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Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology

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https://www.readbyqxmd.com/read/30240941/measurement-of-plasma-hiv-1-rna-below-the-limit-of-quantification-20-copies-ml-of-commercial-assays-with-the-integrase-hiv-rna-single-copy-assay
#1
N Margot, D Koontz, S McCallister, J W Mellors, C Callebaut
BACKGROUND: Plasma HIV-1 RNA (viral load, VL) is measured routinely in HIV-infected persons with FDA-approved commercially available assays such as the Cobas-TaqMan HIV-1 Assay v2.0. This assay provides quantification of viremia ≥20 copies/mL. More sensitive methods, able to quantify low-level persistent viremia below the detection limit of commercially available assays, are needed to assess the impact of current HIV cure strategies on viremia. OBJECTIVES: The novel integrase HIV-1 RNA single-copy assay (iSCA) was evaluated for measurement of low-level persistent viremia in clinical trial samples (n = 151) from subjects participating in Gilead HIV clinical research...
September 10, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/30223253/validation-of-euroarray-hpv-test-using-the-valgent-framework
#2
Jessica Viti, Mario Poljak, Anja Oštrbenk, Ramya Bhatia, Elia Alcañiz Boada, Alyssa M Cornall, Kate Cuschieri, Suzanne Garland, Lan Xu, Marc Arbyn
BACKGROUND: Robust clinical and analytical validation of human papillomavirus (HPV) tests is a pre-requisite for their use in cervical cancer screening given the transience of most high-risk HPV infections. OBJECTIVES: To evaluate the EUROArray HPV test (PCR-based full HPV genotyping test) using the international validation of the VALGENT framework, which offers an opportunity to determine analytical and clinical performance according to internationally accepted performance metrics...
September 10, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/30223252/comparative-performance-of-human-papillomavirus-messenger-rna-versus-dna-screening-tests-at-baseline-and-48-months-in-the-hpv-focal-trial
#3
Darrel A Cook, Laurie W Smith, Jennifer H Law, Wendy Mei, Lovedeep Gondara, Dirk J van Niekerk, Kathy M Ceballos, Dan Jang, Max Chernesky, Eduardo L Franco, Gina S Ogilvie, Andrew J Coldman, Mel Krajden
BACKGROUND: HPV FOCAL is a randomized trial comparing high-risk HPV [Hybrid Capture 2 (HC2)] vs. liquid-based cytology (LBC) for primary cervical screening. OBJECTIVE: The present study objective was to compare Aptima HPV (AHPV) and HC2 assay performance at the intervention arm baseline and 48 mo. screens in relation to the rates of cervical intraepithelial neoplasia (CIN) grade 2 or worse (CIN2+). STUDY DESIGN: Women enrolled after December 2010 (n = 3475) were screened at baseline with both AHPV and HC2 (AHPV was blinded)...
September 10, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/30237097/molecular-epidemiology-of-coxsackievirus-a6-circulating-in-hong-kong-reveals-common-neurological-manifestations-and-emergence-of-novel-recombinant-groups
#4
Susanna K P Lau, Pyrear S H Zhao, Siddharth Sridhar, Cyril C Y Yip, Kam Leng Aw-Yong, Elaine Y Y Chow, Kelvin C M Cheung, Rex W H Hui, Ryan Y H Leung, Yuki S K Lai, Alan K L Wu, Kelvin K W To, Patrick C Y Woo, Kwok-Yung Yuen
BACKGROUND: Coxsackievirus A6 (CV-A6) represents the predominant enterovirus serotype in Hong Kong, but its epidemiology in our population was unknown. OBJECTIVES: To examine the clinical and molecular epidemiology of CV-A6 and detect emerging recombinant strains in Hong Kong. STUDY DESIGN: Nasopharyngeal aspirates (NPAs) from patients with febrile or respiratory illness were subject to RT-PCR for CV-A6 and sequencing of 5'-NCR and VP1. CV-A6-positive samples were further subject to 2C and 3D gene sequencing...
September 8, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/30219747/genetic-diversity-of-hepatitis-c-virus-in-pakistan-using-next-generation-sequencing
#5
Sana Saleem, Amjad Ali, Bushra Khubaib, Madiha Akram, Zareen Fatima, Muhammad Idrees
BACKGROUND: In Pakistan, HCV disease is considered a major public health issue with about 10-17 million people suffering with this infection and rate is increasing every day without any hindrance. The currently available Pyrosequencing approach used to analyze complex viral genomes as it can determine minor variants. It is crucial to understand viral evolution and quasispecies diversity in complex viral strains. OBJECTIVES: To assess genetic diversity in patients with HCV using Next Generation Sequencing (NGS) and compare nucleotide diversity of genotype 3a with respect to other genotypes...
