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Angiogenesis

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https://www.readbyqxmd.com/read/30003436/angiogenic-capacity-in-pre-eclampsia-and-uncomplicated-pregnancy-estimated-by-assay-of-angiogenic-proteins-and-an-in-vitro-vasculogenesis-angiogenesis-test
#1
Anita Virtanen, Outi Huttala, Kati Tihtonen, Tarja Toimela, Tuula Heinonen, Jukka Uotila
OBJECTIVE: The purpose of the study was to determine the angiogenic capacity of sera in early and late pregnancy and in umbilical blood serum after childbirth, and to define how angiogenic properties assessed in a functional in vitro test are related to individual angiogenic proteins in six women with pre-eclampsia and in six healthy pregnant controls. METHODS: Maternal first and third trimester serum samples, and umbilical blood samples after childbirth, were tested in an in vitro human adipose stromal cell-human umbilical vein endothelial cell (hASC-HUVEC) vasculogenesis/angiogenesis assay...
July 12, 2018: Angiogenesis
https://www.readbyqxmd.com/read/29987450/ephb4-mediates-resistance-to-antiangiogenic-therapy-in-experimental-glioma
#2
Christian Uhl, Moritz Markel, Thomas Broggini, Melina Nieminen, Irina Kremenetskaia, Peter Vajkoczy, Marcus Czabanka
INTRODUCTION: Alterations in vascular morphogenesis are hallmarks of antiangiogenesis-resistant tumor vessels. Vascular morphogenesis is regulated by ephrinB2-EphB4 system which may induce different biological effects depending on the oncological and molecular contexts. It was the aim of the current study to characterize the influence of EphB4 on tumor microcirculation after antiangiogenic treatment using different SF126 glioma models. MATERIALS AND METHODS: Using an ecotropic transfection system, empty vector (pLXSN) or EphB4 (EphB4OE ) overexpressing Phoenix-ECO cells were coimplanted with SF126 glioma cells subcutaneously (dorsal skinfold chamber, DSC) and orthotopically (cranial window, CW)...
July 10, 2018: Angiogenesis
https://www.readbyqxmd.com/read/29987448/inhibition-of-macrophage-inflammatory-protein-1%C3%AE-improves-endothelial-progenitor-cell-function-and-ischemia-induced-angiogenesis-in-diabetes
#3
Ting-Ting Chang, Liang-Yu Lin, Jaw-Wen Chen
Systemic inflammation might contribute to the impairment of neovasculogenesis and endothelial progenitor cell (EPC) function in clinical diabetes mellitus (DM). Macrophage inflammatory protein-1β (MIP-1β) is an inflammatory chemokine that may be up-regulated in clinical DM. Its role in diabetic vasculopathy was not clarified. This study aimed to investigate the role of MIP-1β in human EPCs and in neovasculogenesis in different diabetic animal models with hindlimb ischemia. EPCs chamber assay and in vitro tube formation assay were used to estimate the degree of EPC migration and tube formation abilities...
July 9, 2018: Angiogenesis
https://www.readbyqxmd.com/read/29971641/fluorescent-reporter-transgenic-mice-for-in-vivo-live-imaging-of-angiogenesis-and-lymphangiogenesis
#4
REVIEW
Susan J Doh, Michael Yamakawa, Samuel M Santosa, Mario Montana, Kai Guo, Joseph R Sauer, Nicholas Curran, Kyu-Yeon Han, Charles Yu, Masatsugu Ema, Mark I Rosenblatt, Jin-Hong Chang, Dimitri T Azar
The study of lymphangiogenesis is an emerging science that has revealed the lymphatic system as a central player in many pathological conditions including cancer metastasis, lymphedema, and organ graft rejection. A thorough understanding of the mechanisms of lymphatic growth will play a key role in the development of therapeutic strategies against these conditions. Despite the known potential of this field, the study of lymphatics has historically lagged behind that of hemangiogenesis. Until recently, significant strides in lymphatic studies were impeded by a lack of lymphatic-specific markers and suitable experimental models compared to those of the more immediately visible blood vasculature...
