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Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology

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https://www.readbyqxmd.com/read/27902961/application-of-next-generation-sequencing-for-the-detection-of-human-viral-pathogens-in-clinical-specimens
#1
Jayme Parker, Jack Chen
BACKGROUND: Next generation sequencing (NGS) is a new technology that can be used for broad detection of infectious pathogens and is rapidly becoming an essential platform in clinical laboratories. It is not known how NGS will displace or enhance gold standard methodologies in infectious disease diagnosis. OBJECTIVES: To investigate the feasibility and application of NGS technology in public health laboratories and compare NGS technology with conventional methods...
November 22, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27893998/influenza-and-rhinovirus-viral-load-and-disease-severity-in-upper-respiratory-tract-infections
#2
Andrea Granados, Adriana Peci, Allison McGeer, Jonathan B Gubbay
BACKGROUND: The role of viral load in respiratory viral infection is unclear. It is proposed that the viral load of some, but not all respiratory viruses correlate with disease severity. OBJECTIVES: We aimed to determine if an association exists between viral loads among patients in ambulatory settings, compared to those requiring hospitalization/intensive care unit (ICU) admission with influenza A/H3N2, influenza B, or human rhinovirus (HRV); we also explored the impact of age, gender and co-detection of Streptococcus pneumoniae on patient setting...
November 22, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27886635/multiplex-pcr-reveals-that-viruses-are-more-frequent-than-bacteria-in-children-with-cystic-fibrosis
#3
Sílvia Miró-Cañís, Sílvia Capilla-Rubio, Laura Marzo-Checa, Dionisia Fontanals-Aymerich, Isabel Sanfeliu-Sala, Mateu Espasa-Soley, Oscar Asensio-de-la-Cruz
BACKGROUND: Cystic fibrosis is a degenerative disease characterized by progressive epithelial secretory gland dysfunction associated with repeated respiratory infections. Bacterial infections are very frequent in children with cystic fibrosis, but because rapid METHODS: for screening for the wide variety of potentially involved viruses were unavailable until recently, the frequency of viral presence is unknown. Multiplex PCR enables screening for many viruses involved in respiratory infections...
November 13, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27893999/ebola-virus-disease-an-update-on-current-prevention-and-management-strategies
#4
REVIEW
M A Trad, W Naughton, A Yeung, L Mazlin, M O'sullivan, N Gilroy, D A Fisher, R L Stuart
Ebola virus disease (EVD) is characterised by systemic viral replication, immuno-suppression, abnormal inflammatory responses, large volume fluid and electrolyte losses, and high mortality in under-resourced settings. There are various therapeutic strategies targeting EVD including vaccines utilizing different antigen delivery methods, antibody-based therapies and antiviral drugs. These therapies remain experimental, but received attention following their use particularly in cases treated outside West Africa during the 2014-15 outbreak, in which 20 (80%) out of 25 patients survived...
November 11, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27846440/evaluation-of-human-papillomavirus-detection-by-abbott-m2000-system-on-samples-collected-by-fta-elute%C3%A2-card-in-a-chinese-hiv-1-positive-population
#5
Yu Qin, Hongyun Zhang, Natalia Marlowe, Mandong Fei, Judy Yu, Xiaoqin Lei, Lulu Yu, Jia Zhang, Di Cao, Li Ma, Wen Chen
BACKGROUND: HIV+/AIDS women have an increased risk of developing into CIN and cervical cancer compared to the general population. Limited medical resource and the lack of AIDS relevant knowledge impair the coverage and efficiency of cervical cancer screening. OBJECTIVES: To compare the clinical performance of self-collected dry storage medium (FTA Elute card) and physician-collected PreservCyt medium in detection of high risk human papillomavirus (HR HPV) among HIV-1 positive population...
November 9, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27835758/association-of-cytologic-grade-of-anal-pap-smears-with-viral-loads-of-human-papillomavirus-types-16-18-and-52-detected-in-the-same-specimens-from-men-who-have-sex-with-men
#6
Utaiwan Utaipat, Sumalee Siriaunkgul, Taweewat Supindham, Pongpun Saokhieo, Butsayarat Chaidaeng, Antika Wongthanee, Jongkolnee Settakorn, Kornkanok Sukpan, Darin Ruanpeng, Natthapol Kosashunhanan, Nuntisa Chotirosniramit, Patcharaphan Sugandhavesa, Toshiyuki Miura, Suwat Chariyalertsak
BACKGROUND: Human papilloma virus (HPV) load has been linked to cellular abnormalities of the uterine cervix, and proposed as predictors of HPV persistence and progression of dysplasia to cervical cancer. However, the association of HPV viral load and anal dysplasia and cancer has not been as thoroughly investigated. OBJECTIVES: To examine the association of the viral loads of high-risk HPV types 16, 18, and 52, with the cytologic severity grading in anal-swab specimens of MSM with and without HIV-1 co-infection...
