journal
https://read.qxmd.com/read/38452550/biodiversity-of-autotrophic-euglenids-based-on-the-group-specific-dna-metabarcoding-approach
#1
JOURNAL ARTICLE
Katarzyna Jankowska, Maja Łukomska-Kowalczyk, Rafał Milanowski, Alicja Fells, Bożena Zakryś
This study reports a comprehensive analysis of photoautotrophic euglenids' distribution and biodiversity in 16 small water bodies of various types (including fish ponds, field ponds, rural ponds and park ponds) located in three regions of Poland: Masovia, Masuria and Pomerania during a period of three years. By employing a euglenid specific barcode marker and a curated database of V2 18S rDNA sequences it was possible to identify 97.7 % of euglenid reads at species level. A total of 152 species classified in 13 genera were identified...
March 4, 2024: Protist
https://read.qxmd.com/read/38368650/dna-barcodes-reliably-differentiate-between-nivicolous-species-of-diderma-myxomycetes-amoebozoa-and-reveal-regional-differences-within-eurasia
#2
JOURNAL ARTICLE
Oleg N Shchepin, Ángela López Villalba, Maho Inoue, Ilya S Prikhodko, Daria A Erastova, Mikhail V Okun, Jan Woyzichovski, Yuka Yajima, Vladimir I Gmoshinskiy, Gabriel Moreno, Yuri K Novozhilov, Martin Schnittler
The nivicolous species of the genus Diderma are challenging to identify, and there are several competing views on their delimitation. We analyzed 102 accessions of nivicolous Diderma spp. that were sequenced for two or three unlinked genes to determine which of the current taxonomic treatments is better supported by molecular species delimitation methods. The results of a haplotype web analysis, Bayesian species delimitation under a multispecies coalescent model, and phylogenetic analyses on concatenated alignments support a splitting approach that distinguishes six taxa: Diderma alpinum, D...
February 10, 2024: Protist
https://read.qxmd.com/read/38350283/morpho-molecular-and-environmental-evidence-of-the-ocurrence-of-karenia-longicanalis-dinophyceae-kareniaceae-as-a-bloom-former-in-the-eastern-pacific-ocean
#3
JOURNAL ARTICLE
Alexis Escarcega-Bata, María Luisa Núñez Resendiz, María Eugenia Zamudio-Resendiz, Kurt M Dreckmann, Estefany Cuevas Sánchez, Abel Sentíes
Karenia longicanalis, an athecate dinoflagellate, was first described during a bloom in Victoria Harbour (Hong Kong, China). This study confirms the presence of K. longicanalis as a bloom former in the eastern Pacific Ocean. Specimens were collected in March 2019 at three sampling stations in Acapulco Bay, Mexico. Water temperature, salinity, dissolved oxygen, and pH were measured in situ at the time of sample collection. Cell morphology was examined by optical and scanning electron microscopy. A molecular analysis based on the amplification of the large subunit (LSU) rDNA region revealed that the LSU sequences formed a monophyletic group with other GenBank sequences belonging to K...
February 7, 2024: Protist
https://read.qxmd.com/read/38350284/dna-metabarcoding-reveals-the-impact-of-cu-2-on-soil-cercozoan-diversity
#4
JOURNAL ARTICLE
Leah Lourenço, Sara Ellegaard Bager, Duncan Y K Ng, Sanea Sheikh, Nikolaj Lunding Kindtler, Ida Broman Nielsen, Tobias Guldberg Frøslev, Flemming Ekelund
Although copper (Cu2+ ) is a micronutrient, the metal may be toxic if present in high concentrations in soil ecosystems and subsequently affect various organisms, ranging from microorganisms to earthworms. We performed a microcosm study with an array of Cu2+ concentrations, with a specific focus on Cercozoa, an important protozoan group in most soil food webs. Research on Cercozoa is still scarce in terms of both diversity and ecology; hence, to explore this group in more depth, we used high-throughput sequencing to detect Cu2+ induced community changes...
