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Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society

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https://www.readbyqxmd.com/read/28530841/the-increased-expression-of-toll-like-receptor-4-in-renal-and-skin-lesions-in-lupus-erythematosus
#1
Nesrine Elloumi, Raouia Fakhfakh, Lobna Ayadi, Khadija Sellami, Olfa Abida, Mariem Ben Jmaa, Tahya Sellami, Khawla Kammoun, Hatem Masmoudi
Toll-like receptor 4 (TLR-4), a bacterial lipopolysaccharide sensor, is an innate immunity essential modulator. It is expressed on both immune and non-immune cells and may contribute to the cutaneous and renal manifestations during lupus erythematosus (LE). Our purpose is to evaluate TLR-4 expression and analyzing its role in lupus nephritis (LN) and chronic cutaneous lupus erythematosus (CLE) pathogenesis. TLR-4 immunohistochemical staining was performed on 30 LN renal biopsies compared with 11 healthy renal tissues and 30 skin biopsies from CLE patients compared with 15 normal individuals...
May 1, 2017: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/28415912/a-method-for-the-immunohistochemical-identification-and-localization-of-osterix-in-periosteum-wrapped-constructs-for-tissue-engineering-of-bone
#2
Phillip McClellan, Robin Jacquet, Qing Yu, William J Landis
A novel immunohistochemistry (IHC) approach has been developed to label and localize osterix, a bone-specific transcription factor, within formalin-fixed, paraffin-embedded, tissue-engineered constructs uniquely containing synthetic polymers and human periosteal tissue. Generally, such specimens consisting in part of polymeric materials and mineral are particularly difficult for IHC identification of proteins. Samples here were fabricated from human periosteum, electrospun poly-l-lactic acid (PLLA) nanofibers, and polycaprolactone/poly-l-lactic acid (PCL/PLLA, 75/25) scaffolds and harvested following 10 weeks of implantation in athymic mice...
April 1, 2017: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/28402755/distribution-of-fcrn-across-species-and-tissues
#3
Sari Latvala, Bjoern Jacobsen, Michael B Otteneder, Annika Herrmann, Sven Kronenberg
The neonatal Fc receptor (FcRn) is a major histocompatibility complex class I type molecule that binds to, transports, and recycles immunoglobulin G (IgG) and albumin, thereby protecting them from lysosomal degradation. Therefore, besides the knowledge of FcRn affinity, FcRn protein expression is critical in understanding the pharmacokinetic behavior of Fc-containing biotherapeutics such as monoclonal antibodies. The goal of this investigation was to achieve for the first time a comparative assessment of FcRn distribution across a variety of tissues and species...
April 1, 2017: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/28402752/the-microrna-mir-210-is-expressed-by-cancer-cells-but-also-by-the-tumor-microenvironment-in-triple-negative-breast-cancer
#4
Isabelle Bar, Ahmad Merhi, Fadi Abdel-Sater, Abduelhakem Ben Addi, Sara Sollennita, Jean-Luc Canon, Paul Delrée
The triple-negative breast cancer (TNBC) subtype occurs in about 15% of breast cancer and is an aggressive subtype of breast cancer with poor outcome. Furthermore, treatment of patients with TNBC is more challenging due to the heterogeneity of the disease and the absence of well-defined molecular targets. Microribonucleic acid (RNA) represents a new class of biomarkers that are frequently dysregulated in cancer. It has been described that the microRNA miR-210 is highly expressed in TNBC, and its overexpression had been linked to poor prognosis...
April 1, 2017: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/28388264/different-patterns-of-cartilage-mineralization-analyzed-by-comparison-of-human-porcine-and-bovine-laryngeal-cartilages
#5
Horst Claassen, Martin Schicht, Bernd Fleiner, Ralf Hillmann, Sebastian Hoogeboom, Bernhard Tillmann, Friedrich Paulsen
Laryngeal cartilages undergo a slow ossification process during aging, making them an excellent model for studying cartilage mineralization and ossification processes. Pig laryngeal cartilages are similar to their human counterparts in shape and size, also undergo mineralization, facilitating the study of cartilage mineralization. We investigated the processes of cartilage mineralization and ossification and compared these with the known processes in growth plates. Thyroid cartilages from glutaraldehyde-perfused male minipigs and from domestic pigs were used for X-ray, light microscopic, and transmission electron microscopic analyses...
April 1, 2017: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/28375676/tyrosine-phosphorylation-of-an-actin-binding-protein-girdin-specifically-marks-tuft-cells-in-human-and-mouse-gut
#6
Daisuke Kuga, Kaori Ushida, Shinji Mii, Atsushi Enomoto, Naoya Asai, Masato Nagino, Masahide Takahashi, Masato Asai
Tuft cells (TCs) are minor components of gastrointestinal epithelia, characterized by apical tufts and spool-shaped somas. The lack of reliable TC-markers has hindered the elucidation of its role. We developed site-specific and phosphorylation-status-specific antibodies against Girdin at tyrosine-1798 (pY1798) and found pY1798 immunostaining of mouse jejunum clearly depicted epithelial cells closely resembling TCs. This study aimed to validate pY1798 as a TC-marker. Double-fluorescence staining of intestines was performed with pY1798 and known TC-markers, for example, hematopoietic-prostaglandin-D-synthase (HPGDS), or doublecortin-like kinase 1 (DCLK1)...
