit-michigan
#6
LETTER
Weiming Zhu, Kimberly Mumby, Doreen Dankerlui, Jake Manteuffel, Cal Ham, Ya-Lin A Huang, Philip J Peters, Yetunde F Fakile, Norman Markowitz, Karen W Hoover
No abstract text is available yet for this article.
June 28, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/30007135/unrecognized-prolonged-viral-replication-in-the-pathogenesis-of-human-rsv-infection
#7
Bindiya Bagga, L Harrison, P Roddam, J P DeVincenzo
BACKGROUND: Respiratory symptoms in RSV persist long after the virus is no longer detected by culture. Current concepts of RSV pathogenesis explain this by RSV inducing a long-lasting pathogenic immune cascade. We alternatively hypothesized that prolonged unrecognized RSV replication may be responsible and studied this possibility directly in a human wild-type RSV experimental infection model. OBJECTIVE: The objective of the current report was to define the duration of true human RSV replication by studying it directly in immunocompetent adults experimentally infected with a clinical strain of RSV utilizing this previously established safe and reproducible model...
June 27, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/30007139/evaluating-the-aptima-hiv-1-quant-dx-hcv-quant-dx-and-hbv-quant-assays-against-the-abbott-hiv-1-hcv-and-hbv-realtime-assays
#8
Shoshanna May, Ewelina Adamska, J W Tang
BACKGROUND: Viral load measurement is routine for the management of patients infected with HIV, HCV or HBV, using sensitive, quantitative, nucleic acid amplification tests (NAATs). However, platforms are not equivalent in terms of turnaround time, random access capability and operator hands-on time. OBJECTIVES AND STUDY DESIGN: We compared the performance of the Hologic Panther transcription-mediated amplification based Aptima assays for HIV, HBV and HCV viral load measurement with the corresponding Abbott m2000 RealTime assays...
June 25, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29957544/a-fully-automated-system-using-transcription-mediated-amplification-for-the-molecular-diagnosis-of-hepatitis-e-virus-in-human-blood-and-faeces
#9
Florence Abravanel, Sébastien Lhomme, Sabine Chapuy-Regaud, Jean-Michel Mansuy, Jérôme Boineau, Karine Sauné, Jacques Izopet
BACKGROUND AND OBJECTIVES: We evaluated the performance of the Procleix HEV RNA assay implemented on the Panther automated platform for detecting HEV RNA. STUDY DESIGN AND RESULTS: Analytical specificity was 100% and there was no cross contamination, as assessed by assaying 122 plasma samples from HEV RNA-negative blood donors. The limits of detection were determined by Probit analysis with the WHO HEV standard (HEV subtype 3a) and subtype 3f and 3c reference strains...
June 22, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29957545/dolutegravir-dtg-containing-regimens-after-receiving-raltegravir-ral-or-elvitegravir-evg-durability-and-virological-response-in-a-large-italian-hiv-drug-resistance-network-arca
#10
S Rusconi, F Adorni, P Tau, V Borghi, M Pecorari, R Maserati, D Francisci, L Monno, G Punzi, P Meraviglia, S Paolucci, A Di Biagio, B Bruzzone, A Mancon, V Micheli, M Zazzi
BACKGROUND: Dolutegravir (DTG) is a next-generation HIV integrase inhibitor (INI) with an increased genetic barrier to resistance with respect to raltegravir (RAL) or elvitegravir (EVG). Few data are available on the durability of DTG-containing regimens. OBJECTIVES: We aimed at investigating the duration of the DTG-containing regimen, the occurrence of an HIV-1 RNA blip, and factors associated with DTG virological response. STUDY DESIGN: From the Antiviral Response Cohort Analysis database, we selected 89 HIV-1-positive four-class-experienced subjects who started DTG after receiving RAL or EVG...
