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Iranian Biomedical Journal

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https://www.readbyqxmd.com/read/28710847/effect-of-passage-number-and-culture-time-on-the-expression-and-activity-of-insulin-degrading-enzyme-in-caco-2-cells
#1
Taiebeh Mohammadi Farsani, Elahe Motevaseli, Nadia Neyazi, Mohammad Reza Khorramizadeh, Elaheh Zafarvahedian, Mohammad Hossein Ghahremani
Background: Insulin-degrading enzyme (IDE) is a conserved zinc metallopeptidase. Here, we have evaluated the effect of passage number and culture time on IDE expression and activity in colorectal adenocarcinoma cells (Caco-2) cells. Methods: Caco-2 cells were cultured with different passage ranges of 5-15, 25-35, 52-63 for 48, 72, and 120 hours. Subsequently, IDE expression and enzyme activity were assessed by Western blot analysis and fluorescent assay, respectively...
July 15, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28693095/analysis-of-nsp4-gene-and-its-association-with-genotyping-of-rotavirus-group-a-in-stool-samples
#2
Ali Teimoori, Mehrab Nejati, Saeedeh Ebrahimi, Manoochehr Makvandi, Milad Zandi, Azarakhsh Azaran
Background: Non-structural protein 4 (NSP4) is a critical protein for rotavirus (RV) replication and assembly. This protein has multiple domains and motifs that predispose its function and activity. NSP4 has a sequence divergence in human and animal RVs. Recently, 14 genotypes (E1-E14) of NSP4 have been identified, and E1 and E2 have been shown to be the most common genotypes in human. Methods: The gene and protein sequence of NSP4 in RV-positive samples were inspected with the aim of NSP4 genotyping and variation analysis in viroporin and other domains...
July 11, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28689385/expression-optimization-of-anti-cd22-scfv-apoptin-fusion-protein-using-experimental-design-methodology
#3
Solmaz Agha Amiri, Najmeh Zarei, Somayeh Enayati, Mohammad Azizi, Vahid Khalaj, Soraya Shahhosseini
Background: Design of experiments is a rapid and cost-effective approach for optimization of recombinant protein production process. In our previous study, we generated a potent dual-acting fusion protein, anti-CD22 scFv-apoptin, to target B-cell malignant cell lines. In the present investigation, we report the effect of different variables on the expression levels of this fusion protein. Methods: : Four variables (cell optical density at induction, IPTG concentration, induction temperature, and induction time) were tested using experimental design...
July 10, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28688377/no-role-of-herpes-simplex-virus-type-2-hsv-2-infection-on-hiv-progression-in-na%C3%A3-ve-hiv-patients
#4
Minoo Mohraz, Arezoo Aghakhani, Saeedeh Moayedi-Nia, Mohammad Banifazl, Alireza Janbakhsh, Setareh Mamishi, Afsaneh Karami, Anahita Bavand, Pegah Mirzapour, Amitis Ramezani
Background: Herpes simplex virus type 2 (HSV-2) is a common infection in human immunodeficiency virus (HIV) patients and may accelerate HIV progression by rising HIV viral load and decreasing CD4 count. However, the available data regarding the influence of HSV-2 seropositivity on HIV progression in HIV individuals are inconclusive. Therefore, we aimed to determine HSV-2 seroprevalence in naïve HIV patients and normal controls and also investigate the relation of HIV viral load and CD4 count with HSV-2 seropositivity...
July 9, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28688376/molecular-identification-and-antifungal-susceptibility-pattern-of-non-albicans-candida-species-isolated-from-vulvovaginal-candidiasis
#5
Ziba Abbasi Nejat, Shirin Farahyar, Mehraban Falahati, Mahtab Ashrafi Khozani, Aga Fateme Hosseini, Azamsadat Faiazy, Masoome Ekhtiari, Saeideh Hashemi-Hafshenjani
Background: Vulvovaginal candidiasis (VVC) is an important problem due to Candida spp. The aim of this study was molecular identification, phylogenetic analysis, and evaluation of antifungal susceptibility of non-albicans Candida isolates from VVC. Methods: Vaginal secretion samples were collected from 550 vaginitis patients at Sayyad Shirazi Medical and Educational Center of Gorgan (Golestan Province, Iran) from May to October 2015. Samples were analyzed using conventional mycological and molecular approaches...
