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Iranian Biomedical Journal

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https://www.readbyqxmd.com/read/29986553/a-survey-of-the-common-mutations-and-ivs8-tn-polymorphism-of-cystic-fibrosis-transmembrane-conductance-regulator-gene-in-infertile-men-with-nonobstructive-azoospermia-and-cbavd-in-iranian-population
#1
Fatemeh Asadi, Reza Mirfakhraie, Farzaneh Mirzajani, Azam Khedri
Background: Studies have revealed a strong association between mutations of CFTR gene and the congenital bilateral absence of the vas deferens (CBAVD), but the role of this gene in other types of male infertility is still unclear. The purpose of the this study was to investigate the frequency of the most common mutations of the CFTR gene (DF508, G542X, N1303K, G551D, and W1282X) in a population of infertile men with nonobstructive azoospermia (NOA) and CBAVD in Iran. Methods: Blood samples were obtained from 50 NOA, 50 CBAVD, and 100 normal males (control)...
July 10, 2018: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/29883990/assessment-of-cannabinoids-agonist-and-antagonist-in-invasion-potential-of-k562-cancer-cells
#2
Fatemeh Gholizadeh, Mohammad Hossein Ghahremani, Shima Aliebrahimi, Amir Shadboorestan, Seyed Nasser Ostad
Background: The prominent hallmark of malignancies is the metastatic spread of cancer cells. Recent studies have reported that the nature of invasive cells could be changed after this phenomenon, causing chemotherapy resistance. It has been demonstrated that the up-regulated expression of matrix metalloproteinase (MMP) 2/MMP-9, as a metastasis biomarker, can fortify the metastatic potential of leukemia. Furthermore, investigations have confirmed the inhibitory effect of cannabinoid and endocannabinoid on the proliferation of cancer cells in vitro and in vivo...
June 9, 2018: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/29859516/up-regulation-of-mir-21-mir-25-mir-93-and-mir-106b-in-gastric-cancer
#3
Pegah LArki, Alireza Ahadi, Ali Zare, Shahriar Tarighi, Mahrokh Zaheri, Mojgan Souri, Mohammad Reza Zali, Hamid Ghaedi, Mir Davood Omrani
Background: Differential expression profile of microRNAs (miRNAs) could be a diagnosis signature for the monitoring of gastric cancer (GC) progression. In this study, we focus on the comparison of expression levels of miR-21, miR-25, miR-93, miR-106b, and miR-375 during the sequential pattern of GC development, including normal gastric, gastric dysplasia, and GC sample. Methods: We used SYBR Green-based quantitative-PCR to quantify miRNAs expression. Results: Our analysis revealed the increased expression levels of miR-21 (p = 0...
June 3, 2018: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/29843204/differential-connectivity-in-colorectal-cancer-gene-expression-network
#4
Fereshteh Izadi
Background: Colorectal cancer (CRC) is one of the challenging types of cancers; thus, exploring effective biomarkers related to colorectal could lead to significant progresses toward the treatment of this disease. Methods: In the present study, CRC gene expression datasets have been reanalyzed. Mutual differentially expressed genes across 294 normal mucosa and adjacent tumoral samples were then utilized in order to build two independent transcriptional regulatory networks...
May 30, 2018: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/29803203/the-effects-of-lactobacillus-casei-on-glycemic-response-serum-sirtuin1-and-fetuin-a-levels-in-patients-with-type-2-diabetes-mellitus-a-randomized-controlled-trial
#5
Leila Khalili, Beitullah Alipour, Mohammad Asghari Jafar-Abadi, Ismail Faraji, Tohid Hassanalilou, Mehran Mesgari Abbasi, Elnaz Vaghef-Mehrabany, Mahmood Alizadeh Sani
Background: Type 2 diabetes mellitus (T2DM) is related to the gut microbiota with numerous molecular mechanisms. Modulating the gut microbiota by probiotics could be effective in management of T2DM. The aim of the present trial was to evaluate the effect of Lactobacillus casei on glycemic control and serum sirtuin1 (SIRT1) and fetuin-A in patients with T2DM. Methods: Forty patients with T2DM (n = 20 for each group) were divided into intervention (probiotic) and placebo groups...