June 21, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29936407/t-track-cmv-and-quantiferon-cmv-assays-for-prediction-of-protection-from-cmv-reactivation-in-kidney-transplant-recipients
#11
Smaranda Gliga, Johannes Korth, Adalbert Krawczyk, Benjamin Wilde, Peter A Horn, Oliver Witzke, Monika Lindemann, Melanie Fiedler
BACKGROUND: Assays detecting CMV-specific cell-mediated immunity (CMI) may support the current management of CMV infection in solid-organ transplant (SOT) recipients, by allowing a better risk assessment and adjusting antiviral treatment. OBJECTIVES: The primary endpoint was the performance of two tests measuring CMV-specific interferon-gamma production, both approved for commercial use in clinical settings. Secondarily, we determined a cut-off for the cellular immune response, which protects against CMV reactivation/infection...
June 12, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29929067/inconsistent-detection-of-an-evolving-hiv-1-infection-by-a-popular-high-throughput-screening-assay
#12
LETTER
James B Veater, Judi Gardener, Benedict Rogers, Iain Stephenson, Julian W Tang
No abstract text is available yet for this article.
June 12, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29957546/development-of-a-standardized-real-time-pcr-for-torque-teno-viruses-ttv-viral-load-detection-and-quantification-a-new-tool-for-immune-monitoring
#13
Dorian Kulifaj, Bénédicte Durgueil-Lariviere, Faustine Meynier, Eliza Munteanu, Nicolas Pichon, Manon Dubé, Martine Joannes, Marie Essig, Sébastien Hantz, Côme Barranger, Sophie Alain
BACKGROUND: Torque teno viruses (TTV) are small DNA viruses whose replication is closely linked to immune status. A growing number of publications underlined the potential of TTV viral load as an indicator of immunosuppression. OBJECTIVES: To demonstrate the analytical performance of the first standardized RUO (Research Use Only) assay to detect and quantify human TTV DNA in whole blood and plasma. STUDY DESIGN: We established analytical performances for TTV load measurement in various populations...
June 11, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29940422/evaluation-of-in-vitro-screening-and-diagnostic-kits-for-hepatitis-c-virus-infection
#14
Haruka Momose, Sahoko Matsuoka, Asako Murayama, Norie Yamada, Kazu Okuma, Emi Ikebe, Yuji Hoshi, Masamichi Muramatsu, Takaji Wakita, Kuro Toyota, Takanobu Kato, Isao Hamaguchi
BACKGROUND: To detect infection by hepatitis C virus (HCV), a reliable kit with high sensitivity and specificity is indispensable. Detection kits for anti-HCV antibodies (anti-HCV) are used for screening, and quantification kits for HCV RNA and core antigen are used for definite diagnosis of HCV infection. OBJECTIVES: We evaluated the performance of these kits using International Standards and a regional reference panel with HCV negative and positive specimens. STUDY DESIGN: In vitro diagnostic kits (10 anti-HCV, two HCV RNA, and three HCV core antigen) were included...
June 11, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29940421/novel-score-predicts-risk-for-cytomegalovirus-infection-in-ulcerative-colitis
#15
Tobias M Nowacki, Dominik Bettenworth, Tobias Meister, Jan Heidemann, Frank Lenze, Hartmut H Schmidt, Hauke S Heinzow
BACKGROUND: Cytomegalovirus (CMV) infection is associated with relapse and exacerbation of ulcerative colitis (UC), especially in immunosuppressed patients. OBJECTIVES: The aim of this study was to identify risk factors for CMV colitis and to develop a predictive risk score to estimate the probability of CMV colitis in UC patients supporting clinical decision making. STUDY DESIGN: A cohort of 239 UC-patients was retrospectively analyzed. Univariate and multivariate regression analysis identified several independent risk factors for CMV colitis and a predictive risk score was established using ROC analysis...
June 9, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29886372/genome-wide-linear-b-cell-epitopes-of-enterovirus-71-in-a-hand-foot-and-mouth-disease-hfmd-population
#16
Huiying Zhang, Zhigang Song, Huiju Yu, Xiaoling Zhang, Shanshan Xu, Zhong Li, Jingzhi Li, Hongke Xu, Zhenghong Yuan, Hongwei Ma, Zhigang Yi, Yunwen Hu
BACKGROUND: Enteroviruses cause hand, foot and mouth disease (HFMD). The host B-cells recognize the viral proteins and provoke humoral responses. Deciphering the B-cell responses to the viral epitopes helps diagnosis and vaccine development. OBJECTIVES: The objective of the present study was to investigate for the first time the landscape of genome-wide linear B-cell epitopes of enterovirus 71 in HFMD population. STUDY DESIGN: The peptides encompassing the entire coding region of EV71 were chemically synthesized and displayed on a microarray...