April 1, 2017: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/28438094/association-of-atf4-expression-with-tissue-hypoxia-and-m2-macrophage-infiltration-in-infantile-hemangioma
#7
Hou-Fu Xia, Jun-Yi Zhu, Jing-Nan Wang, Jian-Gang Ren, Yu Cai, Feng-Qin Wang, Wei Zhang, Gang Chen, Yi-Fang Zhao, Ji-Hong Zhao
Accumulating studies have revealed the hypoxic condition and its crucial role in the distinctive progression of infantile hemangioma (IH), the most common benign tumor in infancy. Activating transcription factor 4 (ATF4), an important gene mediating cellular adaptation to various stress signals, could confer a survival advantage for tumor cells under hypoxia and regulate tumor progression. However, the potential role of ATF4 in IH was still unknown. In this study, the expression of hypoxia inducible factor (HIF)-1α, ATF4, and macrophage colony-stimulating factor (M-CSF) in 27 specimens of IH was measured by immunochemistry and double-labeling immunofluorescence, followed by the Spearman rank correlation test...
May 2017: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/28438093/tyramide-signal-amplification-permits-immunohistochemical-analyses-of-androgen-receptors-in-the-rat-prefrontal-cortex
#8
Katelyn L Low, Chunqi Ma, Kiran K Soma
Research on neural androgen receptors (ARs) has traditionally focused on brain regions that regulate reproductive and aggressive behaviors, such as the hypothalamus and amygdala. Although many cells in the prefrontal cortex (PFC) also express ARs, the number of ARs per cell appears to be much lower, and thus, AR immunostaining is often hard to detect and quantify in the PFC. Here, we demonstrate that biotin tyramide signal amplification (TSA) dramatically increases AR immunoreactivity in the rat brain, including critical regions of the PFC such as the medial PFC (mPFC) and orbitofrontal cortex (OFC)...
May 2017: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/28438092/characterization-by-lectin-histochemistry-of-two-subpopulations-of-parietal-cells-in-the-rat-gastric-glands
#9
Laura Gómez-Santos, Edurne Alonso, Lucio Díaz-Flores, Juan F Madrid, Francisco J Sáez
Parietal cells undergo a differentiation process while they move from the isthmus toward the pits and the base region of the gastric gland. The aim of this work was to analyze the rat gastric glands by lectin histochemistry to show the glycans expressed by upper (young) and lower (old) parietal cells. We used lectins recognizing the most frequent sugar moieties in mammals. Each lectin was assayed alone and in combination with several deglycosylation pretreatments: (1) β-elimination, which removes O-linked oligosaccharides; (2) incubation with Peptide-N-glycosidase F, to remove N-linked glycans; (3) acid hydrolysis, which removes terminal sialic acid moieties; (4) methylation-saponification, to remove sulfate groups from sugar residues; and (5) glucose oxidase, a technique carried out with the lectin concanavalin A to convert glucose into gluconic acid...
May 2017: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/28438091/analytic-response-curves-of-clinical-breast-cancer-ihc-tests
#10
Kodela Vani, Seshi R Sompuram, Anika K Schaedle, Anuradha Balasubramanian, Steven A Bogen
An important limitation in the field of immunohistochemistry (IHC) is the inability to correlate stain intensity with specific analyte concentrations. Clinical immunohistochemical tests are not described in terms of analytic response curves, namely, the analyte concentrations in a tissue sample at which an immunohistochemical stain (1) is first visible, (2) increases in proportion to the analyte concentration, and (3) ultimately approaches a maximum color intensity. Using a new immunostaining tool ( IHControls), we measured the analytic response curves of the major clinical immunohistochemical tests for human epidermal growth factor receptor type II (HER-2), estrogen receptor (ER), and progesterone receptor (PR)...
May 2017: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/28347267/buffered-ethanol-fixative
#11
John A Kiernan
No abstract text is available yet for this article.
April 2017: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/28347266/formulation-and-ph-of-the-buffered-ethanol-fixative-be70
#12
Stephen M Hewitt
No abstract text is available yet for this article.
April 2017: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/28347265/response-to-inquiry-concerning-which-polyclonal-goat-antibody-for-detection-of-flag-tag-in-formalin-fixed-and-paraffin-embedded-specimens
#13
Ronald E Ferrando
No abstract text is available yet for this article.
