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International Journal of Molecular Medicine

Chao Li, Zi Wang, Shuangjiang Chen, Jingyao Zhang, Kai Qu, Chang Liu
MicroRNAs (miRNAs/miRs) are involved in the metastasis of hepatocellular carcinoma (HCC). In the present study, it was demonstrated that miR‑552 was upregulated in HCC tissues. High miR‑552 expression was associated with malignant clinicopathological features and decreased survival rates. The in vitro results indicated that miR‑552 overexpression promoted migration, invasion and epithelial‑mesenchymal transition in Hep3B cells. However, the knockdown of miR‑552 inhibited its oncogenic roles in Huh‑7 cells...
September 17, 2018: International Journal of Molecular Medicine
Yuwei Zhang, Yingquan Luo, Yina Wang, Hong Liu, Yu Yang, Qiong Wang
Intermittent hypoxia/reoxygenation (IHR), characterized by repeated episodes of hypoxia interspersed with periods of reoxygenation, has been found to induce pro‑inflammatory cytokine production and is increasingly recognized as a major pathophysiological factor in various disease processes with distinct cell and molecular responses. The present study is the first, to the best of our knowledge, to investigate the effects of ubiquitin‑specific peptidase 8 (USP8) on IHR‑induced inflammation in renal tubular epithelial cells and examine the underlying mechanism...
September 17, 2018: International Journal of Molecular Medicine
Xiaoqi Zhang, Hao Feng, Juan Du, Jing Sun, Dongfang Li, Tomoka Hasegawa, Norio Amizuka, Minqi Li
Rheumatoid arthritis (RA) is a commonly occurring autoimmune disease. Its defining pathological characteristic is the excessive proliferation of fibroblast‑like synoviocytes (FLS), which is similar to tumor cells and results in a range of clinical problems. As a commonly used antipyretic, analgesic and anti‑inflammatory drug, aspirin is the first‑line treatment for RA. However, its mechanism of action has not been well explained. The goal is to investigate the biological effects of aspirin on primary RA‑FLS and its underlying mechanisms...
September 17, 2018: International Journal of Molecular Medicine
Lin Shen, Li Li, Man Li, Weiling Wang, Wenbin Yin, Wei Liu, Yanyan Hu
The aim of the present study was to investigate the role of the nucleotide‑binding oligomerization domain (NOD) 2 in high glucose (HG)‑induced myocardial apoptosis and fibrosis in mice. Mouse models of diabetes were induced by streptozotocin (STZ). NOD2 expression was knocked down by injection of lentivirus‑mediated short‑hairpin RNA. Alternatively, small interfering RNA‑NOD2 was transfected into cardiomyocytes and cardiac fibroblasts (CFs). A hemodynamic assay was used to assess the cardiac function in the mouse model...
September 14, 2018: International Journal of Molecular Medicine
Joo Wan Kim, You-Suk Lee, Du Jin Seol, Il Je Cho, Sae Kwang Ku, Jae-Suk Choi, Hae-Jeung Lee
Blue honeysuckle (BH, Lonicera caerulea) is used as a traditional medicine in Russia, Japan and China, but is not commonly considered as an edible berry in Europe, USA or Korea. BH has been revealed to decrease serum cholesterol and triacylglycerol (triglyceride or TG) levels through the activation of AMP‑activated protein kinase (AMPK), thus it is expected to be a health functional food and pharmaceutical agent for the prevention of non‑alcoholic liver damage, in addition to effects as a suppressor of hyperlipidemia and as an anti‑obesity agent...
September 14, 2018: International Journal of Molecular Medicine
Qianwen Ma, Yong Tan, Xuan Chen, Shuping Chen, Yuying Sun, Beibei Zhou
The present study aimed to explore the difference in the expression profiles of ovarian microRNA sequences in rats in a light pollution environment and rats in a normal light environment. Rats in the control group were exposed to 12‑h light/dark cycles, while rats in the model group were continuously exposed to 24‑h light. The ovaries were extracted from the two groups of rats, and Illumina HiSeq 2500 high‑throughput sequencing technology was used to detect the differences in microRNA (miRNA) expression among the two groups...
September 13, 2018: International Journal of Molecular Medicine
Miwa Hayashi, Kengo Kuroda, Kohei Ihara, Takahiro Iwaya, Emiko Isogai
Antimicrobial peptides (AMPs) are multifunctional factors with an important role in the innate immune system. Our previous studies revealed that the human cathelicidin LL‑37 and its analog, FF/CAP18, limit the proliferation of colon cancer cell lines. In the present study, the exosomes released by HCT116 cells treated with FF/CAP18 were analyzed. After the treatment, exosomes were isolated from the culture supernatant by ultrafiltration and using the miRCURY™ Exosome Isolation Kit. Membrane vesicles 40‑100‑nm expressing CD63 and CD81 were identified before and after FF/CAP18 treatment...
