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Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology

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https://www.readbyqxmd.com/read/28226272/automated-processing-extraction-and-detection-of-herpes-simplex-virus-types-1-and-2-a-comparative-evaluation-of-three-commercial-platforms-using-clinical-specimens
#1
Matthew J Binnicker, Mark J Espy, Brian Duresko, Cole Irish, Jay Mandrekar
BACKGROUND: Recently, automated platforms have been developed that can perform processing, extraction and testing for herpes simplex virus (HSV) nucleic acid on a single instrument. OBJECTIVES: In this study, we compared three commercially-available systems; Aptima(®)/Panther (Hologic, San Diego, CA), ARIES(®) (Luminex Corporation, Austin, TX), and cobas(®) 4800 (Roche Molecular Systems Inc, Pleasanton, CA) for the qualitative detection of HSV-1/2 in clinical samples...
February 14, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28226273/acute-respiratory-infections-among-returning-hajj-pilgrims-jordan-2014
#2
Mohammad Mousa Al-Abdallat, Brian Rha, Sultan Alqasrawi, Daniel C Payne, Ibrahim Iblan, Alison M Binder, Aktham Haddadin, Mohannad Al Nsour, Tarek Alsanouri, Jawad Mofleh, Brett Whitaker, Stephen L Lindstrom, Suxiang Tong, Sami Sheikh Ali, Rebecca Moritz Dahl, LaShondra Berman, Jing Zhang, Dean D Erdman, Susan I Gerber
BACKGROUND: The emergence of Middle East Respiratory Syndrome coronavirus (MERS-CoV) has prompted enhanced surveillance for respiratory infections among pilgrims returning from the Hajj, one of the largest annual mass gatherings in the world. OBJECTIVES: To describe the epidemiology and etiologies of respiratory illnesses among pilgrims returning to Jordan after the 2014 Hajj. STUDY DESIGN: Surveillance for respiratory illness among pilgrims returning to Jordan after the 2014 Hajj was conducted at sentinel health care facilities using epidemiologic surveys and molecular diagnostic testing of upper respiratory specimens for multiple respiratory pathogens, including MERS-CoV...
February 8, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28214758/comparative-analysis-of-viral-shedding-in-pediatric-and-adult-subjects-with-central-nervous-system-associated-enterovirus-infections-from-2013-to-2015-in-switzerland
#3
S Cordey, M Schibler, A G L'Huillier, N Wagner, A R Gonçalves, J Ambrosioni, S Asner, L Turin, K M Posfay-Barbe, L Kaiser
BACKGROUND: Several enterovirus (EV) genotypes can result in aseptic meningitis, but their routes of access to the central nervous system remain to be elucidated and may differ between the pediatric and adult populations. OBJECTIVE: To assess the pattern of viral shedding in pediatric and adult subjects with acute EV meningitis and to generate EV surveillance data for Switzerland. STUDY DESIGN: All pediatric and adult subjects admitted to the University Hospitals of Geneva with a diagnosis of EV meningitis between 2013 and 2015 were enrolled...
February 7, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28209395/highlights-from-the-2016-hiv-diagnostics-conference-the-new-landscape-of-hiv-testing-in-laboratories-public-health-programs-and-clinical-practice
#4
Laura G Wesolowski, Monica M Parker, Kevin P Delaney, S Michele Owen
The 2016 HIV Diagnostics Conference, held in Atlanta, Georgia, was attended by public health officials, laboratorians, HIV testing program managers, surveillance coordinators and industry representatives. The conference addressed test performance data, the implementation of new testing algorithms, quality assurance, and the application of new tests in a variety of settings. With regard to the recommended Centers for Disease Control and Prevention/Association of Public Health Laboratories HIV laboratory testing algorithm, the conference featured performance data, implementation challenges such as a lack of test options for the second and third steps, as well as data needs for new tests that may be used as part of the algorithm...
February 7, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28189935/isolation-of-hepatitis-e-virus-genotype-4-from-patients-with-acute-cryptogenic-hepatitis-in-korea
#5
Sook-Hyang Jeong, Byung-Joo Park, Yong-Hyun Kim, Yun Suk Choi, Hee-Seop Ahn, Sang-Hoon Han, In-Soo Choi
BACKGROUND: Autochthonous hepatitis E occurs sporadically in developed countries. The consumption of undercooked pork containing hepatitis E virus genotype 3 (HEV-3) or 4 (HEV-4) is the major risk factor for infection. The serological diagnostic kits currently used in hospitals sometimes produce false-negative or -positive results. Therefore, detection of both HEV RNA and antibodies to the virus is required for confirmative diagnosis of hepatitis E. OBJECTIVES: We aimed to detect HEV in serum samples from patients with cryptogenic hepatitis and to determine the origin of HEV...
