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Iranian Biomedical Journal

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https://www.readbyqxmd.com/read/28433004/plackett-burman-design-and-response-surface-optimization-of-medium-trace-nutrients-for-glycolipopeptide-biosurfactant-production
#1
Maurice George Ekpenyong, Sylvester Peter Antai, Atim David Asitok, Bassey Offiong Ekpo
Background: A glycolipopeptide biosurfactant produced by Pseudomonas aeruginosa strain IKW1 reduced the surface tension of fermentation broth from 71.31 to 24.62 dynes/cm at a critical micelle concentration of 20.80 mg/L. The compound proved suitable for applications in emulsion stabilization in food, as well as in cosmetic and pharmaceutical formulations. Method: In the present study, Plackett-Burman design (PBD) and response surface method (RSM) were employed to screen and optimize concentrations of trace nutrients in the fermentation medium, to increase surfactant yield...
April 23, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28431466/next-generation-sequencing-reveals-one-novel-missense-mutation-in-col1a2-gene-in-an-iranian-family-with-osteogenesis-imperfecta
#2
Farah Talebi, Farideh Ghanbari Mardasi, Mohammadi Asl Javad, Bavarsad Amir Hooshang, Salehi Kambo Masoumeh
BAckground: Osteogenesis imperfecta (OI) is a clinically and genetically heterogeneous disorder characterized by bone loss and bone fragility. The aim of this study was to investigate the variants of three genes involved in the pathogenesis of OI. Methods: Molecular genetic analyses were performed for COL1A1, COL1A2, and CRTAP genes in an Iranian family with OI. The DNA samples were analyzed by next-generation sequencing (NGS) gene panel and Sanger sequencing. Results: Five different variants were identified in COL1A1 and COL1A2, including two variants in COL1A1 and three variants in COL1A2...
April 22, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28415833/molecular-characterization-and-biodiversity-of-a-putative-chlorotoxin-from-the-iranian-yellow-scorpion-odontobuthus-doriae
#3
Maryam Naderi Soorki, Amir Jalali, Hamid Galehdari
BAckground: Chloride channels have already been over-expressed in the different types of cancer. Chlorotoxins, as the blocking agent of these channels, have been indicated to be an effective drug against tumors. In this study, we characterized a putative chlorotoxin from a cDNA library of the venom glands obtained from the Iranian scorpion Odontobuthus doriae. Methods: A cDNA library was constructed from venom gland transcriptome of six scorpions. The cDNA encoding Odontobuthus doriae chlorotoxin was isolated from the library, and its putative peptide was characterized by some bioinformatics software such as protein blast, SignalP4...
April 18, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28410549/evaluation-of-factors-influencing-antibody-reduction-for-development-of-antibody-drug-conjugates
#4
Meghdad Abdollahpour-Alitappeh, Majid Lotfinia, Sepand Razavi-Vakhshourpour, Saeed Jahandideh, Hamid Najminejad, Koushan Sineh Sepehr, Reza Moazami, Elnaz Shams, Mahdi Habibi-Anbouhi, Mohsen Abolhassani
Background: Reduction/alkylation is one of the leading strategies for the development of antibody drug conjugates (ADCs). Precise control of the reduction process would not only yield a defined number of free thiols per antibody but also result in development of more homogenous conjugates. Methods: In the present study, we investigated the effect of various dithiothreitol (DTT) concentrations, temperature conditions, and DTT exposure times on antibody reduction...
April 15, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28187683/proteomics-profiling-of-chimeric-truncated-tissue-plasminogen-activator-producing-chinese-hamster-ovary-cells-cultivated-in-a-chemically-defined-medium-supplemented-with-protein-hydrolysates
#5
Bahareh Azarian, Seyedeh Matin Sajedin, Amin Azimi, Mozhgan Raigani, Behrouz Vaziri, Fatemeh Davami
Background: Culture media enrichment through the addition of protein hydrolysates is beneficial for achieving higher protein expression. Methods: In this study, designing the optimum mixture of four soy and casein-derived hydrolysates was successfully performed by design of experiment and specific productivity increased in all predicted combinations. Protein profile of recombinant CHO (rCHO) cells producing tissue plasminogen activator in a serum-free medium (SFM) supplemented with designed hydrolysate additives was compared to that of rCHO cells cultivated in SFM...