September 8, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/30218891/risk-stratification-of-cervical-disease-using-detection-of-human-papillomavirus-hpv-e4-protein-and-cellular-mcm-protein-in-clinical-liquid-based-cytology-samples
#6
Andrew Stevenson, Kim Kavanagh, Jiafeng Pan, Lynne Stevenson, Heather Griffin, John Doorbar, Evelyn Scott, Miriam Deeny, Kate Cuschieri, Sheila V Graham
BACKGROUND: While human papillomavirus (HPV) DNA testing offers high sensitivity for the detection of significant cervical disease, its specificity is suboptimal given the high prevalence of transient HPV infections (CIN1 or less). Biomarkers to identify those suffering from low grade disease from those with high grade disease could save healthcare costs and reduce patient anxiety. OBJECTIVE: The objective of the present work was to develop and test an immunohistochemistry (IHC)-based dual viral and cellular biomarker strategy which was applicable to liquid based cytology (LBC) samples...
August 31, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/30196012/high-risk-hpv-detection-and-associated-cervical-lesions-in-a-population-of-french-menopausal-women
#7
Naouel Tifaoui, Thierry Maudelonde, Jacques Combecal, Roselyne Vallo, Sylviane Doutre, Marie-Noëlle Didelot, Nicolas Nagot, Michel Segondy, Nathalie Boulle
BACKGROUND: With population ageing, post-menopausal women represent a new group to be considered in cervical cancer screening strategies, including the significance of High Risk (HR)-HPV detection. OBJECTIVES: A retrospective analysis was conducted in a cohort of 406 menopausal women attending routine gynaecological consultation at the Hospital of Montpellier (France). STUDY DESIGN: All women benefited from a cervical smear and HR-HPV detection using Hybrid Capture 2 (HC2) test...
August 31, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/30195090/diagnostic-performance-of-multiplex-pcr-on-pulmonary-samples-versus-nasopharyngeal-aspirates-in-community-acquired-severe-lower-respiratory-tract-infections
#8
Sylvie Robert, Claire Lhommet, Cécile Le Brun, Denis Garot, Annick Legras, Julie Mankikian, Alain Goudeau
BACKGROUND: PCR-based techniques for the diagnosis of community- acquired severe lower respiratory tract infections are becoming the standard of care. However, their relative ability to identify either atypical bacteria or viruses that cause LRTI from clinical samples from various sources is yet to be determined. OBJECTIVES AND STUDY DESIGN: The aim of our study was to compare the diagnostic yield of nasopharyngeal aspirates with that of pulmonary samples for the etiological diagnosis of severe acute lower respiratory tract infections by multiplex PCR...
August 29, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/30196013/the-association-between-human-papillomavirus-16-18-dna-load-and-e6-protein-expression-in-cervical-intraepithelial-neoplasia-and-cancer
#9
Zeni Wu, Lulu Yu, Xiaoqin Lei, Yu Qin, Xun Zhang, Wen Chen, Youlin Qiao
BACKGROUND: It has been reported that high HPV DNA load and elevated E6 protein expression correlate with cervical cancer, but no epidemiological study has been performed. OBJECTIVES: To assess the association between type-specific HPV DNA load and presence of E6 protein in cervical intraepithelial neoplasia and cancer. STUDY DESIGN: This study was a cross-sectional multicenter study performed between 2013 and 2017. A total of 1717 women (normal histopathology: n = 916; CIN1: n = 94; CIN2: n = 63; CIN3: n = 130; SCC: n = 474; adenocarcinoma: n = 40) were included...
August 28, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/30176404/ongoing-and-emerging-arbovirus-threats-in-europe
#10
REVIEW
Luisa Barzon
During the last decades, arboviruses that are endemic in Europe have expanded their geographic range and caused an increasing number of human outbreaks. These viruses include West Nile virus, which is expanding its area of circulation in central and southern Europe; Usutu virus, with increasing evidence of a role in human disease; tick-borne encephalitis virus, which is being detected in northern areas and at higher altitudes as a consequence of climate warming; Crimean-Congo hemorrhagic fever virus, which is endemic in Eastern Europe and the Middle East, but has been recently detected in Spain; other viruses, such as California encephalitis virus antigenic group, which circulate in northern and central Europe but whose relevance for human disease in largely unknown...
