Shupeng Zhang, Yongpei Lu, Yanling Liu, Qin Kang, Qin Yao, Yingzi Li, Shuhuang Chen, Youlian Liu, Jiayi Du, Yixiao Xiong, Wenwen Zhu, Defang Chen, Xin Zhang, Ni Tang, Zhiqiong Li
Emerging findings point to a role for C1q/TNF-related protein 4 (CTRP4) in feeding in mammals. However, it remains unknown whether CTRP4 regulates feeding in fish. This study aimed to determine the feeding regulation function of CTRP4 in Siberian sturgeon (Acipenser baerii). In this study, the Siberian sturgeon ctrp4 (Abctrp4) gene was cloned, and Abctrp4 mRNA was shown to be highly expressed in the hypothalamus. In the hypothalamus, Abctrp4 mRNA decreased during fasting and reversed after refeeding. Subsequently, we obtained the AbCTRP4 recombinant protein by prokaryotic expression and optimized the expression and purification conditions...
January 6, 2024: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology