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Protoplasma

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https://www.readbyqxmd.com/read/28808798/genetic-engineering-approach-using-early-vinca-alkaloid-biosynthesis-genes-led-to-increased-tryptamine-and-terpenoid-indole-alkaloids-biosynthesis-in-differentiating-cultures-of-catharanthus-roseus
#1
Abhishek Sharma, Priyanka Verma, Archana Mathur, Ajay Kumar Mathur
Catharanthus roseus today occupies the central position in ongoing metabolic engineering efforts in medicinal plants. The entire multi-step biogenetic pathway of its very expensive anticancerous alkaloids vinblastine and vincristine is fairly very well dissected at biochemical and gene levels except the pathway steps leading to biosynthesis of monomeric alkaloid catharanthine and tabersonine. In order to enhance the plant-based productivity of these pharma molecules for the drug industry, cell and tissue cultures of C...
August 14, 2017: Protoplasma
https://www.readbyqxmd.com/read/28803402/actin-filaments-regulate-the-adhesion-between-the-plasma-membrane-and-the-cell-wall-of-tobacco-guard-cells
#2
Qin Yu, Jing-Jing Ren, Lan-Jing Kong, Xiu-Ling Wang
During the opening and closing of stomata, guard cells undergo rapid and reversible changes in their volume and shape, which affects the adhesion of the plasma membrane (PM) to the cell wall (CW). The dynamics of actin filaments in guard cells are involved in stomatal movement by regulating structural changes and intracellular signaling. However, it is unclear whether actin dynamics regulate the adhesion of the PM to the CW. In this study, we investigated the relationship between actin dynamics and PM-CW adhesion by the hyperosmotic-induced plasmolysis of tobacco guard cells...
August 13, 2017: Protoplasma
https://www.readbyqxmd.com/read/28779305/candidates-of-trichocyst-matrix-proteins-of-the-dinoflagellate-oxyrrhis-marina
#3
Erhard Rhiel, Lars Wöhlbrand, Ralf Rabus, Sonja Voget
Trichocysts are a common cell organelle of ciliates and dinoflagellates. They are composed of trichocyst matrix proteins and have been intensely investigated and characterized in ciliates. Here, for the first time, data have been obtained for trichocyst matrix proteins of a dinoflagellate. A DELTA-BLAST search using 14 available and complete amino acid sequences of mature trichocyst matrix proteins of the ciliate Paramecium tetraurelia resulted in 16 hits for the dinoflagellate Oxyrrhis marina when the E values and bit values to be scored were <10(-4) and >40...
August 4, 2017: Protoplasma
https://www.readbyqxmd.com/read/28776104/heat-or-cold-priming-induced-cross-tolerance-to-abiotic-stresses-in-plants-key-regulators-and-possible-mechanisms
#4
REVIEW
Mohammad Anwar Hossain, Zhong-Guang Li, Tahsina Sharmin Hoque, David J Burritt, Masayuki Fujita, Sergi Munné-Bosch
Plants growing under field conditions are constantly exposed, either simultaneously or sequentially, to more than one abiotic stress factor. Plants have evolved sophisticated sensory systems to perceive a number of stress signals that allow them to activate the most adequate response to grow and survive in a given environment. Recently, cross-stress tolerance (i.e. tolerance to a second, strong stress after a different type of mild primary stress) has gained attention as a potential means of producing stress-resistant crops to aid with global food security...
August 4, 2017: Protoplasma
https://www.readbyqxmd.com/read/28744694/structural-and-functional-diversity-of-caspase-homologues-in-non-metazoan-organisms
#5
REVIEW
Marina Klemenčič, Christiane Funk
Caspases, the proteases involved in initiation and execution of metazoan programmed cell death, are only present in animals, while their structural homologues can be found in all domains of life, spanning from simple prokaryotes (orthocaspases) to yeast and plants (metacaspases). All members of this wide protease family contain the p20 domain, which harbours the catalytic dyad formed by the two amino acid residues, histidine and cysteine. Despite the high structural similarity of the p20 domain, metacaspases and orthocaspases were found to exhibit different substrate specificities than caspases...
July 25, 2017: Protoplasma
https://www.readbyqxmd.com/read/28741141/detonation-nanodiamonds-are-promising-nontoxic-delivery-system-for-urothelial-cells
#6
Daša Zupančič, Mateja Erdani Kreft, Maja Grdadolnik, Dimitar Mitev, Aleš Iglič, Peter Veranič
Detonation nanodiamonds (DNDs) are carbon-based nanomaterials that are among the most promising nanoparticles available for biomedical applications so far. This is due to their biocompatibility, which could be contributed to their inert core and conformable surface nature. However, DNDs cytotoxicity for urothelial cells and the routes of their internalization remains an open question in the aspect of nanodiamond surface. We therefore analyzed four types of DNDs for cytotoxicity and internalization with normal urothelial cells and two types of cancer urothelial cell lines in vitro...
July 24, 2017: Protoplasma
https://www.readbyqxmd.com/read/28730515/selective-induction-of-dna-damage-g2-abrogation-and-mitochondrial-apoptosis-by-leaf-extract-of-traditional-medicinal-plant-wrightia-arborea-in-k562-cells
#7
T Lakshmipriya, T Soumya, P R Jayasree, P R Manish Kumar
Plants have proved to be an important source of anti-cancer drugs. Wrightia arborea, an Indian Ayurvedic medicinal plant, is used traditionally to treat a variety of ailments. This study evaluates the antiproliferative/apoptotic potential of Wrightia arborea leaf extracts, prepared in different organic solvents, on cancer cell lines. MTT assay, light and fluorescence microscopy, flow cytometry, DNA laddering, alkaline comet assay, and western blotting were some of the techniques used for evaluation. Combinations of camptothecin, either with CHK1 inhibitor-PD407824 or with W...
July 20, 2017: Protoplasma
https://www.readbyqxmd.com/read/28718010/peter-sitte-1929-2015-a-theistic-cell-biologist
#8
EDITORIAL
Ulrich Kutschera, Peter Nick
No abstract text is available yet for this article.
July 17, 2017: Protoplasma
https://www.readbyqxmd.com/read/28718009/relative-salinity-tolerance-of-rice-cultivars-native-to-north-east-india-a-physiological-biochemical-and-molecular-perspective
#9
Takhellambam Omisun, Smita Sahoo, Bedabrata Saha, Sanjib Kumar Panda
Salinity is the second most prevalent abiotic stress faced by plants, and rice is not an exception. Through this study, it has been tried upon, to study the relative salinity tolerance of eight local varieties of North East India. Preliminary screening was based on their dose- and time-dependent physiological responses to salinity stress. Among the cultivars, Tampha was found to be relatively more tolerant, whereas MSE9 the most sensitive. To further ascertain their tolerance capacity, MDA and H2O2 content was determined, which also confirmed the tolerance level of the two cultivars...
July 17, 2017: Protoplasma
https://www.readbyqxmd.com/read/28710664/role-of-sodium-ion-transporters-and-osmotic-adjustments-in-stress-alleviation-of-cynodon-dactylon-under-nacl-treatment-a-parallel-investigation-with-rice
#10
Swarnendu Roy, Usha Chakraborty
Comparative analyses of the responses to NaCl in Cynodon dactylon and a sensitive crop species like rice could effectively unravel the salt tolerance mechanism in the former. C. dactylon, a wild perennial chloridoid grass having a wide range of ecological distribution is generally adaptable to varying degrees of salinity stress. The role of salt exclusion mechanism present exclusively in the wild grass was one of the major factors contributing to its tolerance. Salt exclusion was found to be induced at 4 days when the plants were treated with a minimum conc...
July 15, 2017: Protoplasma
https://www.readbyqxmd.com/read/28702759/evaluation-of-dna-damage-in-tobacco-male-meiocytes-involved-in-cytomixis-using-comet-assay
#11
Sergey Mursalimov, Alla Zagorskaya, Elena Deineko
Cytomixis is a process of nuclear migration between plant cells. As a rule, it is detectable in male meiocytes and gives rise to the cells with micronuclei. Examination of the integrity and functional state of migrating chromatin is of great interest, since cytomixis is assumed to change the gamete karyotype. We, for the first time, used comet assay to assess the DNA integrity in the chromatin that migrates between plant meiocytes. As was shown, the cells involved in cytomixis are viable and display no signs of DNA damage...