February 6, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28189092/polyomavirus-bk-and-jc-in-individuals-with-chronic-kidney-failure-kidney-transplantation-and-healthy-controls
#6
Talita Castro, Maria Cristina Domingues Fink, Marilia Figueiredo, Paulo Henrique Braz-Silva, Cláudio Mendes Pannuti, Karem Lopez Ortega, Marina Gallottini
BACKGROUND: New clinical approaches to diagnose and monitor individuals with systemic diseases have been employed through the use of oral fluids. Polyomavirus BK (BKPyV) and JC (JCPyV) infect asymptomatically around 80% of general population worldwide remaining latent in the body. In case of immunosuppression, a replication can occur, leading to diseases. OBJECTIVE: The aim of this study was to detect and quantify BKPyV and JCPyV in oral fluids of individuals with chronic kidney failure (CKF), kidney transplantation (KT) and controls compared with their detection in blood and urine, traditionally used for this test...
February 6, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28189936/performance-of-hbsag-quantification-assays-for-detection-of-hepatitis-b-virus-genotypes-and-diagnostic-escape-variants-in-clinical-samples
#7
Vincent Thibault, Annabelle Servant-Delmas, Thoai Duong Ly, Anne-Marie Roque-Afonso, Syria Laperche
BACKGROUND: The impact of hepatitis B virus (HBV) genomic variability on the measurement of HBsAg level has been poorly evaluated. OBJECTIVE: This study was designed to compare the performance of all the available assays measuring HBsAg level in this setting. STUDY DESIGN: A large selection of wild type HBV genotypes (n=184) and HBsAg strains harboring mutations in the S gene (n=81) from clinical samples was studied with three HBsAg quantification assays: Architect HBsAg (Abbott), LiaisonXL Murex HBsAg Quant (DiaSorin) and the Elecsys HBsAgII (Roche)...
February 4, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28171829/prevalence-of-false-positive-hepatitis-c-antibody-results-national-health-and-nutrition-examination-study-nhanes-2007-2012
#8
Anne C Moorman, Jan Drobenuic, Saleem Kamili
BACKGROUND: Screening large numbers of persons in a population with low prevalence of a disease leads to many false-positives. However, populations with low HCV prevalence may sometimes be recommended for HCV screening, for instance patients or healthcare workers after a possible healthcare-related exposure. OBJECTIVES: We determined the percentage of true vs false-positive HCV antibody (anti-HCV) test results among 2007-2012 participants in the National Health and Nutrition Examination Study (NHANES), a nationally representative study with approximately 1% HCV infection prevalence, much lower than in groups typically recommended for HCV screening...
January 30, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28033514/infections-due-to-alphaherpesviruses-in-early-post-transplant-period-after-allogeneic-haematopoietic-stem-cell-transplantation-results-of-a-5-year-survey
#9
Maciej Przybylski, Anna Majewska, Tomasz Dzieciatkowski, Patrycja Rusicka, Grzegorz W Basak, Barbara Nasilowska-Adamska, Jaroslaw Bilinski, Wieslaw W Jedrzejczak, Marta Wroblewska, Kazimierz Halaburda, Grazyna Mlynarczyk, Agnieszka Tomaszewska
BACKGROUND: Infections caused by human α-herpesviruses usually have a benign course with recurrencies. However, they may become dangerous in immunocompromised hosts. In this case, molecular methods constitute a reliable diagnostic tool enabling rapid assessment of the efficacy of antiviral treatment strategies. OBJECTIVES: We estimated the frequency of alphaherpesviral DNAemia and the viral load during early post-transplantation period after alloHSCT; we also analyzed association of the DNAemia and chosen parameters of the patients...
December 21, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28033513/norovirus-in-feces-and-nasopharyngeal-swab-of-children-with-and-without-acute-gastroenteritis-symptoms-first-report-of-gi-5-in-brazil-and-gi-3-in-nasopharyngeal-swab
#10
Nathânia Dábilla, Tâmera Nunes Vieira Almeida, Anniely Carvalho Rebouças Oliveira, André Kipnis, Thairiny Neres Silva, Fabíola Souza Fiaccadori, Teresinha Teixeira de Sousa, Divina das Dôres de Paula Cardoso, Menira Souza
BACKGROUND: Noroviruses (NoVs) are an important cause of acute gastroenteritis (AGE), worldwide. OBJECTIVES: To evaluate the frequency, viral load and molecular profile of NoV in fecal and nasopharyngeal swab samples from hospitalized children, and to determine children's secretor status. STUDY DESIGN: From May 2014 to May 2015, 219 children were included in the study, 96 with gastroenteric symptoms and 123 without gastroenteric symptoms. All fecal and nasopharyngeal swab samples were screened by TaqMan RT-qPCR duplex (GI/GII NoV) and quality samples were characterized by genomic sequencing...