February 11, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28214454/revolutionizing-tissue-engineering-through-mirroring-cell-niche-and-application-of-natural-compounds
#6
Hosein Shahsavarani, Mohammad Ali Shokrgozar
Considering the high prevalence of severe organ failures due to the cancer, congenital anomaly, or trauma, and the consequent needs for tissue transplantation, deficiencies in tissues and organs are a huge challenge for regenerative medicine at the moment. More than 40 years have passed since the term ‘tissue engineering’ was created as a new therapeutic means, which may overcome the drawbacks involved in the current artificial tissue or organ transplantation. Nevertheless, application of regenerated tissues is still restricted mainly owing to the cost, poor biocompatibility, low bio-functionality, as well as immune rejection...
February 8, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28176518/main-quality-attributes-of-monoclonal-antibodies-and-effect-of-cell-culture-components
#7
Fatemeh Torkashvand, Behrouz Vaziri
The culture media optimization is an inevitable part of upstream process development in therapeutic monoclonal antibodies (mAbs) production. The quality by design (QbD) approach defines the assured quality of the final product through the development stage. An important step in QbD is determination of the main quality attributes. During the media optimization, some of the main quality attributes such as glycosylation pattern, charge variants, aggregates, and low-molecular-weight species, could be significantly altered...
February 8, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28176517/regulation-of-erg3-erg6-and-erg11-genes-in-antifungal-resistant-isolates-of-candida-parapsilosis
#8
Ensieh Lotfali, Ali Ghajari, Parivash Kordbacheh, Farideh Zaini, Hossein Mirhendi, Rasoul Mohammadi, Fatemeh Noorbakhsh, Sassan Rezaie
Background: Candida parapsilosis is one of the five common strains of yeasts involved in invasive candidiasis. The expression analysis of sterol biosynthesis pathway genes, which are associated with resistance, can assist the better understanding of antifungal resistance mechanisms. Method: The antifungal susceptibility of 120 clinical C. parapsilosis isolates was examined. The changes in the gene expression related to resistance were analyzed. Results: Eight strains were resistant to fluconazole (FLC), itraconazole (ITC), and amphotericin B (AMB)...
February 8, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28160769/the-common-genetic-variant-of-insig2-gene-rs7566605-polymorphism-is-associated-with-severe-obesity-in-north-india
#9
Jai Prakash, Balraj Mittal, Srivastava Apurva, Awasthi Shally, Srivastava Pranjal, Srivastava Neena
Background: Obesity is a very common disorder resulting from an imbalance between food intake and energy expenditure, and it has a substantial impact on the development of chronic diseases. The aim of this study was to examine the association of insig2 (rs7566605) gene polymorphism with obesity and obesity associated phenotypes in North Indian subjects. Methods: The variants were investigated for association in 642 obese and non-obese individuals. The genotyping of insig2 (rs7566605) single nucleotide polymorphism was analyzed by the TaqMan allelic discrimination protocol...
February 5, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28131109/fabrication-of-chitosan-silk-based-tracheal-scaffold-using-freeze-casting-method
#10
Zeinab Nematollahi, Mohammad Tafazzoli-Shadpour, Ali Zamanian, Amir Seyedsalehi, Shadmehr Mohammad-Behgam, Fariba Ghorbani, Fereshte Mirahmadi
Background: Since the treatments of long tracheal leisions are associated with some limitations, tissue enginered trachea is considered as an alternative option. This study aimed at preparing a composite scaffold, based on natural and synthetic materials for tracheal tissue engineering. Methods: Nine chitosan silk-based scaffolds were fabricated using three freezing rates (0.5, 1, and 2°C/min) and glutaraldehyde (GA) concentrations (0, 0.4, and 0.8 wt%). Samples were characterized, and scaffolds having mechanical properties compatible with those of human trachea and proper biodegradability were selected for chondrocyte cell seeding and subsequent biological assessments...
