journal
MENU ▼
Read by QxMD icon Read
search

Iranian Biomedical Journal

journal
https://www.readbyqxmd.com/read/28532144/physically-targeted-intravenous-polyurethane-nanoparticles-for-controlled-release-of-atorvastatin-calcium
#1
Behnaz Sadat Eftekhari, Akbar Karkhaneh, Ali Alizadeh
Background: Intravenous drug delivery is an advantageous choice for rapid administration, immediate drug effect, and avoidance of first-pass metabolism in oral drug delivery. In this study, the synthesis, formulation, and characterization of atorvastatin-loaded polyurethane (PU) nanoparticles were investigated for intravenous route of administration. Methods: First, PU was synthesized and characterized. Second, nanoparticles were prepared in four different ratios of drug to polymer through two different techniques including emulsion-diffusion and single-emulsion...
May 23, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28480695/expression-study-and-clinical-correlations-of-myc-and-ccat2-in-breast-cancer-patients
#2
Shaghayegh Sarrafzadeh, Lobat Geranpayeh, Behnoosh Tasharrofi, Mohammad Soudyab, Elahe Nikpayam, Mostafa Iranpour, Reza Mirfakhraie, Jalal Gharesouran, Somayyeh Ghafouri-Fard, Soudeh Ghafouri-Fard
Background: Colon cancer-associated transcript 2 (CCAT2) is a newly recognized lncRNA transcribed from the 8q24 genomic region. It functions as an oncogene in various types of cancers including breast cancer, in which it affects Wnt/β-catenin pathway. Previous studies have shown a putative interaction between this lncRNA and MYC proto-oncogene. Methods: In the current study, we evaluated the expression of CCAT2 in breast cancer tissues with regards to the expression of its target MYC...
May 8, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28460491/homozygosity-mapping-and-targeted-sanger-sequencing-identifies-three-novel-crb1-crumbs-homologue-1-mutations-in-iranian-retinal-degeneration-families
#3
Mohammad Ghofrani, Mahin Yahyaei, Han G. Brunner, Frans P.M. Cremers, Morteza Movasat, Muhammad Imran Khan, Mohammad Keramatipour
Background: Inherited retinal diseases (IRDs) are a group of genetic disorders with high degrees of clinical, genetic and allelic heterogeneity. IRDs generally show progressive retinal cell death resulting in gradual vision loss. IRDs constitute a broad spectrum of disorders including retinitis pigmentosa and Leber congenital amaurosis. In this study, we performed genotyping studies to identify the underlying mutations in three Iranian families. Method: Having employed homozygosity mapping and Sanger sequencing, we identified the underlying mutations in the crumbs homologue 1 gene...
May 2, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28460428/e6-specific-detection-and-typing-of-human-papillomaviruses-in-oral-cavity-specimens-from-iranian-patients
#4
Hadi Razavi Nikoo, Abdollah Ardebili, Mehrdad Ravanshad, Fatemeh Rezaei, Ali Teimoori, Sayyad Khanizadeh, Mohammad Hassan Pouriayevali, Mehdi Ajorloo
Background: Detection and quantification of human Papillomavirus (HPV) genome in oral carcinoma plays an important role in diagnosis, as well as implications for progression of disease. Methods: We evaluated tissues from 50 esopharyngeal cancers collected from different regions of Iran for HPV E6 using the two type-specific primers sets. E6 gene of HPV genotypes was amplified by specific primers. The sensitivity of PCR assay was analyzed and determined using HPV-DNA-containing plasmids...
May 2, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28459147/synergistic-effects-of-arsenic-trioxide-and-radiation-triggering-of-intrinsic-pathway-of-apoptosis
#5
Kave Moloudi, Ali Neshasteriz, Arshad Hosseini, Nazila Eyvazzadeh, Mehdi Shomali, Samira Eynali, Elahe Mirzaei, Asaad Azarnezhad
Background: Arsenic trioxide (ATO) has been reported as an effective anti-cancer and a US Food and Drug Administration (FDA) approved drug for treatment of some cancers. The aim of this study is to determine the underlying apoptosis molecular and cellular mechanisms of ATO in the presence or absence of ionizing radiation (IR) in vitro in the glioblastoma multiforme (GBM) cell line, U87MG. Methods: Cells were treated by different concentrations of ATO either in presence or absence of IR...
