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Journal of Oral Science

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https://www.readbyqxmd.com/read/29743385/formation-of-chemical-bonds-on-zirconia-surfaces-with-acidic-functional-monomers
#1
Saiji Shimoe, Isao Hirata, Mai Otaku, Hideo Matsumura, Koichi Kato, Takahiro Satoda
We investigated the chemical interaction between zirconia surfaces and functional monomers using X-ray photoelectron spectroscopy (XPS). Two types of zirconia disks cleaned with piranha solution were treated with one of two phosphate primers (Alloy Primer, Clearfil Ceramic Primer) or a carboxylic primer (Super-Bond C&B Monomer), and rinsed 3 times with acetone. XPS analysis revealed that phosphorus was incorporated into zirconia when the surface was treated with a primer containing phosphate monomer (10-methacryloyloxydecyl dihydrogen phosphate; MDP)...
May 10, 2018: Journal of Oral Science
https://www.readbyqxmd.com/read/29743384/temporal-change-in-the-occlusal-vertical-dimension-and-its-involvement-in-modulation-of-jaw-movement-in-bite-reduced-animals
#2
Hiroshi Matoba, Hayato Kanayama, Takafumi Kato, Mohammad Z Hossain, Junichi Kitagawa, Yoshie Takehana, Kazuhiro Yamada, Yuji Masuda
The occlusal vertical dimension (OVD) in guinea pigs is maintained by tooth eruption and grinding. It has been reported that the experimentally raised OVD recovers to the innate OVD within a few days in guinea pigs. However, the mechanisms underlying OVD adjustment are not entirely understood. This study thus aimed to clarify whether the experimentally reduced OVD would recover. Bite-reduced guinea pigs were created by applying bilateral intermaxillary elastics for 10 days. Guinea pigs without elastics were used as a control...
May 10, 2018: Journal of Oral Science
https://www.readbyqxmd.com/read/29743383/five-year-outcome-of-a-retrospective-cohort-study-comparing-smokers-vs-nonsmokers-with-full-arch-mandibular-implant-supported-rehabilitation-using-the-all-on-4-concept
#3
Paulo S Maló, Miguel A de Araújo Nobre, Ana S Ferro, Gonçalo G Parreira
The aim of this study was to compare the 5-year outcome of full-arch mandibular fixed prosthetic rehabilitation using the All-on-4 concept in smoking and nonsmoking patients. This retrospective cohort study included 200 patients (n = 100 smokers, n = 100 nonsmokers), 119 women and 81 men, with an average age of 53.7 years, rehabilitated in immediate function with 800 implants. Implant cumulative survival rate estimation (Kaplan-Meier with log-rank test) and marginal bone resorption (MBR) at 5 years (Mann-Whitney test) were compared between both groups...
May 10, 2018: Journal of Oral Science
https://www.readbyqxmd.com/read/29695671/marginal-sealing-of-relocated-cervical-margins-of-mesio-occluso-distal-overlays
#4
Serhat Köken, Jelena Juloski, Roberto Sorrentino, Simone Grandini, Marco Ferrari
We investigated the effect of cervical marginal relocation (CMR) on marginal sealing with two different viscosity resin composites, before adhesive cementation of composite computer-aided design/computer-assisted manufacture mesio-occluso-distal (MOD) overlays. Standardized MOD cavities prepared in 39 human molars were randomly assigned to three groups. The proximal margins on the mesial side were located 1 mm below the cementoenamel junction. On the distal side of the tooth, the margins were located 1 mm above the cementoenamel junction...
April 25, 2018: Journal of Oral Science
https://www.readbyqxmd.com/read/29695670/peripheral-clear-cell-variant-of-calcifying-epithelial-odontogenic-tumor-devoid-of-calcification
#5
Jun Kurihara, Shiro Mori, Mariko Oikawa, Hiroyuki Kumamoto, Tetsu Takahashi
A clear cell variant of calcifying epithelial odontogenic tumor (CCEOT) affecting an extraosseous site is described. A 60-year-old male patient presented with gingival swelling on the lingual side of the anterior mandible. The results of biopsy suggested clear cell odontogenic carcinoma, and marginal resection of the mandible was performed. The resected specimen was composed of eosinophilic and clear cells with deposits of amyloid-like material. The clear cells exhibited granules that were positive for PAS...
