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Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology

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https://www.readbyqxmd.com/read/28342315/inclusions-or-bystanders-cmv-pcr-sensitivity-and-specificity-in-tissue-samples
#1
LETTER
John Burston, Sebastian van Hal, Sally Dubedat, Andie Lee
No abstract text is available yet for this article.
March 18, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28342316/comparison-of-cytomegalovirus-cmv-specific-neutralization-capacity-of-hyperimmunoglobulin-hig-versus-standard-intravenous-immunoglobulin-ivig-preparations-impact-of-cmv-igg-normalization
#2
Matthias Stefan Schampera, Katrin Schweinzer, Harald Abele, Karl Oliver Kagan, Reinhild Klein, Ingo Rettig, Gerhard Jahn, Klaus Hamprecht
BACKGROUND: Based on a non-randomized study of Nigro et al. (2005) the intravenous administration of hyperimmunoglobulins (HIGs) is applied frequently to women with primary CMV-infection as "off-label use" in Germany. OBJECTIVES: In order to describe their CMV-specific neutralization-capacity in vitro, we analyzed the HIG preparations Cytotect(®), and Cytogam(®) as well as the standard intravenous immunoglobulins (IVIG) Octagam(®), Gamunex(®), Kiovig(®). STUDY DESIGN: We performed short-term cell-free CMV neutralization assays (CFNT) and long-term cell-adapted neutralization-plaque-reduction assays (PRANT)...
March 16, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28334685/serum-ifn-%C3%AE-induced-protein-10-ip-10-as-a-biomarker-for-severity-of-acute-respiratory-infection-in-healthy-adults
#3
Mary S Hayney, Kelsey M Henriquez, Jodi H Barnet, Tola Ewers, Heather M Champion, Sean Flannery, Bruce Barrett
BACKGROUND: The inflammatory chemokine, interferon-gamma inducible protein of 10kDa (IP-10), is a biomarker associated with several conditions. OBJECTIVES: This study investigated serum concentrations of IP-10 in healthy individuals who developed acute respiratory infection (ARI). The hypothesis is that serum IP-10 concentrations correlate with ARI severity and detection of viral pathogens. STUDY DESIGN: Data come from a randomized controlled trial measuring the effects of mindfulness meditation or exercise on ARI (Clinical Trials ID: NCT01654289)...
March 16, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28324789/evaluation-of-the-abbott-realtime-hcv-genotype-ii-plus-ruo-plus-assay-with-reference-to-core-and-ns5b-sequencing
#4
Melanie A Mallory, Danijela Lucic, Mark T W Ebbert, Gavin A Cloherty, Dan Toolsie, David R Hillyard
BACKGROUND: HCV genotyping remains a critical tool for guiding initiation of therapy and selecting the most appropriate treatment regimen. Current commercial genotyping assays may have difficulty identifying 1a, 1b and genotype 6. OBJECTIVE: To evaluate the concordance for identifying 1a, 1b, and genotype 6 between two methods: the PLUS assay and core/NS5B sequencing. STUDY DESIGN: This study included 236 plasma and serum samples previously genotyped by core/NS5B sequencing...
March 9, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28319847/a-european-multicentre-study-on-the-comparison-of-hcv-viral-loads-between-veris-hcv-assay-and-cobas-%C3%A2-taqman-%C3%A2-hcv-test-and-realtime-hcv-assay
#5
Patrick Braun, Monica Drago, Diana Fanti, Hervé Fleury, Jörg Hofmann, Jacques Izopet, Sebastian Kühn, Alessandra Lombardi, Valeria Micheli, Karine Sauné, Pascale Trimoulet, Duncan Whittaker, Alain Artus, Daniel Rhodes
BACKGROUND: Beckman Coulter has developed the VERIS HCV Assay for use on the new fully automated DxN VERIS Molecular Diagnostic System(¥) for HCV viral load monitoring. OBJECTIVES: Evaluate the clinical performance of the new quantitative VERIS HCV Assay. STUDY DESIGN: Comparison was performed on 279 plasma specimens from HCV infected patients tested with the VERIS HCV Assay and COBAS(®) Ampliprep/COBAS(®) Taqman(®) HCV Test and 369 specimens tested with the VERIS HCV Assay and RealTime HCV Assay...