May 1, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28454485/cheminformatics-based-anticoagulant-study-of-traditionally-used-medicinal-plants
#6
Mahdi Alikhani Pour, Soroush Sardari, Ali Eslamifar, Abid Azhar, Mohammad Rezvani, Milad Nazari
Backgroung: Medicinal plants, as a complementary medicine, have been used to treat various diseases since ancient times. These plants have numerous beneficial applications and are the source of certain conventional drugs. In diseases such as stroke and ischemia, which are caused by several factors, abnormal coagulation is an important causative factor. Accordingly, novel and effective therapies such as herbal remedies should be practiced to prevent such lethal diseases. Methods: Using the available databases such as Google Scholar and PubMed, the previously reported anticoagulant compounds and plants possessing anticoagulant activity were identified and collected in two separate lists...
April 29, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28433004/plackett-burman-design-and-response-surface-optimization-of-medium-trace-nutrients-for-glycolipopeptide-biosurfactant-production
#7
Maurice George Ekpenyong, Sylvester Peter Antai, Atim David Asitok, Bassey Offiong Ekpo
Background: A glycolipopeptide biosurfactant produced by Pseudomonas aeruginosa strain IKW1 reduced the surface tension of fermentation broth from 71.31 to 24.62 dynes/cm at a critical micelle concentration of 20.80 mg/L. The compound proved suitable for applications in emulsion stabilization in food, as well as in cosmetic and pharmaceutical formulations. Method: In the present study, Plackett-Burman design (PBD) and response surface method (RSM) were employed to screen and optimize concentrations of trace nutrients in the fermentation medium, to increase surfactant yield...
April 23, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28431466/next-generation-sequencing-reveals-one-novel-missense-mutation-in-col1a2-gene-in-an-iranian-family-with-osteogenesis-imperfecta
#8
Farah Talebi, Farideh Ghanbari Mardasi, Mohammadi Asl Javad, Bavarsad Amir Hooshang, Salehi Kambo Masoumeh
BAckground: Osteogenesis imperfecta (OI) is a clinically and genetically heterogeneous disorder characterized by bone loss and bone fragility. The aim of this study was to investigate the variants of three genes involved in the pathogenesis of OI. Methods: Molecular genetic analyses were performed for COL1A1, COL1A2, and CRTAP genes in an Iranian family with OI. The DNA samples were analyzed by next-generation sequencing (NGS) gene panel and Sanger sequencing. Results: Five different variants were identified in COL1A1 and COL1A2, including two variants in COL1A1 and three variants in COL1A2...
April 22, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28415833/molecular-characterization-and-biodiversity-of-a-putative-chlorotoxin-from-the-iranian-yellow-scorpion-odontobuthus-doriae
#9
Maryam Naderi Soorki, Amir Jalali, Hamid Galehdari
BAckground: Chloride channels have already been over-expressed in the different types of cancer. Chlorotoxins, as the blocking agent of these channels, have been indicated to be an effective drug against tumors. In this study, we characterized a putative chlorotoxin from a cDNA library of the venom glands obtained from the Iranian scorpion Odontobuthus doriae. Methods: A cDNA library was constructed from venom gland transcriptome of six scorpions. The cDNA encoding Odontobuthus doriae chlorotoxin was isolated from the library, and its putative peptide was characterized by some bioinformatics software such as protein blast, SignalP4...
April 18, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28410549/evaluation-of-factors-influencing-antibody-reduction-for-development-of-antibody-drug-conjugates
#10
Meghdad Abdollahpour-Alitappeh, Majid Lotfinia, Sepand Razavi-Vakhshourpour, Saeed Jahandideh, Hamid Najminejad, Koushan Sineh Sepehr, Reza Moazami, Elnaz Shams, Mahdi Habibi-Anbouhi, Mohsen Abolhassani
Background: Reduction/alkylation is one of the leading strategies for the development of antibody drug conjugates (ADCs). Precise control of the reduction process would not only yield a defined number of free thiols per antibody but also result in development of more homogenous conjugates. Methods: In the present study, we investigated the effect of various dithiothreitol (DTT) concentrations, temperature conditions, and DTT exposure times on antibody reduction...
