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Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology

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https://www.readbyqxmd.com/read/29353074/diagnostic-value-of-human-papillomavirus-hpv-16-and-hpv18-viral-loads-for-the-detection-of-high-grade-cervical-intraepithelial-neoplasia-cin2-in-a-cohort-of-african-women-living-with-hiv
#1
Michel Segondy, Jean Ngou, Helen Kelly, Tanvier Omar, Olga Goumbri-Lompo, Sylviane Doutre, Philippe Mayaud, Marie-Noelle Didelot
BACKGROUND: African women living with HIV (WLHIV) are at high risk of cervical cancer but rarely adequately screened. Better strategies enabling identification of WLHIV with high-grade cervical intraepithelial lesions (CIN2+) are required. OBJECTIVES: To investigate the diagnostic value of HPV16 and HPV18 viral loads in a cohort of African WLHIV. DESIGN: HPV16 and HPV18 viral loads were determined by quantitation of the E6 gene DNA by real-time PCR in cervical specimens collected at baseline and endline (16 months) from 245 African WLHIV positive for HPV16 or/and HPV18...
January 16, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29346085/performance-of-the-bioplex-2200-hiv-ag-ab-assay-for-identifying-acute-hiv-infection
#2
Susan H Eshleman, Estelle Piwowar-Manning, Mariya V Sivay, Barbara Debevec, Stephanie Veater, Laura McKinstry, Linda-Gail Bekker, Sharon Mannheimer, Robert M Grant, Margaret A Chesney, Thomas J Coates, Beryl A Koblin, Jessica M Fogel
BACKGROUND: Assays that detect HIV antigen (Ag) and antibody (Ab) can be used to screen for HIV infection. OBJECTIVES: To compare the performance of the BioPlex 2200 HIV Ag-Ab assay and two other Ag/Ab combination assays for detection of acute HIV infection. STUDY DESIGN: Samples were obtained from 24 individuals (18 from the US, 6 from South Africa); these individuals were classified as having acute infection based on the following criteria: positive qualitative RNA assay; two negative rapid tests; negative discriminatory test...
January 11, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29331844/assessing-the-risk-of-cmv-reactivation-and-reconstitution-of-antiviral-immune-response-post-bone-marrow-transplantation-by-the-quantiferon-cmv-assay-and-real-time-pcr
#3
Adalbert Krawczyk, Jessica Ackermann, Birgit Goitowski, Rudolf Trenschel, Markus Ditschkowski, Jörg Timm, Hellmut Ottinger, Dietrich W Beelen, Nico Grüner, Melanie Fiedler
BACKGROUND: CMV reactivation is a major cause of severe complications in allogeneic hematopoietic stem cell transplant (HSCT) recipients. The risk of CMV reactivation depends on the serostatus (+/-) of the donor (D) and recipient (R). The reconstitution of CMV-specific T-cell responses after transplantation is crucial for the control of CMV reactivation. OBJECTIVES: The study aimed to determine the cellular immune status correlating with protection from high-level CMV viremia (>5000 copies/ml) and disease...
January 9, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29331843/performance-of-genotypic-algorithms-for-predicting-tropism-for-hiv-1-crf01_ae-recombinant
#4
C Soulie, L Morand-Joubert, J Cottalorda, C Charpentier, P Bellecave, L Le Guen, S Yerly, B Montes, S Fafi-Kremer, J Dina, V Avettand-Fenoel, C Amiel, C Roussel, C Pallier, K Zafilaza, S Sayon, A Signori-Schmuck, A Mirand, M A Trabaud, S Berger, V Calvez, A G Marcelin
OBJECTIVES: There is no consensus about the performances of genotypic rules for predicting HIV-1 non-B subtype tropism. Three genotypic methods were compared for CRF01_AE HIV-1 tropism determination. METHODS: The V3 env region of 207 HIV-1 CRF01_AE and 178 B subtypes from 17 centers in France and 1 center in Switzerland was sequenced. Tropism was determined by Geno2Pheno algorithm with false positive rate (FPR) 5% or 10%, the 11/25 rule or the combined criteria of the 11/25, net charge rule and NXT/S mutations...
January 8, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29353073/serum-hbv-dna-plus-rna-shows-superiority-in-reflecting-the-activity-of-intrahepatic-cccdna-in-treatment-na%C3%A3-ve-hbv-infected-individuals
#5
Hongxin Huang, Jie Wang, Weijie Li, Ran Chen, Xiangmei Chen, Fengmin Zhang, Dongping Xu, Fengmin Lu
BACKGROUND: Both serum hepatitis B virus (HBV) DNA and RNA can reflect intrahepatic covalently closed circular DNA (cccDNA) activity. However, correlations among viral markers haven't been fully explored. OBJECTIVES: Here we investigated the correlations between serum HBV RNA and other viral markers in acute hepatitis B patients and treatment-naïve chronic HBV-infected individuals. STUDY DESIGN: The serum viral markers of 19 acute hepatitis B patients and 84 treatment-naïve chronic HBV-infected individuals at different infection stages were quantified...