July 9, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28646827/immunization-of-c57bl-6-mice-with-gra2-combined-with-mpl-conferred-partial-immune-protection-against-toxoplasma-gondii
#6
Jalal Babaie, Samira Amiri, Robab Homayoun, Ebrahim Azimi, Reyhaneh Mohabati, Mahboobe Berizi, M. Reza Sadaie, Majid Golkar
Background: We have previously reported that immunization with GRA2 antigen of Toxoplasma gondii induces protective immunity in CBA/J (H2k) and BALB/c mice (H2d). We aimed to examine whether immunization of a distinct strain of rodent with recombinant dense granule antigens (GRA2) combined with monophosphorryl lipid A (MPL) adjuvant elicits protective immune response against T. gondii. Methods: C57BL/6 (H2b haplotype) mice were immunized with GRA2, formulated in MPL adjuvant...
June 25, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28646826/comparison-of-brca1-expression-between-triple-negative-and-luminal-breast-tumors
#7
Farzaneh Darbeheshti, Pantea Izadi, Amir Nader Emami Razavi, Mir Saeed Yekaninejad, Javad Tavakkoly Bazzaz
Background: Previous studies have suggested that BRCA1 dysregulation has a role in triple-negative phenotypic manifestation. However, differences of BRCA1 expression, as a tumor suppressor gene, have rarely been investigated between luminal and triple-negative breast tumors. Therefore, the present study attempted to compare the BRCA1 expression in triple-negative with luminal breast tumors and its association with the clinicopathologic characteristics of patients. Methods: BRCA1 expression was evaluated by real-time PCR in 26 triple-negative and 27 luminal breast tumors...
June 25, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28627158/evaluation-of-serum-interleukin-21-and-hla-c1-polymorphism-in-pediatrician-hematopoietic-stem-cell-transplantation-for-early-diagnosis-of-acute-graft-versus-host-disease
#8
N Sehati, P Kokhaei, A Motevalizade Ardekani, R Tootoonchian, F Pak
Background: Allogenic hematopoietic stem cell transplantation (HSCT) is a strategy used for treatment of different malignant diseases. However, success of allo-HSCT can be hampered by graft-versus-host-disease (GVHD). Natural killer (NK) cells may play an important role in activating antigen presenting cells and subsequent activation of T cells. The main purpose of this study was the evaluation of IL-21, as a blood biomarker, for early detection of acute GVHD (aGVHD) in children after HSCT and also the study of human leukocytes antigen (HLA)-C1 polymorphism, as a targeting ligand for NK cells in these patients...
June 19, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28605892/improvement-of-thermal-stability-of-bcg-vaccine
#9
Fatemeh Jahanbakhsh Sefidi, Homan Kaghazian, Gholam Ali Moradli, Seyed Mehdi Hassanzadeh
BAckground: Thermal stability (TS) is a part of the BCG vaccine characterization by which consistency of process in BCG vaccine production could be confirmed. To enhance the TS of the vaccine, some prevalent stabilizers in different concentration were added to the final formulation of BCG bulk prior to Freeze-drying process. We found a formulation more effective than the current stabilizer for retaining the higher viability of lyophilized BCG vaccine produced by Pasteur Institute of Iran...
June 13, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28601058/in-vitro-study-of-spions-c595-as-molecular-imaging-probe-for-specific-breast-cancer-mcf-7-cells-detection
#10
Pegah Moradi Khaniabadi, Daryoush Shahbazi-Gahrouei, Amin Malik Shah Abdul Majid, Mohammad Suhaimi Jaafar, Bita Moradi Khaniabadi, Saghar Shahbazi-Gahrouei
Background: Magnetic resonance imaging (MRI) plays an essential role in molecular imaging by delivering the contrast agent into targeted cancer cells. The aim of this study was to evaluate the C595 monoclonal antibody-conjugated superparamagnetic iron oxide nanoparticles (SPIONs-C595) for the detection of breast cancer cell (MCF-7). Methods: The conjugation of monoclonal antibody and nanoparticles was confirmed using X-ray diffraction, transmission electron microscopy, and photon correlation spectroscopy...