July 3, 2018: Angiogenesis
https://www.readbyqxmd.com/read/29967964/human-microvasculature-on-a-chip-anti-neovasculogenic-effect-of-nintedanib-in-vitro
#5
Soheila Zeinali, Colette A Bichsel, Nina Hobi, Manuela Funke, Thomas M Marti, Ralph A Schmid, Olivier T Guenat, Thomas Geiser
Idiopathic pulmonary fibrosis is characterized by a progressive scarring and stiffening of the peripheral lung tissue that decreases lung function. Over the course of the disease, the lung microvasculature undergoes extensive remodeling. There is increased angiogenesis around fibrotic foci and an absence of microvessels within the foci. To elucidate how the anti-fibrotic drug nintedanib acts on vascular remodeling, we used an in vitro model of perfusable microvessels made with primary endothelial cells and primary lung fibroblasts in a microfluidic chip...
July 2, 2018: Angiogenesis
https://www.readbyqxmd.com/read/29959560/mir-153-inhibits-the-migration-and-the-tube-formation-of-endothelial-cells-by-blocking-the-paracrine-of-angiopoietin-1-in-breast-cancer-cells
#6
Huichun Liang, Fei Ge, Yuhui Xu, Ji Xiao, Zhongmei Zhou, Rong Liu, Ceshi Chen
The sprouting of endothelial cells is the first step of tumor angiogenesis. Our previous study suggests that miR-153 suppresses breast tumor angiogenesis partially through targeting hypoxia-induced factor (HIF1α). In this study, we demonstrated that miR-153 also suppresses the migration and the tube formation of endothelial cells through directly targeting angiopoietin 1 (ANG1) in breast cancer cells. There was a negative correlation between miR-153 and ANG1 levels in breast cancer. miR-153 blocked the expression and secretion of ANG1 in breast cancer cells through binding to ANG1 mRNA...
June 29, 2018: Angiogenesis
https://www.readbyqxmd.com/read/29956018/regulation-of-angiogenesis-by-micrornas-in-cardiovascular-diseases
#7
REVIEW
Devika Kir, Erica Schnettler, Shrey Modi, Sundaram Ramakrishnan
Non-coding RNAs are functional RNA molecules comprising the majority of human transcriptome. Only about 1.5% of the human genome is transcribed into messenger RNAs (mRNA) that are translated into proteins. Among the non-coding RNAs, miRNAs are extensively studied and miR targets in endothelial cells, perivascular cells, and angiogenic signaling are relatively well defined. MicroRNAs not only regulate transcripts in situ but also function as paracrine mediators in affecting angiogenesis at distant sites. Exosomal miRs are implicated in modulating endothelial cell function and angiogenesis...
June 28, 2018: Angiogenesis
https://www.readbyqxmd.com/read/29956017/heparin-impairs-angiogenic-signaling-and-compensatory-lung-growth-after-left-pneumonectomy
#8
Duy T Dao, Lorenzo Anez-Bustillos, Jared Ourieff, Amy Pan, Paul D Mitchell, Hiroko Kishikawa, Gillian L Fell, Meredith A Baker, Randolph S Watnick, Hong Chen, Thomas E Hamilton, Michael S Rogers, Diane R Bielenberg, Mark Puder
Children with hypoplastic lung diseases, such as congenital diaphragmatic hernia, can require life support via extracorporeal membrane oxygenation and systemic anticoagulation, usually in the form of heparin. The role of heparin in angiogenesis and organ growth is inconclusive, with conflicting data reported in the literature. This study aimed to investigate the effects of heparin on lung growth in a model of compensatory lung growth (CLG). Compared to the absence of heparin, treatment with heparin decreased the vascular endothelial growth factor (VEGF)-mediated activation of VEGFR2 and mitogenic effect on human lung microvascular endothelial cells in vitro...