April 2017: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/28347264/inquiry-concerning-which-polyclonal-goat-antibody-for-detection-of-flag-tag-in-formalin-fixed-and-paraffin-embedded-specimens
#14
Enrico Radaelli, Matteo Riva
No abstract text is available yet for this article.
April 2017: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/28347263/editor-s-note
#15
Stephen M Hewitt
No abstract text is available yet for this article.
April 2017: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/28170292/heparanase-mediates-intestinal-inflammation-and-injury-in-a-mouse-model-of-sepsis
#16
Song Chen, Ying He, Ziwei Hu, Siyu Lu, Xiaohan Yin, Xiaochun Ma, Chuanzhu Lv, Guiyun Jin
Heparanase, a heparan sulfate (HS)-specific endoglycosidase, plays an important role in inflammation and mediates acute pulmonary and renal injuries during sepsis. To explore its role in septic intestinal injury, a non-anticoagulant heparanase inhibitor, N-desulfated/re- N-acetylated heparin (NAH), was administrated to a mouse sepsis model induced by cecal ligation and puncture (CLP). Immunohistochemical staining revealed massive shedding of HS from the intestinal mucosal surfaces after CLP, and effective inhibition of heparanase by NAH was confirmed by markedly reduced HS shedding...
April 2017: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/28151698/immunocytochemical-profiling-of-cultured-mouse-primary-retinal-cells
#17
Marina C Zalis, Sebastian Johansson, Ulrica Englund-Johansson
Primary retinal cell cultures and immunocytochemistry are important experimental platforms in ophthalmic research. Translation of retinal cells from their native environment to the in vitro milieu leads to cellular stress, jeopardizing their in vivo phenotype features. Moreover, the specificity and stability of many retinal immunochemical markers are poorly evaluated in retinal cell cultures. Hence, we here evaluated the expression profile of 17 retinal markers, that is, recoverin, rhodopsin, arrestin, Chx10, PKC, DCX, CRALBP, GS, vimentin, TPRV4, RBPMS, Brn3a, β-tubulin III, NeuN, MAP2, GFAP, and synaptophysin...
April 2017: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/28146365/advantages-and-limitations-of-salmon-gal-tetrazolium-salt-histochemistry-for-the-detection-of-lacz-reporter-gene-activity-in-murine-epithelial-tissue
#18
Claudia Merkwitz, Orest Blaschuk, Jana Winkler, Angela Schulz, Simone Prömel, Albert Markus Ricken
The Escherichia coli LacZ gene is a widely used reporter for gene regulation studies in transgenic mice. It encodes bacterial β-galactosidase (Bact β-Gal), which causes insoluble precipitates when exposed to chromogenic homologues of galactose. We and others have recently reported that Bact β-Gal detection with Salmon-Gal (S-Gal) in combination with nitro blue tetrazolium chloride (NBT) is very sensitive and not prone to interference by acidic endogenous β-galactosidases. Unfortunately, as we show here, the method appears to be inadequate for evaluation of Bact β-Gal expression in keratinized epithelial appendages but not in other keratinized epithelia...
April 2017: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/28122194/histochemical-examination-on-periodontal-tissues-of-klotho-deficient-mice-fed-with-phosphate-insufficient-diet
#19
Kumiko Hikone, Tomoka Hasegawa, Erika Tsuchiya, Hiromi Hongo, Muneteru Sasaki, Tomomaya Yamamoto, Ai Kudo, Kimimitsu Oda, Mai Haraguchi, Paulo Henrique Luiz de Freitas, Minqi Li, Junichiro Iida, Norio Amizuka
To elucidate which of elevated serum concentration of inorganic phosphate (Pi) or disrupted signaling linked to αklotho/fibroblast growth factor 23 (FGF23) is a predominant regulator for senescence-related degeneration seen in αKlotho-deficient mice, we have examined histological alteration of the periodontal tissues in the mandibular interalveolar septum of αKlotho-deficient mice fed with Pi-insufficient diet. We prepared six groups of mice: wild-type, kl/kl, and αKlotho(-/-) mice with normal diet or low-Pi diet...
April 2017: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/28112549/survival-related-autophagic-activity-versus-procalcific-death-in-cultured-aortic-valve-interstitial-cells-treated-with-critical-normophosphatemic-like-phosphate-concentrations
#20
Antonella Bonetti, Alberto Della Mora, Magali Contin, Giorgia Gregoraci, Franco Tubaro, Maurizio Marchini, Fulvia Ortolani
Valve dystrophic calcification is a common disorder affecting normophosphatemic subjects. Here, cultured aortic valve interstitial cells (AVICs) were treated 3 to 28 days with phosphate (Pi) concentrations spanning the normal range in humans (0.8, 1.3, and 2.0 mM) alone or supplemented with proinflammatory stimuli to assess possible priming of dystrophic-like calcification. Compared with controls, spectrophotometric analyses revealed marked increases in calcium amounts and alkaline phosphatase activity for 2...
March 2017: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
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