February 1, 2024: Protist
https://read.qxmd.com/read/38309038/phylogenetic-affiliation-of-pedinomonas-noctilucae-and-green-noctiluca-scintillans-nutritional-dynamics-in-the-gulf-of-mannar-southeastern-arabian-sea
#5
JOURNAL ARTICLE
Vajravelu Manigandan, Chandrasekaran Muthukumar, Chinmay Shah, Natarajan Logesh, Sanitha K Sivadas, Karri Ramu, M V Ramana Murthy
The present investigation focused on studying the phylogenetic position of the green Noctiluca endosymbiont, Pedinomonas noctilucae, collected from the Gulf of Mannar, India. In this study, we re-examined the evolutionary position of this endosymbiotic algae using rbcL sequences. The phylogenetic analysis revealed that P. noctilucae is distantly related to the Pedinomonas species, and formed a monophyletic clade with Marsupiomandaceae. Based on the phylogenetic association of endosymbiont with Maruspiomonadales it was concluded that the endosymbiont belongs to an independent genus within the family Marsupiomonadaceae...
January 30, 2024: Protist
https://read.qxmd.com/read/38301533/discovery-of-a-new-photosynthetic-euglenoid-in-poland-euglena-mazurica-sp-nov-euglenales-euglenaceae
#6
JOURNAL ARTICLE
Bożena Zakryś, Katarzyna Jankowska, Anna Majerowicz, Alicja Fells, Maja Łukomska-Kowalczyk
Herein we describe a new photosynthetic euglenoid species found in Poland - Euglena mazurica. A large population exists in a small, eutrophic body of water located in a pasture near Mikołajki town inside the Masurian Landscape Park (covering a part of the Masurian Lake District in Poland). The unique cell shape (corkscrew-like) discerns it well from other previously described euglenoid species with metabolic cells. The new species possesses two plate-like chloroplasts each with a pyrenoid accompanied by two paramylon caps placed on either side of it (diplopyrenoids)...
January 28, 2024: Protist
https://read.qxmd.com/read/38295671/ras-superfamily-gtpases-and-signal-transduction-in-euglena-gracilis
#7
JOURNAL ARTICLE
Mark C Field
Biological complexity is challenging to define, but can be considered through one or more features, including overall genome size, number of genes, morphological features, multicellularity, number of life cycle stages and the ability to adapt to different environments. Euglena gracilis meets several of these criteria, with a large genome of ∼38,000 protein coding genes and a considerable ability to survive under many different conditions, some of which can be described as challenging or harsh. Potential molecular exemplars of complexity tying these aspects together are signalling pathways, including GTPases, kinases and ubiquitylation, which increase the functionality of the gene-encoded proteome manyfold...
January 28, 2024: Protist
https://read.qxmd.com/read/38325049/single-cell-genomics-revealed-candidatus-grellia-alia-sp-nov-as-an-endosymbiont-of-eutreptiella-sp-euglenophyceae
#8
JOURNAL ARTICLE
Metody Hollender, Marta Sałek, Michał Karlicki, Anna Karnkowska
Though endosymbioses between protists and prokaryotes are widespread, certain host lineages have received disproportionate attention what may indicate either a predisposition to such interactions or limited studies on certain protist groups due to lack of cultures. The euglenids represent one such group in spite of microscopic observations showing intracellular bacteria in some strains. Here, we perform a comprehensive molecular analysis of a previously identified endosymbiont in the Eutreptiella sp. CCMP3347 using a single cell approach and bulk culture sequencing...
January 27, 2024: Protist
https://read.qxmd.com/read/38219636/on-saville-kent-s-a-manual-of-the-infusoria
#9
JOURNAL ARTICLE
John R Dolan
No abstract text is available yet for this article.
December 23, 2023: Protist
https://read.qxmd.com/read/38141417/morphogenesis-of-an-anaerobic-ciliate-heterometopus-palaeformis-kahl-1927-foissner-2016-ciliophora-armophorea-with-notes-on-its-morphological-and-molecular-characterization
#10
JOURNAL ARTICLE
Wenbao Zhuang, Xiaochen Feng, Ran Li, Saleh A Al-Farraj, Xiaozhong Hu
The morphology, morphogenesis, and molecular phylogeny of Heterometopus palaeformis (Kahl, 1927) Foissner, 2016 were studied using microscopical observations on live and protargol-stained specimens as well SSU rRNA gene sequencing. The morphogenetic data for the genus are presented for the first time. Compared to other metopids, the morphogenesis of H. palaeformis is distinct since its (1) perizonal stripe rows 4 and 5 are involved in the formation of the opisthe's adoral polykinetids; (2) perizonal stripe rows 3-5 and two adjacent preoral dome kineties contribute to most of the opisthe's paroral membrane while perizonal stripe rows 1 and 2 contribute very little; (3) four kinety rows are formed to the left of the opisthe's adoral zone of polykinetids...