November 6, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27835759/zika-virus-epidemic-in-brazil-i-fatal-disease-in-adults-clinical-and-laboratorial-aspects
#7
Raimunda S S Azevedo, Marialva T Araujo, Arnaldo J Martins Filho, Consuelo S Oliveira, Bruno T D Nunes, Ana C R Cruz, Ana G P A C Nascimento, Rita C Medeiros, Cezar A M Caldas, Fernando C Araujo, Juarez A S Quaresma, Barbara C B Vasconcelos, Maria G L Queiroz, Elizabeth S Travassos da Rosa, Daniele F Henriques, Eliana V P Silva, Jannifer O Chiang, Lívia C Martins, Daniele B A Medeiros, Juliana A Lima, Márcio R T Nunes, Jedson F Cardoso, Sandro P Silva, Pei-Yong Shi, Robert B Tesh, Sueli G Rodrigues, Pedro F C Vasconcelos
BACKGROUND: Zika virus (ZIKV) was first detected in Brazil in May 2015 and the country experienced an explosive epidemic. However, recent studies indicate that the introduction of ZIKV occurred in late 2013. Cases of microcephaly and deaths associated with ZIKV infection were identified in Brazil in November, 2015. OBJECTIVES: To determine the etiology of three fatal adult cases. STUDY DESIGN: Here we report three fatal adult cases of ZIKV disease...
December 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27835760/performance-evaluation-of-a-rapid-molecular-diagnostic-multicode-based-sample-to-answer-assay-for-the-simultaneous-detection-of-influenza-a-b-and-respiratory-syncytial-viruses
#8
Jolanda J C Voermans, S Seven-Deniz, P L A Fraaij, Annemiek A van der Eijk, M P G Koopmans, Suzan D Pas
BACKGROUND: Clinical signs and symptoms of different airway pathogens are generally indistinguishable, making laboratory tests essential for clinical decisions regarding isolation and antiviral therapy. Immunochromatographic tests (ICT) and direct immunofluorescence assays (DFA) have lower sensitivities and specificities than molecular assays, but have the advantage of quick turnaround times and ease-of-use. OBJECTIVE: To evaluate the performance of a rapid molecular assay, ARIES FluA/B & RSV, using laboratory developed RT-PCR assays (LDA), ICT (BinaxNOW) and DFA...
November 3, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27835757/vaccine-strain-herpes-zoster-found-in-the-trigeminal-nerve-area-in-a-healthy-child-a-case-report
#9
Sayaka Iwasaki, Kouji Motokura, Yoshitaka Honda, Masamitsu Mikami, Daisuke Hata, Atsuko Hata
A previously healthy 2-year-old girl, vaccinated for varicella at 17 months, was admitted because of left-sided facial herpes zoster caused by vaccine-strain varicella-zoster virus (VZV). She recovered fully with no complication after intravenous treatment using acyclovir. Earlier reports have described that herpes zoster (HZ) rashes caused by vaccine-strain VZV tend to occur on the dermis corresponding to the skin area where the varicella vaccine was received. However, rashes appeared on this girl only in the trigeminal nerve area, which is unrelated to the vaccinated site...
November 3, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27833060/enterovirus-71-genotype-c-isolated-in-peru-between-2006-and-2009
#10
Jose L Huaman, Gladys Carrion, Julia S Ampuero, Victor Ocaña, V Alberto Laguna-Torres, Robert D Hontz
Enterovirus-71 (EV71) was first isolated in California, United States in 1969, belongs to the genus Enterovirus, family Picornaviridae. Although infection normally causes mild, often undiagnosed illness, it can cause central nervous system infections that could turn fatal. Based on VP1 gene analysis, EV71 has been classified into six separate genotypes. Although the molecular epidemiology of EV71 has been well described via studies originating from Asia and Europe, it is mostly unknown in South America. From our study, four EV71 isolates from Peru were characterized using phylogenetic methods to determine their relationship with known reference strains...
