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Iranian Biomedical Journal

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https://www.readbyqxmd.com/read/28806866/molecular-screening-and-single-nucleotide-polymorphism-typing-of-molluscum-contagiosum-virus-mcv-from-genital-specimens-between-2012-and-2015
#1
Sedigheh Taghinezhad-S, Amir Hossein Mohseni, Hossein Keyvani, Narges Ghobadi
Background: The present study is the first comprehensive report of the Molluscum contagiosum virus (MCV) in Iran based on the molecular technique for differentiation and typing of the MCV1 and MCV2. Methods: Patients were diagnosed as having tumor-like genital warts less than 5 mm in diameter, and HIV seronegative samples were chosen for this cross-sectional study. TaqMan real-time PCR was used to identify MCV following clinical examination. Typing of the MCV-positive specimens was performed in the SNP A27451G region of MC021L gene...
August 15, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28806865/glucose-sensitive-holographic-bio-sensors-fundamentals-and-applications
#2
Marjan Heidari, Bahareh Dabirmanesh, Hossein Mahdavi, Khosro Khajeh
Nowadays sensors and especially biosensors play an important role in medical diagnosis and detection of food and environment contaminations. Biosensors’ facilities have been improved significantly by using technologies such as surface plasmon resonance, microfluidics, laser, and electrochemistry. These technologies are now available on chips in micro- and nano-scale and are capable of measuring the quantity of more than one analyte at once. One branch of these sensors is the holographic (bio)sensor. Reflection holographic sensors, which are optical devices fabricated based on Bragg’s law, can diffract light with specific wavelength as they absorb or release special ions or molecule depending on their polymer structure...
August 15, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28800701/a-new-indole-derivative-decreased-sall4-gene-expression-in-acute-promyelocytic-leukemia-cell-line-nb4
#3
Zahra Sheikhrezaei, Parisa Heydari, Alireza Farsinezhad, Ahmad Fatemi, Soudeh Khanamani Falahati-Pour, Shokoofeh Darakhshan, Mojgan Noroozi Karimabad, Ali Darekordi, Hossein Khorramdelazad, Gholamhossein Hassanshahi
Background: Acute myeloblastic leukemia (AML) is a clonal disorder due to bone marrow failure and uncontrolled proliferation of myeloid lineage. Acute promyelocytic leukemia (APL) is a subtype of AML. Heterocyclic compounds, such as indole, are considered as attractive candidates for cancer therapy, due to their abundance in nature and known biological activity. Sal-like protein (SALL4) is a zinc finger transcription factor involving in the multi-potency of stem cells, in the NB4 cell line...
August 12, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28766326/a-review-of-brucellosis-in-iran-epidemiology-risk-factors-diagnosis-control-and-prevention
#4
Maryam Golshani, Saeid Buozari
Brucellosis caused by species of Brucella is among the most prevalent zoonoses with the annual incidence of half a million cases globally. Most parts of Iran are endemic for brucellosis, and the annual incidence of the human and animal brucellosis is still high. At present, there is no safe and protective human vaccine against brucellosis, and the only preventive strategy is animal vaccination, which harbors significant disadvantages. Considering the identification of many immunogenic proteins in Brucella, several studies have recently been performed to evaluate the vaccine potency of such antigens as a new subunit vaccine candidate...
August 2, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28755655/therapeutic-effects-of-iranian-herbal-extracts-against-trichomonas-vaginalis
#5
Zohreh Fakhrieh Kashan, Mahdi Delavari, Mohsen Arbabi, Hossein Hooshyar
Trichomonas vaginalis is a flagellated parasite affecting about 276 million people annually worldwide. Tricomoniasis is associated with different complications in pregnant women and infants. 5'-nitroimidazole derivatives (metronidazole, ornidazole, and tinidazole) are FDA approved drugs recommended for trichomoniasis treatment. Treatment with metronidazole 5'-nitroimidazole derivatives is associated with many side effects, and drug resistance to metronidazole has been reported in some cases. Recently, many attempts have been made to evaluate the effects of plants on causative agents of vaginal infections...