March 9, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28319846/presence-of-cytomegalovirus-in-urine-and-blood-of-pregnant-women-with-primary-infection-might-be-associated-with-fetal-infection
#6
Marie-Luce Delforge, Elena Costa, Françoise Brancart, Deborah Goldman, Isabel Montesinos, Siham Zaytouni, Arnaud Marchant, Catherine Donner
BACKGROUND: Cytomegalovirus (CMV) congenital infection can result from primary infection, reinfection or reactivation among pregnant women. The risk of vertical transmission is much higher in case of primary infection, and the transmission rate increases with gestational age. However there are still many questions about maternal markers that can predict whether the virus will be transmitted to the fetus. OBJECTIVES: To investigate the relationship between the presence and the quantity of CMV in urine and blood of women presenting a primary CMV infection during pregnancy and the presence of congenital infection in their offspring...
March 7, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28292653/co-circulating-serotypes-in-a-dengue-fever-outbreak-differential-hematological-profiles-and-phylogenetic-relationships-among-viruses
#7
Andreia Moreira Dos Santos Carmo, Rodrigo Buzinaro Suzuki, Aline Diniz Cabral, Renata Torres da Costa, Gabriela Pena Massari, Michele Marcondes Riquena, Helio Augusto Alves Fracasso, Andre Eterovic, Arlei Marcili, Márcia Aparecida Sperança
BACKGROUND: Dengue virus, represented by four distinct, genetically diverse serotypes, is the etiologic agent of asymptomatic to severe hemorrhagic diseases. The spatiotemporal dynamics of dengue serotypes and its association to specific diseases vary among the different regions worldwide. By 2007, and in São Paulo State, Brazil, dengue-case concentration in urban centers had changed to increased incidence in small- and medium-sized towns, the case of Marília. OBJECTIVES: The aim of this article was to distinguish dengue serotypes circulating during the 2007 Marília outbreak and define their association to demographic and hematological patient profiles, as well as the phylogenetic relationships among the different viruses...
March 6, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28259567/pitfalls-in-interpretation-of-ct-values-of-rt-pcr-in-children-with-acute-respiratory-tract-infections
#8
Jérôme O Wishaupt, Tjeerd van der Ploeg, Leo C Smeets, Ronald de Groot, Florens G A Versteegh, Nico G Hartwig
BACKGROUND: The relation between viral load and disease severity in childhood acute respiratory tract infections (ARI) is not fully understood. OBJECTIVES: To assess the clinical relevance of the relation between viral load, determined by cycle threshold (CT) value of real-time reverse transcription-polymerase chain reaction assays and disease severity in children with single- and multiple viral ARI. STUDY DESIGN: 582 children with ARI were prospectively followed and tested for 15 viruses...
February 24, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28342746/diagnosing-acute-hiv-infection-the-performance-of-quantitative-hiv-1-rna-testing-viral-load-in-the-2014-laboratory-testing-algorithm
#9
Hsiu Wu, Stephanie E Cohen, Emily Westheimer, Cynthia L Gay, Laura Hall, Charles Rose, Lisa B Hightow-Weidman, Severin Gose, Jie Fu, Philip J Peters
New recommendations for laboratory diagnosis of HIV infection in the United States were published in 2014. The updated testing algorithm includes a qualitative HIV-1 RNA assay to resolve discordant immunoassay results and to identify acute HIV-1 infection (AHI). The qualitative HIV-1 RNA assay is not widely available; therefore, we evaluated the performance of a more widely available quantitative HIV-1 RNA assay, viral load, for diagnosing AHI. We determined that quantitative viral loads consistently distinguished AHI from a false-positive immunoassay result...
February 15, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28209395/highlights-from-the-2016-hiv-diagnostics-conference-the-new-landscape-of-hiv-testing-in-laboratories-public-health-programs-and-clinical-practice
#10
Laura G Wesolowski, Monica M Parker, Kevin P Delaney, S Michele Owen
The 2016 HIV Diagnostics Conference, held in Atlanta, Georgia, was attended by public health officials, laboratorians, HIV testing program managers, surveillance coordinators and industry representatives. The conference addressed test performance data, the implementation of new testing algorithms, quality assurance, and the application of new tests in a variety of settings. With regard to the recommended Centers for Disease Control and Prevention/Association of Public Health Laboratories HIV laboratory testing algorithm, the conference featured performance data, implementation challenges such as a lack of test options for the second and third steps, as well as data needs for new tests that may be used as part of the algorithm...
