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Iranian Biomedical Journal

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https://www.readbyqxmd.com/read/29776310/preparation-and-evaluation-of-ribonuclease-resistant-viral-hiv-rna-standards-based-on-armored-rna-technology
#1
Mohammad Gholami, Mehrdad Ravanshad, Kazem Baesi, Siamak M. Samiee, Negin Hosseini Rozbahani, Minoo Mohraz
BAckground: The human immunodeficiency virus type 1 (HIV-1) is an infectious viral agent that gradually extinguishes the immune system, resulting in acquired immune deficiency syndrome (AIDS). The aim of this study was to construct an RNA-positive control based on armored (AR) RNA technology, using HIV-1 RNA as a model. Methods: The MS2 maturase, a coat protein gene (at positions 1765 to 1787) and HIV-1 pol gene were cloned into pET-32a plasmid. The prepared plasmid was transformed into Escherichia coli strain BL2 (DE3), and the expression of the construct was induced by 1 mM of isopropyl-L-thio-D-galactopyranoside (IPTG) at 37 °C for 16 h to obtain the fabricated AR RNA...
May 19, 2018: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/29753316/differential-expression-profile-of-zfx-variants-discriminates-breast-cancer-subtypes
#2
Fatemeh Pourkeramati, Malek Hossein Asadi, Shahryar Shakeri, Alireza Farsinejad
Background: ZFX is a transcriptional regulator in embryonic stem cells that plays an important role in pluripotency and self-renewal. ZFX is widely expressed in pluripotent stem cells and is down-regulated during differentiation of embryonic stem cells. ZFX has five different variants that encode three different protein isoforms. While several reports have determined the overexpression of ZFX in a variety of somatic cancers, the expression of ZFX-spliced variants in cancer cells is not well-understood...
May 13, 2018: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/29707938/novel-lama2-gene-mutations-associated-with-merosin-deficient-congenital-muscular-dystrophy
#3
Feyzollah Hashemi-Gorji, Vahid Reza Yassaee, Parisa Dashti, Mohammad Miryounesi
Background: Merosin-deficient congenital muscular dystrophy (MDC1A) is a rare autosomal recessive genetic disease occurred due to mutations in the LAMA2 gene. This study investigated the molecular genetics of three Iranian MDC1A patients who manifested hypotonia, muscle weakness at birth, elevated levels of creatine kinase, and normal magnetic resonance imaging before the age of six months Methods: Peripheral blood samples were collected from three unrelated patients and their families after obtaining their informed written consents...
April 30, 2018: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/29704890/pik3ca-mutation-analysis-in-iranian-patients-with-gastric-cancer
#4
Mostafa Iranpour, Mahyar Nourian, Sana Saffari, Esmaeil Samizadeh, Mahdieh Mirghafori, Shahrokh Iravani, Soudeh Ghafouri-Fard
BAckground: Aberrant activation of phosphatidylinositol-3 kinases (PI3K)/AKT/mTOR (mammalian target of rapamycin) pathway is a critical event during gastric cancer progression. Selective function of AKT inhibitor AZD5363 in PI3KCA mutant gastric cancer necessitates the assessment of PI3KCA mutations in these patients. Methods: The study included 100 patients with gastric cancer who underwent surgical resection at Imam Reza Hospital, Tehran, Iran, between January 2009 and December 2016...
April 29, 2018: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/29678103/ouantitative-proteomic-analysis-of-cellular-responses-to-a-designed-amino-acid-feed-in-a-monoclonal-antibody-producing-chinese-hamster-ovary-cell-line
#5
Fatemeh Torkashvand, Fereidoun Mahboudi, Manouchehr Vossoughi, Elnaz Fatemi, Seyed Masoud Moosavi Basri, Amir Heydari, Behrouz Vaziri
Background: Chinese hamster ovary (CHO) cell line is considered as the most common cell line in the biopharmaceutical industry because of its capability in performing efficient post-translational modifications and producing the recombinant proteins, which are similar to natural human proteins. The optimization of the upstream process via different feed strategies has a great impact on the target molecule expression and yield. Methods: To determine and understand the molecular events beneath the feed effects on the CHO cell, a label-free quantitative proteomic analysis was applied...