June 28, 2018: Angiogenesis
https://www.readbyqxmd.com/read/29951828/igf2-and-igf1r-identified-as-novel-tip-cell-genes-in-primary-microvascular-endothelial-cell-monolayers
#9
Marchien G Dallinga, Bahar Yetkin-Arik, Richelle P Kayser, Ilse M C Vogels, Patrycja Nowak-Sliwinska, Arjan W Griffioen, Cornelis J F van Noorden, Ingeborg Klaassen, Reinier O Schlingemann
Tip cells, the leading cells of angiogenic sprouts, were identified in cultures of human umbilical vein endothelial cells (HUVECs) by using CD34 as a marker. Here, we show that tip cells are also present in primary human microvascular endothelial cells (hMVECs), a more relevant endothelial cell type for angiogenesis. By means of flow cytometry, immunocytochemistry, and qPCR, it is shown that endothelial cell cultures contain a dynamic population of CD34+ cells with many hallmarks of tip cells, including filopodia-like extensions, elevated mRNA levels of known tip cell genes, and responsiveness to stimulation with VEGF and inhibition by DLL4...
June 27, 2018: Angiogenesis
https://www.readbyqxmd.com/read/29943214/correction-to-gene-therapy-knockdown-of-vegfr2-in-retinal-endothelial-cells-to-treat-retinopathy
#10
Aaron B Simmons, Colin A Bretz, Haibo Wang, Eric Kunz, Kassem Hajj, Carson Kennedy, Zhihong Yang, Thipparat Suwanmanee, Tal Kafri, M Elizabeth Hartnett
The article "Gene therapy knockdown of VEGFR2 in retinal endothelial cells to treat retinopathy", written by "Aaron B. Simmons, Colin A. Bretz, Haibo Wang, Eric Kunz, Kassem Hajj, Carson Kennedy, Zhihong Yang, Thipparat Suwanmanee, Tal Kafri and M. Elizabeth Hartnett", was originally published electronically on the publisher's internet portal (currently SpringerLink) on 05 May 2018 without open access. With the author(s)' decision to opt for Open Choice the copyright of the article changed on 20 June 2018 to...
June 25, 2018: Angiogenesis
https://www.readbyqxmd.com/read/29846863/prion-protein-is-essential-for-diabetic-retinopathy-associated-neovascularization
#11
Lingyan Zhu, Jixiong Xu, Ying Liu, Tian Gong, Jianying Liu, Qiong Huang, Shane Fischbach, Wenquan Zou, Xiangwei Xiao
Diabetic retinopathy (DR), a major complication of diabetes caused by vascular damage and pathological proliferation of retinal vessels, often progresses to vision loss. Vascular endothelial growth factor (VEGF) signaling plays a pivotal role in the development of DR, but the exact underlying molecular mechanisms remain ill-defined. Cellular prion protein (PrPc ) is a surface protein expressed by vascular endothelial cells, and the increased expression of PrPc is associated with physiological and pathological vascularization...
May 30, 2018: Angiogenesis
https://www.readbyqxmd.com/read/29845518/endothelial-loss-of-fzd5-stimulates-pkc-ets1-mediated-transcription-of-angpt2-and-flt1
#12
Maarten M Brandt, Christian G M van Dijk, Ihsan Chrifi, Heleen M Kool, Petra E Bürgisser, Laura Louzao-Martinez, Jiayi Pei, Robbert J Rottier, Marianne C Verhaar, Dirk J Duncker, Caroline Cheng
AIMS: Formation of a functional vascular system is essential and its formation is a highly regulated process initiated during embryogenesis, which continues to play important roles throughout life in both health and disease. In previous studies, Fzd5 was shown to be critically involved in this process and here we investigated the molecular mechanism by which endothelial loss of this receptor attenuates angiogenesis. METHODS AND RESULTS: Using short interference RNA-mediated loss-of-function assays, the function and mechanism of signaling via Fzd5 was studied in human endothelial cells (ECs)...
May 29, 2018: Angiogenesis
https://www.readbyqxmd.com/read/29786783/a-xenograft-model-for-venous-malformation
#13
Jillian Goines, Xian Li, Yuqi Cai, Paula Mobberley-Schuman, Megan Metcalf, Steven J Fishman, Denise M Adams, Adrienne M Hammill, Elisa Boscolo
Vascular malformations are defects caused by the abnormal growth of the vasculature. Among them, venous malformation (VM) is an anomaly characterized by slow-flow vascular lesions with abnormally shaped veins, typically in sponge-like configuration. VMs can expand over years causing disfigurement, obstruction of vital structures, thrombosis, bleeding, and pain. Treatments have been very limited and primarily based on supportive care, compression garments, sclerotherapy, and/or surgical resection. Sirolimus treatment has recently shown efficacy in some patients with complicated vascular anomalies, including VMs...