April 25, 2018: Journal of Oral Science
https://www.readbyqxmd.com/read/29657251/laminin-1-acts-as-an-adhesive-for-odontoblast-like-cells-and-promotes-their-differentiation-toward-a-hard-tissue-forming-phenotype
#6
Jia Tang, Takashi Saito
The present study was designed to investigate the effect of laminin-1 (LN-1 or LN-111) on an odontoblast-like cell line, MDPC-23. Wells of non-treated polystyrene plates were coated with various concentrations of LN-1 (0.1, 1, 10, and 100 µg/mL) and left to dry for 2 days. Water-coated surfaces were used as controls. MDPC-23 cell proliferation, differentiation and mineralization were evaluated in terms of the CCK-8 assay, ALP activity, real-time RT-PCR and Alizarin red staining. The data indicated that LN-1 promoted the proliferation of MDPC-23 cells in a concentration-dependent manner...
April 16, 2018: Journal of Oral Science
https://www.readbyqxmd.com/read/29657250/tgf%C3%AE-1-induced-amelotin-gene-expression-is-downregulated-by-bax-expression-in-mouse-gingival-epithelial-cells
#7
Yohei Nakayama, Sari Matsui, Keisuke Noda, Mizuho Yamazaki, Yasunobu Iwai, Bernhard Ganss, Yorimasa Ogata
Amelotin (AMTN) is induced upon initiation of apoptosis by transforming growth factor beta1 (TGFβ1) and is mediated by Smad3 in gingival epithelial cells (GE1 cells). This upregulation of AMTN gene expression is temporary, and the mechanism responsible is still unclear. The present study investigated the transcriptional downregulation of TGFβ1-induced AMTN gene expression in GE1 cells during the progression of apoptosis. To examine time-dependent changes in the levels of AMTN, Smad3 and Bax mRNA induced by TGFβ1, real-time PCR analyses were performed...
April 16, 2018: Journal of Oral Science
https://www.readbyqxmd.com/read/29657249/microcomputed-tomographic-evaluation-of-techniques-for-warm-gutta-percha-obturation
#8
Hisashi Suguro, Osamu Takeichi, Makoto Hayashi, Teinosuke Okamura, Akiko Hira, Yoriyuki Hirano, Bunnai Ogiso
Transparent epoxy resin root canal models were used to evaluate vertical condensation techniques for obturating lateral canals. The root canal model was configured with a straight main root canal and four right-angled lateral canals at 1.0 and 3.0 mm from the apex. Root canal obturation was performed with Thermafil, Obtura II, or NT condenser. Obturation volume in lateral canals was measured by three-dimensional microcomputed tomography, and one-way analysis of variance was used to analyze differences between groups...
April 16, 2018: Journal of Oral Science
https://www.readbyqxmd.com/read/29503395/inhibition-of-2-arachydonoylgycerol-degradation-attenuates-orofacial-neuropathic-pain-in-trigeminal-nerve-injured-mice
#9
Rantaro Kamimura, Mohammad Z Hossain, Shumpei Unno, Hiroshi Ando, Yuji Masuda, Kojiro Takahashi, Masanori Otake, Isao Saito, Junichi Kitagawa
Current therapeutics are not effective for orofacial neuropathic pain, and better options are needed. The present study used inferior orbital nerve (ION)-injured mice to investigate the effect of inhibiting monoacylglycerol lipase (MAGL), an enzyme that degrades the major endocannabinoid 2-arachydonoylgycerol (2-AG) in orofacial neuropathic pain. The head-withdrawal threshold to mechanical stimulation of the whisker pad was reduced on days 3, 5, and 7 after ION injury. Injection of JZL184, a selective inhibitor of MAGL, on day 7 after ION injury attenuated the reduction in head-withdrawal threshold at 2 h after administration...
March 24, 2018: Journal of Oral Science
https://www.readbyqxmd.com/read/29479030/involvement-of-transient-receptor-potential-vanilloid-1-channel-expression-in-orofacial-cutaneous-hypersensitivity-following-tooth-pulp-inflammation
#10
Tetsuro Watase, Kohei Shimizu, Hiroki Komiya, Kinuyo Ohara, Koichi Iwata, Bunnai Ogiso
A study was conducted to evaluate the mechanisms underlying ectopic orofacial pain associated with tooth pulp inflammation in rats. We observed a significant decrease in the head withdrawal threshold (HWT) response to mechanical and heat stimuli applied to the ipsilateral facial skin upon application of complete Freund's adjuvant (CFA) to the upper first molar (M1TP) in comparison to application of vehicle. A large number of trigeminal ganglion (TG) neurons showed transient receptor potential vanilloid 1 (TRPV1) immunoreactivity (IR), and some of them were retrogradely labeled with fluorogold injected into the facial skin...