December 16, 2023: Protist
https://read.qxmd.com/read/38118390/boosting-algicidal-efficiency-of-alteromonas-sp-fdhy-cj-against-skeletonema-costatum-through-fermentation-optimization
#11
JOURNAL ARTICLE
Pin Lv, Xinguo Shi, Qianqian Wang, Yuying Zhong, Yisong Guo, Jianfeng Chen
Algicidal bacteria exhibit promising potential against harmful algal blooms (HABs); however, their application has been limited due to their limited algicidal activity. This study demonstrates the enhanced algicidal activity of Alteromonas sp. FDHY-CJ bacteria against harmful Skeletonema costatum using a 5 L fermenter. Utilizing this refined framework increased the OD600 value and algal cell mortality by 6.50 and 2.88 times, respectively, compared to non-optimized culture cultivated in a flask using marine broth 2216E medium...
December 12, 2023: Protist
https://read.qxmd.com/read/38039844/gleaning-euglenozoa-specific-dna-polymerases-in-public-single-cell-transcriptome-data
#12
JOURNAL ARTICLE
Ryo Harada, Yuji Inagaki
Multiple genes encoding family A DNA polymerases (famA DNAPs), which are evolutionary relatives of DNA polymerase I (PolI) in bacteria and phages, have been found in eukaryotic genomes, and many of these proteins are used mainly in organelles. Among members of the phylum Euglenozoa, distinct types of famA DNAP, PolIA, PolIBCD+, POP, and eugPolA, have been found. It is intriguing how the suite of famA DNAPs had been established during the evolution of Euglenozoa, but the DNAP data have not been sampled from the taxa that sufficiently represent the diversity of this phylum...
November 25, 2023: Protist
https://read.qxmd.com/read/38041972/understanding-wax-ester-synthesis-in-euglena-gracilis-insights-into-mitochondrial-anaerobic-respiration
#13
JOURNAL ARTICLE
Masami Nakazawa, Hiroshi Inui
Euglena gracilis, photosynthetic protist, has a unique ability to generate wax esters in the absence of oxygen, employing a distinctive fatty acid synthesis mechanism. Through comprehensive inhibitor assays and gene-silencing techniques, our research clearly emphasized the indispensable role of the mitochondrial anaerobic respiratory chain in this biosynthesis. We identified acyl-CoA dehydrogenase, electron transfer flavoprotein (ETF), and rhodoquinone (RQ) as central molecular components in the pathway. These findings strongly indicated a potential reversal of beta-oxidation occurring within mitochondria for fatty acid production in anaerobic conditions...
November 21, 2023: Protist
https://read.qxmd.com/read/37995544/first-record-of-colpodidium-caudatum-ciliophora-colpodidiidae-in-europe
#14
JOURNAL ARTICLE
Juan Rodero Madrid, José Luis Olmo, Genoveva F Esteban
The present study documents the discovery of the first European population of Colpodidium caudatum (Ciliophora, Colpodidiidae) in a water drain in a school playground in Manzanares (Ciudad Real, Spain). This species has been documented on every continent except Antarctica and Europe, until now. The ciliate was isolated from wet run-off soil collected from the water drain and was grown in semi-permanent cultures in the laboratory. The infraciliature of the ciliate was revealed using silver carbonate impregnation and cell measurements were taken from living and silver-impregnated specimens...
November 8, 2023: Protist
https://read.qxmd.com/read/37935085/a-three-genome-ultraconserved-element-phylogeny-of-cryptophytes
#15
JOURNAL ARTICLE
Matthew J Greenwold, Kristiaän Merritt, Tammi L Richardson, Jeffry L Dudycha
Cryptophytes are single celled protists found in all aquatic environments. They are composed of a heterotrophic genus, Goniomonas, and a largely autotrophic group comprising many genera. Cryptophytes evolved through secondary endosymbiosis between a host eukaryotic heterotroph and a symbiont red alga. This merger resulted in a four-genome system that includes the nuclear and mitochondrial genomes from the host and a second nuclear genome (nucleomorph) and plastid genome inherited from the symbiont. Here, we make use of different genomes (with potentially distinct evolutionary histories) to perform a phylogenomic study of the early history of cryptophytes...