December 21, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27992790/association-between-high-risk-human-papillomavirus-infection-and-co-infection-with-candida-spp-and-trichomonas-vaginalis-in-women-with-cervical-premalignant-and-malignant-lesions
#11
Ishita Ghosh, Richard Muwonge, Srabani Mittal, Dipanwita Banerjee, Pratip Kundu, Ranajit Mandal, Jaydip Biswas, Partha Basu
BACKGROUND: Human papillomavirus (HPV) is the necessary cause of cervical cancer. Cervico-vaginal infection with pathogens like Chlamydia is a likely cofactor. The interactions between HPV, Trichomonas vaginalis (TV) and Candida spp. are less understood, though inflammation induced by these pathogens has been demonstrated to facilitate oncogenesis. OBJECTIVE: Our study aimed to evaluate the association between Candida spp. and TV co-infection with HPV in cervical oncogenesis...
December 12, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27987421/successful-oral-treatment-of-ganciclovir-resistant-cytomegalovirus-with-maribavir-in-the-context-of-primary-immunodeficiency-first-case-report-and-review
#12
Philip D Bright, Mark Gompels, Matthew Donati, Sarah Johnston
No abstract text is available yet for this article.
December 12, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27988420/aptima-hpv-assay-versus-hybrid-capture-%C3%A2-2-hpv-test-for-primary-cervical-cancer-screening-in-the-hpv-focal-trial
#13
Darrel A Cook, Laurie W Smith, Jennifer Law, Wendy Mei, Dirk J van Niekerk, Kathy Ceballos, Lovedeep Gondara, Eduardo L Franco, Andrew J Coldman, Gina S Ogilvie, Dan Jang, Max Chernesky, Mel Krajden
BACKGROUND: Cervical cancer screening programs are switching from Pap screening to high-risk HPV testing. OBJECTIVES: To compare the Aptima HPV Assay (AHPV) with the Hybrid Capture(®) 2 High-Risk HPV DNA Test(®) (HC2) for primary cervical screening. STUDY DESIGN: HPV FOCAL is a randomized trial comparing HC2 to liquid-based cytology (LBC) for screening women aged 25-65. AHPV and HC2 were compared at the baseline screen (n=3473). Genotyping was by the Aptima HPV 16 18/45 Genotype Assay...
December 11, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27987422/diagnostic-performance-of-serological-assays-for-anti-hbs-testing-results-from-a-quality-assessment-program
#14
Stijn Raven, Jeannine Hautvast, Jim van Steenbergen, Reinier Akkermans, Cas Weykamp, Francis Smits, Christian Hoebe, Ann Vossen
BACKGROUND: Post-vaccination testing after hepatitis B vaccination is indispensable to evaluate long-term immunological protection. Using a threshold level of antibodies against hepatitis B surface antigen (anti-HBs) to define serological protection, implies reproducible and valid measurements of different diagnostic assays. OBJECTIVES: In this study we assess the performance of currently used anti-HBs assays. STUDY DESIGN: In 2013, 45 laboratories participated in an external quality assessment program using pooled anti-HBs serum samples around the cutoff values 10IU/l and 100IU/l...
December 7, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28011413/a-systematic-review-and-meta-analysis-of-seroprevalence-of-varicella-zoster-virus-a-nationwide-population-based-study
#15
REVIEW
Omolbanin Amjadi, Alireza Rafiei, Mohammadreza Haghshenas, Reza Alizadeh Navaei, Reza Valadan, Zahra Hosseini-Khah, Akbar Hedayatizadeh Omran, Mohsen Arabi, Reza Jafari Shakib, Tahoora Mousavi, G Hossein Ashrafi
Varicella zoster virus (VZV) causes chicken pox as a primary infection following which it becomes latent in neurons. It may then reactivate to cause shingles (herpes zoster). Severity of lesions and VZV pathogenicity are depended on the host's immune response and variant in VZV Dr Athina Myrto ChioniIdentification of VZV seroprevalance rate in general population may lead to develop new health strategic managements such as vaccination. Therefore, we aimed to provide a systematic review of the seroprevalence of VZV infection among Iranian population and estimate age- and gender- specific prevalence of VZV...