March 24, 2018: Journal of Oral Science
https://www.readbyqxmd.com/read/29479029/cytogenetic-genomic-and-epigenetic-characterization-of-the-hsc-3-tongue-cell-line-with-lymph-node-metastasis
#11
Ilda P Ribeiro, Joana M Rodrigues, Alexandra Mascarenhas, Nadezda Kosyakova, Francisco Caramelo, Thomas Liehr, Joana B Melo, Isabel M Carreira
Oral carcinoma develops from squamous epithelial cells by the acquisition of multiple (epi) genetic alterations that target different genes and molecular pathways. Herein, we performed a comprehensive genomic and epigenetic characterization of the HSC-3 cell line through karyotyping, multicolor fluorescence in situ hybridization, array comparative genomic hybridization, and methylation-specific multiplex ligation-dependent probe amplification. HSC-3 turned out to be a near-triploid cell line with a modal number of 61 chromosomes...
March 24, 2018: Journal of Oral Science
https://www.readbyqxmd.com/read/29479028/effect-of-collagenase-concentration-on-the-isolation-of-small-adipocytes-from-human-buccal-fat-pad
#12
Niina Tsurumachi, Daisuke Akita, Koichiro Kano, Taro Matsumoto, Taku Toriumi, Tomohiko Kazama, Yoshinao Oki, Yoko Saito-Tamura, Morio Tonogi, Noriyoshi Shimizu, Masaki Honda
Dedifferentiated fat (DFAT) cells were isolated from mature adipocytes using the ceiling culture method. Recently, we successfully isolated DFAT cells from adipocytes with a relatively small size (<40 μm). DFAT cells have a higher osteogenic potential than that of medium adipocytes. Therefore, the objective of this study was to determine the optimal concentration of collagenase solution for isolating small adipocytes from human buccal fat pads (BFPs). Four concentrations of collagenase solution (0.01%, 0...
March 24, 2018: Journal of Oral Science
https://www.readbyqxmd.com/read/29479027/a-micro-ct-study-of-the-greater-palatine-foramen-in-human-skulls
#13
Mia-Michaela Beetge, Vladimir S Todorovic, Anna Oettlé, Jakobus Hoffman, Andre W van Zyl
The greater palatine foramen (GPF) is an important anatomical landmark and has substantial clinical relevance in dental surgery. Knowledge of its precise location and dimensions is required for proper planning of surgical procedures involving the posterior maxilla. We used microfocus computed tomography to determine the location and dimensions of the GPF, and any sex and race variations in those measurements, in 77 human skulls scanned at the South African Nuclear Energy Corporation. Specialized software was used for three-dimensional rendering, segmentation, and visualization of the reconstructed volume data...
March 24, 2018: Journal of Oral Science
https://www.readbyqxmd.com/read/29479026/histologic-comparison-of-direct-pulp-capping-of-rat-molars-with-mta-and-different-concentrations-of-simvastatin-gel
#14
Omid Dianat, Fatemeh Mashhadiabbas, Zohreh Ahangari, Sara Saedi, Saeed Reza Motamedian
Previous in vitro studies have suggested that simvastatin can be used as a direct pulp capping material due to its ability to induce odontoblastic differentiation and angiogenesis. The aim of this animal study was to evaluate the pulpal response to mineral trioxide aggregate (MTA) and four concentrations of simvastatin/MTA in combination. The study was conducted in two stages using four different simvastatin concentrations and MTA as a capping material for rat maxillary molars. The grades of inflammation and continuity of dentin formation were evaluated in hematoxylin and eosin (HE)-stained samples...
March 24, 2018: Journal of Oral Science
https://www.readbyqxmd.com/read/29375100/biological-evaluation-of-subgingivally-placed-direct-resin-composite-materials
#15
Cornelia Frese, Diana Wolff, Tanja Krüger, Hans J Staehle, Christopher J Lux, Ralf Erber
Placement of composite resin restorations in deep subgingival cavities can damage surrounding soft tissues. In addition, commonly used resin-based composites (RBCs) might interfere with wound healing and periodontal health. To clarify cellular interactions with RBCs, we used an MTT assay to investigate adhesion of primary human gingival fibroblasts and human osteoblasts (hFOB 1.19) on five RBC materials with and without surface modifications (alumina blasting with 50- or 110-μm Al2 O3 ). In addition, high-performance liquid chromatography (HPLC) was used to determine release of resin monomers from RBCs after 1 h, 1 day, and 7 days...