August 23, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/30170224/comparing-molecular-quantification-of-herpes-simplex-virus-hsv-in-cerebrospinal-fluid-csf-with-quantitative-structural-and-functional-disease-severity-in-patients-with-hsv-encephalitis-hsve-implications-for-improved-therapeutic-approaches
#11
Kacy A Ramirez, Asim F Choudhri, Anami Patel, Noel T Lenny, Rebecca E Thompson, Leslie Berkelhammer Greenberg, Nancy Clanton Watson, Mehmet Kocak, John P DeVincenzo
BACKGROUND: Herpes Simplex Virus encephalitis (HSVE) is a devastating disease of all ages. Rigorous studies correlating viral load with neuroradiological and clinical severity have not been performed, particularly in neonates. Understanding these relationships may improve therapies. OBJECTIVES: To correlate molecularly quantified HSV in cerebrospinal fluid (CSF) and disease severity. STUDY DESIGN: HSV loads (VL) were evaluated by real-time PCR from the CSF of 33 patients (20 neonates, 5 children, 8 adults) with HSVE...
August 18, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/30114677/characterization-of-human-parainfluenza-virus-3-circulating-in-israel-2012-2015
#12
Irina Jornist, Khitam Muhsen, Daniela Ram, Yaniv Lustig, Virginia Levy, Sara Orzitser, Roberto Azar, Merav Weil, Viki Indenbaum, Danit Sofer, Ella Mendelson, Michal Mandelboim, Musa Hindiyeh
BACKGROUND: Human parainfluenza virus 3 (hPIV-3) causes respiratory tract infection. OBJECTIVES: The objective of this study was to describe the epidemiology of hPIV-3 infection among hospitalized patients and characterize the circulating strains. STUDY DESIGN: A cross-sectional study was conducted using respiratory samples of 15,946 hospitalized patients with respiratory symptoms in 2012-2015 in Israel. All samples were subjected to q-PCR and q-RT-PCR to determine the presence of hPIV-3 and other respiratory viruses...
August 13, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/30114678/wu-polyomavirus-detected-in-children-with-severe-respiratory-failure
#13
Kazuhiro Uda, Chitose Koyama-Wakai, Kensuke Shoji, Noriyasu Iwase, Daisuke Motooka, Shota Nakamura, Isao Miyairi
BACKGROUND: WU polyomavirus (WUPyV) is a relatively new virus associated with respiratory infections. However, its role is unclear in children with severe respiratory failure. OBJECTIVES: We aimed to evaluate the characteristics of severe respiratory failure associated with WUPyV infection in children. STUDY DESIGN: We retrospectively reviewed cases of respiratory tract infection at a tertiary children's hospital in Japan and performed real-time polymerase chain reaction (PCR) for WUPyV using residual extracted nucleic acid samples taken from respiratory tract samples of pediatric patients primarily with respiratory failure...
August 9, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/30103162/history-of-matrix-genes-mutations-within-pcr-target-regions-among-circulating-influenza-h3n2-clades-over-ten-plus-years
#14
Kathleen A Stellrecht
BACKGROUND: Emerging influenza A/H3N2 clades have been associated with M1 gene mutations which affect the performance of commercial PCR assays. OBJECTIVES AND STUDY DESIGN: The evolution and prevalence of problematic M1 mutations, and their associated viral clades, were investigated. All European and USA isolates from the GISAID database with both HA and M1 sequences available, collected during the respiratory seasons from the Fall of 2007 through January of 2018, were analyzed...
August 8, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/30099145/hepatitis-e-infection-in-stem-cell-and-solid-organ-transplantpatients-a-cross-sectional-study-the-importance-of-hev-rna-screening-in-peri-transplant-period
#15
Ian Reekie, Dianne Irish, Samreen Ijaz, Thomas Fox, Tehmina Bharucha, Paul Griffiths, Douglas Thorburn, Mark Harber, Stephen MacKinnon, Mallika Sekhar
BACKGROUND: Hepatitis E Virus (HEV) is a common cause of acute viral hepatitis worldwide. Typically associated with a self-limiting illness, infection may persist in immunosuppressed populations with significant morbidity and mortality. Based on clinical data published world-wide, UK blood safety guidance recommends the universal screening for HEV RNA of blood donors and donors of tissue, organs and stem cells. OBJECTIVES: This cross-sectional study aimed to determine the point prevalence of HEV viraemia and clinical course of viraemic patients in the peri-transplant period in solid organ transplant (SOT) and haematopoietic stem cell transplant (HSCT) recipients transplanted over a 3-year period (2013-2015)...
