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Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society

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https://www.readbyqxmd.com/read/30431382/the-influence-of-tgf-%C3%AE-3-egf-and-bgn-on-sox9-and-runx2-expression-in-human-chondrogenic-progenitor-cells
#1
Jerome Nicolas Janssen, Sarah Batschkus, Stefan Schimmel, Christa Bode, Boris Schminke, Nicolai Miosge
Osteoarthritis (OA) is the most common chronic joint disease and leads to the degradation of the extracellular matrix by an imbalance between anabolic and catabolic processes. TGF-β3 (transforming growth factor beta-3) and epidermal growth factor (EGF) influence the osteochondrogenic potential of chondrocytes. In this study, we compared the expression of mediators and receptors in the TGF-β3 and EGF pathways, as well as biglycan (BGN), in healthy and diseased chondrocytes. Furthermore, we used chondrogenic progenitor cells (CPCs) for in vitro stimulation and knockdown experiments to elucidate the effects of TGF-β3 and EGF on the chondrogenic potential...
November 15, 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/30380361/subcellular-localization-of-prolyl-endopeptidase-during-the-first-wave-of-rat-spermatogenesis-and-in-rat-and-human-sperm
#2
Massimo Venditti, Sergio Minucci
Prolyl endopeptidase (PREP) is an enzyme which cleaves several peptide hormones and neuropeptides on the carboxyl side of proline residues and is involved in many biological processes, including cell proliferation and differentiation, glucose metabolism, learning, memory, and cognitive disorders. PREP has also been identified as a binding partner of tubulin, suggesting the involvement of endopeptidase in microtubule-associate processes, independent of its peptidase activity. Furthermore, several reports have implied PREP participation in both male and female reproduction-associated mechanism...
October 31, 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/30311820/histological-effects-of-the-combined-administration-of-eldecalcitol-and-a-parathyroid-hormone-in-the-metaphyseal-trabeculae-of-ovariectomized-rats
#3
Tomoka Hasegawa, Tomomaya Yamamoto, Sadaoki Sakai, Yukina Miyamoto, Hiromi Hongo, Zixuan Qiu, Miki Abe, Satoshi Takeda, Kimimitsu Oda, Paulo Henrique Luiz de Freitas, Minqi Li, Koichi Endo, Norio Amizuka
Intermittent administration of human parathyroid hormone (1-34) (hPTH(1-34)) promotes anabolic action in bone by stimulating bone remodeling, while eldecalcitol, an analog of active vitamin D3, suppresses osteoclastic bone resorption, and forms new bone by minimodeling. We have examined the biological effects of combined administration of eldecalcitol and hPTH(1-34) on 9-week-old Wistar rats that underwent an ovariectomy (OVX) or Sham operation. They were divided into a Sham group, OVX with vehicle (OVX group), OVX with 10 µg/kg/day of hPTH(1-34) (PTH group), OVX with 20 ng/kg/day of eldecalcitol (eldecalcitol group) or OVX with 10 μg/kg/day of hPTH(1-34), and 20 ng/kg/day of eldecalcitol (combined group) for 4 or 8 weeks...
October 12, 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/30303767/prevention-of-cell-growth-by-suppression-of-villin-expression-in-lithocholic-acid-stimulated-hepg2-cells
#4
Munetaka Ozeki, Wulamujiang Aini, Aya Miyagawa-Hayashino, Keiji Tamaki
Cholestasis is a condition wherein bile flow is interrupted and lithocholic acid is known to play a key role in causing severe liver injury. In this study, we performed in-depth analysis of the morphological changes in bile canaliculi and the biological role of villin in cholestasis using lithocholic acid-stimulated HepG2 human hepatocarcinoma cells. We confirmed disruption of the bile canaliculi in liver sections from a liver allograft patient with cholestasis. Lithocholic acid caused strong cytotoxicity in HepG2 cells, which was associated with abnormal morphology...
October 10, 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/30265185/protein-kinase-r-is-constitutively-expressed-in-the-human-pancreas
#5
Alexander Jonsson, Erik Yngve, Marie Karlsson, Sofie Ingvast, Oskar Skog, Olle Korsgren
Viral infection of the insulin-producing cells in the pancreas has been proposed in the etiology of type 1 diabetes. Protein kinase R (PKR) is a cytoplasmic protein activated through phosphorylation in response to cellular stress and particularly viral infection. As PKR expression in pancreatic beta-cells has been interpreted as a viral footprint, this cross-sectional study aimed at characterizing the PKR expression in nondiabetic human pancreases. PKR expression was evaluated in pancreas tissue from 16 nondiabetic organ donors, using immunohistochemistry, qPCR, and western blot...