May 21, 2018: Angiogenesis
https://www.readbyqxmd.com/read/29786782/preclinical-impact-of-high-dose-intermittent-antiangiogenic-tyrosine-kinase-inhibitor-pazopanib-in-intrinsically-resistant-tumor-models
#14
Elaine Reguera-Nuñez, Shan Man, Ping Xu, Robert S Kerbel
Antiangiogenic tyrosine kinase inhibitors (TKIs) target vascular endothelial growth factor receptors and other receptor tyrosine kinases. As a result of toxicity, the clinical failures or the modest benefits associated with antiangiogenic TKI therapy may be related in some cases to suboptimal drug dosing and scheduling, thereby facilitating resistance. Most antiangiogenic TKIs, including pazopanib, are administered on a continuous daily basis. Here, instead, we evaluated the impact of increasing the dose and administering the drug intermittently...
May 21, 2018: Angiogenesis
https://www.readbyqxmd.com/read/29777314/improved-recovery-from-limb-ischaemia-by-delivery-of-an-affinity-isolated-heparan-sulphate
#15
Selina Poon, Xiaohua Lu, Raymond A A Smith, Pei Ho, Kishore Bhakoo, Victor Nurcombe, Simon M Cool
Peripheral arterial disease is a major cause of limb loss and its prevalence is increasing worldwide. As most standard-of-care therapies yield only unsatisfactory outcomes, more options are needed. Recent cell- and molecular-based therapies that have aimed to modulate vascular endothelial growth factor-165 (VEGF165 ) levels have not yet been approved for clinical use due to their uncertain side effects. We have previously reported a heparan sulphate (termed HS7) tuned to avidly bind VEGF165 . Here, we investigated the ability of HS7 to promote vascular recovery in a murine hindlimb vascular ischaemia model...
May 18, 2018: Angiogenesis
https://www.readbyqxmd.com/read/29766399/consensus-guidelines-for-the-use-and-interpretation-of-angiogenesis-assays
#16
REVIEW
Patrycja Nowak-Sliwinska, Kari Alitalo, Elizabeth Allen, Andrey Anisimov, Alfred C Aplin, Robert Auerbach, Hellmut G Augustin, David O Bates, Judy R van Beijnum, R Hugh F Bender, Gabriele Bergers, Andreas Bikfalvi, Joyce Bischoff, Barbara C Böck, Peter C Brooks, Federico Bussolino, Bertan Cakir, Peter Carmeliet, Daniel Castranova, Anca M Cimpean, Ondine Cleaver, George Coukos, George E Davis, Michele De Palma, Anna Dimberg, Ruud P M Dings, Valentin Djonov, Andrew C Dudley, Neil P Dufton, Sarah-Maria Fendt, Napoleone Ferrara, Marcus Fruttiger, Dai Fukumura, Bart Ghesquière, Yan Gong, Robert J Griffin, Adrian L Harris, Christopher C W Hughes, Nan W Hultgren, M Luisa Iruela-Arispe, Melita Irving, Rakesh K Jain, Raghu Kalluri, Joanna Kalucka, Robert S Kerbel, Jan Kitajewski, Ingeborg Klaassen, Hynda K Kleinmann, Pieter Koolwijk, Elisabeth Kuczynski, Brenda R Kwak, Koen Marien, Juan M Melero-Martin, Lance L Munn, Roberto F Nicosia, Agnes Noel, Jussi Nurro, Anna-Karin Olsson, Tatiana V Petrova, Kristian Pietras, Roberto Pili, Jeffrey W Pollard, Mark J Post, Paul H A Quax, Gabriel A Rabinovich, Marius Raica, Anna M Randi, Domenico Ribatti, Curzio Ruegg, Reinier O Schlingemann, Stefan Schulte-Merker, Lois E H Smith, Jonathan W Song, Steven A Stacker, Jimmy Stalin, Amber N Stratman, Maureen Van de Velde, Victor W M van Hinsbergh, Peter B Vermeulen, Johannes Waltenberger, Brant M Weinstein, Hong Xin, Bahar Yetkin-Arik, Seppo Yla-Herttuala, Mervin C Yoder, Arjan W Griffioen
The formation of new blood vessels, or angiogenesis, is a complex process that plays important roles in growth and development, tissue and organ regeneration, as well as numerous pathological conditions. Angiogenesis undergoes multiple discrete steps that can be individually evaluated and quantified by a large number of bioassays. These independent assessments hold advantages but also have limitations. This article describes in vivo, ex vivo, and in vitro bioassays that are available for the evaluation of angiogenesis and highlights critical aspects that are relevant for their execution and proper interpretation...
