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Iranian Biomedical Journal

Alexandr Alexandrovich Basov, Аnna Anatolyevna Elkina, Andrei Alexandrovich Samkov, Nikita Nikolaevich Volchenko, Arcadii Victorovich Moiseev, Liliya Viacheslavovna Fedulova, Mikhail Gennadievich Baryshev, Stepan Sergeevich Dzhimak
Background: This study aimed to evaluate the reaction of organism of laboratory animals on deuterium-depleted drinking diet. To assess the cell energy metabolism, the effect of a liquid medium with different deuterium contents on isolated liver mitochondria of random bred rats and Wistar rats was studied. Methods: This experimental study on the effect of deuterium-depleted drinking water (DDW) on 16-week-old male Wistar rats lasted for four weeks. Energy metabolism of mitochondria was examined through the production of hydrogen peroxide using an Amplex® Red Hydrogen Peroxide/Peroxidase Assay Kit...
September 17, 2018: Iranian Biomedical Journal
Omid Emadian Saravi, Farshad Naghshvar, Zhila Torabizadeh, Somayeh Sheidaei
Background: Cancer stem cells (CSCs) are a group of tumor cells with self-renewal property and differentiation potential. CSCs play a crucial role in malignant progression of several types of tumors. However, what is still controversial is the clinicopathological relationship between Nanog marker and its prognostic value in the patients with breast cancer. The expression of Nanog in the patients with breast cancer and its correlation with clinicopathological prognostic factors was explored in the present study...
September 17, 2018: Iranian Biomedical Journal
Mohammad Mehdi Attarpour Yazdi, Nikky Tofighi, Taraneh Rajaee, Marzieh Ghahremanlou, Ahmad Adeli, Bolhassani Azam, Mohammad Azizi, Noushin Davoudi
Background: The expression of bio-therapeutic proteins in mammalian cells, such as CHO, attains high homogeneity related to post-translational modifications. Although CHO remains the most popular cell line for bestselling biotherapeutic proteins on the market, there are still drawbacks such as expensive culture media, long time line, and high drug cost. Recently, researches on a novel Leishmania protozoan system have confirmed that this low level eukaryote could represent a competitive alternative to the mammalian cell lines...
September 17, 2018: Iranian Biomedical Journal
Soheila Adeli, Maryam Zahmatkesh, Mitra Ansari Dezfouli
Background: Matrix metalloproteinase-9 (MMP-9) expression has been implicated in molecular mechanisms of neurodegenerative disorders, and its abnormal level has been reported in Alzheimer’s disease (AD). Some protective mechanisms of statins against neurodegeneration might be mediated by the inhibition of MMP-9 expression. Here, we investigated the effect of simvastatin, on the hippocampal MMP-9 expression in the context of AD. Methods: We examined the influence of three-week simvastatin (5 mg/kg) administration on hippocampal MMP-9 expression in a rat model of cognitive decline induced by streptozotocin (STZ)...
September 16, 2018: Iranian Biomedical Journal
Hamed Ghadiri, Sana Alavi, Bahareh Dabirmanesh, Kenji Moriyama, Khosro Khajeh, Hisao Masai
No abstract text is available yet for this article.
September 16, 2018: Iranian Biomedical Journal
Parisa Azerang, Vahid Khalaj, Farzad obarfard, Parviz Owlia, Soroush Sardari, Sahar Shahidi
Background: The majority of studies on soil Aspergillus concern the isolation and characterization of the antimicrobial compounds produced by this organism. Our previous studies indicated an isolated Aspergillus strain soil to be of interest, and this subject is further studied here. Methods: Soil samples of various locations in Iran were collected. Extract from Aspergillus sp. culture was obtained using ethyl acetate fractionation. Antimicrobial activity testing was performed using broth microdilution assay against Escherichia coli, Candida albicans, and Staphylococcus aureus microorganisms...
September 16, 2018: Iranian Biomedical Journal
Hassan Asadi, Ehsan Mostafavi
Background: Scientometrics is a discipline that analyzes scientific publications to explore the structure and growth of science. In this work, the quantitative evaluation of the productivity of the Iranian Biomedical Journal (IBJ) is reviewed. Methods: The analysis was done based on a cross-sectional descriptive and analytical scientometric study. Data were collected from PubMed, Scopus, and Scimago Databases (2000-2017). Scopus and Scimago were used for data search and feature analysis...