September 28, 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/30216108/scc-s2-facilitates-tumor-proliferation-and-invasion-via-activating-wnt-signaling-and-depressing-hippo-signaling-in-colorectal-cancer-cells-and-predicts-poor-prognosis-of-patients
#6
Chuanjia Yang, Weixue Xu, Xiangzhen Meng, Siqi Zhou, Minglu Zhang, Dongxu Cui
SCC-S2 overexpression has been implicated in several human cancers, its correlation with prognosis and the mechanism how it reserved biological roles are still uncertain. The current study demonstrated that, in 142 archived colorectal carcinoma (CRC) tissue samples, SCC-S2 expression was significantly correlated with higher histological grade ( p=0.001), tumor invasion ( p=0.001), advanced Dukes staging ( p=0.002), positive regional lymph node metastasis ( p=0.024), and poor overall survival ( p<0.001). MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and Transwell assays showed that SCC-S2 significantly promoted the proliferation and invasion...
September 14, 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/30205019/effect-of-polarization-and-chronic-inflammation-on-macrophage-expression-of-heparan-sulfate-proteoglycans-and-biosynthesis-enzymes
#7
Maarten Swart, Linda Troeberg
Heparan sulfate (HS) proteoglycans on immune cells have the ability to bind to and regulate the bioactivity more than 400 bioactive protein ligands, including many chemokines, cytokines, and growth factors. This makes them important regulators of the phenotype and behavior of immune cells. Here we review how HS biosynthesis in macrophages is regulated during polarization and in chronic inflammatory diseases such as rheumatoid arthritis, atherosclerosis, asthma, chronic obstructive pulmonary disease and obesity, by analyzing published micro-array data and mechanistic studies in this area...
September 11, 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/30199656/refined-immunochemical-characterization-in-healthy-dog-skin-of-the-epidermal-cornification-proteins-filaggrin-and-corneodesmosin
#8
Didier Pin, Valérie Pendaries, Sokhna Keita Alassane, Carine Froment, Nicolas Amalric, Marie-Christine Cadiergues, Guy Serre, Marek Haftek, Emilie Vidémont, Michel Simon
Filaggrin (FLG) and corneodesmosin (CDSN) are two key proteins of the human epidermis. FLG loss-of-function mutations are the strongest genetic risk factors for human atopic dermatitis. Studies of the epidermal distribution of canine FLG and CDSN are limited. Our aim was to better characterize the distribution of FLG and CDSN in canine skin. Using immunohistochemistry on beagle skin, we screened a series of monoclonal antibodies (mAbs) specific for human FLG and CDSN. The cross-reactive mAbs were further used using immunoelectron microscopy and Western blotting...
September 10, 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/30138043/three-dimensional-cellular-raman-analysis-evidence-of-highly-ordered-lipids-within-cell-nuclei
#9
Bhagavathi Ramamurthy, Stanley Cohen, Mark Canales, Frederick D Coffman
Striking levels of spatial organization exist among and within interphase cell chromosomes, raising the possibility that other nuclear molecular components may also be organized in ways that facilitate nuclear function. To further examine molecular distributions and organization within cell nuclei, we utilized Raman spectroscopy to map distributions of molecular components, with a focus on cellular lipids. Although the vast majority of cellular lipids are associated with membranes, mapping the 2870/2850 cm-1 lipid peak ratios revealed that the most highly ordered lipids within interphase cells are found within cell nuclei...
August 23, 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/30052477/characterization-of-fgd5-expression-in-primary-breast-cancers-and-lymph-node-metastases
#10
Marit Valla, Patricia G Mjønes, Monica J Engstrøm, Borgny Ytterhus, Diana L Bordin, Barbara van Loon, Lars A Akslen, Lars J Vatten, Signe Opdahl, Anna M Bofin
Faciogenital dysplasia 5 ( FGD5) amplification drives tumor cell proliferation, and is present in 9.5% of breast cancers. We describe FGD5 expression, assess associations between FGD5 amplification and FGD5 expression, and assess FGD5 expression in relation to proliferation and prognosis. FGD5 immunohistochemistry was done on primary tumors ( n=829) and lymph node metastases ( n=231) from a cohort of Norwegian patients. We explored associations between FGD5 amplification, FGD5 expression, and proliferation, and analyzed the prognostic value of FGD5 expression by estimating cumulative risks of death and hazard ratios (HRs)...