July 30, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28755654/effects-of-bone-marrow-mesenchymal-stem-cells-conditioned-medium-on-tibial-partial-osteotomy-model-of-fracture-healing-in-hypothyroidism-rats
#6
Niloofar Sefati, Mohsen Norouzian, Hojjat-Allah Abbaszadeh, Mohammad-Amin Abdollahifar, Abdollah Amini, Mohammad Bagheri, Arefeh Aryan, Fatemeh Fadaei Fathabady
Background: Hypothyroidism is associated with dysfunction of the bone turnover with reduced osteoblastic bone formation and osteoclastic bone resorption. Mesenchyme stem cells (MSCs) secrete various factors and cytokines that may stimulate bone regeneration. The aim of this study was to determine the effects of MSCs-conditioned medium (CM) in hypothyroidism male rats after inducing bone defect. Methods: : In this study, 24 male rats were randomly assigned to three groups: (I) hypothyroidism+bone defect (HYPO), (II) hypothyroidism+bone defect+CM (HYPO+CM), and (III) no hypothyroidism+bone defect (control)...
July 30, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28734275/low-level-of-autophagy-related-gene-10-atg10-expression-in-the-6-hydroxydopamine-rat-model-of-parkinson-s-disease
#7
Marzieh Shams Nooraei, Ali Noori-Zadeh, Shahram Darabi, Farzad Rajaei, Zohreh Golmohammadi, Hojjat Allah Abbaszadeh
Background: Autophagy is a mechanism disassembling the damaged organelles from the cell. This study attempted to examine the expression of several autophagy-related genes in Parkinson’s disease (PD) rat model. Methods: The male Wistar rats were divided into three groups as control, sham, and lesion. In the latter group, the PD rat model was induced by the injection of 6-hydroxydopamine in the striatum.The behavioral test was conducted one (baseline) and four weeks after the surgery through apomorphine hydrochloride...
July 23, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28734274/genetic-analysis-of-iranian-patients-with-familial-hypercholesterolemia
#8
Mahdis Ekrami, Maryam Torabi, Soudeh Ghafouri-Fard, Javad Mowla, Bahram Mohammad Soltani, Feyzollah Hashemi-Gorji, Zahra Mohebbi, Mohammad Miryounesi
Background: Familial hypercholesterolemia (FH) is a frequent autosomal dominant disorder of lipoprotein metabolism. This disorder is generally caused by mutations in low-density lipoprotein receptor (LDLR), apolipoprotein B 100 (APOB), and proprotein convertase subtilisin/kexin type 9 (PCSK9) genes. In the present study, we aimed at identifying the common LDLR and APOB gene mutations in an Iranian population. Methods: Eighty unrelated Iranian patients with FH entered the study, based on Simon Broome diagnostic criteria...
July 23, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28734273/association-between-thrombophilic-genes-polymorphisms-and-recurrent-pregnancy-loss-susceptibility-in-the-iranian-population-a-systematic-review-and-meta-analysis
#9
Mahdieh Kamali, Sedigheh Hantoushzadeh, Sedigheh Borna, Hossein Neamatzadeh, Mahta Mazaheri, Mahmood Noori-Shadkam, Fatemeh Haghighi
Background: Studies have indicated that thrombophilic genes polymorphisms are associated with recurrent pregnancy loss (RPL) in the Iranian population. However, the results from these studies remained inconsistent and inconclusive. The aim of this systematic review and meta-analysis was to evaluate the precise association between thrombophilic genes polymorphisms (MTHFR C677T, MTHFR A1298C, Prothrombin G20210A, FVL G1691A, and PAI-1 4G/5G) and RPL risk in the Iranian population. Method: Electronic databases of PubMed, Web of Science, Google Scholar, and ISC were searched for eligible articles published up to April 1, 2017...
July 23, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28710847/effect-of-passage-number-and-culture-time-on-the-expression-and-activity-of-insulin-degrading-enzyme-in-caco-2-cells
#10
Taiebeh Mohammadi Farsani, Elahe Motevaseli, Nadia Neyazi, Mohammad Reza Khorramizadeh, Elaheh Zafarvahedian, Mohammad Hossein Ghahremani
Background: Insulin-degrading enzyme (IDE) is a conserved zinc metallopeptidase. Here, we have evaluated the effect of passage number and culture time on IDE expression and activity in colorectal adenocarcinoma cells (Caco-2) cells. Methods: Caco-2 cells were cultured with different passage ranges of 5-15, 25-35, 52-63 for 48, 72, and 120 hours. Subsequently, IDE expression and enzyme activity were assessed by Western blot analysis and fluorescent assay, respectively...
