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Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society

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https://www.readbyqxmd.com/read/28170292/heparanase-mediates-intestinal-inflammation-and-injury-in-a-mouse-model-of-sepsis
#1
Song Chen, Ying He, Ziwei Hu, Siyu Lu, Xiaohan Yin, Xiaochun Ma, Chuanzhu Lv, Guiyun Jin
Heparanase, a heparan sulfate (HS)-specific endoglycosidase, plays an important role in inflammation and mediates acute pulmonary and renal injuries during sepsis. To explore its role in septic intestinal injury, a non-anticoagulant heparanase inhibitor, N-desulfated/re- N-acetylated heparin (NAH), was administrated to a mouse sepsis model induced by cecal ligation and puncture (CLP). Immunohistochemical staining revealed massive shedding of HS from the intestinal mucosal surfaces after CLP, and effective inhibition of heparanase by NAH was confirmed by markedly reduced HS shedding...
January 1, 2017: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/28151698/immunocytochemical-profiling-of-cultured-mouse-primary-retinal-cells
#2
Marina C Zalis, Sebastian Johansson, Ulrica Englund-Johansson
Primary retinal cell cultures and immunocytochemistry are important experimental platforms in ophthalmic research. Translation of retinal cells from their native environment to the in vitro milieu leads to cellular stress, jeopardizing their in vivo phenotype features. Moreover, the specificity and stability of many retinal immunochemical markers are poorly evaluated in retinal cell cultures. Hence, we here evaluated the expression profile of 17 retinal markers, that is, recoverin, rhodopsin, arrestin, Chx10, PKC, DCX, CRALBP, GS, vimentin, TPRV4, RBPMS, Brn3a, β-tubulin III, NeuN, MAP2, GFAP, and synaptophysin...
January 1, 2017: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/28146365/advantages-and-limitations-of-salmon-gal-tetrazolium-salt-histochemistry-for-the-detection-of-lacz-reporter-gene-activity-in-murine-epithelial-tissue
#3
Claudia Merkwitz, Orest Blaschuk, Jana Winkler, Angela Schulz, Simone Prömel, Albert Markus Ricken
The Escherichia coli LacZ gene is a widely used reporter for gene regulation studies in transgenic mice. It encodes bacterial β-galactosidase (Bact β-Gal), which causes insoluble precipitates when exposed to chromogenic homologues of galactose. We and others have recently reported that Bact β-Gal detection with Salmon-Gal (S-Gal) in combination with nitro blue tetrazolium chloride (NBT) is very sensitive and not prone to interference by acidic endogenous β-galactosidases. Unfortunately, as we show here, the method appears to be inadequate for evaluation of Bact β-Gal expression in keratinized epithelial appendages but not in other keratinized epithelia...
January 1, 2017: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/28122194/histochemical-examination-on-periodontal-tissues-of-klotho-deficient-mice-fed-with-phosphate-insufficient-diet
#4
Kumiko Hikone, Tomoka Hasegawa, Erika Tsuchiya, Hiromi Hongo, Muneteru Sasaki, Tomomaya Yamamoto, Ai Kudo, Kimimitsu Oda, Mai Haraguchi, Paulo Henrique Luiz de Freitas, Minqi Li, Junichiro Iida, Norio Amizuka
To elucidate which of elevated serum concentration of inorganic phosphate (Pi) or disrupted signaling linked to αklotho/fibroblast growth factor 23 (FGF23) is a predominant regulator for senescence-related degeneration seen in αKlotho-deficient mice, we have examined histological alteration of the periodontal tissues in the mandibular interalveolar septum of αKlotho-deficient mice fed with Pi-insufficient diet. We prepared six groups of mice: wild-type, kl/kl, and αKlotho(-/-) mice with normal diet or low-Pi diet...
January 1, 2017: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/28112549/survival-related-autophagic-activity-versus-procalcific-death-in-cultured-aortic-valve-interstitial-cells-treated-with-critical-normophosphatemic-like-phosphate-concentrations
#5
Antonella Bonetti, Alberto Della Mora, Magali Contin, Giorgia Gregoraci, Franco Tubaro, Maurizio Marchini, Fulvia Ortolani
Valve dystrophic calcification is a common disorder affecting normophosphatemic subjects. Here, cultured aortic valve interstitial cells (AVICs) were treated 3 to 28 days with phosphate (Pi) concentrations spanning the normal range in humans (0.8, 1.3, and 2.0 mM) alone or supplemented with proinflammatory stimuli to assess possible priming of dystrophic-like calcification. Compared with controls, spectrophotometric analyses revealed marked increases in calcium amounts and alkaline phosphatase activity for 2...