March 24, 2018: Journal of Oral Science
https://www.readbyqxmd.com/read/29375099/simulated-localized-wear-of-resin-luting-cements-for-universal-adhesive-systems-with-different-curing-mode
#16
Akimasa Tsujimoto, Wayne W Barkmeier, Toshiki Takamizawa, Hidehiko Watanabe, William W Johnson, Mark A Latta, Masashi Miyazaki
This study evaluated the simulated localized wear of resin luting cements for universal adhesive systems using different curing modes. Five resin luting cements for universal adhesive systems were evaluated and subsequently subjected to wear challenge in a Leinfelder-Suzuki wear simulation device. Overall, 20 specimens from each resin luting cement were photo-cured for 40 s (dual-cure group), and 20 specimens of each material were not photo-cured (chemical-cure group). Simulated localized wear was generated using a stainless steel ball-bearing antagonist in water slurry of polymethylmethacrylate beads...
March 24, 2018: Journal of Oral Science
https://www.readbyqxmd.com/read/29375098/evaluation-of-an-innovative-oral-brush-for-potential-applications-using-liquid-based-cytology
#17
Omar Kujan, Michael N Pemberton, Martin Schwarz, Philip Sloan
The present study was conducted to assess the applicability of liquid-based cytology (LBC) using an innovative oral brush, Orcellex. Fifty healthy volunteers were recruited. From each subject, four samples were collected using "Orcellex" from apparently normal oral mucosal sites. A plastic spatula was also used to obtain an additional sample. Data on the tolerability and acceptability of the Orcellex were collected from the subjects, together with assessments of the adequacy of LBC slide preparations for cellularity, preparation quality, and the types of cells observed...
March 24, 2018: Journal of Oral Science
https://www.readbyqxmd.com/read/29311501/retrospective-clinical-evaluation-of-posterior-monolithic-zirconia-restorations-after-1-to-3-5-years-of-clinical-service
#18
Hiroto Gunge, Yoichiro Ogino, Masafumi Kihara, Yoshihiro Tsukiyama, Kiyoshi Koyano
This retrospective study analyzed clinical outcomes of monolithic zirconia restorations (MZRs) and factors related to restoration success. Patient records were searched to identify those provided MZRs (Cercon ht) for premolars or molars between April 2012 and March 2016. All MZRs were placed according to a standardized protocol. Kaplan-Meier analysis was used to assess MZR performance and failure after recall appointments at 1 year or later. In total, 101 patients received 148 MZRs. Mean duration of follow-up was 25...
March 24, 2018: Journal of Oral Science
https://www.readbyqxmd.com/read/29576581/aggressive-periodontitis-presents-a-higher-degree-of-bilateral-symmetry-in-comparison-with-chronic-periodontitis
#19
Liana F Brito, Zuila A Taboza, Virginia R Silveira, Flavia A Furlaneto, Cassiano K Rosing, Rodrigo O Rego
The purpose of this cross-sectional study was to evaluate the occurrence of bilateral symmetry in the distribution of clinical parameters in subjects with generalized aggressive periodontitis (GAP) and severe chronic periodontitis (SCP). The sample comprised 53 subjects with GAP and 33 with SCP. Probing depth (PD) and clinical attachment loss (CAL) were recorded from both buccal and lingual interproximal sites of incisors and molars. The symmetry of periodontal destruction was analyzed in terms of intraclass coefficient correlations (ICC) for pairs of contralateral sites at which PD and/or CAL was ≥5 mm at one of the sites of the subjects in each group...
2018: Journal of Oral Science
https://www.readbyqxmd.com/read/29576580/in-vitro-cytotoxic-evaluation-of-novel-fast-setting-calcium-silicate-cement-compositions-and-dental-materials-using-colorimetric-methyl-thiazolyl-tetrazolium-assay
#20
Bahram Ranjkesh, Flemming Isidor, David Christian Evar Kraft, Henrik Løvschall
A novel fast-setting calcium silicate cement with fluoride (CSC) has been developed for potential application in tooth crowns. This study compared the cytotoxicity of CSC compositions and a variety of dental materials. We tested CSC compositions (Protooth), MTA, Biodentine, Ketac Molar, Fuji II LC, Vitrebond, DeTrey Zinc, Dycal, and IRM, DMEM (negative control) and 1% NaOCl (positive control). After setting of cements for 24 h, specimens were immersed in DMEM for 24 h to obtain material elutes. The elutes were serially diluted in serum-free DMEM to obtain three dilutions...
2018: Journal of Oral Science
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