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Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society

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https://www.readbyqxmd.com/read/27881474/cancer-secretome-may-influence-bsp-and-dsp-expression-in-human-salivary-gland-cells
#1
Samantha Lynn Hamilton, Blake Ferando, Asha Sarah Eapen, Jennifer Chian Yu, Anita Rose Joy
One of the biggest challenges in managing head and neck cancers, especially salivary gland cancers, is the identification of secreted biomarkers of the disease that can be evaluated noninvasively. A relevant source of enriched tumor markers could potentially be found in the tumor secretome. Although numerous studies have evaluated secretomes from various cancers, the influence of the cancer secretome derived from salivary gland cancers on the behavior of normal cells has not yet been elucidated. Our data indicate that secretome derived from salivary gland cancer cells can influence the expression of two potential biomarkers of oral cancer-namely, bone sialoprotein (BSP) and dentin sialoprotein (DSP)-in normal salivary gland cells...
November 23, 2016: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/27879410/triple-staining-including-foxa2-identifies-stem-cell-lineages-undergoing-hepatic-and-biliary-differentiation-in-cirrhotic-human-liver
#2
Charles E Rogler, Remon Bebawee, Joe Matarlo, Joseph Locker, Nicole Pattamanuch, Sanjeev Gupta, Leslie E Rogler
Recent investigations have reported many markers associated with human liver stem/progenitor cells, "oval cells," and identified "niches" in diseased livers where stem cells occur. However, there has remained a need to identify entire lineages of stem cells as they differentiate into bile ducts or hepatocytes. We have used combined immunohistochemical staining for a marker of hepatic commitment and specification (FOXA2 [Forkhead box A2]), hepatocyte maturation (Albumin and HepPar1), and features of bile ducts (CK19 [cytokeratin 19]) to identify lineages of stem cells differentiating toward the hepatocytic or bile ductular compartments of end-stage cirrhotic human liver...
November 22, 2016: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/27872404/unique-cellular-lineage-composition-of-the-first-gland-of-the-mouse-gastric-corpus
#3
Andrew O'Neil, Christine P Petersen, Eunyoung Choi, Amy C Engevik, James R Goldenring
The glandular stomach has two major zones: the acid secreting corpus and the gastrin cell-containing antrum. Nevertheless, a single gland lies at the transition between the forestomach and corpus in the mouse stomach. We have sought to define the lineages that make up this gland unit at the squamocolumnar junction. The first gland in mice showed a notable absence of characteristic corpus lineages, including parietal cells and chief cells. In contrast, the gland showed strong staining of Griffonia simplicifolia-II (GSII)-lectin-positive mucous cells at the bases of glands, which were also positive for CD44 variant 9 and Clusterin...
November 21, 2016: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/27856824/all-trans-retinoic-acid-augments-the-histopathological-outcome-of-neuroinflammation-and-neurodegeneration-in-lupus-prone-mrl-lpr-mice
#4
Michelle H Theus, Joshua B Sparks, Xiaofeng Liao, Jingjing Ren, Xin M Luo
Recently, we demonstrated that treatment with all-trans-retinoic acid (tRA) induced a paradoxical effect on immune activation during the development of autoimmune lupus. Here, we further describe its negative effects on mediating neuroinflammation and neurodegeneration. Female MRL/lpr mice were orally administered tRA or VARA (retinol mixed with 10% tRA) from 6 to 14 weeks of age. Both treatments had a significant effect on brain weight, which correlated with histopathological evidence of focal astrogliosis, meningitis, and ventriculitis...
November 16, 2016: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/27789731/evaluation-of-akt-and-rictor-expression-levels-in-astrocytomas-of-all-grades
#5
Arthur William Alvarenga, Luis Eduardo Machado, Bruna Roz Rodrigues, Fernanda Cristina Sulla Lupinacci, Paulo Sanemastu, Eduardo Matta, Martín Roffé, Luís Fernando Bleggi Torres, Isabela Werneck da Cunha, Vilma Regina Martins, Glaucia Noeli Maroso Hajj
The mammalian target of rapamycin (mTOR) binds to several protein partners and forms two complexes, termed mTOR complexes 1 and 2 (mTORC1/2), that differ in components, substrates, and regulation. mTORC2 contains the protein Rapamycin-insensitive companion of mTOR (RICTOR); phosphorylates kinases of the AGC family, such as Akt; and controls the cytoskeleton. Even though the regulation of mTORC2 activity remains poorly understood, the hyperactivation of this signaling pathway has been shown to contribute to the oncogenic properties of gliomas in experimental models...