November 2, 2023: Protist
https://read.qxmd.com/read/37844389/ciliates-from-the-faeces-of-the-free-ranging-dromedary-from-oman-morphology-and-molecular-phylogeny
#16
JOURNAL ARTICLE
Olga A Kornilova, Anna I Ganyukova, Maria E Belokon, Vladimir V Platonov, Ludmila V Chistyakova
Ciliates Infundibulorium cameli from the faeces of the free-ranging dromedary from Oman were studied using a set of methods of the light and immunofluorescence microscopy and molecular phylogeny. With the use of molecular genetic methods, it was confirmed that the cysts found in the samples simultaneously with trophozoites actually belong to the species I. cameli. Tubulin cytoskeleton organization of trophozoites and cysts of this species were described for the first time. A striking morphological similarity between species I...
October 10, 2023: Protist
https://read.qxmd.com/read/37738738/genomic-architecture-constrains-macromolecular-allocation-in-dinoflagellates
#17
JOURNAL ARTICLE
Olga Carnicer, Ying-Yu Hu, Vinitha Ebenezer, Andrew J Irwin, Zoe V Finkel
Dinoflagellate genomes have a unique architecture that may constrain their physiological and biochemical responsiveness to environmental stressors. Here we quantified how nitrogen (N) starvation influenced macromolecular allocation and C:N:P of three photosynthetic marine dinoflagellates, representing different taxonomic classes and genome sizes. Dinoflagellates respond to nitrogen starvation by decreasing cellular nitrogen, protein and RNA content, but unlike many other eukaryotic phytoplankton examined RNA:protein is invariant...
September 12, 2023: Protist
https://read.qxmd.com/read/37573812/taxonomy-and-morphology-of-calciopappus-curvus-sp-nov-syracosphaeraceae-prymnesiophyceae-a-novel-appendage-bearing-coccolithophore
#18
JOURNAL ARTICLE
Odysseas A Archontikis, Josué G Millán, Harald Andruleit, Lluïsa Cros, Annelies Kleijne, Mikal Heldal, Hai Doan-Nhu, Amos Winter, Leocadio Blanco-Bercial, Jeremy R Young
Based on scanning electron microscopy observations, a new species of the coccolithophore genus Calciopappus (Syracosphaeraceae, Prymnesiophyceae) is described from the surface waters off Bergen and from the lower photic zone of sub-tropical and tropical waters. Morphological, coccolith rim structure and biometric analyses strongly support separation of this morphotype from the two described Calciopappus species, but inclusion of it within the genus. The new form differs from the other species in being noticeably smaller and in morpho-structural details of each of the three coccolith types that form the coccosphere: (1) the body coccoliths have an open central area; (2) the whorl coccoliths have a wide central opening and two thumb-like protrusions; and (3) the appendage coccoliths are curved...
July 28, 2023: Protist
https://read.qxmd.com/read/37454425/protozoa-in-the-remarkable-wall-charts-of-leuckart-and-nitsche-s-zoologische-wandtaflen
#19
JOURNAL ARTICLE
John Richard Dolan
No abstract text is available yet for this article.
July 14, 2023: Protist
https://read.qxmd.com/read/37453254/taxonomy-and-molecular-phylogeny-of-pleurostomatid-ciliates-from-china-with-a-description-of-two-new-species
#20
JOURNAL ARTICLE
Gongaote Zhang, Yuan Li, Ruitao Gong, Yu Qiao, Saleh A Al-Farraj, Hongbo Pan, Zhe Wang, Hunter N Hines
Ciliates in the order Pleurostomatida are found free-living in many habitats including within biofilms, but some (e.g. Pseudoamphileptus spp.) are ectocommensal on various hosts. Due to issues involving overall undersampling, the exact diversity and molecular phylogeny of this group remain largely underexplored. To combat this deficiency, detailed investigations were undertaken in northern China. As a result of these studies, we provide the morphological descriptions of two new species. Pseudoamphileptus apomacrostoma sp...
June 30, 2023: Protist
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