November 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/29985723/characterization-of-epidermal-lipoxygenase-expression-in-normal-human-skin-and-tissue-engineered-skin-substitutes
#11
Carolyne Simard-Bisson, Lorraine Andrée Parent, Véronique J Moulin, Bernard Fruteau de Laclos
Lipoxygenases (LOXs) are enzymes likely to be involved in corneocyte lipid envelope formation and skin barrier function. In humans, mutations in epidermis-type lipoxygenase 3 ( eLOX-3) and 12R-lipoxygenase ( 12R-LOX) genes are associated with autosomal recessive congenital ichthyosis (ARCI), whereas deletion of these genes in mice causes epidermal defects. LOXs also represent a matter of interest in psoriasis as well as in cancer research. However, their expression as well as the exact role of these enzymes in normal human skin have not been fully described...
November 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/29873589/acute-and-chronic-iron-overloading-differentially-modulates-the-expression-of-cellular-iron-homeostatic-molecules-in-normal-rat-kidney
#12
Bassem Refaat, Abdelghany Hassan Abdelghany, Mohammad A BaSalamah, Mohamed El-Boshy, Jawwad Ahmad, Shakir Idris
Little is known about the renal responses to acute iron overloading. This study measured the renal tubular expression of transferrin receptor-1 (TfR1), cubilin/megalin receptors, hepcidin, ferroportin, and ferritin chains following subacute intoxication of 40 male Wistar rats with a single oral dose of ferrous iron (300 mg/kg). The animals were randomly subdivided into 4 equal subgroups at the time of necropsy (1, 2, 4, and 8 hr). The results were compared with the controls ( n=15) and with the chronic group ( n=15), which received iron for 4 weeks (75 mg/kg/day; 5 days/week)...
November 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/29775115/immunofluorescent-localization-of-non-myelinating-schwann-cells-and-their-interactions-with-immune-cells-in-mouse-thymus
#13
Dailun Hu, Philip K Nicholls, Changfu Yin, Khama Kelman, Qionglan Yuan, Wayne K Greene, Zhongli Shi, Bin Ma
The thymus is innervated by sympathetic/parasympathetic nerve fibers from the peripheral nervous system (PNS), suggesting a neural regulation of thymic function including T-cell development. Despite some published studies, data on the innervation and nerve-immune interaction inside the thymus remain limited. In the present study, we used immunofluorescent staining of glial fibrillary acidic protein (GFAP) coupled with confocal microscopy/three-dimensional (3D) reconstruction to reveal the distribution of non-myelinating Schwann cells (NMSC) and their interactions with immune cells inside mouse thymus...
November 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/29762076/the-degeneration-of-the-vestibular-efferent-neurons-after-intratympanic-gentamicin-administration
#14
Qianru Wu, Yibo Zhang, Chunfu Dai, Yu Kong, Lijun Pan
Intratympanic gentamicin (ITG) has been used to treat refractory Ménière's disease. Disequilibrium after ITG was still a challenge for some patients, and the underlying mechanism is poorly understood. Our previous study demonstrated that gentamicin distributed in the bilateral vestibular efferent neurons (VEN) after ITG; however, does it lead to VEN damage and cause further disequilibrium in patients following ITG? In this study, we observed severe damaged gentamicin-positive neurons of VEN and severe fractured myelin layer plates around neural fibers when viewed under transmission electron microscopy at day 3 after ITG...
November 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/30133331/runx3-epigenetic-inactivation-is-associated-with-estrogen-receptor-positive-breast-cancer
#15
Hui Liu, Zhantao Yan, Qianqian Yin, Kai Cao, Yu Wei, Jaime Rodriguez-Canales, Dongshen Ma, Yongping Wu
The role of Runt-related transcription factor 3 ( RUNX3) gene in breast cancer remains not fully understood. We studied the correlation between RUNX3 gene promoter methylation and estrogen receptor (ER) expression status in breast cancer. Three breast cancer cell lines and 113 formalin-fixed, paraffin-embedded breast cancer tissue samples were analyzed for RUNX3 expression. Methylation-specific polymerase chain reaction was used to analyze RUNX3 methylation on the samples. Migration and invasion ability were evaluated in MCF7 cell line (RUNX3 methylated) treated with methylation inhibitor 5-Aza-2'-deoxycytidine (5-Aza-CdR) to study the effect of RUNX3 methylation status...
