journal
MENU ▼
Read by QxMD icon Read
search

Current Opinion in Chemical Biology

journal
https://www.readbyqxmd.com/read/28735229/synthetic-antibody-mimics-for-the-inhibition-of-protein-ligand-interactions
#1
REVIEW
Christina Haußner, Johannes Lach, Jutta Eichler
The rational/structure-based design and/or combinatorial development of molecules capable of selectively binding to a protein, represents a promising strategy for a range of biomedical applications, in particular the inhibition of disease-associated protein-ligand interactions. The design of such protein binding molecules is often based on an antibody against the target protein, or involves the generation of smaller molecules that retain the binding characteristics of the antibody. Alternatively, protein binding molecules can be selected from protein libraries based on small, stably folded protein scaffolds presenting flexible loops, which are randomized in the libraries...
July 20, 2017: Current Opinion in Chemical Biology
https://www.readbyqxmd.com/read/28735187/progress-in-targeting-the-bcl-2-family-of-proteins
#2
REVIEW
Thomas P Garner, Andrea Lopez, Denis E Reyna, Adam Z Spitz, Evripidis Gavathiotis
The network of protein-protein interactions among the BCL-2 protein family plays a critical role in regulating cellular commitment to mitochondrial apoptosis. Anti-apoptotic BCL-2 proteins are considered promising targets for drug discovery and exciting clinical progress has stimulated intense investigations in the broader family. Here, we discuss recent developments in small molecules targeting anti-apoptotic proteins and alternative approaches to targeting BCL-2 family interactions. These studies advance our understanding of the role of BCL-2 family proteins in physiology and disease, providing unique tools for dissecting these functions...
July 20, 2017: Current Opinion in Chemical Biology
https://www.readbyqxmd.com/read/28732278/advances-of-small-molecule-targeting-of-kinases
#3
REVIEW
Norbert Berndt, Rezaul M Karim, Ernst Schönbrunn
Reversible protein phosphorylation regulates virtually all aspects of life in the cell. As a result, dysregulation of protein kinases, the enzymes responsible for transferring phosphate groups from ATP to proteins, are often the cause or consequence of many human diseases including cancer. Almost three dozen protein kinase inhibitors (PKIs) have been approved for clinical applications since 1995, the vast majority of them for the treatment of cancer. According to the NCI, there are more than 100 types of cancer...
July 18, 2017: Current Opinion in Chemical Biology
https://www.readbyqxmd.com/read/28709120/advances-in-design-of-protein-folds-and-assemblies
#4
REVIEW
Ajasja Ljubetič, Helena Gradišar, Roman Jerala
Conceptual and computational advances triggered an explosion of designed protein structures in the recent years. Various protein fold geometries have been robustly designed with atomic accuracy, including protein folds unseen in nature. The same principles and tools have been extended to design multi-chain assemblies. By exploiting symmetry, mega-Dalton structures have been created with exciting potential applications for synthetic biology. In this review we focus on design of single chain and multi polypeptide chain assemblies of defined size and composition...
July 11, 2017: Current Opinion in Chemical Biology
https://www.readbyqxmd.com/read/28689146/chemical-modulators-for-epigenome-reader-domains-as-emerging-epigenetic-therapies-for-cancer-and-inflammation
#5
REVIEW
Nilesh Zaware, Ming-Ming Zhou
Site-specific lysine acetylation and methylation on histones are critical post-translational modifications (PTMs) that govern ordered gene transcription in chromatin. Mis-regulation of these histone PTM-mediated processes has been shown to be associated with human diseases. Since the 2010 landmark reports of small molecules (+)-JQ1 and I-BET762 that target the acetyl-lysine 'reader' Bromodomain and Extra Terminal domain (BET) proteins, there have been relentless efforts to develop epigenetic therapy with small molecules to modulate molecular interactions of epigenome reader domain proteins with PTMs...
July 6, 2017: Current Opinion in Chemical Biology
https://www.readbyqxmd.com/read/28689145/allosteric-regulation-of-epigenetic-modifying-enzymes
#6
REVIEW
Beth E Zucconi, Philip A Cole
Epigenetic enzymes including histone modifying enzymes are key regulators of gene expression in normal and disease processes. Many drug development strategies to target histone modifying enzymes have focused on ligands that bind to enzyme active sites, but allosteric pockets offer potentially attractive opportunities for therapeutic development. Recent biochemical studies have revealed roles for small molecule and peptide ligands binding outside of the active sites in modulating the catalytic activities of histone modifying enzymes...
July 6, 2017: Current Opinion in Chemical Biology
https://www.readbyqxmd.com/read/28662389/recent-progress-in-developing-selective-inhibitors-of-protein-methyltransferases
#7
REVIEW
H Ümit Kaniskan, Jian Jin
Mounting evidence suggests that protein methyltransferases (PMTs), which catalyze methylation of histones as well as non-histone proteins, play a crucial role in diverse biological pathways and human diseases. In particular, PMTs have been recognized as major players in regulating gene expression and chromatin state. There has been an increasingly growing interest in these enzymes as potential therapeutic targets and over the past two years tremendous progress has been made in the discovery of selective, small molecule inhibitors of protein lysine and arginine methyltransferases...