March 2017: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/28080173/evaluation-of-long-term-cryostorage-of-brain-tissue-sections-for-quantitative-histochemistry
#6
Larissa I Estrada, Amy A Robinson, Ana C Amaral, Eustathia L Giannaris, Nadine C Heyworth, Farzad Mortazavi, Laura B Ngwenya, Debra E Roberts, Howard J Cabral, Ronald J Killiany, Douglas L Rosene
Storage of tissue sections for long periods allows multiple samples, acquired over months or years, to be processed together, in the same reagents, for quantitative histochemical studies. Protocols for freezer storage of free-floating frozen sections using sucrose with different additives have been reported and assert that storage has no effect on histochemistry, but no quantitative support has been provided. The present study analyzed the efficacy of long-term storage of brain tissue sections at -80C in buffered 15% glycerol...
March 2017: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/28056182/noise-stress-induces-an-epidermal-growth-factor-receptor-xeroderma-pigmentosum-a-response-in-the-auditory-nerve
#7
O'neil W Guthrie
In response to toxic stressors, cancer cells defend themselves by mobilizing one or more epidermal growth factor receptor (EGFR) cascades that employ xeroderma pigmentosum-A (XPA) to repair damaged genes. Recent experiments discovered that neurons within the auditory nerve exhibit basal levels of EGFR+XPA co-expression. This finding implied that auditory neurons in particular or neurons in general have the capacity to mobilize an EGFR+XPA defense. Therefore, the current study tested the hypothesis that noise stress would alter the expression pattern of EGFR/XPA within the auditory nerve...
March 2017: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/27881474/cancer-secretome-may-influence-bsp-and-dsp-expression-in-human-salivary-gland-cells
#8
Samantha Lynn Hamilton, Blake Ferando, Asha Sarah Eapen, Jennifer Chian Yu, Anita Rose Joy
One of the biggest challenges in managing head and neck cancers, especially salivary gland cancers, is the identification of secreted biomarkers of the disease that can be evaluated noninvasively. A relevant source of enriched tumor markers could potentially be found in the tumor secretome. Although numerous studies have evaluated secretomes from various cancers, the influence of the cancer secretome derived from salivary gland cancers on the behavior of normal cells has not yet been elucidated. Our data indicate that secretome derived from salivary gland cancer cells can influence the expression of two potential biomarkers of oral cancer-namely, bone sialoprotein (BSP) and dentin sialoprotein (DSP)-in normal salivary gland cells...
March 2017: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/28117631/intracellular-position-of-centrioles-and-the-direction-of-homeostatic-epithelial-cell-movements-in-the-mouse-cornea
#9
Erika Silverman, Jin Zhao, John C Merriam, Takayuki Nagasaki
Corneal epithelial cells exhibit continuous centripetal movements at a rate of about 30 µm per day, but neither the driving force nor the mechanism that determines the direction of movements is known. To facilitate the investigation of homeostatic cell movement, we examined if the intracellular position of a centriole can be used as a directional marker of epithelial cell movements in the mouse cornea. A direction of cell movements was estimated in fixed specimens from a pattern of underlying subepithelial nerve fibers...
February 2017: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/28117630/preventing-false-negatives-for-histochemical-detection-of-phenolics-and-lignins-in-peg-embedded-plant-tissues
#10
Bruno G Ferreira, Renan Falcioni, Lubia M Guedes, Sofia C Avritzer, Werner C Antunes, Luiz A Souza, Rosy M S Isaias
Polyethylene glycol (PEG) is a low-cost and advantageous embedding medium, which maintains the majority of cell contents unaltered during the embedding process. Some hard or complex plant materials are better embedded in PEG than in other usual embedding media. However, the histochemical tests for phenolics and lignins in PEG-embedded plant tissues commonly result in false negatives. We hypothesize that these false negatives should be prevented by the use of distinct fixatives, which should avoid the bonds between PEG and phenols...
