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Journal of Oral Science

Satoshi Shimizu, Yohei Sato, Mai Shirai, Toshimitsu Matsumoto, Minoru Abe, Chikahiro Ohkubo
To evaluate the occlusion accuracy of a novel impression technique, excessively high occlusion and the occlusal contact area during lateral movements were compared between metal restorations (restorations) and removable partial dentures (RPDs) fabricated using conventional and novel techniques. Both restorations and RPDs were fabricated on the simulation model with the precise displacement of the remaining teeth and soft tissue. For the novel technique, functionally generated path (FGP) recording and impression under occlusal force were simultaneously performed using a custom tray with an FGP table...
August 30, 2018: Journal of Oral Science
Keisuke Noda, Mizuho Yamazaki, Yasunobu Iwai, Sari Matsui, Ayako Kato, Hideki Takai, Yohei Nakayama, Yorimasa Ogata
Amelotin (AMTN) is an enamel protein expressed in maturation-stage ameloblasts and junctional epithelium. To clarify the transcriptional regulation of the AMTN gene by interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α), we conducted real-time PCR, Western blotting, transient transfection analyses with luciferase constructs including various lengths of the mouse AMTN gene promoter, and gel shift and chromatin immunoprecipitation assays using mouse gingival epithelial GE1 cells. The levels of AMTN mRNA and protein in GE1 cells were increased after 6 h of stimulation with IL-1β (1 ng/mL) and TNF-α (10 ng/mL)...
August 30, 2018: Journal of Oral Science
Hirofumi Mizuno, Shinsuke Mizutani, Daisuke Ekuni, Ayano Tabata-Taniguchi, Takayuki Maruyama, Aya Yokoi, Chie Omori, Kazuyoshi Shimizu, Hiroshi Morimatsu, Yasuhiro Shirakawa, Manabu Morita
Using a controlled pre/post study design, we investigated the effects of professional mechanical cleaning of the oral cavity with benzethonium chloride, interdental brushes, and hydrogen peroxide on the number of oral bacteria and postoperative complications among esophageal cancer patients in an intensive care unit. Before surgery, 44 patients with esophageal cancer were recruited at Okayama Hospital from January through August 2015. The control group (n = 23) received routine oral hygiene care in the intensive care unit...
August 30, 2018: Journal of Oral Science
Katsumitsu Shimada, Takanaga Ochiai, Fa-Chih Shen, Hiromasa Hasegawa
In oral lichen planus, extracellular matrix and basal cells are damaged by T-lymphocytes. As a consequence, changes in expression of collagen fibers within the connective tissue and cytoskeletons of the epithelial tissue can be observed. With the goal of examining the characteristic changes undergone by basal cells as a consequence of T-lymphocytes damage in oral lichen planus, we investigated protein expression in the epithelial-connective junction. We selected 20 cases of oral lichen planus and 5 control samples of buccal mucosa...
August 25, 2018: Journal of Oral Science
Serina Taguchi, Futoshi Komine, Kei Kubochi, Ryosuke Fushiki, Fumiaki Kimura, Hideo Matsumura
This study examined the effect of silane and phosphate functional monomer on bond strengths between a resin-based luting agent joined to a lithium disilicate ceramic (IPS e.max) and silica (quartz) materials. The e.max and quartz specimens were assigned to 6 groups with different priming/bonding agents, namely, Clearfil Porcelain Bond Activator, Clearfil Photo Bond, Clearfil Photo Bond Universal with Clearfil Porcelain Bond Activator, Clearfil Photo Bond Catalyst with Clearfil Porcelain Bond Activator, Clearfil Photo Bond with Clearfil Porcelain Bond Activator, and unprimed...
August 25, 2018: Journal of Oral Science
Miho Obayashi-Ishii, Satsuki Saito, Daisuke Omagari, Masatake Asano, Tomohiko Ishigami
Magnetic attachments are commonly used for overdentures. The deleterious effects of exposure to magnetic flux on human health have not been substantiated so far; nevertheless, there is a need to understand the extent of magnetic field exposure in the oral area resulting from the use of magnetic attachments. The purpose of this study was to investigate the influence of a magnetic field on oral squamous cell carcinoma. Tumor cells cultured on a magnetic plate were compared with those not cultured on a magnetic plate (controls)...
August 25, 2018: Journal of Oral Science
Asako Suzuki, Masayasu Ito, Yasuhiko Kawai
Clear magnetic resonance imaging (MRI) is required to diagnose tongue cancer. However, the absence of occlusal support may cause tongue movements which are known to introduce artifacts on the MR image. This pilot study compared the manifest of artifacts from the tongue at rest and during motion using luminance standard deviation (LSD) to quantify the artifacts, in dentulous subjects. Participants were ten dentulous participants (5 males, 5 females; age 31.50 ± 8.38 years) with occlusal support. MRI was conducted with the tongue at rest and during lateral movement...