May 15, 2018: Angiogenesis
https://www.readbyqxmd.com/read/29761249/targeting-glioblastoma-derived-pericytes-improves-chemotherapeutic-outcome
#17
REVIEW
Daniel A P Guerra, Ana E Paiva, Isadora F G Sena, Patrick O Azevedo, Walison N Silva, Akiva Mintz, Alexander Birbrair
Glioblastoma is the most common malignant brain cancer in adults, with poor prognosis. The blood-brain barrier limits the arrival of several promising anti-glioblastoma drugs, and restricts the design of efficient therapies. Recently, by using state-of-the-art technologies, including thymidine kinase targeting system in combination with glioblastoma xenograft mouse models, it was revealed that targeting glioblastoma-derived pericytes improves chemotherapy efficiency. Strikingly, ibrutinib treatment enhances chemotherapeutic effectiveness, by targeting pericytes, improving blood-brain barrier permeability, and prolonging survival...
May 14, 2018: Angiogenesis
https://www.readbyqxmd.com/read/29748783/correction-to-soluble-delta-like-1-homolog-dlk1-stimulates-angiogenesis-through-notch1-akt-enos-signaling-in-endothelial-cells
#18
Chao-Cheng Huang, Hsiao-Mei Kuo, Pei-Chang Wu, Shih-Hsuan Cheng, Tzu-Ting Chang, Yi-Chen Chang, Mei-Lang Kung, Deng-Chyang Wu, Jiin-Haur Chuang, Ming-Hong Tai
In the original publication of the article, there is an error in one of the citations in the Discussion section.
May 10, 2018: Angiogenesis
https://www.readbyqxmd.com/read/29737439/enos-expression-and-no-release-during-hypoxia-is-inhibited-by-mir-200b-in-human-endothelial-cells
#19
Anna Janaszak-Jasiecka, Anna Siekierzycka, Sylwia Bartoszewska, Marcin Serocki, Lawrence W Dobrucki, James F Collawn, Leszek Kalinowski, Rafal Bartoszewski
The nitric oxide (NO) secreted by vascular endothelium is required for the maintenance of cardiovascular homeostasis. Diminished release of NO generated by endothelial NO synthase contributes to endothelial dysfunction. Hypoxia and ischemia reduce endothelial eNOS expression via posttranscriptional mechanisms that result in NOS3 transcript destabilization. Here, we examine whether microRNAs contribute to this mechanism. We followed the kinetics of hypoxia-induced changes in NOS3 mRNA and eNOS protein levels in primary human umbilical vein endothelial cells (HUVECs)...
May 8, 2018: Angiogenesis
https://www.readbyqxmd.com/read/29730824/gene-therapy-knockdown-of-vegfr2-in-retinal-endothelial-cells-to-treat-retinopathy
#20
Aaron B Simmons, Colin A Bretz, Haibo Wang, Eric Kunz, Kassem Hajj, Carson Kennedy, Zhihong Yang, Thipparat Suwanmanee, Tal Kafri, M Elizabeth Hartnett
Inhibition of vascular endothelial growth factor (VEGF) in retinopathy of prematurity (ROP) raises concerns for premature infants because VEGF is essential for retinovascular development as well as neuronal and glial health. This study tested the hypothesis that endothelial cell-specific knockdown of VEGF receptor 2 (VEGFR2), or downstream STAT3, would inhibit VEGF-induced retinopathy without delaying physiologic retinal vascular development. We developed an endothelial cell-specific lentiviral vector that delivered shRNAs to VEGFR2 or STAT3 and a green fluorescent protein reporter under control of the VE-cadherin promoter...
May 5, 2018: Angiogenesis
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