July 15, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28693095/analysis-of-nsp4-gene-and-its-association-with-genotyping-of-rotavirus-group-a-in-stool-samples
#11
Ali Teimoori, Mehrab Nejati, Saeedeh Ebrahimi, Manoochehr Makvandi, Milad Zandi, Azarakhsh Azaran
Background: Non-structural protein 4 (NSP4) is a critical protein for rotavirus (RV) replication and assembly. This protein has multiple domains and motifs that predispose its function and activity. NSP4 has a sequence divergence in human and animal RVs. Recently, 14 genotypes (E1-E14) of NSP4 have been identified, and E1 and E2 have been shown to be the most common genotypes in human. Methods: The gene and protein sequence of NSP4 in RV-positive samples were inspected with the aim of NSP4 genotyping and variation analysis in viroporin and other domains...
July 11, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28689385/expression-optimization-of-anti-cd22-scfv-apoptin-fusion-protein-using-experimental-design-methodology
#12
Solmaz Agha Amiri, Najmeh Zarei, Somayeh Enayati, Mohammad Azizi, Vahid Khalaj, Soraya Shahhosseini
Background: Design of experiments is a rapid and cost-effective approach for optimization of recombinant protein production process. In our previous study, we generated a potent dual-acting fusion protein, anti-CD22 scFv-apoptin, to target B-cell malignant cell lines. In the present investigation, we report the effect of different variables on the expression levels of this fusion protein. Methods: : Four variables (cell optical density at induction, IPTG concentration, induction temperature, and induction time) were tested using experimental design...
July 10, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28688377/no-role-of-herpes-simplex-virus-type-2-hsv-2-infection-on-hiv-progression-in-na%C3%A3-ve-hiv-patients
#13
Minoo Mohraz, Arezoo Aghakhani, Saeedeh Moayedi-Nia, Mohammad Banifazl, Alireza Janbakhsh, Setareh Mamishi, Afsaneh Karami, Anahita Bavand, Pegah Mirzapour, Amitis Ramezani
Background: Herpes simplex virus type 2 (HSV-2) is a common infection in human immunodeficiency virus (HIV) patients and may accelerate HIV progression by rising HIV viral load and decreasing CD4 count. However, the available data regarding the influence of HSV-2 seropositivity on HIV progression in HIV individuals are inconclusive. Therefore, we aimed to determine HSV-2 seroprevalence in naïve HIV patients and normal controls and also investigate the relation of HIV viral load and CD4 count with HSV-2 seropositivity...
July 9, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28688376/molecular-identification-and-antifungal-susceptibility-pattern-of-non-albicans-candida-species-isolated-from-vulvovaginal-candidiasis
#14
Ziba Abbasi Nejat, Shirin Farahyar, Mehraban Falahati, Mahtab Ashrafi Khozani, Aga Fateme Hosseini, Azamsadat Faiazy, Masoome Ekhtiari, Saeideh Hashemi-Hafshenjani
Background: Vulvovaginal candidiasis (VVC) is an important problem due to Candida spp. The aim of this study was molecular identification, phylogenetic analysis, and evaluation of antifungal susceptibility of non-albicans Candida isolates from VVC. Methods: Vaginal secretion samples were collected from 550 vaginitis patients at Sayyad Shirazi Medical and Educational Center of Gorgan (Golestan Province, Iran) from May to October 2015. Samples were analyzed using conventional mycological and molecular approaches...
July 9, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28646827/immunization-of-c57bl-6-mice-with-gra2-combined-with-mpl-conferred-partial-immune-protection-against-toxoplasma-gondii
#15
Jalal Babaie, Samira Amiri, Robab Homayoun, Ebrahim Azimi, Reyhaneh Mohabati, Mahboobe Berizi, M. Reza Sadaie, Majid Golkar
Background: We have previously reported that immunization with GRA2 antigen of Toxoplasma gondii induces protective immunity in CBA/J (H2k) and BALB/c mice (H2d). We aimed to examine whether immunization of a distinct strain of rodent with recombinant dense granule antigens (GRA2) combined with monophosphorryl lipid A (MPL) adjuvant elicits protective immune response against T. gondii. Methods: C57BL/6 (H2b haplotype) mice were immunized with GRA2, formulated in MPL adjuvant...