November 3, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27842264/high-level-jcpyv-viruria-after-kidney-transplantation-clinical-and-histopathological-findings
#11
Ilkka Helanterä, Hans H Hirsch, Eeva Auvinen, Laura Mannonen, Maaret Nummi, Marion Wernli, Fernanda Ortiz, Anne Räisänen-Sokolowski, Marko Lempinen, Irmeli Lautenschlager
BACKGROUND: The significance of JC polyomavirus (JCPyV) after kidney transplantation ranges from irrelevant to full-blown nephropathy or PML. OBJECTIVES: To investigate the clinical significance of high-level JCPyV viruria and JCPyV primary infections after kidney transplantation. STUDY DESIGN: JCPyV viruria was detected in routine screening by quantitative real-time PCR in 40/238 kidney transplant recipients and was high-level (>10(7) copies/ml) in 17 patients...
November 2, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27821277/comparison-of-different-approaches-to-quantitative-adenovirus-detection-in-stool-specimens-of-hematopoietic-stem-cell-transplant-recipients
#12
K Kosulin, S Dworzak, A Lawitschka, S Matthes-Leodolter, T Lion
BACKGROUND: Adenoviruses almost invariably proliferate in the gastrointestinal tract prior to dissemination, and critical threshold concentrations in stool correlate with the risk of viremia. Monitoring of adenovirus loads in stool may therefore be important for timely initiation of treatment in order to prevent invasive infection. OBJECTIVES: Comparison of a manual DNA extraction kit in combination with a validated in-house PCR assay with automated extraction on the NucliSENS-EasyMAG device coupled with the Adenovirus R-gene kit (bioMérieux) for quantitative adenovirus analysis in stool samples...
November 1, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27865174/maraviroc-in-addition-to-cart-during-primary-hiv-infection-results-from-main-randomized-clinical-trial-and-96-weeks-follow-up
#13
Marco Ripa, Manuela Pogliaghi, Stefania Chiappetta, Silvia Nozza, Alessandro Soria, Giacomo Coppalini, Cristina Rovelli, Giuseppe Tambussi
BACKGROUND: Multi-targeted treatment strategies including maraviroc (MVC) during Primary HIV Infection (PHI) may benefit from the immune-modulatory properties of this CCR5-inhibitor. OBJECTIVES: We conducted a proof-of-concept clinical trial aimed at assessing whether maraviroc in addition of a combination antiretroviral therapy (cART) initiated during PHI would improve immunological and virological parameters. STUDY DESIGN: The MAIN (Maraviroc in HIV Acute INfection) study was a randomized open-label clinical trial (EUDRACT number: 2008-007004-29) which enrolled 29 patients with PHI...
October 31, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27838494/screening-characterisation-and-prevention-of-hepatitis-b-virus-hbv-co-infection-in-hiv-positive-children-in-south-africa
#14
Pieter Jooste, Anriette van Zyl, Emily Adland, Samantha Daniels, Louise Hattingh, Alethea Brits, Susan Wareing, Dominique Goedhals, Katie Jeffery, Monique Andersson, Philip Goulder, Philippa C Matthews
BACKGROUND: In South Africa, the first HBV vaccine dose is administered at age 6 weeks, leaving a potential window for vertical transmission. Insights into HBV seroprevalence in the vulnerable HIV-infected group are important to drive improvements in surveillance, treatment and prevention. OBJECTIVES: We set out to implement a screening program for HBV among HIV-infected children and adolescents in Kimberley, South Africa. Our aims were to demonstrate that screening is feasible and sustainable, to establish the prevalence of HBV, to characterise the HBV cases we identified, and to inform discussion about the infant vaccination schedule...
October 31, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27816021/analytical-and-clinical-evaluation-of-the-abbott-realtime-hepatitis-b-sequencing-assay
#15
Hee Jae Huh, Ji-Youn Kim, Myoung-Keun Lee, Nam Yong Lee, Jong-Won Kim, Chang-Seok Ki
BACKGROUND: Long-term nucleoside analogue (NA) treatment leads to selection for drug-resistant mutations in patients undergoing hepatitis B virus (HBV) therapy. The Abbott RealTime HBV Sequencing assay (Abbott assay; Abbott Molecular Inc., Des Plaines, IL, USA) targets the reverse transcriptase region of the polymerase gene and as such has the ability to detect NA resistance-associated mutations in HBV. OBJECTIVES: We evaluated the analytical performance of the Abbott assay and compared its diagnostic performance to that of a laboratory-developed nested-PCR and sequencing method...