October 27, 2016: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/27798289/antigen-masking-during-fixation-and-embedding-dissected
#6
Carla Rossana Scalia, Giovanna Boi, Maddalena Maria Bolognesi, Lorella Riva, Marco Manzoni, Linde DeSmedt, Francesca Maria Bosisio, Susanna Ronchi, Biagio Eugenio Leone, Giorgio Cattoretti
Antigen masking in routinely processed tissue is a poorly understood process caused by multiple factors. We sought to dissect the effect on antigenicity of each step of processing by using frozen sections as proxies of the whole tissue. An equivalent extent of antigen masking occurs across variable fixation times at room temperature. Most antigens benefit from longer fixation times (>24 hr) for optimal detection after antigen retrieval (AR; for example, Ki-67, bcl-2, ER). The transfer to a graded alcohol series results in an enhanced staining effect, reproduced by treating the sections with detergents, possibly because of a better access of the polymeric immunohistochemical detection system to tissue structures...
October 20, 2016: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/27680670/differential-lectin-binding-patterns-identify-distinct-heart-regions-in-giant-danio-devario-aequipinnatus-and-zebrafish-danio-rerio-hearts
#7
Trina Manalo, Adam May, Joshua Quinn, Dominique S Lafontant, Olubusola Shifatu, Wei He, Juan M Gonzalez-Rosa, Geoffrey C Burns, Caroline E Burns, Alan R Burns, Pascal J Lafontant
Lectins are carbohydrate-binding proteins commonly used as biochemical and histochemical tools to study glycoconjugate (glycoproteins, glycolipids) expression patterns in cells, tissues, including mammalian hearts. However, lectins have received little attention in zebrafish (Danio rerio) and giant danio (Devario aequipinnatus) heart studies. Here, we sought to determine the binding patterns of six commonly used lectins-wheat germ agglutinin (WGA), Ulex europaeus agglutinin, Bandeiraea simplicifolia lectin (BS lectin), concanavalin A (Con A), Ricinus communis agglutinin I (RCA I), and Lycopersicon esculentum agglutinin (tomato lectin)-in these hearts...
September 28, 2016: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/27680669/nucleolar-reorganization-upon-site-specific-double-strand-break-induction-dna-repair-and-epigenetics-of-ribosomal-genes
#8
Michal Franek, Alena Kovaříková, Eva Bártová, Stanislav Kozubek
DNA damage response (DDR) in ribosomal genes and mechanisms of DNA repair in embryonic stem cells (ESCs) are less explored nuclear events. DDR in ESCs should be unique due to their high proliferation rate, expression of pluripotency factors, and specific chromatin signature. Given short population doubling time and fast progress through G1 phase, ESCs require a sustained production of rRNA, which leads to the formation of large and prominent nucleoli. Although transcription of rRNA in the nucleolus is relatively well understood, little is known about DDR in this nuclear compartment...
September 28, 2016: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/27680668/sialidase-unmasks-mucin-domain-epitopes-of-lubricin
#9
Kathryn A Solka, Ira J Miller, Thomas M Schmid
Lubricin is a secreted, mucin-like glycoprotein and proteoglycan abundant in synovial fluid that provides boundary lubrication and prevents cell adhesion in synovial joints. The antilubricin S6.79 monoclonal antibody recognizes an O-linked glycopeptide epitope in lubricin's mucin domain. The central, long mucin domain of lubricin is extensively O-glycosylated with Gal(β1-3)GalNAc-O-Ser/Thr, and about two thirds of the O-glycosylated sites are capped with sialic acid. Our aim was to determine whether removal of sialic acid by sialidase could improve the detection of lubricin in a number of human tissues using the S6...
September 28, 2016: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/27680667/immunophenotyping-of-rabbit-testicular-germ-and-sertoli-cells-across-maturational-stages
#10
B Banco, G Grilli, C Giudice, A Tomas Marques, S Cotti Cometti, G Visigalli, V Grieco
During testicular maturation, both Sertoli cells (SCs) and germ cells (GCs) switch from an immature to a mature immunophenotype. The reexpression of markers of immaturity in adults has been reported in cancer and in other testicular pathologies, in men as well as in animal species. Naturally affected with testicular cancer, rabbits have long been used in human reproductive research, but reports on the expression of testicular cell markers in this species are few and data about the immunophenotype of normal postnatal SCs and GCs are lacking...