June 25, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28646826/comparison-of-brca1-expression-between-triple-negative-and-luminal-breast-tumors
#16
Farzaneh Darbeheshti, Pantea Izadi, Amir Nader Emami Razavi, Mir Saeed Yekaninejad, Javad Tavakkoly Bazzaz
Background: Previous studies have suggested that BRCA1 dysregulation has a role in triple-negative phenotypic manifestation. However, differences of BRCA1 expression, as a tumor suppressor gene, have rarely been investigated between luminal and triple-negative breast tumors. Therefore, the present study attempted to compare the BRCA1 expression in triple-negative with luminal breast tumors and its association with the clinicopathologic characteristics of patients. Methods: BRCA1 expression was evaluated by real-time PCR in 26 triple-negative and 27 luminal breast tumors...
June 25, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28627158/evaluation-of-serum-interleukin-21-and-hla-c1-polymorphism-in-pediatrician-hematopoietic-stem-cell-transplantation-for-early-diagnosis-of-acute-graft-versus-host-disease
#17
N Sehati, P Kokhaei, A Motevalizade Ardekani, R Tootoonchian, F Pak
Background: Allogenic hematopoietic stem cell transplantation (HSCT) is a strategy used for treatment of different malignant diseases. However, success of allo-HSCT can be hampered by graft-versus-host-disease (GVHD). Natural killer (NK) cells may play an important role in activating antigen presenting cells and subsequent activation of T cells. The main purpose of this study was the evaluation of IL-21, as a blood biomarker, for early detection of acute GVHD (aGVHD) in children after HSCT and also the study of human leukocytes antigen (HLA)-C1 polymorphism, as a targeting ligand for NK cells in these patients...
June 19, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28605892/improvement-of-thermal-stability-of-bcg-vaccine
#18
Fatemeh Jahanbakhsh Sefidi, Homan Kaghazian, Gholam Ali Moradli, Seyed Mehdi Hassanzadeh
BAckground: Thermal stability (TS) is a part of the BCG vaccine characterization by which the consistency of process in BCG vaccine production could be confirmed. To enhance the TS of the vaccine, some prevalent stabilizers in different concentrations were added to the final formulation of BCG bulk prior to Freeze-drying process. We found a formulation more effective than the current stabilizer for retaining the higher viability of lyophilized BCG vaccine produced by Pasteur Institute of Iran...
June 13, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28601058/in-vitro-study-of-spions-c595-as-molecular-imaging-probe-for-specific-breast-cancer-mcf-7-cells-detection
#19
Pegah Moradi Khaniabadi, Daryoush Shahbazi-Gahrouei, Amin Malik Shah Abdul Majid, Mohammad Suhaimi Jaafar, Bita Moradi Khaniabadi, Saghar Shahbazi-Gahrouei
Background: Magnetic resonance imaging (MRI) plays an essential role in molecular imaging by delivering the contrast agent into targeted cancer cells. The aim of this study was to evaluate the C595 monoclonal antibody-conjugated superparamagnetic iron oxide nanoparticles (SPIONs-C595) for the detection of breast cancer cell (MCF-7). Methods: The conjugation of monoclonal antibody and nanoparticles was confirmed using X-ray diffraction, transmission electron microscopy, and photon correlation spectroscopy...
June 11, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28558440/chemical-composition-and-antimicrobial-activities-of-iranian-propolis
#20
Houshang Afrouzan, Tahghighi Azar, Sedigheh Zakeri, Es-haghi Ali
Background: With considering the importance of natural products for their remedial and therapeutic value, this research was aimed to analyze the chemical compositions and antimicrobial activity of four propolis samples from different areas of Iran (Chenaran, Taleghan, Morad Beyg, and Kalaleh) with various climates and flora. Methods: ethanolic (70% EtOH) and dichlromethane (DCM) extracts of Iranian propolis were analyzed by gas chromatography-mass spectrometry (GC-MS) methods, and antimicrobial activity was evaluated against Candida albicans, Escherichia coli, and Staphylococcus aureus using disk diffusion antimicrobial method...
May 31, 2017: Iranian Biomedical Journal
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