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Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society

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https://www.readbyqxmd.com/read/30216108/scc-s2-facilitates-tumor-proliferation-and-invasion-via-activating-wnt-signaling-and-depressing-hippo-signaling-in-colorectal-cancer-cells-and-predicts-poor-prognosis-of-patients
#1
Chuanjia Yang, Weixue Xu, Xiangzhen Meng, Siqi Zhou, Minglu Zhang, Dongxu Cui
SCC-S2 overexpression has been implicated in several human cancers, its correlation with prognosis and the mechanism how it reserved biological roles are still uncertain. The current study demonstrated that, in 142 archived colorectal carcinoma (CRC) tissue samples, SCC-S2 expression was significantly correlated with higher histological grade ( p=0.001), tumor invasion ( p=0.001), advanced Dukes staging ( p=0.002), positive regional lymph node metastasis ( p=0.024), and poor overall survival ( p<0.001). MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and Transwell assays showed that SCC-S2 significantly promoted the proliferation and invasion...
September 14, 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/30205019/effect-of-polarization-and-chronic-inflammation-on-macrophage-expression-of-heparan-sulfate-proteoglycans-and-biosynthesis-enzymes
#2
Maarten Swart, Linda Troeberg
Heparan sulfate (HS) proteoglycans on immune cells have the ability to bind to and regulate the bioactivity more than 400 bioactive protein ligands, including many chemokines, cytokines, and growth factors. This makes them important regulators of the phenotype and behavior of immune cells. Here we review how HS biosynthesis in macrophages is regulated during polarization and in chronic inflammatory diseases such as rheumatoid arthritis, atherosclerosis, asthma, chronic obstructive pulmonary disease and obesity, by analyzing published micro-array data and mechanistic studies in this area...
September 11, 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/30199656/refined-immunochemical-characterization-in-healthy-dog-skin-of-the-epidermal-cornification-proteins-filaggrin-and-corneodesmosin
#3
Didier Pin, Valérie Pendaries, Sokhna Keita Alassane, Carine Froment, Nicolas Amalric, Marie-Christine Cadiergues, Guy Serre, Marek Haftek, Emilie Vidémont, Michel Simon
Filaggrin (FLG) and corneodesmosin (CDSN) are two key proteins of the human epidermis. FLG loss-of-function mutations are the strongest genetic risk factors for human atopic dermatitis. Studies of the epidermal distribution of canine FLG and CDSN are limited. Our aim was to better characterize the distribution of FLG and CDSN in canine skin. Using immunohistochemistry on beagle skin, we screened a series of monoclonal antibodies (mAbs) specific for human FLG and CDSN. The cross-reactive mAbs were further used using immunoelectron microscopy and Western blotting...
September 10, 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/30138043/three-dimensional-cellular-raman-analysis-evidence-of-highly-ordered-lipids-within-cell-nuclei
#4
Bhagavathi Ramamurthy, Stanley Cohen, Mark Canales, Frederick D Coffman
Striking levels of spatial organization exist among and within interphase cell chromosomes, raising the possibility that other nuclear molecular components may also be organized in ways that facilitate nuclear function. To further examine molecular distributions and organization within cell nuclei, we utilized Raman spectroscopy to map distributions of molecular components, with a focus on cellular lipids. Although the vast majority of cellular lipids are associated with membranes, mapping the 2870/2850 cm-1 lipid peak ratios revealed that the most highly ordered lipids within interphase cells are found within cell nuclei...
August 23, 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/30133331/runx3-epigenetic-inactivation-is-associated-with-estrogen-receptor-positive-breast-cancer
#5
Hui Liu, Zhantao Yan, Qianqian Yin, Kai Cao, Yu Wei, Jaime Rodriguez-Canales, Dongshen Ma, Yongping Wu
The role of Runt-related transcription factor 3 ( RUNX3) gene in breast cancer remains not fully understood. We studied the correlation between RUNX3 gene promoter methylation and estrogen receptor (ER) expression status in breast cancer. Three breast cancer cell lines and 113 formalin-fixed, paraffin-embedded breast cancer tissue samples were analyzed for RUNX3 expression. Methylation-specific polymerase chain reaction was used to analyze RUNX3 methylation on the samples. Migration and invasion ability were evaluated in MCF7 cell line (RUNX3 methylated) treated with methylation inhibitor 5-Aza-2'-deoxycytidine (5-Aza-CdR) to study the effect of RUNX3 methylation status...