July 12, 2017: Protoplasma
https://www.readbyqxmd.com/read/28699026/aminolevulinic-acid-and-nitric-oxide-regulate-oxidative-defense-and-secondary-metabolisms-in-canola-brassica-napus-l-under-drought-stress
#12
Nudrat Aisha Akram, Majid Iqbal, Atta Muhammad, Muhammad Ashraf, Fahad Al-Qurainy, Sidra Shafiq
To minimize the damaging effects of stresses, plant growth regulators (PGRs) are widely used to sustain the plant life under stress-prone environments. So, a study was carried out to evaluate the response of two canola (Brassica napus L.) cultivars, Dunkeld and Cyclone, to foliar-applied two potential PGRs, nitric oxide (NO) and 5-aminolevulinic acid, under water deficit conditions. In this study, the levels of NO and ALA used were 0.02 and 0.895 mM, respectively. Plants of both canola cultivars were subjected to control (100% field capacity) and water deficit (60% field capacity)...
July 12, 2017: Protoplasma
https://www.readbyqxmd.com/read/28699025/polyamines-increase-nitric-oxide-and-reactive-oxygen-species-in-guard-cells-of-arabidopsis-thaliana-during-stomatal-closure
#13
Srinivas Agurla, Gunja Gayatri, Agepati S Raghavendra
A comprehensive study which was undertaken on the effect of three polyamines (PAs) on stomatal closure was examined in relation to nitric oxide (NO) and reactive oxygen species (ROS) levels in guard cells of Arabidopsis thaliana. Three PAs-putrescine (Put), spermidine (Spd), and spermine (Spm)-induced stomatal closure, while increasing the levels of NO as well as ROS in guard cells. The roles of NO and ROS were confirmed by the reversal of closure by cPTIO (NO scavenger) and catalase (ROS scavenger). The presence of L-NAME (NOS-like enzyme inhibitor) reversed PA-induced stomatal closure, suggesting that NOS-like enzyme played a significant role in NO production during stomatal closure...
July 11, 2017: Protoplasma
https://www.readbyqxmd.com/read/28667412/exogenous-salicylic-acid-mediated-modulation-of-arsenic-stress-tolerance-with-enhanced-accumulation-of-secondary-metabolites-and-improved-size-of-glandular-trichomes-in-artemisia-annua-l
#14
Anjana Kumari, Neha Pandey, Shashi Pandey-Rai
The present study was undertaken to find out individual and interactive effects of arsenic (As) and salicylic acid (SA) on an important medicinal plant, Artemisia annua. As uptake and its accumulation was detected and found to be maximum in roots at higher As concentration (150 μM). Under As treatments, H2O2 and MDA content were induced. Biomass and chlorophyll content were negatively affected under As treatments. Furthermore, enzymatic (SOD, CAT, APX, and GR) and non-enzymatic antioxidants were also enhanced under As treatments...
June 30, 2017: Protoplasma
https://www.readbyqxmd.com/read/28667411/symbiotic-microorganisms-in-puto-superbus-leonardi-1907-insecta-hemiptera-coccomorpha-putoidae
#15
Teresa Szklarzewicz, Małgorzata Kalandyk-Kołodziejczyk, Katarzyna Michalik, Władysława Jankowska, Anna Michalik
The scale insect Puto superbus (Putoidae) lives in mutualistic symbiotic association with bacteria. Molecular phylogenetic analyses have revealed that symbionts of P. superbus belong to the gammaproteobacterial genus Sodalis. In the adult females, symbionts occur both in the bacteriocytes constituting compact bacteriomes and in individual bacteriocytes, which are dispersed among ovarioles. The bacteriocytes also house a few small, rod-shaped Wolbachia bacteria in addition to the numerous large, elongated Sodalis-allied bacteria...
June 30, 2017: Protoplasma
https://www.readbyqxmd.com/read/28667410/pollen-wall-ontogeny-in-polemonium-caeruleum-polemoniaceae-and-suggested-underlying-mechanisms-of-development
#16
Valentina V Grigorjeva, Nina Gabarayeva
By a detailed ontogenetic study of Polemonium caeruleum pollen, tracing each stage of development at high TEM resolution, we aim to understand the establishment of the pollen wall and to unravel the mechanisms underlying sporoderm development. The main steps of exine ontogeny in Polemonium caeruleum, observed in the microspore periplasmic space, are spherical units, gradually transforming into columns, then to rod-like units (procolumellae), the appearance of the initial tectum, growth of columellae in height and tectum in thickness and initial sporopollenin accumulation on them, the appearance of the endexine lamellae and of dark-contrasted particles on the tectum, the appearance of a sponge-like layer and of the intine in aperture sites, the appearance of the foot layer on the base of the sponge-like layer and of spinules on the tectum, and massive sporopollenin accumulation...