February 7, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28259054/comparison-of-on-treatment-hcv-rna-during-direct-antiviral-therapy-using-two-different-cobas-taqman-hcv-assays
#11
Johannes Vermehren, Marc Bourlière, Stanislas Pol, Patrick Marcellin, Robert H Hyland, Deyuan Jiang, Diana M Brainard, Stefan Zeuzem, Tania M Welzel
BACKGROUND: Repeated measurements of hepatitis C virus (HCV) RNA levels during antiviral therapy are recommended to monitor treatment efficacy and adherence. Throughout most direct antiviral agent (DAA) approval studies, HCV RNA cutoffs and endpoints were established with the COBAS TaqMan assay for use with the High Pure System (HPS/CTM). Different assays used in clinical practice may yield different quantitative results and possibly impact treatment decisions. OBJECTIVES: The concordance of the fully-automated COBAS AmpliPrep/COBAS TaqMan assay (CAP/CTM) with HPS/CTM and its ability to predict response to DAA-treatment with ledipasvir/sofosbuvir was assessed in cirrhotic patients with HCV genotype-1-infection who had failed prior treatment with protease inhibitor-based regimens...
April 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28249234/evaluation-of-two-vidas-%C3%A2-prototypes-for-detecting-anti-hev-igg
#12
Florence Abravanel, Nadège Goutagny, Corinne Perret, Sébastien Lhomme, Françoise Vischi, Alexandre Aversenq, Aude Chapel, Nathalie Dehainault, Nadia Piga, Jacqueline Dupret-Carruel, Jacques Izopet
BACKGROUND: High performance anti-hepatitis E virus (HEV) IgG assays are crucial for epidemiology. OBJECTIVE: To evaluate the performance of 2 prototypes developed for the VIDAS(®) automatic system for detecting anti-HEV IgG, one based on the ORF2 antigen (ORF2 prototype) and the other on the ORF2 and ORF3 antigens (ORF2/3 prototype), with reference to the Wantai anti-HEV IgG assay. STUDY DESIGN: The sensitivity of each assay was determined by testing 113 blood samples, 63 from immunocompetent and 50 from immunocompromised patients, with a proven HEV infection defined by detecting HEV RNA...
April 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28242509/serum-cytokine-chemokine-profiles-in-patients-with-dengue-fever-df-and-dengue-hemorrhagic-fever-fhd-by-using-protein-array
#13
Renato Antonio Dos Santos Oliveira, Marli Tenório Cordeiro, Patrícia Muniz Mendes Freire de Moura, Paulo Neves Bapti Baptista Filho, Ulisses de Mendonça Braga-Neto, Ernesto Torres de Azevedo Marques, Laura Helena Vega Gonzales Gil
BACKGROUND: DENV infection can induce different clinical manifestations varying from mild forms to dengue fever (DF) or the severe hemorrhagic fever (DHF). Several factors are involved in the progression from DF to DHF. No marker is available to predict this progression. Such biomarker could allow a suitable medical care at the beginning of the infection, improving patient prognosis. OBJECTIVES: The aim of this study was to compare the serum expression levels of acute phase proteins in a well-established cohort of dengue fever (DF) and dengue hemorrhagic fever (DHF) patients, in order to individuate a prognostic marker of diseases severity...
April 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28231562/corrigendum-to-prevalence-of-human-papillomavirus-genotypes-and-mucosal-iga-anti-viral-responses-in-women-with-cervical-ectopy-j-clin-virol-47-2010-43-48
#14
Olga L Monroy, Cecilia Aguilar, Marcela Lizano, Fernando Cruz-Talonia, Rosa M Cruz, Leticia Rocha-Zavaleta
No abstract text is available yet for this article.
April 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28226273/acute-respiratory-infections-among-returning-hajj-pilgrims-jordan-2014
#15
Mohammad Mousa Al-Abdallat, Brian Rha, Sultan Alqasrawi, Daniel C Payne, Ibrahim Iblan, Alison M Binder, Aktham Haddadin, Mohannad Al Nsour, Tarek Alsanouri, Jawad Mofleh, Brett Whitaker, Stephen L Lindstrom, Suxiang Tong, Sami Sheikh Ali, Rebecca Moritz Dahl, LaShondra Berman, Jing Zhang, Dean D Erdman, Susan I Gerber
BACKGROUND: The emergence of Middle East Respiratory Syndrome coronavirus (MERS-CoV) has prompted enhanced surveillance for respiratory infections among pilgrims returning from the Hajj, one of the largest annual mass gatherings in the world. OBJECTIVES: To describe the epidemiology and etiologies of respiratory illnesses among pilgrims returning to Jordan after the 2014 Hajj. STUDY DESIGN: Surveillance for respiratory illness among pilgrims returning to Jordan after the 2014 Hajj was conducted at sentinel health care facilities using epidemiologic surveys and molecular diagnostic testing of upper respiratory specimens for multiple respiratory pathogens, including MERS-CoV...