August 22, 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/30113872/expression-of-noncollagenous-bone-matrix-proteins-in-osteoblasts-stimulated-by-mechanical-stretching-in-the-cranial-suture-of-neonatal-mice
#6
Mika Ikegame, Sadakazu Ejiri, Hirohiko Okamura
We investigated the influence of mechanical stretching on the genetic expression pattern of noncollagenous bone matrix proteins in osteoblasts. The cranial sutures of neonatal mice were subjected to ex vivo mechanical stretching. In the nonstretched control group, as osteoblast differentiation progressed, the successive genetic expression of bone sialoprotein (BSP), osteopontin (OPN), and osteocalcin (OCN) was detected using in situ hybridization, in that order. In the stretched group, the sutures were widened, and after 24 hr of cultivation, a large number of osteoblasts and abundant new osteoid were observed on the borders of the parietal bones...
August 16, 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/30106598/4-6-diamidino-2-phenylindole-distinctly-labels-tau-deposits
#7
Chengyu Li, Tetsuya Takahashi, Tejashwi Shrestha, Eiji Kinoshita, Tomoyasu Matsubara, Masayasu Matsumoto, Hirofumi Maruyama
Tau deposits have distinct biochemical characteristics and vary morphologically based on identification with tau antibodies and several chemical dyes. Here, we report 4',6-diamidino-2-phenylindole (DAPI)-positivity of tau deposits. Furthermore, we investigated the cause for this positivity. DAPI was positive in 3R/4R (3-repeat/4-repeat) tau deposits in Alzheimer's disease, myotonic dystrophy, and neurodegeneration with brain iron accumulation, and in 4R tau deposits in corticobasal degeneration, but negative in 4R tau deposits in frontotemporal dementia with parkinsonism-17 and progressive supranuclear palsy...
August 14, 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/30052477/characterization-of-fgd5-expression-in-primary-breast-cancers-and-lymph-node-metastases
#8
Marit Valla, Patricia G Mjønes, Monica J Engstrøm, Borgny Ytterhus, Diana L Bordin, Barbara van Loon, Lars A Akslen, Lars J Vatten, Signe Opdahl, Anna M Bofin
Faciogenital dysplasia 5 ( FGD5) amplification drives tumor cell proliferation, and is present in 9.5% of breast cancers. We describe FGD5 expression, assess associations between FGD5 amplification and FGD5 expression, and assess FGD5 expression in relation to proliferation and prognosis. FGD5 immunohistochemistry was done on primary tumors ( n=829) and lymph node metastases ( n=231) from a cohort of Norwegian patients. We explored associations between FGD5 amplification, FGD5 expression, and proliferation, and analyzed the prognostic value of FGD5 expression by estimating cumulative risks of death and hazard ratios (HRs)...
July 27, 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/30052474/expression-of-cathepsin-l-and-its-intrinsic-inhibitors-in-glomeruli-of-rats-with-puromycin-aminonucleoside-nephrosis
#9
Ayano Kubo, Isao Shirato, Teruo Hidaka, Miyuki Takagi, Yu Sasaki, Katsuhiko Asanuma, Kazumi Ishidoh, Yusuke Suzuki
Cathepsin L, a lysosomal cysteine proteinase, may have a key role in various biological and disease processes by intracellular and extracellular degradation of proteins. We examined the levels of cathepsin L and its intrinsic inhibitors in glomeruli of rats with puromycin aminonucleoside (PAN) nephrosis. In contrast to the weak levels of cathepsin L in normal glomeruli, on days 4 and 8, strong immunostaining was detected in almost all podocytes when proteinuria and pathological changes of the podocytes developed...
July 27, 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/30047826/the-first-postlesion-minutes-an-in-vivo-study-of-extravasation-and-perivascular-astrocytes-following-cerebral-lesions-in-various-experimental-mouse-models
#10
László Tóth, Dávid Szöllősi, Katalin Kis-Petik, István Adorján, Ferenc Erdélyi, Mihály Kálmán
The immediate alterations following lesions cannot be investigated by using fixed tissues. Here, we employed two-photon microscopy to study the alterations to the permeability of blood-brain barrier and to glio-vascular connections in vivo during the first minutes following cortical lesions in mice. Four models were used: (1) cryogenic lesion, (2) photodisruption using laser pulses, (3) photothrombosis, and (4) bilateral carotid ligation. Sulforhodamine101 was used for supravital labeling of astrocytes and dextran-bound fluorescein isothiocyanate for the assessment of extravasation...