April 21, 2018: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/29524322/detection-of-somatic-mutation-in-exon-12-of-dna-polymerase-%C3%AE-in-ovarian-cancer-tissue-samples
#6
Kalyani Khanra, Indranil Choudhuri, Nandan Bhattacharyya
Background: DNA polymerase β (pol β) is a key enzyme of base excision repair pathway. It is a 1-kb gene consisting of 14 exons. Its catalytic part lies between exon 8 and exon 14. Exon 12 has a role in deoxyribonucleotide triphosphate selection for nucleotide transferase activity. Methods: Genomic DNA was isolated from ovarian carcinoma samples. Single strand conformation polymorphism method was used to detect mutation in genomic DNA. Results: Twenty-four patients of the 152 pair of tumor samples (15...
March 10, 2018: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/29523019/application-of-sparse-linear-discriminant-analysis-and-elastic-net-for-diagnosis-of-iga-nephropathy-statistical-and-biological-viewpoints
#7
Tahereh Mohammadi Majd, Shiva Kalantari, Hadi Raeisi Shahraki, Mohsen Nafar, Afshin Almasi, Shiva Samavat, Mahmoud Parvin, Amirhossein Hashemian
Background: IgA nephropathy (IgAN) is the most common primary glomerulonephritis diagnosed based on renal biopsy. Mesangial IgA deposits along with the proliferation of mesangial cells are the histologic hallmark of IgAN. Non-invasive diagnostic tools may help to prompt diagnosis and therapy. The discovery of potential and reliable urinary biomarkers for diagnosis of IgAN depends on applying robust and suitable models. Applying two multivariate modeling methods on a urine proteomic dataset obtained from IgAN patients, and comparison of the results of these methods were the purpose of this study...
March 10, 2018: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/29510602/evaluation-of-antimycobacterial-and-synergistic-activity-of-plants-selected-based-on-cheminformatic-parameters
#8
Nafise Rahgozar, Gholamreza Bakhshi Khaniki, Soroush Sardari
Background: Drug resistance is a major public health problem and a threat to progress made in bovine tuberculosis care and control worldwide. This study aimed at evaluating anti-mycobacterial and synergistic activity of some medicinal plants that were selected by cheminformatics studies against Mycobacterium bovis. Methods: Considering the strong synergistic antimycobacterial action of oleanolic acid in combination with tuberculosis drugs, NCBI database was explored to find the compounds with over 80% similarity to oleanolic acid, called S1...
March 7, 2018: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/29506347/skin-mast-cell-promotion-in-random-skin-flaps-in-rats-using-bone-marrow-mesenchymal-stem-cells-and-amniotic-membrane
#9
Farzaneh Chehelcheraghi, Abolfazl Abbaszadeh, Magid Tavafi
Background: Skin flap procedures are employed in plastic surgery, but failure can lead to necrosis of the flap. Studies have used bone marrow mesenchymal stem cells (BM-MSCs) to improve flap viability. BM-MSCs and acellular amniotic membrane (AAM) have been introduced as alternatives. The objective of this study was to evaluate the effect of BM-MSCs and AAM on mast cells of random skin flaps (RSF) in rats. Methods: RSFs (80 × 30 mm) were created on 40 rats that were randomly assigned to one of four groups, including (I) AAM, (II) BM-MSCs, (III) BM-MSCs/AAM, and (IV) saline (control)...
March 6, 2018: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/29706061/molecular-signaling-in-tumorigenesis-of-gastric-cancer
#10
Fatemeh Molaei, Mohammad Mahdi Forghanifard, Yasaman Fahim, Mohammad Reza Abbaszadegan
Gastric cancer (GC) is regarded as the fifth most common cancer and the third cause of cancer-related deaths worldwide. Mechanism of GC pathogenesis is still unclear and relies on multiple factors, including environmental and genetic characteristics. One of the most important environmental factors of GC occurrence is infection with Helicobacter pylori that is classified as class one carcinogens. Dysregulation of several genes and pathways play an essential role during gastric carcinogenesis. Dysregulation of developmental pathways such as Wnt/β-catenin signaling, Hedgehog signaling, Hippo pathway, Notch signaling, nuclear factor-kB, and epidermal growth factor receptor have been found in GC...