June 11, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28558440/chemical-composition-and-antimicrobial-activities-of-iranian-propolis
#11
Houshang Afrouzan, Tahghighi Azar, Sedigheh Zakeri, Es-haghi Ali
Background: With considering the importance of natural products for their remedial and therapeutic value, this research was aimed to analyze the chemical compositions and antimicrobial activity of four propolis samples from different areas of Iran (Chenaran, Taleghan, Morad Beyg, and Kalaleh) with various climates and flora. Methods: ethanolic (70% EtOH) and dichlromethane (DCM) extracts of Iranian propolis were analyzed by gas chromatography-mass spectrometry (GC-MS) methods, and antimicrobial activity was evaluated against Candida albicans, Escherichia coli, and Staphylococcus aureus using disk diffusion antimicrobial method...
May 31, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28558439/accuracy-of-soluble-endoglin-for-diagnosis-of-preeclampsia-and-its-severity
#12
Pooneh Nikuei, Minoo Rajaei, Kianoosh Malekzadeh, Azim Nejatizadeh, Fatemeh Mohseni, Ali AtashAbParvar
Background: The use of biomarkers for diagnosis of Preeclampsia (PE), a life-threatening pregnancy disorder, could reduce serious complications of this disease. In this study, we investigated dysregulation of endoglin (Eng) expression and diagnostic accuracy of soluble endoglin (sEng) in PE patients. Methods: For this case-control study, 26 mild and 15 severe preeclamptic women along with 20 normotensive controls were recruited. The expression level of Eng (the co-receptor of TGF-β1) was evaluated using qRT-PCR...
May 31, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28558438/accuracy-of-soluble-endoglin-for-diagnosis-of-preeclampsia-and-its-severity
#13
Pooneh Nikuei, Minoo Rajaei, Kianoosh Malekzadeh, Azim Nejatizadeh, Fatemeh Mohseni, Ali AtashAbParvar
Background: The use of biomarkers for diagnosis of Preeclampsia (PE), a life-threatening pregnancy disorder, could reduce serious complications of this disease. In this study, we investigated dysregulation of endoglin (Eng) expression and diagnostic accuracy of soluble endoglin (sEng) in PE patients. Methods: For this case-control study, 26 mild and 15 severe preeclamptic women along with 20 normotensive controls were recruited. The expression level of Eng (the co-receptor TGF-β1) was evaluated using qRT-PCR...
May 31, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28558437/precision-medicine-a-new-revolution-in-healthcare-system
#14
Babak Arjmand, Mohammad Abdollahi, Bagher Larijani
Every human being is different based on genetics, lifestyle, and environmental factors. Novel medical technologies have become more precise owing to molecular information, including genomics, transcriptomics, proteomics, metabolomics, etc. The “omics” technologies have opened up new horizons for healthcare systems, enabling them to prevent and/or diagnose diseases more precisely, as well as to find the most effective treatments with fewer adverse events. Recently, the term ‘precision medicine’ (PM), focusing on molecular-based medicine, has been introduced...
May 31, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28555492/equilibrium-isotherm-kinetic-modeling-optimization-and-characterization-studies-of-cadmium-adsorption-by-surface-engineered-escherichia-coli
#15
Vida Tafakori, Reza Zadmard, Fatemeh Tabandeh, Mohammad Ali Amoozegar, Gholamreza Ahamadian
Background: Amongst the methods that remove heavy metals from environment, biosorption approaches have received increased attention because of their environmentally friendly and cost-effective feature, as well as their superior performances. Methods: In the present study, we investigated the ability of a surface-engineered Escherichia coli, carrying the cyanobacterial metallothionein on the cell surface, in the removal of Ca (II) from solution under different experimental conditions...