July 29, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/30103163/cellular-dna-quantification-in-respiratory-samples-for-the-normalization-of-viral-load-a-real-need
#16
Antonio Piralla, Federica Giardina, Francesca Rovida, Giulia Campanini, Fausto Baldanti
BACKGROUND: Respiratory tract infections have an enormous social economic impact, with high incidence of hospitalization and high costs. Adequate specimen collection is the first crucial step for the correct diagnosis of viral respiratory infections. OBJECTIVES: The present retrospective study aimed: i) to verify the cell yield obtained from sampling the nasal respiratory tract using mid-turbinate flocked swabs; ii) to evaluate the normalization of viral load, based on cell number; and iii) to compare the kinetics of viral infection obtained with normalized vs non-normalized viral load...
July 27, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/30195193/valhudes-a-protocol-for-validation-of-human-papillomavirus-assays-and-collection-devices-for-hpv-testing-on-self-samples-and-urine-samples
#17
M Arbyn, E Peeters, I Benoy, D Vanden Broeck, J Bogers, P De Sutter, G Donders, W Tjalma, S Weyers, K Cuschieri, M Poljak, J Bonde, C Cocuzza, F H Zhao, S Van Keer, A Vorsters
BACK GROUND: Systematic reviews have concluded that hrHPV DNA testing using target-amplification tests is as accurate on vaginal self-samples as on clinician-taken specimens for the detection of cervical precancer. However, insufficient evidence is available for specific HPV assay/self-sample device combinations. OBJECTIVES: The VALHUDES protocol is designed as a diagnostic test accuracy study that aims to compare the clinical sensitivity and specificity of particular hrHPV assay(s) on vaginal self-samples and first-void-urine, collected in agreement with standardized protocols, with hrHPV testing on matched clinician-taken samples...
October 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/30189412/respiratory-syncytial-virus-testing-capabilities-and-practices-among-national-respiratory-and-enteric-virus-surveillance-system-laboratories-united-states-2016
#18
Kristen E Allen, Christina Chommanard, Amber K Haynes, Dean D Erdman, Susan I Gerber, Lindsay Kim
BACKGROUND: Laboratory tests to detect respiratory syncytial virus (RSV) vary in sensitivity and specificity. Diagnostic testing practices can impact RSV disease diagnosis and burden estimates. OBJECTIVES: We surveyed a sample of laboratories that participated in the National Respiratory and Enteric Virus Surveillance System (NREVSS) in 2015-2016 to understand RSV testing, diagnostic capabilities, and practices. STUDY DESIGN: We distributed surveys in fall 2016 to NREVSS laboratories using an internet survey platform...
October 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/30077957/quantification-of-total-hiv-1-dna-in-buffy-coat-cells-feasibility-and-potential-added-value-for-clinical-follow-up-of-hiv-1-infected-patients-on-art
#19
Virginie Mortier, Els Demecheleer, Delfien Staelens, Marlies Schauvliege, Kenny Dauwe, Sylvie Dinakis, Laura Hebberecht, Leen Vancoillie, Chris Verhofstede
BACKGROUND: Successfully treated HIV-1 infected patients have a sustained undetectable viral RNA load. In these cases the total HIV-1 DNA load may constitute a valuable tool to further follow the overall viral burden. The value of this marker outside of cure research has been rarely studied. OBJECTIVES: To develop a quantitative (q)PCR for total HIV-1 DNA quantification in buffy coat cells and to evaluate the value of this parameter in clinical follow-up. STUDY DESIGN: A qPCR using primers and a probe in the conserved HIV-1 LTR region was adapted for use on DNA extracted from buffy coat cells...
September 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/30075460/a-large-healthcare-associated-outbreak-of-hepatitis-c-virus-genotype-1a-in-a-clinic-in-korea
#20
Yoon-Seok Chung, Ju-Yeon Choi, Myung Guk Han, Kye Ryeong Park, Su-Jin Park, Hyerim Lee, Youngmee Jee, Chun Kang
BACKGROUND: In November 2015, reuse of needles and syringes in conjunction with an increase in cases of HCV at a clinic in Korea was reported and investigated by public health authorities. Patients who received injections at the clinic from the first time this infection control breach may have occurred in 2008 through 2015 when the practice was stopped were offered screening for HCV and other blood-borne pathogens such as HIV, HTLV, HBV, syphilis, and malaria. OBJECTIVES: The aim of this study was to assess whether an outbreak of hepatitis C had occurred among the potentially exposed clinic patients due to this infection control breach...
September 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
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