April 15, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28525954/construction-of-various-%C3%AE-34-5-deleted-fluorescent-expressing-oncolytic-herpes-simplex-type-1-ohsv-for-generation-and-isolation-of-hsv-based-vectors
#11
Shahriyar Abdoli, Farzin Roohvand, Ladan Teimoori-Toolabi, Mohammad Ali Shokrgozar, Mina Bahrololoumi, Kayhan Azadmanesh
Background: Oncolytic herpes simplex virus (oHSV)-based vectors lacking γ34.5 gene, are considered as ideal templates to construct efficient vectors for (targeted) cancer gene therapy. Herein, we reported the construction of three single/dually-flourescence labeled and γ34.5-deleted, recombinant HSV-1 vectors for rapid generation and easy selection/isolation of different HSV-Based vectors. Methods: Generation of recombinant viruses was performed with conventional homologous recombination methods using green fluorescent protein (GFP) and BleCherry harboring shuttle vectors...
July 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28525953/human-yeast-hybrids-new-visions-to-genetic-disorders-and-drug-discovery
#12
Najmeh Zarei, Vahid Khalaj
Yeast has been a very helpful organism for centuries, especially with respect to fermentation of sugars and production of bread. However, for an even longer time, yeast has been a distant relative of humans having diverged from a common ancestor, about one billion years ago. More than one third of the yeast genes have human counterparts, despite this evolutionary distance. Yeast and human orthologs perform the same or similar functions. Investigations have demonstrated that 9-92% of the amino acid sequences in similar human and yeast proteins overlap...
July 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28500725/misconduct-in-research-and-publication-a-dilemma-that-is-taking-place
#13
EDITORIAL
Mohammad Hossein Asghari, Milad Moloudizargari, Mohammad Abdollahi
Having considered current reports concerning plagiarisms taking place in the global science community, the authors decided to address the principal reasons, which lead to these illegalities. In recent years, misconduct in research, such as plagiarism, fabrication, falsification, guest author, ghost author, self-citation, etc. have been increasing significantly in scientific papers, proving a lack of commitment to publication ethics among some authors...
July 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/27752182/introducing-a-new-experimental-islet-transplantation-model-using-biomimetic-hydrogel-and-a-simple-high-yield-islet-isolation-technique
#14
Jamal Mohammadi Ayenehdeh, Bahareh Niknam, Seyed Mahmoud Hashemi, Hossein Rahavi, Nima Rezaei, Masoud Soleimani, Nader Tajik
BACKGROUND: Islet transplantation could be an ideal alternative treatment to insulin therapy for type 1 diabetes Mellitus (T1DM). This clinical and experimental field requires a model that covers problems such as requiring a large number of functional and viable islets, the optimal transplantation site, and the prevention of islet dispersion. Hence, the methods of choice for isolation of functional islets and transplantation are crucial. METHODS: The present study has introduced an experimental model that overcomes some critical issues in islet transplantation, including in situ pancreas perfusion by digestive enzymes through common bile duct...
July 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28214454/revolutionizing-tissue-engineering-through-mirroring-cell-niche-and-application-of-natural-compounds
#15
Hosein Shahsavarani, Mohammad Ali Shokrgozar
Considering the high prevalence of severe organ failures due to the cancer, congenital anomaly, or trauma, and the consequent needs for tissue transplantation, deficiencies in tissues and organs are a huge challenge for regenerative medicine at the moment. More than 40 years have passed since the term ‘tissue engineering’ was created as a new therapeutic means, which may overcome the drawbacks involved in the current artificial tissue or organ transplantation. Nevertheless, application of regenerated tissues is still restricted mainly owing to the cost, poor biocompatibility, low bio-functionality, as well as immune rejection...