April 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28226272/automated-processing-extraction-and-detection-of-herpes-simplex-virus-types-1-and-2-a-comparative-evaluation-of-three-commercial-platforms-using-clinical-specimens
#16
Matthew J Binnicker, Mark J Espy, Brian Duresko, Cole Irish, Jay Mandrekar
BACKGROUND: Recently, automated platforms have been developed that can perform processing, extraction and testing for herpes simplex virus (HSV) nucleic acid on a single instrument. OBJECTIVES: In this study, we compared three commercially-available systems; Aptima(®)/Panther (Hologic, San Diego, CA), ARIES(®) (Luminex Corporation, Austin, TX), and cobas(®) 4800 (Roche Molecular Systems Inc, Pleasanton, CA) for the qualitative detection of HSV-1/2 in clinical samples...
April 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28214758/comparative-analysis-of-viral-shedding-in-pediatric-and-adult-subjects-with-central-nervous-system-associated-enterovirus-infections-from-2013-to-2015-in-switzerland
#17
S Cordey, M Schibler, A G L'Huillier, N Wagner, A R Gonçalves, J Ambrosioni, S Asner, L Turin, K M Posfay-Barbe, L Kaiser
BACKGROUND: Several enterovirus (EV) genotypes can result in aseptic meningitis, but their routes of access to the central nervous system remain to be elucidated and may differ between the pediatric and adult populations. OBJECTIVE: To assess the pattern of viral shedding in pediatric and adult subjects with acute EV meningitis and to generate EV surveillance data for Switzerland. STUDY DESIGN: All pediatric and adult subjects admitted to the University Hospitals of Geneva with a diagnosis of EV meningitis between 2013 and 2015 were enrolled...
April 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28189936/performance-of-hbsag-quantification-assays-for-detection-of-hepatitis-b-virus-genotypes-and-diagnostic-escape-variants-in-clinical-samples
#18
Vincent Thibault, Annabelle Servant-Delmas, Thoai Duong Ly, Anne-Marie Roque-Afonso, Syria Laperche
BACKGROUND: The impact of hepatitis B virus (HBV) genomic variability on the measurement of HBsAg level has been poorly evaluated. OBJECTIVE: This study was designed to compare the performance of all the available assays measuring HBsAg level in this setting. STUDY DESIGN: A large selection of wild type HBV genotypes (n=184) and HBsAg strains harboring mutations in the S gene (n=81) from clinical samples was studied with three HBsAg quantification assays: Architect HBsAg (Abbott), LiaisonXL Murex HBsAg Quant (DiaSorin) and the Elecsys HBsAgII (Roche)...
April 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28189935/isolation-of-hepatitis-e-virus-genotype-4-from-patients-with-acute-cryptogenic-hepatitis-in-korea
#19
Sook-Hyang Jeong, Byung-Joo Park, Yong-Hyun Kim, Yun Suk Choi, Hee-Seop Ahn, Sang-Hoon Han, In-Soo Choi
BACKGROUND: Autochthonous hepatitis E occurs sporadically in developed countries. The consumption of undercooked pork containing hepatitis E virus genotype 3 (HEV-3) or 4 (HEV-4) is the major risk factor for infection. The serological diagnostic kits currently used in hospitals sometimes produce false-negative or -positive results. Therefore, detection of both HEV RNA and antibodies to the virus is required for confirmative diagnosis of hepatitis E. OBJECTIVES: We aimed to detect HEV in serum samples from patients with cryptogenic hepatitis and to determine the origin of HEV...
April 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28189092/polyomavirus-bk-and-jc-in-individuals-with-chronic-kidney-failure-kidney-transplantation-and-healthy-controls
#20
Talita Castro, Maria Cristina Domingues Fink, Marilia Figueiredo, Paulo Henrique Braz-Silva, Cláudio Mendes Pannuti, Karem Lopez Ortega, Marina Gallottini
BACKGROUND: New clinical approaches to diagnose and monitor individuals with systemic diseases have been employed through the use of oral fluids. Polyomavirus BK (BKPyV) and JC (JCPyV) infect asymptomatically around 80% of general population worldwide remaining latent in the body. In case of immunosuppression, a replication can occur, leading to diseases. OBJECTIVE: The aim of this study was to detect and quantify BKPyV and JCPyV in oral fluids of individuals with chronic kidney failure (CKF), kidney transplantation (KT) and controls compared with their detection in blood and urine, traditionally used for this test...
April 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
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