August 25, 2018: Journal of Oral Science
Erhan Dursun, Güliz N Güncü, Ceyda K Dursun, Arlin Kiremitçi, Erdem Karabulut, Ferda A Akalın
The aim of this study was to evaluate the clinical root coverage results of subepithelial connective tissue grafts (SCTG) performed on teeth with gingival recessions and non-carious cervical lesions (NCCLs) that were restored with cervical fillings and compare the results of two different filling materials: resin-modified glass ionomer (RMGIC) and nano-ionomer cements (NIC). A total of 54 teeth with Miller Class I gingival recessions with or without NCCLs in 36 patients (28 females, 8 males) were treated with SCTGs...
August 25, 2018: Journal of Oral Science
Shigenori Hirai, Yusuke Hayashi, Motohiro Ito, Toshihiko Amemiya, Ko Dezawa, Yoshinori Arai, Ken-Ichiro Ejima, Shigeki Shimba, Kazuya Honda
Brain and muscle Arnt-like protein-1 (BMAL1) knockout mice exhibit accelerated aging, abnormal glucose metabolism, and impaired adipocyte differentiation, among other phenotypes, which are effects associated with the BMAL1 gene. No study has investigated temporal changes in the deformation of the mandibular condyle and the presence of calcification in areas surrounding the mandibular condyle. In a study of 12 C57/BL strain mice under inhalation anesthesia, we collected images of the mandibular condyle at 6 weeks after birth and then every 5 weeks from 10 to 25 weeks after birth...
August 25, 2018: Journal of Oral Science
Hiroshi Kudo, Osamu Takeichi, Kosuke Makino, Keisuke Hatori, Bunnai Ogiso
Silent information regulator 2 homolog 1 (SIRT1) inhibits oxidative injury and has anti-inflammatory effects. SIRT1 may be involved in healing of periapical periodontitis; however, SIRT1 expression in periapical periodontitis lesions has not been investigated. This study evaluated SIRT1 expression and a marker of oxidative stress-8-hydroxy-2'-deoxyguanosine (8-OHdG)-in periapical granulomas. First, we used real-time polymerase chain reaction to determine whether U-937 monocytes express SIRT1. U-937 cells treated with the SIRT1 activator resveratrol exhibited the highest SIRT1 mRNA level after 6-h incubation...
August 12, 2018: Journal of Oral Science
Takanobu Yamamoto, Akira Hasuike, Ryosuke Koshi, Yasumasa Ozawa, Manami Ozaki, Tatsuya Kubota, Shuichi Sato
We used radiological and histological analyses to evaluate the effects of mechanical barrier permeability in a rat model of calvarial guided bone augmentation (GBA). The calvaria of 20 rats were exposed, and one of four types of plastic caps (an occlusive cylindrical plastic cap; a plastic cap with no top; a plastic cap with three holes; and a plastic cap with four holes) was randomly placed on both sides. Newly generated bone in the plastic caps was evaluated with micro-computed tomography (micro-CT) and histological analysis...
August 12, 2018: Journal of Oral Science
Kallapat Tansriratanawong, Pawinee Wongwan, Hiroshi Ishikawa, Taka Nakahara, Kanet Wongravee
Calcium hydrogen phosphate with a hydroxyapatite-like surface (CHP-HA) is a novel synthesized compound designed to overcome the limitations of bioactive ceramics. It was originally applied as nano-sized HA strips covering core plates to enhance the degree of interfacial attachment. The objective of the present study was to examine the cellular attachment, proliferation, and osteogenic differentiation of periodontal ligament stem cells (PDLSCs) on a CHP-HA substrate in comparison with conventional nanohydroxyapatite (NanoHA)...
August 12, 2018: Journal of Oral Science
Takahiro Mitsui, Masahiko Saito, Ryô Harasawa
Dietary nitrate has several beneficial effects, including blood pressure reduction and improved oxygen consumption efficiency, but in order to do so it must first be reduced to nitrite by oral bacteria. Veillonella spp., a strictly anaerobic group, are the most prevalent nitrate-reducing bacteria in the oral cavity. In response to some early studies that have hinted at inter- and intra-individual variation in salivary nitrate-nitrite conversion capacity, the purpose of the present study was to investigate the incidence of and variation in the Veillonella species V...
August 12, 2018: Journal of Oral Science
Indre Graunaite, Greta Lodiene, Odeta Arandarcikaite, Audrius Pukalskas, Vita Machiulskiene
This in vitro study aimed to detect leaching components from an epoxy resin- and a methacrylate-based endodontic sealer and correlate them to cytotoxicity induced by material extracts for up to 36 weeks. We qualitatively determined the substances released by aged AH Plus and RealSeal SE specimens at seven intervals between 0 and 36 weeks. Quantification was performed by ultra-performance liquid chromatography/mass spectrometry (UPLC/MS). We determined the viability of murine macrophage J774 cells after 24 h exposure to material extracts, at each interval, using a fluorescence staining/microscopy method...
