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Pharmaceutical Biology

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https://www.readbyqxmd.com/read/28738722/effects-of-indirubin-and-isatin-on-cell-viability-mutagenicity-genotoxicity-and-bax-ercc1-gene-expression
#1
Manoela Viar Fogaça, Priscila de Matos Cândido-Bacani, Lucas Milanez Benicio, Lara Martinelli Zapata, Priscilla de Freitas Cardoso, Marcelo Tempesta de Oliveira, Tamara Regina Calvo, Eliana Aparecida Varanda, Wagner Vilegas, Ilce Mara de Syllos Cólus
CONTEXT: Indigofera suffruticosa Miller (Fabaceae) and I. truxillensis Kunth produce compounds, such as isatin (ISA) and indirubin (IRN), which possess antitumour properties. Their effects in mammalian cells are still not very well understood. OBJECTIVE: We evaluated the activities of ISA and/or IRN on cell viability and apoptosis in vitro, their genotoxic potentials in vitro and in vivo, and the IRN- and ISA-induced expression of ERCC1 or BAX genes. MATERIALS AND METHODS: HeLa and/or CHO-K1 cell lines were tested (3 or 24 h) in the MTT, Trypan blue exclusion, acridine orange/ethidium bromide, cytokinesis-blocked micronucleus (CBMN) and comet (36, 24 and 72 h) tests after treatment with IRN (0...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28738717/optimization-of-simultaneous-microwave-ultrasonic-assisted-extraction-of-phenolic-compounds-from-walnut-flour-using-response-surface-methodology
#2
Yan Luo, Wanxing Wu, Dan Chen, Yuping Lin, Yage Ma, Chaoyin Chen, Shenglan Zhao
CONTEXT: Walnut is a traditional food as well as a traditional medicine recorded in the Chinese Pharmacopoeia; however, the large amounts of walnut flour (WF) generated in walnut oil production have not been well utilized. OBJECTIVE: This study maximized the total polyphenolic yield (TPY) from the walnut flour (WF) by optimizing simultaneous ultrasound/microwave-assisted hydroalcoholic extraction (SUMAE). MATERIALS AND METHODS: Response surface methodology was used to optimize the processing parameters for the TPY, including microwave power (20-140 W), ultrasonic power (75-525 W), extraction temperature (25-55 °C), and time (0...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28738715/antimicrobial-modulatory-and-chemical-analysis-of-the-oil-of-croton-limae
#3
Tiago Rodrigues Leite, Maria Arlene Pessoa da Silva, Antônio Carlito Bezerra Dos Santos, Henrique Douglas Melo Coutinho, Antonia Eliene Duarte, José Galberto Martins da Costa
CONTEXT: Croton sp. are plants with a well-reported antimicrobial activity. Croton limae A.P. Gomes, M.F. Sales P.E. Berry (Euphorbiaceae), known as 'marmeleiro-prateado', is commonly used to manage abdominal pain in Brazil. OBJECTIVE: This work evaluates the phytochemical composition, antimicrobial and modulatory activities of the essential oil of C. limae leaves (EOCL). MATERIALS AND METHODS: The minimum inhibitory concentration (MIC) and the modulation of the antibiotic activity were determined using a microdilution method...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28738710/use-of-traditional-herbal-medicine-as-an-alternative-in-dental-treatment-in-mexican-dentistry-a-review
#4
Cindy Cruz Martínez, Martha Diaz Gómez, Myung Sook Oh
CONTEXT: Herbal therapies are used worldwide to treat health conditions. In Mexico, generations have used them to treat gingivitis, periodontitis, mouth infections, and discoloured teeth. However, few studies have collected scientific evidence on their effects. OBJECTIVE: This study aimed at searching and compiling scientific evidence of alternative oral and dental treatments using medicinal herbs from Mexico. METHODS: We collected various Mexican medicinal plants used in the dental treatment from the database of the Institute of Biology at the National Autonomous University of Mexico...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28728492/the-bioactivity-of-hedysarum-coronarium-extracts-on-skin-enzymes-and-cells-correlates-with-phenolic-content
#5
Bruno Burlando, Giulia Pastorino, Annalisa Salis, Gianluca Damonte, Marco Clericuzio, Laura Cornara
CONTEXT: The search for bioactive compounds from botanical sources is attracting much interest. However, differences in chemical composition may occur within the same species depending on different geographical origins. OBJECTIVES: We evaluated the properties on skin enzymes and cells of extracts from sulla legume crop Hedysarum coronarium L. (Fabaceae), collected at two Italian sites near Pisa and Ventimiglia, for possible dermatological and cosmetic applications...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28728456/quillaja-saponaria-bark-saponin-protects-wistar-rats-against-ferrous-sulphate-induced-oxidative-and-inflammatory-liver-damage