July 26, 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/30004288/expression-of-luminal-progenitor-marker-cd117-in-the-human-breast-gland
#11
Jiyoung Kim, René Villadsen
CD117 is a putative marker of luminal progenitor cells in the human breast. However, so far mapping the expression pattern of CD117 within the normal gland has not been reported. Here, we examined the anatomical distribution of CD117-expressing cells in lobular and ductal structures by immunohistochemistry. The presence of CD117-positive luminal cells could be divided into three distinct patterns: (1) contiguous, with coherent positive cells and rare negative cells interspaced; (2) patched, with a roughly equal frequency of positive and negative cells distributed focally; or (3) scattered, with few or no positive cells in the structure...
July 1, 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/29985723/characterization-of-epidermal-lipoxygenase-expression-in-normal-human-skin-and-tissue-engineered-skin-substitutes
#12
Carolyne Simard-Bisson, Lorraine Andrée Parent, Véronique J Moulin, Bernard Fruteau de Laclos
Lipoxygenases (LOXs) are enzymes likely to be involved in corneocyte lipid envelope formation and skin barrier function. In humans, mutations in epidermis-type lipoxygenase 3 ( eLOX-3) and 12R-lipoxygenase ( 12R-LOX) genes are associated with autosomal recessive congenital ichthyosis (ARCI), whereas deletion of these genes in mice causes epidermal defects. LOXs also represent a matter of interest in psoriasis as well as in cancer research. However, their expression as well as the exact role of these enzymes in normal human skin have not been fully described...
July 1, 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/29969056/agitation-modules-flexible-means-to-accelerate-automated-freeze-substitution
#13
Siegfried Reipert, Helmuth Goldammer, Christine Richardson, Martin W Goldberg, Timothy J Hawkins, Elena Hollergschwandtner, Walter A Kaufmann, Sebastian Antreich, York-Dieter Stierhof
For ultrafast fixation of biological samples to avoid artifacts, high-pressure freezing (HPF) followed by freeze substitution (FS) is preferred over chemical fixation at room temperature. After HPF, samples are maintained at low temperature during dehydration and fixation, while avoiding damaging recrystallization. This is a notoriously slow process. McDonald and Webb demonstrated, in 2011, that sample agitation during FS dramatically reduces the necessary time. Then, in 2015, we (H.G. and S.R.) introduced an agitation module into the cryochamber of an automated FS unit and demonstrated that the preparation of algae could be shortened from days to a couple of hours...
July 1, 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/29969055/evaluation-of-lineage-changes-in-the-gastric-mucosa-following-infection-with-helicobacter-pylori-and-specified-intestinal-flora-in-ins-gas-mice
#14
Carolina Pinzon-Guzman, Anne R Meyer, Rachel Wise, Eunyoung Choi, Sureshkumar Muthupalani, Timothy C Wang, James G Fox, James R Goldenring
Gastric adenocarcinoma develops in metaplastic mucosa associated with Helicobacter pylori infection in the stomach. We have sought to evaluate the precise lineage changes in the stomachs of insulin-gastrin (INS-GAS) mice infected with H. pylori and/or intestinal flora (Altered Schaedler's Flora; ASF). Stomachs from groups infected with H. pylori contained progressive spasmolytic polypeptide-expressing metaplasia (SPEM) compared with germ-free and mice infected with ASF alone. The overall phenotype of the H...
July 1, 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/29737911/immunolocalization-of-advanced-glycation-end-products-mitogen-activated-protein-kinases-and-transforming-growth-factor-%C3%AE-smads-in-pelvic-organ-prolapse
#15
Antonella Vetuschi, Simona Pompili, Anna Gallone, Angela D'Alfonso, Maria Gabriella Carbone, Gaspare Carta, Claudio Festuccia, Eugenio Gaudio, Alessandro Colapietro, Roberta Sferra
Collagen and matrix metalloproteinases (MMP) play a pivotal role in the pathophysiology of Pelvic Organ Prolapse (POP) as a switch between type I and III collagen together with a simultaneous activation of MMPs have been observed in the vaginal wall. The aim of this study was to evaluate the Advanced Glycation End (AGE) products, ERK1/2 and transforming growth factor (TGF)-β/Smad pathway expression in muscularis propria in women with POP compared with control patients. We examined 20 patients with POP and 10 control patients treated for uterine fibromatosis...