May 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28187683/proteomics-profiling-of-chimeric-truncated-tissue-plasminogen-activator-producing-chinese-hamster-ovary-cells-cultivated-in-a-chemically-defined-medium-supplemented-with-protein-hydrolysates
#16
Bahareh Azarian, Seyedeh Matin Sajedin, Amin Azimi, Mozhgan Raigani, Behrouz Vaziri, Fatemeh Davami
Background: Culture media enrichment through the addition of protein hydrolysates is beneficial for achieving higher protein expression. Methods: In this study, designing the optimum mixture of four soy and casein-derived hydrolysates was successfully performed by design of experiment and specific productivity increased in all predicted combinations. Protein profile of recombinant CHO (rCHO) cells producing tissue plasminogen activator in a serum-free medium (SFM) supplemented with designed hydrolysate additives was compared to that of rCHO cells cultivated in SFM...
May 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28176518/main-quality-attributes-of-monoclonal-antibodies-and-effect-of-cell-culture-components
#17
REVIEW
Fatemeh Torkashvand, Behrouz Vaziri
The culture media optimization is an inevitable part of upstream process development in therapeutic monoclonal antibodies (mAbs) production. The quality by design (QbD) approach defines the assured quality of the final product through the development stage. An important step in QbD is determination of the main quality attributes. During the media optimization, some of the main quality attributes such as glycosylation pattern, charge variants, aggregates, and low-molecular-weight species, could be significantly altered...
May 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28176517/regulation-of-erg3-erg6-and-erg11-genes-in-antifungal-resistant-isolates-of-candida-parapsilosis
#18
Ensieh Lotfali, Ali Ghajari, Parivash Kordbacheh, Farideh Zaini, Hossein Mirhendi, Rasoul Mohammadi, Fatemeh Noorbakhsh, Sassan Rezaie
Background: Candida parapsilosis is one of the five common strains of yeasts involved in invasive candidiasis. The expression analysis of sterol biosynthesis pathway genes, which are associated with resistance, can assist the better understanding of antifungal resistance mechanisms. Method: The antifungal susceptibility of 120 clinical C. parapsilosis isolates was examined. The changes in the gene expression related to resistance were analyzed. Results: Eight strains were resistant to fluconazole (FLC), itraconazole (ITC), and amphotericin B (AMB)...
May 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28160769/common-genetic-variant-of-insig2-gene-rs7566605-polymorphism-is-associated-with-severe-obesity-in-north-india
#19
Jai Prakash, Balraj Mittal, Srivastava Apurva, Awasthi Shally, Srivastava Pranjal, Srivastava Neena
Background: Obesity is a very common disorder resulting from an imbalance between food intake and energy expenditure, and it has a substantial impact on the development of chronic diseases. The aim of this study was to examine the association of INSIG2 (rs7566605) gene polymorphism with obesity and obesity associated phenotypes in North Indian subjects. Methods: The variants were investigated for association in 642 obese and non-obese individuals. The genotyping of INSIG2 (rs7566605) single nucleotide polymorphism was analyzed by the TaqMan allelic discrimination protocol...
May 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28131109/fabrication-of-chitosan-silk-based-tracheal-scaffold-using-freeze-casting-method
#20
Zeinab Nematollahi, Mohammad Tafazzoli-Shadpour, Ali Zamanian, Amir Seyedsalehi, Shadmehr Mohammad-Behgam, Fariba Ghorbani, Fereshte Mirahmadi
Background: Since the treatments of long tracheal lesions are associated with some limitations, tissue engineered trachea is considered as an alternative option. This study aimed at preparing a composite scaffold, based on natural and synthetic materials for tracheal tissue engineering. Methods: Nine chitosan silk-based scaffolds were fabricated using three freezing rates (0.5, 1, and 2°C/min) and glutaraldehyde (GA) concentrations (0, 0.4, and 0.8 wt%). Samples were characterized, and scaffolds having mechanical properties compatible with those of human trachea and proper biodegradability were selected for chondrocyte cell seeding and subsequent biological assessments...
May 2017: Iranian Biomedical Journal
journal
journal
33084
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"