September 13, 2018: International Journal of Molecular Medicine
Ying Zhou, Ling Li, Jun Qian, Haoyuan Jia, Yubao Cui
Aquaporins (AQPs), or water channel proteins, are highly conserved across species. These transmembrane proteins promote water and solute transport across cell membranes. No AQP‑related proteins have been identified in mites to date. The present study used transcriptomics (RNA‑sequencing) to identify potential AQPs in the mite species Blomia tropicalis. Molecular cloning techniques were then used to obtain the full‑length gene sequences encoding these AQP family members, and bioinformatics analyses were used to categorize them based on similarity to AQPs in other species...
September 13, 2018: International Journal of Molecular Medicine
Kaiwen Bai, Luyi Jiang, Ligen Zhang, Yongwei Zhao, Yi Lu, Jingya Zhu, Jie Cai, Lili Zhang, Tian Wang
The aim of the present study was to evaluate the in vitro free radical scavenging capacity of dimethylglycine sodium (DMG‑Na) and its protective ability against oleic acid hydroperoxide (OAHPx)‑induced oxidative damage in IPEC‑J2 cells. Initially, the free radical scavenging activities of water‑soluble pigments (DMG‑Na, betalain, capsanthin and cyanidin‑3‑rutinoside) were measured and compared with those of Trolox. Subsequently, freshly collected swine blood was mixed with heparin and centrifuged to obtain erythrocytes...
September 13, 2018: International Journal of Molecular Medicine
Wanmin Pei, Yi Zou, Wenting Wang, Lai Wei, Yuan Zhao, Li Li
Recently, α2-adrenoceptors (α2-AR) agonists have been shown to have anti-nociceptive effects and thus may become a promising therapeutic strategy for neuropathic pain. tizanidine is a highly selective α2-AR agonist, but the effect mechanism of tizanidine in neuropathic pain remains largely unknown. The present study investigated whether tizanidine has anti-nociceptive effects in spared nerve injury (SNI) model of neuropathic pain in rats, as well as explored the underlying molecular mechanism. We found that the rats in SNI group showed significantly higher mechanical and thermal hyperalgesia, accompanied with increased production of proinflammatory cytokines including interleukin-1β (IL-1β), IL-6 and tumor necrosis factor-α (TNF-α), as well as the activation of Toll-like receptor 4 (TLR4)/nuclear factor-κB (NF-κB) signaling...
September 13, 2018: International Journal of Molecular Medicine
Hong Zhu, Wei Cao, Peng Zhao, Jieyu Wang, Yuying Qian, Yun Li
Hyperosmotic stress may be initiated during a diverse range pathological circumstances, which in turn results in tissue damage. In this process, the activation of survival signaling, which has the capacity to restore cell homeostasis, determines cell fate. Autophagy is responsible for cell survival and is activated by various pathological stimuli. However, its interplay with hyperosmotic stress and its effect on terminally differentiated cardiac myocytes is unknown. Nuclear factor of activated T‑cells 5 (NFAT5), an osmo‑sensitive transcription factor, mediates the expression of cell survival associated‑genes under hyperosmotic conditions...
September 12, 2018: International Journal of Molecular Medicine
Zuzana Danková, Dušan Braný, Dana Dvorská, Marcela Ňachajová, Roman Fiolka, Marián Grendár, Jozef Hatok, Peter Kubatka, Veronika Holubeková, Erika Halašová, Tibor Bielik, Pavol Žúbor
Endometrial carcinoma is one of the most common tumours in developed countries. In addition to the active role of genetic factors, epigenetic changes also have an important effect. The present study analysed the methylation status of kruppel like factor 4 (KLF4) and heparan sulfate‑glucosamine 3‑sulfotransferase 2 (HS3ST2) genes in three endometrial tissue types for carcinoma prediction. The sample comprised 91 women with histologically‑confirmed endometrial carcinoma (64.16±9.64 years old), 36 women with hyperplasia (53...
September 12, 2018: International Journal of Molecular Medicine
Lili Zhang, Shuguang Zhang, Aihua Li, Anqi Zhang, Shiqian Zhang, Liang Chen
Epithelial ovarian cancer (EOC) is one of the most lethal gynecological malignancies and is known to be associated with the accumulation of various genetic and epigenetic alterations. As a member of the human histone‑lysine N‑methyltransferase SETD1A (SET1)/histone‑lysine N‑methyltransferase 2A (MLL) complexes that are required for full SET1/MLL methyltransferase activity, protein dpy‑30 homolog (DPY30) catalyzes histone H3K4 methylation, and its dysfunction has been associated with the occurrence of cancer...
September 11, 2018: International Journal of Molecular Medicine
Zhu Wang, Jianwen Zhang, Ying Zhang, Qiong Deng, Hui Liang
Breast cancer 1 (BRCA1) and breast cancer 2 (BRCA2) are the most well‑known genes linked to breast cancer and ovarian cancer, which are crucial in DNA repair and transcriptional regulation. The present study aimed to elucidate the expression profiles, mutations and interaction networks of BRCA1 and BRCA2, which may provide insights to reveal the mechanisms of BRCA genes ultimately leading to breast or ovarian tumorigenesis. Bioinformatics analyses were performed in the present study. The mRNA levels of BRCA1 and BRCA2 were evaluated using FIREHOSE analysis, SAGE Genie tools and Oncomine analysis...