May 27, 2018: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/29803202/effects-of-major-epigenetic-factors-on-systemic-lupus-erythematosus
#6
Shirin Farivar, Fateme Shaabanpour Aghamaleki
The pathogenesis of systemic lupus erythematosus (SLE) is influenced by both genetic factors and epigenetic modifications; the latter is a result of exposure to various environmental factors. Epigenetic modifications affect gene expression and alter cellular functions without modifying the genomic sequences. CpG-DNA methylation, histone modifications, and miRNAs are the main epigenetic factors of gene regulation. In SLE, global and gene-specific DNA methylation changes have been demonstrated to occur in CD4+ T-cells...
May 27, 2018: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/29802698/study-break-lice-humans-and-microbes
#7
(no author information available yet)
No abstract text is available yet for this article.
May 26, 2018: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/29776310/preparation-and-evaluation-of-ribonuclease-resistant-viral-hiv-rna-standards-based-on-armored-rna-technology
#8
Mohammad Gholami, Mehrdad Ravanshad, Kazem Baesi, Siamak M. Samiee, Negin Hosseini Rozbahani, Minoo Mohraz
BAckground: The human immunodeficiency virus type 1 (HIV-1) is an infectious viral agent that gradually extinguishes the immune system, resulting in acquired immune deficiency syndrome (AIDS). The aim of this study was to construct an RNA-positive control based on armored (AR) RNA technology, using HIV-1 RNA as a model. Methods: The MS2 maturase, a coat protein gene (at positions 1765 to 1787) and HIV-1 pol gene were cloned into pET-32a plasmid. The prepared plasmid was transformed into Escherichia coli strain BL2 (DE3), and the expression of the construct was induced by 1 mM of isopropyl-L-thio-D-galactopyranoside (IPTG) at 37 °C for 16 h to obtain the fabricated AR RNA...
May 19, 2018: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/29753316/differential-expression-profile-of-zfx-variants-discriminates-breast-cancer-subtypes
#9
Fatemeh Pourkeramati, Malek Hossein Asadi, Shahryar Shakeri, Alireza Farsinejad
Background: ZFX is a transcriptional regulator in embryonic stem cells that plays an important role in pluripotency and self-renewal. ZFX is widely expressed in pluripotent stem cells and is down-regulated during differentiation of embryonic stem cells. ZFX has five different variants that encode three different protein isoforms. While several reports have determined the overexpression of ZFX in a variety of somatic cancers, the expression of ZFX-spliced variants in cancer cells is not well-understood...
May 13, 2018: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/29707938/novel-lama2-gene-mutations-associated-with-merosin-deficient-congenital-muscular-dystrophy
#10
Feyzollah Hashemi-Gorji, Vahid Reza Yassaee, Parisa Dashti, Mohammad Miryounesi
Background: Merosin-deficient congenital muscular dystrophy (MDC1A) is a rare autosomal recessive genetic disease occurred due to mutations in the LAMA2 gene. This study investigated the molecular genetics of three Iranian MDC1A patients who manifested hypotonia, muscle weakness at birth, elevated levels of creatine kinase, and normal magnetic resonance imaging before the age of six months Methods: Peripheral blood samples were collected from three unrelated patients and their families after obtaining their informed written consents...
April 30, 2018: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/29704890/pik3ca-mutation-analysis-in-iranian-patients-with-gastric-cancer
#11
Mostafa Iranpour, Mahyar Nourian, Sana Saffari, Esmaeil Samizadeh, Mahdieh Mirghafori, Shahrokh Iravani, Soudeh Ghafouri-Fard
BAckground: Aberrant activation of phosphatidylinositol-3 kinases (PI3K)/AKT/mTOR (mammalian target of rapamycin) pathway is a critical event during gastric cancer progression. Selective function of AKT inhibitor AZD5363 in PI3KCA mutant gastric cancer necessitates the assessment of PI3KCA mutations in these patients. Methods: The study included 100 patients with gastric cancer who underwent surgical resection at Imam Reza Hospital, Tehran, Iran, between January 2009 and December 2016...