September 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/29718759/increased-insulin-like-growth-factor-binding-protein-1-phosphorylation-in-decidualized-stromal-mesenchymal-cells-in-human-intrauterine-growth-restriction-placentas
#16
Sahil S Singal, Karen Nygard, Robert Gratton, Thomas Jansson, Madhulika B Gupta
Intrauterine growth restriction (IUGR) is often caused by placental insufficiency, which is believed to be associated with decreased delivery of oxygen and nutrients to the placental barrier. We recently reported that hypoxia and/or leucine deprivation triggered hyperphosphorylation of insulin-like growth factor binding protein-1 (IGFBP-1) in decidualized human immortalized endometrial stromal cells (HIESCs), resulting in decreased insulin-like growth factor-1 (IGF-1) bioactivity. To test the hypothesis that human IUGR is associated with increased decidual IGFBP-1 phosphorylation at discrete sites, we used IUGR and gestational age matched appropriate for gestational age (AGA) placentas ( n=5 each)...
September 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/29683384/quantification-of-the-extracellular-matrix-molecule-thrombospondin-1-and-its-pericellular-association-in-the-brain-using-a-semiautomated-computerized-approach
#17
Jessie R Liu, Michel Modo
The structure and functions of the extracellular matrix (ECM), its spatial distribution and pericellular association of ECM molecules remain poorly understood. Colocalization of ECM molecules with cell phenotypes through immunohistochemistry can provide crucial insights into their juxtacrine signaling role as well as their structural relevance to tissue architecture. As manual quantification of images introduces intra- and inter-user bias and is cumbersome for high-throughput approaches, we implemented an automated high-throughput method to quantify the spatial distribution and cellular association of one ECM molecule, thrombospondin 1 (TSP1) with two major cell phenotypes, neurons, and astrocytes...
September 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/29676651/immunohistochemical-localization-of-fam83h-during-fluorosis-induced-mouse-molar-development
#18
Guanghui Shi, Yanyan Zhou, Jing Guo, Zhongrui Yang, Yang Lu, Yaling Song, Jie Jia
The clinical and pathological features of fluorosis are similar to amelogenesis imperfecta (AI) caused by FAM83H mutations, suggesting that excess fluoride could have effects on the expression of Fam83h. Our previous study found that Fam83h was downregulated by fluorosis induction in ameloblasts; the purpose of this study was to underline the importance of understanding the relationship between fluoride administration and Fam83h expression in vivo. A total of 80 healthy female adult Kunming mice were randomly divided into control group or F group that induced the clinical features of fluorosis...
September 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/29624116/evaluation-of-congo-red-staining-in-degenerating-porcine-photoreceptors-in-vitro-protective-effects-by-structural-and-trophic-support
#19
Camilla Mohlin, Dick Delbro, Anders Kvanta, Kjell Johansson
Congo red (CR) is a histological stain used for the detection of extracellular amyloids mediating various neurodegenerative diseases. Given that damaged photoreceptors appear to degenerate similarly to other nerve cells, CR staining was evaluated in experimentally injured porcine retina. CR staining appeared mostly as discrete cytosolic deposits with no obvious plaque formation during the investigated time period. Increases of CR labeling coincided temporally with the known accumulation of mislocalized opsins and increases of cell death...
September 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/29672206/full-karyotype-interphase-cell-analysis
#20
Adi Baumgartner, Christy Ferlatte Hartshorne, Aris A Polyzos, Heinz-Ulrich G Weier, Jingly Fung Weier, Ben O'Brien
Aneuploidy seems to play not only a decisive role in embryonal development but also in tumorigenesis where chromosomal and genomic instability reflect a universal feature of malignant tumors. The cost of whole genome sequencing has fallen significantly, but it is still prohibitive for many institutions and clinical settings. No applied, cost-effective, and efficient technique has been introduced yet aiming at research to assess the ploidy status of all 24 different human chromosomes in interphases simultaneously, especially in single cells...
August 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
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