August 12, 2018: Journal of Oral Science
Stefano Mummolo, Alessandro Nota, Maria Elena De Felice, Domenico Marcattili, Simona Tecco, Giuseppe Marzo
This study investigated differences in periodontal health variables between buccally impacted maxillary canines (BIMC) and palatally impacted maxillary canines (PIMC) after surgical-orthodontic treatment with open technique. Nineteen patients were enrolled: 10 with unilateral BIMC (5 men, 5 women; mean age 18.50 ± 1.96 years) and 9 with unilateral PIMC (4 men, 5 women; mean age 19.44 ± 2.40 years). Probing depth and keratinized tissue were recorded 12 months after surgical-orthodontic treatment, and the differences between the 2 sides were analyzed as primary outcomes...
July 9, 2018: Journal of Oral Science
Hironori Mori, Kazunori Hamamura, Shoyoku Yo, Kosuke Hamajima, Kenji Ootani, Masaki Honda, Kyoko Ishizuka, Hisataka Kondo, Kenjiro Tanaka, Daisuke Kodama, Takao Hirai, Ken Miyazawa, Shigemi Goto, Akifumi Togari
Dental pulp is known to play crucial roles in homeostasis of teeth and periodontal tissue. Although resorption of bone around the roots of nonvital teeth is occasionally observed in clinical practice, little is known about the role of dental pulp in osteoclastogenesis. Here we evaluated the effects of conditioned medium (CM) from rat dental pulp on osteoclastogenesis. It was found that the CM reduced the number of tartrate-resistant acid phosphatase (TRAP)-positive multinucleated osteoclasts, but did not alter the mRNA levels of nuclear factor of activated T-cells, cytoplasmic 1 and TRAP...
July 9, 2018: Journal of Oral Science
Ryosuke Murayama, Yuko Nagura, Kabun Yamauchi, Nobuyuki Moritake, Masayoshi Iino, Ryo Ishii, Hiroyasu Kurokawa, Masashi Miyazaki, Yumiko Hosoya
We used optical coherence tomography to examine the effect of a coating material containing surface reaction-type pre-reacted glass-ionomer (S-PRG) filler on primary enamel demineralization in 18 extracted human primary teeth. The pulp was removed, and each tooth was ultrasonically cleaned with distilled water. Six teeth were treated with 0.1-M lactic acid buffer solution (De group). In the second group (n = 6), a thin film of coating material was applied before demineralization (PRG group). A third group (Control group; n = 6) was maintained in artificial saliva...
July 9, 2018: Journal of Oral Science
Saiji Shimoe, Isao Hirata, Mai Otaku, Hideo Matsumura, Koichi Kato, Takahiro Satoda
We investigated the chemical interaction between zirconia surfaces and functional monomers using X-ray photoelectron spectroscopy (XPS). Two types of zirconia disks cleaned with piranha solution were treated with one of two phosphate primers (Alloy Primer, Clearfil Ceramic Primer) or a carboxylic primer (Super-Bond C&B Monomer), and rinsed 3 times with acetone. XPS analysis revealed that phosphorus was incorporated into zirconia when the surface was treated with a primer containing phosphate monomer (10-methacryloyloxydecyl dihydrogen phosphate; MDP)...
June 20, 2018: Journal of Oral Science
Hiroshi Matoba, Hayato Kanayama, Takafumi Kato, Mohammad Z Hossain, Junichi Kitagawa, Yoshie Takehana, Kazuhiro Yamada, Yuji Masuda
The occlusal vertical dimension (OVD) in guinea pigs is maintained by tooth eruption and grinding. It has been reported that the experimentally raised OVD recovers to the innate OVD within a few days in guinea pigs. However, the mechanisms underlying OVD adjustment are not entirely understood. This study thus aimed to clarify whether the experimentally reduced OVD would recover. Bite-reduced guinea pigs were created by applying bilateral intermaxillary elastics for 10 days. Guinea pigs without elastics were used as a control...
June 20, 2018: Journal of Oral Science
Paulo S Maló, Miguel A de Araújo Nobre, Ana S Ferro, Gonçalo G Parreira
The aim of this study was to compare the 5-year outcome of full-arch mandibular fixed prosthetic rehabilitation using the All-on-4 concept in smoking and nonsmoking patients. This retrospective cohort study included 200 patients (n = 100 smokers, n = 100 nonsmokers), 119 women and 81 men, with an average age of 53.7 years, rehabilitated in immediate function with 800 implants. Implant cumulative survival rate estimation (Kaplan-Meier with log-rank test) and marginal bone resorption (MBR) at 5 years (Mann-Whitney test) were compared between both groups...
June 20, 2018: Journal of Oral Science
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