April 29, 2018: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/29678103/ouantitative-proteomic-analysis-of-cellular-responses-to-a-designed-amino-acid-feed-in-a-monoclonal-antibody-producing-chinese-hamster-ovary-cell-line
#12
Fatemeh Torkashvand, Fereidoun Mahboudi, Manouchehr Vossoughi, Elnaz Fatemi, Seyed Masoud Moosavi Basri, Amir Heydari, Behrouz Vaziri
Background: Chinese hamster ovary (CHO) cell line is considered as the most common cell line in the biopharmaceutical industry because of its capability in performing efficient post-translational modifications and producing the recombinant proteins, which are similar to natural human proteins. The optimization of the upstream process via different feed strategies has a great impact on the target molecule expression and yield. Methods: To determine and understand the molecular events beneath the feed effects on the CHO cell, a label-free quantitative proteomic analysis was applied...
April 21, 2018: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/29706061/molecular-signaling-in-tumorigenesis-of-gastric-cancer
#13
Fatemeh Molaei, Mohammad Mahdi Forghanifard, Yasaman Fahim, Mohammad Reza Abbaszadegan
Gastric cancer (GC) is regarded as the fifth most common cancer and the third cause of cancer-related deaths worldwide. Mechanism of GC pathogenesis is still unclear and relies on multiple factors, including environmental and genetic characteristics. One of the most important environmental factors of GC occurrence is infection with Helicobacter pylori that is classified as class one carcinogens. Dysregulation of several genes and pathways play an essential role during gastric carcinogenesis. Dysregulation of developmental pathways such as Wnt/β-catenin signaling, Hedgehog signaling, Hippo pathway, Notch signaling, nuclear factor-kB, and epidermal growth factor receptor have been found in GC...
July 2018: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/29704891/an-association-study-between-longitudinal-changes-of-leukocyte-telomere-and-the-risk-of-azoospermia-in-a-population-of-iranian-infertile-men
#14
Hamed Heidary, Farkhondeh Pouresmaeili, Reza Mirfakhraie, Mir Davood Omrani, Hamid Ghaedi, Zahra Fazeli, Shadi Sayban, Soudeh Ghafouri-Fard, Eznollah Azargashb, Fazlollah Shokri
Background: Telomeres are evolutionary, specialized terminal structures at the ends of eukaryotic chromosomes containing TTAGGG repeats in human. Several human diseases have been known to be associated with dramatic changes in telomere length. The aim of the present study was to assess the correlation between the relative leukocyte telomere length (LTL) and infertility in a group of Iranian azoospermic males. Methods: : In this case-control pilot study, relative telomere length (RTL) of peripheral blood leukocytes from a total of 30 idiopathic non-obstructive azoospermic males and 30 healthy fertile males was evaluated using real-time PCR...
July 2018: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/29482316/study-break-possible-diagnostic-improvement-for-cutaneous-leishmaniasis-is-it-achievable
#15
Yasaman Taslimi, Sima Rafati
No abstract text is available yet for this article.
July 2018: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/29397043/study-of-tnp1-tekt1-and-plzf-genes-expression-during-an-in-vitro-three-dimensional-neonatal-male-mice-testis-culture
#16
Ahmad Alrahel, Mansoureh Movahedin, Zohre Mazaheri, Fardin Amidi
Background: In vitro spermatogenesis has a long research history beginning in the early 20th century. This organ culture method was therefore abandoned, and alternative cell culture methods were chosen by many researchers. Here, whether Tnp1, Tekt1, and Plzf, which play a crucial role in spermatogenesis, can be expressed during testis organ culture was assessed. Methods: Testes of 10 mouse pups were first removed, and the testis tissue was then separated into smaller pieces of seminiferous tubules...