June 3, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29886373/a-near-full-length-open-reading-frame-next-generation-sequencing-assay-for-genotyping-and-identification-of-resistance-associated-variants-in-hepatitis-c-virus
#17
M S Pedersen, U Fahnøe, T A Hansen, A G Pedersen, H Jenssen, J Bukh, K Schønning
BACKGROUND: The current treatment options for hepatitis C virus (HCV), based on direct acting antivirals (DAA), are dependent on virus genotype and previous treatment experience. Treatment failures have been associated with detection of resistance-associated substitutions (RASs) in the DAA targets of HCV, the NS3, NS5A and NS5 B proteins. OBJECTIVE: To develop a next generation sequencing based method that provides genotype and detection of HCV NS3, NS5A, and NS5 B RASs without prior knowledge of sample genotype...
June 1, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29860111/course-of-hev-viremia-and-anti-hev-igm-igg-response-in-asymptomatic-blood-donors
#18
Christian Kraef, Christian Schlein, Jens Hiller, Dirk Westhölter, Ulrike Denzer, Thomas Horvatits, Sven Peine, Ansgar W Lohse, Marc Lütgehetmann, Susanne Polywka, Sven Pischke
BACKGROUND: Globally, an estimated 20 million Hepatitis E infections occur every year. The course of viremia and antibody response has been investigated in patients with symptomatic hepatitis E. However, the majority of HEV infections in industrialized countries take a subclinical course. OBJECTIVES: To investigate the course of HEV viremia and epitope specific anti-HEV IgM/IgG response in asymptomatic blood donors in order to understand the immune response and viral clearance in asymptomatic blood donors with HEV infections...
May 29, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29885620/low-zika-virus-seroprevalence-among-pregnant-women-in-north-central-nigeria-2016
#19
Philipp Mathé, Daniel Z Egah, Janis A Müller, Nathan Y Shehu, Emmanuel T Obishakin, David D Shwe, Victor C Pam, Mark O Okolo, Christopher Yilgwan, Simji S Gomerep, Jonas Fuchs, Ibrahim Abok, Kenneth I Onyedibe, Ewa J Olugbo, Samson E Isa, Salamatu S Machunga-Mambula, Caleb J Attah, Jan Münch, Stephen Oguche, Marcus Panning
BACKGROUND: Zika virus (ZIKV) has been known for decades in Africa but contemporary data is lacking at large. OBJECTIVES: To describe the seroepidemiology of ZIKV in North Central Nigeria. STUDY DESIGN: We performed a cross-sectional study at six health care facilities in North Central Nigeria from January to December 2016. Detection of ZIKV antibodies was done using an anti-ZIKV recombinant non-structural protein 1 (NS1)-based ELISA. A colorimetric assay to detect ZIKV neutralizing antibodies was used on ELISA reactive and randomly selected ELISA non-reactive samples...
May 26, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29883908/evaluation-of-seegene-allplex-respiratory-panel-1-kit-for-the-detection-of-influenza-virus-and-human-respiratory-syncytial-virus
#20
Laura Gimferrer, Cristina Andrés, Ariadna Rando, Maria Piñana, Maria Gema Codina, Maria Del Carmen Martin, Francisco Fuentes, Susana Rubio, Pilar Alcubilla, Tomàs Pumarola, Andrés Antón
BACKGROUND: Influenza (FLUV) and human respiratory syncytial (HRSV) viruses are etiological agents of respiratory infections that cause a significant morbidity and mortality worldwide. A rapid and accurate diagnosis of these respiratory viruses is essential for an appropriate patient management. Molecular tests are the best detection option due to their high sensitivity and specificity. Seegene's Allplex™ Respiratory Panel 1 (Allplex RP1) is a real-time one-step RT-PCR assay for the simultaneous detection of FLUAV, FLUBV, HRSV-A and HRSV-B...
May 25, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
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