October 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/30106598/4-6-diamidino-2-phenylindole-distinctly-labels-tau-deposits
#16
Chengyu Li, Tetsuya Takahashi, Tejashwi Shrestha, Eiji Kinoshita, Tomoyasu Matsubara, Masayasu Matsumoto, Hirofumi Maruyama
Tau deposits have distinct biochemical characteristics and vary morphologically based on identification with tau antibodies and several chemical dyes. Here, we report 4',6-diamidino-2-phenylindole (DAPI)-positivity of tau deposits. Furthermore, we investigated the cause for this positivity. DAPI was positive in 3R/4R (3-repeat/4-repeat) tau deposits in Alzheimer's disease, myotonic dystrophy, and neurodegeneration with brain iron accumulation, and in 4R tau deposits in corticobasal degeneration, but negative in 4R tau deposits in frontotemporal dementia with parkinsonism-17 and progressive supranuclear palsy...
October 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/29782809/the-effects-of-normal-aging-on-regional-accumulation-of-hyaluronan-and-chondroitin-sulfate-proteoglycans-in-the-mouse-brain
#17
May J Reed, Mamatha Damodarasamy, Jasmine L Pathan, Michelle A Erickson, William A Banks, Robert B Vernon
The brain changes in volume and composition with normal aging. Cellular components of the brain are supported by an extracellular matrix (ECM) comprised largely of hyaluronan (HA) and HA-associated members of the lectican family of chondroitin sulfate proteoglycans (CSPGs). We examined regional differences in microvascular density, neuronal and glial markers, and accumulation of HA and CSPGs in mouse brains during normal aging. The cortex, hippocampus, dentate gyrus, and cerebellum of young (4 months), middle-aged (14 months), and aged (24-26 months) brains were analyzed...
October 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/29775122/nitration-of-mnsod-in-the-carotid-body-and-adrenal-gland-induced-by-chronic-intermittent-hypoxia
#18
Esteban A Moya, Paulina Arias, Rodrigo Iturriaga
Chronic intermittent hypoxia (CIH), main feature of obstructive sleep apnea, produces nitro-oxidative stress, which contributes to potentiate carotid body (CB) chemosensory discharges and sympathetic-adrenal-axis activity, leading to hypertension. The MnSOD enzymatic activity, a key enzyme on oxidative stress control, is reduced by superoxide-induced nitration. However, the effects of CIH-induced nitration on MnSOD enzymatic activity in the CB and adrenal gland are not known. We studied the effects of CIH on MnSOD protein and immunoreactive (MnSOD-ir) levels in the CB, adrenal gland and superior cervical ganglion (SCG), and on 3-nitrotyrosine (3-NT-ir), CuZnSOD (CuZnSOD-ir), MnSOD nitration, and its enzymatic activity in the CB and adrenal gland from male Sprague-Dawley rats exposed to CIH for 7 days...
October 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/29741434/effects-of-oxaliplatin-treatment-on-the-myenteric-plexus-innervation-and-glia-in-the-murine-distal-colon
#19
Vanesa Stojanovska, Rachel M McQuade, Sarah Miller, Kulmira Nurgali
Oxaliplatin (platinum-based chemotherapeutic agent) is a first-line treatment of colorectal malignancies; its use associates with peripheral neuropathies and gastrointestinal side effects. These gastrointestinal dysfunctions might be due to toxic effects of oxaliplatin on the intestinal innervation and glia. Male Balb/c mice received intraperitoneal injections of sterile water or oxaliplatin (3 mg/kg/d) triweekly for 2 weeks. Colon tissues were collected for immunohistochemical assessment at day 14. The density of sensory, adrenergic, and cholinergic nerve fibers labeled with calcitonin gene-related peptide (CGRP), tyrosine hydroxylase (TH), and vesicular acetylcholine transporter (VAChT), respectively, was assessed within the myenteric plexus of the distal colon...
October 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/29737911/immunolocalization-of-advanced-glycation-end-products-mitogen-activated-protein-kinases-and-transforming-growth-factor-%C3%AE-smads-in-pelvic-organ-prolapse
#20
Antonella Vetuschi, Simona Pompili, Anna Gallone, Angela D'Alfonso, Maria Gabriella Carbone, Gaspare Carta, Claudio Festuccia, Eugenio Gaudio, Alessandro Colapietro, Roberta Sferra
Collagen and matrix metalloproteinases (MMP) play a pivotal role in the pathophysiology of Pelvic Organ Prolapse (POP) as a switch between type I and III collagen together with a simultaneous activation of MMPs have been observed in the vaginal wall. The aim of this study was to evaluate the Advanced Glycation End (AGE) products, ERK1/2 and transforming growth factor (TGF)-β/Smad pathway expression in muscularis propria in women with POP compared with control patients. We examined 20 patients with POP and 10 control patients treated for uterine fibromatosis...
September 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
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