September 28, 2016: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/27837159/variability-in-platelet-derived-growth-factor-receptor-alpha-antibody-specificity-may-impact-clinical-utility-of-immunohistochemistry-assays
#11
Timothy R Holzer, Leslie O'Neill Reising, Kelly M Credille, Andrew E Schade, Gerard J Oakley
Aberrant regulation of the receptor tyrosine kinase platelet-derived growth factor alpha (PDGFRα) is implicated in several types of cancer. Inhibition of the PDGFRα pathway may be a beneficial therapy, and detection of PDGFRα in tumor biopsies may lead to insights about which patients respond to therapy. Exploratory or clinical biomarker use of PDGFRα IHC has been frequently reported, often with polyclonal antibody sc-338. An sc-338-based assay was systematically compared with anti-PDGFRα rabbit monoclonal antibody D13C6 using immunoblot profiling and IHC in formalin-fixed and paraffin-embedded human tumor cell lines...
December 2016: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/27798288/cell-line-macroarray-an-alternative-high-throughput-platform-to-analyze-hipsc-lines
#12
Alberto La Spada, Simona Baronchelli, Linda Ottoboni, Francesca Ruffini, Gianvito Martino, Nunzia Convertino, Aikaterini Ntai, Tobias Steiner, Ida Biunno, Andrea De Blasio
In the past decade, tissue microarray (TMA) technology has evolved as an innovative tool for high-throughput proteomics analysis and mainly for biomarker validation. Similarly, enormous amount of data can be obtained from the cell line macroarray (CLMA) technology, which developed from the TMA using formalin-fixed, paraffin-embedded cell pellets. Here, we applied CLMA technology in stem cell research and in particular to identify bona fide neogenerated human induced pluripotent stem cell (hiPSC) clones suitable for down the line differentiation...
December 2016: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/27798287/altered-t-cell-balance-in-lymphoid-organs-of-a-mouse-model-of-colorectal-cancer
#13
Scott M Tanner, Joseph G Daft, Stephanie A Hill, Colin A Martin, Robin G Lorenz
The adenomatous polyposis coli (APC) gene is a known tumor suppressor gene, and mice with mutations in Apc (Apc(Min/+)) spontaneously form multiple intestinal neoplasms. In this model of human colorectal cancer (CRC), it has been reported that CD4(+) T-cell-derived interleukin 17 (IL-17) promotes intestinal tumor development, but it is not known if the Apc mutation actually directly alters T-cell function and subsequently tumor immunosurveillance. To investigate the Apc(Min/+) mutation on T-cell function, flow cytometric, histochemical, and immunofluorescent studies on both wild-type (Apc(+/+)) and Apc(Min/+) mice were performed...
December 2016: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/27798286/megalin-is-predominantly-observed-in-vesicular-structures-in-first-and-third-trimester-cytotrophoblasts-of-the-human-placenta
#14
Tina Storm, Erik I Christensen, Julie Nelly Christensen, Tine Kjaergaard, Niels Uldbjerg, Agnete Larsen, Bent Honoré, Mette Madsen
The membrane receptor megalin is crucial for normal fetal development. Besides its expression in the developing fetus, megalin is also expressed in the human placenta. Similar to its established function in the kidney proximal tubules, placental megalin has been proposed to mediate uptake of vital nutrients. However, details of megalin expression, subcellular localization, and function in the human placenta remain to be established. By immunohistochemical analyses of first trimester and term human placenta, we showed that megalin is predominantly expressed in cytotrophoblasts, the highly proliferative cells in placenta...
December 2016: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/27666430/matrix-metalloproteinase-20-co-expression-with-dentin-sialophosphoprotein-in-human-and-monkey-kidneys
#15
Kalu U E Ogbureke, Komal Koli, Geetu Saxena
We recently reported the expression of matrix metalloproteinase 20 (MMP20), hitherto thought to be tooth specific, in the metabolically active ductal epithelial cells of human salivary glands. Furthermore, our report indicated that MMP20 co-expressed and potentially interacts with dentin sialophosphoprotein (DSPP), a member of the small integrin-binding ligand N-linked glycoproteins (SIBLINGs). Our earlier reports have shown the co-expression of three MMPs, MMP2, MMP3, and MMP9, with specific members of the SIBLING family: bone sialoprotein, osteopontin, and dentin matrix protein 1, respectively...