August 19, 2018: Iranian Biomedical Journal
Zarin Sharifnia, Mojgan Bandehpour, Bahram Kazemi, Nosratollah Zarghami
Background: Hepatitis C virus (HCV) is a ‎blood-borne pathogen, resulting in liver cirrhosis and liver cancer. Despite of many efforts in development of treatments for HCV, no vaccine has been licensed yet. The purpose of this study was ‎to design and prepare a specific mRNA, without 5' cap and poly (A) tail transcribed in vitro capable of coding core protein and also to determine its functionality. Methods: Candidate mRNA was prepared by in vitro transcription of the designed construct consisting of ‎‎5ʹ and 3ʹ untranslated regions of heat shock proteins 70 (hsp70) mRNA, T7 promoter, internal ribosome entry site (IRES) sequences of eIF4G related to human dendritic cells (DCs), and the ‎Core gene of HCV...
July 30, 2018: Iranian Biomedical Journal
Saeid Afshar, Sepideh Afshar, Emily Warden, Hamed Manochehri, Massoud Saidijam
Background: The early diagnosis of colorectal cancer (CRC) is associated with improved survival rates, and development of novel non-invasive, sensitive, and specific diagnostic tests is highly demanded. The objective of this paper was to identify commonly circulating microRNA (miRNA) biomarkers for use in CRC diagnosis. Methods: For this purpose, an artificial neural network (ANN) model was proposed. Among miRNAs retrieved from the Gene Expression Omnibus dataset, four miRNAs with the best miRNA score were selected by ANN units...
July 30, 2018: Iranian Biomedical Journal
Zahra Zendehbad, Pantea Izadi, Abdolreza Daraei, Mir Saeed Yekaninejad, Nahid Nafissi, Nasim Younosi, Ghasemali Khorasani, Javad Tavakkoly Bazzaz
Background: Young age at First Full Term Pregnancy (FFTP) is an important factor in breast cancer risk reduction. It is postulated that this protective effect is the result of stable molecular signatures imprinted by physiological process of pregnancy, but the molecular mechanism of this protective role is unclear. The aim of the current study was to identify the effect of early FFTP on methylation status of FOXA1 gene body. FOXA1 is an essential transcription factor for mammary gland development and estrogen responsiveness of breast tissue...
July 28, 2018: Iranian Biomedical Journal
Zohreh Hojati, Elaheh Soleimanpour, Seyed-Morteza Javadirad, Mohammad Hossein Nasr-Esfahani
Background: KDM3A is a key epigenetic regulator that is expressed in the testis and is required for packaging and condensation of sperm chromatin. To this point, the association of the KDM3A gene and infertility has not been studied in human. The aim of this study was to screen any new mutation in KDM3A gene to explore more details of human male infertility. Methods: In this work, 150 infertile men (oligozoospermia and azoospermia) and 150 normal healthy fathers were studied...
July 28, 2018: Iranian Biomedical Journal
Reza Jafari, Naime Majidi Zolbanin, Jafar Majidi, Fatemeh Atyabi, Mehdi Yousefi, Farhad Jadidi-Niaragh, Leili Aghebati-Maleki, Dariush Shanehbandi, Mohammad-Sadegh Soltani Zangbar, Houshang Rafatpanah
Background: Targeted co-delivery of siRNA and a chemotherapeutic drug is an attractive approach to cancer drug design and treatment. This study was carried out to design an anti-Mucin 1 aptamer (Apt)-conjugated chitosan nanoparticle (NP) for targeted co-delivery of insulin-like growth factor receptor 1 (IGF-1R) siRNA and docetaxel (DTX) to SKBR3 cells. Methods: Characterization of nano-drugs, cellular uptake of NPs, cell viability, and gene expression studies were evaluated based on metastatic breast cancer cells...
July 25, 2018: Iranian Biomedical Journal
Mehdi Alikhani, Ebrahim Shafaie, Esmat Mirabzadeh Ardakani, Maryam Esmaeili, Samaneh Saberi, Mojgan Hatefi, Marjan Mohammadi
Background: Quantitation of Helicobacter pylori (Hp) in the gastric tissue is essential for assessment of vaccination/therapeutic regimens. Materials & Results: Here, the inhibitory effect of mouse gastric DNA (MgDNA) on amplification of Hp genomic DNA (HpDNA) was evaluated by spiking HpDNA with serial dilutions of MgDNA, which yielded concentrations of >10 ng/µl and >0.63-10 ng/µl of MgDNA, as inhibition and interference zones, respectively. Mice were then inoculated with varying doses of Hp and assessed at the inhibition-free concentration of 0...