January 29, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28088130/vaccine-candidates-against-nontypeable-haemophilus-influenzae-a-review
#11
REVIEW
Ava Behrouzi, Farzam Vaziri, Fatemeh Rahimi-Jamnani, Parviz Afrough, Mohammad Rahbar, Fereshteh Satarian, Seyed Davar Siadat
Nonencapsulated, nontypeable Hemophilus influenzae (NTHi) remains an important cause of acute otitis and respiratory diseases in children and adults. NTHi bacteria are one of the major causes of respiratory tract infections, including acute otitis media, cystic fibrosis, and community-acquired pneumonia among children, especially in developing countries. The bacteria can also cause chronic diseases such as chronic bronchitis and chronic obstructive pulmonary disease in the lower respiratory tract of adults...
March 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28088132/expression-and-secretion-of-cyan-fluorescent-protein-cfp-in-b-subtilis-using-the-chitinase-promoter-from-bacillus-pumilus-sg2
#12
Abbas Shali, Garshasb Rigi, Majid Pornour, Gholamreza Ahmadian
Background: Improved cyan fluorescent protein (ICFP) is a monochromic, green fluorescent protein (GFP) derivative produced by Aequorea macrodactyla in a process similar to GFP. This protein has strong absorption spectra at wavelengths 426-446 nm. ICFP can be used in cell, organelle or intracellular protein labeling, investigating the protein-protein interactions as well as assessing the promoter activities. Methods: In our previous study, the promoters of two chitinases (ChiS and ChiL) from Bacillus pumilus SG2 were assessed in B...
January 15, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28042145/hippocampal-expression-of-connexin36-and-connexin43-during-epileptogenesis-in-pilocarpine-model-of-epilepsy
#13
Sahel Motaghi, Mohammad Sayyah, Vahab Babapour, Reza Mahdian
BACKGROUND: Gap junctions (GJs) provide direct intercellular communications that are formed by hexameric protein subunits, called connexin (Cx). The role of Cxs in epileptogenesis has not received sufficient attention. Hippocampus with critical function in epileptogenesis has a wide network of GJs. We examined the protein expression levels of hippocampal Cx36 (the prominent Cx present between GABAergic interneurons) and Cx43 (the main Cx expressed by astrocytes) during epileptogenesis in the pilocarpine model of epilepsy...
January 2, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28088131/number-crunching-towards-molecular-barcoding
#14
Soroush Sardari
Molecules are the basic and most important players of life. Molecules are composed of atoms, and each molecule has two main specifications or inheritances that are structures, and in biomedical applications, biological function. This dichotomy can be compared to living organisms or humans whom possess genotype and phenotype. Each of the two main inherited elements contains a variety of feature classes and sub-categories. Due to ease of use, the scientific community focuses on elaborating the structure, rather than the function...
2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28000798/identification-and-evaluation-of-novel-drug-targets-against-the-human-fungal-pathogen-aspergillus-fumigatus-with-elaboration-on-the-possible-role-of-rna-binding-protein
#15
Saeid Malekzadeh, Soroush Sardari, Parisa Azerang, Dorsa Khorasanizadeh, Solmaz Agha Amiri, Mohammad Azizi, Nazanin Mohajerani, Vahid Khalaj
BACKGROUND: Aspergillus fumigatus is an airborne opportunistic fungal pathogen that can cause fatal infections in immunocompromised patients. Although the current anti-fungal therapies are relatively efficient, some issues such as drug toxicity, drug interactions, and the emergence of drug-resistant fungi have promoted the intense research toward finding the novel drug targets. METHODS: In search of new antifungal drug targets, we have used a bioinformatics approach to identify novel drug targets...