February 2017: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/27856824/all-trans-retinoic-acid-augments-the-histopathological-outcome-of-neuroinflammation-and-neurodegeneration-in-lupus-prone-mrl-lpr-mice
#11
Michelle H Theus, Joshua B Sparks, Xiaofeng Liao, Jingjing Ren, Xin M Luo
Recently, we demonstrated that treatment with all- trans-retinoic acid (tRA) induced a paradoxical effect on immune activation during the development of autoimmune lupus. Here, we further describe its negative effects on mediating neuroinflammation and neurodegeneration. Female MRL/lpr mice were orally administered tRA or VARA (retinol mixed with 10% tRA) from 6 to 14 weeks of age. Both treatments had a significant effect on brain weight, which correlated with histopathological evidence of focal astrogliosis, meningitis, and ventriculitis...
February 2017: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/27789731/evaluation-of-akt-and-rictor-expression-levels-in-astrocytomas-of-all-grades
#12
Arthur William Alvarenga, Luis Eduardo Machado, Bruna Roz Rodrigues, Fernanda Cristina Sulla Lupinacci, Paulo Sanemastu, Eduardo Matta, Martín Roffé, Luís Fernando Bleggi Torres, Isabela Werneck da Cunha, Vilma Regina Martins, Glaucia Noeli Maroso Hajj
The mammalian target of rapamycin (mTOR) binds to several protein partners and forms two complexes, termed mTOR complexes 1 and 2 (mTORC1/2), that differ in components, substrates, and regulation. mTORC2 contains the protein Rapamycin-insensitive companion of mTOR (RICTOR); phosphorylates kinases of the AGC family, such as Akt; and controls the cytoskeleton. Even though the regulation of mTORC2 activity remains poorly understood, the hyperactivation of this signaling pathway has been shown to contribute to the oncogenic properties of gliomas in experimental models...
February 2017: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/28026654/l1cam-in-the-early-enteric-and-urogenital-system
#13
Elisabeth Judith Pechriggl, Nicole Concin, Michael J Blumer, Mario Bitsche, Marit Zwierzina, Jozsef Dudas, Katarzyna Koziel, Peter Altevogt, Alain-Gustave Zeimet, Helga Fritsch
L1 cell adhesion molecule (L1CAM) is a transmembrane molecule belonging to the L1 protein family. It has shown to be a key player in axonal guidance in the course of neuronal development. Furthermore, L1CAM is also crucial for the establishment of the enteric and urogenital organs and is aberrantly expressed in cancer originating in these organs. Carcinogenesis and embryogenesis follow a lot of similar molecular pathways, but unfortunately, comprehensive data on L1CAM expression and localization in human developing organs are lacking so far...
January 2017: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/27879410/triple-staining-including-foxa2-identifies-stem-cell-lineages-undergoing-hepatic-and-biliary-differentiation-in-cirrhotic-human-liver
#14
Charles E Rogler, Remon Bebawee, Joe Matarlo, Joseph Locker, Nicole Pattamanuch, Sanjeev Gupta, Leslie E Rogler
Recent investigations have reported many markers associated with human liver stem/progenitor cells, "oval cells," and identified "niches" in diseased livers where stem cells occur. However, there has remained a need to identify entire lineages of stem cells as they differentiate into bile ducts or hepatocytes. We have used combined immunohistochemical staining for a marker of hepatic commitment and specification (FOXA2 [Forkhead box A2]), hepatocyte maturation (Albumin and HepPar1), and features of bile ducts (CK19 [cytokeratin 19]) to identify lineages of stem cells differentiating toward the hepatocytic or bile ductular compartments of end-stage cirrhotic human liver...
January 2017: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/27872404/unique-cellular-lineage-composition-of-the-first-gland-of-the-mouse-gastric-corpus
#15
Andrew O'Neil, Christine P Petersen, Eunyoung Choi, Amy C Engevik, James R Goldenring
The glandular stomach has two major zones: the acid secreting corpus and the gastrin cell-containing antrum. Nevertheless, a single gland lies at the transition between the forestomach and corpus in the mouse stomach. We have sought to define the lineages that make up this gland unit at the squamocolumnar junction. The first gland in mice showed a notable absence of characteristic corpus lineages, including parietal cells and chief cells. In contrast, the gland showed strong staining of Griffonia simplicifolia-II (GSII)-lectin-positive mucous cells at the bases of glands, which were also positive for CD44 variant 9 and Clusterin...