June 26, 2017: Current Opinion in Chemical Biology
https://www.readbyqxmd.com/read/28649012/small-molecule-probes-of-protein-aggregation
#8
REVIEW
Lydia M Young, Alison E Ashcroft, Sheena E Radford
Understanding the mechanisms of amyloid formation and toxicity remain major challenges. Although substantial progress has been made in the development of methods able to identify the species formed during self-assembly and to describe the kinetic mechanisms of aggregation, the structure(s) of non-native species, including potentially toxic oligomers, remain elusive. Moreover, how fibrils contribute to disease remains unclear. Here we review recent advances in the development of small molecules and other reagents that are helping to define the mechanisms of protein aggregation in molecular detail...
June 22, 2017: Current Opinion in Chemical Biology
https://www.readbyqxmd.com/read/28645028/lipid-homeostasis-and-regulated-cell-death
#9
REVIEW
Eran Agmon, Brent R Stockwell
Modern lipidomics analysis paints a dynamic picture of membrane organizations, as changing and adapting lipid assemblies that play an active role in cellular function. This article highlights how the lipid composition of membranes determines specific organelle functions, how homeostatic mechanisms maintain these functions by regulating physical properties of membranes, and how cells disrupt lipid homeostasis to bring about regulated cell death (RCD). These are broad phenomena, and representative examples are reviewed here...
June 20, 2017: Current Opinion in Chemical Biology
https://www.readbyqxmd.com/read/28628894/small-molecule-probes-for-cellular-death-machines
#10
REVIEW
Ying Li, Lihui Qian, Junying Yuan
The past decade has witnessed a significant expansion of our understanding about the regulated cell death mechanisms beyond apoptosis. The application of chemical biological approaches had played a major role in driving these exciting discoveries. The discovery and use of small molecule probes in cell death research has not only revealed significant insights into the regulatory mechanism of cell death but also provided new drug targets and lead drug candidates for developing therapeutics of human diseases with huge unmet need...
June 16, 2017: Current Opinion in Chemical Biology
https://www.readbyqxmd.com/read/28628856/mammalian-synthetic-biology-in-the-age-of-genome-editing-and-personalized-medicine
#11
REVIEW
Patrick Ho, Yvonne Y Chen
The recent expansion of molecular tool kits has propelled synthetic biology toward the design of increasingly sophisticated mammalian systems. Specifically, advances in genome editing, protein engineering, and circuitry design have enabled the programming of cells for diverse applications, including regenerative medicine and cancer immunotherapy. The ease with which molecular and cellular interactions can be harnessed promises to yield novel approaches to elucidate genetic interactions, program cellular functions, and design therapeutic interventions...
June 16, 2017: Current Opinion in Chemical Biology
https://www.readbyqxmd.com/read/28628855/progress-in-programming-spatiotemporal-patterns-and-machine-assembly-in-cell-free-protein-expression-systems
#12
REVIEW
Alexandra M Tayar, Shirley S Daube, Roy H Bar-Ziv
Building biological systems outside the cell is an emerging interdisciplinary research field aimed to study design principles, and to emulate biological functions for technology. Reconstructing programmable cellular functions, from assembly of protein/nucleic-acid machines to spatially distributed systems, requires implementing minimal systems of molecular interactions encoded in genes, source-sink protein expression dynamics, and materials platforms for reaction-diffusion scenarios. Here, we first review how molecular turnover mechanisms, combined with nonlinear interactions and feedback in cell-free gene networks enable programmable dynamic expression patterns in various compartments...
June 16, 2017: Current Opinion in Chemical Biology
https://www.readbyqxmd.com/read/28623730/quantitative-microscopy-based-on-single-molecule-fluorescence
#13
REVIEW
Seung-Ryoung Jung, Bryant S Fujimoto, Daniel T Chiu
Quantitative microscopy is needed to understand reactions or phenomena carried out by biological molecules such as enzymes, receptors, and membrane-localized proteins. Counting the biomolecules of interest in single organelles or cellular compartments is critical in these approaches. In this brief perspective, we focus on the development of quantitative fluorescence microscopies that measure the precise copy numbers of proteins in cellular organelles or purified samples. We introduce recent improvements in quantitative microscopies to overcome undercounting or overcounting errors in certain conditions...