2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/27941964/study-break-bacterial-cancer
#16
Marjan Mohammadi
Rarely, do we think of cancer as “bacterial”, such that we are often told that “cancer is NOT contagious” Persistent bacterial infections cause persistent irritation of the host’s defense systems, which when ineffective in eradication of the infection, result in a multitude of self-destructive damages. This in some cases occurs to such severity that the resulting cellular hyper-proliferation provides grounds for increased chances of writing errors (gene mutations) and decreased chances of corrections (gene repair)...
January 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/27805072/evaluation-of-an-albumin-binding-domain-protein-fused-to-recombinant-human-il-2-and-its-effects-on-the-bioactivity-and-serum-half-life-of-the-cytokine
#17
Elham Adabi, Fateme Saebi, Amin Moradi Hasan-Abad, Ladan Teimoori-Toolabi, Gholam Ali Kardar
BACKGROUND: Background: Cancer immunotherapy is a promising strategy for cancer treatment. In this strategy, the immune system is triggered to destroy cancer cells. IL-2 is an important factor in passive cancer immunotherapy that helps modulating some important immune functions. One of the IL-2 limitations is low serum half-life; therefore, repetitive high doses of the injections are required to maintain effective concentrations. High-dose IL-2 therapy results in severe side effects; thus, improvement of its serum half-life would provide therapeutic benefits...
2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/27703278/ghrelin-exerts-analgesic-effects-through-modulation-of-il-10-and-tgf-%C3%AE-levels-in-a-rat-model-of-inflammatory-pain
#18
Faranak Azizzadeh, Javad Mahmoodi, Saeed Sadigh-Eteghad, Fereshteh Farajdokht, Gisou Mohaddes
BACKGROUND: Ghrelin is a peptide with attenuating effect on inflammatory pain. Both anti- and pro-inflammatory mediators have a role in the nociception and development of pain and hyperalgesia. IL-10 and TGF-β are anti-inflammatory cytokines and inhibit the expression of pro-inflammatory cytokines related to peripheral and central inflammatory pain. In this study, the effects of i.p. injection of ghrelin on the early and the late phases of pain, as well as serum levels of IL-10 and TGF-β, as anti-inflammatory cytokines, were investigated in formalin-induced pain in male rats...
2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/27695030/phosphorylation-of-staphylococcus-aureus-protein-tyrosine-kinase-affects-the-function-of-glucokinase-and-biofilm-formation
#19
Dudipeta Vasu, Pasupuleti Santhosh Kumar, Uppu Venkateswara Prasad, Vimjam Swarupa, Sthanikam Yeswanth, Lokanathan Srikanth, Manne Mudhu Sunitha, Abhijith Choudhary, Potukuchi Venkata Gurunadha Krishna Sarma
BACKGROUND: When Staphylococcus aureus is grown in the presence of high concentration of external glucose, this sugar is phosphorylated by glucokinase (glkA) to form glucose-6-phosphate. This product subsequently enters into anabolic phase, which favors biofilm formation. The presence of ROK (repressor protein, open reading frame, sugar kinase) motif, phosphate-1 and -2 sites, and tyrosine kinase sites in glkA of S. aureus indicates that phosphorylation must regulate the glkA activity...
2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/27664137/the-role-of-long-non-coding-rnas-in-ovarian-cancer
#20
REVIEW
Elahe Nikpayam, Behnoosh Tasharrofi, Shaghayegh Sarrafzadeh, Soudeh Ghafouri-Fard
Ovarian cancer is the most fatal tumor of female's reproductive system, and several genetics and environmental factors are involved in its development. Various studies have already identified some suitable biomarkers to facilitate the early detection, the prognosis evaluation, and the assessment of treatment response. However, the aim of this review is to investigate the role of long non-coding RNAs (lncRNAs) in tumorigenesis process of ovarian cancer and their potential applications as ovarian cancer biomarkers...
January 2017: Iranian Biomedical Journal
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