July 25, 2018: Iranian Biomedical Journal
Emily Vivianne Freitas da Silva, Daniela Micheline dos Santos, Liliane da Rocha Bonatto, Victor Gustavo Balera Brito, Sandra Helena Penha de Oliveira, Marcelo Coelho Goiato
Background: This study was undertaken to analyze if different preparation and exposure periods of eluates from ocular prosthesis acrylic resin influence the cytotoxicity for conjunctival cells. Methods: Twenty-four acrylic resin specimens were divided, according to the period of eluate exposure to Chang conjunctival cells (24 and 72 hours). Eluates were prepared in four different ways: 24, 48, and 72 hours of resin specimen immersion in medium and 24 hours of immersion in water followed by 24 hours of immersion in medium...
July 17, 2018: Iranian Biomedical Journal
Fatemeh Asadi, Reza Mirfakhraie, Farzaneh Mirzajani, Azam Khedri
Background: Studies have revealed a strong association between mutations of CFTR gene and the congenital bilateral absence of the vas deferens (CBAVD), but the role of this gene in other types of male infertility is still unclear. The purpose of the this study was to investigate the frequency of the most common mutations of the CFTR gene (DF508, G542X, N1303K, G551D, and W1282X) in a population of infertile men with nonobstructive azoospermia (NOA) and CBAVD in Iran. Methods: Blood samples were obtained from 50 NOA, 50 CBAVD, and 100 normal males (control)...
July 10, 2018: Iranian Biomedical Journal
Shirin Farivar, Fateme Shaabanpour Aghamaleki
The pathogenesis of systemic lupus erythematosus (SLE) is influenced by both genetic factors and epigenetic modifications; the latter is a result of exposure to various environmental factors. Epigenetic modifications affect gene expression and alter cellular functions without modifying the genomic sequences. CpG-DNA methylation, histone modifications, and miRNAs are the main epigenetic factors of gene regulation. In SLE, global and gene-specific DNA methylation changes have been demonstrated to occur in CD4+ T-cells...
September 2018: Iranian Biomedical Journal
Saied Reza Naddaf
No abstract text is available yet for this article.
September 2018: Iranian Biomedical Journal
Kalyani Khanra, Indranil Choudhuri, Nandan Bhattacharyya
Background: DNA polymerase β (pol β) is a key enzyme of base excision repair pathway. It is a 1-kb gene consisting of 14 exons. Its catalytic part lies between exon 8 and exon 14. Exon 12 has a role in deoxyribonucleotide triphosphate selection for nucleotide transferase activity. Methods: Genomic DNA was isolated from ovarian carcinoma samples. Single strand conformation polymorphism method was used to detect mutation in genomic DNA. Results: Twenty-four patients of the 152 pair of tumor samples (15...
September 2018: Iranian Biomedical Journal
Farzaneh Chehelcheraghi, Abolfazl Abbaszadeh, Magid Tavafi
Background: Skin flap procedures are employed in plastic surgery, but failure can lead to necrosis of the flap. Studies have used bone marrow mesenchymal stem cells (BM-MSCs) to improve flap viability. BM-MSCs and acellular amniotic membrane (AAM) have been introduced as alternatives. The objective of this study was to evaluate the effect of BM-MSCs and AAM on mast cells of random skin flaps (RSF) in rats. Methods: RSFs (80 × 30 mm) were created on 40 rats that were randomly assigned to one of four groups, including (I) AAM, (II) BM-MSCs, (III) BM-MSCs/AAM, and (IV) saline (control)...
September 2018: Iranian Biomedical Journal
Seyedeh Maryam Seyedolmohadessin, Mohammad Taghi Akbari, Zahra Nourmohammadi, Abbas Basiri, Gholamreza Pourmand
Background: Prostate cancer is the second form of cancer among men worldwide. For early cancer detection, we should identify tumors in initial stages before the physical signs become visible. The present study aims to evaluate the diagnostic value of cell-free DNA (cfDNA), its comparison with prostate-specific antigen (PSA) level in prostate cancer screening and also in patients with localized prostate cancer, metastatic form, and benign prostatic hyperplasia (BPH). Methods: The participants of this study were selected from 126 patients with genitourinary symptoms suspected prostate cancer, rising PSA, and/or abnormal rectal examination results and 10 healthy subjects as controls...
September 2018: Iranian Biomedical Journal
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