September 11, 2018: International Journal of Molecular Medicine
Yuan Yuan, Youwei Zhang, Liang Han, Sanyuan Sun, Yongqian Shu
Ultraviolet radiation resistance‑associated gene (UVRAG) regulates autophagy by promoting the formation and maturation of autophagosomes. The aim of the present study was to investigate the effects of UVRAG and UVRAG‑targeting miRNA on the regulation of autophagy and apoptosis in gastric cancer (GC). TargetScan was used to predict that miR‑183 targets the 3'‑untranslated region (UTR) of UVRAG, while the interaction between miR‑183 and the 3'‑UTR of UVRAG was assessed using a dual luciferase reporter assay...
September 11, 2018: International Journal of Molecular Medicine
Yang Zhou, Tao Hong, Li Tong, Wei Liu, Xueting Yang, Jianghan Luo, Fuling Wang, Jian Li, Lijun Yan
Non‑small cell lung carcinoma (NSCLC) is a life‑threatening malignancy. The level of the cell growth regulator mitogen‑activated protein kinase kinase kinase kinase 3 (MAP4K3) has been shown to be correlated with a high risk of NSCLC recurrence and poor recurrence‑free survival rate. The present study examined the effects of Astragalus polysaccharide (APS) and 10‑hydroxycamptothecin (HCPT), which are associated with marked suppression and dephosphorylation of the MAP4K3/mammalian target of rapamycin (mTOR) signaling pathway, in the H1299 NSCLC cell line...
September 7, 2018: International Journal of Molecular Medicine
Lin Huang, Ping Yuan, Peng Yu, Qiling Kong, Zixuan Xu, Xia Yan, Yang Shen, Juesheng Yang, Rong Wan, Kui Hong, Yanhua Tang, Jinzhu Hu
The O‑linked β‑N‑acetylglucosamine (O‑GlcNAc) modification and autophagy are associated with diabetic myocardial injury, however, the molecular mechanisms between the two processes remain to be fully elucidated. The purpose of the present study was to elucidate the molecular regulation of autophagy by O‑GlcNAc‑modified synaptosomal‑associated protein 29 (SNAP29) in diabetic myocardial injury. A rat model of type I diabetes was established via intraperitoneal injection of streptozotocin (STZ; 55 mg/kg)...
September 7, 2018: International Journal of Molecular Medicine
Kang Xiao, Qi Shi, Wei Zhou, Chen Gao, Cao Chen, Bao-Yun Zhang, Jing Wang, Xiao-Ping Dong
In order to definitively diagnosis sporadic Creutzfeldt‑Jakob disease (sCJD), brain tissue is currently required. Therefore, there is a great need for tests that can detect sCJD in body fluids or other types of tissues. Different variables, including the amount of recombinant celluar prion protein (rPrPC), salt, cleaning surfactants and thioflavin T (ThT), in human cerebrospinal fluid (CSF) were evaluated. The reagent concentrations of 1X PBS, 170 mM NaCl, 1 mM EDTA, 0.01 mM ThT and 0.001% SDS, and the amounts of 10 µg rPrPC and 10 µl CSF were considered to be optimal for the real‑time quaking‑induced conversion (RT‑QuIC) assay...
September 7, 2018: International Journal of Molecular Medicine
Guangdi Li, Rudan Zhou, Xueling Zhao, Riguang Liu, Chuan Ye
In the above paper, the authors realize that an error inadvertently appeared in the funding section on p. 895. Where "This study was supported by the National Natural Science Foundation of China Science Fund Project (grant no. 30960389 and 8176010097)" was written, the application number 8176010097 was featured incorrectly: This should have been written as grant no. 81760030. Therefore, this sentence in the funding section should have read as follows: "This study was supported by the National Natural Science Foundation of China Science Fund Project (grant nos...
August 27, 2018: International Journal of Molecular Medicine
Peipei Chen, Ruiyu Zhang, Lijun Mou, Xuewang Li, Yan Qin, Xuemei Li
Hyperlipidemia is a key clinical feature in patients with nephrotic syndrome (NS) that is associated with the incidence of cardiovascular events. Recent studies have suggested that the disorders of triglycerides, gluconeogenesis and liver glucose metabolism are associated with the abnormal transcription of clock genes. However, changes to the circadian rhythm of blood lipids in NS require further exploration, and the effects of NS on the hepatic clock system remain to be elucidated. In the present study, the impaired diurnal rhythm of the hepatic core clock genes (BMAL1, CLOCK, CRY1, CRY2, PER1 and PER2) significantly induced circadian rhythm abnormalities in liver‑specific clock‑controlled genes (LXR, CYP7A1, SREBP‑1, ABCA1, DEC1 and DEC2; all P<0...
August 22, 2018: International Journal of Molecular Medicine
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