December 6, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27984766/diagnostic-usefulness-of-dynamic-changes-of-cmv-specific-t-cell-responses-in-predicting-cmv-infections-in-hct-recipients
#16
Jiwon Jung, Hyun-Jung Lee, Sun-Mi Kim, Young-Ah Kang, Young-Shin Lee, Yong Pil Chong, Heungsup Sung, Sang-Oh Lee, Sang-Ho Choi, Yang Soo Kim, Jun Hee Woo, Jung-Hee Lee, Je-Hwan Lee, Kyoo-Hyung Lee, Sung-Han Kim
BACKGROUND: CMV-specific cell mediated immune responses before and after hematopoietic stem cell transplantation (HCT) can categorize patients as at high or low risk of CMV development. OBJECTIVES: We evaluated the usefulness of the CMV-specific T-cell ELISPOT assay for predicting the development of CMV infections after HCT in recipients with donor-positive and recipient-positive CMV serology (D+/R+ ). STUDY DESIGN: CMV pp65 and IE1-specific ELISPOT assays were performed before HCT (D0), and at 30 (D30) and 90 (D90) days after HCT...
November 30, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27984765/hpv-prevalence-in-women-attending-cervical-screening-in-rural-malawi-using-the-cartridge-based-xpert-%C3%A2-hpv-assay
#17
Heather A Cubie, David Morton, Edson Kawonga, Mike Mautanga, Ipyana Mwenitete, Ngari Teakle, Bagrey Ngwira, Hilary Walker, Graeme Walker, Savel Kafwafwa, Beatrice Kabota, Reynier Ter Haar, Christine Campbell
BACKGROUND AND OBJECTIVES: Early experience with Cepheid Xpert(®) HPV assay (Xpert(®) HPV) suggests that its quick turnaround time and ease of application might make it a relevant contender for routine use in low and middle income countries (LMICs). In the context of a cervical screening service in rural Malawi, we aimed to assess practicalities of local laboratory testing with Xpert(®) HPV and provide preliminary high-risk HPV (HR-HPV) prevalence data. STUDY DESIGN: Liquid-based cytology (LBC) specimens were collected from women attending cervical screening clinics in Nkhoma, Malawi...
November 30, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28183063/real-life-experience-with-direct-acting-antivirals-agents-against-hepatitis-c-infection-in-elderly-patients
#18
Iria Rodríguez-Osorio, Purificación Cid, Luis Morano, Ángeles Castro, Marta Suárez, Manuel Delgado, Luis Margusino, Héctor Meijide, Berta Pernas, Andrés Tabernilla, José D Pedreira, Álvaro Mena, Eva Poveda
BACKGROUND: New direct-acting antivirals agents (DAAs) are very safe and well tolerated. OBJECTIVES: The purpose of this study is to analyse the efficacy and safety of DAAs in elderly patients, who have co-morbidities and are on chronic medications. STUDY DESIGN: All HCV-infected patients over 65 years old in clinical follow-up at two Hospitals in Spain who initiated anti-HCV therapy were included (August 2012-October 2015). RESULTS: A total of 120 HCV mono-infected patients were recorded...
March 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28160729/comparison-of-emag%C3%A2-versus-nuclisens-%C3%A2-easymag-%C3%A2-performance-on-clinical-specimens
#19
Magali Garcia, Céline Chessa, Anne Bourgoin, Geneviève Giraudeau, Chloé Plouzeau, Gérard Agius, Nicolas Lévêque, Agnès Beby-Defaux
BACKGROUND: eMAG™ (bioMerieux) is a new nucleic acid extraction platform based on magnetic silica technology, like its predecessor, NucliSENS(®) easyMAG(®) (bioMerieux). Using the same reagents and disposables, eMAG™ adds further automation, allowing simultaneous extraction of 48 samples directly from primary tubes, and distribution of nucleic acid extracts on PCR strips or in tubes at the end of the extraction process. OBJECTIVE: To compare the performance of eMAG™ and easyMAG(®) on various clinical specimens...
March 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28160728/point-of-care-testing-poct-in-molecular-diagnostics-performance-evaluation-of-genexpert-hcv-rna-test-in-diagnosing-and-monitoring-of-hcv-infection
#20
Ekta Gupta, Pragya Agarwala, Guresh Kumar, Rakhi Maiwall, Shiv Kumar Sarin
BACKGROUND: Molecular testing at the point-of-care may turn out to be game changer for HCV diagnosis and treatment monitoring, through increased sensitivity, reduced turnaround time, and ease of performance. One such assay GeneXpert(®) has recently been released. OBJECTIVES: Comparative analysis between performances of GeneXpert(®) and Abbott HCV-RNA was done. STUDY DESIGN: 174 HCV infected patients were recruited and, one time plasma samples from 154 patients and repeated samples from 20 patients, obtained at specific treatment time-points (0, 4, 12 and 24) weeks were serially re-tested on Xpert(®)...
March 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
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