June 30, 2017: Protoplasma
https://www.readbyqxmd.com/read/28653245/muscadinia-rotundifolia-noble-defense-response-to-plasmopara-viticola-inoculation-by-inducing-phytohormone-mediated-stilbene-accumulation
#17
Chaoxia Wang, Jiao Wu, Yali Zhang, Jiang Lu
Downy mildew (DM), one of the most devastating grape diseases worldwide, is caused by the biotrophic oomycete Plasmopara viticola (Pv). In general, grapevine responds to Pv infection with the accumulation of phytoalexins as part of the innate immune system, and diverse phytoalexins are induced on grapevines with different DM-resistance levels in response to Pv invasion. However, the regulation of phytoalexin biosynthesis during grapevine against Pv is still unclear. Herein, we detected stilbenes by UPLC-ESI-MS/MS and found that resveratrol was accumulated to higher level and earlier in the DM-immune Muscadinia rotundifolia 'Noble' than that in the DM-susceptible Vitis vinifera 'Thompson Seedless' after Pv inoculation...
June 26, 2017: Protoplasma
https://www.readbyqxmd.com/read/28643085/exogenous-application-of-nitric-oxide-modulates-osmolyte-metabolism-antioxidants-enzymes-of-ascorbate-glutathione-cycle-and-promotes-growth-under-cadmium-stress-in-tomato
#18
Parvaiz Ahmad, Mohammed Abass Ahanger, Mohammed Nasser Alyemeni, Leonard Wijaya, Pravej Alam
Experiments were carried out to investigate the role of nitric oxide (NO) in ameliorating the negative effects of cadmium stress in tomato seedlings. Plants treated with cadmium (CdCl2, 150 μM) showed reduced growth, biomass yield, pigment content, chlorophyll fluorescence, and gas exchange parameters. Exogenous application of NO donor (sodium nitroprusside) with nutrient solution protected chlorophyll pigments, restored chlorophyll fluorescence and gas exchange parameters, and caused significant enhancements in growth and biomass yield...
June 22, 2017: Protoplasma
https://www.readbyqxmd.com/read/28643084/influence-of-knockout-of-at4g20990-gene-encoding-%C3%AE-ca4-on-photosystem-ii-light-harvesting-antenna-in-plants-grown-under-different-light-intensities-and-day-lengths
#19
Natalia N Rudenko, Tatyana P Fedorchuk, Daria V Vetoshkina, Elena M Zhurikova, Lyudmila K Ignatova, Boris N Ivanov
Effect of knockout of the At4g20990 gene encoding α-carbonic anhydrase 4 (α-CA4) in Arabidopsis thaliana in plants grown in low light (LL, 80 μmol photons m(-2) s(-1)) or in high light (HL, 400 μmol photons m(-2) s(-1)) under long (LD, 16 h) or short (SD, 8 h) day length was studied. In α-CA4 knockout plants, under all studied conditions, the non-photochemical quenching was lower; the decrease was more pronounced under HL. This pointed to α-CA4 implication in the processes leading to energy dissipation in PSII antenna...
June 22, 2017: Protoplasma
https://www.readbyqxmd.com/read/28620698/potential-disease-biomarkers-dipeptidyl-peptidase-4-and-fibroblast-activation-protein
#20
REVIEW
Roger Yazbeck, Simone E Jaenisch, Catherine A Abbott
The importance of the dipeptidyl peptidase 4 (DPP4) gene family in regulating critical biochemical pathways continues to emerge. The two most well-studied members of the family, DPP4 and fibroblast activation protein (FAP), have been investigated both as therapeutic targets for disease and as diagnostic biomarkers. The interest in DPP4 and FAP as potential disease biomarkers has been driven primarily by observations of altered expression profiles in inflammatory diseases and cancer. Furthermore, the stability and persistence of soluble DPP4 and FAP in the serum make them attractive candidate serology markers...
June 16, 2017: Protoplasma
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