#6
Mustafa Ahmed Abdel-Reheim, Basim Anwar Shehata Messiha, Ali Ahmed Abo-Saif
CONTEXT: Saponins from different sources are historically reported in Chinese medicine to possess many beneficial effects. However, insufficient experimental data are available regarding the hepatoprotective potential of Quillaja bark saponin. OBJECTIVE: The protective effect of Quillaja saponaria Molina (Quillajaceae) bark triterpenoid saponin against iron-induced hepatotoxicity is compared to the standard N-acetylcysteine in adult male Wistar rats. MATERIALS AND METHODS: Animals were divided into (six) groups, namely a normal control, an N-acetylcysteine control (300 mg/kg/day, p...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28726567/possible-mechanisms-involved-in-the-anti-nociceptive-effects-of-hydro-ethanolic-leaf-extract-of-ziziphus-abyssinica
#7
Eric Boakye-Gyasi, Isaac Tabiri Henneh, Wonder Kofi Mensah Abotsi, Elvis Ofori Ameyaw, Eric Woode
CONTEXT: Various parts of Ziziphus abyssinica Hochst ex. A. Rich (Rhamnaceae) have been used in Ghanaian and African traditional medicine as an analgesic. However, there are little scientific data to support the anti-nociceptive effects of the hydro-ethanolic leaf extract of Ziziphus abyssinica (EthE) as well as the possible mechanisms involved in its anti-nociceptive effects. PURPOSE: To predict possible nociceptive pathways involved in the anti-nociceptive effects of EthE...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28724331/randomized-double-blinded-pilot-clinical-study-of-the-antidiabetic-activity-of-balanites-aegyptiaca-and-uplc-esi-ms-ms-identification-of-its-metabolites
#8
Hend Rashad, Fateheya M Metwally, Shahira M Ezzat, Maha M Salama, Adel Hasheesh, Amira Abdel Motaal
CONTEXT: Balanites aegyptiaca Del. (Zygophyllaceae) fruits are traditionally known for the treatment of hyperglycaemia. Several in vitro and in vivo studies proposed some mechanisms of action. However, clinical trials in human beings were never reported to date. OBJECTIVES: To investigate the antidiabetic efficacy of the 70% ethanol extract of the pericarps of B. aegyptiaca (BE) within a nutritional intervention in elderly people. MATERIALS AND METHODS: Ultra-performance electrospray ionization-mass spectroscopy (UPLC-ESI-MS/MS) analysis was used for metabolic profiling of BE which was incorporated in hard gelatine capsules (400 mg/day) and tested on 30 type 2 diabetes (T2D) Egyptian patients for 8 weeks...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28724321/diospyros-kaki-calyx-inhibits-immediate-type-hypersensitivity-via-the-reduction-of-mast-cell-activation
#9
Min-Jong Kim, Hae Ran Park, Tae-Yong Shin, Sang-Hyun Kim
CONTEXT: Diospyros kaki L. (Ebenaceae) fruit is widely distributed in Asia and is known to exert anti-inflammatory and antithrombotic effects. OBJECTIVE: We evaluated the inhibitory effect of aqueous extract of D. kaki calyx (AEDKC) on mast cell-mediated immediate-type hypersensitivity and underlying mechanism of action. MATERIALS AND METHODS: For in vivo, ovalbumin (OVA)-induced active systemic anaphylaxis (ASA) and immunoglobulin (Ig) E-mediated passive cutaneous anaphylaxis (PCA) models were used...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28675957/protective-mechanism-of-turmeric-curcuma-longa-on-carbofuran-induced-hematological-and-hepatic-toxicities-in-a-rat-model
#10
Md Sakib Hossen, E M Tanvir, Maruf Billah Prince, Sudip Paul, Moumoni Saha, Md Yousuf Ali, Siew Hua Gan, Md Ibrahim Khalil, Nurul Karim
CONTEXT: Turmeric (Curcuma longa L. [Zingiberaceae]) is used in the treatment of a variety of conditions including pesticide-induced toxicity. OBJECTIVE: The study reports the antioxidant properties and the protective effects of turmeric against carbofuran (CF)-induced toxicity in rats. MATERIALS AND METHODS: The antioxidant potential was determined by using free radicals scavenging activity and ferric reducing antioxidant power values. Male Wistar rats were randomly divided into four groups, designated as control, turmeric (100 mg/kg/day), CF (1 mg/kg/day) and turmeric (100 mg/kg/day) + CF (1 mg/kg/day) treatments...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28659002/in-vitro-and-in-vivo-antidiabetic-potential-of-extracts-and-a-furostanol-saponin-from-balanites-aegyptiaca