January 2017: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/27798289/antigen-masking-during-fixation-and-embedding-dissected
#16
Carla Rossana Scalia, Giovanna Boi, Maddalena Maria Bolognesi, Lorella Riva, Marco Manzoni, Linde DeSmedt, Francesca Maria Bosisio, Susanna Ronchi, Biagio Eugenio Leone, Giorgio Cattoretti
Antigen masking in routinely processed tissue is a poorly understood process caused by multiple factors. We sought to dissect the effect on antigenicity of each step of processing by using frozen sections as proxies of the whole tissue. An equivalent extent of antigen masking occurs across variable fixation times at room temperature. Most antigens benefit from longer fixation times (>24 hr) for optimal detection after antigen retrieval (AR; for example, Ki-67, bcl-2, ER). The transfer to a graded alcohol series results in an enhanced staining effect, reproduced by treating the sections with detergents, possibly because of a better access of the polymeric immunohistochemical detection system to tissue structures...
January 2017: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/27837159/variability-in-platelet-derived-growth-factor-receptor-alpha-antibody-specificity-may-impact-clinical-utility-of-immunohistochemistry-assays
#17
Timothy R Holzer, Leslie O'Neill Reising, Kelly M Credille, Andrew E Schade, Gerard J Oakley
Aberrant regulation of the receptor tyrosine kinase platelet-derived growth factor alpha (PDGFRα) is implicated in several types of cancer. Inhibition of the PDGFRα pathway may be a beneficial therapy, and detection of PDGFRα in tumor biopsies may lead to insights about which patients respond to therapy. Exploratory or clinical biomarker use of PDGFRα IHC has been frequently reported, often with polyclonal antibody sc-338. An sc-338-based assay was systematically compared with anti-PDGFRα rabbit monoclonal antibody D13C6 using immunoblot profiling and IHC in formalin-fixed and paraffin-embedded human tumor cell lines...
December 2016: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/27798288/cell-line-macroarray-an-alternative-high-throughput-platform-to-analyze-hipsc-lines
#18
Alberto La Spada, Simona Baronchelli, Linda Ottoboni, Francesca Ruffini, Gianvito Martino, Nunzia Convertino, Aikaterini Ntai, Tobias Steiner, Ida Biunno, Andrea De Blasio
In the past decade, tissue microarray (TMA) technology has evolved as an innovative tool for high-throughput proteomics analysis and mainly for biomarker validation. Similarly, enormous amount of data can be obtained from the cell line macroarray (CLMA) technology, which developed from the TMA using formalin-fixed, paraffin-embedded cell pellets. Here, we applied CLMA technology in stem cell research and in particular to identify bona fide neogenerated human induced pluripotent stem cell (hiPSC) clones suitable for down the line differentiation...
December 2016: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/27798287/altered-t-cell-balance-in-lymphoid-organs-of-a-mouse-model-of-colorectal-cancer
#19
Scott M Tanner, Joseph G Daft, Stephanie A Hill, Colin A Martin, Robin G Lorenz
The adenomatous polyposis coli (APC) gene is a known tumor suppressor gene, and mice with mutations in Apc (Apc(Min/+)) spontaneously form multiple intestinal neoplasms. In this model of human colorectal cancer (CRC), it has been reported that CD4(+) T-cell-derived interleukin 17 (IL-17) promotes intestinal tumor development, but it is not known if the Apc mutation actually directly alters T-cell function and subsequently tumor immunosurveillance. To investigate the Apc(Min/+) mutation on T-cell function, flow cytometric, histochemical, and immunofluorescent studies on both wild-type (Apc(+/+)) and Apc(Min/+) mice were performed...
December 2016: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/27798286/megalin-is-predominantly-observed-in-vesicular-structures-in-first-and-third-trimester-cytotrophoblasts-of-the-human-placenta
#20
Tina Storm, Erik I Christensen, Julie Nelly Christensen, Tine Kjaergaard, Niels Uldbjerg, Agnete Larsen, Bent Honoré, Mette Madsen
The membrane receptor megalin is crucial for normal fetal development. Besides its expression in the developing fetus, megalin is also expressed in the human placenta. Similar to its established function in the kidney proximal tubules, placental megalin has been proposed to mediate uptake of vital nutrients. However, details of megalin expression, subcellular localization, and function in the human placenta remain to be established. By immunohistochemical analyses of first trimester and term human placenta, we showed that megalin is predominantly expressed in cytotrophoblasts, the highly proliferative cells in placenta...
December 2016: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
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