January 6, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29306112/predictive-factors-of-spontaneous-cmv-dnaemia-clearance-in-kidney-transplantation
#6
Johan Noble, Philippe Gatault, Bénédicte Sautenet, Catherine Gaudy-Graffin, Agnes Beby-Defaux, Antoine Thierry, Marie Essig, Jean-Michel Halimi, Eliza Munteanu, Sophie Alain, Matthias Buchler
Cytomegalovirus (CMV) infection occurs frequently after solid organ transplantation. Therapeutic strategies, in particular when to start a curative treatment, has not yet been defined. The purpose of this study was to assess predictive factors associated with spontaneous clearance of CMV DNAemia in kidney transplant recipients. METHODS: All kidney recipients of a single center were recruited. Patients with at least one positive CMV DNAemia during the first year post transplantation were included in our analysis...
December 29, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29306770/development-of-a-new-enzyme-immunoassay-for-improved-detection-of-rotavirus-in-fecal-specimens-of-vaccinated-infants
#7
Houping Wang, Merry Liu, Ken Sugata, Yuhuan Wang, Jennifer Hull, Kimberly Foytich, Baoming Jiang
BACKGROUND: Group A rotavirus is the most common cause of acute diarrhea in young children worldwide. A simple and rapid enzyme immunoassay (EIA) has been commonly used to detect rotavirus infection and evaluate rotavirus vaccines. Currently licensed commercial EIA kits have low sensitivity. A more sensitive detection of rotavirus can improve rotavirus diagnostics and vaccine efficacy studies. OBJECTIVE: A biotin-avidin based sandwich EIA was developed and compared with commercial EIA kits for improved detection of viral shedding in fecal samples from infants who received human rotavirus vaccine Rotarix in Mexico...
December 26, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29278832/an-epidemic-surge-of-influenza-a-h3n2-virus-at-the-end-of-the-2016-2017-season-in-taiwan-with-an-increased-viral-genetic-heterogeneity
#8
Ji-Rong Yang, Shu-Zhen Hsu, Chuan-Yi Kuo, Hsiang-Yi Huang, Teng-Yung Huang, Hsiao-Chi Wang, Ming-Tsan Liu
BACKGROUND: The epidemic of the 2016-2017 influenza season in Taiwan started early with moderate activity and was predominated by the influenza A(H3N2) virus. However, the influenza activity increased dramatically during the late stage of the 2016-2017 season. OBJECTIVES: The genetic and antigenic characteristics of the influenza A(H3N2) virus circulating in Taiwan during the 2016-2017 season were investigated. The relationship between virus clades and the patients' 2016-2017 vaccination histories was determined...
December 22, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29291537/comparative-evaluation-of-the-certest-viasure-flu-a-b-rsv-real-time-rt-pcr-detection-kit-on-the-bd-max-system-versus-a-routine-in-house-assay-for-detection-of-influenza-a-and-b-virus-during-the-2016-17-influenza-season
#9
Thomas Vognbjerg Sydenham, Malene Bek-Thomsen, Signe Dalsgaard Andersen, Birte Kolmos, Ea Sofie Marmolin, Ramona Trebbien, Jens Kjølseth Møller
BACKGROUND: Diagnosing of influenza rapidly and accurately helps clinicians to initiate appropriate treatment options and isolation protocols. Unnecessary antimicrobial treatment and laboratory testing can also be reduced. Assess commercial alternatives to in-house assays that may not only reduce laboratory technician "hands on" time but also the laboratory turnaround time is of interest. OBJECTIVES: We evaluated the performance of the VIASURE Flu A, B & RSV Real Time RT-PCR Detection Kit (CerTest Biotec) for detecting Influenza A and B viruses...
December 21, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29289815/lack-of-zika-virus-antibody-response-in-confirmed-patients-in-non-endemic-countries
#10
LETTER
Yaniv Lustig, Ani Ioana Cotar, Cornelia S Ceianu, Concetta Castilletti, Hana Zelena, Elisa Burdino, Carla van Tienen, Tatjana Avsic, Emma Aarons, Chantal Reusken
No abstract text is available yet for this article.