July 2018: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/29704891/an-association-study-between-longitudinal-changes-of-leukocyte-telomere-and-the-risk-of-azoospermia-in-a-population-of-iranian-infertile-men
#11
Hamed Heidary, Farkhondeh Pouresmaeili, Reza Mirfakhraie, Mir Davood Omrani, Hamid Ghaedi, Zahra Fazeli, Shadi Sayban, Soudeh Ghafouri-Fard, Eznollah Azargashb, Fazlollah Shokri
Background: Telomeres are evolutionary, specialized terminal structures at the ends of eukaryotic chromosomes containing TTAGGG repeats in human. Several human diseases have been known to be associated with dramatic changes in telomere length. The aim of the present study was to assess the correlation between the relative leukocyte telomere length (LTL) and infertility in a group of Iranian azoospermic males. Methods: : In this case-control pilot study, relative telomere length (RTL) of peripheral blood leukocytes from a total of 30 idiopathic non-obstructive azoospermic males and 30 healthy fertile males was evaluated using real-time PCR...
July 2018: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/29482316/study-break-possible-diagnostic-improvement-for-cutaneous-leishmaniasis-is-it-achievable
#12
Yasaman Taslimi, Sima Rafati
No abstract text is available yet for this article.
July 2018: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/29101913/study-break-future-of-triple-negative-breast-cancer-can-immunotherapy-treat-this-deadly-subtype-of-breast-cancer
#13
Mona Salimi
No abstract text is available yet for this article.
March 2018: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28952291/high-resolution-hla-a-typing-in-normal-iranian-population
#14
Azadeh Hadadianpour, Mohammad Hasan Samiee Aref, Sirous Zeinali
Background: Human leukocyte antigen (HLA) gene is a highly polymorphic region. HLA typing is required to match patients and donors for transplantation; therefore, development of HLA registries is necessary for finding HLA match donors. HLA system is highly informative, and numerous studies have been conducted on HLA allele distribution in different populations. Methods: In this study, 100 unrelated Iranian individuals were typed for HLA-A locus using sequence-based typing method...
March 2018: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28915725/distribution-of-rotavirus-genotypes-ccirculating-in-ahvaz-iran-in-2016
#15
Azarakhsh Azaran, Manoochehr Makvandi, Ali Teimoori, Saeedeh Ebrahimi, Farzad Heydari, Roya Nikfar
Background: Group A rotavirus (RVA) mainly causes acute gastroenteritis, exclusively in young children in developing countries. The prevalence and determination of the molecular epidemiology of rotavirus genotypes will determine the dominant rotavirus genotypes in the region and provide a strategy for the development of appropriate vaccines. Methods: A total of 100 fecal samples were collected from children below five years with acute gastroenteritis who referred to Aboozar Children’s Hospital of Ahvaz city during October 2015 to March 2016...
March 2018: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28806866/molecular-screening-and-single-nucleotide-polymorphism-typing-of-molluscum-contagiosum-virus-mcv-from-genital-specimens-between-2012-and-2015
#16
Sedigheh Taghinezhad-S, Amir Hossein Mohseni, Hossein Keyvani, Narges Ghobadi
Background: The present study is the first comprehensive report of the Molluscum contagiosum virus (MCV) in Iran based on the molecular technique for differentiation and typing of the MCV1 and MCV2. Methods: Patients were diagnosed as having tumor-like genital warts less than 5 mm in diameter, and HIV seronegative samples were chosen for this cross-sectional study. TaqMan real-time PCR was used to identify MCV following clinical examination. Typing of the MCV-positive specimens was performed in the SNP A27451G region of MC021L gene...