#11
Shahira Mohammed Ezzat, Amira Abdel Motaal, Sally Abdel Wanees El Awdan
CONTEXT: Balanites aegyptiaca Del. (Zygophyllaceae) fruits are well-known antidiabetic drug in Egyptian folk medicine. Nevertheless, its mechanism of action is still unclear. OBJECTIVES: Searching for the possible mechanisms of action of the plant and identification of its bioactive compounds. MATERIALS AND METHODS: A bio-guided protocol based on the evaluation of α-glucosidase (AG) and aldose reductase (AR) inhibitory activities was adopted to isolate the biologically active compounds from the methanol extract (MeEx)...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28659001/evaluation-of-the-cytotoxic-potential-of-a-new-pentacyclic-triterpene-from-rhododendron-arboreum-stem-bark
#12
Sajid Ali, Muhammad Nisar, Muhammad Qaisar, Aslam Khan, Abid Ali Khan
CONTEXT: Traditionally, Rhododendron arboreum Sm. (Ericaceae) is a very important medicinal plant having oxytocic, estrogenic, anti-inflammatory, analgesic and hepatoprotective activities; it also inhibits the prostaglandin synthetase. OBJECTIVES: This study determines the cytotoxic potential of 15-oxoursolic acid isolated from R. arboreum against selected human cancer cell lines. MATERIALS AND METHODS: Extraction from stem bark (5 kg) of R...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28651482/modulation-of-some-markers-of-erectile-dysfunction-and-malonaldehyde-levels-in-isolated-rat-penile-tissue-with-unripe-and-ripe-plantain-peels-identification-of-the-constituents-of-the-plants-using-hplc
#13
Ganiyu Oboh, Adedayo Oluwaseun Ademiluyi, Sunday Idowu Oyeleye, Tosin Abiola Olasehinde, Aline Augusti Boligon
CONTEXT: Plantain fruit pulp has been used as a natural remedy to manage erectile dysfunction (ED) in traditional medicine. However, the potency of the peel has not been examined with respect to ED management. OBJECTIVE: This study investigated and compared the inhibitory potential of unripe (UPP) and ripe (RPP) plantain peels on some enzymes associated with ED and Fe(2+)-induced oxidative stress in albino rat penile homogenate in vitro. MATERIALS AND METHOD: Aqueous extract of the peels was prepared and the effect on phosphodiesterase-5 (PDE-5), arginase, acetylcholinesterase (AChE), angiotensin-I converting enzyme (ACE) and Fe(2+)-induced malonyladehyde in isolated albino rat penile homogenate were investigated...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28651481/anti-fibrotic-effects-of-cuscuta-chinensis-with-in-vitro-hepatic-stellate-cells-and-a-thioacetamide-induced-experimental-rat-model
#14
Jin Seoub Kim, Sushruta Koppula, Mun Jeong Yum, Gwang Mo Shin, Yun Jin Chae, Seok Min Hong, Jae Dong Lee, MinDong Song
CONTEXT: Cuscuta chinensis Lam. (Convolvulaceae) has been used as a traditional herbal remedy for treating liver and kidney disorders. OBJECTIVE: Anti-fibrotic effects of C. chinensis extract (CCE) in cellular and experimental animal models were investigated. MATERIALS AND METHODS: HSC-T6 cell viability, cell cycle and apoptosis were analysed using MTT assay, flow cytometry and Annexin V-FITC/PI staining techniques. Thioacetamide (TAA)-induced fibrosis model was established using Sprague Dawley rats (n = 10)...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28644062/4-nerolidylcatechol-apoptosis-by-mitochondrial-mechanisms-with-reduction-in-cyclin-d1-at-g0-g1-stage-of-the-chronic-myelogenous-k562-cell-line
#15
Polyana Lopes Benfica, Renato Ivan de Ávila, Bruna Dos Santos Rodrigues, Alane Pereira Cortez, Aline Carvalho Batista, Marilisa Pedroso Nogueira Gaeti, Eliana Martins Lima, Kênnia Rocha Rezende, Marize Campos Valadares
CONTEXT: 4-Nerolidylcatechol (4-NRC) has showed antitumor potential through apoptosis. However, its apoptotic mechanisms are still unclear, especially in leukemic cells. OBJECTIVES: To evaluate the cytotoxic potential of 4-NRC and its cell death pathways in p53-null K562 leukemic cells. MATERIALS AND METHODS: Cytotoxicity of 4-NRC (4.17-534.5 μM) over 24 h of exposure was evaluated by MTT assay. 4-NRC-induced apoptosis in K562 cells was investigated by phosphatidylserine (PS) externalization, cell cycle, sub-G1, mitochondrial evaluation, cytochrome c, cyclin D1 and intracellular reactive oxygen species (ROS) levels, and caspase activity analysis...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28633584/variation-of-alkaloid-contents-and-antimicrobial-activities-of-papaver-rhoeas-l-growing-in-turkey-and-northern-cyprus