December 21, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29289814/safety-and-acceptability-of-human-papillomavirus-testing-of-self-collected-specimens-a-methodologic-study-of-the-impact-of-collection-devices-and-hpv-assays-on-sensitivity-for-cervical-cancer-and-high-grade-lesions
#11
Maarit K Leinonen, Kristina Schee, Christine M Jonassen, A Kathrine Lie, Camilla F Nystrand, Anbjørg Rangberg, Ingegerd E Furre, Marzena J Johansson, Ameli Tropé, Katrine D Sjøborg, Philip E Castle, Mari Nygård
BACKGROUND: Comparative data on different self-collection methods is limited. OBJECTIVES: To assess the impact of hrHPV testing of two self-collection devices for detection of cervical carcinoma and high-grade lesions. STUDY DESIGN: Three hundred ten patients collected two cervicovaginal specimens using a brush (Evalyn®Brush) and a swab (FLOQSwabs™), and filled a questionnaire at home. Then, a physician at the clinic took a cervical specimen into PreservCyt® buffer for hrHPV testing and cytology...
December 21, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29328964/a-european-multicenter-study-on-the-analytical-performance-of-the-veris-hbv-assay
#12
Patrick Braun, Rafael Delgado, Monica Drago, Diana Fanti, Hervé Fleury, Jacques Izopet, Alessandra Lombardi, Alessandro Mancon, Maria Angeles Marcos, Karine Sauné, Siobhan O Shea, Alfredo Pérez-Rivilla, John Ramble, Pascale Trimoulet, Jordi Vila, Duncan Whittaker, Alain Artus, Daniel Rhodes
BACKGROUND: Hepatitis B viral load monitoring is an essential part of managing patients with chronic Hepatits B infection. Beckman Coulter has developed the VERIS HBV Assay for use on the fully automated Beckman Coulter DxN VERIS Molecular Diagnostics System.1 OBJECTIVES: To evaluate the analytical performance of the VERIS HBV Assay at multiple European virology laboratories. STUDY DESIGN: Precision, analytical sensitivity, negative sample performance, linearity and performance with major HBV genotypes/subtypes for the VERIS HBV Assay was evaluated...
December 19, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29257982/diagnostic-performances-of-the-xpert%C3%A2-flu-pcr-test-and-the-osom%C3%A2-immunochromatographic-rapid-test-for-influenza-a-and-b-virus-among-adult-patients-in-the-emergency-department
#13
Stephanie Antoniol, Nadhira Fidouh, Aiham Ghazali, Houria Ichou, Donia Bouzid, Philippe Kenway, Christophe Choquet, Benoit Visseaux, Enrique Casalino
BACKGROUND: New rapid influenza diagnostic tests (RIDT) are available but their clinical utility in adults has not been validated. OBJECTIVES: To evaluate the diagnostic performances of OSOM® Ultra Flu A&B a RIDT on viral strains of influenza A/B from the last epidemic season, and its feasibility by Emergency Department (ED) physicians and nurses. STUDY DESIGN: Of the 1099 patients admitted to the ED with suspected influenza, all having a nasopharyngeal swab tested by the Xpert® Flu PCR and then stored at -20 °C; 500 were selected at random and their samples were tested using the RIDT...
December 13, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29253834/performance-evaluation-of-the-aptima-hsv-1-and-2-assay-for-the-detection-of-hsv-in-cutaneous-and-mucocutaneous-lesion-specimens
#14
Soya S Sam, Angela M Caliendo, Jessica Ingersoll, Deborah Abdul-Ali, Colleen S Kraft
BACKGROUND: Timely and precise laboratory diagnosis of Herpes simplex viruses (HSV) is required to guide clinical management. OBJECTIVES: The study evaluated limit of detection (LOD) and performance characteristics of the Aptima HSV 1 & 2 assay in comparison to four assays. STUDY DESIGN: The multi-center study compared qualitative detection of HSV-1 and 2 by the Aptima HSV-1 and 2 assay (Hologic) to ELVIS culture, Lyra Direct (Quidel), AmpliVue (Quidel) and a laboratory developed test (LDT)...
December 13, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29268148/a-chip-based-rapid-genotyping-assay-to-discriminate-between-rhinovirus-species-a-b-and-c
#15
Brenda M Westerhuis, Maryse A Wiewel, Alexander Am Ende, Lonneke van der Linden, Sylvie M Koekkoek, Katja C Wolthers, Olfert Landt
BACKGROUND: Human rhinoviruses (RVs) are increasingly associated with severe disease of the respiratory tract. Multiple studies highlighted the clinical significance of different RV species; RV-C is linked to asthma exacerbations and increased disease severity in children, whereas RV-B seems to correlate with milder disease. OBJECTIVES: Current typing strategies for differentiation of RV species are time consuming and require extensive equipment. Here we present a novel genotyping tool to discriminate RV species A, B and C...