July 2018: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/29132205/secretome-of-aggregated-embryonic-stem-cell-derived-mesenchymal-stem-cell-modulates-the-release-of-inflammatory-factors-in-lipopolysaccharide-induced-peripheral-blood-mononuclear-cells
#17
Nastaran Mohammadi Ghahhari, Faezeh Maghsood, Saeed Jahandideh, Majid Lotfinia, Shirin Lak, Behrooz Johari, Asaad Azarnezhad, Mehdi Kadivar
Background: Bone marrow mesenchymal stem cells (BM-MSCs) have emerged as a potential therapy for various inflammatory diseases. Because of some limitations, several recent studies have suggested the use of embryonic stem cell-derived MSCs (ESC-MSCs) as an alternative for BM-MSCs. Some of the therapeutic effects of the ESC-MSCs are related to the secretion of a broad array of cytokines and growth factors, known as secretome. Harnessing this secretome for therapeutic applications requires the optimization of production of secretary molecules...
July 2018: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/29108398/blockade-of-p75-neurotrophin-receptor-reverses-irritability-and-anxiety-related-behaviors-in-a-rat-model-of-status-epilepticus
#18
Soraya Mehrabi, Mahyar Janahamdi, Mohammad Taghi Joghataie, Mahmood Barati, Mohsen Marzban, Mahmoudreza Hadjighassem, Maryam Farahmandfar
Background: Many recent epidemiological studies have shown that epileptic patients are more likely suffer from depression, anxiety, and irritability. However, the cellular mechanisms of epilepsy-induced psychotic behaviors are not fully elucidated. Neurotrophin receptors have been suggested to be involved in epilepsy and also in psychiatric disorders. Up-regulation of p75NTR expression and activation of p75NTR signalling cascades after the seizure have been shown, but the role of the p75 receptor in epilepsy-induced psychotic behaviors has not been documented so far...
July 2018: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/29031245/decrease-in-cavity-size-and-oligodendrocyte-cell-death-using-neurosphere-derived-oligodendrocyte-like-cells-in-spinal-cord-contusion-model
#19
Hojjat Allah Abbaszadeh, Taki Tiraihi, Yousef Sadeghi, Ali Reza Delshad, Majid Sadeghizadeh, Taher Taheri, Ali Noori-Zadeh
Background: Oligodendrocyte cell death is among the important features of spinal cord injury, which appears within 15 min and occurs intensely for 4 h after injury, in the rat spinal contusion model. Accordingly, the number of oligodendrocytes progressively reduced within 24 h after injury. Administration of oligodendrocyte-like cells (OLCs) into the lesion area is one of the approaches to counterbalance this condition. Methods: Bone marrow stromal cells were transdifferentiated into neurospheres and then into neural stem cells and later were differentiated into OLCs using triiodothyronine and transplanted into the spinal cord contusion rats...
July 2018: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/29031244/development-of-new-recombinant-dgk-antigen-for-diagnosis-of-dirofilaria-immitis-infections-in-dogs-using-elisa-technique-and-its-comparison-to-molecular-methods
#20
Rahmat Solgi, Seyed Mahmoud Sadjjadi, Mehdi Mohebali, Zabihollah Zarei, Majid Golkar, Abbasali Raz
Background: Dirofilaria immitis is a cosmopolitan zoonotic, vector-borne parasite of carnivorous animals causing dirofilariasis in human beings. Common commercial serodiagnostic tests for canine dirofilariasis usually lead to different results in their sensitivity and specificity. The present study reports development of recombinant DgK (rDgK) antigen of D. immitis for accurate immunodiagnosis of D. Immitis-infected dogs using indirect ELISA test. Methods: The rDgK coding sequence was successfully sequenced, codon optimized and cloned into pET-24a(+) expression vector and then expressed in Escherichia coli...
July 2018: Iranian Biomedical Journal
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