May 30, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28551944/misconduct-in-research-and-publication
#16
LETTER
Viroj Wiwanitkit
Dear Editor, I read the recent publication on “Misconduct in Research and Publication” with great interest[1]. I agree that misconduct in research and publication is not uncommon. Nevertheless, it is rarely mentioned. In fact, there are many incorrect conceptions among researchers on publication ethics. The milder examples are attempts to report only the “positive outcomes”, textual or data recycling as well as figure cropping and modification. These problems can be seen in published work of many academic members regardless of nationality or seniority...
May 29, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28532144/physically-targeted-intravenous-polyurethane-nanoparticles-for-controlled-release-of-atorvastatin-calcium
#17
Behnaz Sadat Eftekhari, Akbar Karkhaneh, Ali Alizadeh
Background: Intravenous drug delivery is an advantageous choice for rapid administration, immediate drug effect, and avoidance of first-pass metabolism in oral drug delivery. In this study, the synthesis, formulation, and characterization of atorvastatin-loaded polyurethane (PU) nanoparticles were investigated for intravenous route of administration. Methods: First, PU was synthesized and characterized. Second, nanoparticles were prepared in four different ratios of drug to polymer through two different techniques including emulsion-diffusion and single-emulsion...
May 23, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28480695/expression-study-and-clinical-correlations-of-myc-and-ccat2-in-breast-cancer-patients
#18
Shaghayegh Sarrafzadeh, Lobat Geranpayeh, Behnoosh Tasharrofi, Mohammad Soudyab, Elahe Nikpayam, Mostafa Iranpour, Reza Mirfakhraie, Jalal Gharesouran, Somayyeh Ghafouri-Fard, Soudeh Ghafouri-Fard
Background: Colon cancer-associated transcript 2 (CCAT2) is a newly recognized lncRNA transcribed from the 8q24 genomic region. It functions as an oncogene in various types of cancers including breast cancer, in which it affects Wnt/β-catenin pathway. Previous studies have shown a putative interaction between this lncRNA and MYC proto-oncogene. Methods: In the current study, we evaluated the expression of CCAT2 in breast cancer tissues with regards to the expression of its target MYC...
May 8, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28460491/homozygosity-mapping-and-targeted-sanger-sequencing-identifies-three-novel-crb1-crumbs-homologue-1-mutations-in-iranian-retinal-degeneration-families
#19
Mohammad Ghofrani, Mahin Yahyaei, Han G. Brunner, Frans P.M. Cremers, Morteza Movasat, Muhammad Imran Khan, Mohammad Keramatipour
Background: Inherited retinal diseases (IRDs) are a group of genetic disorders with high degrees of clinical, genetic and allelic heterogeneity. IRDs generally show progressive retinal cell death resulting in gradual vision loss. IRDs constitute a broad spectrum of disorders including retinitis pigmentosa and Leber congenital amaurosis. In this study, we performed genotyping studies to identify the underlying mutations in three Iranian families. Method: Having employed homozygosity mapping and Sanger sequencing, we identified the underlying mutations in the crumbs homologue 1 gene...
May 2, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28460428/e6-specific-detection-and-typing-of-human-papillomaviruses-in-oral-cavity-specimens-from-iranian-patients
#20
Hadi Razavi Nikoo, Abdollah Ardebili, Mehrdad Ravanshad, Fatemeh Rezaei, Ali Teimoori, Sayyad Khanizadeh, Mohammad Hassan Pouriayevali, Mehdi Ajorloo
Background: Detection and quantification of human Papillomavirus (HPV) genome in oral carcinoma plays an important role in diagnosis, as well as implications for progression of disease. Methods: We evaluated tissues from 50 esopharyngeal cancers collected from different regions of Iran for HPV E6 using the two type-specific primers sets. E6 gene of HPV genotypes was amplified by specific primers. The sensitivity of PCR assay was analyzed and determined using HPV-DNA-containing plasmids...
May 2, 2017: Iranian Biomedical Journal
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