June 14, 2017: Current Opinion in Chemical Biology
https://www.readbyqxmd.com/read/28623722/recent-advances-in-synthetic-biology-for-engineering-isoprenoid-production-in-yeast
#14
REVIEW
Claudia E Vickers, Thomas C Williams, Bingyin Peng, Joel Cherry
Isoprenoids (terpenes/terpenoids) have many useful industrial applications, but are often not produced at industrially viable level in their natural sources. Synthetic biology approaches have been used extensively to reconstruct metabolic pathways in tractable microbial hosts such as yeast and re-engineer pathways and networks to increase yields. Here we review recent advances in this field, focusing on central carbon metabolism engineering to increase precursor supply, re-directing carbon flux for production of C10, C15, or C20 isoprenoids, and chemical decoration of high value diterpenoids (C20)...
June 14, 2017: Current Opinion in Chemical Biology
https://www.readbyqxmd.com/read/28609710/sense-and-sensitivity-in-bioprocessing-detecting-cellular-metabolites-with-biosensors
#15
REVIEW
Linda Dekker, Karen M Polizzi
Biosensors use biological elements to detect or quantify an analyte of interest. In bioprocessing, biosensors are employed to monitor key metabolites. There are two main types: fully biological systems or biological recognition coupled with physical/chemical detection. New developments in chemical biosensors include multiplexed detection using microfluidics. Synthetic biology can be used to engineer new biological biosensors with improved characteristics. Although there have been few biosensors developed for bioprocessing thus far, emerging trends can be applied in the future...
June 10, 2017: Current Opinion in Chemical Biology
https://www.readbyqxmd.com/read/28609675/covalent-inhibitors-an-opportunity-for-rational-target-selectivity
#16
REVIEW
Roman Lagoutte, Remi Patouret, Nicolas Winssinger
There is a resurging interest in compounds that engage their target through covalent interactions. Cysteine's thiol is endowed with enhanced reactivity, making it the nucleophile of choice for covalent engagement with a ligand aligning an electrophilic trap with a cysteine residue in a target of interest. The paucity of cysteine in the proteome coupled to the fact that closely related proteins do not necessarily share a given cysteine residue enable a level of unprecedented rational target selectivity. The recent demonstration that a lysine's amine can also be engaged covalently with a mild electrophile extends the potential of covalent inhibitors...
June 10, 2017: Current Opinion in Chemical Biology
https://www.readbyqxmd.com/read/28605671/targeted-protein-knockdown-using-small-molecule-degraders
#17
REVIEW
Kanak Raina, Craig M Crews
Small molecule probes of biological systems have traditionally been designed to bind to and inhibit the active sites of their protein targets. While this class of pharmacological agents has been broadened by the development of a small number of allosteric and protein-protein interaction (PPI) inhibitors, conventional drug design still excludes 'undruggable' proteins that are neither enzymes nor receptors. Recent years have seen the emergence of new classes of small molecules that can target hitherto undruggable proteins by recruiting the cellular proteostasis machinery to selectively tag them for degradation...
June 9, 2017: Current Opinion in Chemical Biology
https://www.readbyqxmd.com/read/28578260/visualizing-glycans-on-single-cells-and-tissues-visualizing-glycans-on-single-cells-and-tissues
#18
REVIEW
Ben Ovryn, Jie Li, Senlian Hong, Peng Wu
Metabolic oligosaccharide engineering and chemoenzymatic glycan labeling have provided powerful tools to study glycans in living systems and tissue samples. In this review article, we summarize recent advances in this field with a focus on innovative approaches for glycan imaging. The presented applications demonstrate that several of the leading imaging methods, which have revolutionized quantitative cell biology, can be adapted to imaging glycans on single cells and tissues.
June 1, 2017: Current Opinion in Chemical Biology
https://www.readbyqxmd.com/read/28544971/development-of-photostable-fluorophores-for-molecular-imaging
#19
REVIEW
Qinsi Zheng, Luke D Lavis
Advances in fluorescence microscopy promise to unlock details of biological systems with high spatiotemporal precision. These new techniques also place a heavy demand on the 'photon budget'-the number of photons one can extract from a sample. Improving the photostability of small molecule fluorophores using chemistry is a straightforward method for increasing the photon budget. Here, we review the (sometimes sparse) efforts to understand the mechanism of fluorophore photobleaching and recent advances to improve photostability through reducing the propensity for oxidation or through intramolecular triplet-state quenching...
May 22, 2017: Current Opinion in Chemical Biology
https://www.readbyqxmd.com/read/28544970/quantitative-chemical-imaging-with-stimulated-raman-scattering-microscopy
#20
REVIEW
Dan Fu
Chemical imaging plays an increasingly important role in studying heterogeneous biological systems. It combines molecular spectroscopy with high-resolution spatial information to create quantitative images of molecular distributions. Here I summarize recent progress in technical developments and biological applications of a specific chemical imaging technique-stimulated Raman scattering (SRS) microscopy. SRS microscopy allows for a wide range of molecules - both endogenous and exogenous - to be imaged at high spatial and temporal resolution in living cells, tissues, and organisms...
May 22, 2017: Current Opinion in Chemical Biology
journal
journal
33048
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"