March 2018: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28800701/a-new-indole-derivative-decreased-sall4-gene-expression-in-acute-promyelocytic-leukemia-cell-line-nb4
#17
Zahra Sheikhrezaei, Parisa Heydari, Alireza Farsinezhad, Ahmad Fatemi, Soudeh Khanamani Falahati-Pour, Shokoofeh Darakhshan, Mojgan Noroozi Karimabad, Ali Darekordi, Hossein Khorramdelazad, Gholamhossein Hassanshahi
Background: Acute myeloblastic leukemia (AML) is a clonal disorder due to bone marrow failure and uncontrolled proliferation of myeloid lineage. Acute promyelocytic leukemia (APL) is a subtype of AML. Heterocyclic compounds, such as indole, are considered as attractive candidates for cancer therapy, due to their abundance in nature and known biological activity. Sal-like protein (SALL4) is a zinc finger transcription factor involving in the multi-potency of stem cells, in the NB4 cell line...
March 2018: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28755654/effects-of-bone-marrow-mesenchymal-stem-cells-conditioned-medium-on-tibial-partial-osteotomy-model-of-fracture-healing-in-hypothyroidism-rats
#18
Niloofar Sefati, Mohsen Norouzian, Hojjat-Allah Abbaszadeh, Mohammad-Amin Abdollahifar, Abdollah Amini, Mohammad Bagheri, Arefeh Aryan, Fatemeh Fadaei Fathabady
Background: Hypothyroidism is associated with dysfunction of the bone turnover with reduced osteoblastic bone formation and osteoclastic bone resorption. Mesenchyme stem cells (MSCs) secrete various factors and cytokines that may stimulate bone regeneration. The aim of this study was to determine the effects of MSCs-conditioned medium (CM) in hypothyroidism male rats after inducing bone defect. Methods: : In this study, 24 male rats were randomly assigned to three groups: (I) hypothyroidism+bone defect (HYPO), (II) hypothyroidism+bone defect+CM (HYPO+CM), and (III) no hypothyroidism+bone defect (control)...
March 2018: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28734274/genetic-analysis-of-iranian-patients-with-familial-hypercholesterolemia
#19
Mahdis Ekrami, Maryam Torabi, Soudeh Ghafouri-Fard, Javad Mowla, Bahram Mohammad Soltani, Feyzollah Hashemi-Gorji, Zahra Mohebbi, Mohammad Miryounesi
Background: Familial hypercholesterolemia (FH) is a frequent autosomal dominant disorder of lipoprotein metabolism. This disorder is generally caused by mutations in low-density lipoprotein receptor (LDLR), apolipoprotein B 100 (APOB), and proprotein convertase subtilisin/kexin type 9 (PCSK9) genes. In the present study, we aimed at identifying the common LDLR and APOB gene mutations in an Iranian population. Methods: Eighty unrelated Iranian patients with FH entered the study, based on Simon Broome diagnostic criteria...
March 2018: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28734273/association-between-thrombophilic-genes-polymorphisms-and-recurrent-pregnancy-loss-susceptibility-in-the-iranian-population-a-systematic-review-and-meta-analysis
#20
Mahdieh Kamali, Sedigheh Hantoushzadeh, Sedigheh Borna, Hossein Neamatzadeh, Mahta Mazaheri, Mahmood Noori-Shadkam, Fatemeh Haghighi
Background: Studies have indicated that thrombophilic genes polymorphisms are associated with recurrent pregnancy loss (RPL) in the Iranian population. However, the results from these studies remained inconsistent and inconclusive. The aim of this systematic review and meta-analysis was to evaluate the precise association between thrombophilic genes polymorphisms (MTHFR C677T, MTHFR A1298C, Prothrombin G20210A, FVL G1691A, and PAI-1 4G/5G) and RPL risk in the Iranian population. Method: Electronic databases of PubMed, Web of Science, Google Scholar, and ISC were searched for eligible articles published up to April 1, 2017...
March 2018: Iranian Biomedical Journal
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