#16
İlkcan Çoban, Gizem Gülsoy Toplan, Berna Özbek, Çağlayan Unsal Gürer, Günay Sarıyar
CONTEXT: Papaver rhoeas L. (Papaveraceae) corn poppy, widely distributed in Turkey, is used to make a cough syrup for children, as a tea for disturbed sleep, for pain relief and as a sedative in folk medicine. OBJECTIVE: Samples of P. rhoeas collected from eight different locations in Turkey and three from northern Cyprus were investigated for their alkaloid content and screened for their antimicrobial activities. MATERIALS AND METHODS: From the aerial parts of P...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28631525/polyphenol-profile-by-uhplc-ms-ms-anti-glycation-antioxidant-and-cytotoxic-activities-of-several-samples-of-propolis-from-the-northeastern-semi-arid-region-of-brazil
#17
Jadriane de Almeida Xavier, Iara Barros Valentim, Fabiana O S Camatari, Alberto M M de Almeida, Henrique Fonseca Goulart, Jamylle Nunes de Souza Ferro, Emiliano de Oliveira Barreto, Bruno Coelho Cavalcanti, Carla B G Bottoli, Marília Oliveira Fonseca Goulart
CONTEXT: Propolis has promising biological activities. Propolis samples from the Northeast of Bahia, Brazil - sample A from Ribeira do Pombal and B, from Tucano - were investigated, with new information regarding their biological activities. OBJECTIVE: This paper describes the chemical profile, antioxidant, anti-glycation and cytotoxic activities of these propolis samples. MATERIAL AND METHODS: Ethanol extracts of these propolis samples (EEP) and their fractions were analyzed to determine total phenolic content (TPC); antioxidant capacity through DPPH(•), FRAP and lipid peroxidation; anti-glycation activity, by an in vitro glucose (10 mg/mL) bovine serum albumine (1 mg/mL) assay, during 7 d; cytotoxic activity on cancer (SF295, HCT-116, OVCAR-8, MDA-MB435, MX-1, MCF7, HL60, JURKAT, MOLT-4, K562, PC3, DU145) and normal cell lines (V79) at 0...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28629287/anti-acetylcholinesterase-activity-and-antioxidant-properties-of-extracts-and-fractions-of-carpolobia-lutea
#18
Lucky Legbosi Nwidu, Ekramy Elmorsy, Jack Thornton, Buddhika Wijamunige, Anusha Wijesekara, Rebecca Tarbox, Averil Warren, Wayne Grant Carter
CONTEXT: There is an unmet need to discover new treatments for Alzheimer's disease. This study determined the anti-acetylcholinesterase (AChE) activity, DPPH free radical scavenging and antioxidant properties of Carpolobia lutea G. Don (Polygalaceae). OBJECTIVE: The objective of this study is to quantify C. lutea anti-AChE, DPPH free radical scavenging, and antioxidant activities and cell cytotoxicity. MATERIALS AND METHODS: Plant stem, leaves and roots were subjected to sequential solvent extractions, and screened for anti-AChE activity across a concentration range of 0...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28614988/in-vitro-inhibitory-effects-of-dihydromyricetin-on-human-liver-cytochrome-p450-enzymes
#19
Lu Liu, Sen Sun, Hongbing Rui, Xiaohua Li
CONTEXT: Dihydromyricetin (DHM) is the most abundant and active flavonoid component isolated from Ampelopsis grossedentata (Hand-Mazz) W.T. Wang (Vitaceae) and it possesses numerous pharmacological activities. However, whether DHM affects the activity of human liver cytochrome P450 (CYP) enzymes remains unclear. MATERIALS AND METHODS: The inhibitory effects of DHM on eight human liver CYP isoforms (i.e., 1A2, 3A4, 2A6, 2E1, 2D6, 2C9, 2C19 and 2C8) were investigated in vitro using human liver microsomes (HLMs)...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28614972/anti-inflammatory-effects-of-a-traditional-korean-medicine-ojayeonjonghwan
#20
Sun-Young Nam, Kyu-Yeob Kim, Mi Hye Kim, Jae-Bum Jang, So-Young Rah, Hee Jeong Chae, Jin-Man Lee, Hyung-Min Kim, Hyun-Ja Jeong
OBJECTIVE: To study the anti-inflammatory properties of OJ. CONTEXT: Ojayeonjonghwan (OJ) is a traditional Korean prescription, which has been widely used for the treatment of prostatitis. However, no scientific study has been performed of the anti-inflammatory effects of OJ. MATERIALS AND METHODS: Peritoneal macrophages were isolated 3-4 days after injecting a C57BL/6J mouse with thioglycollate. They were then treated with OJ water extract (0...
December 2017: Pharmaceutical Biology
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