October 2016: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/27666429/localization-of-minodronate-in-mouse-femora-through-isotope-microscopy
#16
Hiromi Hongo, Muneteru Sasaki, Sachio Kobayashi, Tomoka Hasegawa, Tomomaya Yamamoto, Kanako Tsuboi, Erika Tsuchiya, Tomoya Nagai, Naznin Khadiza, Miki Abe, Ai Kudo, Kimimitsu Oda, Paulo Henrique Luiz de Freitas, Minqi Li, Hisayoshi Yurimoto, Norio Amizuka
Minodronate is highlighted for its marked and sustained effects on osteoporotic bones. To determine the duration of minodronate's effects, we have assessed the localization of the drug in mouse bones through isotope microscopy, after labeling it with a stable nitrogen isotope ([(15)N]-minodronate). In addition, minodronate-treated bones were assessed by histochemistry and transmission electron microscopy (TEM). Eight-week-old male ICR mice received [(15)N]-minodronate (1 mg/kg) intravenously and were sacrificed after 3 hr, 24 hr, 1 week, and 1 month...
October 2016: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/27591097/investigation-of-pd-l1-biomarker-testing-methods-for-pd-1-axis-inhibition-in-non-squamous-non-small-cell-lung-cancer
#17
Brandon S Sheffield, Regan Fulton, Steve E Kalloger, Katy Milne, Georgia Geller, Martin Jones, Celine Jacquemont, Susanna Zachara, Eric Zhao, Erin Pleasance, Janessa Laskin, Steven J M Jones, Marco A Marra, Stephen Yip, Brad H Nelson, Allen M Gown, Cheryl Ho, Diana N Ionescu
Inhibitors of the programmed cell death 1 (PD-1) signaling axis have recently demonstrated efficacy and are rapidly being incorporated into the treatment of non-small cell lung cancers (NSCLCs). Despite clear benefits to certain patients, the association of these responses with a predictive biomarker remains uncertain. Several different biomarkers have been proposed, with differing results and conclusions. This study compares multiple methods of biomarker testing for treatment of NSCLCs with PD1-axis inhibitors...
October 2016: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/27555495/comments-on-methods-to-suppress-endogenous-%C3%AE-galactosidase-activity-in-mouse-tissues-expressing-the-lacz-reporter-gene
#18
Claudia Merkwitz, Orest Blaschuk, Angela Schulz, Albert Markus Ricken
The Escherichia coli LacZ gene (encoding β-galactosidase) is a widely used reporter for gene regulation analysis in transgenic mice. Determination of β-galactosidase activity is classically performed using 5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside/ferri-/ferrocyanide (X-Gal/FeCN) histochemistry. Uncertainty about the origin of the β-galactosidase signal is encountered in tissues containing high levels of endogenous β-galactosidase. Here, we show that reliable results can nevertheless be obtained in these tissues by performing the histochemical reaction under slightly basic pH conditions (pH 8-9)...
October 2016: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/27578327/clinical-significance-of-tipe2-protein-upregulation-in-non-hodgkin-s-lymphoma
#19
Chunyan Hao, Na Zhang, Minghong Geng, Qing Ren, Yan Li, Yan Wang, Youhai H Chen, Suxia Liu
Non-Hodgkin's lymphoma (NHL), which includes diffuse large B-cell lymphoma (DLBCL) and peripheral T-cell lymphoma, is a refractory malignant tumor originated from the lymphatic system. TNFAIP8L2 (TIPE2 or tumor necrosis-alpha-induced protein-8 like 2) is a negative regulator for inflammation and an inhibitor for carcinogenesis. However, whether TIPE2 plays a role in lymphomagenesis is unknown. In this study, we determined TIPE2 expression in NHL by immunohistochemistry and investigated its clinicopathological significance in DLBCL...
September 2016: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/27578326/the-use-of-cytochrome-c-oxidase-enzyme-activity-and-immunohistochemistry-in-defining-mitochondrial-injury-in-kidney-disease
#20
Zsuzsanna K Zsengellér, Seymour Rosen
The renal biopsy is a dynamic way of looking at renal disease, and tubular elements are an important part of this analysis. The mitochondria in 20 renal biopsies were examined by immunohistochemical (electron transport chain enzyme: cytochrome C oxidase IV [COX IV]) and enzyme histochemical methods (COX), both by light and electron microscopy. The distal convoluted tubules and thick ascending limbs showed the greatest intensity in the COX immunostains and enzyme activity in controls. The degree of mitochondrial COX protein and enzyme activity diminished as the tubules became atrophic...
September 2016: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
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