October 29, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27816020/human-papillomavirus-detection-in-fine-needle-aspiration-cytology-of-lymph-node-metastasis-of-head-and-neck-squamous-cell-cancer
#16
Robert P Takes, Johannes H A M Kaanders, Carla M L van Herpen, Matthias A W Merkx, Pieter J Slootweg, Willem J G Melchers
BACKGROUND: Currently, testing on HPV in oropharyngeal squamous cell carcinoma (OPSCC) is performed on histological material. However, in a certain percentage of the cases who present with lymph node metastases no primary tumor can be identified and only fine needle aspiration cytology (FNAC) is available for analysis. OBJECTIVES: Purpose of this study was to assess HPV status on FNAC and to validate it using histological material of the same patients. STUDY DESIGN: Patients with cervical metastasis from OPSCC or cancer of an unknown primary tumor (CUP), diagnosed between 2007 and 2012 were included...
October 27, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27816019/molecular-detection-of-human-parechovirus-in-under-five-year-old-children-with-gastroenteritis
#17
Massimiliano Bergallo, Ilaria Galliano, Paola Montanari, Martina Rosa Brusin, Clara Gabiano
BACKGROUND: Currently, RT-PCR is used widely and considered to be a convenient, useful, and powerful method for molecular diagnosis, to detect pathogens from clinical specimens. OBJECTIVES: In this work we describe the development of an in-house Real-time Taqman PCR assay for quantification of HPeV in stool specimens. STUDY DESIGNS: A total of 137 fecal specimens previously screened for rotavirus and adenovirus were tested for HPeV virus. RESULTS: A total of 11 out of 137 (8%) episodes of acute gastroenteritis were associated with HPeV genomic detection with median viral load 14678±28927 genomes/mg fecal specimens...
October 26, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27816018/sequence-analysis-of-the-first-c2-subgenogroup-strain-of-enterovirus-71-isolated-in-korea
#18
Jung-Ah Lee, Young Sil Yoon, Ji-Yeon Hyeon, Jung Sik Yoo, Sang-Won Lee, June-Woo Lee, Sang-Won Lee
BACKGROUND: Enterovirus 71 (EV71) is an important causative agent of hand-foot-and-mouth disease with severe neurological complications, which may lead to death in children. Large outbreaks caused by EV71 have frequently occurred in Asia-Pacific region. OBJECTIVES: In Korea, the outbreaks have been caused by EV71 subgenogroups C3, and C4. Only genogroup C, especially subgenogroup C1, C3, C4, and C5, has been detected by the national enterovirus surveillance system in Korea...
October 22, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27788480/oral-human-papillomavirus-infection-sexual-behaviors-and-risk-of-oral-squamous-cell-carcinoma-in-southeast-of-china-a-case-control-study
#19
Fa Chen, Lingjun Yan, Fengqiong Liu, Jiangfeng Huang, Fangping Liu, Junfeng Wu, Yu Qiu, Xiaoyan Zheng, Lin Cai, Lisong Lin, Baochang He
BACKGROUND: The causal association between human papillomavirus (HPV) and oral squamous cell carcinoma (OSCC) remains controversial. Most of previous studies did not consider the potential modification effect of sexual behaviors when evaluating the role of HPV infection in OSCC risk. OBJECTIVES: To explore the independent and joint effects of oral HPV infection and sexual behaviors on OSCC in Chinese population. STUDY DESIGN: A case-control study was conducted from September 2012 to September 2015 in Fujian, China...
October 22, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27780081/human-enterovirus-and-rhinovirus-infections-are-associated-with-otitis-media-in-a-prospective-birth-cohort-study
#20
Elina Seppälä, Saara Sillanpää, Noora Nurminen, Heini Huhtala, Jorma Toppari, Jorma Ilonen, Riitta Veijola, Mikael Knip, Markku Sipilä, Jussi Laranne, Sami Oikarinen, Heikki Hyöty
BACKGROUND: Human enteroviruses (HEVs) and rhinoviruses (HRVs) have been linked to acute otitis media (AOM). OBJECTIVES: The present study evaluates the aforementioned association in a birth cohort setting. STUDY DESIGN: The cohort included 286 healthy infants (191 boys) followed from birth up to the age of 2 years in the Type 1 Diabetes Prediction and Prevention study in Finland. Stool samples were collected monthly and analyzed for the presence of HRV and HEV RNA using RT-PCR...
October 20, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
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