December 8, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29241150/assessment-of-the-roche-linear-array-hpv-genotyping-test-within-the-valgent-framework
#16
Lan Xu, Anja Oštrbenk, Mario Poljak, Marc Arbyn
BACKGROUND: Cervical cancer screening programs are switching from cytology-based screening to high-risk (hr) HPV testing. Only clinically validated tests should be used in clinical practice. OBJECTIVES: To assess the clinical performance of the Roche Linear Array HPV genotyping test (Linear Array) within the VALGENT-3 framework. STUDY DESIGN: The VALGENT framework is designed for comprehensive comparison and clinical validation of HPV tests that have limited to extended genotyping capacity...
December 6, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29227860/respiratory-syncytial-virus-in-brazilian-infants-ten-years-two-cohorts
#17
Elinara Wollmeister, Alfonso Eduardo Alvarez, Juliana Cristina Santiago Bastos, Fernando Augusto Lima Marson, José Dirceu Ribeiro, Emílio Carlos Elias Baracat, Clarice Weis Arns, Adriana Gut Lopes Riccetto
BACKGROUND: Each year, a considerable amount of children will experience at least one episode of acute viral bronchiolitis (AVB) during their first year of life. About 10% of them will be hospitalized, with significant physical and economic burdens. OBJECTIVES: To compare two cohorts of infants with AVB, from same region, in a ten-year interval, regarding epidemiologic factors and viral etiology. STUDY DESIGN: Cohorts: 142 (2004) and 172 (2014) infants at ages zero to 12 months; clinical diagnosis of AVB; medical care in hospital and genetic screening of nasopharyngeal secretion for respiratory viruses...
December 6, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29223126/hpev-3-predominated-among-parechovirus-a-positive-infants-during-an-outbreak-in-2013-2014-in-queensland-australia
#18
Donna McNeale, Claire Y T Wang, Katherine E Arden, Ian M Mackay
BACKGROUND: Parechoviruses (HPeV) are endemic seasonal pathogens detected from the respiratory tract, gut, blood and central nervous system (CNS) of children and adults, sometimes in conjunction with a range of acute illnesses. HPeV CNS infection may lead to neurodevelopmental sequelae, especially following infection by HPeV-3, hence screening and genotyping are important to inform epidemiology, aetiology and prognosis. OBJECTIVES: To identify and characterise HPeVs circulating during an outbreak between November 2013 and April 2014 in Queensland, Australia...
December 6, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29197712/radiotherapy-induced-reactivation-of-neurotrophic-human-herpes-viruses-overview-and-management
#19
REVIEW
Marigdalia K Ramirez-Fort, Jianying Zeng, Amir Feily, Luis A Ramirez-Pacheco, Joseph M Jenrette, David L Mayhew, Talal Syed, S Lewis Cooper, Craig Linden, Witney S Graybill, Lars E French, Christopher S Lange
PURPOSE: Infection by Human Herpes Viruses (HHV) types 1-3, are prevalent throughout the world. It is known that radiotherapy can reactivate HHVs, but it is unclear how and to what extent reactivations can interact with or affect radiotherapeutic efficacy, patient outcomes and mortality risk. Herein, we aim to summarize what is known about Herpes Simplex Virus (HSV)-1,2 and Varicella Zoster Virus (VZV) pathophysiology as it relates to tumor biology, radiotherapy, chemo-radiotherapy, diagnosis and management so as to optimize cancer treatment in the setting of active HHV infection...
November 22, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29172075/real-time-rt-pcr-for-mayaro-virus-detection-in-plasma-and-urine
#20
Jesse J Waggoner, Alejandra Rojas, Alisha Mohamed-Hadley, Yvalena Arévalo de Guillén, Benjamin A Pinsky
BACKGROUND: Mayaro virus (MAYV) causes an acute febrile illness which can be difficult to differentiate from dengue or chikungunya. MAYV RNA can be detected in plasma during the first 3-5days of illness, but only a single rRT-PCR has been fully evaluated in the literature. OBJECTIVES: To develop an rRT-PCR for MAYV and evaluate assay performance using human plasma and urine samples spiked with different MAYV strains. STUDY DESIGN: A MAYV rRT-PCR targeting a region of the 5'UTR and nsp1 gene was designed from the alignment of all complete-genome MAYV sequences to be compatible with existing laboratory protocols...
November 21, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
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